{"month":"12","article_processing_charge":"No","date_updated":"2023-08-03T14:21:51Z","pmid":1,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)"},"type":"journal_article","file":[{"date_updated":"2023-01-23T10:18:48Z","file_id":"12341","success":1,"date_created":"2023-01-23T10:18:48Z","file_size":5299475,"relation":"main_file","checksum":"073267a9c0ad9f85a650053bc7b23777","content_type":"application/pdf","file_name":"2022_Immunity_Petzold.pdf","creator":"dernst","access_level":"open_access"}],"day":"13","department":[{"_id":"MiSi"}],"abstract":[{"lang":"eng","text":"Intravascular neutrophils and platelets collaborate in maintaining host integrity, but their interaction can also trigger thrombotic complications. We report here that cooperation between neutrophil and platelet lineages extends to the earliest stages of platelet formation by megakaryocytes in the bone marrow. Using intravital microscopy, we show that neutrophils “plucked” intravascular megakaryocyte extensions, termed proplatelets, to control platelet production. Following CXCR4-CXCL12-dependent migration towards perisinusoidal megakaryocytes, plucking neutrophils actively pulled on proplatelets and triggered myosin light chain and extracellular-signal-regulated kinase activation through reactive oxygen species. By these mechanisms, neutrophils accelerate proplatelet growth and facilitate continuous release of platelets in steady state. Following myocardial infarction, plucking neutrophils drove excessive release of young, reticulated platelets and boosted the risk of recurrent ischemia. Ablation of neutrophil plucking normalized thrombopoiesis and reduced recurrent thrombosis after myocardial infarction and thrombus burden in venous thrombosis. We establish neutrophil plucking as a target to reduce thromboischemic events."}],"date_published":"2022-12-13T00:00:00Z","publication_status":"published","issue":"12","publisher":"Elsevier","scopus_import":"1","oa_version":"Published Version","oa":1,"date_created":"2023-01-12T11:56:54Z","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","quality_controlled":"1","title":"Neutrophil “plucking” on megakaryocytes drives platelet production and boosts cardiovascular disease","status":"public","external_id":{"pmid":["36272416"],"isi":["000922019600003"]},"publication_identifier":{"issn":["1074-7613"]},"has_accepted_license":"1","author":[{"full_name":"Petzold, Tobias","last_name":"Petzold","first_name":"Tobias"},{"first_name":"Zhe","last_name":"Zhang","full_name":"Zhang, Zhe"},{"full_name":"Ballesteros, Iván","first_name":"Iván","last_name":"Ballesteros"},{"full_name":"Saleh, Inas","first_name":"Inas","last_name":"Saleh"},{"full_name":"Polzin, Amin","last_name":"Polzin","first_name":"Amin"},{"full_name":"Thienel, Manuela","last_name":"Thienel","first_name":"Manuela"},{"last_name":"Liu","first_name":"Lulu","full_name":"Liu, Lulu"},{"full_name":"Ul Ain, Qurrat","first_name":"Qurrat","last_name":"Ul Ain"},{"full_name":"Ehreiser, Vincent","last_name":"Ehreiser","first_name":"Vincent"},{"full_name":"Weber, Christian","last_name":"Weber","first_name":"Christian"},{"full_name":"Kilani, Badr","first_name":"Badr","last_name":"Kilani"},{"full_name":"Mertsch, Pontus","last_name":"Mertsch","first_name":"Pontus"},{"last_name":"Götschke","first_name":"Jeremias","full_name":"Götschke, Jeremias"},{"first_name":"Sophie","last_name":"Cremer","full_name":"Cremer, Sophie"},{"full_name":"Fu, Wenwen","last_name":"Fu","first_name":"Wenwen"},{"last_name":"Lorenz","first_name":"Michael","full_name":"Lorenz, Michael"},{"first_name":"Hellen","last_name":"Ishikawa-Ankerhold","full_name":"Ishikawa-Ankerhold, Hellen"},{"full_name":"Raatz, Elisabeth","last_name":"Raatz","first_name":"Elisabeth"},{"last_name":"El-Nemr","first_name":"Shaza","full_name":"El-Nemr, Shaza"},{"first_name":"Agnes","last_name":"Görlach","full_name":"Görlach, Agnes"},{"first_name":"Esther","last_name":"Marhuenda","full_name":"Marhuenda, Esther"},{"full_name":"Stark, Konstantin","first_name":"Konstantin","last_name":"Stark"},{"full_name":"Pircher, Joachim","last_name":"Pircher","first_name":"Joachim"},{"full_name":"Stegner, David","first_name":"David","last_name":"Stegner"},{"first_name":"Christian","last_name":"Gieger","full_name":"Gieger, Christian"},{"full_name":"Schmidt-Supprian, Marc","first_name":"Marc","last_name":"Schmidt-Supprian"},{"orcid":"0000-0001-6120-3723","first_name":"Florian R","last_name":"Gärtner","full_name":"Gärtner, Florian R","id":"397A88EE-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Almendros, Isaac","last_name":"Almendros","first_name":"Isaac"},{"first_name":"Malte","last_name":"Kelm","full_name":"Kelm, Malte"},{"first_name":"Christian","last_name":"Schulz","full_name":"Schulz, Christian"},{"full_name":"Hidalgo, Andrés","first_name":"Andrés","last_name":"Hidalgo"},{"full_name":"Massberg, Steffen","first_name":"Steffen","last_name":"Massberg"}],"publication":"Immunity","page":"2285-2299.e7","doi":"10.1016/j.immuni.2022.10.001","_id":"12119","language":[{"iso":"eng"}],"article_type":"original","citation":{"ieee":"T. Petzold <i>et al.</i>, “Neutrophil ‘plucking’ on megakaryocytes drives platelet production and boosts cardiovascular disease,” <i>Immunity</i>, vol. 55, no. 12. Elsevier, p. 2285–2299.e7, 2022.","mla":"Petzold, Tobias, et al. “Neutrophil ‘Plucking’ on Megakaryocytes Drives Platelet Production and Boosts Cardiovascular Disease.” <i>Immunity</i>, vol. 55, no. 12, Elsevier, 2022, p. 2285–2299.e7, doi:<a href=\"https://doi.org/10.1016/j.immuni.2022.10.001\">10.1016/j.immuni.2022.10.001</a>.","short":"T. Petzold, Z. Zhang, I. Ballesteros, I. Saleh, A. Polzin, M. Thienel, L. Liu, Q. Ul Ain, V. Ehreiser, C. Weber, B. Kilani, P. Mertsch, J. Götschke, S. Cremer, W. Fu, M. Lorenz, H. Ishikawa-Ankerhold, E. Raatz, S. El-Nemr, A. Görlach, E. Marhuenda, K. Stark, J. Pircher, D. Stegner, C. Gieger, M. Schmidt-Supprian, F.R. Gärtner, I. Almendros, M. Kelm, C. Schulz, A. Hidalgo, S. Massberg, Immunity 55 (2022) 2285–2299.e7.","chicago":"Petzold, Tobias, Zhe Zhang, Iván Ballesteros, Inas Saleh, Amin Polzin, Manuela Thienel, Lulu Liu, et al. “Neutrophil ‘Plucking’ on Megakaryocytes Drives Platelet Production and Boosts Cardiovascular Disease.” <i>Immunity</i>. Elsevier, 2022. <a href=\"https://doi.org/10.1016/j.immuni.2022.10.001\">https://doi.org/10.1016/j.immuni.2022.10.001</a>.","apa":"Petzold, T., Zhang, Z., Ballesteros, I., Saleh, I., Polzin, A., Thienel, M., … Massberg, S. (2022). Neutrophil “plucking” on megakaryocytes drives platelet production and boosts cardiovascular disease. <i>Immunity</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.immuni.2022.10.001\">https://doi.org/10.1016/j.immuni.2022.10.001</a>","ista":"Petzold T, Zhang Z, Ballesteros I, Saleh I, Polzin A, Thienel M, Liu L, Ul Ain Q, Ehreiser V, Weber C, Kilani B, Mertsch P, Götschke J, Cremer S, Fu W, Lorenz M, Ishikawa-Ankerhold H, Raatz E, El-Nemr S, Görlach A, Marhuenda E, Stark K, Pircher J, Stegner D, Gieger C, Schmidt-Supprian M, Gärtner FR, Almendros I, Kelm M, Schulz C, Hidalgo A, Massberg S. 2022. Neutrophil “plucking” on megakaryocytes drives platelet production and boosts cardiovascular disease. Immunity. 55(12), 2285–2299.e7.","ama":"Petzold T, Zhang Z, Ballesteros I, et al. Neutrophil “plucking” on megakaryocytes drives platelet production and boosts cardiovascular disease. <i>Immunity</i>. 2022;55(12):2285-2299.e7. doi:<a href=\"https://doi.org/10.1016/j.immuni.2022.10.001\">10.1016/j.immuni.2022.10.001</a>"},"ec_funded":1,"year":"2022","intvolume":"        55","volume":55,"project":[{"_id":"260AA4E2-B435-11E9-9278-68D0E5697425","name":"Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells","grant_number":"747687","call_identifier":"H2020"}],"ddc":["570"],"file_date_updated":"2023-01-23T10:18:48Z","isi":1,"acknowledgement":"We thank Coung Kieu and Dominik van den Heuvel for excellent technical assistance. This work was supported by the German Research Foundation (PE2704/2-1, PE2704/3-1 to T.P., SFB 1123-project B06 to S.M., SFB1525 project A07 to D.S, TRR 332 project A7 to C.S., PO 2247/2-1 to A.P., SFB1116-project B11 to A.P. and B12 to M.K.), LMU Munich’s Institutional\r\nStrategy LMUexcellent within the framework of the German Excellence Initiative (No. 806 32 006 to T.P.), and by the German Centre for Cardiovascular Research (DZHK) to T.P. (Postdoc Start-up grant No. 100378833). This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant agreement No. 833440 to S.M.). F.G. received funding from the European Union’s\r\nHorizon 2020 research and innovation program under the Marie Sk1odowska-Curie grant agreement no. 747687. A.H. was funded by RTI2018-095497-B-I00 from Ministerio de Ciencia e Innovacio´ n (MICINN), HR17_00527 from Fundacion La Caixa, and Transatlantic Network of Excellence (TNE-18CVD04) from the Leducq Foundation. The CNIC is supported by the MICINN and the Pro CNIC Foundation and is a Severo Ochoa Center of Excellence (CEX2020-001041-S). A.P. was supported by the Forschungskommission of the Medical Faculty of the Heinrich-Heine-Universität Düsseldorf (No. 18-2019 to A.P.). C.G. was supported by the Helmholtz Alliance ‘Aging and Metabolic Programming, AMPro,’ by the German Federal\r\nMinistry of Education and Research to the German Center for Diabetes Research (DZD), and by the Bavarian State Ministry of Health and Care through the research project DigiMed Bayern.","keyword":["Infectious Diseases","Immunology","Immunology and Allergy"]}