{"issue":"10","date_published":"2022-08-19T00:00:00Z","publication_status":"published","abstract":[{"text":"Endocytosis is a multistep process involving the sequential recruitment and action of numerous proteins. This process can be divided into two phases: an early phase, in which sites of endocytosis are formed, and a late phase in which clathrin-coated vesicles are formed and internalized into the cytosol, but how these phases link to each other remains unclear. In this study, we demonstrate that anchoring the yeast Eps15-like protein Pan1p to the peroxisome triggers most of the events occurring during the late phase at the peroxisome. At this ectopic location, Pan1p recruits most proteins that function in the late phases—including actin nucleation promoting factors—and then initiates actin polymerization. Pan1p also recruited Prk1 kinase and actin depolymerizing factors, thereby triggering disassembly immediately after actin assembly and inducing dissociation of endocytic proteins from the peroxisome. These observations suggest that Pan1p is a key regulator for initiating, processing, and completing the late phase of endocytosis.","lang":"eng"}],"department":[{"_id":"DaSi"}],"day":"19","type":"journal_article","license":"https://creativecommons.org/licenses/by-nc-sa/4.0/","file":[{"date_updated":"2023-02-21T23:30:39Z","file_id":"12321","file_size":7816875,"date_created":"2023-01-20T09:32:53Z","relation":"main_file","checksum":"f2e581e66b5cdab9df81b56e850b3eaa","content_type":"application/pdf","file_name":"2022_JCB_Enshoji.pdf","embargo":"2023-02-20","creator":"dernst","access_level":"open_access"}],"date_updated":"2023-08-03T13:49:07Z","pmid":1,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode","short":"CC BY-NC-SA (4.0)","image":"/images/cc_by_nc_sa.png","name":"Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)"},"article_processing_charge":"No","month":"08","status":"public","title":"Eps15/Pan1p is a master regulator of the late stages of the endocytic pathway","quality_controlled":"1","date_created":"2022-09-11T22:01:54Z","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","oa_version":"Published Version","oa":1,"scopus_import":"1","publisher":"Rockefeller University Press","citation":{"ama":"Enshoji M, Miyano Y, Yoshida N, et al. Eps15/Pan1p is a master regulator of the late stages of the endocytic pathway. <i>Journal of Cell Biology</i>. 2022;221(10). doi:<a href=\"https://doi.org/10.1083/jcb.202112138\">10.1083/jcb.202112138</a>","apa":"Enshoji, M., Miyano, Y., Yoshida, N., Nagano, M., Watanabe, M., Kunihiro, M., … Toshima, J. (2022). Eps15/Pan1p is a master regulator of the late stages of the endocytic pathway. <i>Journal of Cell Biology</i>. Rockefeller University Press. <a href=\"https://doi.org/10.1083/jcb.202112138\">https://doi.org/10.1083/jcb.202112138</a>","chicago":"Enshoji, Mariko, Yoshiko Miyano, Nao Yoshida, Makoto Nagano, Minami Watanabe, Mayumi Kunihiro, Daria E Siekhaus, Junko Y. Toshima, and Jiro Toshima. “Eps15/Pan1p Is a Master Regulator of the Late Stages of the Endocytic Pathway.” <i>Journal of Cell Biology</i>. Rockefeller University Press, 2022. <a href=\"https://doi.org/10.1083/jcb.202112138\">https://doi.org/10.1083/jcb.202112138</a>.","ista":"Enshoji M, Miyano Y, Yoshida N, Nagano M, Watanabe M, Kunihiro M, Siekhaus DE, Toshima JY, Toshima J. 2022. Eps15/Pan1p is a master regulator of the late stages of the endocytic pathway. Journal of Cell Biology. 221(10), e202112138.","short":"M. Enshoji, Y. Miyano, N. Yoshida, M. Nagano, M. Watanabe, M. Kunihiro, D.E. Siekhaus, J.Y. Toshima, J. Toshima, Journal of Cell Biology 221 (2022).","mla":"Enshoji, Mariko, et al. “Eps15/Pan1p Is a Master Regulator of the Late Stages of the Endocytic Pathway.” <i>Journal of Cell Biology</i>, vol. 221, no. 10, e202112138, Rockefeller University Press, 2022, doi:<a href=\"https://doi.org/10.1083/jcb.202112138\">10.1083/jcb.202112138</a>.","ieee":"M. Enshoji <i>et al.</i>, “Eps15/Pan1p is a master regulator of the late stages of the endocytic pathway,” <i>Journal of Cell Biology</i>, vol. 221, no. 10. Rockefeller University Press, 2022."},"article_type":"original","_id":"12080","article_number":"e202112138","language":[{"iso":"eng"}],"has_accepted_license":"1","author":[{"full_name":"Enshoji, Mariko","last_name":"Enshoji","first_name":"Mariko"},{"full_name":"Miyano, Yoshiko","first_name":"Yoshiko","last_name":"Miyano"},{"last_name":"Yoshida","first_name":"Nao","full_name":"Yoshida, Nao"},{"first_name":"Makoto","last_name":"Nagano","full_name":"Nagano, Makoto"},{"first_name":"Minami","last_name":"Watanabe","full_name":"Watanabe, Minami"},{"first_name":"Mayumi","last_name":"Kunihiro","full_name":"Kunihiro, Mayumi"},{"orcid":"0000-0001-8323-8353","full_name":"Siekhaus, Daria E","id":"3D224B9E-F248-11E8-B48F-1D18A9856A87","last_name":"Siekhaus","first_name":"Daria E"},{"full_name":"Toshima, Junko Y.","last_name":"Toshima","first_name":"Junko Y."},{"full_name":"Toshima, Jiro","first_name":"Jiro","last_name":"Toshima"}],"publication":"Journal of Cell Biology","doi":"10.1083/jcb.202112138","publication_identifier":{"issn":["0021-9525"],"eissn":["1540-8140"]},"external_id":{"pmid":["35984332"],"isi":["000932770500001"]},"acknowledgement":"This work was supported by JSPS KAKENHI GRANT #18K062291, and the Takeda Science Foundation to J.Y. Toshima, as well as JSPS KAKENHI GRANT #19K065710, the Uehara Memorial Foundation, and Life Science Foundation of JAPAN to J. Toshima.","file_date_updated":"2023-02-21T23:30:39Z","isi":1,"ddc":["570"],"volume":221,"intvolume":"       221","year":"2022"}