{"main_file_link":[{"url":"https://doi.org/10.1016/j.devcel.2014.04.025","open_access":"1"}],"abstract":[{"lang":"eng","text":"Membrane phospholipids typically contain fatty acids (FAs) of 16 and 18 carbon atoms. This particular chain length is evolutionarily highly conserved and presumably provides maximum stability and dynamic properties to biological membranes in response to nutritional or environmental cues. Here, we show that the relative proportion of C16 versus C18 FAs is regulated by the activity of acetyl-CoA carboxylase (Acc1), the first and rate-limiting enzyme of FA de novo synthesis. Acc1 activity is attenuated by AMPK/Snf1-dependent phosphorylation, which is required to maintain an appropriate acyl-chain length distribution. Moreover, we find that the transcriptional repressor Opi1 preferentially binds to C16 over C18 phosphatidic acid (PA) species: thus, C16-chain containing PA sequesters Opi1 more effectively to the ER, enabling AMPK/Snf1 control of PA acyl-chain length to determine the degree of derepression of Opi1 target genes. These findings reveal an unexpected regulatory link between the major energy-sensing kinase, membrane lipid composition, and transcription."}],"issue":"6","publication_status":"published","date_published":"2014-06-23T00:00:00Z","extern":"1","date_updated":"2023-02-21T10:09:45Z","pmid":1,"article_processing_charge":"No","month":"06","day":"23","type":"journal_article","status":"public","title":"Regulation of gene expression through a transcriptional repressor that senses acyl-chain length in membrane phospholipids","quality_controlled":"1","scopus_import":"1","publisher":"Elsevier","date_created":"2022-08-25T08:42:42Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","oa_version":"Published Version","oa":1,"_id":"11968","language":[{"iso":"eng"}],"citation":{"ista":"Hofbauer HF, Schopf FH, Schleifer H, Knittelfelder OL, Pieber B, Rechberger GN, Wolinski H, Gaspar ML, Kappe CO, Stadlmann J, Mechtler K, Zenz A, Lohner K, Tehlivets O, Henry SA, Kohlwein SD. 2014. Regulation of gene expression through a transcriptional repressor that senses acyl-chain length in membrane phospholipids. Developmental Cell. 29(6), P729-739.","apa":"Hofbauer, H. F., Schopf, F. H., Schleifer, H., Knittelfelder, O. L., Pieber, B., Rechberger, G. N., … Kohlwein, S. D. (2014). Regulation of gene expression through a transcriptional repressor that senses acyl-chain length in membrane phospholipids. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2014.04.025\">https://doi.org/10.1016/j.devcel.2014.04.025</a>","chicago":"Hofbauer, Harald F., Florian H. Schopf, Hannes Schleifer, Oskar L. Knittelfelder, Bartholomäus Pieber, Gerald N. Rechberger, Heimo Wolinski, et al. “Regulation of Gene Expression through a Transcriptional Repressor That Senses Acyl-Chain Length in Membrane Phospholipids.” <i>Developmental Cell</i>. Elsevier, 2014. <a href=\"https://doi.org/10.1016/j.devcel.2014.04.025\">https://doi.org/10.1016/j.devcel.2014.04.025</a>.","ama":"Hofbauer HF, Schopf FH, Schleifer H, et al. Regulation of gene expression through a transcriptional repressor that senses acyl-chain length in membrane phospholipids. <i>Developmental Cell</i>. 2014;29(6):P729-739. doi:<a href=\"https://doi.org/10.1016/j.devcel.2014.04.025\">10.1016/j.devcel.2014.04.025</a>","short":"H.F. Hofbauer, F.H. Schopf, H. Schleifer, O.L. Knittelfelder, B. Pieber, G.N. Rechberger, H. Wolinski, M.L. Gaspar, C.O. Kappe, J. Stadlmann, K. Mechtler, A. Zenz, K. Lohner, O. Tehlivets, S.A. Henry, S.D. Kohlwein, Developmental Cell 29 (2014) P729-739.","mla":"Hofbauer, Harald F., et al. “Regulation of Gene Expression through a Transcriptional Repressor That Senses Acyl-Chain Length in Membrane Phospholipids.” <i>Developmental Cell</i>, vol. 29, no. 6, Elsevier, 2014, pp. P729-739, doi:<a href=\"https://doi.org/10.1016/j.devcel.2014.04.025\">10.1016/j.devcel.2014.04.025</a>.","ieee":"H. F. Hofbauer <i>et al.</i>, “Regulation of gene expression through a transcriptional repressor that senses acyl-chain length in membrane phospholipids,” <i>Developmental Cell</i>, vol. 29, no. 6. Elsevier, pp. P729-739, 2014."},"article_type":"original","publication_identifier":{"eissn":["1878-1551"],"issn":["1534-5807"]},"external_id":{"pmid":["24960695"]},"publication":"Developmental Cell","author":[{"first_name":"Harald F.","last_name":"Hofbauer","full_name":"Hofbauer, Harald F."},{"last_name":"Schopf","first_name":"Florian H.","full_name":"Schopf, Florian H."},{"last_name":"Schleifer","first_name":"Hannes","full_name":"Schleifer, Hannes"},{"last_name":"Knittelfelder","first_name":"Oskar L.","full_name":"Knittelfelder, Oskar L."},{"orcid":"0000-0001-8689-388X","id":"93e5e5b2-0da6-11ed-8a41-af589a024726","full_name":"Pieber, Bartholomäus","last_name":"Pieber","first_name":"Bartholomäus"},{"first_name":"Gerald N.","last_name":"Rechberger","full_name":"Rechberger, Gerald N."},{"full_name":"Wolinski, Heimo","last_name":"Wolinski","first_name":"Heimo"},{"full_name":"Gaspar, Maria L.","last_name":"Gaspar","first_name":"Maria L."},{"first_name":"C. Oliver","last_name":"Kappe","full_name":"Kappe, C. Oliver"},{"first_name":"Johannes","last_name":"Stadlmann","full_name":"Stadlmann, Johannes"},{"first_name":"Karl","last_name":"Mechtler","full_name":"Mechtler, Karl"},{"first_name":"Alexandra","last_name":"Zenz","full_name":"Zenz, Alexandra"},{"full_name":"Lohner, Karl","last_name":"Lohner","first_name":"Karl"},{"full_name":"Tehlivets, Oksana","first_name":"Oksana","last_name":"Tehlivets"},{"last_name":"Henry","first_name":"Susan A.","full_name":"Henry, Susan A."},{"full_name":"Kohlwein, Sepp D.","last_name":"Kohlwein","first_name":"Sepp D."}],"page":"P729-739","doi":"10.1016/j.devcel.2014.04.025","year":"2014","volume":29,"intvolume":"        29"}