{"external_id":{"pmid":["35861182"],"isi":["000837950900001"]},"publication_identifier":{"eissn":["2050-5701"],"issn":["2050-5698"]},"doi":"10.1093/jmicro/dfac037","page":"249-261","publication":"Microscopy","has_accepted_license":"1","author":[{"full_name":"Gerle, Christoph","first_name":"Christoph","last_name":"Gerle"},{"full_name":"Kishikawa, Jun-ichi","first_name":"Jun-ichi","last_name":"Kishikawa"},{"last_name":"Yamaguchi","first_name":"Tomoko","full_name":"Yamaguchi, Tomoko"},{"full_name":"Nakanishi, Atsuko","first_name":"Atsuko","last_name":"Nakanishi"},{"orcid":"0000-0002-3219-2022","last_name":"Çoruh","first_name":"Mehmet Orkun","id":"d25163e5-8d53-11eb-a251-e6dd8ea1b8ef","full_name":"Çoruh, Mehmet Orkun"},{"full_name":"Makino, Fumiaki","first_name":"Fumiaki","last_name":"Makino"},{"last_name":"Miyata","first_name":"Tomoko","full_name":"Miyata, Tomoko"},{"full_name":"Kawamoto, Akihiro","first_name":"Akihiro","last_name":"Kawamoto"},{"full_name":"Yokoyama, Ken","first_name":"Ken","last_name":"Yokoyama"},{"full_name":"Namba, Keiichi","last_name":"Namba","first_name":"Keiichi"},{"full_name":"Kurisu, Genji","last_name":"Kurisu","first_name":"Genji"},{"first_name":"Takayuki","last_name":"Kato","full_name":"Kato, Takayuki"}],"language":[{"iso":"eng"}],"_id":"11648","citation":{"chicago":"Gerle, Christoph, Jun-ichi Kishikawa, Tomoko Yamaguchi, Atsuko Nakanishi, Mehmet Orkun Çoruh, Fumiaki Makino, Tomoko Miyata, et al. “Structures of Multisubunit Membrane Complexes with the CRYO ARM 200.” <i>Microscopy</i>. Oxford University Press, 2022. <a href=\"https://doi.org/10.1093/jmicro/dfac037\">https://doi.org/10.1093/jmicro/dfac037</a>.","ista":"Gerle C, Kishikawa J, Yamaguchi T, Nakanishi A, Çoruh MO, Makino F, Miyata T, Kawamoto A, Yokoyama K, Namba K, Kurisu G, Kato T. 2022. Structures of multisubunit membrane complexes with the CRYO ARM 200. Microscopy. 71(5), 249–261.","apa":"Gerle, C., Kishikawa, J., Yamaguchi, T., Nakanishi, A., Çoruh, M. O., Makino, F., … Kato, T. (2022). Structures of multisubunit membrane complexes with the CRYO ARM 200. <i>Microscopy</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/jmicro/dfac037\">https://doi.org/10.1093/jmicro/dfac037</a>","ama":"Gerle C, Kishikawa J, Yamaguchi T, et al. Structures of multisubunit membrane complexes with the CRYO ARM 200. <i>Microscopy</i>. 2022;71(5):249-261. doi:<a href=\"https://doi.org/10.1093/jmicro/dfac037\">10.1093/jmicro/dfac037</a>","short":"C. Gerle, J. Kishikawa, T. Yamaguchi, A. Nakanishi, M.O. Çoruh, F. Makino, T. Miyata, A. Kawamoto, K. Yokoyama, K. Namba, G. Kurisu, T. Kato, Microscopy 71 (2022) 249–261.","mla":"Gerle, Christoph, et al. “Structures of Multisubunit Membrane Complexes with the CRYO ARM 200.” <i>Microscopy</i>, vol. 71, no. 5, Oxford University Press, 2022, pp. 249–61, doi:<a href=\"https://doi.org/10.1093/jmicro/dfac037\">10.1093/jmicro/dfac037</a>.","ieee":"C. Gerle <i>et al.</i>, “Structures of multisubunit membrane complexes with the CRYO ARM 200,” <i>Microscopy</i>, vol. 71, no. 5. Oxford University Press, pp. 249–261, 2022."},"article_type":"original","year":"2022","intvolume":"        71","volume":71,"ddc":["570"],"keyword":["Radiology","Nuclear Medicine and imaging","Instrumentation","Structural Biology"],"acknowledgement":"Cyclic Innovation for Clinical Empowerment (JP17pc0101020 from Japan Agency for Medical Research and Development (AMED) to K.N. and G.K.); Platform Project for Supporting Drug Discovery and Life Science Research (Basis for Supporting Innovative Drug Discovery and Life Science Research) from AMED (JP20am0101117 to K.N., JP16K07266 to Atsunori Oshima and C.G., JP22ama121001j0001 to Masaki Yamamoto, G.K., T.K. and C.G.); a JSPS KAHKENHI\r\ngrant (20K06514 to J.K.) and a Grant-in-aid for JSPS fellows (20J00162 to A.N.).\r\nWe are grateful for initiation and scientific support from Matthias Rogner, Marc M. Nowaczyk, Anna Frank and ̈Yuko Misumi for the PSI monomer project and also would like to thank Hideki Shigematsu for critical reading of the manuscript. And we are indebted to the two anonymous reviewers who helped us to improve our manuscript.","file_date_updated":"2023-02-03T08:34:48Z","isi":1,"article_processing_charge":"No","month":"10","tmp":{"image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"pmid":1,"date_updated":"2023-08-03T12:13:37Z","file":[{"date_updated":"2023-02-03T08:34:48Z","date_created":"2023-02-03T08:34:48Z","file_size":7812696,"file_id":"12498","success":1,"relation":"main_file","checksum":"23b51c163636bf9313f7f0818312e67e","file_name":"2022_Microscopy_Gerle.pdf","creator":"dernst","access_level":"open_access","content_type":"application/pdf"}],"type":"journal_article","day":"01","department":[{"_id":"LeSa"}],"abstract":[{"text":"Progress in structural membrane biology has been significantly accelerated by the ongoing 'Resolution Revolution' in cryo electron microscopy (cryo-EM). In particular, structure determination by single particle analysis has evolved into the most powerful method for atomic model building of multisubunit membrane protein complexes. This has created an ever increasing demand in cryo-EM machine time, which to satisfy is in need of new and affordable cryo electron microscopes. Here, we review our experience in using the JEOL CRYO ARM 200 prototype for the structure determination by single particle analysis of three different multisubunit membrane complexes: the Thermus thermophilus V-type ATPase VO complex, the Thermosynechococcus elongatus photosystem I monomer and the flagellar motor LP-ring from Salmonella enterica.","lang":"eng"}],"publication_status":"published","date_published":"2022-10-01T00:00:00Z","issue":"5","publisher":"Oxford University Press","scopus_import":"1","oa":1,"oa_version":"Published Version","date_created":"2022-07-25T10:04:58Z","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","title":"Structures of multisubunit membrane complexes with the CRYO ARM 200","quality_controlled":"1","status":"public"}