{"language":[{"iso":"eng"}],"intvolume":"       113","oa_version":"Published Version","issue":"2","quality_controlled":"1","scopus_import":"1","title":"The conserved Nup107-160 complex is critical for nuclear pore complex assembly","keyword":["General Biochemistry","Genetics and Molecular Biology"],"pmid":1,"date_published":"2003-04-17T00:00:00Z","day":"17","publisher":"Elsevier","year":"2003","article_type":"original","publication_identifier":{"issn":["0092-8674"]},"extern":"1","type":"journal_article","date_updated":"2022-07-18T08:57:42Z","volume":113,"abstract":[{"text":"Nuclear pore complexes (NPCs) are large multiprotein assemblies that allow traffic between the cytoplasm and the nucleus. During mitosis in higher eukaryotes, the Nuclear Envelope (NE) breaks down and NPCs disassemble. How NPCs reassemble and incorporate into the NE upon mitotic exit is poorly understood. We demonstrate a function for the conserved Nup107-160 complex in this process. Partial in vivo depletion of Nup133 or Nup107 via RNAi in HeLa cells resulted in reduced levels of multiple nucleoporins and decreased NPC density in the NE. Immunodepletion of the entire Nup107-160 complex from in vitro nuclear assembly reactions produced nuclei with a continuous NE but no NPCs. This phenotype was reversible only if Nup107-160 complex was readded before closed NE formation. Depletion also prevented association of FG-repeat nucleoporins with chromatin. We propose a stepwise model in which postmitotic NPC assembly initiates on chromatin via early recruitment of the Nup107-160 complex.","lang":"eng"}],"publication":"Cell","publication_status":"published","_id":"11122","author":[{"last_name":"Walther","full_name":"Walther, Tobias C.","first_name":"Tobias C."},{"full_name":"Alves, Annabelle","first_name":"Annabelle","last_name":"Alves"},{"first_name":"Helen","full_name":"Pickersgill, Helen","last_name":"Pickersgill"},{"last_name":"Loı̈odice","full_name":"Loı̈odice, Isabelle","first_name":"Isabelle"},{"full_name":"HETZER, Martin W","first_name":"Martin W","orcid":"0000-0002-2111-992X","last_name":"HETZER","id":"86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed"},{"full_name":"Galy, Vincent","first_name":"Vincent","last_name":"Galy"},{"full_name":"Hülsmann, Bastian B.","first_name":"Bastian B.","last_name":"Hülsmann"},{"full_name":"Köcher, Thomas","first_name":"Thomas","last_name":"Köcher"},{"first_name":"Matthias","full_name":"Wilm, Matthias","last_name":"Wilm"},{"last_name":"Allen","full_name":"Allen, Terry","first_name":"Terry"},{"last_name":"Mattaj","full_name":"Mattaj, Iain W.","first_name":"Iain W."},{"last_name":"Doye","first_name":"Valérie","full_name":"Doye, Valérie"}],"doi":"10.1016/s0092-8674(03)00235-6","month":"04","date_created":"2022-04-07T07:57:10Z","citation":{"ama":"Walther TC, Alves A, Pickersgill H, et al. The conserved Nup107-160 complex is critical for nuclear pore complex assembly. <i>Cell</i>. 2003;113(2):195-206. doi:<a href=\"https://doi.org/10.1016/s0092-8674(03)00235-6\">10.1016/s0092-8674(03)00235-6</a>","mla":"Walther, Tobias C., et al. “The Conserved Nup107-160 Complex Is Critical for Nuclear Pore Complex Assembly.” <i>Cell</i>, vol. 113, no. 2, Elsevier, 2003, pp. 195–206, doi:<a href=\"https://doi.org/10.1016/s0092-8674(03)00235-6\">10.1016/s0092-8674(03)00235-6</a>.","short":"T.C. Walther, A. Alves, H. Pickersgill, I. Loı̈odice, M. Hetzer, V. Galy, B.B. Hülsmann, T. Köcher, M. Wilm, T. Allen, I.W. Mattaj, V. Doye, Cell 113 (2003) 195–206.","ieee":"T. C. Walther <i>et al.</i>, “The conserved Nup107-160 complex is critical for nuclear pore complex assembly,” <i>Cell</i>, vol. 113, no. 2. Elsevier, pp. 195–206, 2003.","ista":"Walther TC, Alves A, Pickersgill H, Loı̈odice I, Hetzer M, Galy V, Hülsmann BB, Köcher T, Wilm M, Allen T, Mattaj IW, Doye V. 2003. The conserved Nup107-160 complex is critical for nuclear pore complex assembly. Cell. 113(2), 195–206.","chicago":"Walther, Tobias C., Annabelle Alves, Helen Pickersgill, Isabelle Loı̈odice, Martin Hetzer, Vincent Galy, Bastian B. Hülsmann, et al. “The Conserved Nup107-160 Complex Is Critical for Nuclear Pore Complex Assembly.” <i>Cell</i>. Elsevier, 2003. <a href=\"https://doi.org/10.1016/s0092-8674(03)00235-6\">https://doi.org/10.1016/s0092-8674(03)00235-6</a>.","apa":"Walther, T. C., Alves, A., Pickersgill, H., Loı̈odice, I., Hetzer, M., Galy, V., … Doye, V. (2003). The conserved Nup107-160 complex is critical for nuclear pore complex assembly. <i>Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/s0092-8674(03)00235-6\">https://doi.org/10.1016/s0092-8674(03)00235-6</a>"},"article_processing_charge":"No","user_id":"72615eeb-f1f3-11ec-aa25-d4573ddc34fd","external_id":{"pmid":["12705868"]},"status":"public","page":"195-206"}