{"extern":"1","type":"journal_article","date_updated":"2022-07-18T08:56:36Z","volume":3,"publication_identifier":{"issn":["1932-6203"]},"_id":"11114","author":[{"full_name":"Schulte, Roberta","first_name":"Roberta","last_name":"Schulte"},{"last_name":"Talamas","full_name":"Talamas, Jessica","first_name":"Jessica"},{"full_name":"Doucet, Christine","first_name":"Christine","last_name":"Doucet"},{"last_name":"HETZER","id":"86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed","full_name":"HETZER, Martin W","first_name":"Martin W","orcid":"0000-0002-2111-992X"}],"doi":"10.1371/journal.pone.0002061","abstract":[{"text":"We present a miniaturized pull-down method for the detection of protein-protein interactions using standard affinity chromatography reagents. Binding events between different proteins, which are color-coded with quantum dots (QDs), are visualized on single affinity chromatography beads by fluorescence microscopy. The use of QDs for single molecule detection allows the simultaneous analysis of multiple protein-protein binding events and reduces the amount of time and material needed to perform a pull-down experiment.","lang":"eng"}],"article_number":"e2061","publication_status":"published","publication":"PLoS ONE","article_processing_charge":"No","month":"04","date_created":"2022-04-07T07:55:57Z","citation":{"ieee":"R. Schulte, J. Talamas, C. Doucet, and M. Hetzer, “Single bead affinity detection (SINBAD) for the analysis of protein-protein interactions,” <i>PLoS ONE</i>, vol. 3, no. 4. Public Library of Science, 2008.","short":"R. Schulte, J. Talamas, C. Doucet, M. Hetzer, PLoS ONE 3 (2008).","ama":"Schulte R, Talamas J, Doucet C, Hetzer M. Single bead affinity detection (SINBAD) for the analysis of protein-protein interactions. <i>PLoS ONE</i>. 2008;3(4). doi:<a href=\"https://doi.org/10.1371/journal.pone.0002061\">10.1371/journal.pone.0002061</a>","mla":"Schulte, Roberta, et al. “Single Bead Affinity Detection (SINBAD) for the Analysis of Protein-Protein Interactions.” <i>PLoS ONE</i>, vol. 3, no. 4, e2061, Public Library of Science, 2008, doi:<a href=\"https://doi.org/10.1371/journal.pone.0002061\">10.1371/journal.pone.0002061</a>.","apa":"Schulte, R., Talamas, J., Doucet, C., &#38; Hetzer, M. (2008). Single bead affinity detection (SINBAD) for the analysis of protein-protein interactions. <i>PLoS ONE</i>. Public Library of Science. <a href=\"https://doi.org/10.1371/journal.pone.0002061\">https://doi.org/10.1371/journal.pone.0002061</a>","chicago":"Schulte, Roberta, Jessica Talamas, Christine Doucet, and Martin Hetzer. “Single Bead Affinity Detection (SINBAD) for the Analysis of Protein-Protein Interactions.” <i>PLoS ONE</i>. Public Library of Science, 2008. <a href=\"https://doi.org/10.1371/journal.pone.0002061\">https://doi.org/10.1371/journal.pone.0002061</a>.","ista":"Schulte R, Talamas J, Doucet C, Hetzer M. 2008. Single bead affinity detection (SINBAD) for the analysis of protein-protein interactions. PLoS ONE. 3(4), e2061."},"external_id":{"pmid":["18446240"]},"status":"public","oa":1,"user_id":"72615eeb-f1f3-11ec-aa25-d4573ddc34fd","language":[{"iso":"eng"}],"intvolume":"         3","scopus_import":"1","quality_controlled":"1","title":"Single bead affinity detection (SINBAD) for the analysis of protein-protein interactions","main_file_link":[{"open_access":"1","url":" https://doi.org/10.1371/journal.pone.0002061"}],"oa_version":"Published Version","issue":"4","keyword":["Multidisciplinary"],"pmid":1,"day":"30","article_type":"original","publisher":"Public Library of Science","year":"2008","date_published":"2008-04-30T00:00:00Z"}