{"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1083/jcb.201603053"}],"scopus_import":"1","quality_controlled":"1","title":"Nuclear envelope rupture is induced by actin-based nucleus confinement","issue":"1","oa_version":"Published Version","intvolume":"       215","language":[{"iso":"eng"}],"publisher":"Rockefeller University Press","year":"2016","article_type":"original","day":"03","date_published":"2016-10-03T00:00:00Z","pmid":1,"keyword":["Cell Biology"],"_id":"11069","doi":"10.1083/jcb.201603053","author":[{"last_name":"Hatch","first_name":"Emily M.","full_name":"Hatch, Emily M."},{"id":"86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed","last_name":"HETZER","orcid":"0000-0002-2111-992X","full_name":"HETZER, Martin W","first_name":"Martin W"}],"publication":"Journal of Cell Biology","publication_status":"published","abstract":[{"text":"Repeated rounds of nuclear envelope (NE) rupture and repair have been observed in laminopathy and cancer cells and result in intermittent loss of nucleus compartmentalization. Currently, the causes of NE rupture are unclear. Here, we show that NE rupture in cancer cells relies on the assembly of contractile actin bundles that interact with the nucleus via the linker of nucleoskeleton and cytoskeleton (LINC) complex. We found that the loss of actin bundles or the LINC complex did not rescue nuclear lamina defects, a previously identified determinant of nuclear membrane stability, but did decrease the number and size of chromatin hernias. Finally, NE rupture inhibition could be rescued in cells treated with actin-depolymerizing drugs by mechanically constraining nucleus height. These data suggest a model of NE rupture where weak membrane areas, caused by defects in lamina organization, rupture because of an increase in intranuclear pressure from actin-based nucleus confinement.","lang":"eng"}],"date_updated":"2022-07-18T08:33:47Z","volume":215,"extern":"1","type":"journal_article","publication_identifier":{"issn":["0021-9525","1540-8140"]},"status":"public","page":"27-36","external_id":{"pmid":["27697922"]},"user_id":"72615eeb-f1f3-11ec-aa25-d4573ddc34fd","oa":1,"article_processing_charge":"No","citation":{"ama":"Hatch EM, Hetzer M. Nuclear envelope rupture is induced by actin-based nucleus confinement. <i>Journal of Cell Biology</i>. 2016;215(1):27-36. doi:<a href=\"https://doi.org/10.1083/jcb.201603053\">10.1083/jcb.201603053</a>","mla":"Hatch, Emily M., and Martin Hetzer. “Nuclear Envelope Rupture Is Induced by Actin-Based Nucleus Confinement.” <i>Journal of Cell Biology</i>, vol. 215, no. 1, Rockefeller University Press, 2016, pp. 27–36, doi:<a href=\"https://doi.org/10.1083/jcb.201603053\">10.1083/jcb.201603053</a>.","short":"E.M. Hatch, M. Hetzer, Journal of Cell Biology 215 (2016) 27–36.","ieee":"E. M. Hatch and M. Hetzer, “Nuclear envelope rupture is induced by actin-based nucleus confinement,” <i>Journal of Cell Biology</i>, vol. 215, no. 1. Rockefeller University Press, pp. 27–36, 2016.","chicago":"Hatch, Emily M., and Martin Hetzer. “Nuclear Envelope Rupture Is Induced by Actin-Based Nucleus Confinement.” <i>Journal of Cell Biology</i>. Rockefeller University Press, 2016. <a href=\"https://doi.org/10.1083/jcb.201603053\">https://doi.org/10.1083/jcb.201603053</a>.","ista":"Hatch EM, Hetzer M. 2016. Nuclear envelope rupture is induced by actin-based nucleus confinement. Journal of Cell Biology. 215(1), 27–36.","apa":"Hatch, E. M., &#38; Hetzer, M. (2016). Nuclear envelope rupture is induced by actin-based nucleus confinement. <i>Journal of Cell Biology</i>. Rockefeller University Press. <a href=\"https://doi.org/10.1083/jcb.201603053\">https://doi.org/10.1083/jcb.201603053</a>"},"month":"10","date_created":"2022-04-07T07:47:42Z"}