{"issue":"8","date_published":"2022-04-18T00:00:00Z","publication_status":"published","main_file_link":[{"open_access":"1","url":"https://biblio.ugent.be/publication/8738721"}],"abstract":[{"text":"Much of what we know about the role of auxin in plant development derives from exogenous manipulations of auxin distribution and signaling, using inhibitors, auxins and auxin analogs. In this context, synthetic auxin analogs, such as 1-Naphtalene Acetic Acid (1-NAA), are often favored over the endogenous auxin indole-3-acetic acid (IAA), in part due to their higher stability. While such auxin analogs have proven to be instrumental to reveal the various faces of auxin, they display in some cases distinct bioactivities compared to IAA. Here, we focused on the effect of auxin analogs on the accumulation of PIN proteins in Brefeldin A-sensitive endosomal aggregations (BFA bodies), and the correlation with the ability to elicit Ca 2+ responses. For a set of commonly used auxin analogs, we evaluated if auxin-analog induced Ca 2+ signaling inhibits PIN accumulation. Not all auxin analogs elicited a Ca 2+ response, and their differential ability to elicit Ca 2+ responses correlated partially with their ability to inhibit BFA-body formation. However, in tir1/afb and cngc14, 1-NAA-induced Ca 2+ signaling was strongly impaired, yet 1-NAA still could inhibit PIN accumulation in BFA bodies. This demonstrates that TIR1/AFB-CNGC14-dependent Ca 2+ signaling does not inhibit BFA body formation in Arabidopsis roots.","lang":"eng"}],"department":[{"_id":"JiFr"}],"day":"18","type":"journal_article","date_updated":"2023-08-02T14:07:58Z","pmid":1,"article_processing_charge":"No","month":"04","status":"public","quality_controlled":"1","title":"Auxin analog-induced Ca2+ signaling is independent of inhibition of endosomal aggregation in Arabidopsis roots","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","date_created":"2022-02-03T09:19:01Z","oa":1,"oa_version":"Submitted Version","scopus_import":"1","publisher":"Oxford Academic","ec_funded":1,"article_type":"original","citation":{"short":"R. Wang, E. Himschoot, M. Grenzi, J. Chen, A. Safi, M. Krebs, K. Schumacher, M. Nowack, W. Moeder, K. Yoshioka, D. Van Damme, I. De Smet, D. Geelen, T. Beeckman, J. Friml, A. Costa, S. Vanneste, Journal of Experimental Botany 73 (2022).","ista":"Wang R, Himschoot E, Grenzi M, Chen J, Safi A, Krebs M, Schumacher K, Nowack M, Moeder W, Yoshioka K, Van Damme D, De Smet I, Geelen D, Beeckman T, Friml J, Costa A, Vanneste S. 2022. Auxin analog-induced Ca2+ signaling is independent of inhibition of endosomal aggregation in Arabidopsis roots. Journal of Experimental Botany. 73(8), erac019.","chicago":"Wang, R, E Himschoot, M Grenzi, J Chen, A Safi, M Krebs, K Schumacher, et al. “Auxin Analog-Induced Ca2+ Signaling Is Independent of Inhibition of Endosomal Aggregation in Arabidopsis Roots.” <i>Journal of Experimental Botany</i>. Oxford Academic, 2022. <a href=\"https://doi.org/10.1093/jxb/erac019\">https://doi.org/10.1093/jxb/erac019</a>.","apa":"Wang, R., Himschoot, E., Grenzi, M., Chen, J., Safi, A., Krebs, M., … Vanneste, S. (2022). Auxin analog-induced Ca2+ signaling is independent of inhibition of endosomal aggregation in Arabidopsis roots. <i>Journal of Experimental Botany</i>. Oxford Academic. <a href=\"https://doi.org/10.1093/jxb/erac019\">https://doi.org/10.1093/jxb/erac019</a>","ama":"Wang R, Himschoot E, Grenzi M, et al. Auxin analog-induced Ca2+ signaling is independent of inhibition of endosomal aggregation in Arabidopsis roots. <i>Journal of Experimental Botany</i>. 2022;73(8). doi:<a href=\"https://doi.org/10.1093/jxb/erac019\">10.1093/jxb/erac019</a>","ieee":"R. Wang <i>et al.</i>, “Auxin analog-induced Ca2+ signaling is independent of inhibition of endosomal aggregation in Arabidopsis roots,” <i>Journal of Experimental Botany</i>, vol. 73, no. 8. Oxford Academic, 2022.","mla":"Wang, R., et al. “Auxin Analog-Induced Ca2+ Signaling Is Independent of Inhibition of Endosomal Aggregation in Arabidopsis Roots.” <i>Journal of Experimental Botany</i>, vol. 73, no. 8, erac019, Oxford Academic, 2022, doi:<a href=\"https://doi.org/10.1093/jxb/erac019\">10.1093/jxb/erac019</a>."},"language":[{"iso":"eng"}],"article_number":"erac019","_id":"10717","doi":"10.1093/jxb/erac019","author":[{"first_name":"R","last_name":"Wang","full_name":"Wang, R"},{"full_name":"Himschoot, E","first_name":"E","last_name":"Himschoot"},{"full_name":"Grenzi, M","first_name":"M","last_name":"Grenzi"},{"full_name":"Chen, J","first_name":"J","last_name":"Chen"},{"first_name":"A","last_name":"Safi","full_name":"Safi, A"},{"last_name":"Krebs","first_name":"M","full_name":"Krebs, M"},{"last_name":"Schumacher","first_name":"K","full_name":"Schumacher, K"},{"full_name":"Nowack, MK","first_name":"MK","last_name":"Nowack"},{"full_name":"Moeder, W","last_name":"Moeder","first_name":"W"},{"first_name":"K","last_name":"Yoshioka","full_name":"Yoshioka, K"},{"last_name":"Van Damme","first_name":"D","full_name":"Van Damme, D"},{"full_name":"De Smet, I","first_name":"I","last_name":"De Smet"},{"first_name":"D","last_name":"Geelen","full_name":"Geelen, D"},{"first_name":"T","last_name":"Beeckman","full_name":"Beeckman, T"},{"full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","last_name":"Friml","orcid":"0000-0002-8302-7596"},{"full_name":"Costa, A","last_name":"Costa","first_name":"A"},{"last_name":"Vanneste","first_name":"S","full_name":"Vanneste, S"}],"publication":"Journal of Experimental Botany","publication_identifier":{"eissn":["1460-2431"],"issn":["0022-0957"]},"external_id":{"pmid":["35085386"],"isi":["000764220900001"]},"acknowledgement":"We thank Joerg Kudla (WWU Munster, Germany), Petra Dietrich (F.A. University of Erlangen-Nurnberg, Germany) for sharing published materials, and NASC for providing seeds. We thank Veronique Storme for help with the statistical analyses. Part of the imaging analysis was carried out at NOLIMITS, an advanced imaging facility established by the University of Milan.\r\nThis work was supported by grants of the China Scholarship Council (CSC) to RW and JC; Fonds Wetenschappelijk Onderzoek (FWO) to TB and (G002220N) SV; the special research fund of Ghent University to EH; the Deutsche Forschungsgemeinschaft (DFG) through Grants within FOR964 (MK and KS); Piano di Sviluppo di Ateneo 2019 (University of Milan) to AC; the European Research Council (ERC) T-Rex project 682436 to DVD; the ERC ETAP project 742985 to JF, and by a PhD fellowship from the University of Milan to MG.","isi":1,"project":[{"name":"Tracing Evolution of Auxin Transport and Polarity in Plants","_id":"261099A6-B435-11E9-9278-68D0E5697425","grant_number":"742985","call_identifier":"H2020"}],"volume":73,"intvolume":"        73","year":"2022"}