{"issue":"11","oa_version":"Published Version","scopus_import":"1","quality_controlled":"1","title":"Acute and chronic effects of a light-activated FGF receptor in keratinocytes in vitro and in mice","file":[{"file_size":2055981,"content_type":"application/pdf","creator":"cchlebak","file_id":"10152","date_updated":"2021-10-18T14:48:06Z","date_created":"2021-10-18T14:48:06Z","checksum":"89fb95b211dbe8678809e7cca4626952","access_level":"open_access","success":1,"file_name":"2021_LifeScAlliance_Rauschendorfer.pdf","relation":"main_file"}],"intvolume":"         4","language":[{"iso":"eng"}],"date_published":"2021-09-21T00:00:00Z","year":"2021","article_type":"original","publisher":"Life Science Alliance","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"day":"21","pmid":1,"publication":"Life Science Alliance","publication_status":"published","abstract":[{"text":"FGFs and their high-affinity receptors (FGFRs) play key roles in development, tissue repair, and disease. Because FGFRs bind overlapping sets of ligands, their individual functions cannot be determined using ligand stimulation. Here, we generated a light-activated FGFR2 variant (OptoR2) to selectively activate signaling by the major FGFR in keratinocytes. Illumination of OptoR2-expressing HEK 293T cells activated FGFR signaling with remarkable temporal precision and promoted cell migration and proliferation. In murine and human keratinocytes, OptoR2 activation rapidly induced the classical FGFR signaling pathways and expression of FGF target genes. Surprisingly, multi-level counter-regulation occurred in keratinocytes in vitro and in transgenic mice in vivo, including OptoR2 down-regulation and loss of responsiveness to light activation. These results demonstrate unexpected cell type-specific limitations of optogenetic FGFRs in long-term in vitro and in vivo settings and highlight the complex consequences of transferring optogenetic cell signaling tools into their relevant cellular contexts.","lang":"eng"}],"article_number":"e202101100","_id":"10144","doi":"10.26508/lsa.202101100","author":[{"last_name":"Rauschendorfer","full_name":"Rauschendorfer, Theresa","first_name":"Theresa"},{"last_name":"Gurri","first_name":"Selina","full_name":"Gurri, Selina"},{"full_name":"Heggli, Irina","first_name":"Irina","last_name":"Heggli"},{"last_name":"Maddaluno","full_name":"Maddaluno, Luigi","first_name":"Luigi"},{"first_name":"Michael","full_name":"Meyer, Michael","last_name":"Meyer"},{"last_name":"Inglés Prieto","id":"2A9DB292-F248-11E8-B48F-1D18A9856A87","first_name":"Álvaro","full_name":"Inglés Prieto, Álvaro","orcid":"0000-0002-5409-8571"},{"last_name":"Janovjak","id":"33BA6C30-F248-11E8-B48F-1D18A9856A87","first_name":"Harald L","full_name":"Janovjak, Harald L","orcid":"0000-0002-8023-9315"},{"full_name":"Werner, Sabine","first_name":"Sabine","last_name":"Werner"}],"publication_identifier":{"eissn":["2575-1077"]},"date_updated":"2022-08-31T14:01:56Z","volume":4,"extern":"1","type":"journal_article","has_accepted_license":"1","ddc":["576"],"user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","oa":1,"file_date_updated":"2021-10-18T14:48:06Z","status":"public","external_id":{"pmid":["34548382"]},"citation":{"apa":"Rauschendorfer, T., Gurri, S., Heggli, I., Maddaluno, L., Meyer, M., Inglés Prieto, Á., … Werner, S. (2021). Acute and chronic effects of a light-activated FGF receptor in keratinocytes in vitro and in mice. <i>Life Science Alliance</i>. Life Science Alliance. <a href=\"https://doi.org/10.26508/lsa.202101100\">https://doi.org/10.26508/lsa.202101100</a>","ista":"Rauschendorfer T, Gurri S, Heggli I, Maddaluno L, Meyer M, Inglés Prieto Á, Janovjak HL, Werner S. 2021. Acute and chronic effects of a light-activated FGF receptor in keratinocytes in vitro and in mice. Life Science Alliance. 4(11), e202101100.","chicago":"Rauschendorfer, Theresa, Selina Gurri, Irina Heggli, Luigi Maddaluno, Michael Meyer, Álvaro Inglés Prieto, Harald L Janovjak, and Sabine Werner. “Acute and Chronic Effects of a Light-Activated FGF Receptor in Keratinocytes in Vitro and in Mice.” <i>Life Science Alliance</i>. Life Science Alliance, 2021. <a href=\"https://doi.org/10.26508/lsa.202101100\">https://doi.org/10.26508/lsa.202101100</a>.","ieee":"T. Rauschendorfer <i>et al.</i>, “Acute and chronic effects of a light-activated FGF receptor in keratinocytes in vitro and in mice,” <i>Life Science Alliance</i>, vol. 4, no. 11. Life Science Alliance, 2021.","short":"T. Rauschendorfer, S. Gurri, I. Heggli, L. Maddaluno, M. Meyer, Á. Inglés Prieto, H.L. Janovjak, S. Werner, Life Science Alliance 4 (2021).","ama":"Rauschendorfer T, Gurri S, Heggli I, et al. Acute and chronic effects of a light-activated FGF receptor in keratinocytes in vitro and in mice. <i>Life Science Alliance</i>. 2021;4(11). doi:<a href=\"https://doi.org/10.26508/lsa.202101100\">10.26508/lsa.202101100</a>","mla":"Rauschendorfer, Theresa, et al. “Acute and Chronic Effects of a Light-Activated FGF Receptor in Keratinocytes in Vitro and in Mice.” <i>Life Science Alliance</i>, vol. 4, no. 11, e202101100, Life Science Alliance, 2021, doi:<a href=\"https://doi.org/10.26508/lsa.202101100\">10.26508/lsa.202101100</a>."},"month":"09","date_created":"2021-10-17T22:01:16Z","acknowledgement":"We thank Connor Richterich and Patricia Reinert, ETH Zurich, for invaluable experimental help; Manuela Pérez Berlanga, University Zurich, for help with the confocal imaging; Lukas Fischer for help with electrical engineering; Thomas Hennek, Sol Taguinod, and Dr. Stephan Sonntag, EPIC Phenomics Center, ETH Zürich, for the generation and maintenance of K14-OptoR2 mice; and Dr. Petra Boukamp, Leibniz Institute, Düsseldorf, Germany, for early-passage HaCaT keratinocytes. This work was supported by the ETH Zurich (grant ETH-06 15-1 to S Werner and L Maddaluno), the Swiss National Science Foundation (grant 31003B-189364 to S Werner), and a Marie Curie postdoctoral fellowship from the European Union (to L Maddaluno).","article_processing_charge":"Yes"}