---
_id: '2855'
abstract:
- lang: eng
text: Genomic imprinting leads to preferred expression of either the maternal or
paternal alleles of a subset of genes. Imprinting is essential for mammalian development,
and its deregulation causes many diseases. However, the functional relevance of
imprinting at the cellular level is poorly understood for most imprinted genes.
We used mosaic analysis with double markers (MADM) in mice to create uniparental
disomies (UPDs) and to visualize imprinting effects with single-cell resolution.
Although chromosome 12 UPD did not produce detectable phenotypes, chromosome 7
UPD caused highly significant paternal growth dominance in the liver and lung,
but not in the brain or heart. A single gene on chromosome 7, encoding the secreted
insulin-like growth factor 2 (IGF2), accounts for most of the paternal dominance
effect. Mosaic analyses implied additional imprinted loci on chromosome 7 acting
cell autonomously to transmit the IGF2 signal. Our study reveals chromosome- and
cell-type specificity of genomic imprinting effects.
author:
- first_name: Simon
full_name: Hippenmeyer, Simon
id: 37B36620-F248-11E8-B48F-1D18A9856A87
last_name: Hippenmeyer
orcid: 0000-0003-2279-1061
- first_name: Randy
full_name: Johnson, Randy
last_name: Johnson
- first_name: Liqun
full_name: Luo, Liqun
last_name: Luo
citation:
ama: Hippenmeyer S, Johnson R, Luo L. Mosaic analysis with double markers reveals
cell type specific paternal growth dominance. Cell Reports. 2013;3(3):960-967.
doi:10.1016/j.celrep.2013.02.002
apa: Hippenmeyer, S., Johnson, R., & Luo, L. (2013). Mosaic analysis with double
markers reveals cell type specific paternal growth dominance. Cell Reports.
Cell Press. https://doi.org/10.1016/j.celrep.2013.02.002
chicago: Hippenmeyer, Simon, Randy Johnson, and Liqun Luo. “Mosaic Analysis with
Double Markers Reveals Cell Type Specific Paternal Growth Dominance.” Cell
Reports. Cell Press, 2013. https://doi.org/10.1016/j.celrep.2013.02.002.
ieee: S. Hippenmeyer, R. Johnson, and L. Luo, “Mosaic analysis with double markers
reveals cell type specific paternal growth dominance,” Cell Reports, vol.
3, no. 3. Cell Press, pp. 960–967, 2013.
ista: Hippenmeyer S, Johnson R, Luo L. 2013. Mosaic analysis with double markers
reveals cell type specific paternal growth dominance. Cell Reports. 3(3), 960–967.
mla: Hippenmeyer, Simon, et al. “Mosaic Analysis with Double Markers Reveals Cell
Type Specific Paternal Growth Dominance.” Cell Reports, vol. 3, no. 3,
Cell Press, 2013, pp. 960–67, doi:10.1016/j.celrep.2013.02.002.
short: S. Hippenmeyer, R. Johnson, L. Luo, Cell Reports 3 (2013) 960–967.
date_created: 2018-12-11T11:59:57Z
date_published: 2013-03-28T00:00:00Z
date_updated: 2021-01-12T07:00:16Z
day: '28'
ddc:
- '570'
department:
- _id: SiHi
doi: 10.1016/j.celrep.2013.02.002
file:
- access_level: open_access
checksum: 6e977b918e81384cd571ec5a9d812289
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:17:20Z
date_updated: 2020-07-14T12:45:51Z
file_id: '5274'
file_name: IST-2016-405-v1+1_1-s2.0-S2211124713000612-main.pdf
file_size: 1907211
relation: main_file
file_date_updated: 2020-07-14T12:45:51Z
has_accepted_license: '1'
intvolume: ' 3'
issue: '3'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
page: 960 - 967
publication: Cell Reports
publication_status: published
publisher: Cell Press
publist_id: '3937'
pubrep_id: '405'
quality_controlled: '1'
scopus_import: 1
status: public
title: Mosaic analysis with double markers reveals cell type specific paternal growth
dominance
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 3
year: '2013'
...
---
_id: '2264'
abstract:
- lang: eng
text: Faithful progression through the cell cycle is crucial to the maintenance
and developmental potential of stem cells. Here, we demonstrate that neural stem
cells (NSCs) and intermediate neural progenitor cells (NPCs) employ a zinc-finger
transcription factor specificity protein 2 (Sp2) as a cell cycle regulator in
two temporally and spatially distinct progenitor domains. Differential conditional
deletion of Sp2 in early embryonic cerebral cortical progenitors, and perinatal
olfactory bulb progenitors disrupted transitions through G1, G2 and M phases,
whereas DNA synthesis appeared intact. Cell-autonomous function of Sp2 was identified
by deletion of Sp2 using mosaic analysis with double markers, which clearly established
that conditional Sp2-null NSCs and NPCs are M phase arrested in vivo. Importantly,
conditional deletion of Sp2 led to a decline in the generation of NPCs and neurons
in the developing and postnatal brains. Our findings implicate Sp2-dependent mechanisms
as novel regulators of cell cycle progression, the absence of which disrupts neurogenesis
in the embryonic and postnatal brain.
article_processing_charge: No
author:
- first_name: Huixuan
full_name: Liang, Huixuan
last_name: Liang
- first_name: Guanxi
full_name: Xiao, Guanxi
last_name: Xiao
- first_name: Haifeng
full_name: Yin, Haifeng
last_name: Yin
- first_name: Simon
full_name: Hippenmeyer, Simon
id: 37B36620-F248-11E8-B48F-1D18A9856A87
last_name: Hippenmeyer
orcid: 0000-0003-2279-1061
- first_name: Jonathan
full_name: Horowitz, Jonathan
last_name: Horowitz
- first_name: Troy
full_name: Ghashghaei, Troy
last_name: Ghashghaei
citation:
ama: Liang H, Xiao G, Yin H, Hippenmeyer S, Horowitz J, Ghashghaei T. Neural development
is dependent on the function of specificity protein 2 in cell cycle progression.
Development. 2013;140(3):552-561. doi:10.1242/dev.085621
apa: Liang, H., Xiao, G., Yin, H., Hippenmeyer, S., Horowitz, J., & Ghashghaei,
T. (2013). Neural development is dependent on the function of specificity protein
2 in cell cycle progression. Development. Company of Biologists. https://doi.org/10.1242/dev.085621
chicago: Liang, Huixuan, Guanxi Xiao, Haifeng Yin, Simon Hippenmeyer, Jonathan Horowitz,
and Troy Ghashghaei. “Neural Development Is Dependent on the Function of Specificity
Protein 2 in Cell Cycle Progression.” Development. Company of Biologists,
2013. https://doi.org/10.1242/dev.085621.
ieee: H. Liang, G. Xiao, H. Yin, S. Hippenmeyer, J. Horowitz, and T. Ghashghaei,
“Neural development is dependent on the function of specificity protein 2 in cell
cycle progression,” Development, vol. 140, no. 3. Company of Biologists,
pp. 552–561, 2013.
ista: Liang H, Xiao G, Yin H, Hippenmeyer S, Horowitz J, Ghashghaei T. 2013. Neural
development is dependent on the function of specificity protein 2 in cell cycle
progression. Development. 140(3), 552–561.
mla: Liang, Huixuan, et al. “Neural Development Is Dependent on the Function of
Specificity Protein 2 in Cell Cycle Progression.” Development, vol. 140,
no. 3, Company of Biologists, 2013, pp. 552–61, doi:10.1242/dev.085621.
short: H. Liang, G. Xiao, H. Yin, S. Hippenmeyer, J. Horowitz, T. Ghashghaei, Development
140 (2013) 552–561.
date_created: 2018-12-11T11:56:39Z
date_published: 2013-02-01T00:00:00Z
date_updated: 2021-01-12T06:56:23Z
day: '01'
department:
- _id: SiHi
doi: 10.1242/dev.085621
external_id:
pmid:
- '23293287'
intvolume: ' 140'
issue: '3'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3561788/
month: '02'
oa: 1
oa_version: Submitted Version
page: 552 - 561
pmid: 1
publication: Development
publication_status: published
publisher: Company of Biologists
publist_id: '4681'
quality_controlled: '1'
scopus_import: 1
status: public
title: Neural development is dependent on the function of specificity protein 2 in
cell cycle progression
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 140
year: '2013'
...
---
_id: '2303'
abstract:
- lang: eng
text: MADM (Mosaic Analysis with Double Markers) technology offers a genetic approach
in mice to visualize and concomitantly manipulate genetically defined cells at
clonal level and single cell resolution. MADM employs Cre recombinase/loxP-dependent
interchromosomal mitotic recombination to reconstitute two split marker genes—green
GFP and red tdTomato—and can label sparse clones of homozygous mutant cells in
one color and wild-type cells in the other color in an otherwise unlabeled background.
At present, major MADM applications include lineage tracing, single cell labeling,
conditional knockouts in small populations of cells and induction of uniparental
chromosome disomy to assess effects of genomic imprinting. MADM can be applied
universally in the mouse with the sole limitation being the specificity of the
promoter controlling Cre recombinase expression. Here I review recent developments
and extensions of the MADM technique and give an overview of the major discoveries
and progresses enabled by the implementation of the novel genetic MADM tools.
acknowledgement: This work was supported by IST Austria institutional funds.
article_type: review
author:
- first_name: Simon
full_name: Hippenmeyer, Simon
id: 37B36620-F248-11E8-B48F-1D18A9856A87
last_name: Hippenmeyer
orcid: 0000-0003-2279-1061
citation:
ama: Hippenmeyer S. Dissection of gene function at clonal level using mosaic analysis
with double markers. Frontiers in Biology. 2013;8(6):557-568. doi:10.1007/s11515-013-1279-6
apa: Hippenmeyer, S. (2013). Dissection of gene function at clonal level using mosaic
analysis with double markers. Frontiers in Biology. Springer. https://doi.org/10.1007/s11515-013-1279-6
chicago: Hippenmeyer, Simon. “Dissection of Gene Function at Clonal Level Using
Mosaic Analysis with Double Markers.” Frontiers in Biology. Springer, 2013.
https://doi.org/10.1007/s11515-013-1279-6.
ieee: S. Hippenmeyer, “Dissection of gene function at clonal level using mosaic
analysis with double markers,” Frontiers in Biology, vol. 8, no. 6. Springer,
pp. 557–568, 2013.
ista: Hippenmeyer S. 2013. Dissection of gene function at clonal level using mosaic
analysis with double markers. Frontiers in Biology. 8(6), 557–568.
mla: Hippenmeyer, Simon. “Dissection of Gene Function at Clonal Level Using Mosaic
Analysis with Double Markers.” Frontiers in Biology, vol. 8, no. 6, Springer,
2013, pp. 557–68, doi:10.1007/s11515-013-1279-6.
short: S. Hippenmeyer, Frontiers in Biology 8 (2013) 557–568.
date_created: 2018-12-11T11:56:52Z
date_published: 2013-09-03T00:00:00Z
date_updated: 2021-01-12T06:56:39Z
day: '03'
department:
- _id: SiHi
doi: 10.1007/s11515-013-1279-6
intvolume: ' 8'
issue: '6'
language:
- iso: eng
month: '09'
oa_version: None
page: 557 - 568
publication: Frontiers in Biology
publication_status: published
publisher: Springer
publist_id: '4624'
quality_controlled: '1'
scopus_import: 1
status: public
title: Dissection of gene function at clonal level using mosaic analysis with double
markers
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 8
year: '2013'
...
---
_id: '2263'
abstract:
- lang: eng
text: Nestin-cre transgenic mice have been widely used to direct recombination to
neural stem cells (NSCs) and intermediate neural progenitor cells (NPCs). Here
we report that a readily utilized, and the only commercially available, Nestin-cre
line is insufficient for directing recombination in early embryonic NSCs and NPCs.
Analysis of recombination efficiency in multiple cre-dependent reporters and a
genetic mosaic line revealed consistent temporal and spatial patterns of recombination
in NSCs and NPCs. For comparison we utilized a knock-in Emx1cre line and found
robust recombination in NSCs and NPCs in ventricular and subventricular zones
of the cerebral cortices as early as embryonic day 12.5. In addition we found
that the rate of Nestin-cre driven recombination only reaches sufficiently high
levels in NSCs and NPCs during late embryonic and early postnatal periods. These
findings are important when commercially available cre lines are considered for
directing recombination to embryonic NSCs and NPCs.
author:
- first_name: Huixuan
full_name: Liang, Huixuan
last_name: Liang
- first_name: Simon
full_name: Hippenmeyer, Simon
id: 37B36620-F248-11E8-B48F-1D18A9856A87
last_name: Hippenmeyer
orcid: 0000-0003-2279-1061
- first_name: H.
full_name: Ghashghaei, H.
last_name: Ghashghaei
citation:
ama: Liang H, Hippenmeyer S, Ghashghaei H. A Nestin-cre transgenic mouse is insufficient
for recombination in early embryonic neural progenitors. Biology open.
2012;1(12):1200-1203. doi:10.1242/bio.20122287
apa: Liang, H., Hippenmeyer, S., & Ghashghaei, H. (2012). A Nestin-cre transgenic
mouse is insufficient for recombination in early embryonic neural progenitors.
Biology Open. The Company of Biologists. https://doi.org/10.1242/bio.20122287
chicago: Liang, Huixuan, Simon Hippenmeyer, and H. Ghashghaei. “A Nestin-Cre Transgenic
Mouse Is Insufficient for Recombination in Early Embryonic Neural Progenitors.”
Biology Open. The Company of Biologists, 2012. https://doi.org/10.1242/bio.20122287.
ieee: H. Liang, S. Hippenmeyer, and H. Ghashghaei, “A Nestin-cre transgenic mouse
is insufficient for recombination in early embryonic neural progenitors,” Biology
open, vol. 1, no. 12. The Company of Biologists, pp. 1200–1203, 2012.
ista: Liang H, Hippenmeyer S, Ghashghaei H. 2012. A Nestin-cre transgenic mouse
is insufficient for recombination in early embryonic neural progenitors. Biology
open. 1(12), 1200–1203.
mla: Liang, Huixuan, et al. “A Nestin-Cre Transgenic Mouse Is Insufficient for Recombination
in Early Embryonic Neural Progenitors.” Biology Open, vol. 1, no. 12, The
Company of Biologists, 2012, pp. 1200–03, doi:10.1242/bio.20122287.
short: H. Liang, S. Hippenmeyer, H. Ghashghaei, Biology Open 1 (2012) 1200–1203.
date_created: 2018-12-11T11:56:38Z
date_published: 2012-12-15T00:00:00Z
date_updated: 2021-01-12T06:56:23Z
day: '15'
ddc:
- '576'
department:
- _id: SiHi
doi: 10.1242/bio.20122287
file:
- access_level: open_access
checksum: 605a1800b81227848c361fd6ba7d22ba
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:13:09Z
date_updated: 2020-07-14T12:45:35Z
file_id: '4990'
file_name: IST-2015-387-v1+1_1200.full.pdf
file_size: 726695
relation: main_file
file_date_updated: 2020-07-14T12:45:35Z
has_accepted_license: '1'
intvolume: ' 1'
issue: '12'
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
page: 1200 - 1203
publication: Biology open
publication_status: published
publisher: The Company of Biologists
publist_id: '4682'
pubrep_id: '387'
quality_controlled: '1'
scopus_import: 1
status: public
title: A Nestin-cre transgenic mouse is insufficient for recombination in early embryonic
neural progenitors
tmp:
image: /images/cc_by_nc_sa.png
legal_code_url: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC
BY-NC-SA 4.0)
short: CC BY-NC-SA (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 1
year: '2012'
...