[{"oa_version":"Published Version","doi":"10.1007/s10827-020-00740-x","day":"01","publisher":"Springer Nature","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"department":[{"_id":"SaSi"}],"publication_identifier":{"eissn":["1573-6873"],"issn":["0929-5313"]},"year":"2020","author":[{"orcid":"0000-0003-0002-1867","full_name":"Cubero, Ryan J","last_name":"Cubero","id":"850B2E12-9CD4-11E9-837F-E719E6697425","first_name":"Ryan J"},{"full_name":"Marsili, Matteo","last_name":"Marsili","first_name":"Matteo"},{"full_name":"Roudi, Yasser","last_name":"Roudi","first_name":"Yasser"}],"isi":1,"citation":{"chicago":"Cubero, Ryan J, Matteo Marsili, and Yasser Roudi. “Multiscale Relevance and Informative Encoding in Neuronal Spike Trains.” <i>Journal of Computational Neuroscience</i>. Springer Nature, 2020. <a href=\"https://doi.org/10.1007/s10827-020-00740-x\">https://doi.org/10.1007/s10827-020-00740-x</a>.","ama":"Cubero RJ, Marsili M, Roudi Y. Multiscale relevance and informative encoding in neuronal spike trains. <i>Journal of Computational Neuroscience</i>. 2020;48:85-102. doi:<a href=\"https://doi.org/10.1007/s10827-020-00740-x\">10.1007/s10827-020-00740-x</a>","mla":"Cubero, Ryan J., et al. “Multiscale Relevance and Informative Encoding in Neuronal Spike Trains.” <i>Journal of Computational Neuroscience</i>, vol. 48, Springer Nature, 2020, pp. 85–102, doi:<a href=\"https://doi.org/10.1007/s10827-020-00740-x\">10.1007/s10827-020-00740-x</a>.","ista":"Cubero RJ, Marsili M, Roudi Y. 2020. Multiscale relevance and informative encoding in neuronal spike trains. Journal of Computational Neuroscience. 48, 85–102.","apa":"Cubero, R. J., Marsili, M., &#38; Roudi, Y. (2020). Multiscale relevance and informative encoding in neuronal spike trains. <i>Journal of Computational Neuroscience</i>. Springer Nature. <a href=\"https://doi.org/10.1007/s10827-020-00740-x\">https://doi.org/10.1007/s10827-020-00740-x</a>","short":"R.J. Cubero, M. Marsili, Y. Roudi, Journal of Computational Neuroscience 48 (2020) 85–102.","ieee":"R. J. Cubero, M. Marsili, and Y. Roudi, “Multiscale relevance and informative encoding in neuronal spike trains,” <i>Journal of Computational Neuroscience</i>, vol. 48. Springer Nature, pp. 85–102, 2020."},"type":"journal_article","file":[{"access_level":"open_access","file_name":"10827_2020_740_MOESM1_ESM.pdf","date_updated":"2020-07-14T12:47:56Z","content_type":"application/pdf","file_size":1941355,"checksum":"036e9451d6cd0c190ad25791bf82393b","relation":"supplementary_material","date_created":"2020-01-28T09:31:09Z","creator":"rcubero","file_id":"7380"},{"file_size":3257880,"content_type":"application/pdf","date_updated":"2020-07-14T12:47:56Z","relation":"main_file","checksum":"4dd8b1fd4b54486f79d82ac7b2a412b2","access_level":"open_access","file_name":"Cubero2020_Article_MultiscaleRelevanceAndInformat.pdf","file_id":"7381","creator":"rcubero","date_created":"2020-01-28T09:31:09Z"}],"article_type":"original","ddc":["004","519","570"],"status":"public","has_accepted_license":"1","language":[{"iso":"eng"}],"abstract":[{"text":"Neuronal responses to complex stimuli and tasks can encompass a wide range of time scales. Understanding these responses requires measures that characterize how the information on these response patterns are represented across multiple temporal resolutions. In this paper we propose a metric – which we call multiscale relevance (MSR) – to capture the dynamical variability of the activity of single neurons across different time scales. The MSR is a non-parametric, fully featureless indicator in that it uses only the time stamps of the firing activity without resorting to any a priori covariate or invoking any specific structure in the tuning curve for neural activity. When applied to neural data from the mEC and from the ADn and PoS regions of freely-behaving rodents, we found that neurons having low MSR tend to have low mutual information and low firing sparsity across the correlates that are believed to be encoded by the region of the brain where the recordings were made. In addition, neurons with high MSR contain significant information on spatial navigation and allow to decode spatial position or head direction as efficiently as those neurons whose firing activity has high mutual information with the covariate to be decoded and significantly better than the set of neurons with high local variations in their interspike intervals. Given these results, we propose that the MSR can be used as a measure to rank and select neurons for their information content without the need to appeal to any a priori covariate.","lang":"eng"}],"publication_status":"published","quality_controlled":"1","page":"85-102","publication":"Journal of Computational Neuroscience","file_date_updated":"2020-07-14T12:47:56Z","external_id":{"isi":["000515321800006"]},"date_created":"2020-01-28T10:34:00Z","_id":"7369","project":[{"name":"ISTplus - Postdoctoral Fellowships","grant_number":"754411","_id":"260C2330-B435-11E9-9278-68D0E5697425","call_identifier":"H2020"}],"month":"02","date_updated":"2023-08-17T14:35:22Z","title":"Multiscale relevance and informative encoding in neuronal spike trains","ec_funded":1,"keyword":["Time series analysis","Multiple time scale analysis","Spike train data","Information theory","Bayesian decoding"],"article_processing_charge":"Yes (via OA deal)","date_published":"2020-02-01T00:00:00Z","scopus_import":"1","volume":48,"intvolume":"        48","oa":1,"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","acknowledgement":"This research was supported by the Kavli Foundation and the Centre of Excellence scheme of the Research Council of Norway (Centre for Neural Computation). RJC is currently receiving funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie Grant Agreement No. 754411."},{"scopus_import":"1","volume":10,"oa":1,"intvolume":"        10","issue":"1","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","article_number":"5559","publication":"Scientific reports","file_date_updated":"2020-07-14T12:48:01Z","external_id":{"isi":["000560406800007"]},"date_created":"2020-04-05T22:00:47Z","_id":"7632","month":"03","title":"Action representation in the mouse parieto-frontal network","date_updated":"2023-08-18T10:25:13Z","article_processing_charge":"No","date_published":"2020-03-27T00:00:00Z","citation":{"short":"T. Tombaz, B.A. Dunn, K. Hovde, R.J. Cubero, B. Mimica, P. Mamidanna, Y. Roudi, J.R. Whitlock, Scientific Reports 10 (2020).","apa":"Tombaz, T., Dunn, B. A., Hovde, K., Cubero, R. J., Mimica, B., Mamidanna, P., … Whitlock, J. R. (2020). Action representation in the mouse parieto-frontal network. <i>Scientific Reports</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41598-020-62089-6\">https://doi.org/10.1038/s41598-020-62089-6</a>","ieee":"T. Tombaz <i>et al.</i>, “Action representation in the mouse parieto-frontal network,” <i>Scientific reports</i>, vol. 10, no. 1. Springer Nature, 2020.","ama":"Tombaz T, Dunn BA, Hovde K, et al. Action representation in the mouse parieto-frontal network. <i>Scientific reports</i>. 2020;10(1). doi:<a href=\"https://doi.org/10.1038/s41598-020-62089-6\">10.1038/s41598-020-62089-6</a>","chicago":"Tombaz, Tuce, Benjamin A. Dunn, Karoline Hovde, Ryan J Cubero, Bartul Mimica, Pranav Mamidanna, Yasser Roudi, and Jonathan R. Whitlock. “Action Representation in the Mouse Parieto-Frontal Network.” <i>Scientific Reports</i>. Springer Nature, 2020. <a href=\"https://doi.org/10.1038/s41598-020-62089-6\">https://doi.org/10.1038/s41598-020-62089-6</a>.","mla":"Tombaz, Tuce, et al. “Action Representation in the Mouse Parieto-Frontal Network.” <i>Scientific Reports</i>, vol. 10, no. 1, 5559, Springer Nature, 2020, doi:<a href=\"https://doi.org/10.1038/s41598-020-62089-6\">10.1038/s41598-020-62089-6</a>.","ista":"Tombaz T, Dunn BA, Hovde K, Cubero RJ, Mimica B, Mamidanna P, Roudi Y, Whitlock JR. 2020. Action representation in the mouse parieto-frontal network. Scientific reports. 10(1), 5559."},"type":"journal_article","file":[{"date_created":"2020-04-06T10:44:23Z","creator":"dernst","file_id":"7644","file_name":"2020_ScientificReports_Tombaz.pdf","access_level":"open_access","checksum":"e6cfaaaf7986532132934400038b824a","relation":"main_file","file_size":2621249,"date_updated":"2020-07-14T12:48:01Z","content_type":"application/pdf"}],"article_type":"original","ddc":["570"],"has_accepted_license":"1","status":"public","language":[{"iso":"eng"}],"publication_status":"published","abstract":[{"text":"The posterior parietal cortex (PPC) and frontal motor areas comprise a cortical network supporting goal-directed behaviour, with functions including sensorimotor transformations and decision making. In primates, this network links performed and observed actions via mirror neurons, which fire both when individuals perform an action and when they observe the same action performed by a conspecific. Mirror neurons are believed to be important for social learning, but it is not known whether mirror-like neurons occur in similar networks in other social species, such as rodents, or if they can be measured in such models using paradigms where observers passively view a demonstrator. Therefore, we imaged Ca2+ responses in PPC and secondary motor cortex (M2) while mice performed and observed pellet-reaching and wheel-running tasks, and found that cell populations in both areas robustly encoded several naturalistic behaviours. However, neural responses to the same set of observed actions were absent, although we verified that observer mice were attentive to performers and that PPC neurons responded reliably to visual cues. Statistical modelling also indicated that executed actions outperformed observed actions in predicting neural responses. These results raise the possibility that sensorimotor action recognition in rodents could take place outside of the parieto-frontal circuit, and underscore that detecting socially-driven neural coding depends critically on the species and behavioural paradigm used.","lang":"eng"}],"quality_controlled":"1","oa_version":"Published Version","doi":"10.1038/s41598-020-62089-6","day":"27","publisher":"Springer Nature","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"department":[{"_id":"SaSi"}],"publication_identifier":{"eissn":["20452322"]},"year":"2020","isi":1,"author":[{"last_name":"Tombaz","full_name":"Tombaz, Tuce","first_name":"Tuce"},{"first_name":"Benjamin A.","last_name":"Dunn","full_name":"Dunn, Benjamin A."},{"first_name":"Karoline","last_name":"Hovde","full_name":"Hovde, Karoline"},{"last_name":"Cubero","full_name":"Cubero, Ryan J","orcid":"0000-0003-0002-1867","id":"850B2E12-9CD4-11E9-837F-E719E6697425","first_name":"Ryan J"},{"first_name":"Bartul","last_name":"Mimica","full_name":"Mimica, Bartul"},{"first_name":"Pranav","last_name":"Mamidanna","full_name":"Mamidanna, Pranav"},{"first_name":"Yasser","last_name":"Roudi","full_name":"Roudi, Yasser"},{"full_name":"Whitlock, Jonathan R.","last_name":"Whitlock","first_name":"Jonathan R."}]},{"month":"04","title":"Dopamine transporter trafficking and Rit2 GTPase: Mechanism of action and in vivo impact","date_updated":"2023-08-21T06:26:22Z","article_processing_charge":"No","date_published":"2020-04-17T00:00:00Z","page":"5229-5244","publication":"Journal of Biological Chemistry","external_id":{"isi":["000530288000006"],"pmid":["32132171"]},"date_created":"2020-05-24T22:00:59Z","_id":"7880","main_file_link":[{"open_access":"1","url":"https://escholarship.umassmed.edu/oapubs/4187"}],"volume":295,"oa":1,"intvolume":"       295","issue":"16","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","scopus_import":"1","department":[{"_id":"SaSi"}],"publication_identifier":{"eissn":["1083351X"],"issn":["00219258"]},"year":"2020","author":[{"last_name":"Fagan","full_name":"Fagan, Rita R.","first_name":"Rita R."},{"first_name":"Patrick J.","last_name":"Kearney","full_name":"Kearney, Patrick J."},{"full_name":"Sweeney, Carolyn G.","last_name":"Sweeney","first_name":"Carolyn G."},{"last_name":"Luethi","full_name":"Luethi, Dino","first_name":"Dino"},{"full_name":"Schoot Uiterkamp, Florianne E","last_name":"Schoot Uiterkamp","first_name":"Florianne E","id":"3526230C-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Schicker, Klaus","last_name":"Schicker","first_name":"Klaus"},{"first_name":"Brian S.","full_name":"Alejandro, Brian S.","last_name":"Alejandro"},{"first_name":"Lauren C.","full_name":"O'Connor, Lauren C.","last_name":"O'Connor"},{"first_name":"Harald H.","full_name":"Sitte, Harald H.","last_name":"Sitte"},{"full_name":"Melikian, Haley E.","last_name":"Melikian","first_name":"Haley E."}],"isi":1,"oa_version":"Submitted Version","doi":"10.1074/jbc.RA120.012628","day":"17","publisher":"ASBMB Publications","pmid":1,"status":"public","language":[{"iso":"eng"}],"publication_status":"published","abstract":[{"text":"Following its evoked release, dopamine (DA) signaling is rapidly terminated by presynaptic reuptake, mediated by the cocaine-sensitive DA transporter (DAT). DAT surface availability is dynamically regulated by endocytic trafficking, and direct protein kinase C (PKC) activation acutely diminishes DAT surface expression by accelerating DAT internalization. Previous cell line studies demonstrated that PKC-stimulated DAT endocytosis requires both Ack1 inactivation, which releases a DAT-specific endocytic brake, and the neuronal GTPase, Rit2, which binds DAT. However, it is unknown whether Rit2 is required for PKC-stimulated DAT endocytosis in DAergic terminals or whether there are region- and/or sex-dependent differences in PKC-stimulated DAT trafficking. Moreover, the mechanisms by which Rit2 controls PKC-stimulated DAT endocytosis are unknown. Here, we directly examined these important questions. Ex vivo studies revealed that PKC activation acutely decreased DAT surface expression selectively in ventral, but not dorsal, striatum. AAV-mediated, conditional Rit2 knockdown in DAergic neurons impacted baseline DAT surface:intracellular distribution in DAergic terminals from female ventral, but not dorsal, striatum. Further, Rit2 was required for PKC-stimulated DAT internalization in both male and female ventral striatum. FRET and surface pulldown studies in cell lines revealed that PKC activation drives DAT-Rit2 surface dissociation and that the DAT N terminus is required for both PKC-mediated DAT-Rit2 dissociation and DAT internalization. Finally, we found that Rit2 and Ack1 independently converge on DAT to facilitate PKC-stimulated DAT endocytosis. Together, our data provide greater insight into mechanisms that mediate PKC-regulated DAT internalization and reveal unexpected region-specific differences in PKC-stimulated DAT trafficking in bona fide DAergic terminals. ","lang":"eng"}],"quality_controlled":"1","citation":{"chicago":"Fagan, Rita R., Patrick J. Kearney, Carolyn G. Sweeney, Dino Luethi, Florianne E Schoot Uiterkamp, Klaus Schicker, Brian S. Alejandro, Lauren C. O’Connor, Harald H. Sitte, and Haley E. Melikian. “Dopamine Transporter Trafficking and Rit2 GTPase: Mechanism of Action and in Vivo Impact.” <i>Journal of Biological Chemistry</i>. ASBMB Publications, 2020. <a href=\"https://doi.org/10.1074/jbc.RA120.012628\">https://doi.org/10.1074/jbc.RA120.012628</a>.","ama":"Fagan RR, Kearney PJ, Sweeney CG, et al. Dopamine transporter trafficking and Rit2 GTPase: Mechanism of action and in vivo impact. <i>Journal of Biological Chemistry</i>. 2020;295(16):5229-5244. doi:<a href=\"https://doi.org/10.1074/jbc.RA120.012628\">10.1074/jbc.RA120.012628</a>","mla":"Fagan, Rita R., et al. “Dopamine Transporter Trafficking and Rit2 GTPase: Mechanism of Action and in Vivo Impact.” <i>Journal of Biological Chemistry</i>, vol. 295, no. 16, ASBMB Publications, 2020, pp. 5229–44, doi:<a href=\"https://doi.org/10.1074/jbc.RA120.012628\">10.1074/jbc.RA120.012628</a>.","ista":"Fagan RR, Kearney PJ, Sweeney CG, Luethi D, Schoot Uiterkamp FE, Schicker K, Alejandro BS, O’Connor LC, Sitte HH, Melikian HE. 2020. Dopamine transporter trafficking and Rit2 GTPase: Mechanism of action and in vivo impact. Journal of Biological Chemistry. 295(16), 5229–5244.","short":"R.R. Fagan, P.J. Kearney, C.G. Sweeney, D. Luethi, F.E. Schoot Uiterkamp, K. Schicker, B.S. Alejandro, L.C. O’Connor, H.H. Sitte, H.E. Melikian, Journal of Biological Chemistry 295 (2020) 5229–5244.","apa":"Fagan, R. R., Kearney, P. J., Sweeney, C. G., Luethi, D., Schoot Uiterkamp, F. E., Schicker, K., … Melikian, H. E. (2020). Dopamine transporter trafficking and Rit2 GTPase: Mechanism of action and in vivo impact. <i>Journal of Biological Chemistry</i>. ASBMB Publications. <a href=\"https://doi.org/10.1074/jbc.RA120.012628\">https://doi.org/10.1074/jbc.RA120.012628</a>","ieee":"R. R. Fagan <i>et al.</i>, “Dopamine transporter trafficking and Rit2 GTPase: Mechanism of action and in vivo impact,” <i>Journal of Biological Chemistry</i>, vol. 295, no. 16. ASBMB Publications, pp. 5229–5244, 2020."},"type":"journal_article","article_type":"original"},{"department":[{"_id":"SaSi"}],"publication_identifier":{"eissn":["2045-2322"]},"tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"year":"2019","isi":1,"author":[{"id":"3838F452-F248-11E8-B48F-1D18A9856A87","first_name":"Margaret E","last_name":"Maes","full_name":"Maes, Margaret E","orcid":"0000-0001-9642-1085"},{"first_name":"J. A.","full_name":"Grosser, J. A.","last_name":"Grosser"},{"last_name":"Fehrman","full_name":"Fehrman, R. L.","first_name":"R. L."},{"first_name":"C. L.","last_name":"Schlamp","full_name":"Schlamp, C. L."},{"last_name":"Nickells","full_name":"Nickells, R. W.","first_name":"R. W."}],"doi":"10.1038/s41598-019-53049-w","oa_version":"Published Version","publisher":"Springer Nature","day":"12","publication_status":"published","abstract":[{"text":"BAX, a member of the BCL2 gene family, controls the committed step of the intrinsic apoptotic program. Mitochondrial fragmentation is a commonly observed feature of apoptosis, which occurs through the process of mitochondrial fission. BAX has consistently been associated with mitochondrial fission, yet how BAX participates in the process of mitochondrial fragmentation during apoptosis remains to be tested. Time-lapse imaging of BAX recruitment and mitochondrial fragmentation demonstrates that rapid mitochondrial fragmentation during apoptosis occurs after the complete recruitment of BAX to the mitochondrial outer membrane (MOM). The requirement of a fully functioning BAX protein for the fission process was demonstrated further in BAX/BAK-deficient HCT116 cells expressing a P168A mutant of BAX. The mutant performed fusion to restore the mitochondrial network. but was not demonstrably recruited to the MOM after apoptosis induction. Under these conditions, mitochondrial fragmentation was blocked. Additionally, we show that loss of the fission protein, dynamin-like protein 1 (DRP1), does not temporally affect the initiation time or rate of BAX recruitment, but does reduce the final level of BAX recruited to the MOM during the late phase of BAX recruitment. These correlative observations suggest a model where late-stage BAX oligomers play a functional part of the mitochondrial fragmentation machinery in apoptotic cells.","lang":"eng"}],"has_accepted_license":"1","status":"public","pmid":1,"language":[{"iso":"eng"}],"quality_controlled":"1","file":[{"file_name":"2019_ScientificReports_Maes.pdf","access_level":"open_access","relation":"main_file","checksum":"9ab397ed9c1c454b34bffb8cc863d734","content_type":"application/pdf","date_updated":"2020-07-14T12:47:49Z","file_size":6467393,"date_created":"2019-11-25T07:49:52Z","creator":"dernst","file_id":"7096"}],"citation":{"mla":"Maes, Margaret E., et al. “Completion of BAX Recruitment Correlates with Mitochondrial Fission during Apoptosis.” <i>Scientific Reports</i>, vol. 9, 16565, Springer Nature, 2019, doi:<a href=\"https://doi.org/10.1038/s41598-019-53049-w\">10.1038/s41598-019-53049-w</a>.","ista":"Maes ME, Grosser JA, Fehrman RL, Schlamp CL, Nickells RW. 2019. Completion of BAX recruitment correlates with mitochondrial fission during apoptosis. Scientific Reports. 9, 16565.","chicago":"Maes, Margaret E, J. A. Grosser, R. L. Fehrman, C. L. Schlamp, and R. W. Nickells. “Completion of BAX Recruitment Correlates with Mitochondrial Fission during Apoptosis.” <i>Scientific Reports</i>. Springer Nature, 2019. <a href=\"https://doi.org/10.1038/s41598-019-53049-w\">https://doi.org/10.1038/s41598-019-53049-w</a>.","ama":"Maes ME, Grosser JA, Fehrman RL, Schlamp CL, Nickells RW. Completion of BAX recruitment correlates with mitochondrial fission during apoptosis. <i>Scientific Reports</i>. 2019;9. doi:<a href=\"https://doi.org/10.1038/s41598-019-53049-w\">10.1038/s41598-019-53049-w</a>","ieee":"M. E. Maes, J. A. Grosser, R. L. Fehrman, C. L. Schlamp, and R. W. Nickells, “Completion of BAX recruitment correlates with mitochondrial fission during apoptosis,” <i>Scientific Reports</i>, vol. 9. Springer Nature, 2019.","short":"M.E. Maes, J.A. Grosser, R.L. Fehrman, C.L. Schlamp, R.W. Nickells, Scientific Reports 9 (2019).","apa":"Maes, M. E., Grosser, J. A., Fehrman, R. L., Schlamp, C. L., &#38; Nickells, R. W. (2019). Completion of BAX recruitment correlates with mitochondrial fission during apoptosis. <i>Scientific Reports</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41598-019-53049-w\">https://doi.org/10.1038/s41598-019-53049-w</a>"},"type":"journal_article","ddc":["570"],"article_type":"original","title":"Completion of BAX recruitment correlates with mitochondrial fission during apoptosis","date_updated":"2023-08-30T07:26:54Z","month":"11","article_processing_charge":"No","date_published":"2019-11-12T00:00:00Z","publication":"Scientific Reports","file_date_updated":"2020-07-14T12:47:49Z","_id":"7095","external_id":{"pmid":["31719602"],"isi":["000495857600019"]},"date_created":"2019-11-25T07:45:17Z","volume":9,"article_number":"16565","oa":1,"intvolume":"         9","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","scopus_import":"1"},{"publication_identifier":{"eissn":["20411723"]},"department":[{"_id":"SaSi"}],"tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"year":"2019","isi":1,"author":[{"full_name":"Moussa, Hagar F.","last_name":"Moussa","first_name":"Hagar F."},{"full_name":"Bsteh, Daniel","last_name":"Bsteh","first_name":"Daniel"},{"last_name":"Yelagandula","full_name":"Yelagandula, Ramesh","first_name":"Ramesh"},{"first_name":"Carina","full_name":"Pribitzer, Carina","last_name":"Pribitzer"},{"first_name":"Karin","last_name":"Stecher","full_name":"Stecher, Karin"},{"id":"4D883232-F248-11E8-B48F-1D18A9856A87","first_name":"Katarina","full_name":"Bartalska, Katarina","last_name":"Bartalska"},{"last_name":"Michetti","full_name":"Michetti, Luca","first_name":"Luca"},{"last_name":"Wang","full_name":"Wang, Jingkui","first_name":"Jingkui"},{"last_name":"Zepeda-Martinez","full_name":"Zepeda-Martinez, Jorge A.","first_name":"Jorge A."},{"last_name":"Elling","full_name":"Elling, Ulrich","first_name":"Ulrich"},{"full_name":"Stuckey, Jacob I.","last_name":"Stuckey","first_name":"Jacob I."},{"first_name":"Lindsey I.","full_name":"James, Lindsey I.","last_name":"James"},{"first_name":"Stephen V.","full_name":"Frye, Stephen V.","last_name":"Frye"},{"first_name":"Oliver","last_name":"Bell","full_name":"Bell, Oliver"}],"doi":"10.1038/s41467-019-09628-6","oa_version":"Published Version","publisher":"Springer Nature","day":"29","publication_status":"published","abstract":[{"lang":"eng","text":"Polycomb group (PcG) proteins play critical roles in the epigenetic inheritance of cell fate. The Polycomb Repressive Complexes PRC1 and PRC2 catalyse distinct chromatin modifications to enforce gene silencing, but how transcriptional repression is propagated through mitotic cell divisions remains a key unresolved question. Using reversible tethering of PcG proteins to ectopic sites in mouse embryonic stem cells, here we show that PRC1 can trigger transcriptional repression and Polycomb-dependent chromatin modifications. We find that canonical PRC1 (cPRC1), but not variant PRC1, maintains gene silencing through cell division upon reversal of tethering. Propagation of gene repression is sustained by cis-acting histone modifications, PRC2-mediated H3K27me3 and cPRC1-mediated H2AK119ub1, promoting a sequence-independent feedback mechanism for PcG protein recruitment. Thus, the distinct PRC1 complexes present in vertebrates can differentially regulate epigenetic maintenance of gene silencing, potentially enabling dynamic heritable responses to complex stimuli. Our findings reveal how PcG repression is potentially inherited in vertebrates."}],"status":"public","has_accepted_license":"1","language":[{"iso":"eng"}],"quality_controlled":"1","file":[{"file_id":"6448","date_created":"2019-05-14T08:45:51Z","creator":"dernst","file_size":1223647,"content_type":"application/pdf","date_updated":"2020-07-14T12:47:29Z","checksum":"6550a328335396c856db4cbdda7d2994","relation":"main_file","access_level":"open_access","file_name":"2019_NatureComm_Moussa.pdf"}],"citation":{"ista":"Moussa HF, Bsteh D, Yelagandula R, Pribitzer C, Stecher K, Bartalska K, Michetti L, Wang J, Zepeda-Martinez JA, Elling U, Stuckey JI, James LI, Frye SV, Bell O. 2019. Canonical PRC1 controls sequence-independent propagation of Polycomb-mediated gene silencing. Nature Communications. 10(1), 1931.","mla":"Moussa, Hagar F., et al. “Canonical PRC1 Controls Sequence-Independent Propagation of Polycomb-Mediated Gene Silencing.” <i>Nature Communications</i>, vol. 10, no. 1, 1931, Springer Nature, 2019, doi:<a href=\"https://doi.org/10.1038/s41467-019-09628-6\">10.1038/s41467-019-09628-6</a>.","chicago":"Moussa, Hagar F., Daniel Bsteh, Ramesh Yelagandula, Carina Pribitzer, Karin Stecher, Katarina Bartalska, Luca Michetti, et al. “Canonical PRC1 Controls Sequence-Independent Propagation of Polycomb-Mediated Gene Silencing.” <i>Nature Communications</i>. Springer Nature, 2019. <a href=\"https://doi.org/10.1038/s41467-019-09628-6\">https://doi.org/10.1038/s41467-019-09628-6</a>.","ama":"Moussa HF, Bsteh D, Yelagandula R, et al. Canonical PRC1 controls sequence-independent propagation of Polycomb-mediated gene silencing. <i>Nature Communications</i>. 2019;10(1). doi:<a href=\"https://doi.org/10.1038/s41467-019-09628-6\">10.1038/s41467-019-09628-6</a>","ieee":"H. F. Moussa <i>et al.</i>, “Canonical PRC1 controls sequence-independent propagation of Polycomb-mediated gene silencing,” <i>Nature Communications</i>, vol. 10, no. 1. Springer Nature, 2019.","short":"H.F. Moussa, D. Bsteh, R. Yelagandula, C. Pribitzer, K. Stecher, K. Bartalska, L. Michetti, J. Wang, J.A. Zepeda-Martinez, U. Elling, J.I. Stuckey, L.I. James, S.V. Frye, O. Bell, Nature Communications 10 (2019).","apa":"Moussa, H. F., Bsteh, D., Yelagandula, R., Pribitzer, C., Stecher, K., Bartalska, K., … Bell, O. (2019). Canonical PRC1 controls sequence-independent propagation of Polycomb-mediated gene silencing. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-019-09628-6\">https://doi.org/10.1038/s41467-019-09628-6</a>"},"type":"journal_article","ddc":["570"],"title":"Canonical PRC1 controls sequence-independent propagation of Polycomb-mediated gene silencing","date_updated":"2023-08-25T10:31:56Z","month":"04","article_processing_charge":"No","date_published":"2019-04-29T00:00:00Z","publication":"Nature Communications","file_date_updated":"2020-07-14T12:47:29Z","_id":"6412","external_id":{"isi":["000466118700002"]},"date_created":"2019-05-13T07:58:35Z","volume":10,"article_number":"1931","oa":1,"intvolume":"        10","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","issue":"1","scopus_import":"1"},{"file_date_updated":"2020-07-14T12:47:33Z","publication":"Neuroscience Letters","project":[{"name":"International IST Doctoral Program","grant_number":"665385","_id":"2564DBCA-B435-11E9-9278-68D0E5697425","call_identifier":"H2020"},{"name":"Microglia action towards neuronal circuit formation and function in health and disease","grant_number":"715571","_id":"25D4A630-B435-11E9-9278-68D0E5697425","call_identifier":"H2020"},{"_id":"267F75D8-B435-11E9-9278-68D0E5697425","name":"Modulating microglia through G protein-coupled receptor (GPCR) signaling"}],"_id":"6521","date_created":"2019-06-05T13:16:24Z","external_id":{"pmid":["31158432"],"isi":["000486094600037"]},"title":"Targeting microglia with lentivirus and AAV: Recent advances and remaining challenges","date_updated":"2023-08-28T09:30:57Z","month":"08","date_published":"2019-08-10T00:00:00Z","article_processing_charge":"No","ec_funded":1,"scopus_import":"1","volume":707,"article_number":"134310","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","oa":1,"intvolume":"       707","doi":"10.1016/j.neulet.2019.134310","oa_version":"Published Version","publisher":"Elsevier","day":"10","department":[{"_id":"SaSi"}],"publication_identifier":{"issn":["0304-3940"]},"tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"author":[{"first_name":"Margaret E","id":"3838F452-F248-11E8-B48F-1D18A9856A87","last_name":"Maes","orcid":"0000-0001-9642-1085","full_name":"Maes, Margaret E"},{"id":"3483CF6C-F248-11E8-B48F-1D18A9856A87","first_name":"Gloria","last_name":"Colombo","orcid":"0000-0001-9434-8902","full_name":"Colombo, Gloria"},{"last_name":"Schulz","orcid":"0000-0001-5297-733X","full_name":"Schulz, Rouven","first_name":"Rouven","id":"4C5E7B96-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Siegert, Sandra","orcid":"0000-0001-8635-0877","last_name":"Siegert","id":"36ACD32E-F248-11E8-B48F-1D18A9856A87","first_name":"Sandra"}],"isi":1,"year":"2019","file":[{"creator":"dernst","date_created":"2019-06-08T11:44:20Z","file_id":"6551","access_level":"open_access","file_name":"2019_Neuroscience_Maes.pdf","content_type":"application/pdf","date_updated":"2020-07-14T12:47:33Z","file_size":1779287,"checksum":"553c9dbd39727fbed55ee991c51ca4d1","relation":"main_file"}],"type":"journal_article","citation":{"apa":"Maes, M. E., Colombo, G., Schulz, R., &#38; Siegert, S. (2019). Targeting microglia with lentivirus and AAV: Recent advances and remaining challenges. <i>Neuroscience Letters</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.neulet.2019.134310\">https://doi.org/10.1016/j.neulet.2019.134310</a>","short":"M.E. Maes, G. Colombo, R. Schulz, S. Siegert, Neuroscience Letters 707 (2019).","ieee":"M. E. Maes, G. Colombo, R. Schulz, and S. Siegert, “Targeting microglia with lentivirus and AAV: Recent advances and remaining challenges,” <i>Neuroscience Letters</i>, vol. 707. Elsevier, 2019.","chicago":"Maes, Margaret E, Gloria Colombo, Rouven Schulz, and Sandra Siegert. “Targeting Microglia with Lentivirus and AAV: Recent Advances and Remaining Challenges.” <i>Neuroscience Letters</i>. Elsevier, 2019. <a href=\"https://doi.org/10.1016/j.neulet.2019.134310\">https://doi.org/10.1016/j.neulet.2019.134310</a>.","ama":"Maes ME, Colombo G, Schulz R, Siegert S. Targeting microglia with lentivirus and AAV: Recent advances and remaining challenges. <i>Neuroscience Letters</i>. 2019;707. doi:<a href=\"https://doi.org/10.1016/j.neulet.2019.134310\">10.1016/j.neulet.2019.134310</a>","mla":"Maes, Margaret E., et al. “Targeting Microglia with Lentivirus and AAV: Recent Advances and Remaining Challenges.” <i>Neuroscience Letters</i>, vol. 707, 134310, Elsevier, 2019, doi:<a href=\"https://doi.org/10.1016/j.neulet.2019.134310\">10.1016/j.neulet.2019.134310</a>.","ista":"Maes ME, Colombo G, Schulz R, Siegert S. 2019. Targeting microglia with lentivirus and AAV: Recent advances and remaining challenges. Neuroscience Letters. 707, 134310."},"ddc":["570"],"article_type":"original","abstract":[{"text":"Microglia have emerged as a critical component of neurodegenerative diseases. Genetic manipulation of microglia can elucidate their functional impact in disease. In neuroscience, recombinant viruses such as lentiviruses and adeno-associated viruses (AAVs) have been successfully used to target various cell types in the brain, although effective transduction of microglia is rare. In this review, we provide a short background of lentiviruses and AAVs, and strategies for designing recombinant viral vectors. Then, we will summarize recent literature on successful microglial transductions in vitro and in vivo, and discuss the current challenges. Finally, we provide guidelines for reporting the efficiency and specificity of viral targeting in microglia, which will enable the microglial research community to assess and improve methodologies for future studies.","lang":"eng"}],"publication_status":"published","language":[{"iso":"eng"}],"status":"public","has_accepted_license":"1","pmid":1,"quality_controlled":"1"},{"month":"10","date_updated":"2023-09-11T14:13:32Z","title":"In Vivo regulation of Oligodendrocyte processor cell proliferation and differentiation by the AMPA-receptor Subunit GluA2","article_processing_charge":"No","date_published":"2018-10-23T00:00:00Z","page":"852 - 861.e7","publication":"Cell Reports","file_date_updated":"2020-07-14T12:46:03Z","external_id":{"isi":["000448219500005"]},"date_created":"2018-12-11T11:44:16Z","_id":"32","volume":25,"oa":1,"intvolume":"        25","acknowledgement":"This work was supported by Deutsche Forschungsgemeinschaft (DFG) grant KU2569/1-1 (to M.K.); DFG project EXC307Centre for Integrative Neuroscience (CIN), including grant Pool Project 2011-12 (jointly to M.K. and I.E.); and the Charitable Hertie Foundation (to I.E.). CIN is an Excellence Cluster funded by the DFG within the framework of the Excellence Initiative for 2008–2018. M.K. is supported by the Tistou & Charlotte Kerstan Foundation.","issue":"4","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","scopus_import":"1","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png"},"department":[{"_id":"SaSi"}],"year":"2018","author":[{"full_name":"Chen, Ting","last_name":"Chen","first_name":"Ting"},{"first_name":"Bartosz","full_name":"Kula, Bartosz","last_name":"Kula"},{"orcid":"0000-0002-4002-4686","full_name":"Nagy, Balint","last_name":"Nagy","id":"30F830CE-02D1-11E9-9BAA-DAF4881429F2","first_name":"Balint"},{"full_name":"Barzan, Ruxandra","last_name":"Barzan","first_name":"Ruxandra"},{"last_name":"Gall","full_name":"Gall, Andrea","first_name":"Andrea"},{"last_name":"Ehrlich","full_name":"Ehrlich, Ingrid","first_name":"Ingrid"},{"full_name":"Kukley, Maria","last_name":"Kukley","first_name":"Maria"}],"isi":1,"oa_version":"Published Version","doi":"10.1016/j.celrep.2018.09.066","day":"23","publisher":"Elsevier","status":"public","has_accepted_license":"1","language":[{"iso":"eng"}],"publist_id":"8023","publication_status":"published","abstract":[{"text":"The functional role of AMPA receptor (AMPAR)-mediated synaptic signaling between neurons and oligodendrocyte precursor cells (OPCs) remains enigmatic. We modified the properties of AMPARs at axon-OPC synapses in the mouse corpus callosum in vivo during the peak of myelination by targeting the GluA2 subunit. Expression of the unedited (Ca2+ permeable) or the pore-dead GluA2 subunit of AMPARs triggered proliferation of OPCs and reduced their differentiation into oligodendrocytes. Expression of the cytoplasmic C-terminal (GluA2(813-862)) of the GluA2 subunit (C-tail), a modification designed to affect the interaction between GluA2 and AMPAR-binding proteins and to perturb trafficking of GluA2-containing AMPARs, decreased the differentiation of OPCs without affecting their proliferation. These findings suggest that ionotropic and non-ionotropic properties of AMPARs in OPCs, as well as specific aspects of AMPAR-mediated signaling at axon-OPC synapses in the mouse corpus callosum, are important for balancing the response of OPCs to proliferation and differentiation cues. In the brain, oligodendrocyte precursor cells (OPCs) receive glutamatergic AMPA-receptor-mediated synaptic input from neurons. Chen et al. show that modifying AMPA-receptor properties at axon-OPC synapses alters proliferation and differentiation of OPCs. This expands the traditional view of synaptic transmission by suggesting neurons also use synapses to modulate behavior of glia.","lang":"eng"}],"quality_controlled":"1","type":"journal_article","citation":{"ama":"Chen T, Kula B, Nagy B, et al. In Vivo regulation of Oligodendrocyte processor cell proliferation and differentiation by the AMPA-receptor Subunit GluA2. <i>Cell Reports</i>. 2018;25(4):852-861.e7. doi:<a href=\"https://doi.org/10.1016/j.celrep.2018.09.066\">10.1016/j.celrep.2018.09.066</a>","chicago":"Chen, Ting, Bartosz Kula, Balint Nagy, Ruxandra Barzan, Andrea Gall, Ingrid Ehrlich, and Maria Kukley. “In Vivo Regulation of Oligodendrocyte Processor Cell Proliferation and Differentiation by the AMPA-Receptor Subunit GluA2.” <i>Cell Reports</i>. Elsevier, 2018. <a href=\"https://doi.org/10.1016/j.celrep.2018.09.066\">https://doi.org/10.1016/j.celrep.2018.09.066</a>.","ista":"Chen T, Kula B, Nagy B, Barzan R, Gall A, Ehrlich I, Kukley M. 2018. In Vivo regulation of Oligodendrocyte processor cell proliferation and differentiation by the AMPA-receptor Subunit GluA2. Cell Reports. 25(4), 852–861.e7.","mla":"Chen, Ting, et al. “In Vivo Regulation of Oligodendrocyte Processor Cell Proliferation and Differentiation by the AMPA-Receptor Subunit GluA2.” <i>Cell Reports</i>, vol. 25, no. 4, Elsevier, 2018, p. 852–861.e7, doi:<a href=\"https://doi.org/10.1016/j.celrep.2018.09.066\">10.1016/j.celrep.2018.09.066</a>.","short":"T. Chen, B. Kula, B. Nagy, R. Barzan, A. Gall, I. Ehrlich, M. Kukley, Cell Reports 25 (2018) 852–861.e7.","apa":"Chen, T., Kula, B., Nagy, B., Barzan, R., Gall, A., Ehrlich, I., &#38; Kukley, M. (2018). In Vivo regulation of Oligodendrocyte processor cell proliferation and differentiation by the AMPA-receptor Subunit GluA2. <i>Cell Reports</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.celrep.2018.09.066\">https://doi.org/10.1016/j.celrep.2018.09.066</a>","ieee":"T. Chen <i>et al.</i>, “In Vivo regulation of Oligodendrocyte processor cell proliferation and differentiation by the AMPA-receptor Subunit GluA2,” <i>Cell Reports</i>, vol. 25, no. 4. Elsevier, p. 852–861.e7, 2018."},"file":[{"file_id":"5703","date_created":"2018-12-17T12:42:57Z","creator":"dernst","file_size":4461997,"date_updated":"2020-07-14T12:46:03Z","content_type":"application/pdf","checksum":"d9f74277fd57176e04732707d575cf08","relation":"main_file","access_level":"open_access","file_name":"2018_CellReports_Chen.pdf"}],"ddc":["570"]},{"ddc":["576","610"],"file":[{"access_level":"open_access","file_name":"IST-2017-889-v1+1_journal.pbio.2001993.pdf","content_type":"application/pdf","date_updated":"2020-07-14T12:47:49Z","file_size":18155365,"relation":"main_file","checksum":"0c974f430682dc832ea7b27ab5a93124","date_created":"2018-12-12T10:15:35Z","creator":"system","file_id":"5156"}],"type":"journal_article","citation":{"apa":"Nagy, B., Hovhannisyan, A., Barzan, R., Chen, T., &#38; Kukley, M. (2017). Different patterns of neuronal activity trigger distinct responses of oligodendrocyte precursor cells in the corpus callosum. <i>PLoS Biology</i>. Public Library of Science. <a href=\"https://doi.org/10.1371/journal.pbio.2001993\">https://doi.org/10.1371/journal.pbio.2001993</a>","short":"B. Nagy, A. Hovhannisyan, R. Barzan, T. Chen, M. Kukley, PLoS Biology 15 (2017).","ieee":"B. Nagy, A. Hovhannisyan, R. Barzan, T. Chen, and M. Kukley, “Different patterns of neuronal activity trigger distinct responses of oligodendrocyte precursor cells in the corpus callosum,” <i>PLoS Biology</i>, vol. 15, no. 8. Public Library of Science, 2017.","chicago":"Nagy, Balint, Anahit Hovhannisyan, Ruxandra Barzan, Ting Chen, and Maria Kukley. “Different Patterns of Neuronal Activity Trigger Distinct Responses of Oligodendrocyte Precursor Cells in the Corpus Callosum.” <i>PLoS Biology</i>. Public Library of Science, 2017. <a href=\"https://doi.org/10.1371/journal.pbio.2001993\">https://doi.org/10.1371/journal.pbio.2001993</a>.","ama":"Nagy B, Hovhannisyan A, Barzan R, Chen T, Kukley M. Different patterns of neuronal activity trigger distinct responses of oligodendrocyte precursor cells in the corpus callosum. <i>PLoS Biology</i>. 2017;15(8). doi:<a href=\"https://doi.org/10.1371/journal.pbio.2001993\">10.1371/journal.pbio.2001993</a>","mla":"Nagy, Balint, et al. “Different Patterns of Neuronal Activity Trigger Distinct Responses of Oligodendrocyte Precursor Cells in the Corpus Callosum.” <i>PLoS Biology</i>, vol. 15, no. 8, e2001993, Public Library of Science, 2017, doi:<a href=\"https://doi.org/10.1371/journal.pbio.2001993\">10.1371/journal.pbio.2001993</a>.","ista":"Nagy B, Hovhannisyan A, Barzan R, Chen T, Kukley M. 2017. Different patterns of neuronal activity trigger distinct responses of oligodendrocyte precursor cells in the corpus callosum. PLoS Biology. 15(8), e2001993."},"quality_controlled":"1","publist_id":"6983","publication_status":"published","abstract":[{"text":"In the developing and adult brain, oligodendrocyte precursor cells (OPCs) are influenced by neuronal activity: they are involved in synaptic signaling with neurons, and their proliferation and differentiation into myelinating glia can be altered by transient changes in neuronal firing. An important question that has been unanswered is whether OPCs can discriminate different patterns of neuronal activity and respond to them in a distinct way. Here, we demonstrate in brain slices that the pattern of neuronal activity determines the functional changes triggered at synapses between axons and OPCs. Furthermore, we show that stimulation of the corpus callosum at different frequencies in vivo affects proliferation and differentiation of OPCs in a dissimilar way. Our findings suggest that neurons do not influence OPCs in “all-or-none” fashion but use their firing pattern to tune the response and behavior of these nonneuronal cells.","lang":"eng"}],"has_accepted_license":"1","status":"public","language":[{"iso":"eng"}],"publisher":"Public Library of Science","day":"22","doi":"10.1371/journal.pbio.2001993","oa_version":"Published Version","year":"2017","author":[{"orcid":"0000-0002-4002-4686","full_name":"Nagy, Balint","last_name":"Nagy","id":"30F830CE-02D1-11E9-9BAA-DAF4881429F2","first_name":"Balint"},{"last_name":"Hovhannisyan","full_name":"Hovhannisyan, Anahit","first_name":"Anahit"},{"first_name":"Ruxandra","full_name":"Barzan, Ruxandra","last_name":"Barzan"},{"last_name":"Chen","full_name":"Chen, Ting","first_name":"Ting"},{"first_name":"Maria","last_name":"Kukley","full_name":"Kukley, Maria"}],"publication_identifier":{"issn":["15449173"]},"department":[{"_id":"SaSi"}],"tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"scopus_import":1,"article_number":"e2001993","oa":1,"intvolume":"        15","issue":"8","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","volume":15,"_id":"708","date_created":"2018-12-11T11:48:03Z","publication":"PLoS Biology","file_date_updated":"2020-07-14T12:47:49Z","pubrep_id":"889","date_published":"2017-08-22T00:00:00Z","title":"Different patterns of neuronal activity trigger distinct responses of oligodendrocyte precursor cells in the corpus callosum","date_updated":"2021-01-12T08:11:45Z","month":"08"},{"quality_controlled":"1","publication_status":"published","abstract":[{"text":"PURPOSE. Gene therapy of retinal ganglion cells (RGCs) has promise as a powerful therapeutic for the rescue and regeneration of these cells after optic nerve damage. However, early after damage, RGCs undergo atrophic changes, including gene silencing. It is not known if these changes will deleteriously affect transduction and transgene expression, or if the therapeutic protein can influence reactivation of the endogenous genome. METHODS. Double-transgenic mice carrying a Rosa26-(LoxP)-tdTomato reporter, and a mutant allele for the proapoptotic Bax gene were reared. The Bax mutant blocks apoptosis, but RGCs still exhibit nuclear atrophy and gene silencing. At times ranging from 1 hour to 4 weeks after optic nerve crush (ONC), eyes received an intravitreal injection of AAV2 virus carrying the Cre recombinase. Successful transduction was monitored by expression of the tdTomato reporter. Immunostaining was used to localize tdTomato expression in select cell types. RESULTS. Successful transduction of RGCs was achieved at all time points after ONC using AAV2 expressing Cre from the phosphoglycerate kinase (Pgk) promoter, but not the CMV promoter. ONC promoted an increase in the transduction of cell types in the inner nuclear layer, including Müller cells and rod bipolar neurons. There was minimal evidence of transduction of amacrine cells and astrocytes in the inner retina or optic nerve. CONCLUSIONS. Damaged RGCs can be transduced and at least some endogenous genes can be subsequently activated. Optic nerve damage may change retinal architecture to allow greater penetration of an AAV2 virus to transduce several additional cell types in the inner nuclear layer.","lang":"eng"}],"publist_id":"7254","language":[{"iso":"eng"}],"has_accepted_license":"1","status":"public","ddc":["576"],"file":[{"date_created":"2018-12-12T10:17:53Z","creator":"system","file_id":"5311","access_level":"open_access","file_name":"IST-2018-920-v1+1_i1552-5783-58-14-6091.pdf","file_size":2955559,"date_updated":"2020-07-14T12:47:04Z","content_type":"application/pdf","checksum":"d7a7b6f1fa9211a04e5e65634a0265d9","relation":"main_file"}],"type":"journal_article","citation":{"mla":"Nickells, Robert, et al. “AAV2 Mediated Transduction of the Mouse Retina after Optic Nerve Injury.” <i>Investigative Ophthalmology and Visual Science</i>, vol. 58, no. 14, Association for Research in Vision and Ophthalmology, 2017, pp. 6091–104, doi:<a href=\"https://doi.org/10.1167/iovs.17-22634\">10.1167/iovs.17-22634</a>.","ista":"Nickells R, Schmitt H, Maes ME, Schlamp C. 2017. AAV2 mediated transduction of the mouse retina after optic nerve injury. Investigative Ophthalmology and Visual Science. 58(14), 6091–6104.","chicago":"Nickells, Robert, Heather Schmitt, Margaret E Maes, and Cassandra Schlamp. “AAV2 Mediated Transduction of the Mouse Retina after Optic Nerve Injury.” <i>Investigative Ophthalmology and Visual Science</i>. Association for Research in Vision and Ophthalmology, 2017. <a href=\"https://doi.org/10.1167/iovs.17-22634\">https://doi.org/10.1167/iovs.17-22634</a>.","ama":"Nickells R, Schmitt H, Maes ME, Schlamp C. AAV2 mediated transduction of the mouse retina after optic nerve injury. <i>Investigative Ophthalmology and Visual Science</i>. 2017;58(14):6091-6104. doi:<a href=\"https://doi.org/10.1167/iovs.17-22634\">10.1167/iovs.17-22634</a>","ieee":"R. Nickells, H. Schmitt, M. E. Maes, and C. Schlamp, “AAV2 mediated transduction of the mouse retina after optic nerve injury,” <i>Investigative Ophthalmology and Visual Science</i>, vol. 58, no. 14. Association for Research in Vision and Ophthalmology, pp. 6091–6104, 2017.","apa":"Nickells, R., Schmitt, H., Maes, M. E., &#38; Schlamp, C. (2017). AAV2 mediated transduction of the mouse retina after optic nerve injury. <i>Investigative Ophthalmology and Visual Science</i>. Association for Research in Vision and Ophthalmology. <a href=\"https://doi.org/10.1167/iovs.17-22634\">https://doi.org/10.1167/iovs.17-22634</a>","short":"R. Nickells, H. Schmitt, M.E. Maes, C. 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How MicroRNAs Are involved in splitting the mind. JAMA Psychiatry. 73(4), 409–410.","mla":"Tsai, Lihuei, and Sandra Siegert. “How MicroRNAs Are Involved in Splitting the Mind.” <i>JAMA Psychiatry</i>, vol. 73, no. 4, American Medical Association, 2016, pp. 409–10, doi:<a href=\"https://doi.org/10.1001/jamapsychiatry.2015.3144\">10.1001/jamapsychiatry.2015.3144</a>.","chicago":"Tsai, Lihuei, and Sandra Siegert. “How MicroRNAs Are Involved in Splitting the Mind.” <i>JAMA Psychiatry</i>. American Medical Association, 2016. <a href=\"https://doi.org/10.1001/jamapsychiatry.2015.3144\">https://doi.org/10.1001/jamapsychiatry.2015.3144</a>.","ama":"Tsai L, Siegert S. How MicroRNAs Are involved in splitting the mind. <i>JAMA Psychiatry</i>. 2016;73(4):409-410. doi:<a href=\"https://doi.org/10.1001/jamapsychiatry.2015.3144\">10.1001/jamapsychiatry.2015.3144</a>","ieee":"L. Tsai and S. Siegert, “How MicroRNAs Are involved in splitting the mind,” <i>JAMA Psychiatry</i>, vol. 73, no. 4. 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