[{"department":[{"_id":"NanoFab"}],"article_processing_charge":"No","date_created":"2020-05-03T22:00:49Z","publication_status":"published","intvolume":"        19","title":"Broad spectral tuning of ultra-low-loss polaritons in a van der Waals crystal by intercalation","scopus_import":"1","_id":"7792","pmid":1,"author":[{"full_name":"Taboada-Gutiérrez, Javier","last_name":"Taboada-Gutiérrez","first_name":"Javier"},{"last_name":"Álvarez-Pérez","first_name":"Gonzalo","full_name":"Álvarez-Pérez, Gonzalo"},{"first_name":"Jiahua","last_name":"Duan","full_name":"Duan, Jiahua"},{"full_name":"Ma, Weiliang","last_name":"Ma","first_name":"Weiliang"},{"last_name":"Crowley","first_name":"Kyle","full_name":"Crowley, Kyle"},{"id":"2A307FE2-F248-11E8-B48F-1D18A9856A87","full_name":"Prieto Gonzalez, Ivan","orcid":"0000-0002-7370-5357","last_name":"Prieto Gonzalez","first_name":"Ivan"},{"full_name":"Bylinkin, Andrei","first_name":"Andrei","last_name":"Bylinkin"},{"last_name":"Autore","first_name":"Marta","full_name":"Autore, Marta"},{"last_name":"Volkova","first_name":"Halyna","full_name":"Volkova, Halyna"},{"first_name":"Kenta","last_name":"Kimura","full_name":"Kimura, Kenta"},{"first_name":"Tsuyoshi","last_name":"Kimura","full_name":"Kimura, Tsuyoshi"},{"full_name":"Berger, M. H.","last_name":"Berger","first_name":"M. H."},{"last_name":"Li","first_name":"Shaojuan","full_name":"Li, Shaojuan"},{"full_name":"Bao, Qiaoliang","last_name":"Bao","first_name":"Qiaoliang"},{"last_name":"Gao","first_name":"Xuan P.A.","full_name":"Gao, Xuan P.A."},{"full_name":"Errea, Ion","last_name":"Errea","first_name":"Ion"},{"full_name":"Nikitin, Alexey Y.","first_name":"Alexey Y.","last_name":"Nikitin"},{"first_name":"Rainer","last_name":"Hillenbrand","full_name":"Hillenbrand, Rainer"},{"last_name":"Martín-Sánchez","first_name":"Javier","full_name":"Martín-Sánchez, Javier"},{"first_name":"Pablo","last_name":"Alonso-González","full_name":"Alonso-González, Pablo"}],"publisher":"Springer Nature","article_type":"original","quality_controlled":"1","page":"964–968","day":"01","doi":"10.1038/s41563-020-0665-0","abstract":[{"text":"Phonon polaritons—light coupled to lattice vibrations—in polar van der Waals crystals are promising candidates for controlling the flow of energy on the nanoscale due to their strong field confinement, anisotropic propagation and ultra-long lifetime in the picosecond range1,2,3,4,5. However, the lack of tunability of their narrow and material-specific spectral range—the Reststrahlen band—severely limits their technological implementation. Here, we demonstrate that intercalation of Na atoms in the van der Waals semiconductor α-V2O5 enables a broad spectral shift of Reststrahlen bands, and that the phonon polaritons excited show ultra-low losses (lifetime of 4 ± 1 ps), similar to phonon polaritons in a non-intercalated crystal (lifetime of 6 ± 1 ps). We expect our intercalation method to be applicable to other van der Waals crystals, opening the door for the use of phonon polaritons in broad spectral bands in the mid-infrared domain.","lang":"eng"}],"year":"2020","citation":{"ista":"Taboada-Gutiérrez J, Álvarez-Pérez G, Duan J, Ma W, Crowley K, Prieto Gonzalez I, Bylinkin A, Autore M, Volkova H, Kimura K, Kimura T, Berger MH, Li S, Bao Q, Gao XPA, Errea I, Nikitin AY, Hillenbrand R, Martín-Sánchez J, Alonso-González P. 2020. Broad spectral tuning of ultra-low-loss polaritons in a van der Waals crystal by intercalation. Nature Materials. 19, 964–968.","mla":"Taboada-Gutiérrez, Javier, et al. “Broad Spectral Tuning of Ultra-Low-Loss Polaritons in a van Der Waals Crystal by Intercalation.” <i>Nature Materials</i>, vol. 19, Springer Nature, 2020, pp. 964–968, doi:<a href=\"https://doi.org/10.1038/s41563-020-0665-0\">10.1038/s41563-020-0665-0</a>.","short":"J. Taboada-Gutiérrez, G. Álvarez-Pérez, J. Duan, W. Ma, K. Crowley, I. Prieto Gonzalez, A. Bylinkin, M. Autore, H. Volkova, K. Kimura, T. Kimura, M.H. Berger, S. Li, Q. Bao, X.P.A. Gao, I. Errea, A.Y. Nikitin, R. Hillenbrand, J. Martín-Sánchez, P. Alonso-González, Nature Materials 19 (2020) 964–968.","ieee":"J. Taboada-Gutiérrez <i>et al.</i>, “Broad spectral tuning of ultra-low-loss polaritons in a van der Waals crystal by intercalation,” <i>Nature Materials</i>, vol. 19. Springer Nature, pp. 964–968, 2020.","chicago":"Taboada-Gutiérrez, Javier, Gonzalo Álvarez-Pérez, Jiahua Duan, Weiliang Ma, Kyle Crowley, Ivan Prieto Gonzalez, Andrei Bylinkin, et al. “Broad Spectral Tuning of Ultra-Low-Loss Polaritons in a van Der Waals Crystal by Intercalation.” <i>Nature Materials</i>. Springer Nature, 2020. <a href=\"https://doi.org/10.1038/s41563-020-0665-0\">https://doi.org/10.1038/s41563-020-0665-0</a>.","apa":"Taboada-Gutiérrez, J., Álvarez-Pérez, G., Duan, J., Ma, W., Crowley, K., Prieto Gonzalez, I., … Alonso-González, P. (2020). Broad spectral tuning of ultra-low-loss polaritons in a van der Waals crystal by intercalation. <i>Nature Materials</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41563-020-0665-0\">https://doi.org/10.1038/s41563-020-0665-0</a>","ama":"Taboada-Gutiérrez J, Álvarez-Pérez G, Duan J, et al. Broad spectral tuning of ultra-low-loss polaritons in a van der Waals crystal by intercalation. <i>Nature Materials</i>. 2020;19:964–968. doi:<a href=\"https://doi.org/10.1038/s41563-020-0665-0\">10.1038/s41563-020-0665-0</a>"},"date_updated":"2023-08-21T06:18:20Z","external_id":{"pmid":["32284598"],"isi":["000526218500004"]},"isi":1,"acknowledgement":"J.T.-G. and G.Á.-P. acknowledge support through the Severo Ochoa Program from the Government of the Principality of Asturias (nos. PA-18-PF-BP17-126 and PA-20-PF-BP19-053, respectively). J.M.-S. acknowledges finantial support from the Clarín Programme from the Government of the Principality of Asturias and a Marie Curie-COFUND grant (PA-18-ACB17-29) and the Ramón y Cajal Program from the Government of Spain (RYC2018-026196-I). K.C., X.P.A.G., H.V. and M.H.B. acknowledge the Air Force Office of Scientific Research (AFOSR) grant no. FA 9550-18-1-0030 for funding support. I.E. acknowledges financial support from the Spanish Ministry of Economy and Competitiveness (grant no. FIS2016-76617-P). A.Y.N. acknowledges the Spanish Ministry of Science, Innovation and Universities (national project no. MAT2017-88358-C3-3-R) and the Basque Government (grant no. IT1164-19). Q.B. acknowledges the support from Australian Research Council (grant nos. FT150100450, IH150100006 and CE170100039). R.H. acknowledges support from the Spanish Ministry of Economy, Industry, and Competitiveness (national project RTI2018-094830-B-100 and the Project MDM-2016-0618 of the María de Maeztu Units of Excellence Program) and the Basque Goverment (grant no. IT1164-19). P.A.-G. acknowledges support from the European Research Council under starting grant no. 715496, 2DNANOPTICA.","volume":19,"oa_version":"None","month":"09","publication":"Nature Materials","language":[{"iso":"eng"}],"publication_identifier":{"issn":["14761122"],"eissn":["14764660"]},"type":"journal_article","date_published":"2020-09-01T00:00:00Z","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","status":"public"},{"article_number":"e201907154","month":"06","project":[{"_id":"25A603A2-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","name":"Cytoskeletal force generation and force transduction of migrating leukocytes","grant_number":"281556"},{"grant_number":"724373","name":"Cellular navigation along spatial gradients","_id":"25FE9508-B435-11E9-9278-68D0E5697425","call_identifier":"H2020"},{"call_identifier":"FWF","_id":"26018E70-B435-11E9-9278-68D0E5697425","grant_number":"P29911","name":"Mechanical adaptation of lamellipodial actin"},{"call_identifier":"FWF","_id":"252C3B08-B435-11E9-9278-68D0E5697425","name":"Nano-Analytics of Cellular Systems","grant_number":"W 1250-B20"},{"call_identifier":"FP7","_id":"25681D80-B435-11E9-9278-68D0E5697425","grant_number":"291734","name":"International IST Postdoc Fellowship Programme"},{"_id":"25A48D24-B435-11E9-9278-68D0E5697425","name":"Molecular and system level view of immune cell migration","grant_number":"ALTF 1396-2014"}],"oa_version":"Published Version","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"},{"_id":"PreCl"}],"has_accepted_license":"1","publication":"The Journal of Cell Biology","language":[{"iso":"eng"}],"oa":1,"publication_identifier":{"eissn":["1540-8140"]},"type":"journal_article","date_published":"2020-06-01T00:00:00Z","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"status":"public","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","file":[{"relation":"main_file","access_level":"open_access","success":1,"file_id":"8801","creator":"dernst","date_created":"2020-11-24T13:25:13Z","checksum":"cb0b9c77842ae1214caade7b77e4d82d","file_size":7536712,"date_updated":"2020-11-24T13:25:13Z","content_type":"application/pdf","file_name":"2020_JCellBiol_Kopf.pdf"}],"intvolume":"       219","title":"Microtubules control cellular shape and coherence in amoeboid migrating cells","article_processing_charge":"No","department":[{"_id":"MiSi"},{"_id":"Bio"},{"_id":"NanoFab"}],"date_created":"2020-05-24T22:00:56Z","publication_status":"published","issue":"6","author":[{"first_name":"Aglaja","last_name":"Kopf","orcid":"0000-0002-2187-6656","full_name":"Kopf, Aglaja","id":"31DAC7B6-F248-11E8-B48F-1D18A9856A87"},{"id":"3F0587C8-F248-11E8-B48F-1D18A9856A87","last_name":"Renkawitz","first_name":"Jörg","full_name":"Renkawitz, Jörg","orcid":"0000-0003-2856-3369"},{"id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild","first_name":"Robert","full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522"},{"last_name":"Girkontaite","first_name":"Irute","full_name":"Girkontaite, Irute"},{"full_name":"Tedford, Kerry","first_name":"Kerry","last_name":"Tedford"},{"orcid":"0000-0001-5145-4609","full_name":"Merrin, Jack","first_name":"Jack","last_name":"Merrin","id":"4515C308-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Thorn-Seshold, Oliver","first_name":"Oliver","last_name":"Thorn-Seshold"},{"full_name":"Trauner, Dirk","last_name":"Trauner","first_name":"Dirk","id":"E8F27F48-3EBA-11E9-92A1-B709E6697425"},{"last_name":"Häcker","first_name":"Hans","full_name":"Häcker, Hans"},{"full_name":"Fischer, Klaus Dieter","last_name":"Fischer","first_name":"Klaus Dieter"},{"id":"3EB04B78-F248-11E8-B48F-1D18A9856A87","last_name":"Kiermaier","first_name":"Eva","full_name":"Kiermaier, Eva","orcid":"0000-0001-6165-5738"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt","first_name":"Michael K","full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179"}],"scopus_import":"1","pmid":1,"_id":"7875","article_type":"original","publisher":"Rockefeller University Press","file_date_updated":"2020-11-24T13:25:13Z","ec_funded":1,"quality_controlled":"1","abstract":[{"text":"Cells navigating through complex tissues face a fundamental challenge: while multiple protrusions explore different paths, the cell needs to avoid entanglement. How a cell surveys and then corrects its own shape is poorly understood. Here, we demonstrate that spatially distinct microtubule dynamics regulate amoeboid cell migration by locally promoting the retraction of protrusions. In migrating dendritic cells, local microtubule depolymerization within protrusions remote from the microtubule organizing center triggers actomyosin contractility controlled by RhoA and its exchange factor Lfc. Depletion of Lfc leads to aberrant myosin localization, thereby causing two effects that rate-limit locomotion: (1) impaired cell edge coordination during path finding and (2) defective adhesion resolution. Compromised shape control is particularly hindering in geometrically complex microenvironments, where it leads to entanglement and ultimately fragmentation of the cell body. We thus demonstrate that microtubules can act as a proprioceptive device: they sense cell shape and control actomyosin retraction to sustain cellular coherence.","lang":"eng"}],"day":"01","doi":"10.1083/jcb.201907154","external_id":{"isi":["000538141100020"],"pmid":["32379884"]},"isi":1,"year":"2020","citation":{"ama":"Kopf A, Renkawitz J, Hauschild R, et al. Microtubules control cellular shape and coherence in amoeboid migrating cells. <i>The Journal of Cell Biology</i>. 2020;219(6). doi:<a href=\"https://doi.org/10.1083/jcb.201907154\">10.1083/jcb.201907154</a>","apa":"Kopf, A., Renkawitz, J., Hauschild, R., Girkontaite, I., Tedford, K., Merrin, J., … Sixt, M. K. (2020). Microtubules control cellular shape and coherence in amoeboid migrating cells. <i>The Journal of Cell Biology</i>. Rockefeller University Press. <a href=\"https://doi.org/10.1083/jcb.201907154\">https://doi.org/10.1083/jcb.201907154</a>","ieee":"A. Kopf <i>et al.</i>, “Microtubules control cellular shape and coherence in amoeboid migrating cells,” <i>The Journal of Cell Biology</i>, vol. 219, no. 6. Rockefeller University Press, 2020.","chicago":"Kopf, Aglaja, Jörg Renkawitz, Robert Hauschild, Irute Girkontaite, Kerry Tedford, Jack Merrin, Oliver Thorn-Seshold, et al. “Microtubules Control Cellular Shape and Coherence in Amoeboid Migrating Cells.” <i>The Journal of Cell Biology</i>. Rockefeller University Press, 2020. <a href=\"https://doi.org/10.1083/jcb.201907154\">https://doi.org/10.1083/jcb.201907154</a>.","short":"A. Kopf, J. Renkawitz, R. Hauschild, I. Girkontaite, K. Tedford, J. Merrin, O. Thorn-Seshold, D. Trauner, H. Häcker, K.D. Fischer, E. Kiermaier, M.K. Sixt, The Journal of Cell Biology 219 (2020).","mla":"Kopf, Aglaja, et al. “Microtubules Control Cellular Shape and Coherence in Amoeboid Migrating Cells.” <i>The Journal of Cell Biology</i>, vol. 219, no. 6, e201907154, Rockefeller University Press, 2020, doi:<a href=\"https://doi.org/10.1083/jcb.201907154\">10.1083/jcb.201907154</a>.","ista":"Kopf A, Renkawitz J, Hauschild R, Girkontaite I, Tedford K, Merrin J, Thorn-Seshold O, Trauner D, Häcker H, Fischer KD, Kiermaier E, Sixt MK. 2020. Microtubules control cellular shape and coherence in amoeboid migrating cells. The Journal of Cell Biology. 219(6), e201907154."},"date_updated":"2023-08-21T06:28:17Z","ddc":["570"],"volume":219,"acknowledgement":"The authors thank the Scientific Service Units (Life Sciences, Bioimaging, Preclinical) of the Institute of Science and Technology Austria for excellent support. This work was funded by the European Research Council (ERC StG 281556 and CoG 724373), two grants from the Austrian\r\nScience Fund (FWF; P29911 and DK Nanocell W1250-B20 to M. Sixt) and by the German Research Foundation (DFG SFB1032 project B09) to O. Thorn-Seshold and D. Trauner. J. Renkawitz was supported by ISTFELLOW funding from the People Program (Marie Curie Actions) of the European Union’s Seventh Framework Programme (FP7/2007-2013) under the Research Executive Agency grant agreement (291734) and a European Molecular Biology Organization long-term fellowship (ALTF 1396-2014) co-funded by the European Commission (LTFCOFUND2013, GA-2013-609409), E. Kiermaier by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy—EXC 2151—390873048, and H. Hacker by the American Lebanese Syrian Associated ¨Charities. K.-D. Fischer was supported by the Analysis, Imaging and Modelling of Neuronal and Inflammatory Processes graduate school funded by the Ministry of Economics, Science, and Digitisation of the State Saxony-Anhalt and by the European Funds for Social and Regional Development."},{"status":"public","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","related_material":{"record":[{"relation":"dissertation_contains","id":"14697","status":"public"},{"status":"public","relation":"dissertation_contains","id":"12401"}],"link":[{"relation":"press_release","description":"News on IST Homepage","url":"https://ist.ac.at/en/news/off-road-mode-enables-mobile-cells-to-move-freely/"}]},"type":"journal_article","date_published":"2020-06-25T00:00:00Z","publication_identifier":{"issn":["00280836"],"eissn":["14764687"]},"language":[{"iso":"eng"}],"publication":"Nature","project":[{"name":"Cytoskeletal force generation and force transduction of migrating leukocytes","grant_number":"281556","call_identifier":"FP7","_id":"25A603A2-B435-11E9-9278-68D0E5697425"},{"call_identifier":"H2020","_id":"25FE9508-B435-11E9-9278-68D0E5697425","name":"Cellular navigation along spatial gradients","grant_number":"724373"},{"name":"Mechanical adaptation of lamellipodial actin","grant_number":"P29911","call_identifier":"FWF","_id":"26018E70-B435-11E9-9278-68D0E5697425"},{"_id":"260AA4E2-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","grant_number":"747687","name":"Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells"}],"oa_version":"None","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"M-Shop"}],"month":"06","volume":582,"acknowledgement":"We thank A. Leithner and J. Renkawitz for discussion and critical reading of the manuscript; J. Schwarz and M. Mehling for establishing the microfluidic setups; the Bioimaging Facility of IST Austria for excellent support, as well as the Life Science Facility and the Miba Machine Shop of IST Austria; and F. N. Arslan, L. E. Burnett and L. Li for their work during their rotation in the IST PhD programme. This work was supported by the European Research Council (ERC StG 281556 and CoG 724373) to M.S. and grants from the Austrian Science Fund (FWF P29911) and the WWTF to M.S. M.H. was supported by the European Regional Development Fund Project (CZ.02.1.01/0.0/0.0/15_003/0000476). F.G. received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. 747687.","year":"2020","citation":{"apa":"Reversat, A., Gärtner, F. R., Merrin, J., Stopp, J. A., Tasciyan, S., Aguilera Servin, J. L., … Sixt, M. K. (2020). Cellular locomotion using environmental topography. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-020-2283-z\">https://doi.org/10.1038/s41586-020-2283-z</a>","ama":"Reversat A, Gärtner FR, Merrin J, et al. Cellular locomotion using environmental topography. <i>Nature</i>. 2020;582:582–585. doi:<a href=\"https://doi.org/10.1038/s41586-020-2283-z\">10.1038/s41586-020-2283-z</a>","ieee":"A. Reversat <i>et al.</i>, “Cellular locomotion using environmental topography,” <i>Nature</i>, vol. 582. Springer Nature, pp. 582–585, 2020.","chicago":"Reversat, Anne, Florian R Gärtner, Jack Merrin, Julian A Stopp, Saren Tasciyan, Juan L Aguilera Servin, Ingrid de Vries, et al. “Cellular Locomotion Using Environmental Topography.” <i>Nature</i>. Springer Nature, 2020. <a href=\"https://doi.org/10.1038/s41586-020-2283-z\">https://doi.org/10.1038/s41586-020-2283-z</a>.","short":"A. Reversat, F.R. Gärtner, J. Merrin, J.A. Stopp, S. Tasciyan, J.L. Aguilera Servin, I. de Vries, R. Hauschild, M. Hons, M. Piel, A. Callan-Jones, R. Voituriez, M.K. Sixt, Nature 582 (2020) 582–585.","mla":"Reversat, Anne, et al. “Cellular Locomotion Using Environmental Topography.” <i>Nature</i>, vol. 582, Springer Nature, 2020, pp. 582–585, doi:<a href=\"https://doi.org/10.1038/s41586-020-2283-z\">10.1038/s41586-020-2283-z</a>.","ista":"Reversat A, Gärtner FR, Merrin J, Stopp JA, Tasciyan S, Aguilera Servin JL, de Vries I, Hauschild R, Hons M, Piel M, Callan-Jones A, Voituriez R, Sixt MK. 2020. Cellular locomotion using environmental topography. Nature. 582, 582–585."},"date_updated":"2024-03-25T23:30:12Z","external_id":{"isi":["000532688300008"]},"isi":1,"day":"25","doi":"10.1038/s41586-020-2283-z","abstract":[{"text":"Eukaryotic cells migrate by coupling the intracellular force of the actin cytoskeleton to the environment. While force coupling is usually mediated by transmembrane adhesion receptors, especially those of the integrin family, amoeboid cells such as leukocytes can migrate extremely fast despite very low adhesive forces1. Here we show that leukocytes cannot only migrate under low adhesion but can also transmit forces in the complete absence of transmembrane force coupling. When confined within three-dimensional environments, they use the topographical features of the substrate to propel themselves. Here the retrograde flow of the actin cytoskeleton follows the texture of the substrate, creating retrograde shear forces that are sufficient to drive the cell body forwards. Notably, adhesion-dependent and adhesion-independent migration are not mutually exclusive, but rather are variants of the same principle of coupling retrograde actin flow to the environment and thus can potentially operate interchangeably and simultaneously. As adhesion-free migration is independent of the chemical composition of the environment, it renders cells completely autonomous in their locomotive behaviour.","lang":"eng"}],"ec_funded":1,"quality_controlled":"1","page":"582–585","publisher":"Springer Nature","article_type":"original","scopus_import":"1","_id":"7885","author":[{"id":"35B76592-F248-11E8-B48F-1D18A9856A87","last_name":"Reversat","first_name":"Anne","full_name":"Reversat, Anne","orcid":"0000-0003-0666-8928"},{"id":"397A88EE-F248-11E8-B48F-1D18A9856A87","last_name":"Gärtner","first_name":"Florian R","full_name":"Gärtner, Florian R","orcid":"0000-0001-6120-3723"},{"first_name":"Jack","last_name":"Merrin","orcid":"0000-0001-5145-4609","full_name":"Merrin, Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Stopp, Julian A","last_name":"Stopp","first_name":"Julian A","id":"489E3F00-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Tasciyan","first_name":"Saren","full_name":"Tasciyan, Saren","orcid":"0000-0003-1671-393X","id":"4323B49C-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Aguilera Servin, Juan L","orcid":"0000-0002-2862-8372","last_name":"Aguilera Servin","first_name":"Juan L","id":"2A67C376-F248-11E8-B48F-1D18A9856A87"},{"id":"4C7D837E-F248-11E8-B48F-1D18A9856A87","full_name":"De Vries, Ingrid","last_name":"De Vries","first_name":"Ingrid"},{"first_name":"Robert","last_name":"Hauschild","orcid":"0000-0001-9843-3522","full_name":"Hauschild, Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87"},{"id":"4167FE56-F248-11E8-B48F-1D18A9856A87","full_name":"Hons, Miroslav","orcid":"0000-0002-6625-3348","last_name":"Hons","first_name":"Miroslav"},{"full_name":"Piel, Matthieu","last_name":"Piel","first_name":"Matthieu"},{"first_name":"Andrew","last_name":"Callan-Jones","full_name":"Callan-Jones, Andrew"},{"first_name":"Raphael","last_name":"Voituriez","full_name":"Voituriez, Raphael"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt","first_name":"Michael K","full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179"}],"article_processing_charge":"No","date_created":"2020-05-24T22:01:01Z","department":[{"_id":"NanoFab"},{"_id":"Bio"},{"_id":"MiSi"}],"publication_status":"published","intvolume":"       582","title":"Cellular locomotion using environmental topography"},{"article_type":"original","publisher":"IOP Publishing","file_date_updated":"2020-10-05T13:53:59Z","quality_controlled":"1","title":"Differences in power law growth over time and indicators of COVID-19 pandemic progression worldwide","intvolume":"        17","publication_status":"published","article_processing_charge":"Yes (via OA deal)","department":[{"_id":"NanoFab"}],"date_created":"2020-10-04T22:01:35Z","author":[{"full_name":"Merrin, Jack","orcid":"0000-0001-5145-4609","last_name":"Merrin","first_name":"Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87"}],"issue":"6","_id":"8597","scopus_import":"1","ddc":["510","570"],"volume":17,"acknowledgement":"I would especially like to thank Michael Sixt for encouraging me to think about these problems while working at home due to restrictions in place. I want to thank Nick Barton, Katka Bodova, Matthew Robinson, Simon Rella, Federico Sau, Ivan Prieto, and Pradeep Kumar for useful discussions.","abstract":[{"text":"Error analysis and data visualization of positive COVID-19 cases in 27 countries have been performed up to August 8, 2020. This survey generally observes a progression from early exponential growth transitioning to an intermediate power-law growth phase, as recently suggested by Ziff and Ziff. The occurrence of logistic growth after the power-law phase with lockdowns or social distancing may be described as an effect of avoidance. A visualization of the power-law growth exponent over short time windows is qualitatively similar to the Bhatia visualization for pandemic progression. Visualizations like these can indicate the onset of second waves and may influence social policy.","lang":"eng"}],"doi":"10.1088/1478-3975/abb2db","day":"23","isi":1,"external_id":{"isi":["000575539700001"]},"date_updated":"2023-08-22T09:53:29Z","year":"2020","citation":{"ista":"Merrin J. 2020. Differences in power law growth over time and indicators of COVID-19 pandemic progression worldwide. Physical Biology. 17(6), 065005.","short":"J. Merrin, Physical Biology 17 (2020).","mla":"Merrin, Jack. “Differences in Power Law Growth over Time and Indicators of COVID-19 Pandemic Progression Worldwide.” <i>Physical Biology</i>, vol. 17, no. 6, 065005, IOP Publishing, 2020, doi:<a href=\"https://doi.org/10.1088/1478-3975/abb2db\">10.1088/1478-3975/abb2db</a>.","ieee":"J. Merrin, “Differences in power law growth over time and indicators of COVID-19 pandemic progression worldwide,” <i>Physical Biology</i>, vol. 17, no. 6. IOP Publishing, 2020.","chicago":"Merrin, Jack. “Differences in Power Law Growth over Time and Indicators of COVID-19 Pandemic Progression Worldwide.” <i>Physical Biology</i>. IOP Publishing, 2020. <a href=\"https://doi.org/10.1088/1478-3975/abb2db\">https://doi.org/10.1088/1478-3975/abb2db</a>.","ama":"Merrin J. Differences in power law growth over time and indicators of COVID-19 pandemic progression worldwide. <i>Physical Biology</i>. 2020;17(6). doi:<a href=\"https://doi.org/10.1088/1478-3975/abb2db\">10.1088/1478-3975/abb2db</a>","apa":"Merrin, J. (2020). Differences in power law growth over time and indicators of COVID-19 pandemic progression worldwide. <i>Physical Biology</i>. IOP Publishing. <a href=\"https://doi.org/10.1088/1478-3975/abb2db\">https://doi.org/10.1088/1478-3975/abb2db</a>"},"language":[{"iso":"eng"}],"month":"09","article_number":"065005","oa_version":"Published Version","publication":"Physical Biology","has_accepted_license":"1","status":"public","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","file":[{"file_id":"8609","creator":"dernst","success":1,"relation":"main_file","access_level":"open_access","date_updated":"2020-10-05T13:53:59Z","content_type":"application/pdf","file_name":"2020_PhysBio_Merrin.pdf","date_created":"2020-10-05T13:53:59Z","checksum":"fec9bdd355ed349f09990faab20838a7","file_size":1667111}],"oa":1,"publication_identifier":{"eissn":["14783975"]},"date_published":"2020-09-23T00:00:00Z","type":"journal_article","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"}},{"status":"public","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","file":[{"creator":"dernst","file_id":"7243","access_level":"open_access","relation":"main_file","file_name":"2019_Bioengineering_Merrin.pdf","content_type":"application/pdf","date_updated":"2020-07-14T12:47:54Z","file_size":2660780,"checksum":"80f1499e2a4caccdf3aa54b137fd99a0","date_created":"2020-01-07T14:49:59Z"}],"oa":1,"publication_identifier":{"eissn":["23065354"]},"date_published":"2019-12-03T00:00:00Z","type":"journal_article","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"language":[{"iso":"eng"}],"month":"12","article_number":"109","oa_version":"Published Version","publication":"Bioengineering","has_accepted_license":"1","ddc":["620"],"volume":6,"abstract":[{"lang":"eng","text":"This is a literature teaching resource review for biologically inspired microfluidics courses\r\nor exploring the diverse applications of microfluidics. The structure is around key papers and model\r\norganisms. While courses gradually change over time, a focus remains on understanding how\r\nmicrofluidics has developed as well as what it can and cannot do for researchers. As a primary\r\nstarting point, we cover micro-fluid mechanics principles and microfabrication of devices. A variety\r\nof applications are discussed using model prokaryotic and eukaryotic organisms from the set\r\nof bacteria (Escherichia coli), trypanosomes (Trypanosoma brucei), yeast (Saccharomyces cerevisiae),\r\nslime molds (Physarum polycephalum), worms (Caenorhabditis elegans), flies (Drosophila melangoster),\r\nplants (Arabidopsis thaliana), and mouse immune cells (Mus musculus). Other engineering and\r\nbiochemical methods discussed include biomimetics, organ on a chip, inkjet, droplet microfluidics,\r\nbiotic games, and diagnostics. While we have not yet reached the end-all lab on a chip,\r\nmicrofluidics can still be used effectively for specific applications."}],"doi":"10.3390/bioengineering6040109","day":"03","isi":1,"external_id":{"pmid":["31816954"],"isi":["000505590000024"]},"date_updated":"2023-09-06T14:52:49Z","year":"2019","citation":{"ieee":"J. Merrin, “Frontiers in microfluidics, a teaching resource review,” <i>Bioengineering</i>, vol. 6, no. 4. MDPI, 2019.","chicago":"Merrin, Jack. “Frontiers in Microfluidics, a Teaching Resource Review.” <i>Bioengineering</i>. MDPI, 2019. <a href=\"https://doi.org/10.3390/bioengineering6040109\">https://doi.org/10.3390/bioengineering6040109</a>.","apa":"Merrin, J. (2019). Frontiers in microfluidics, a teaching resource review. <i>Bioengineering</i>. MDPI. <a href=\"https://doi.org/10.3390/bioengineering6040109\">https://doi.org/10.3390/bioengineering6040109</a>","ama":"Merrin J. Frontiers in microfluidics, a teaching resource review. <i>Bioengineering</i>. 2019;6(4). doi:<a href=\"https://doi.org/10.3390/bioengineering6040109\">10.3390/bioengineering6040109</a>","ista":"Merrin J. 2019. Frontiers in microfluidics, a teaching resource review. Bioengineering. 6(4), 109.","short":"J. Merrin, Bioengineering 6 (2019).","mla":"Merrin, Jack. “Frontiers in Microfluidics, a Teaching Resource Review.” <i>Bioengineering</i>, vol. 6, no. 4, 109, MDPI, 2019, doi:<a href=\"https://doi.org/10.3390/bioengineering6040109\">10.3390/bioengineering6040109</a>."},"article_type":"review","publisher":"MDPI","file_date_updated":"2020-07-14T12:47:54Z","quality_controlled":"1","title":"Frontiers in microfluidics, a teaching resource review","intvolume":"         6","publication_status":"published","department":[{"_id":"NanoFab"}],"date_created":"2020-01-05T23:00:45Z","article_processing_charge":"Yes","author":[{"full_name":"Merrin, Jack","orcid":"0000-0001-5145-4609","last_name":"Merrin","first_name":"Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87"}],"issue":"4","_id":"7225","pmid":1,"scopus_import":"1"},{"type":"journal_article","date_published":"2019-04-25T00:00:00Z","oa":1,"status":"public","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","related_material":{"link":[{"url":"https://ist.ac.at/en/news/leukocytes-use-their-nucleus-as-a-ruler-to-choose-path-of-least-resistance/","relation":"press_release","description":"News on IST Homepage"}],"record":[{"id":"14697","relation":"dissertation_contains","status":"public"},{"status":"public","relation":"dissertation_contains","id":"6891"}]},"main_file_link":[{"open_access":"1","url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7217284/"}],"publication":"Nature","month":"04","project":[{"call_identifier":"FP7","_id":"25A603A2-B435-11E9-9278-68D0E5697425","grant_number":"281556","name":"Cytoskeletal force generation and force transduction of migrating leukocytes (EU)"},{"call_identifier":"H2020","_id":"25FE9508-B435-11E9-9278-68D0E5697425","name":"Cellular navigation along spatial gradients","grant_number":"724373"},{"grant_number":"W01250-B20","name":"Nano-Analytics of Cellular Systems","call_identifier":"FWF","_id":"265FAEBA-B435-11E9-9278-68D0E5697425"},{"grant_number":"291734","name":"International IST Postdoc Fellowship Programme","call_identifier":"FP7","_id":"25681D80-B435-11E9-9278-68D0E5697425"},{"name":"Molecular and system level view of immune cell migration","grant_number":"ALTF 1396-2014","_id":"25A48D24-B435-11E9-9278-68D0E5697425"}],"acknowledged_ssus":[{"_id":"SSU"}],"oa_version":"Submitted Version","language":[{"iso":"eng"}],"external_id":{"isi":["000465594200050"],"pmid":["30944468"]},"isi":1,"year":"2019","citation":{"chicago":"Renkawitz, Jörg, Aglaja Kopf, Julian A Stopp, Ingrid de Vries, Meghan K. Driscoll, Jack Merrin, Robert Hauschild, et al. “Nuclear Positioning Facilitates Amoeboid Migration along the Path of Least Resistance.” <i>Nature</i>. Springer Nature, 2019. <a href=\"https://doi.org/10.1038/s41586-019-1087-5\">https://doi.org/10.1038/s41586-019-1087-5</a>.","ieee":"J. Renkawitz <i>et al.</i>, “Nuclear positioning facilitates amoeboid migration along the path of least resistance,” <i>Nature</i>, vol. 568. Springer Nature, pp. 546–550, 2019.","ama":"Renkawitz J, Kopf A, Stopp JA, et al. Nuclear positioning facilitates amoeboid migration along the path of least resistance. <i>Nature</i>. 2019;568:546-550. doi:<a href=\"https://doi.org/10.1038/s41586-019-1087-5\">10.1038/s41586-019-1087-5</a>","apa":"Renkawitz, J., Kopf, A., Stopp, J. A., de Vries, I., Driscoll, M. K., Merrin, J., … Sixt, M. K. (2019). Nuclear positioning facilitates amoeboid migration along the path of least resistance. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-019-1087-5\">https://doi.org/10.1038/s41586-019-1087-5</a>","ista":"Renkawitz J, Kopf A, Stopp JA, de Vries I, Driscoll MK, Merrin J, Hauschild R, Welf ES, Danuser G, Fiolka R, Sixt MK. 2019. Nuclear positioning facilitates amoeboid migration along the path of least resistance. Nature. 568, 546–550.","mla":"Renkawitz, Jörg, et al. “Nuclear Positioning Facilitates Amoeboid Migration along the Path of Least Resistance.” <i>Nature</i>, vol. 568, Springer Nature, 2019, pp. 546–50, doi:<a href=\"https://doi.org/10.1038/s41586-019-1087-5\">10.1038/s41586-019-1087-5</a>.","short":"J. Renkawitz, A. Kopf, J.A. Stopp, I. de Vries, M.K. Driscoll, J. Merrin, R. Hauschild, E.S. Welf, G. Danuser, R. Fiolka, M.K. Sixt, Nature 568 (2019) 546–550."},"date_updated":"2024-03-25T23:30:22Z","abstract":[{"lang":"eng","text":"During metazoan development, immune surveillance and cancer dissemination, cells migrate in complex three-dimensional microenvironments1,2,3. These spaces are crowded by cells and extracellular matrix, generating mazes with differently sized gaps that are typically smaller than the diameter of the migrating cell4,5. Most mesenchymal and epithelial cells and some—but not all—cancer cells actively generate their migratory path using pericellular tissue proteolysis6. By contrast, amoeboid cells such as leukocytes use non-destructive strategies of locomotion7, raising the question how these extremely fast cells navigate through dense tissues. Here we reveal that leukocytes sample their immediate vicinity for large pore sizes, and are thereby able to choose the path of least resistance. This allows them to circumnavigate local obstacles while effectively following global directional cues such as chemotactic gradients. Pore-size discrimination is facilitated by frontward positioning of the nucleus, which enables the cells to use their bulkiest compartment as a mechanical gauge. Once the nucleus and the closely associated microtubule organizing centre pass the largest pore, cytoplasmic protrusions still lingering in smaller pores are retracted. These retractions are coordinated by dynamic microtubules; when microtubules are disrupted, migrating cells lose coherence and frequently fragment into migratory cytoplasmic pieces. As nuclear positioning in front of the microtubule organizing centre is a typical feature of amoeboid migration, our findings link the fundamental organization of cellular polarity to the strategy of locomotion."}],"day":"25","doi":"10.1038/s41586-019-1087-5","volume":568,"author":[{"id":"3F0587C8-F248-11E8-B48F-1D18A9856A87","first_name":"Jörg","last_name":"Renkawitz","orcid":"0000-0003-2856-3369","full_name":"Renkawitz, Jörg"},{"id":"31DAC7B6-F248-11E8-B48F-1D18A9856A87","last_name":"Kopf","first_name":"Aglaja","full_name":"Kopf, Aglaja","orcid":"0000-0002-2187-6656"},{"last_name":"Stopp","first_name":"Julian A","full_name":"Stopp, Julian A","id":"489E3F00-F248-11E8-B48F-1D18A9856A87"},{"id":"4C7D837E-F248-11E8-B48F-1D18A9856A87","first_name":"Ingrid","last_name":"de Vries","full_name":"de Vries, Ingrid"},{"first_name":"Meghan K.","last_name":"Driscoll","full_name":"Driscoll, Meghan K."},{"first_name":"Jack","last_name":"Merrin","orcid":"0000-0001-5145-4609","full_name":"Merrin, Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87"},{"id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9843-3522","full_name":"Hauschild, Robert","first_name":"Robert","last_name":"Hauschild"},{"full_name":"Welf, Erik S.","last_name":"Welf","first_name":"Erik S."},{"last_name":"Danuser","first_name":"Gaudenz","full_name":"Danuser, Gaudenz"},{"full_name":"Fiolka, Reto","last_name":"Fiolka","first_name":"Reto"},{"full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179","last_name":"Sixt","first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"}],"scopus_import":"1","_id":"6328","pmid":1,"intvolume":"       568","title":"Nuclear positioning facilitates amoeboid migration along the path of least resistance","department":[{"_id":"MiSi"},{"_id":"NanoFab"},{"_id":"Bio"}],"article_processing_charge":"No","date_created":"2019-04-17T06:52:28Z","publication_status":"published","quality_controlled":"1","ec_funded":1,"page":"546-550","article_type":"letter_note","publisher":"Springer Nature"},{"date_updated":"2023-09-15T12:11:03Z","year":"2018","citation":{"apa":"Fendrych, M., Akhmanova, M., Merrin, J., Glanc, M., Hagihara, S., Takahashi, K., … Friml, J. (2018). Rapid and reversible root growth inhibition by TIR1 auxin signalling. <i>Nature Plants</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41477-018-0190-1\">https://doi.org/10.1038/s41477-018-0190-1</a>","ama":"Fendrych M, Akhmanova M, Merrin J, et al. Rapid and reversible root growth inhibition by TIR1 auxin signalling. <i>Nature Plants</i>. 2018;4(7):453-459. doi:<a href=\"https://doi.org/10.1038/s41477-018-0190-1\">10.1038/s41477-018-0190-1</a>","chicago":"Fendrych, Matyas, Maria Akhmanova, Jack Merrin, Matous Glanc, Shinya Hagihara, Koji Takahashi, Naoyuki Uchida, Keiko U Torii, and Jiří Friml. “Rapid and Reversible Root Growth Inhibition by TIR1 Auxin Signalling.” <i>Nature Plants</i>. Springer Nature, 2018. <a href=\"https://doi.org/10.1038/s41477-018-0190-1\">https://doi.org/10.1038/s41477-018-0190-1</a>.","ieee":"M. Fendrych <i>et al.</i>, “Rapid and reversible root growth inhibition by TIR1 auxin signalling,” <i>Nature Plants</i>, vol. 4, no. 7. Springer Nature, pp. 453–459, 2018.","mla":"Fendrych, Matyas, et al. “Rapid and Reversible Root Growth Inhibition by TIR1 Auxin Signalling.” <i>Nature Plants</i>, vol. 4, no. 7, Springer Nature, 2018, pp. 453–59, doi:<a href=\"https://doi.org/10.1038/s41477-018-0190-1\">10.1038/s41477-018-0190-1</a>.","short":"M. Fendrych, M. Akhmanova, J. Merrin, M. Glanc, S. Hagihara, K. Takahashi, N. Uchida, K.U. Torii, J. Friml, Nature Plants 4 (2018) 453–459.","ista":"Fendrych M, Akhmanova M, Merrin J, Glanc M, Hagihara S, Takahashi K, Uchida N, Torii KU, Friml J. 2018. Rapid and reversible root growth inhibition by TIR1 auxin signalling. Nature Plants. 4(7), 453–459."},"isi":1,"external_id":{"pmid":["29942048"],"isi":["000443221200017"]},"doi":"10.1038/s41477-018-0190-1","day":"25","abstract":[{"text":"The phytohormone auxin is the information carrier in a plethora of developmental and physiological processes in plants(1). It has been firmly established that canonical, nuclear auxin signalling acts through regulation of gene transcription(2). Here, we combined microfluidics, live imaging, genetic engineering and computational modelling to reanalyse the classical case of root growth inhibition(3) by auxin. We show that Arabidopsis roots react to addition and removal of auxin by extremely rapid adaptation of growth rate. This process requires intracellular auxin perception but not transcriptional reprogramming. The formation of the canonical TIR1/AFB-Aux/IAA co-receptor complex is required for the growth regulation, hinting to a novel, non-transcriptional branch of this signalling pathway. Our results challenge the current understanding of root growth regulation by auxin and suggest another, presumably non-transcriptional, signalling output of the canonical auxin pathway.","lang":"eng"}],"volume":4,"_id":"192","pmid":1,"scopus_import":"1","author":[{"last_name":"Fendrych","first_name":"Matyas","full_name":"Fendrych, Matyas","orcid":"0000-0002-9767-8699","id":"43905548-F248-11E8-B48F-1D18A9856A87"},{"id":"3425EC26-F248-11E8-B48F-1D18A9856A87","full_name":"Akhmanova, Maria","orcid":"0000-0003-1522-3162","last_name":"Akhmanova","first_name":"Maria"},{"last_name":"Merrin","first_name":"Jack","full_name":"Merrin, Jack","orcid":"0000-0001-5145-4609","id":"4515C308-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Matous","last_name":"Glanc","full_name":"Glanc, Matous"},{"first_name":"Shinya","last_name":"Hagihara","full_name":"Hagihara, Shinya"},{"full_name":"Takahashi, Koji","first_name":"Koji","last_name":"Takahashi"},{"full_name":"Uchida, Naoyuki","first_name":"Naoyuki","last_name":"Uchida"},{"full_name":"Torii, Keiko U","first_name":"Keiko U","last_name":"Torii"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jirí","last_name":"Friml","orcid":"0000-0002-8302-7596","full_name":"Friml, Jirí"}],"issue":"7","publication_status":"published","article_processing_charge":"No","department":[{"_id":"JiFr"},{"_id":"DaSi"},{"_id":"NanoFab"}],"date_created":"2018-12-11T11:45:07Z","title":"Rapid and reversible root growth inhibition by TIR1 auxin signalling","intvolume":"         4","page":"453 - 459","quality_controlled":"1","publisher":"Springer Nature","article_type":"original","date_published":"2018-06-25T00:00:00Z","type":"journal_article","oa":1,"publist_id":"7728","main_file_link":[{"url":"https://www.ncbi.nlm.nih.gov/pubmed/29942048","open_access":"1"}],"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","related_material":{"link":[{"url":"https://ist.ac.at/en/news/new-mechanism-for-the-plant-hormone-auxin-discovered/","relation":"press_release","description":"News on IST Homepage"}]},"status":"public","publication":"Nature Plants","oa_version":"Submitted Version","month":"06","language":[{"iso":"eng"}]},{"title":"Micro-engineered “pillar forests” to study cell migration in complex but controlled 3D environments","intvolume":"       147","publication_status":"published","department":[{"_id":"MiSi"},{"_id":"NanoFab"}],"article_processing_charge":"No","date_created":"2018-12-11T11:44:54Z","author":[{"id":"3F0587C8-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2856-3369","full_name":"Renkawitz, Jörg","first_name":"Jörg","last_name":"Renkawitz"},{"orcid":"0000-0003-0666-8928","full_name":"Reversat, Anne","first_name":"Anne","last_name":"Reversat","id":"35B76592-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Alexander F","last_name":"Leithner","orcid":"0000-0002-1073-744X","full_name":"Leithner, Alexander F","id":"3B1B77E4-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Merrin, Jack","orcid":"0000-0001-5145-4609","last_name":"Merrin","first_name":"Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K","first_name":"Michael K","last_name":"Sixt"}],"pmid":1,"_id":"153","scopus_import":"1","publisher":"Academic Press","page":"79 - 91","quality_controlled":"1","abstract":[{"lang":"eng","text":"Cells migrating in multicellular organisms steadily traverse complex three-dimensional (3D) environments. To decipher the underlying cell biology, current experimental setups either use simplified 2D, tissue-mimetic 3D (e.g., collagen matrices) or in vivo environments. While only in vivo experiments are truly physiological, they do not allow for precise manipulation of environmental parameters. 2D in vitro experiments do allow mechanical and chemical manipulations, but increasing evidence demonstrates substantial differences of migratory mechanisms in 2D and 3D. Here, we describe simple, robust, and versatile “pillar forests” to investigate cell migration in complex but fully controllable 3D environments. Pillar forests are polydimethylsiloxane-based setups, in which two closely adjacent surfaces are interconnected by arrays of micrometer-sized pillars. Changing the pillar shape, size, height and the inter-pillar distance precisely manipulates microenvironmental parameters (e.g., pore sizes, micro-geometry, micro-topology), while being easily combined with chemotactic cues, surface coatings, diverse cell types and advanced imaging techniques. Thus, pillar forests combine the advantages of 2D cell migration assays with the precise definition of 3D environmental parameters."}],"doi":"10.1016/bs.mcb.2018.07.004","day":"27","isi":1,"external_id":{"pmid":["30165964"],"isi":["000452412300006"]},"date_updated":"2023-09-13T08:56:35Z","year":"2018","citation":{"mla":"Renkawitz, Jörg, et al. “Micro-Engineered ‘Pillar Forests’ to Study Cell Migration in Complex but Controlled 3D Environments.” <i>Methods in Cell Biology</i>, vol. 147, Academic Press, 2018, pp. 79–91, doi:<a href=\"https://doi.org/10.1016/bs.mcb.2018.07.004\">10.1016/bs.mcb.2018.07.004</a>.","short":"J. Renkawitz, A. Reversat, A.F. Leithner, J. Merrin, M.K. Sixt, in:, Methods in Cell Biology, Academic Press, 2018, pp. 79–91.","ista":"Renkawitz J, Reversat A, Leithner AF, Merrin J, Sixt MK. 2018.Micro-engineered “pillar forests” to study cell migration in complex but controlled 3D environments. In: Methods in Cell Biology. vol. 147, 79–91.","apa":"Renkawitz, J., Reversat, A., Leithner, A. F., Merrin, J., &#38; Sixt, M. K. (2018). Micro-engineered “pillar forests” to study cell migration in complex but controlled 3D environments. In <i>Methods in Cell Biology</i> (Vol. 147, pp. 79–91). Academic Press. <a href=\"https://doi.org/10.1016/bs.mcb.2018.07.004\">https://doi.org/10.1016/bs.mcb.2018.07.004</a>","ama":"Renkawitz J, Reversat A, Leithner AF, Merrin J, Sixt MK. Micro-engineered “pillar forests” to study cell migration in complex but controlled 3D environments. In: <i>Methods in Cell Biology</i>. Vol 147. Academic Press; 2018:79-91. doi:<a href=\"https://doi.org/10.1016/bs.mcb.2018.07.004\">10.1016/bs.mcb.2018.07.004</a>","chicago":"Renkawitz, Jörg, Anne Reversat, Alexander F Leithner, Jack Merrin, and Michael K Sixt. “Micro-Engineered ‘Pillar Forests’ to Study Cell Migration in Complex but Controlled 3D Environments.” In <i>Methods in Cell Biology</i>, 147:79–91. Academic Press, 2018. <a href=\"https://doi.org/10.1016/bs.mcb.2018.07.004\">https://doi.org/10.1016/bs.mcb.2018.07.004</a>.","ieee":"J. Renkawitz, A. Reversat, A. F. Leithner, J. Merrin, and M. K. Sixt, “Micro-engineered ‘pillar forests’ to study cell migration in complex but controlled 3D environments,” in <i>Methods in Cell Biology</i>, vol. 147, Academic Press, 2018, pp. 79–91."},"volume":147,"month":"07","oa_version":"None","publication":"Methods in Cell Biology","language":[{"iso":"eng"}],"publist_id":"7768","publication_identifier":{"issn":["0091679X"]},"date_published":"2018-07-27T00:00:00Z","type":"book_chapter","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","status":"public"},{"publisher":"Cell Press","quality_controlled":"1","ec_funded":1,"page":"1314 - 1325","department":[{"_id":"MiSi"},{"_id":"Bio"},{"_id":"NanoFab"}],"date_created":"2018-12-11T11:47:51Z","publication_status":"published","intvolume":"        27","title":"Dendritic cells interpret haptotactic chemokine gradients in a manner governed by signal to noise ratio and dependent on GRK6","scopus_import":1,"_id":"674","issue":"9","author":[{"id":"346C1EC6-F248-11E8-B48F-1D18A9856A87","full_name":"Schwarz, Jan","last_name":"Schwarz","first_name":"Jan"},{"first_name":"Veronika","last_name":"Bierbaum","full_name":"Bierbaum, Veronika","id":"3FD04378-F248-11E8-B48F-1D18A9856A87"},{"id":"368EE576-F248-11E8-B48F-1D18A9856A87","first_name":"Kari","last_name":"Vaahtomeri","orcid":"0000-0001-7829-3518","full_name":"Vaahtomeri, Kari"},{"id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild","first_name":"Robert","full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522"},{"full_name":"Brown, Markus","last_name":"Brown","first_name":"Markus","id":"3DAB9AFC-F248-11E8-B48F-1D18A9856A87"},{"id":"4C7D837E-F248-11E8-B48F-1D18A9856A87","first_name":"Ingrid","last_name":"De Vries","full_name":"De Vries, Ingrid"},{"id":"3B1B77E4-F248-11E8-B48F-1D18A9856A87","last_name":"Leithner","first_name":"Alexander F","full_name":"Leithner, Alexander F"},{"id":"35B76592-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-0666-8928","full_name":"Reversat, Anne","first_name":"Anne","last_name":"Reversat"},{"full_name":"Merrin, Jack","orcid":"0000-0001-5145-4609","last_name":"Merrin","first_name":"Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Tarrant, Teresa","first_name":"Teresa","last_name":"Tarrant"},{"last_name":"Bollenbach","first_name":"Tobias","full_name":"Bollenbach, Tobias","orcid":"0000-0003-4398-476X","id":"3E6DB97A-F248-11E8-B48F-1D18A9856A87"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179","last_name":"Sixt","first_name":"Michael K"}],"volume":27,"day":"09","doi":"10.1016/j.cub.2017.04.004","abstract":[{"text":"Navigation of cells along gradients of guidance cues is a determining step in many developmental and immunological processes. Gradients can either be soluble or immobilized to tissues as demonstrated for the haptotactic migration of dendritic cells (DCs) toward higher concentrations of immobilized chemokine CCL21. To elucidate how gradient characteristics govern cellular response patterns, we here introduce an in vitro system allowing to track migratory responses of DCs to precisely controlled immobilized gradients of CCL21. We find that haptotactic sensing depends on the absolute CCL21 concentration and local steepness of the gradient, consistent with a scenario where DC directionality is governed by the signal-to-noise ratio of CCL21 binding to the receptor CCR7. We find that the conditions for optimal DC guidance are perfectly provided by the CCL21 gradients we measure in vivo. Furthermore, we find that CCR7 signal termination by the G-protein-coupled receptor kinase 6 (GRK6) is crucial for haptotactic but dispensable for chemotactic CCL21 gradient sensing in vitro and confirm those observations in vivo. These findings suggest that stable, tissue-bound CCL21 gradients as sustainable “roads” ensure optimal guidance in vivo.","lang":"eng"}],"year":"2017","citation":{"chicago":"Schwarz, Jan, Veronika Bierbaum, Kari Vaahtomeri, Robert Hauschild, Markus Brown, Ingrid de Vries, Alexander F Leithner, et al. “Dendritic Cells Interpret Haptotactic Chemokine Gradients in a Manner Governed by Signal to Noise Ratio and Dependent on GRK6.” <i>Current Biology</i>. Cell Press, 2017. <a href=\"https://doi.org/10.1016/j.cub.2017.04.004\">https://doi.org/10.1016/j.cub.2017.04.004</a>.","ieee":"J. Schwarz <i>et al.</i>, “Dendritic cells interpret haptotactic chemokine gradients in a manner governed by signal to noise ratio and dependent on GRK6,” <i>Current Biology</i>, vol. 27, no. 9. Cell Press, pp. 1314–1325, 2017.","ama":"Schwarz J, Bierbaum V, Vaahtomeri K, et al. Dendritic cells interpret haptotactic chemokine gradients in a manner governed by signal to noise ratio and dependent on GRK6. <i>Current Biology</i>. 2017;27(9):1314-1325. doi:<a href=\"https://doi.org/10.1016/j.cub.2017.04.004\">10.1016/j.cub.2017.04.004</a>","apa":"Schwarz, J., Bierbaum, V., Vaahtomeri, K., Hauschild, R., Brown, M., de Vries, I., … Sixt, M. K. (2017). Dendritic cells interpret haptotactic chemokine gradients in a manner governed by signal to noise ratio and dependent on GRK6. <i>Current Biology</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cub.2017.04.004\">https://doi.org/10.1016/j.cub.2017.04.004</a>","ista":"Schwarz J, Bierbaum V, Vaahtomeri K, Hauschild R, Brown M, de Vries I, Leithner AF, Reversat A, Merrin J, Tarrant T, Bollenbach MT, Sixt MK. 2017. Dendritic cells interpret haptotactic chemokine gradients in a manner governed by signal to noise ratio and dependent on GRK6. Current Biology. 27(9), 1314–1325.","short":"J. Schwarz, V. Bierbaum, K. Vaahtomeri, R. Hauschild, M. Brown, I. de Vries, A.F. Leithner, A. Reversat, J. Merrin, T. Tarrant, M.T. Bollenbach, M.K. Sixt, Current Biology 27 (2017) 1314–1325.","mla":"Schwarz, Jan, et al. “Dendritic Cells Interpret Haptotactic Chemokine Gradients in a Manner Governed by Signal to Noise Ratio and Dependent on GRK6.” <i>Current Biology</i>, vol. 27, no. 9, Cell Press, 2017, pp. 1314–25, doi:<a href=\"https://doi.org/10.1016/j.cub.2017.04.004\">10.1016/j.cub.2017.04.004</a>."},"date_updated":"2023-02-23T12:50:44Z","language":[{"iso":"eng"}],"project":[{"name":"International IST Postdoc Fellowship Programme","grant_number":"291734","_id":"25681D80-B435-11E9-9278-68D0E5697425","call_identifier":"FP7"},{"grant_number":"Y 564-B12","name":"Cytoskeletal force generation and transduction of leukocytes (FWF)","_id":"25A8E5EA-B435-11E9-9278-68D0E5697425","call_identifier":"FWF"}],"oa_version":"None","month":"05","publication":"Current Biology","status":"public","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","publication_identifier":{"issn":["09609822"]},"publist_id":"7050","type":"journal_article","date_published":"2017-05-09T00:00:00Z"},{"abstract":[{"text":"We report the enhancement of infrared absorption of chemisorbed carbon monoxide on platinum in the gap of plasmonic nanoantennas. Our method is based on the self-assembled formation of platinum nanoislands on nanoscopic dipole antenna arrays manufactured via electron beam lithography. We employ systematic variations of the plasmonic antenna resonance to precisely couple to the molecular stretch vibration of carbon monoxide adsorbed on the platinum nanoislands. Ultimately, we reach more than 1500-fold infrared absorption enhancements, allowing for an ultrasensitive detection of a monolayer of chemisorbed carbon monoxide. The developed procedure can be adapted to other metal adsorbents and molecular species and could be utilized for coverage sensing in surface catalytic reactions. ","lang":"eng"}],"doi":"10.1364/OL.42.001931","day":"15","date_updated":"2023-10-17T12:16:02Z","citation":{"ista":"Haase J, Bagiante S, Sigg H, Van Bokhoven J. 2017. Surface enhanced infrared absorption of chemisorbed carbon monoxide using plasmonic nanoantennas. Optics Letters. 42(10), 1931–1934.","short":"J. Haase, S. Bagiante, H. Sigg, J. Van Bokhoven, Optics Letters 42 (2017) 1931–1934.","mla":"Haase, Johannes, et al. “Surface Enhanced Infrared Absorption of Chemisorbed Carbon Monoxide Using Plasmonic Nanoantennas.” <i>Optics Letters</i>, vol. 42, no. 10, Optica Publishing Group, 2017, pp. 1931–34, doi:<a href=\"https://doi.org/10.1364/OL.42.001931\">10.1364/OL.42.001931</a>.","ieee":"J. Haase, S. Bagiante, H. Sigg, and J. Van Bokhoven, “Surface enhanced infrared absorption of chemisorbed carbon monoxide using plasmonic nanoantennas,” <i>Optics Letters</i>, vol. 42, no. 10. Optica Publishing Group, pp. 1931–1934, 2017.","chicago":"Haase, Johannes, Salvatore Bagiante, Hans Sigg, and Jeroen Van Bokhoven. “Surface Enhanced Infrared Absorption of Chemisorbed Carbon Monoxide Using Plasmonic Nanoantennas.” <i>Optics Letters</i>. Optica Publishing Group, 2017. <a href=\"https://doi.org/10.1364/OL.42.001931\">https://doi.org/10.1364/OL.42.001931</a>.","apa":"Haase, J., Bagiante, S., Sigg, H., &#38; Van Bokhoven, J. (2017). Surface enhanced infrared absorption of chemisorbed carbon monoxide using plasmonic nanoantennas. <i>Optics Letters</i>. Optica Publishing Group. <a href=\"https://doi.org/10.1364/OL.42.001931\">https://doi.org/10.1364/OL.42.001931</a>","ama":"Haase J, Bagiante S, Sigg H, Van Bokhoven J. Surface enhanced infrared absorption of chemisorbed carbon monoxide using plasmonic nanoantennas. <i>Optics Letters</i>. 2017;42(10):1931-1934. doi:<a href=\"https://doi.org/10.1364/OL.42.001931\">10.1364/OL.42.001931</a>"},"year":"2017","ddc":["530"],"volume":42,"title":"Surface enhanced infrared absorption of chemisorbed carbon monoxide using plasmonic nanoantennas","intvolume":"        42","publication_status":"published","article_processing_charge":"No","department":[{"_id":"NanoFab"}],"date_created":"2018-12-11T11:47:51Z","author":[{"last_name":"Haase","first_name":"Johannes","full_name":"Haase, Johannes"},{"orcid":"0000-0002-0122-9603","full_name":"Bagiante, Salvatore","first_name":"Salvatore","last_name":"Bagiante","id":"38ED402E-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Sigg, Hans","last_name":"Sigg","first_name":"Hans"},{"full_name":"Van Bokhoven, Jeroen","last_name":"Van Bokhoven","first_name":"Jeroen"}],"issue":"10","_id":"675","scopus_import":"1","article_type":"original","publisher":"Optica Publishing Group","page":"1931 - 1934","quality_controlled":"1","publist_id":"7048","date_published":"2017-05-15T00:00:00Z","type":"journal_article","status":"public","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","month":"05","oa_version":"None","publication":"Optics Letters","language":[{"iso":"eng"}]},{"type":"journal_article","date_published":"2017-05-05T00:00:00Z","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","image":"/images/cc_by_nc_nd.png"},"oa":1,"publist_id":"6412","publication_identifier":{"issn":["15306984"]},"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","status":"public","file":[{"access_level":"open_access","relation":"main_file","creator":"system","file_id":"5037","file_size":508638,"checksum":"22021daa90cf13b01becd776838acb7b","date_created":"2018-12-12T10:13:50Z","content_type":"application/pdf","file_name":"IST-2017-826-v1+1_2017_Aguilera-Servin_Current.pdf","date_updated":"2020-07-14T12:48:18Z"}],"has_accepted_license":"1","publication":"Nano Letters","month":"05","oa_version":"Published Version","language":[{"iso":"eng"}],"external_id":{"isi":["000403631600011"]},"isi":1,"citation":{"apa":"Nanda, G., Aguilera Servin, J. L., Rakyta, P., Kormányos, A., Kleiner, R., Koelle, D., … Goswami, S. (2017). Current-phase relation of ballistic graphene Josephson junctions. <i>Nano Letters</i>. American Chemical Society. <a href=\"https://doi.org/10.1021/acs.nanolett.7b00097\">https://doi.org/10.1021/acs.nanolett.7b00097</a>","ama":"Nanda G, Aguilera Servin JL, Rakyta P, et al. Current-phase relation of ballistic graphene Josephson junctions. <i>Nano Letters</i>. 2017;17(6):3396-3401. doi:<a href=\"https://doi.org/10.1021/acs.nanolett.7b00097\">10.1021/acs.nanolett.7b00097</a>","chicago":"Nanda, Gaurav, Juan L Aguilera Servin, Péter Rakyta, Andor Kormányos, Reinhold Kleiner, Dieter Koelle, Kazuo Watanabe, Takashi Taniguchi, Lieven Vandersypen, and Srijit Goswami. “Current-Phase Relation of Ballistic Graphene Josephson Junctions.” <i>Nano Letters</i>. American Chemical Society, 2017. <a href=\"https://doi.org/10.1021/acs.nanolett.7b00097\">https://doi.org/10.1021/acs.nanolett.7b00097</a>.","ieee":"G. Nanda <i>et al.</i>, “Current-phase relation of ballistic graphene Josephson junctions,” <i>Nano Letters</i>, vol. 17, no. 6. American Chemical Society, pp. 3396–3401, 2017.","mla":"Nanda, Gaurav, et al. “Current-Phase Relation of Ballistic Graphene Josephson Junctions.” <i>Nano Letters</i>, vol. 17, no. 6, American Chemical Society, 2017, pp. 3396–401, doi:<a href=\"https://doi.org/10.1021/acs.nanolett.7b00097\">10.1021/acs.nanolett.7b00097</a>.","short":"G. Nanda, J.L. Aguilera Servin, P. Rakyta, A. Kormányos, R. Kleiner, D. Koelle, K. Watanabe, T. Taniguchi, L. Vandersypen, S. Goswami, Nano Letters 17 (2017) 3396–3401.","ista":"Nanda G, Aguilera Servin JL, Rakyta P, Kormányos A, Kleiner R, Koelle D, Watanabe K, Taniguchi T, Vandersypen L, Goswami S. 2017. Current-phase relation of ballistic graphene Josephson junctions. Nano Letters. 17(6), 3396–3401."},"year":"2017","date_updated":"2023-09-22T09:56:21Z","abstract":[{"text":"The current-phase relation (CPR) of a Josephson junction (JJ) determines how the supercurrent evolves with the superconducting phase difference across the junction. Knowledge of the CPR is essential in order to understand the response of a JJ to various external parameters. Despite the rising interest in ultraclean encapsulated graphene JJs, the CPR of such junctions remains unknown. Here, we use a fully gate-tunable graphene superconducting quantum intereference device (SQUID) to determine the CPR of ballistic graphene JJs. Each of the two JJs in the SQUID is made with graphene encapsulated in hexagonal boron nitride. By independently controlling the critical current of the JJs, we can operate the SQUID either in a symmetric or asymmetric configuration. The highly asymmetric SQUID allows us to phase-bias one of the JJs and thereby directly obtain its CPR. The CPR is found to be skewed, deviating significantly from a sinusoidal form. The skewness can be tuned with the gate voltage and oscillates in antiphase with Fabry-Pérot resistance oscillations of the ballistic graphene cavity. We compare our experiments with tight-binding calculations that include realistic graphene-superconductor interfaces and find a good qualitative agreement.","lang":"eng"}],"day":"05","doi":"10.1021/acs.nanolett.7b00097","ddc":["621"],"volume":17,"issue":"6","author":[{"full_name":"Nanda, Gaurav","last_name":"Nanda","first_name":"Gaurav"},{"id":"2A67C376-F248-11E8-B48F-1D18A9856A87","first_name":"Juan L","last_name":"Aguilera Servin","orcid":"0000-0002-2862-8372","full_name":"Aguilera Servin, Juan L"},{"last_name":"Rakyta","first_name":"Péter","full_name":"Rakyta, Péter"},{"full_name":"Kormányos, Andor","last_name":"Kormányos","first_name":"Andor"},{"full_name":"Kleiner, Reinhold","first_name":"Reinhold","last_name":"Kleiner"},{"first_name":"Dieter","last_name":"Koelle","full_name":"Koelle, Dieter"},{"full_name":"Watanabe, Kazuo","last_name":"Watanabe","first_name":"Kazuo"},{"first_name":"Takashi","last_name":"Taniguchi","full_name":"Taniguchi, Takashi"},{"full_name":"Vandersypen, Lieven","first_name":"Lieven","last_name":"Vandersypen"},{"full_name":"Goswami, Srijit","last_name":"Goswami","first_name":"Srijit"}],"scopus_import":"1","_id":"988","intvolume":"        17","pubrep_id":"826","title":"Current-phase relation of ballistic graphene Josephson junctions","department":[{"_id":"NanoFab"}],"date_created":"2018-12-11T11:49:33Z","article_processing_charge":"No","publication_status":"published","file_date_updated":"2020-07-14T12:48:18Z","quality_controlled":"1","page":"3396 - 3401","publisher":"American Chemical Society"},{"publisher":"Nature Publishing Group","file_date_updated":"2018-12-12T10:09:32Z","ec_funded":1,"quality_controlled":"1","intvolume":"         6","title":"A microfluidic device for measuring cell migration towards substrate bound and soluble chemokine gradients","pubrep_id":"744","date_created":"2018-12-11T11:50:27Z","department":[{"_id":"MiSi"},{"_id":"NanoFab"},{"_id":"Bio"},{"_id":"ToBo"}],"publication_status":"published","author":[{"first_name":"Jan","last_name":"Schwarz","full_name":"Schwarz, Jan","id":"346C1EC6-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Bierbaum, Veronika","last_name":"Bierbaum","first_name":"Veronika","id":"3FD04378-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Merrin","first_name":"Jack","full_name":"Merrin, Jack","orcid":"0000-0001-5145-4609","id":"4515C308-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Frank","first_name":"Tino","full_name":"Frank, Tino"},{"orcid":"0000-0001-9843-3522","full_name":"Hauschild, Robert","first_name":"Robert","last_name":"Hauschild","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Bollenbach, Mark Tobias","orcid":"0000-0003-4398-476X","last_name":"Bollenbach","first_name":"Mark Tobias","id":"3E6DB97A-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Tay","first_name":"Savaş","full_name":"Tay, Savaş"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K","last_name":"Sixt","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K"},{"id":"3C23B994-F248-11E8-B48F-1D18A9856A87","full_name":"Mehling, Matthias","orcid":"0000-0001-8599-1226","last_name":"Mehling","first_name":"Matthias"}],"scopus_import":1,"_id":"1154","ddc":["579"],"acknowledgement":"This work was supported by the Swiss National Science Foundation (Ambizione fellowship; PZ00P3-154733 to M.M.), the Swiss Multiple Sclerosis Society (research support to M.M.), a fellowship from the Boehringer Ingelheim Fonds (BIF) to J.S., the European Research Council (grant ERC GA 281556) and a START award from the Austrian Science Foundation (FWF) to M.S. #BioimagingFacility","volume":6,"abstract":[{"lang":"eng","text":"Cellular locomotion is a central hallmark of eukaryotic life. It is governed by cell-extrinsic molecular factors, which can either emerge in the soluble phase or as immobilized, often adhesive ligands. To encode for direction, every cue must be present as a spatial or temporal gradient. Here, we developed a microfluidic chamber that allows measurement of cell migration in combined response to surface immobilized and soluble molecular gradients. As a proof of principle we study the response of dendritic cells to their major guidance cues, chemokines. The majority of data on chemokine gradient sensing is based on in vitro studies employing soluble gradients. Despite evidence suggesting that in vivo chemokines are often immobilized to sugar residues, limited information is available how cells respond to immobilized chemokines. We tracked migration of dendritic cells towards immobilized gradients of the chemokine CCL21 and varying superimposed soluble gradients of CCL19. Differential migratory patterns illustrate the potential of our setup to quantitatively study the competitive response to both types of gradients. Beyond chemokines our approach is broadly applicable to alternative systems of chemo- and haptotaxis such as cells migrating along gradients of adhesion receptor ligands vs. any soluble cue. \r\n"}],"day":"07","doi":"10.1038/srep36440","citation":{"short":"J. Schwarz, V. Bierbaum, J. Merrin, T. Frank, R. Hauschild, M.T. Bollenbach, S. Tay, M.K. Sixt, M. Mehling, Scientific Reports 6 (2016).","mla":"Schwarz, Jan, et al. “A Microfluidic Device for Measuring Cell Migration towards Substrate Bound and Soluble Chemokine Gradients.” <i>Scientific Reports</i>, vol. 6, 36440, Nature Publishing Group, 2016, doi:<a href=\"https://doi.org/10.1038/srep36440\">10.1038/srep36440</a>.","ista":"Schwarz J, Bierbaum V, Merrin J, Frank T, Hauschild R, Bollenbach MT, Tay S, Sixt MK, Mehling M. 2016. A microfluidic device for measuring cell migration towards substrate bound and soluble chemokine gradients. Scientific Reports. 6, 36440.","ama":"Schwarz J, Bierbaum V, Merrin J, et al. A microfluidic device for measuring cell migration towards substrate bound and soluble chemokine gradients. <i>Scientific Reports</i>. 2016;6. doi:<a href=\"https://doi.org/10.1038/srep36440\">10.1038/srep36440</a>","apa":"Schwarz, J., Bierbaum, V., Merrin, J., Frank, T., Hauschild, R., Bollenbach, M. T., … Mehling, M. (2016). A microfluidic device for measuring cell migration towards substrate bound and soluble chemokine gradients. <i>Scientific Reports</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/srep36440\">https://doi.org/10.1038/srep36440</a>","chicago":"Schwarz, Jan, Veronika Bierbaum, Jack Merrin, Tino Frank, Robert Hauschild, Mark Tobias Bollenbach, Savaş Tay, Michael K Sixt, and Matthias Mehling. “A Microfluidic Device for Measuring Cell Migration towards Substrate Bound and Soluble Chemokine Gradients.” <i>Scientific Reports</i>. Nature Publishing Group, 2016. <a href=\"https://doi.org/10.1038/srep36440\">https://doi.org/10.1038/srep36440</a>.","ieee":"J. Schwarz <i>et al.</i>, “A microfluidic device for measuring cell migration towards substrate bound and soluble chemokine gradients,” <i>Scientific Reports</i>, vol. 6. Nature Publishing Group, 2016."},"year":"2016","date_updated":"2021-01-12T06:48:41Z","language":[{"iso":"eng"}],"article_number":"36440","month":"11","project":[{"name":"Cytoskeletal force generation and force transduction of migrating leukocytes (EU)","grant_number":"281556","_id":"25A603A2-B435-11E9-9278-68D0E5697425","call_identifier":"FP7"},{"_id":"25A8E5EA-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","grant_number":"Y 564-B12","name":"Cytoskeletal force generation and transduction of leukocytes (FWF)"}],"oa_version":"Published Version","has_accepted_license":"1","publication":"Scientific Reports","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","status":"public","file":[{"date_updated":"2018-12-12T10:09:32Z","content_type":"application/pdf","file_name":"IST-2017-744-v1+1_srep36440.pdf","date_created":"2018-12-12T10:09:32Z","file_size":2353456,"file_id":"4756","creator":"system","relation":"main_file","access_level":"open_access"}],"oa":1,"publist_id":"6204","type":"journal_article","date_published":"2016-11-07T00:00:00Z","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"}},{"publication_status":"published","date_created":"2018-12-11T11:51:21Z","department":[{"_id":"MiSi"},{"_id":"NanoFab"},{"_id":"Bio"}],"article_processing_charge":"No","title":"Diversified actin protrusions promote environmental exploration but are dispensable for locomotion of leukocytes","intvolume":"        18","_id":"1321","scopus_import":1,"author":[{"full_name":"Leithner, Alexander F","orcid":"0000-0002-1073-744X","last_name":"Leithner","first_name":"Alexander F","id":"3B1B77E4-F248-11E8-B48F-1D18A9856A87"},{"id":"4DFA52AE-F248-11E8-B48F-1D18A9856A87","first_name":"Alexander","last_name":"Eichner","full_name":"Eichner, Alexander"},{"full_name":"Müller, Jan","first_name":"Jan","last_name":"Müller","id":"AD07FDB4-0F61-11EA-8158-C4CC64CEAA8D"},{"id":"35B76592-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-0666-8928","full_name":"Reversat, Anne","first_name":"Anne","last_name":"Reversat"},{"id":"3DAB9AFC-F248-11E8-B48F-1D18A9856A87","full_name":"Brown, Markus","last_name":"Brown","first_name":"Markus"},{"full_name":"Schwarz, Jan","last_name":"Schwarz","first_name":"Jan","id":"346C1EC6-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Merrin, Jack","orcid":"0000-0001-5145-4609","last_name":"Merrin","first_name":"Jack","id":"4515C308-F248-11E8-B48F-1D18A9856A87"},{"full_name":"De Gorter, David","last_name":"De Gorter","first_name":"David"},{"id":"48AD8942-F248-11E8-B48F-1D18A9856A87","last_name":"Schur","first_name":"Florian","full_name":"Schur, Florian","orcid":"0000-0003-4790-8078"},{"last_name":"Bayerl","first_name":"Jonathan","full_name":"Bayerl, Jonathan"},{"first_name":"Ingrid","last_name":"De Vries","full_name":"De Vries, Ingrid","id":"4C7D837E-F248-11E8-B48F-1D18A9856A87"},{"id":"355AA5A0-F248-11E8-B48F-1D18A9856A87","last_name":"Wieser","first_name":"Stefan","full_name":"Wieser, Stefan","orcid":"0000-0002-2670-2217"},{"first_name":"Robert","last_name":"Hauschild","orcid":"0000-0001-9843-3522","full_name":"Hauschild, Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Lai","first_name":"Frank","full_name":"Lai, Frank"},{"full_name":"Moser, Markus","last_name":"Moser","first_name":"Markus"},{"first_name":"Dontscho","last_name":"Kerjaschki","full_name":"Kerjaschki, Dontscho"},{"first_name":"Klemens","last_name":"Rottner","full_name":"Rottner, Klemens"},{"full_name":"Small, Victor","last_name":"Small","first_name":"Victor"},{"last_name":"Stradal","first_name":"Theresia","full_name":"Stradal, Theresia"},{"full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179","last_name":"Sixt","first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"}],"publisher":"Nature Publishing Group","article_type":"original","page":"1253 - 1259","quality_controlled":"1","ec_funded":1,"file_date_updated":"2020-07-14T12:44:43Z","doi":"10.1038/ncb3426","day":"24","abstract":[{"text":"Most migrating cells extrude their front by the force of actin polymerization. Polymerization requires an initial nucleation step, which is mediated by factors establishing either parallel filaments in the case of filopodia or branched filaments that form the branched lamellipodial network. Branches are considered essential for regular cell motility and are initiated by the Arp2/3 complex, which in turn is activated by nucleation-promoting factors of the WASP and WAVE families. Here we employed rapid amoeboid crawling leukocytes and found that deletion of the WAVE complex eliminated actin branching and thus lamellipodia formation. The cells were left with parallel filaments at the leading edge, which translated, depending on the differentiation status of the cell, into a unipolar pointed cell shape or cells with multiple filopodia. Remarkably, unipolar cells migrated with increased speed and enormous directional persistence, while they were unable to turn towards chemotactic gradients. Cells with multiple filopodia retained chemotactic activity but their migration was progressively impaired with increasing geometrical complexity of the extracellular environment. These findings establish that diversified leading edge protrusions serve as explorative structures while they slow down actual locomotion.","lang":"eng"}],"date_updated":"2024-03-25T23:30:09Z","citation":{"ieee":"A. F. Leithner <i>et al.</i>, “Diversified actin protrusions promote environmental exploration but are dispensable for locomotion of leukocytes,” <i>Nature Cell Biology</i>, vol. 18. Nature Publishing Group, pp. 1253–1259, 2016.","chicago":"Leithner, Alexander F, Alexander Eichner, Jan Müller, Anne Reversat, Markus Brown, Jan Schwarz, Jack Merrin, et al. “Diversified Actin Protrusions Promote Environmental Exploration but Are Dispensable for Locomotion of Leukocytes.” <i>Nature Cell Biology</i>. Nature Publishing Group, 2016. <a href=\"https://doi.org/10.1038/ncb3426\">https://doi.org/10.1038/ncb3426</a>.","ama":"Leithner AF, Eichner A, Müller J, et al. Diversified actin protrusions promote environmental exploration but are dispensable for locomotion of leukocytes. <i>Nature Cell Biology</i>. 2016;18:1253-1259. doi:<a href=\"https://doi.org/10.1038/ncb3426\">10.1038/ncb3426</a>","apa":"Leithner, A. F., Eichner, A., Müller, J., Reversat, A., Brown, M., Schwarz, J., … Sixt, M. K. (2016). Diversified actin protrusions promote environmental exploration but are dispensable for locomotion of leukocytes. <i>Nature Cell Biology</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/ncb3426\">https://doi.org/10.1038/ncb3426</a>","ista":"Leithner AF, Eichner A, Müller J, Reversat A, Brown M, Schwarz J, Merrin J, De Gorter D, Schur FK, Bayerl J, de Vries I, Wieser S, Hauschild R, Lai F, Moser M, Kerjaschki D, Rottner K, Small V, Stradal T, Sixt MK. 2016. Diversified actin protrusions promote environmental exploration but are dispensable for locomotion of leukocytes. Nature Cell Biology. 18, 1253–1259.","mla":"Leithner, Alexander F., et al. “Diversified Actin Protrusions Promote Environmental Exploration but Are Dispensable for Locomotion of Leukocytes.” <i>Nature Cell Biology</i>, vol. 18, Nature Publishing Group, 2016, pp. 1253–59, doi:<a href=\"https://doi.org/10.1038/ncb3426\">10.1038/ncb3426</a>.","short":"A.F. Leithner, A. Eichner, J. Müller, A. Reversat, M. Brown, J. Schwarz, J. Merrin, D. De Gorter, F.K. Schur, J. Bayerl, I. de Vries, S. Wieser, R. Hauschild, F. Lai, M. Moser, D. Kerjaschki, K. Rottner, V. Small, T. Stradal, M.K. Sixt, Nature Cell Biology 18 (2016) 1253–1259."},"year":"2016","volume":18,"acknowledgement":"This work was supported by the German Research Foundation (DFG) Priority Program SP 1464 to T.E.B.S. and M.S., and European Research Council (ERC GA 281556) and Human Frontiers Program grants to M.S.\r\nService Units of IST Austria for excellent technical support.","ddc":["570"],"acknowledged_ssus":[{"_id":"SSU"}],"oa_version":"Submitted Version","project":[{"call_identifier":"FP7","_id":"25A603A2-B435-11E9-9278-68D0E5697425","name":"Cytoskeletal force generation and force transduction of migrating leukocytes (EU)","grant_number":"281556"}],"month":"10","publication":"Nature Cell Biology","has_accepted_license":"1","language":[{"iso":"eng"}],"oa":1,"publist_id":"5949","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)","image":"/images/cc_by_nc_sa.png","short":"CC BY-NC-SA (4.0)"},"date_published":"2016-10-24T00:00:00Z","type":"journal_article","file":[{"content_type":"application/pdf","file_name":"2018_NatureCell_Leithner.pdf","date_updated":"2020-07-14T12:44:43Z","file_size":4433280,"checksum":"e1411cb7c99a2d9089c178a6abef25e7","date_created":"2020-05-14T16:33:46Z","creator":"dernst","file_id":"7844","access_level":"open_access","relation":"main_file"}],"status":"public","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","related_material":{"record":[{"id":"323","relation":"dissertation_contains","status":"public"}]}}]