---
_id: '2882'
abstract:
- lang: eng
text: Gravitropic bending of plant organs is mediated by an asymmetric signaling
of the plant hormone auxin between the upper and lower side of the respective
organ. Here, we show that also another plant hormone, gibberellic acid (GA), shows
asymmetric action during gravitropic responses. Immunodetection using an antibody
against GA and monitoring GA signaling output by downstream degradation of DELLA
proteins revealed an asymmetric GA distribution and response with the maximum
at the lower side of gravistimulated roots. Genetic or pharmacological manipulation
of GA levels or response affects gravity-mediated auxin redistribution and root
bending response. The higher GA levels at the lower side of the root correlate
with increased amounts of PIN-FORMED2 (PIN2) auxin transporter at the plasma membrane.
The observed increase in PIN2 stability is caused by a specific GA effect on trafficking
of PIN proteins to lytic vacuoles that presumably occurs downstream of brefeldin
A-sensitive endosomes. Our results suggest that asymmetric auxin distribution
instructive for gravity-induced differential growth is consolidated by the asymmetric
action of GA that stabilizes the PIN-dependent auxin stream along the lower side
of gravistimulated roots.
author:
- first_name: Christian
full_name: Löfke, Christian
last_name: Löfke
- first_name: Marta
full_name: Zwiewka, Marta
last_name: Zwiewka
- first_name: Ingo
full_name: Heilmann, Ingo
last_name: Heilmann
- first_name: Marc
full_name: Van Montagu, Marc
last_name: Van Montagu
- first_name: Thomas
full_name: Teichmann, Thomas
last_name: Teichmann
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Löfke C, Zwiewka M, Heilmann I, Van Montagu M, Teichmann T, Friml J. Asymmetric
gibberellin signaling regulates vacuolar trafficking of PIN auxin transporters
during root gravitropism. PNAS. 2013;110(9):3627-3632. doi:10.1073/pnas.1300107110
apa: Löfke, C., Zwiewka, M., Heilmann, I., Van Montagu, M., Teichmann, T., &
Friml, J. (2013). Asymmetric gibberellin signaling regulates vacuolar trafficking
of PIN auxin transporters during root gravitropism. PNAS. National Academy
of Sciences. https://doi.org/10.1073/pnas.1300107110
chicago: Löfke, Christian, Marta Zwiewka, Ingo Heilmann, Marc Van Montagu, Thomas
Teichmann, and Jiří Friml. “Asymmetric Gibberellin Signaling Regulates Vacuolar
Trafficking of PIN Auxin Transporters during Root Gravitropism.” PNAS.
National Academy of Sciences, 2013. https://doi.org/10.1073/pnas.1300107110.
ieee: C. Löfke, M. Zwiewka, I. Heilmann, M. Van Montagu, T. Teichmann, and J. Friml,
“Asymmetric gibberellin signaling regulates vacuolar trafficking of PIN auxin
transporters during root gravitropism,” PNAS, vol. 110, no. 9. National
Academy of Sciences, pp. 3627–3632, 2013.
ista: Löfke C, Zwiewka M, Heilmann I, Van Montagu M, Teichmann T, Friml J. 2013.
Asymmetric gibberellin signaling regulates vacuolar trafficking of PIN auxin transporters
during root gravitropism. PNAS. 110(9), 3627–3632.
mla: Löfke, Christian, et al. “Asymmetric Gibberellin Signaling Regulates Vacuolar
Trafficking of PIN Auxin Transporters during Root Gravitropism.” PNAS,
vol. 110, no. 9, National Academy of Sciences, 2013, pp. 3627–32, doi:10.1073/pnas.1300107110.
short: C. Löfke, M. Zwiewka, I. Heilmann, M. Van Montagu, T. Teichmann, J. Friml,
PNAS 110 (2013) 3627–3632.
date_created: 2018-12-11T12:00:07Z
date_published: 2013-02-26T00:00:00Z
date_updated: 2021-01-12T07:00:27Z
day: '26'
department:
- _id: JiFr
doi: 10.1073/pnas.1300107110
external_id:
pmid:
- '23391733'
intvolume: ' 110'
issue: '9'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3587205/
month: '02'
oa: 1
oa_version: Submitted Version
page: 3627 - 3632
pmid: 1
publication: PNAS
publication_status: published
publisher: National Academy of Sciences
publist_id: '3879'
quality_controlled: '1'
scopus_import: 1
status: public
title: Asymmetric gibberellin signaling regulates vacuolar trafficking of PIN auxin
transporters during root gravitropism
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 110
year: '2013'
...
---
_id: '2883'
abstract:
- lang: eng
text: Plant architecture is influenced by the polar, cell-to-cell transport of auxin
that is primarily provided and regulated by plasma membrane efflux catalysts of
the PIN-FORMED and B family of ABC transporter (ABCB) classes. The latter were
shown to require the functionality of the FK506 binding protein42 TWISTED DWARF1
(TWD1), although underlying mechanisms are unclear. By genetic manipulation of
TWD1 expression, we show here that TWD1 affects shootward root auxin reflux and,
thus, downstream developmental traits, such as epidermal twisting and gravitropism
of the root. Using immunological assays, we demonstrate a predominant lateral,
mainly outward-facing, plasma membrane location for TWD1 in the root epidermis
characterized by the lateral marker ABC transporter G36/PLEIOTROPIC DRUG-RESISTANCE8/PENETRATION3.
At these epidermal plasma membrane domains, TWD1 colocalizes with nonpolar ABCB1.
In planta bioluminescence resonance energy transfer analysis was used to verify
specific ABC transporter B1 (ABCB1)-TWD1 interaction. Our data support a model
in which TWD1 promotes lateral ABCB-mediated auxin efflux via protein-protein
interaction at the plasma membrane, minimizing reflux from the root apoplast into
the cytoplasm.
acknowledgement: We would thank Vincent Vincenzetti and Laurence Charrier for excellent
technical assistance, A. von Arnim for the donation of BRET vectors, E. Spalding
for TWD1-CFP, TWD1-CFP/29-1-GFP/ER-YFP, and ABCB4-GFP lines, M. Palmgren for discussion
and support, and E. Martinoia for TT12 cDNA, support, and mentorship. Imaging data
were partially collected at the Center for Advanced Bioimaging, University of Copenhagen,
Denmark. This work was supported by grants from the Novartis Foundation (to M.G.),
from the Danish Research School for Biotechnology (to M.G. and A.S.), from the Forschungskredit
of the University of Zurich (to A.B.), from the Pool de Recherche of the University
of Fribourg (to M.G.), and from the Swiss National Funds (to M.G.). M.G. dedicates
this work to his father, who passed away during the resubmission process.
author:
- first_name: Bangjun
full_name: Wang, Bangjun
last_name: Wang
- first_name: Aurélien
full_name: Bailly, Aurélien
last_name: Bailly
- first_name: Marta
full_name: Zwiewk, Marta
last_name: Zwiewk
- first_name: Sina
full_name: Henrichs, Sina
last_name: Henrichs
- first_name: Elisa
full_name: Azzarello, Elisa
last_name: Azzarello
- first_name: Stefano
full_name: Mancuso, Stefano
last_name: Mancuso
- first_name: Masayoshi
full_name: Maeshima, Masayoshi
last_name: Maeshima
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Alexander
full_name: Schulz, Alexander
last_name: Schulz
- first_name: Markus
full_name: Geisler, Markus
last_name: Geisler
citation:
ama: Wang B, Bailly A, Zwiewk M, et al. Arabidopsis TWISTED DWARF1 functionally
interacts with auxin exporter ABCB1 on the root plasma membrane. Plant Cell.
2013;25(1):202-214. doi:10.1105/tpc.112.105999
apa: Wang, B., Bailly, A., Zwiewk, M., Henrichs, S., Azzarello, E., Mancuso, S.,
… Geisler, M. (2013). Arabidopsis TWISTED DWARF1 functionally interacts with auxin
exporter ABCB1 on the root plasma membrane. Plant Cell. American Society
of Plant Biologists. https://doi.org/10.1105/tpc.112.105999
chicago: Wang, Bangjun, Aurélien Bailly, Marta Zwiewk, Sina Henrichs, Elisa Azzarello,
Stefano Mancuso, Masayoshi Maeshima, Jiří Friml, Alexander Schulz, and Markus
Geisler. “Arabidopsis TWISTED DWARF1 Functionally Interacts with Auxin Exporter
ABCB1 on the Root Plasma Membrane.” Plant Cell. American Society of Plant
Biologists, 2013. https://doi.org/10.1105/tpc.112.105999.
ieee: B. Wang et al., “Arabidopsis TWISTED DWARF1 functionally interacts
with auxin exporter ABCB1 on the root plasma membrane,” Plant Cell, vol.
25, no. 1. American Society of Plant Biologists, pp. 202–214, 2013.
ista: Wang B, Bailly A, Zwiewk M, Henrichs S, Azzarello E, Mancuso S, Maeshima M,
Friml J, Schulz A, Geisler M. 2013. Arabidopsis TWISTED DWARF1 functionally interacts
with auxin exporter ABCB1 on the root plasma membrane. Plant Cell. 25(1), 202–214.
mla: Wang, Bangjun, et al. “Arabidopsis TWISTED DWARF1 Functionally Interacts with
Auxin Exporter ABCB1 on the Root Plasma Membrane.” Plant Cell, vol. 25,
no. 1, American Society of Plant Biologists, 2013, pp. 202–14, doi:10.1105/tpc.112.105999.
short: B. Wang, A. Bailly, M. Zwiewk, S. Henrichs, E. Azzarello, S. Mancuso, M.
Maeshima, J. Friml, A. Schulz, M. Geisler, Plant Cell 25 (2013) 202–214.
date_created: 2018-12-11T12:00:08Z
date_published: 2013-01-01T00:00:00Z
date_updated: 2021-01-12T07:00:28Z
day: '01'
department:
- _id: JiFr
doi: 10.1105/tpc.112.105999
external_id:
pmid:
- '23321285'
intvolume: ' 25'
issue: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3584535/
month: '01'
oa: 1
oa_version: Submitted Version
page: 202 - 214
pmid: 1
publication: Plant Cell
publication_status: published
publisher: American Society of Plant Biologists
publist_id: '3878'
quality_controlled: '1'
scopus_import: 1
status: public
title: Arabidopsis TWISTED DWARF1 functionally interacts with auxin exporter ABCB1
on the root plasma membrane
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 25
year: '2013'
...
---
_id: '2919'
abstract:
- lang: eng
text: The distribution of the phytohormone auxin regulates many aspects of plant
development including growth response to gravity. Gravitropic root curvature involves
coordinated and asymmetric cell elongation between the lower and upper side of
the root, mediated by differential cellular auxin levels. The asymmetry in the
auxin distribution is established and maintained by a spatio-temporal regulation
of the PIN-FORMED (PIN) auxin transporter activity. We provide novel insights
into the complex regulation of PIN abundance and activity during root gravitropism.
We show that PIN2 turnover is differentially regulated on the upper and lower
side of gravistimulated roots by distinct but partially overlapping auxin feedback
mechanisms. In addition to regulating transcription and clathrin-mediated internalization,
auxin also controls PIN abundance at the plasma membrane by promoting their vacuolar
targeting and degradation. This effect of elevated auxin levels requires the activity
of SKP-Cullin-F-box TIR1/AFB (SCF TIR1/AFB)-dependent pathway. Importantly, also
suboptimal auxin levels mediate PIN degradation utilizing the same signalling
pathway. These feedback mechanisms are functionally important during gravitropic
response and ensure fine-tuning of auxin fluxes for maintaining as well as terminating
asymmetric growth.
author:
- first_name: Pawel
full_name: Baster, Pawel
id: 3028BD74-F248-11E8-B48F-1D18A9856A87
last_name: Baster
- first_name: Stéphanie
full_name: Robert, Stéphanie
last_name: Robert
- first_name: Jürgen
full_name: Kleine Vehn, Jürgen
last_name: Kleine Vehn
- first_name: Steffen
full_name: Vanneste, Steffen
last_name: Vanneste
- first_name: Urszula
full_name: Kania, Urszula
id: 4AE5C486-F248-11E8-B48F-1D18A9856A87
last_name: Kania
- first_name: Wim
full_name: Grunewald, Wim
last_name: Grunewald
- first_name: Bert
full_name: De Rybel, Bert
last_name: De Rybel
- first_name: Tom
full_name: Beeckman, Tom
last_name: Beeckman
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Baster P, Robert S, Kleine Vehn J, et al. SCF^TIR1 AFB-auxin signalling regulates
PIN vacuolar trafficking and auxin fluxes during root gravitropism. EMBO Journal.
2013;32(2):260-274. doi:10.1038/emboj.2012.310
apa: Baster, P., Robert, S., Kleine Vehn, J., Vanneste, S., Kania, U., Grunewald,
W., … Friml, J. (2013). SCF^TIR1 AFB-auxin signalling regulates PIN vacuolar trafficking
and auxin fluxes during root gravitropism. EMBO Journal. Wiley-Blackwell.
https://doi.org/10.1038/emboj.2012.310
chicago: Baster, Pawel, Stéphanie Robert, Jürgen Kleine Vehn, Steffen Vanneste,
Urszula Kania, Wim Grunewald, Bert De Rybel, Tom Beeckman, and Jiří Friml. “SCF^TIR1
AFB-Auxin Signalling Regulates PIN Vacuolar Trafficking and Auxin Fluxes during
Root Gravitropism.” EMBO Journal. Wiley-Blackwell, 2013. https://doi.org/10.1038/emboj.2012.310.
ieee: P. Baster et al., “SCF^TIR1 AFB-auxin signalling regulates PIN vacuolar
trafficking and auxin fluxes during root gravitropism,” EMBO Journal, vol.
32, no. 2. Wiley-Blackwell, pp. 260–274, 2013.
ista: Baster P, Robert S, Kleine Vehn J, Vanneste S, Kania U, Grunewald W, De Rybel
B, Beeckman T, Friml J. 2013. SCF^TIR1 AFB-auxin signalling regulates PIN vacuolar
trafficking and auxin fluxes during root gravitropism. EMBO Journal. 32(2), 260–274.
mla: Baster, Pawel, et al. “SCF^TIR1 AFB-Auxin Signalling Regulates PIN Vacuolar
Trafficking and Auxin Fluxes during Root Gravitropism.” EMBO Journal, vol.
32, no. 2, Wiley-Blackwell, 2013, pp. 260–74, doi:10.1038/emboj.2012.310.
short: P. Baster, S. Robert, J. Kleine Vehn, S. Vanneste, U. Kania, W. Grunewald,
B. De Rybel, T. Beeckman, J. Friml, EMBO Journal 32 (2013) 260–274.
date_created: 2018-12-11T12:00:20Z
date_published: 2013-01-23T00:00:00Z
date_updated: 2021-01-12T07:00:41Z
day: '23'
department:
- _id: JiFr
doi: 10.1038/emboj.2012.310
external_id:
pmid:
- '23211744'
intvolume: ' 32'
issue: '2'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3553380/
month: '01'
oa: 1
oa_version: Submitted Version
page: 260 - 274
pmid: 1
publication: EMBO Journal
publication_status: published
publisher: Wiley-Blackwell
publist_id: '3818'
quality_controlled: '1'
scopus_import: 1
status: public
title: SCF^TIR1 AFB-auxin signalling regulates PIN vacuolar trafficking and auxin
fluxes during root gravitropism
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 32
year: '2013'
...
---
_id: '10895'
abstract:
- lang: eng
text: 'Due to their sessile lifestyles, plants need to deal with the limitations
and stresses imposed by the changing environment. Plants cope with these by a
remarkable developmental flexibility, which is embedded in their strategy to survive.
Plants can adjust their size, shape and number of organs, bend according to gravity
and light, and regenerate tissues that were damaged, utilizing a coordinating,
intercellular signal, the plant hormone, auxin. Another versatile signal is the
cation, Ca2+, which is a crucial second messenger for many rapid cellular processes
during responses to a wide range of endogenous and environmental signals, such
as hormones, light, drought stress and others. Auxin is a good candidate for one
of these Ca2+-activating signals. However, the role of auxin-induced Ca2+ signaling
is poorly understood. Here, we will provide an overview of possible developmental
and physiological roles, as well as mechanisms underlying the interconnection
of Ca2+ and auxin signaling. '
article_processing_charge: No
article_type: original
author:
- first_name: Steffen
full_name: Vanneste, Steffen
last_name: Vanneste
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: 'Vanneste S, Friml J. Calcium: The missing link in auxin action. Plants.
2013;2(4):650-675. doi:10.3390/plants2040650'
apa: 'Vanneste, S., & Friml, J. (2013). Calcium: The missing link in auxin action.
Plants. MDPI. https://doi.org/10.3390/plants2040650'
chicago: 'Vanneste, Steffen, and Jiří Friml. “Calcium: The Missing Link in Auxin
Action.” Plants. MDPI, 2013. https://doi.org/10.3390/plants2040650.'
ieee: 'S. Vanneste and J. Friml, “Calcium: The missing link in auxin action,” Plants,
vol. 2, no. 4. MDPI, pp. 650–675, 2013.'
ista: 'Vanneste S, Friml J. 2013. Calcium: The missing link in auxin action. Plants.
2(4), 650–675.'
mla: 'Vanneste, Steffen, and Jiří Friml. “Calcium: The Missing Link in Auxin Action.”
Plants, vol. 2, no. 4, MDPI, 2013, pp. 650–75, doi:10.3390/plants2040650.'
short: S. Vanneste, J. Friml, Plants 2 (2013) 650–675.
date_created: 2022-03-21T07:13:49Z
date_published: 2013-10-21T00:00:00Z
date_updated: 2022-03-21T12:15:29Z
day: '21'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.3390/plants2040650
external_id:
pmid:
- '27137397'
file:
- access_level: open_access
checksum: fb4ff2e820e344e253c9197544610be6
content_type: application/pdf
creator: dernst
date_created: 2022-03-21T12:12:56Z
date_updated: 2022-03-21T12:12:56Z
file_id: '10916'
file_name: 2013_Plants_Vanneste.pdf
file_size: 670188
relation: main_file
success: 1
file_date_updated: 2022-03-21T12:12:56Z
has_accepted_license: '1'
intvolume: ' 2'
issue: '4'
keyword:
- Plant Science
- Ecology
- Ecology
- Evolution
- Behavior and Systematics
language:
- iso: eng
license: https://creativecommons.org/licenses/by/3.0/
month: '10'
oa: 1
oa_version: Published Version
page: 650-675
pmid: 1
publication: Plants
publication_identifier:
issn:
- 2223-7747
publication_status: published
publisher: MDPI
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Calcium: The missing link in auxin action'
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/3.0/legalcode
name: Creative Commons Attribution 3.0 Unported (CC BY 3.0)
short: CC BY (3.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 2
year: '2013'
...
---
_id: '2290'
abstract:
- lang: eng
text: The plant hormone indole-acetic acid (auxin) is essential for many aspects
of plant development. Auxin-mediated growth regulation typically involves the
establishment of an auxin concentration gradient mediated by polarly localized
auxin transporters. The localization of auxin carriers and their amount at the
plasma membrane are controlled by membrane trafficking processes such as secretion,
endocytosis, and recycling. In contrast to endocytosis or recycling, how the secretory
pathway mediates the localization of auxin carriers is not well understood. In
this study we have used the differential cell elongation process during apical
hook development to elucidate the mechanisms underlying the post-Golgi trafficking
of auxin carriers in Arabidopsis. We show that differential cell elongation during
apical hook development is defective in Arabidopsis mutant echidna (ech). ECH
protein is required for the trans-Golgi network (TGN)-mediated trafficking of
the auxin influx carrier AUX1 to the plasma membrane. In contrast, ech mutation
only marginally perturbs the trafficking of the highly related auxin influx carrier
LIKE-AUX1-3 or the auxin efflux carrier PIN-FORMED-3, both also involved in hook
development. Electron tomography reveals that the trafficking defects in ech mutant
are associated with the perturbation of secretory vesicle genesis from the TGN.
Our results identify differential mechanisms for the post-Golgi trafficking of
de novo-synthesized auxin carriers to plasma membrane from the TGN and reveal
how trafficking of auxin influx carriers mediates the control of differential
cell elongation in apical hook development.
author:
- first_name: Yohann
full_name: Boutté, Yohann
last_name: Boutté
- first_name: Kristoffer
full_name: Jonsson, Kristoffer
last_name: Jonsson
- first_name: Heather
full_name: Mcfarlane, Heather
last_name: Mcfarlane
- first_name: Errin
full_name: Johnson, Errin
last_name: Johnson
- first_name: Delphine
full_name: Gendre, Delphine
last_name: Gendre
- first_name: Ranjan
full_name: Swarup, Ranjan
last_name: Swarup
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Lacey
full_name: Samuels, Lacey
last_name: Samuels
- first_name: Stéphanie
full_name: Robert, Stéphanie
last_name: Robert
- first_name: Rishikesh
full_name: Bhalerao, Rishikesh
last_name: Bhalerao
citation:
ama: Boutté Y, Jonsson K, Mcfarlane H, et al. ECHIDNA mediated post Golgi trafficking
of auxin carriers for differential cell elongation. PNAS. 2013;110(40):16259-16264.
doi:10.1073/pnas.1309057110
apa: Boutté, Y., Jonsson, K., Mcfarlane, H., Johnson, E., Gendre, D., Swarup, R.,
… Bhalerao, R. (2013). ECHIDNA mediated post Golgi trafficking of auxin carriers
for differential cell elongation. PNAS. National Academy of Sciences. https://doi.org/10.1073/pnas.1309057110
chicago: Boutté, Yohann, Kristoffer Jonsson, Heather Mcfarlane, Errin Johnson, Delphine
Gendre, Ranjan Swarup, Jiří Friml, Lacey Samuels, Stéphanie Robert, and Rishikesh
Bhalerao. “ECHIDNA Mediated Post Golgi Trafficking of Auxin Carriers for Differential
Cell Elongation.” PNAS. National Academy of Sciences, 2013. https://doi.org/10.1073/pnas.1309057110.
ieee: Y. Boutté et al., “ECHIDNA mediated post Golgi trafficking of auxin
carriers for differential cell elongation,” PNAS, vol. 110, no. 40. National
Academy of Sciences, pp. 16259–16264, 2013.
ista: Boutté Y, Jonsson K, Mcfarlane H, Johnson E, Gendre D, Swarup R, Friml J,
Samuels L, Robert S, Bhalerao R. 2013. ECHIDNA mediated post Golgi trafficking
of auxin carriers for differential cell elongation. PNAS. 110(40), 16259–16264.
mla: Boutté, Yohann, et al. “ECHIDNA Mediated Post Golgi Trafficking of Auxin Carriers
for Differential Cell Elongation.” PNAS, vol. 110, no. 40, National Academy
of Sciences, 2013, pp. 16259–64, doi:10.1073/pnas.1309057110.
short: Y. Boutté, K. Jonsson, H. Mcfarlane, E. Johnson, D. Gendre, R. Swarup, J.
Friml, L. Samuels, S. Robert, R. Bhalerao, PNAS 110 (2013) 16259–16264.
date_created: 2018-12-11T11:56:48Z
date_published: 2013-10-01T00:00:00Z
date_updated: 2021-01-12T06:56:33Z
day: '01'
department:
- _id: JiFr
doi: 10.1073/pnas.1309057110
external_id:
pmid:
- '24043780'
intvolume: ' 110'
issue: '40'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3791722/
month: '10'
oa: 1
oa_version: Submitted Version
page: 16259 - 16264
pmid: 1
publication: PNAS
publication_status: published
publisher: National Academy of Sciences
publist_id: '4639'
quality_controlled: '1'
scopus_import: 1
status: public
title: ECHIDNA mediated post Golgi trafficking of auxin carriers for differential
cell elongation
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 110
year: '2013'
...
---
_id: '507'
abstract:
- lang: eng
text: Fertilization in flowering plants requires the temporal and spatial coordination
of many developmental processes, including pollen production, anther dehiscence,
ovule production, and pollen tube elongation. However, it remains elusive as to
how this coordination occurs during reproduction. Here, we present evidence that
endocytosis, involving heterotetrameric adaptor protein complex 2 (AP-2), plays
a crucial role in fertilization. An Arabidopsis thaliana mutant ap2m displays
multiple defects in pollen production and viability, as well as elongation of
staminal filaments and pollen tubes, all of which are pivotal processes needed
for fertilization. Of these abnormalities, the defects in elongation of staminal
filaments and pollen tubes were partially rescued by exogenous auxin. Moreover,
DR5rev:GFP (for green fluorescent protein) expression was greatly reduced in filaments
and anthers in ap2m mutant plants. At the cellular level, ap2m mutants displayed
defects in both endocytosis of N-(3-triethylammonium-propyl)-4- (4-diethylaminophenylhexatrienyl)
pyridinium dibromide, a lypophilic dye used as an endocytosis marker, and polar
localization of auxin-efflux carrier PIN FORMED2 (PIN2) in the stamen filaments.
Moreover, these defects were phenocopied by treatment with Tyrphostin A23, an
inhibitor of endocytosis. Based on these results, we propose that AP-2-dependent
endocytosis plays a crucial role in coordinating the multiple developmental aspects
of male reproductive organs by modulating cellular auxin level through the regulation
of the amount and polarity of PINs.
author:
- first_name: Soo
full_name: Kim, Soo
last_name: Kim
- first_name: Zheng
full_name: Xu, Zheng
last_name: Xu
- first_name: Kyungyoung
full_name: Song, Kyungyoung
last_name: Song
- first_name: Dae
full_name: Kim, Dae
last_name: Kim
- first_name: Hyangju
full_name: Kang, Hyangju
last_name: Kang
- first_name: Ilka
full_name: Reichardt, Ilka
last_name: Reichardt
- first_name: Eun
full_name: Sohn, Eun
last_name: Sohn
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Gerd
full_name: Juergens, Gerd
last_name: Juergens
- first_name: Inhwan
full_name: Hwang, Inhwan
last_name: Hwang
citation:
ama: Kim S, Xu Z, Song K, et al. Adaptor protein complex 2-mediated endocytosis
is crucial for male reproductive organ development in arabidopsis. Plant Cell.
2013;25(8):2970-2985. doi:10.1105/tpc.113.114264
apa: Kim, S., Xu, Z., Song, K., Kim, D., Kang, H., Reichardt, I., … Hwang, I. (2013).
Adaptor protein complex 2-mediated endocytosis is crucial for male reproductive
organ development in arabidopsis. Plant Cell. American Society of Plant
Biologists. https://doi.org/10.1105/tpc.113.114264
chicago: Kim, Soo, Zheng Xu, Kyungyoung Song, Dae Kim, Hyangju Kang, Ilka Reichardt,
Eun Sohn, Jiří Friml, Gerd Juergens, and Inhwan Hwang. “Adaptor Protein Complex
2-Mediated Endocytosis Is Crucial for Male Reproductive Organ Development in Arabidopsis.”
Plant Cell. American Society of Plant Biologists, 2013. https://doi.org/10.1105/tpc.113.114264.
ieee: S. Kim et al., “Adaptor protein complex 2-mediated endocytosis is crucial
for male reproductive organ development in arabidopsis,” Plant Cell, vol.
25, no. 8. American Society of Plant Biologists, pp. 2970–2985, 2013.
ista: Kim S, Xu Z, Song K, Kim D, Kang H, Reichardt I, Sohn E, Friml J, Juergens
G, Hwang I. 2013. Adaptor protein complex 2-mediated endocytosis is crucial for
male reproductive organ development in arabidopsis. Plant Cell. 25(8), 2970–2985.
mla: Kim, Soo, et al. “Adaptor Protein Complex 2-Mediated Endocytosis Is Crucial
for Male Reproductive Organ Development in Arabidopsis.” Plant Cell, vol.
25, no. 8, American Society of Plant Biologists, 2013, pp. 2970–85, doi:10.1105/tpc.113.114264.
short: S. Kim, Z. Xu, K. Song, D. Kim, H. Kang, I. Reichardt, E. Sohn, J. Friml,
G. Juergens, I. Hwang, Plant Cell 25 (2013) 2970–2985.
date_created: 2018-12-11T11:46:52Z
date_published: 2013-08-01T00:00:00Z
date_updated: 2021-01-12T08:01:12Z
day: '01'
department:
- _id: JiFr
doi: 10.1105/tpc.113.114264
external_id:
pmid:
- '23975898'
intvolume: ' 25'
issue: '8'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3784592/
month: '08'
oa: 1
oa_version: Submitted Version
page: 2970 - 2985
pmid: 1
publication: Plant Cell
publication_status: published
publisher: American Society of Plant Biologists
publist_id: '7312'
quality_controlled: '1'
scopus_import: 1
status: public
title: Adaptor protein complex 2-mediated endocytosis is crucial for male reproductive
organ development in arabidopsis
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 25
year: '2013'
...
---
_id: '509'
abstract:
- lang: eng
text: 'Clathrin-mediated endocytosis (CME) regulates many aspects of plant development,
including hormone signaling and responses to environmental stresses. Despite the
importance of this process, the machinery that regulates CME in plants is largely
unknown. In mammals, the heterotetrameric ADAPTOR PROTEIN COMPLEX-2 (AP-2) is
required for the formation of clathrin-coated vesicles at the plasma membrane
(PM). Although the existence of AP-2 has been predicted in Arabidopsis thaliana,
the biochemistry and functionality of the complex is still uncharacterized. Here,
we identified all the subunits of the Arabidopsis AP-2 by tandem affinity purification
and found that one of the large AP-2 subunits, AP2A1, localized at the PM and
interacted with clathrin. Furthermore, endocytosis of the leucine-rich repeat
receptor kinase, BRASSINOSTEROID INSENSITIVE1 (BRI1), was shown to depend on AP-2.
Knockdown of the two Arabidopsis AP2A genes or overexpression of a dominant-negative
version of the medium AP-2 subunit, AP2M, impaired BRI1 endocytosis and enhanced
the brassinosteroid signaling. Our data reveal that the CME machinery in Arabidopsis
is evolutionarily conserved and that AP-2 functions in receptormediated endocytosis. '
author:
- first_name: Simone
full_name: Di Rubbo, Simone
last_name: Di Rubbo
- first_name: Niloufer
full_name: Irani, Niloufer
last_name: Irani
- first_name: Soo
full_name: Kim, Soo
last_name: Kim
- first_name: Zheng
full_name: Xu, Zheng
last_name: Xu
- first_name: Astrid
full_name: Gadeyne, Astrid
last_name: Gadeyne
- first_name: Wim
full_name: Dejonghe, Wim
last_name: Dejonghe
- first_name: Isabelle
full_name: Vanhoutte, Isabelle
last_name: Vanhoutte
- first_name: Geert
full_name: Persiau, Geert
last_name: Persiau
- first_name: Dominique
full_name: Eeckhout, Dominique
last_name: Eeckhout
- first_name: Sibu
full_name: Simon, Sibu
id: 4542EF9A-F248-11E8-B48F-1D18A9856A87
last_name: Simon
orcid: 0000-0002-1998-6741
- first_name: Kyungyoung
full_name: Song, Kyungyoung
last_name: Song
- first_name: Jürgen
full_name: Kleine Vehn, Jürgen
last_name: Kleine Vehn
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Geert
full_name: De Jaeger, Geert
last_name: De Jaeger
- first_name: Daniël
full_name: Van Damme, Daniël
last_name: Van Damme
- first_name: Inhwan
full_name: Hwang, Inhwan
last_name: Hwang
- first_name: Eugenia
full_name: Russinova, Eugenia
last_name: Russinova
citation:
ama: Di Rubbo S, Irani N, Kim S, et al. The clathrin adaptor complex AP-2 mediates
endocytosis of brassinosteroid INSENSITIVE1 in arabidopsis. Plant Cell.
2013;25(8):2986-2997. doi:10.1105/tpc.113.114058
apa: Di Rubbo, S., Irani, N., Kim, S., Xu, Z., Gadeyne, A., Dejonghe, W., … Russinova,
E. (2013). The clathrin adaptor complex AP-2 mediates endocytosis of brassinosteroid
INSENSITIVE1 in arabidopsis. Plant Cell. American Society of Plant Biologists.
https://doi.org/10.1105/tpc.113.114058
chicago: Di Rubbo, Simone, Niloufer Irani, Soo Kim, Zheng Xu, Astrid Gadeyne, Wim
Dejonghe, Isabelle Vanhoutte, et al. “The Clathrin Adaptor Complex AP-2 Mediates
Endocytosis of Brassinosteroid INSENSITIVE1 in Arabidopsis.” Plant Cell.
American Society of Plant Biologists, 2013. https://doi.org/10.1105/tpc.113.114058.
ieee: S. Di Rubbo et al., “The clathrin adaptor complex AP-2 mediates endocytosis
of brassinosteroid INSENSITIVE1 in arabidopsis,” Plant Cell, vol. 25, no.
8. American Society of Plant Biologists, pp. 2986–2997, 2013.
ista: Di Rubbo S, Irani N, Kim S, Xu Z, Gadeyne A, Dejonghe W, Vanhoutte I, Persiau
G, Eeckhout D, Simon S, Song K, Kleine Vehn J, Friml J, De Jaeger G, Van Damme
D, Hwang I, Russinova E. 2013. The clathrin adaptor complex AP-2 mediates endocytosis
of brassinosteroid INSENSITIVE1 in arabidopsis. Plant Cell. 25(8), 2986–2997.
mla: Di Rubbo, Simone, et al. “The Clathrin Adaptor Complex AP-2 Mediates Endocytosis
of Brassinosteroid INSENSITIVE1 in Arabidopsis.” Plant Cell, vol. 25, no.
8, American Society of Plant Biologists, 2013, pp. 2986–97, doi:10.1105/tpc.113.114058.
short: S. Di Rubbo, N. Irani, S. Kim, Z. Xu, A. Gadeyne, W. Dejonghe, I. Vanhoutte,
G. Persiau, D. Eeckhout, S. Simon, K. Song, J. Kleine Vehn, J. Friml, G. De Jaeger,
D. Van Damme, I. Hwang, E. Russinova, Plant Cell 25 (2013) 2986–2997.
date_created: 2018-12-11T11:46:52Z
date_published: 2013-08-01T00:00:00Z
date_updated: 2021-01-12T08:01:13Z
day: '01'
department:
- _id: JiFr
doi: 10.1105/tpc.113.114058
external_id:
pmid:
- '23975899'
intvolume: ' 25'
issue: '8'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3784593/
month: '08'
oa: 1
oa_version: Submitted Version
page: 2986 - 2997
pmid: 1
publication: Plant Cell
publication_status: published
publisher: American Society of Plant Biologists
publist_id: '7311'
quality_controlled: '1'
scopus_import: 1
status: public
title: The clathrin adaptor complex AP-2 mediates endocytosis of brassinosteroid INSENSITIVE1
in arabidopsis
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 25
year: '2013'
...
---
_id: '511'
abstract:
- lang: eng
text: The native auxin, indole-3-acetic acid (IAA), is a major regulator of plant
growth and development. Its nonuniform distribution between cells and tissues
underlies the spatiotemporal coordination of many developmental events and responses
to environmental stimuli. The regulation of auxin gradients and the formation
of auxin maxima/minima most likely involve the regulation of both metabolic and
transport processes. In this article, we have demonstrated that 2-oxindole-3-acetic
acid (oxIAA) is a major primary IAA catabolite formed in Arabidopsis thaliana
root tissues. OxIAA had little biological activity and was formed rapidly and
irreversibly in response to increases in auxin levels. We further showed that
there is cell type-specific regulation of oxIAA levels in the Arabidopsis root
apex. We propose that oxIAA is an important element in the regulation of output
from auxin gradients and, therefore, in the regulation of auxin homeostasis and
response mechanisms.
author:
- first_name: Aleš
full_name: Pěnčík, Aleš
last_name: Pěnčík
- first_name: Biljana
full_name: Simonovik, Biljana
last_name: Simonovik
- first_name: Sara
full_name: Petersson, Sara
last_name: Petersson
- first_name: Eva
full_name: Henyková, Eva
last_name: Henyková
- first_name: Sibu
full_name: Simon, Sibu
id: 4542EF9A-F248-11E8-B48F-1D18A9856A87
last_name: Simon
orcid: 0000-0002-1998-6741
- first_name: Kathleen
full_name: Greenham, Kathleen
last_name: Greenham
- first_name: Yi
full_name: Zhang, Yi
last_name: Zhang
- first_name: Mariusz
full_name: Kowalczyk, Mariusz
last_name: Kowalczyk
- first_name: Mark
full_name: Estelle, Mark
last_name: Estelle
- first_name: Eva
full_name: Zažímalová, Eva
last_name: Zažímalová
- first_name: Ondřej
full_name: Novák, Ondřej
last_name: Novák
- first_name: Göran
full_name: Sandberg, Göran
last_name: Sandberg
- first_name: Karin
full_name: Ljung, Karin
last_name: Ljung
citation:
ama: Pěnčík A, Simonovik B, Petersson S, et al. Regulation of auxin homeostasis
and gradients in Arabidopsis roots through the formation of the indole-3-acetic
acid catabolite 2-oxindole-3-acetic acid. Plant Cell. 2013;25(10):3858-3870.
doi:10.1105/tpc.113.114421
apa: Pěnčík, A., Simonovik, B., Petersson, S., Henyková, E., Simon, S., Greenham,
K., … Ljung, K. (2013). Regulation of auxin homeostasis and gradients in Arabidopsis
roots through the formation of the indole-3-acetic acid catabolite 2-oxindole-3-acetic
acid. Plant Cell. American Society of Plant Biologists. https://doi.org/10.1105/tpc.113.114421
chicago: Pěnčík, Aleš, Biljana Simonovik, Sara Petersson, Eva Henyková, Sibu Simon,
Kathleen Greenham, Yi Zhang, et al. “Regulation of Auxin Homeostasis and Gradients
in Arabidopsis Roots through the Formation of the Indole-3-Acetic Acid Catabolite
2-Oxindole-3-Acetic Acid.” Plant Cell. American Society of Plant Biologists,
2013. https://doi.org/10.1105/tpc.113.114421.
ieee: A. Pěnčík et al., “Regulation of auxin homeostasis and gradients in
Arabidopsis roots through the formation of the indole-3-acetic acid catabolite
2-oxindole-3-acetic acid,” Plant Cell, vol. 25, no. 10. American Society
of Plant Biologists, pp. 3858–3870, 2013.
ista: Pěnčík A, Simonovik B, Petersson S, Henyková E, Simon S, Greenham K, Zhang
Y, Kowalczyk M, Estelle M, Zažímalová E, Novák O, Sandberg G, Ljung K. 2013. Regulation
of auxin homeostasis and gradients in Arabidopsis roots through the formation
of the indole-3-acetic acid catabolite 2-oxindole-3-acetic acid. Plant Cell. 25(10),
3858–3870.
mla: Pěnčík, Aleš, et al. “Regulation of Auxin Homeostasis and Gradients in Arabidopsis
Roots through the Formation of the Indole-3-Acetic Acid Catabolite 2-Oxindole-3-Acetic
Acid.” Plant Cell, vol. 25, no. 10, American Society of Plant Biologists,
2013, pp. 3858–70, doi:10.1105/tpc.113.114421.
short: A. Pěnčík, B. Simonovik, S. Petersson, E. Henyková, S. Simon, K. Greenham,
Y. Zhang, M. Kowalczyk, M. Estelle, E. Zažímalová, O. Novák, G. Sandberg, K. Ljung,
Plant Cell 25 (2013) 3858–3870.
date_created: 2018-12-11T11:46:53Z
date_published: 2013-10-01T00:00:00Z
date_updated: 2021-01-12T08:01:15Z
day: '01'
department:
- _id: JiFr
doi: 10.1105/tpc.113.114421
external_id:
pmid:
- '24163311'
intvolume: ' 25'
issue: '10'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: www.doi.org/10.1105/tpc.113.114421
month: '10'
oa: 1
oa_version: Published Version
page: 3858 - 3870
pmid: 1
publication: Plant Cell
publication_status: published
publisher: American Society of Plant Biologists
publist_id: '7309'
quality_controlled: '1'
scopus_import: 1
status: public
title: Regulation of auxin homeostasis and gradients in Arabidopsis roots through
the formation of the indole-3-acetic acid catabolite 2-oxindole-3-acetic acid
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 25
year: '2013'
...
---
_id: '516'
abstract:
- lang: eng
text: In plants, changes in local auxin concentrations can trigger a range of developmental
processes as distinct tissues respond differently to the same auxin stimulus.
However, little is known about how auxin is interpreted by individual cell types.
We performed a transcriptomic analysis of responses to auxin within four distinct
tissues of the Arabidopsis thaliana root and demonstrate that different cell types
show competence for discrete responses. The majority of auxin‐responsive genes
displayed a spatial bias in their induction or repression. The novel data set
was used to examine how auxin influences tissue‐specific transcriptional regulation
of cell‐identity markers. Additionally, the data were used in combination with
spatial expression maps of the root to plot a transcriptomic auxin‐response gradient
across the apical and basal meristem. The readout revealed a strong correlation
for thousands of genes between the relative response to auxin and expression along
the longitudinal axis of the root. This data set and comparative analysis provide
a transcriptome‐level spatial breakdown of the response to auxin within an organ
where this hormone mediates many aspects of development.
article_number: '688'
article_processing_charge: No
author:
- first_name: Bastiaan
full_name: Bargmann, Bastiaan
last_name: Bargmann
- first_name: Steffen
full_name: Vanneste, Steffen
last_name: Vanneste
- first_name: Gabriel
full_name: Krouk, Gabriel
last_name: Krouk
- first_name: Tal
full_name: Nawy, Tal
last_name: Nawy
- first_name: Idan
full_name: Efroni, Idan
last_name: Efroni
- first_name: Eilon
full_name: Shani, Eilon
last_name: Shani
- first_name: Goh
full_name: Choe, Goh
last_name: Choe
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Dominique
full_name: Bergmann, Dominique
last_name: Bergmann
- first_name: Mark
full_name: Estelle, Mark
last_name: Estelle
- first_name: Kenneth
full_name: Birnbaum, Kenneth
last_name: Birnbaum
citation:
ama: Bargmann B, Vanneste S, Krouk G, et al. A map of cell type‐specific auxin responses.
Molecular Systems Biology. 2013;9(1). doi:10.1038/msb.2013.40
apa: Bargmann, B., Vanneste, S., Krouk, G., Nawy, T., Efroni, I., Shani, E., … Birnbaum,
K. (2013). A map of cell type‐specific auxin responses. Molecular Systems Biology.
Nature Publishing Group. https://doi.org/10.1038/msb.2013.40
chicago: Bargmann, Bastiaan, Steffen Vanneste, Gabriel Krouk, Tal Nawy, Idan Efroni,
Eilon Shani, Goh Choe, et al. “A Map of Cell Type‐specific Auxin Responses.” Molecular
Systems Biology. Nature Publishing Group, 2013. https://doi.org/10.1038/msb.2013.40.
ieee: B. Bargmann et al., “A map of cell type‐specific auxin responses,”
Molecular Systems Biology, vol. 9, no. 1. Nature Publishing Group, 2013.
ista: Bargmann B, Vanneste S, Krouk G, Nawy T, Efroni I, Shani E, Choe G, Friml
J, Bergmann D, Estelle M, Birnbaum K. 2013. A map of cell type‐specific auxin
responses. Molecular Systems Biology. 9(1), 688.
mla: Bargmann, Bastiaan, et al. “A Map of Cell Type‐specific Auxin Responses.” Molecular
Systems Biology, vol. 9, no. 1, 688, Nature Publishing Group, 2013, doi:10.1038/msb.2013.40.
short: B. Bargmann, S. Vanneste, G. Krouk, T. Nawy, I. Efroni, E. Shani, G. Choe,
J. Friml, D. Bergmann, M. Estelle, K. Birnbaum, Molecular Systems Biology 9 (2013).
date_created: 2018-12-11T11:46:55Z
date_published: 2013-09-10T00:00:00Z
date_updated: 2021-01-12T08:01:17Z
day: '10'
ddc:
- '581'
department:
- _id: JiFr
doi: 10.1038/msb.2013.40
file:
- access_level: open_access
checksum: 9c4fbe793af4bb22b3fe50cc677a39bf
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:07:46Z
date_updated: 2020-07-14T12:46:36Z
file_id: '4644'
file_name: IST-2018-936-v1+1_2008_Barton_A_map.pdf
file_size: 3257692
relation: main_file
file_date_updated: 2020-07-14T12:46:36Z
has_accepted_license: '1'
intvolume: ' 9'
issue: '1'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-sa/4.0/
month: '09'
oa: 1
oa_version: Published Version
publication: Molecular Systems Biology
publication_status: published
publisher: Nature Publishing Group
publist_id: '7303'
pubrep_id: '936'
quality_controlled: '1'
scopus_import: 1
status: public
title: A map of cell type‐specific auxin responses
tmp:
image: /images/cc_by_nc_sa.png
legal_code_url: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC
BY-NC-SA 4.0)
short: CC BY-NC-SA (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 9
year: '2013'
...
---
_id: '527'
abstract:
- lang: eng
text: The apical-basal axis of the early plant embryo determines the body plan of
the adult organism. To establish a polarized embryonic axis, plants evolved a
unique mechanism that involves directional, cell-to-cell transport of the growth
regulator auxin. Auxin transport relies on PIN auxin transporters [1], whose polar
subcellular localization determines the flow directionality. PIN-mediated auxin
transport mediates the spatial and temporal activity of the auxin response machinery
[2-7] that contributes to embryo patterning processes, including establishment
of the apical (shoot) and basal (root) embryo poles [8]. However, little is known
of upstream mechanisms guiding the (re)polarization of auxin fluxes during embryogenesis
[9]. Here, we developed a model of plant embryogenesis that correctly generates
emergent cell polarities and auxin-mediated sequential initiation of apical-basal
axis of plant embryo. The model relies on two precisely localized auxin sources
and a feedback between auxin and the polar, subcellular PIN transporter localization.
Simulations reproduced PIN polarity and auxin distribution, as well as previously
unknown polarization events during early embryogenesis. The spectrum of validated
model predictions suggests that our model corresponds to a minimal mechanistic
framework for initiation and orientation of the apical-basal axis to guide both
embryonic and postembryonic plant development.
author:
- first_name: Krzysztof T
full_name: Wabnik, Krzysztof T
id: 4DE369A4-F248-11E8-B48F-1D18A9856A87
last_name: Wabnik
orcid: 0000-0001-7263-0560
- first_name: Hélène
full_name: Robert, Hélène
last_name: Robert
- first_name: Richard
full_name: Smith, Richard
last_name: Smith
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Wabnik KT, Robert H, Smith R, Friml J. Modeling framework for the establishment
of the apical-basal embryonic axis in plants. Current Biology. 2013;23(24):2513-2518.
doi:10.1016/j.cub.2013.10.038
apa: Wabnik, K. T., Robert, H., Smith, R., & Friml, J. (2013). Modeling framework
for the establishment of the apical-basal embryonic axis in plants. Current
Biology. Cell Press. https://doi.org/10.1016/j.cub.2013.10.038
chicago: Wabnik, Krzysztof T, Hélène Robert, Richard Smith, and Jiří Friml. “Modeling
Framework for the Establishment of the Apical-Basal Embryonic Axis in Plants.”
Current Biology. Cell Press, 2013. https://doi.org/10.1016/j.cub.2013.10.038.
ieee: K. T. Wabnik, H. Robert, R. Smith, and J. Friml, “Modeling framework for the
establishment of the apical-basal embryonic axis in plants,” Current Biology,
vol. 23, no. 24. Cell Press, pp. 2513–2518, 2013.
ista: Wabnik KT, Robert H, Smith R, Friml J. 2013. Modeling framework for the establishment
of the apical-basal embryonic axis in plants. Current Biology. 23(24), 2513–2518.
mla: Wabnik, Krzysztof T., et al. “Modeling Framework for the Establishment of the
Apical-Basal Embryonic Axis in Plants.” Current Biology, vol. 23, no. 24,
Cell Press, 2013, pp. 2513–18, doi:10.1016/j.cub.2013.10.038.
short: K.T. Wabnik, H. Robert, R. Smith, J. Friml, Current Biology 23 (2013) 2513–2518.
date_created: 2018-12-11T11:46:58Z
date_published: 2013-12-16T00:00:00Z
date_updated: 2021-01-12T08:01:24Z
day: '16'
department:
- _id: EvBe
- _id: JiFr
doi: 10.1016/j.cub.2013.10.038
ec_funded: 1
intvolume: ' 23'
issue: '24'
language:
- iso: eng
month: '12'
oa_version: None
page: 2513 - 2518
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: Current Biology
publication_status: published
publisher: Cell Press
publist_id: '7292'
quality_controlled: '1'
scopus_import: 1
status: public
title: Modeling framework for the establishment of the apical-basal embryonic axis
in plants
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 23
year: '2013'
...
---
_id: '528'
abstract:
- lang: eng
text: Establishment of the embryonic axis foreshadows the main body axis of adults
both in plants and in animals, but underlying mechanisms are considered distinct.
Plants utilize directional, cell-to-cell transport of the growth hormone auxin
[1, 2] to generate an asymmetric auxin response that specifies the embryonic apical-basal
axis [3-6]. The auxin flow directionality depends on the polarized subcellular
localization of PIN-FORMED (PIN) auxin transporters [7, 8]. It remains unknown
which mechanisms and spatial cues guide cell polarization and axis orientation
in early embryos. Herein, we provide conceptually novel insights into the formation
of embryonic axis in Arabidopsis by identifying a crucial role of localized tryptophan-dependent
auxin biosynthesis [9-12]. Local auxin production at the base of young embryos
and the accompanying PIN7-mediated auxin flow toward the proembryo are required
for the apical auxin response maximum and the specification of apical embryonic
structures. Later in embryogenesis, the precisely timed onset of localized apical
auxin biosynthesis mediates PIN1 polarization, basal auxin response maximum, and
specification of the root pole. Thus, the tight spatiotemporal control of distinct
local auxin sources provides a necessary, non-cell-autonomous trigger for the
coordinated cell polarization and subsequent apical-basal axis orientation during
embryogenesis and, presumably, also for other polarization events during postembryonic
plant life [13, 14].
author:
- first_name: Hélène
full_name: Robert, Hélène
last_name: Robert
- first_name: Peter
full_name: Grones, Peter
id: 399876EC-F248-11E8-B48F-1D18A9856A87
last_name: Grones
- first_name: Anna
full_name: Stepanova, Anna
last_name: Stepanova
- first_name: Linda
full_name: Robles, Linda
last_name: Robles
- first_name: Annemarie
full_name: Lokerse, Annemarie
last_name: Lokerse
- first_name: Jose
full_name: Alonso, Jose
last_name: Alonso
- first_name: Dolf
full_name: Weijers, Dolf
last_name: Weijers
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Robert H, Grones P, Stepanova A, et al. Local auxin sources orient the apical
basal axis in arabidopsis embryos. Current Biology. 2013;23(24):2506-2512.
doi:10.1016/j.cub.2013.09.039
apa: Robert, H., Grones, P., Stepanova, A., Robles, L., Lokerse, A., Alonso, J.,
… Friml, J. (2013). Local auxin sources orient the apical basal axis in arabidopsis
embryos. Current Biology. Cell Press. https://doi.org/10.1016/j.cub.2013.09.039
chicago: Robert, Hélène, Peter Grones, Anna Stepanova, Linda Robles, Annemarie Lokerse,
Jose Alonso, Dolf Weijers, and Jiří Friml. “Local Auxin Sources Orient the Apical
Basal Axis in Arabidopsis Embryos.” Current Biology. Cell Press, 2013.
https://doi.org/10.1016/j.cub.2013.09.039.
ieee: H. Robert et al., “Local auxin sources orient the apical basal axis
in arabidopsis embryos,” Current Biology, vol. 23, no. 24. Cell Press,
pp. 2506–2512, 2013.
ista: Robert H, Grones P, Stepanova A, Robles L, Lokerse A, Alonso J, Weijers D,
Friml J. 2013. Local auxin sources orient the apical basal axis in arabidopsis
embryos. Current Biology. 23(24), 2506–2512.
mla: Robert, Hélène, et al. “Local Auxin Sources Orient the Apical Basal Axis in
Arabidopsis Embryos.” Current Biology, vol. 23, no. 24, Cell Press, 2013,
pp. 2506–12, doi:10.1016/j.cub.2013.09.039.
short: H. Robert, P. Grones, A. Stepanova, L. Robles, A. Lokerse, J. Alonso, D.
Weijers, J. Friml, Current Biology 23 (2013) 2506–2512.
date_created: 2018-12-11T11:46:59Z
date_published: 2013-12-16T00:00:00Z
date_updated: 2021-01-12T08:01:25Z
day: '16'
department:
- _id: JiFr
doi: 10.1016/j.cub.2013.09.039
ec_funded: 1
intvolume: ' 23'
issue: '24'
language:
- iso: eng
month: '12'
oa_version: None
page: 2506 - 2512
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: Current Biology
publication_status: published
publisher: Cell Press
publist_id: '7291'
quality_controlled: '1'
scopus_import: 1
status: public
title: Local auxin sources orient the apical basal axis in arabidopsis embryos
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 23
year: '2013'
...