---
_id: '1274'
abstract:
- lang: eng
text: Synchronized tissue polarization during regeneration or de novo vascular tissue
formation is a plant-specific example of intercellular communication and coordinated
development. According to the canalization hypothesis, the plant hormone auxin
serves as polarizing signal that mediates directional channel formation underlying
the spatio-temporal vasculature patterning. A necessary part of canalization is
a positive feedback between auxin signaling and polarity of the intercellular
auxin flow. The cellular and molecular mechanisms of this process are still poorly
understood, not the least, because of a lack of a suitable model system. We show
that the main genetic model plant, Arabidopsis (Arabidopsis thaliana) can be used
to study the canalization during vascular cambium regeneration and new vasculature
formation. We monitored localized auxin responses, directional auxin-transport
channels formation, and establishment of new vascular cambium polarity during
regenerative processes after stem wounding. The increased auxin response above
and around the wound preceded the formation of PIN1 auxin transporter-marked channels
from the primarily homogenous tissue and the transient, gradual changes in PIN1
localization preceded the polarity of newly formed vascular tissue. Thus, Arabidopsis
is a useful model for studies of coordinated tissue polarization and vasculature
formation after wounding allowing for genetic and mechanistic dissection of the
canalization hypothesis.
acknowledgement: We wish to thank Prof. Ewa U. Kurczyńska for initiation of this work
and valuable advices. We thank Martine De Cock for help in preparing the manuscript.
This work was supported by the European Research Council (project ERC-2011-StG-20101109-PSDP),
the European Social Fund (CZ.1.07/2.3.00/20.0043), and the Czech Science Foundation
GAČR (GA13-40637 S) to J.F., (GA 13-39982S) to E.B. and E.M. and in part by the
European Regional Development Fund (project “CEITEC, Central European Institute
of Technology”, CZ.1.05/1.1.00/02.0068).
article_number: '33754'
article_processing_charge: No
author:
- first_name: Ewa
full_name: Mazur, Ewa
last_name: Mazur
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Mazur E, Benková E, Friml J. Vascular cambium regeneration and vessel formation
in wounded inflorescence stems of Arabidopsis. Scientific Reports. 2016;6.
doi:10.1038/srep33754
apa: Mazur, E., Benková, E., & Friml, J. (2016). Vascular cambium regeneration
and vessel formation in wounded inflorescence stems of Arabidopsis. Scientific
Reports. Nature Publishing Group. https://doi.org/10.1038/srep33754
chicago: Mazur, Ewa, Eva Benková, and Jiří Friml. “Vascular Cambium Regeneration
and Vessel Formation in Wounded Inflorescence Stems of Arabidopsis.” Scientific
Reports. Nature Publishing Group, 2016. https://doi.org/10.1038/srep33754.
ieee: E. Mazur, E. Benková, and J. Friml, “Vascular cambium regeneration and vessel
formation in wounded inflorescence stems of Arabidopsis,” Scientific Reports,
vol. 6. Nature Publishing Group, 2016.
ista: Mazur E, Benková E, Friml J. 2016. Vascular cambium regeneration and vessel
formation in wounded inflorescence stems of Arabidopsis. Scientific Reports. 6,
33754.
mla: Mazur, Ewa, et al. “Vascular Cambium Regeneration and Vessel Formation in Wounded
Inflorescence Stems of Arabidopsis.” Scientific Reports, vol. 6, 33754,
Nature Publishing Group, 2016, doi:10.1038/srep33754.
short: E. Mazur, E. Benková, J. Friml, Scientific Reports 6 (2016).
date_created: 2018-12-11T11:51:05Z
date_published: 2016-09-21T00:00:00Z
date_updated: 2025-05-07T11:12:28Z
day: '21'
ddc:
- '581'
department:
- _id: EvBe
- _id: JiFr
doi: 10.1038/srep33754
external_id:
pmid:
- '27649687'
file:
- access_level: open_access
checksum: ee371fbc9124ad93157a95829264e4fe
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:13:25Z
date_updated: 2020-07-14T12:44:42Z
file_id: '5008'
file_name: IST-2016-692-v1+1_srep33754.pdf
file_size: 2895147
relation: main_file
file_date_updated: 2020-07-14T12:44:42Z
has_accepted_license: '1'
intvolume: ' 6'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '09'
oa: 1
oa_version: Published Version
pmid: 1
publication: Scientific Reports
publication_status: published
publisher: Nature Publishing Group
publist_id: '6042'
pubrep_id: '692'
quality_controlled: '1'
related_material:
record:
- id: '545'
relation: later_version
status: public
scopus_import: '1'
status: public
title: Vascular cambium regeneration and vessel formation in wounded inflorescence
stems of Arabidopsis
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 6
year: '2016'
...
---
_id: '1277'
abstract:
- lang: eng
text: "The Arabidopsis thaliana endogenous elicitor peptides (AtPeps) are released
into the apoplast after cellular damage caused by pathogens or wounding to induce
innate immunity by direct binding to the membrane-localized leucine-rich repeat
receptor kinases, PEP RECEPTOR1 (PEPR1) and PEPR2. Although the PEPR-mediated
signaling components and responses have been studied extensively, the contributions
of the subcellular localization and dynamics of the active PEPRs remain largely
unknown. We used live-cell imaging of the fluorescently labeled and bioactive
pep1 to visualize the intracellular behavior of the PEPRs in the Arabidopsis root
meristem. We found that AtPep1 decorated the plasma membrane (PM) in a receptor-dependent
manner and cointernalized with PEPRs. Trafficking of the AtPep1-PEPR1 complexes
to the vacuole required neither the trans-Golgi network/early endosome (TGN/EE)-localized
vacuolar H+ -ATPase activity nor the function of the brefeldin A-sensitive ADP-ribosylation
factor-guanine exchange factors (ARF-GEFs). In addition, AtPep1 and different
TGN/EE markers colocalized only rarely, implying that the intracellular route
of this receptor-ligand pair is largely independent of the TGN/EE. Inducible overexpression
of the Arabidopsis clathrin coat disassembly factor, Auxilin2, which inhibits
clathrin-mediated endocytosis (CME), impaired the AtPep1-PEPR1 internalization
and compromised AtPep1-mediated responses. Our results show that clathrin function
at the PM is required to induce plant defense responses, likely through CME of
cell surface-located signaling components.\r\n"
acknowledgement: "F.A.O.-M. was supported by special\r\nresearch funding from the
Flemish Government for a joint doctorate fellowship\r\nat Ghent University, and
funding from the Student Program\r\n–\r\nGraduate Studies\r\nPlan Program from the
Coordination for the Improvement of Higher Educa-\r\ntion Personnel, Brazil, for
a doctorate fellowship at the University of São Paulo.\r\nX.Z. and Q.L. are indebted
to the China Science Council and G.P.d.O. to the\r\n“\r\nCiência sem Fronteiras\r\n”\r\nfor
predoctoral fellowships. R.K. and Y.L. have re-\r\nceived postdoctoral fellowships
from the Belgian Science Policy Office. This\r\nresearch was supported by Flanders
Research Foundation Grant G008416N\r\n(to E.R.) and by the São Paulo Research Foundation
and the National Council\r\nfor Scientific and Technological Development (CNPq)
(D.S.d.M.). D.S.d.M. is a\r\nresearch fellow of CNPq.\r\nWe thank D. Van Damme,
E. Mylle, M. Castro Silva-Filho,\r\nand J. Goeman for providing usefu\r\nl advice
and technical assistance;\r\nI. Hara-Nishimura, J. Lin, G. Jürgens, M. A. Johnson,
and P. Bozhkov for sharing\r\npublished materials; and M. Nowack and M. Fendrych
for kindly donating the\r\npUBQ10::ATG8-YFP\r\n-expressing marker line."
author:
- first_name: Fausto
full_name: Ortiz Morea, Fausto
last_name: Ortiz Morea
- first_name: Daniel
full_name: Savatin, Daniel
last_name: Savatin
- first_name: Wim
full_name: Dejonghe, Wim
last_name: Dejonghe
- first_name: Rahul
full_name: Kumar, Rahul
last_name: Kumar
- first_name: Yu
full_name: Luo, Yu
last_name: Luo
- first_name: Maciek
full_name: Adamowski, Maciek
id: 45F536D2-F248-11E8-B48F-1D18A9856A87
last_name: Adamowski
orcid: 0000-0001-6463-5257
- first_name: Jos
full_name: Van Begin, Jos
last_name: Van Begin
- first_name: Keini
full_name: Dressano, Keini
last_name: Dressano
- first_name: Guilherme
full_name: De Oliveira, Guilherme
last_name: De Oliveira
- first_name: Xiuyang
full_name: Zhao, Xiuyang
last_name: Zhao
- first_name: Qing
full_name: Lu, Qing
last_name: Lu
- first_name: Annemieke
full_name: Madder, Annemieke
last_name: Madder
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Daniel
full_name: De Moura, Daniel
last_name: De Moura
- first_name: Eugenia
full_name: Russinova, Eugenia
last_name: Russinova
citation:
ama: Ortiz Morea F, Savatin D, Dejonghe W, et al. Danger-associated peptide signaling
in Arabidopsis requires clathrin. PNAS. 2016;113(39):11028-11033. doi:10.1073/pnas.1605588113
apa: Ortiz Morea, F., Savatin, D., Dejonghe, W., Kumar, R., Luo, Y., Adamowski,
M., … Russinova, E. (2016). Danger-associated peptide signaling in Arabidopsis
requires clathrin. PNAS. National Academy of Sciences. https://doi.org/10.1073/pnas.1605588113
chicago: Ortiz Morea, Fausto, Daniel Savatin, Wim Dejonghe, Rahul Kumar, Yu Luo,
Maciek Adamowski, Jos Van Begin, et al. “Danger-Associated Peptide Signaling in
Arabidopsis Requires Clathrin.” PNAS. National Academy of Sciences, 2016.
https://doi.org/10.1073/pnas.1605588113.
ieee: F. Ortiz Morea et al., “Danger-associated peptide signaling in Arabidopsis
requires clathrin,” PNAS, vol. 113, no. 39. National Academy of Sciences,
pp. 11028–11033, 2016.
ista: Ortiz Morea F, Savatin D, Dejonghe W, Kumar R, Luo Y, Adamowski M, Van Begin
J, Dressano K, De Oliveira G, Zhao X, Lu Q, Madder A, Friml J, De Moura D, Russinova
E. 2016. Danger-associated peptide signaling in Arabidopsis requires clathrin.
PNAS. 113(39), 11028–11033.
mla: Ortiz Morea, Fausto, et al. “Danger-Associated Peptide Signaling in Arabidopsis
Requires Clathrin.” PNAS, vol. 113, no. 39, National Academy of Sciences,
2016, pp. 11028–33, doi:10.1073/pnas.1605588113.
short: F. Ortiz Morea, D. Savatin, W. Dejonghe, R. Kumar, Y. Luo, M. Adamowski,
J. Van Begin, K. Dressano, G. De Oliveira, X. Zhao, Q. Lu, A. Madder, J. Friml,
D. De Moura, E. Russinova, PNAS 113 (2016) 11028–11033.
date_created: 2018-12-11T11:51:06Z
date_published: 2016-09-27T00:00:00Z
date_updated: 2021-01-12T06:49:34Z
day: '27'
department:
- _id: JiFr
doi: 10.1073/pnas.1605588113
intvolume: ' 113'
issue: '39'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5047203/
month: '09'
oa: 1
oa_version: Preprint
page: 11028 - 11033
publication: PNAS
publication_status: published
publisher: National Academy of Sciences
publist_id: '6039'
quality_controlled: '1'
scopus_import: 1
status: public
title: Danger-associated peptide signaling in Arabidopsis requires clathrin
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 113
year: '2016'
...
---
_id: '1640'
abstract:
- lang: eng
text: Auxin and cytokinin are key endogenous regulators of plant development. Although
cytokinin-mediated modulation of auxin distribution is a developmentally crucial
hormonal interaction, its molecular basis is largely unknown. Here we show a direct
regulatory link between cytokinin signalling and the auxin transport machinery
uncovering a mechanistic framework for cytokinin-auxin cross-talk. We show that
the CYTOKININ RESPONSE FACTORS (CRFs), transcription factors downstream of cytokinin
perception, transcriptionally control genes encoding PIN-FORMED (PIN) auxin transporters
at a specific PIN CYTOKININ RESPONSE ELEMENT (PCRE) domain. Removal of this cis-regulatory
element effectively uncouples PIN transcription from the CRF-mediated cytokinin
regulation and attenuates plant cytokinin sensitivity. We propose that CRFs represent
a missing cross-talk component that fine-tunes auxin transport capacity downstream
of cytokinin signalling to control plant development.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: This work was supported by the European Research Council Starting
Independent Research grant (ERC-2007-Stg-207362-HCPO to E.B., M.S., C.C.), by the
Ghent University Multidisciplinary Research Partnership ‘Biotechnology for a Sustainable
Economy’ no.01MRB510W, by the Research Foundation—Flanders (grant 3G033711 to J.-A.O.),
by the Austrian Science Fund (FWF01_I1774S) to K.Ö.,E.B., and by the Interuniversity
Attraction Poles Programme (IUAP P7/29 ‘MARS’) initiated by the Belgian Science
Policy Office. I.D.C. and S.V. are post-doctoral fellows of the Research Foundation—Flanders
(FWO). This research was supported by the Scientific Service Units (SSU) of IST-Austria
through resources provided by the Bioimaging Facility (BIF), the Life Science Facility
(LSF).
article_number: '8717'
author:
- first_name: Mária
full_name: Šimášková, Mária
last_name: Šimášková
- first_name: José
full_name: O'Brien, José
last_name: O'Brien
- first_name: Mamoona
full_name: Khan-Djamei, Mamoona
id: 391B5BBC-F248-11E8-B48F-1D18A9856A87
last_name: Khan-Djamei
- first_name: Giel
full_name: Van Noorden, Giel
last_name: Van Noorden
- first_name: Krisztina
full_name: Ötvös, Krisztina
id: 29B901B0-F248-11E8-B48F-1D18A9856A87
last_name: Ötvös
orcid: 0000-0002-5503-4983
- first_name: Anne
full_name: Vieten, Anne
last_name: Vieten
- first_name: Inge
full_name: De Clercq, Inge
last_name: De Clercq
- first_name: Johanna
full_name: Van Haperen, Johanna
last_name: Van Haperen
- first_name: Candela
full_name: Cuesta, Candela
id: 33A3C818-F248-11E8-B48F-1D18A9856A87
last_name: Cuesta
orcid: 0000-0003-1923-2410
- first_name: Klára
full_name: Hoyerová, Klára
last_name: Hoyerová
- first_name: Steffen
full_name: Vanneste, Steffen
last_name: Vanneste
- first_name: Peter
full_name: Marhavy, Peter
id: 3F45B078-F248-11E8-B48F-1D18A9856A87
last_name: Marhavy
orcid: 0000-0001-5227-5741
- first_name: Krzysztof T
full_name: Wabnik, Krzysztof T
id: 4DE369A4-F248-11E8-B48F-1D18A9856A87
last_name: Wabnik
orcid: 0000-0001-7263-0560
- first_name: Frank
full_name: Van Breusegem, Frank
last_name: Van Breusegem
- first_name: Moritz
full_name: Nowack, Moritz
last_name: Nowack
- first_name: Angus
full_name: Murphy, Angus
last_name: Murphy
- first_name: Jiřĺ
full_name: Friml, Jiřĺ
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Dolf
full_name: Weijers, Dolf
last_name: Weijers
- first_name: Tom
full_name: Beeckman, Tom
last_name: Beeckman
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
citation:
ama: Šimášková M, O’Brien J, Khan-Djamei M, et al. Cytokinin response factors regulate
PIN-FORMED auxin transporters. Nature Communications. 2015;6. doi:10.1038/ncomms9717
apa: Šimášková, M., O’Brien, J., Khan-Djamei, M., Van Noorden, G., Ötvös, K., Vieten,
A., … Benková, E. (2015). Cytokinin response factors regulate PIN-FORMED auxin
transporters. Nature Communications. Nature Publishing Group. https://doi.org/10.1038/ncomms9717
chicago: Šimášková, Mária, José O’Brien, Mamoona Khan-Djamei, Giel Van Noorden,
Krisztina Ötvös, Anne Vieten, Inge De Clercq, et al. “Cytokinin Response Factors
Regulate PIN-FORMED Auxin Transporters.” Nature Communications. Nature
Publishing Group, 2015. https://doi.org/10.1038/ncomms9717.
ieee: M. Šimášková et al., “Cytokinin response factors regulate PIN-FORMED
auxin transporters,” Nature Communications, vol. 6. Nature Publishing Group,
2015.
ista: Šimášková M, O’Brien J, Khan-Djamei M, Van Noorden G, Ötvös K, Vieten A, De
Clercq I, Van Haperen J, Cuesta C, Hoyerová K, Vanneste S, Marhavý P, Wabnik KT,
Van Breusegem F, Nowack M, Murphy A, Friml J, Weijers D, Beeckman T, Benková E.
2015. Cytokinin response factors regulate PIN-FORMED auxin transporters. Nature
Communications. 6, 8717.
mla: Šimášková, Mária, et al. “Cytokinin Response Factors Regulate PIN-FORMED Auxin
Transporters.” Nature Communications, vol. 6, 8717, Nature Publishing Group,
2015, doi:10.1038/ncomms9717.
short: M. Šimášková, J. O’Brien, M. Khan-Djamei, G. Van Noorden, K. Ötvös, A. Vieten,
I. De Clercq, J. Van Haperen, C. Cuesta, K. Hoyerová, S. Vanneste, P. Marhavý,
K.T. Wabnik, F. Van Breusegem, M. Nowack, A. Murphy, J. Friml, D. Weijers, T.
Beeckman, E. Benková, Nature Communications 6 (2015).
date_created: 2018-12-11T11:53:12Z
date_published: 2015-01-01T00:00:00Z
date_updated: 2021-01-12T06:52:11Z
day: '01'
ddc:
- '580'
department:
- _id: EvBe
- _id: JiFr
doi: 10.1038/ncomms9717
ec_funded: 1
file:
- access_level: open_access
checksum: c2c84bca37401435fedf76bad0ba0579
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:18:36Z
date_updated: 2020-07-14T12:45:08Z
file_id: '5358'
file_name: IST-2018-1020-v1+1_Simaskova_et_al_NatCom_2015.pdf
file_size: 1471217
relation: main_file
file_date_updated: 2020-07-14T12:45:08Z
has_accepted_license: '1'
intvolume: ' 6'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Submitted Version
project:
- _id: 253FCA6A-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '207362'
name: Hormonal cross-talk in plant organogenesis
- _id: 2542D156-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I 1774-B16
name: Hormone cross-talk drives nutrient dependent plant development
publication: Nature Communications
publication_status: published
publisher: Nature Publishing Group
publist_id: '5513'
pubrep_id: '1020'
quality_controlled: '1'
scopus_import: 1
status: public
title: Cytokinin response factors regulate PIN-FORMED auxin transporters
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 6
year: '2015'
...
---
_id: '1819'
abstract:
- lang: eng
text: 'The sessile life style of plants creates the need to deal with an often adverse
environment, in which water availability can change on a daily basis, challenging
the cellular physiology and integrity. Changes in osmotic conditions disrupt the
equilibrium of the plasma membrane: hypoosmotic conditions increase and hyperosmotic
environment decrease the cell volume. Here, we show that short-term extracellular
osmotic treatments are closely followed by a shift in the balance between endocytosis
and exocytosis in root meristem cells. Acute hyperosmotic treatments (ionic and
nonionic) enhance clathrin-mediated endocytosis simultaneously attenuating exocytosis,
whereas hypoosmotic treatments have the opposite effects. In addition to clathrin
recruitment to the plasma membrane, components of early endocytic trafficking
are essential during hyperosmotic stress responses. Consequently, growth of seedlings
defective in elements of clathrin or early endocytic machinery is more sensitive
to hyperosmotic treatments. We also found that the endocytotic response to a change
of osmotic status in the environment is dominant over the presumably evolutionary
more recent regulatory effect of plant hormones, such as auxin. These results
imply that osmotic perturbation influences the balance between endocytosis and
exocytosis acting through clathrin-mediated endocytosis. We propose that tension
on the plasma membrane determines the addition or removal of membranes at the
cell surface, thus preserving cell integrity.'
acknowledgement: This work was supported by the European Research Council (project
ERC-2011-StG-20101109-PSDP); European Social Fund (CZ.1.07/2.3.00/20.0043) and the
Czech Science Foundation GAČR (GA13-40637S) to J.F.; project Postdoc I. (CZ.1.07/2.3.00/30.0009)
co-financed by the European Social Fund and the state budget of the Czech Republic
to M.Z. and T.N..
author:
- first_name: Marta
full_name: Zwiewka, Marta
last_name: Zwiewka
- first_name: Tomasz
full_name: Nodzyński, Tomasz
last_name: Nodzyński
- first_name: Stéphanie
full_name: Robert, Stéphanie
last_name: Robert
- first_name: Steffen
full_name: Vanneste, Steffen
last_name: Vanneste
- first_name: Jiřĺ
full_name: Friml, Jiřĺ
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Zwiewka M, Nodzyński T, Robert S, Vanneste S, Friml J. Osmotic stress modulates
the balance between exocytosis and clathrin mediated endocytosis in Arabidopsis
thaliana. Molecular Plant. 2015;8(8):1175-1187. doi:10.1016/j.molp.2015.03.007
apa: Zwiewka, M., Nodzyński, T., Robert, S., Vanneste, S., & Friml, J. (2015).
Osmotic stress modulates the balance between exocytosis and clathrin mediated
endocytosis in Arabidopsis thaliana. Molecular Plant. Elsevier. https://doi.org/10.1016/j.molp.2015.03.007
chicago: Zwiewka, Marta, Tomasz Nodzyński, Stéphanie Robert, Steffen Vanneste, and
Jiří Friml. “Osmotic Stress Modulates the Balance between Exocytosis and Clathrin
Mediated Endocytosis in Arabidopsis Thaliana.” Molecular Plant. Elsevier,
2015. https://doi.org/10.1016/j.molp.2015.03.007.
ieee: M. Zwiewka, T. Nodzyński, S. Robert, S. Vanneste, and J. Friml, “Osmotic stress
modulates the balance between exocytosis and clathrin mediated endocytosis in
Arabidopsis thaliana,” Molecular Plant, vol. 8, no. 8. Elsevier, pp. 1175–1187,
2015.
ista: Zwiewka M, Nodzyński T, Robert S, Vanneste S, Friml J. 2015. Osmotic stress
modulates the balance between exocytosis and clathrin mediated endocytosis in
Arabidopsis thaliana. Molecular Plant. 8(8), 1175–1187.
mla: Zwiewka, Marta, et al. “Osmotic Stress Modulates the Balance between Exocytosis
and Clathrin Mediated Endocytosis in Arabidopsis Thaliana.” Molecular Plant,
vol. 8, no. 8, Elsevier, 2015, pp. 1175–87, doi:10.1016/j.molp.2015.03.007.
short: M. Zwiewka, T. Nodzyński, S. Robert, S. Vanneste, J. Friml, Molecular Plant
8 (2015) 1175–1187.
date_created: 2018-12-11T11:54:11Z
date_published: 2015-08-03T00:00:00Z
date_updated: 2021-01-12T06:53:24Z
day: '03'
department:
- _id: JiFr
doi: 10.1016/j.molp.2015.03.007
ec_funded: 1
intvolume: ' 8'
issue: '8'
language:
- iso: eng
month: '08'
oa_version: None
page: 1175 - 1187
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: Molecular Plant
publication_status: published
publisher: Elsevier
publist_id: '5287'
quality_controlled: '1'
scopus_import: 1
status: public
title: Osmotic stress modulates the balance between exocytosis and clathrin mediated
endocytosis in Arabidopsis thaliana
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 8
year: '2015'
...
---
_id: '1847'
acknowledgement: This work was supported by the European Research Council (project
ERC-2011-StG-20101109-PSDP), European Social Fund (CZ.1.07/2.3.00/20.0043), and
the Czech Science Foundation GAČR (GA13-40637S).
author:
- first_name: Peter
full_name: Grones, Peter
id: 399876EC-F248-11E8-B48F-1D18A9856A87
last_name: Grones
- first_name: Jiřĺ
full_name: Friml, Jiřĺ
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: 'Grones P, Friml J. ABP1: Finally docking. Molecular Plant. 2015;8(3):356-358.
doi:10.1016/j.molp.2014.12.013'
apa: 'Grones, P., & Friml, J. (2015). ABP1: Finally docking. Molecular Plant.
Elsevier. https://doi.org/10.1016/j.molp.2014.12.013'
chicago: 'Grones, Peter, and Jiří Friml. “ABP1: Finally Docking.” Molecular Plant.
Elsevier, 2015. https://doi.org/10.1016/j.molp.2014.12.013.'
ieee: 'P. Grones and J. Friml, “ABP1: Finally docking,” Molecular Plant,
vol. 8, no. 3. Elsevier, pp. 356–358, 2015.'
ista: 'Grones P, Friml J. 2015. ABP1: Finally docking. Molecular Plant. 8(3), 356–358.'
mla: 'Grones, Peter, and Jiří Friml. “ABP1: Finally Docking.” Molecular Plant,
vol. 8, no. 3, Elsevier, 2015, pp. 356–58, doi:10.1016/j.molp.2014.12.013.'
short: P. Grones, J. Friml, Molecular Plant 8 (2015) 356–358.
date_created: 2018-12-11T11:54:20Z
date_published: 2015-03-02T00:00:00Z
date_updated: 2021-01-12T06:53:35Z
day: '02'
department:
- _id: JiFr
doi: 10.1016/j.molp.2014.12.013
intvolume: ' 8'
issue: '3'
language:
- iso: eng
month: '03'
oa_version: None
page: 356 - 358
publication: Molecular Plant
publication_status: published
publisher: Elsevier
publist_id: '5254'
quality_controlled: '1'
scopus_import: 1
status: public
title: 'ABP1: Finally docking'
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 8
year: '2015'
...
---
_id: '1849'
abstract:
- lang: eng
text: 'Cell polarity is a fundamental property of pro- and eukaryotic cells. It
is necessary for coordination of cell division, cell morphogenesis and signaling
processes. How polarity is generated and maintained is a complex issue governed
by interconnected feed-back regulations between small GTPase signaling and membrane
tension-based signaling that controls membrane trafficking, and cytoskeleton organization
and dynamics. Here, we will review the potential role for calcium as a crucial
signal that connects and coordinates the respective processes during polarization
processes in plants. This article is part of a Special Issue entitled: 13th European
Symposium on Calcium.'
acknowledgement: The contributing authors were supported by the Ghent University Special
Research Fund (to E.H.), the Interuniversity Attraction Poles Programme (IAP VI/33
and IUAP P7/29 ‘MARS’), the European Research Council (project ERC-2011-StG-20101109-PSDP,
to J.F.), and the Research Foundation Flanders (to S.V.).
author:
- first_name: Ellie
full_name: Himschoot, Ellie
last_name: Himschoot
- first_name: Tom
full_name: Beeckman, Tom
last_name: Beeckman
- first_name: Jiřĺ
full_name: Friml, Jiřĺ
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Steffen
full_name: Vanneste, Steffen
last_name: Vanneste
citation:
ama: Himschoot E, Beeckman T, Friml J, Vanneste S. Calcium is an organizer of cell
polarity in plants. Biochimica et Biophysica Acta - Molecular Cell Research.
2015;1853(9):2168-2172. doi:10.1016/j.bbamcr.2015.02.017
apa: Himschoot, E., Beeckman, T., Friml, J., & Vanneste, S. (2015). Calcium
is an organizer of cell polarity in plants. Biochimica et Biophysica Acta -
Molecular Cell Research. Elsevier. https://doi.org/10.1016/j.bbamcr.2015.02.017
chicago: Himschoot, Ellie, Tom Beeckman, Jiří Friml, and Steffen Vanneste. “Calcium
Is an Organizer of Cell Polarity in Plants.” Biochimica et Biophysica Acta
- Molecular Cell Research. Elsevier, 2015. https://doi.org/10.1016/j.bbamcr.2015.02.017.
ieee: E. Himschoot, T. Beeckman, J. Friml, and S. Vanneste, “Calcium is an organizer
of cell polarity in plants,” Biochimica et Biophysica Acta - Molecular Cell
Research, vol. 1853, no. 9. Elsevier, pp. 2168–2172, 2015.
ista: Himschoot E, Beeckman T, Friml J, Vanneste S. 2015. Calcium is an organizer
of cell polarity in plants. Biochimica et Biophysica Acta - Molecular Cell Research.
1853(9), 2168–2172.
mla: Himschoot, Ellie, et al. “Calcium Is an Organizer of Cell Polarity in Plants.”
Biochimica et Biophysica Acta - Molecular Cell Research, vol. 1853, no.
9, Elsevier, 2015, pp. 2168–72, doi:10.1016/j.bbamcr.2015.02.017.
short: E. Himschoot, T. Beeckman, J. Friml, S. Vanneste, Biochimica et Biophysica
Acta - Molecular Cell Research 1853 (2015) 2168–2172.
date_created: 2018-12-11T11:54:21Z
date_published: 2015-09-01T00:00:00Z
date_updated: 2021-01-12T06:53:36Z
day: '01'
department:
- _id: JiFr
doi: 10.1016/j.bbamcr.2015.02.017
intvolume: ' 1853'
issue: '9'
language:
- iso: eng
month: '09'
oa_version: None
page: 2168 - 2172
publication: Biochimica et Biophysica Acta - Molecular Cell Research
publication_status: published
publisher: Elsevier
publist_id: '5252'
quality_controlled: '1'
scopus_import: 1
status: public
title: Calcium is an organizer of cell polarity in plants
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 1853
year: '2015'
...
---
_id: '1865'
abstract:
- lang: eng
text: The plant hormone auxin and its directional transport are known to play a
crucial role in defining the embryonic axis and subsequent development of the
body plan. Although the role of PIN auxin efflux transporters has been clearly
assigned during embryonic shoot and root specification, the role of the auxin
influx carriers AUX1 and LIKE-AUX1 (LAX) proteins is not well established. Here,
we used chemical and genetic tools on Brassica napus microspore-derived embryos
and Arabidopsis thaliana zygotic embryos, and demonstrate that AUX1, LAX1 and
LAX2 are required for both shoot and root pole formation, in concert with PIN
efflux carriers. Furthermore, we uncovered a positive-feedback loop betweenMONOPTEROS(ARF5)-dependent
auxin signalling and auxin transport. ThisMONOPTEROSdependent transcriptional
regulation of auxin influx (AUX1, LAX1 and LAX2) and auxin efflux (PIN1 and PIN4)
carriers by MONOPTEROS helps to maintain proper auxin transport to the root tip.
These results indicate that auxin-dependent cell specification during embryo development
requires balanced auxin transport involving both influx and efflux mechanisms,
and that this transport is maintained by a positive transcriptional feedback on
auxin signalling.
acknowledgement: W.G. is a post-doctoral fellow of the Research Foundation Flanders.
H.S.R. is supported by Employment of Best Young Scientists for International Cooperation
Empowerment [CZ.1.07/2.3.00/30.0037], co-financed by the European Social Fund and
the state budget of the Czech Republic. Mi.S. was funded by the Ramón y Cajal program.
This work was supported by the European Research Council [project ERC-2011-StG-20101109-PSDP],
project ‘CEITEC – Central European Institute of Technology’ [CZ.1.05/1.1.00/02.0068],
the European Social Fund [CZ.1.07/2.3.00/20.0043] and the Czech Science Foundation
GACR [GA13-40637S] to J.F. We acknowledge funding from the Biological and Biotechnological
Science Research Council (BBSRC) and Engineering Physics Science Research Council
(EPSRC) to R.S. and M.B
author:
- first_name: Hélène
full_name: Robert, Hélène
last_name: Robert
- first_name: Wim
full_name: Grunewald, Wim
last_name: Grunewald
- first_name: Michael
full_name: Sauer, Michael
last_name: Sauer
- first_name: Bernard
full_name: Cannoot, Bernard
last_name: Cannoot
- first_name: Mercedes
full_name: Soriano, Mercedes
last_name: Soriano
- first_name: Ranjan
full_name: Swarup, Ranjan
last_name: Swarup
- first_name: Dolf
full_name: Weijers, Dolf
last_name: Weijers
- first_name: Malcolm
full_name: Bennett, Malcolm
last_name: Bennett
- first_name: Kim
full_name: Boutilier, Kim
last_name: Boutilier
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Robert H, Grunewald W, Sauer M, et al. Plant embryogenesis requires AUX/LAX-mediated
auxin influx. Development. 2015;142(4):702-711. doi:10.1242/dev.115832
apa: Robert, H., Grunewald, W., Sauer, M., Cannoot, B., Soriano, M., Swarup, R.,
… Friml, J. (2015). Plant embryogenesis requires AUX/LAX-mediated auxin influx.
Development. Company of Biologists. https://doi.org/10.1242/dev.115832
chicago: Robert, Hélène, Wim Grunewald, Michael Sauer, Bernard Cannoot, Mercedes
Soriano, Ranjan Swarup, Dolf Weijers, Malcolm Bennett, Kim Boutilier, and Jiří
Friml. “Plant Embryogenesis Requires AUX/LAX-Mediated Auxin Influx.” Development.
Company of Biologists, 2015. https://doi.org/10.1242/dev.115832.
ieee: H. Robert et al., “Plant embryogenesis requires AUX/LAX-mediated auxin
influx,” Development, vol. 142, no. 4. Company of Biologists, pp. 702–711,
2015.
ista: Robert H, Grunewald W, Sauer M, Cannoot B, Soriano M, Swarup R, Weijers D,
Bennett M, Boutilier K, Friml J. 2015. Plant embryogenesis requires AUX/LAX-mediated
auxin influx. Development. 142(4), 702–711.
mla: Robert, Hélène, et al. “Plant Embryogenesis Requires AUX/LAX-Mediated Auxin
Influx.” Development, vol. 142, no. 4, Company of Biologists, 2015, pp.
702–11, doi:10.1242/dev.115832.
short: H. Robert, W. Grunewald, M. Sauer, B. Cannoot, M. Soriano, R. Swarup, D.
Weijers, M. Bennett, K. Boutilier, J. Friml, Development 142 (2015) 702–711.
date_created: 2018-12-11T11:54:26Z
date_published: 2015-02-15T00:00:00Z
date_updated: 2021-01-12T06:53:43Z
day: '15'
department:
- _id: JiFr
doi: 10.1242/dev.115832
ec_funded: 1
intvolume: ' 142'
issue: '4'
language:
- iso: eng
month: '02'
oa_version: None
page: 702 - 711
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: Development
publication_status: published
publisher: Company of Biologists
publist_id: '5231'
quality_controlled: '1'
scopus_import: 1
status: public
title: Plant embryogenesis requires AUX/LAX-mediated auxin influx
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 142
year: '2015'
...
---
_id: '1871'
abstract:
- lang: eng
text: The plant hormone auxin is a key regulator of plant growth and development.
Differences in auxin distribution within tissues are mediated by the polar auxin
transport machinery, and cellular auxin responses occur depending on changes in
cellular auxin levels. Multiple receptor systems at the cell surface and in the
interior operate to sense and interpret fluctuations in auxin distribution that
occur during plant development. Until now, three proteins or protein complexes
that can bind auxin have been identified. SCFTIR1 [a SKP1-cullin-1-F-box complex
that contains transport inhibitor response 1 (TIR1) as the F-box protein] and
S-phase-kinaseassociated protein 2 (SKP2) localize to the nucleus, whereas auxinbinding
protein 1 (ABP1), predominantly associates with the endoplasmic reticulum and
cell surface. In this Cell Science at a Glance article, we summarize recent discoveries
in the field of auxin transport and signaling that have led to the identification
of new components of these pathways, as well as their mutual interaction.
acknowledgement: This work was supported by the European Research Council [project
ERC-2011-StG-20101109-PSDP]; European Social Fund [grant number CZ.1.07/2.3.00/20.0043]
and the Czech Science Foundation GAČR [grant number GA13-40637S]
author:
- first_name: Peter
full_name: Grones, Peter
id: 399876EC-F248-11E8-B48F-1D18A9856A87
last_name: Grones
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Grones P, Friml J. Auxin transporters and binding proteins at a glance. Journal
of Cell Science. 2015;128(1):1-7. doi:10.1242/jcs.159418
apa: Grones, P., & Friml, J. (2015). Auxin transporters and binding proteins
at a glance. Journal of Cell Science. Company of Biologists. https://doi.org/10.1242/jcs.159418
chicago: Grones, Peter, and Jiří Friml. “Auxin Transporters and Binding Proteins
at a Glance.” Journal of Cell Science. Company of Biologists, 2015. https://doi.org/10.1242/jcs.159418.
ieee: P. Grones and J. Friml, “Auxin transporters and binding proteins at a glance,”
Journal of Cell Science, vol. 128, no. 1. Company of Biologists, pp. 1–7,
2015.
ista: Grones P, Friml J. 2015. Auxin transporters and binding proteins at a glance.
Journal of Cell Science. 128(1), 1–7.
mla: Grones, Peter, and Jiří Friml. “Auxin Transporters and Binding Proteins at
a Glance.” Journal of Cell Science, vol. 128, no. 1, Company of Biologists,
2015, pp. 1–7, doi:10.1242/jcs.159418.
short: P. Grones, J. Friml, Journal of Cell Science 128 (2015) 1–7.
date_created: 2018-12-11T11:54:28Z
date_published: 2015-01-01T00:00:00Z
date_updated: 2021-01-12T06:53:45Z
day: '01'
ddc:
- '570'
department:
- _id: JiFr
doi: 10.1242/jcs.159418
file:
- access_level: open_access
checksum: 24c779f4cd9d549ca6833e26f486be27
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:11:00Z
date_updated: 2020-07-14T12:45:19Z
file_id: '4852'
file_name: IST-2016-563-v1+1_1.full.pdf
file_size: 1688844
relation: main_file
file_date_updated: 2020-07-14T12:45:19Z
has_accepted_license: '1'
intvolume: ' 128'
issue: '1'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Submitted Version
page: 1 - 7
publication: Journal of Cell Science
publication_status: published
publisher: Company of Biologists
publist_id: '5225'
pubrep_id: '563'
quality_controlled: '1'
scopus_import: 1
status: public
title: Auxin transporters and binding proteins at a glance
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 128
year: '2015'
...
---
_id: '1878'
abstract:
- lang: eng
text: Petrocoptis is a small genus of chasmophytic plants endemic to the Iberian
Peninsula, with some localized populations in the French Pyrenees. Within the
genus, a dozen species have been recognized based on morphological diversity,
most of them with limited distribution area, in small populations and frequently
with potential threats to their survival. To date, however, a molecular evaluation
of the current systematic treatments has not been carried out. The aim of the
present study is to infer phylogenetic relationships among its subordinate taxa
by using plastidial rps16 intron and nuclear internal transcribed spacer (ITS)
DNA sequences; and evaluate the phylogenetic placement of the genus Petrocoptis
within the family Caryophyllaceae. The monophyly of Petrocoptis is supported by
both ITS and rps16 intron sequence analyses. Furthermore, time estimates using
BEAST analyses indicate a Middle to Late Miocene diversification (10.59 Myr, 6.44–15.26
Myr highest posterior densities [HPD], for ITS; 14.30 Myr, 8.61–21.00 Myr HPD,
for rps16 intron).
author:
- first_name: Eduardo
full_name: Cires Rodriguez, Eduardo
id: 2AD56A7A-F248-11E8-B48F-1D18A9856A87
last_name: Cires Rodriguez
- first_name: José
full_name: Prieto, José
last_name: Prieto
citation:
ama: Cires Rodriguez E, Prieto J. Phylogenetic relationships of Petrocoptis A. Braun
ex Endl. (Caryophyllaceae), a discussed genus from the Iberian Peninsula. Journal
of Plant Research. 2015;128(2):223-238. doi:10.1007/s10265-014-0691-6
apa: Cires Rodriguez, E., & Prieto, J. (2015). Phylogenetic relationships of
Petrocoptis A. Braun ex Endl. (Caryophyllaceae), a discussed genus from the Iberian
Peninsula. Journal of Plant Research. Springer. https://doi.org/10.1007/s10265-014-0691-6
chicago: Cires Rodriguez, Eduardo, and José Prieto. “Phylogenetic Relationships
of Petrocoptis A. Braun Ex Endl. (Caryophyllaceae), a Discussed Genus from the
Iberian Peninsula.” Journal of Plant Research. Springer, 2015. https://doi.org/10.1007/s10265-014-0691-6.
ieee: E. Cires Rodriguez and J. Prieto, “Phylogenetic relationships of Petrocoptis
A. Braun ex Endl. (Caryophyllaceae), a discussed genus from the Iberian Peninsula,”
Journal of Plant Research, vol. 128, no. 2. Springer, pp. 223–238, 2015.
ista: Cires Rodriguez E, Prieto J. 2015. Phylogenetic relationships of Petrocoptis
A. Braun ex Endl. (Caryophyllaceae), a discussed genus from the Iberian Peninsula.
Journal of Plant Research. 128(2), 223–238.
mla: Cires Rodriguez, Eduardo, and José Prieto. “Phylogenetic Relationships of Petrocoptis
A. Braun Ex Endl. (Caryophyllaceae), a Discussed Genus from the Iberian Peninsula.”
Journal of Plant Research, vol. 128, no. 2, Springer, 2015, pp. 223–38,
doi:10.1007/s10265-014-0691-6.
short: E. Cires Rodriguez, J. Prieto, Journal of Plant Research 128 (2015) 223–238.
date_created: 2018-12-11T11:54:30Z
date_published: 2015-01-24T00:00:00Z
date_updated: 2021-01-12T06:53:47Z
day: '24'
department:
- _id: JiFr
doi: 10.1007/s10265-014-0691-6
intvolume: ' 128'
issue: '2'
language:
- iso: eng
month: '01'
oa_version: None
page: 223 - 238
publication: Journal of Plant Research
publication_status: published
publisher: Springer
publist_id: '5217'
quality_controlled: '1'
scopus_import: 1
status: public
title: Phylogenetic relationships of Petrocoptis A. Braun ex Endl. (Caryophyllaceae),
a discussed genus from the Iberian Peninsula
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 128
year: '2015'
...
---
_id: '1879'
abstract:
- lang: eng
text: When electron microscopy (EM) was introduced in the 1930s it gave scientists
their first look into the nanoworld of cells. Over the last 80 years EM has vastly
increased our understanding of the complex cellular structures that underlie the
diverse functions that cells need to maintain life. One drawback that has been
difficult to overcome was the inherent lack of volume information, mainly due
to the limit on the thickness of sections that could be viewed in a transmission
electron microscope (TEM). For many years scientists struggled to achieve three-dimensional
(3D) EM using serial section reconstructions, TEM tomography, and scanning EM
(SEM) techniques such as freeze-fracture. Although each technique yielded some
special information, they required a significant amount of time and specialist
expertise to obtain even a very small 3D EM dataset. Almost 20 years ago scientists
began to exploit SEMs to image blocks of embedded tissues and perform serial sectioning
of these tissues inside the SEM chamber. Using first focused ion beams (FIB) and
subsequently robotic ultramicrotomes (serial block-face, SBF-SEM) microscopists
were able to collect large volumes of 3D EM information at resolutions that could
address many important biological questions, and do so in an efficient manner.
We present here some examples of 3D EM taken from the many diverse specimens that
have been imaged in our core facility. We propose that the next major step forward
will be to efficiently correlate functional information obtained using light microscopy
(LM) with 3D EM datasets to more completely investigate the important links between
cell structures and their functions.
acknowledgement: The Zeiss Merlin with Gatan 3View2XP and Zeiss Auriga were acquired
through a CLEM grant from Minister Ingrid Lieten to the VIB Bio-Imaging-Core. Michiel
Krols and Saskia Lippens are the recipients of a fellowship from the FWO (Fonds
Wetenschappelijk Onderzoek) of Flanders.
author:
- first_name: A
full_name: Kremer, A
last_name: Kremer
- first_name: Stefaan
full_name: Lippens, Stefaan
last_name: Lippens
- first_name: Sonia
full_name: Bartunkova, Sonia
last_name: Bartunkova
- first_name: Bob
full_name: Asselbergh, Bob
last_name: Asselbergh
- first_name: Cendric
full_name: Blanpain, Cendric
last_name: Blanpain
- first_name: Matyas
full_name: Fendrych, Matyas
id: 43905548-F248-11E8-B48F-1D18A9856A87
last_name: Fendrych
orcid: 0000-0002-9767-8699
- first_name: A
full_name: Goossens, A
last_name: Goossens
- first_name: Matthew
full_name: Holt, Matthew
last_name: Holt
- first_name: Sophie
full_name: Janssens, Sophie
last_name: Janssens
- first_name: Michiel
full_name: Krols, Michiel
last_name: Krols
- first_name: Jean
full_name: Larsimont, Jean
last_name: Larsimont
- first_name: Conor
full_name: Mc Guire, Conor
last_name: Mc Guire
- first_name: Moritz
full_name: Nowack, Moritz
last_name: Nowack
- first_name: Xavier
full_name: Saelens, Xavier
last_name: Saelens
- first_name: Andreas
full_name: Schertel, Andreas
last_name: Schertel
- first_name: B
full_name: Schepens, B
last_name: Schepens
- first_name: M
full_name: Slezak, M
last_name: Slezak
- first_name: Vincent
full_name: Timmerman, Vincent
last_name: Timmerman
- first_name: Clara
full_name: Theunis, Clara
last_name: Theunis
- first_name: Ronald
full_name: Van Brempt, Ronald
last_name: Van Brempt
- first_name: Y
full_name: Visser, Y
last_name: Visser
- first_name: Christophe
full_name: Guérin, Christophe
last_name: Guérin
citation:
ama: Kremer A, Lippens S, Bartunkova S, et al. Developing 3D SEM in a broad biological
context. Journal of Microscopy. 2015;259(2):80-96. doi:10.1111/jmi.12211
apa: Kremer, A., Lippens, S., Bartunkova, S., Asselbergh, B., Blanpain, C., Fendrych,
M., … Guérin, C. (2015). Developing 3D SEM in a broad biological context. Journal
of Microscopy. Wiley-Blackwell. https://doi.org/10.1111/jmi.12211
chicago: Kremer, A, Stefaan Lippens, Sonia Bartunkova, Bob Asselbergh, Cendric Blanpain,
Matyas Fendrych, A Goossens, et al. “Developing 3D SEM in a Broad Biological Context.”
Journal of Microscopy. Wiley-Blackwell, 2015. https://doi.org/10.1111/jmi.12211.
ieee: A. Kremer et al., “Developing 3D SEM in a broad biological context,”
Journal of Microscopy, vol. 259, no. 2. Wiley-Blackwell, pp. 80–96, 2015.
ista: Kremer A, Lippens S, Bartunkova S, Asselbergh B, Blanpain C, Fendrych M, Goossens
A, Holt M, Janssens S, Krols M, Larsimont J, Mc Guire C, Nowack M, Saelens X,
Schertel A, Schepens B, Slezak M, Timmerman V, Theunis C, Van Brempt R, Visser
Y, Guérin C. 2015. Developing 3D SEM in a broad biological context. Journal of
Microscopy. 259(2), 80–96.
mla: Kremer, A., et al. “Developing 3D SEM in a Broad Biological Context.” Journal
of Microscopy, vol. 259, no. 2, Wiley-Blackwell, 2015, pp. 80–96, doi:10.1111/jmi.12211.
short: A. Kremer, S. Lippens, S. Bartunkova, B. Asselbergh, C. Blanpain, M. Fendrych,
A. Goossens, M. Holt, S. Janssens, M. Krols, J. Larsimont, C. Mc Guire, M. Nowack,
X. Saelens, A. Schertel, B. Schepens, M. Slezak, V. Timmerman, C. Theunis, R.
Van Brempt, Y. Visser, C. Guérin, Journal of Microscopy 259 (2015) 80–96.
date_created: 2018-12-11T11:54:30Z
date_published: 2015-08-01T00:00:00Z
date_updated: 2021-01-12T06:53:48Z
day: '01'
ddc:
- '570'
department:
- _id: JiFr
doi: 10.1111/jmi.12211
file:
- access_level: open_access
checksum: 3649c5372d1644062d728ea9287e367f
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:11:19Z
date_updated: 2020-07-14T12:45:19Z
file_id: '4872'
file_name: IST-2016-459-v1+1_KREMER_et_al-2015-Journal_of_Microscopy.pdf
file_size: 2899898
relation: main_file
file_date_updated: 2020-07-14T12:45:19Z
has_accepted_license: '1'
intvolume: ' 259'
issue: '2'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
page: 80 - 96
publication: Journal of Microscopy
publication_status: published
publisher: Wiley-Blackwell
publist_id: '5218'
pubrep_id: '459'
quality_controlled: '1'
scopus_import: 1
status: public
title: Developing 3D SEM in a broad biological context
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 259
year: '2015'
...
---
_id: '1944'
acknowledgement: This work was supported by the European Research Council (project
ERC-2011-StG-20101109-PSDP); the Agency for Innovation by Science and Technology
(IWT) (predoctoral fellowship to H.R.); and the People Programme (Marie Curie Actions)
of the European Union
author:
- first_name: Hana
full_name: Rakusová, Hana
last_name: Rakusová
- first_name: Matyas
full_name: Fendrych, Matyas
id: 43905548-F248-11E8-B48F-1D18A9856A87
last_name: Fendrych
orcid: 0000-0002-9767-8699
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Rakusová H, Fendrych M, Friml J. Intracellular trafficking and PIN-mediated
cell polarity during tropic responses in plants. Current Opinion in Plant Biology.
2015;23(2):116-123. doi:10.1016/j.pbi.2014.12.002
apa: Rakusová, H., Fendrych, M., & Friml, J. (2015). Intracellular trafficking
and PIN-mediated cell polarity during tropic responses in plants. Current Opinion
in Plant Biology. Elsevier. https://doi.org/10.1016/j.pbi.2014.12.002
chicago: Rakusová, Hana, Matyas Fendrych, and Jiří Friml. “Intracellular Trafficking
and PIN-Mediated Cell Polarity during Tropic Responses in Plants.” Current
Opinion in Plant Biology. Elsevier, 2015. https://doi.org/10.1016/j.pbi.2014.12.002.
ieee: H. Rakusová, M. Fendrych, and J. Friml, “Intracellular trafficking and PIN-mediated
cell polarity during tropic responses in plants,” Current Opinion in Plant
Biology, vol. 23, no. 2. Elsevier, pp. 116–123, 2015.
ista: Rakusová H, Fendrych M, Friml J. 2015. Intracellular trafficking and PIN-mediated
cell polarity during tropic responses in plants. Current Opinion in Plant Biology.
23(2), 116–123.
mla: Rakusová, Hana, et al. “Intracellular Trafficking and PIN-Mediated Cell Polarity
during Tropic Responses in Plants.” Current Opinion in Plant Biology, vol.
23, no. 2, Elsevier, 2015, pp. 116–23, doi:10.1016/j.pbi.2014.12.002.
short: H. Rakusová, M. Fendrych, J. Friml, Current Opinion in Plant Biology 23 (2015)
116–123.
date_created: 2018-12-11T11:54:51Z
date_published: 2015-02-01T00:00:00Z
date_updated: 2021-01-12T06:54:15Z
day: '01'
department:
- _id: JiFr
doi: 10.1016/j.pbi.2014.12.002
ec_funded: 1
intvolume: ' 23'
issue: '2'
language:
- iso: eng
month: '02'
oa_version: None
page: 116 - 123
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
- _id: 25681D80-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '291734'
name: International IST Postdoc Fellowship Programme
publication: Current Opinion in Plant Biology
publication_status: published
publisher: Elsevier
publist_id: '5140'
quality_controlled: '1'
scopus_import: 1
status: public
title: Intracellular trafficking and PIN-mediated cell polarity during tropic responses
in plants
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 23
year: '2015'
...
---
_id: '532'
abstract:
- lang: eng
text: Ethylene is a gaseous phytohormone that plays vital roles in plant growth
and development. Previous studies uncovered EIN2 as an essential signal transducer
linking ethylene perception on ER to transcriptional regulation in the nucleus
through a “cleave and shuttle” model. In this study, we report another mechanism
of EIN2-mediated ethylene signaling, whereby EIN2 imposes the translational repression
of EBF1 and EBF2 mRNA. We find that the EBF1/2 3′ UTRs mediate EIN2-directed translational
repression and identify multiple poly-uridylates (PolyU) motifs as functional
cis elements of 3′ UTRs. Furthermore, we demonstrate that ethylene induces EIN2
to associate with 3′ UTRs and target EBF1/2 mRNA to cytoplasmic processing-body
(P-body) through interacting with multiple P-body factors, including EIN5 and
PABs. Our study illustrates translational regulation as a key step in ethylene
signaling and presents mRNA 3′ UTR functioning as a “signal transducer” to sense
and relay cellular signaling in plants.
author:
- first_name: Wenyang
full_name: Li, Wenyang
last_name: Li
- first_name: Mengdi
full_name: Ma, Mengdi
last_name: Ma
- first_name: Ying
full_name: Feng, Ying
last_name: Feng
- first_name: Hongjiang
full_name: Li, Hongjiang
id: 33CA54A6-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0001-5039-9660
- first_name: Yichuan
full_name: Wang, Yichuan
last_name: Wang
- first_name: Yutong
full_name: Ma, Yutong
last_name: Ma
- first_name: Mingzhe
full_name: Li, Mingzhe
last_name: Li
- first_name: Fengying
full_name: An, Fengying
last_name: An
- first_name: Hongwei
full_name: Guo, Hongwei
last_name: Guo
citation:
ama: Li W, Ma M, Feng Y, et al. EIN2-directed translational regulation of ethylene
signaling in arabidopsis. Cell. 2015;163(3):670-683. doi:10.1016/j.cell.2015.09.037
apa: Li, W., Ma, M., Feng, Y., Li, H., Wang, Y., Ma, Y., … Guo, H. (2015). EIN2-directed
translational regulation of ethylene signaling in arabidopsis. Cell. Cell
Press. https://doi.org/10.1016/j.cell.2015.09.037
chicago: Li, Wenyang, Mengdi Ma, Ying Feng, Hongjiang Li, Yichuan Wang, Yutong Ma,
Mingzhe Li, Fengying An, and Hongwei Guo. “EIN2-Directed Translational Regulation
of Ethylene Signaling in Arabidopsis.” Cell. Cell Press, 2015. https://doi.org/10.1016/j.cell.2015.09.037.
ieee: W. Li et al., “EIN2-directed translational regulation of ethylene signaling
in arabidopsis,” Cell, vol. 163, no. 3. Cell Press, pp. 670–683, 2015.
ista: Li W, Ma M, Feng Y, Li H, Wang Y, Ma Y, Li M, An F, Guo H. 2015. EIN2-directed
translational regulation of ethylene signaling in arabidopsis. Cell. 163(3), 670–683.
mla: Li, Wenyang, et al. “EIN2-Directed Translational Regulation of Ethylene Signaling
in Arabidopsis.” Cell, vol. 163, no. 3, Cell Press, 2015, pp. 670–83, doi:10.1016/j.cell.2015.09.037.
short: W. Li, M. Ma, Y. Feng, H. Li, Y. Wang, Y. Ma, M. Li, F. An, H. Guo, Cell
163 (2015) 670–683.
date_created: 2018-12-11T11:47:00Z
date_published: 2015-10-22T00:00:00Z
date_updated: 2021-01-12T08:01:27Z
day: '22'
department:
- _id: JiFr
doi: 10.1016/j.cell.2015.09.037
intvolume: ' 163'
issue: '3'
language:
- iso: eng
month: '10'
oa_version: None
page: 670 - 683
publication: Cell
publication_status: published
publisher: Cell Press
publist_id: '7285'
quality_controlled: '1'
scopus_import: 1
status: public
title: EIN2-directed translational regulation of ethylene signaling in arabidopsis
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 163
year: '2015'
...
---
_id: '1509'
abstract:
- lang: eng
text: The Auxin Binding Protein1 (ABP1) has been identified based on its ability
to bind auxin with high affinity and studied for a long time as a prime candidate
for the extracellular auxin receptor responsible for mediating in particular the
fast non-transcriptional auxin responses. However, the contradiction between the
embryo-lethal phenotypes of the originally described Arabidopsis T-DNA insertional
knock-out alleles (abp1-1 and abp1-1s) and the wild type-like phenotypes of other
recently described loss-of-function alleles (abp1-c1 and abp1-TD1) questions the
biological importance of ABP1 and relevance of the previous genetic studies. Here
we show that there is no hidden copy of the ABP1 gene in the Arabidopsis genome
but the embryo-lethal phenotypes of abp1-1 and abp1-1s alleles are very similar
to the knock-out phenotypes of the neighboring gene, BELAYA SMERT (BSM). Furthermore,
the allelic complementation test between bsm and abp1 alleles shows that the embryo-lethality
in the abp1-1 and abp1-1s alleles is caused by the off-target disruption of the
BSM locus by the T-DNA insertions. This clarifies the controversy of different
phenotypes among published abp1 knock-out alleles and asks for reflections on
the developmental role of ABP1.
acknowledgement: "This work was supported by ERC Independent Research grant (ERC-2011-StG-20101109-PSDP
to JF). JM internship was supported by the grant “Action Austria – Slovakia”.\r\nData
associated with the article are available under the terms of the Creative Commons
Zero \"No rights reserved\" data waiver (CC0 1.0 Public domain dedication). \r\n\r\nData
availability: \r\nF1000Research: Dataset 1. Dataset 1, 10.5256/f1000research.7143.d104552\r\n\r\nF1000Research:
Dataset 2. Dataset 2, 10.5256/f1000research.7143.d104553\r\n\r\nF1000Research: Dataset
3. Dataset 3, 10.5256/f1000research.7143.d104554"
article_processing_charge: No
author:
- first_name: Jaroslav
full_name: Michalko, Jaroslav
id: 483727CA-F248-11E8-B48F-1D18A9856A87
last_name: Michalko
- first_name: Marta
full_name: Dravecka, Marta
id: 4342E402-F248-11E8-B48F-1D18A9856A87
last_name: Dravecka
orcid: 0000-0002-2519-8004
- first_name: Tobias
full_name: Bollenbach, Tobias
id: 3E6DB97A-F248-11E8-B48F-1D18A9856A87
last_name: Bollenbach
orcid: 0000-0003-4398-476X
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Michalko J, Lukacisinova M, Bollenbach MT, Friml J. Embryo-lethal phenotypes
in early abp1 mutants are due to disruption of the neighboring BSM gene. F1000
Research . 2015;4. doi:10.12688/f1000research.7143.1
apa: Michalko, J., Lukacisinova, M., Bollenbach, M. T., & Friml, J. (2015).
Embryo-lethal phenotypes in early abp1 mutants are due to disruption of the neighboring
BSM gene. F1000 Research . F1000 Research. https://doi.org/10.12688/f1000research.7143.1
chicago: Michalko, Jaroslav, Marta Lukacisinova, Mark Tobias Bollenbach, and Jiří
Friml. “Embryo-Lethal Phenotypes in Early Abp1 Mutants Are Due to Disruption of
the Neighboring BSM Gene.” F1000 Research . F1000 Research, 2015. https://doi.org/10.12688/f1000research.7143.1.
ieee: J. Michalko, M. Lukacisinova, M. T. Bollenbach, and J. Friml, “Embryo-lethal
phenotypes in early abp1 mutants are due to disruption of the neighboring BSM
gene,” F1000 Research , vol. 4. F1000 Research, 2015.
ista: Michalko J, Lukacisinova M, Bollenbach MT, Friml J. 2015. Embryo-lethal phenotypes
in early abp1 mutants are due to disruption of the neighboring BSM gene. F1000
Research . 4.
mla: Michalko, Jaroslav, et al. “Embryo-Lethal Phenotypes in Early Abp1 Mutants
Are Due to Disruption of the Neighboring BSM Gene.” F1000 Research , vol.
4, F1000 Research, 2015, doi:10.12688/f1000research.7143.1.
short: J. Michalko, M. Lukacisinova, M.T. Bollenbach, J. Friml, F1000 Research 4
(2015).
date_created: 2018-12-11T11:52:26Z
date_published: 2015-10-01T00:00:00Z
date_updated: 2025-05-07T11:12:30Z
day: '01'
ddc:
- '570'
department:
- _id: JiFr
- _id: ToBo
doi: 10.12688/f1000research.7143.1
ec_funded: 1
file:
- access_level: open_access
checksum: 8beae5cbe988e1060265ae7de2ee8306
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:16:12Z
date_updated: 2020-07-14T12:44:59Z
file_id: '5198'
file_name: IST-2016-497-v1+1_10.12688_f1000research.7143.1_20151102.pdf
file_size: 4414248
relation: main_file
file_date_updated: 2020-07-14T12:44:59Z
has_accepted_license: '1'
intvolume: ' 4'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: 'F1000 Research '
publication_status: published
publisher: F1000 Research
publist_id: '5668'
pubrep_id: '497'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Embryo-lethal phenotypes in early abp1 mutants are due to disruption of the
neighboring BSM gene
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 4
year: '2015'
...
---
_id: '1532'
abstract:
- lang: eng
text: Ammonium is the major nitrogen source in some plant ecosystems but is toxic
at high concentrations, especially when available as the exclusive nitrogen source.
Ammonium stress rapidly leads to various metabolic and hormonal imbalances that
ultimately inhibit root and shoot growth in many plant species, including Arabidopsis
thaliana (L.) Heynh. To identify molecular and genetic factors involved in seedling
survival with prolonged exclusive NH4+ nutrition, a transcriptomic analysis with
microarrays was used. Substantial transcriptional differences were most pronounced
in (NH4)2SO4-grown seedlings, compared with plants grown on KNO3 or NH4NO3. Consistent
with previous physiological analyses, major differences in the expression modules
of photosynthesis-related genes, an altered mitochondrial metabolism, differential
expression of the primary NH4+ assimilation, alteration of transporter gene expression
and crucial changes in cell wall biosynthesis were found. A major difference in
plant hormone responses, particularly of auxin but not cytokinin, was striking.
The activity of the DR5::GUS reporter revealed a dramatically decreased auxin
response in (NH4)2SO4-grown primary roots. The impaired root growth on (NH4)2SO4
was partially rescued by exogenous auxin or in specific mutants in the auxin pathway.
The data suggest that NH4+-induced nutritional and metabolic imbalances can be
partially overcome by elevated auxin levels.
article_processing_charge: No
article_type: original
author:
- first_name: Huaiyu
full_name: Yang, Huaiyu
last_name: Yang
- first_name: Jenny
full_name: Von Der Fecht Bartenbach, Jenny
last_name: Von Der Fecht Bartenbach
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Jan
full_name: Lohmann, Jan
last_name: Lohmann
- first_name: Benjamin
full_name: Neuhäuser, Benjamin
last_name: Neuhäuser
- first_name: Uwe
full_name: Ludewig, Uwe
last_name: Ludewig
citation:
ama: Yang H, Von Der Fecht Bartenbach J, Friml J, Lohmann J, Neuhäuser B, Ludewig
U. Auxin-modulated root growth inhibition in Arabidopsis thaliana seedlings with
ammonium as the sole nitrogen source. Functional Plant Biology. 2015;42(3):239-251.
doi:10.1071/FP14171
apa: Yang, H., Von Der Fecht Bartenbach, J., Friml, J., Lohmann, J., Neuhäuser,
B., & Ludewig, U. (2015). Auxin-modulated root growth inhibition in Arabidopsis
thaliana seedlings with ammonium as the sole nitrogen source. Functional Plant
Biology. CSIRO. https://doi.org/10.1071/FP14171
chicago: Yang, Huaiyu, Jenny Von Der Fecht Bartenbach, Jiří Friml, Jan Lohmann,
Benjamin Neuhäuser, and Uwe Ludewig. “Auxin-Modulated Root Growth Inhibition in
Arabidopsis Thaliana Seedlings with Ammonium as the Sole Nitrogen Source.” Functional
Plant Biology. CSIRO, 2015. https://doi.org/10.1071/FP14171.
ieee: H. Yang, J. Von Der Fecht Bartenbach, J. Friml, J. Lohmann, B. Neuhäuser,
and U. Ludewig, “Auxin-modulated root growth inhibition in Arabidopsis thaliana
seedlings with ammonium as the sole nitrogen source,” Functional Plant Biology,
vol. 42, no. 3. CSIRO, pp. 239–251, 2015.
ista: Yang H, Von Der Fecht Bartenbach J, Friml J, Lohmann J, Neuhäuser B, Ludewig
U. 2015. Auxin-modulated root growth inhibition in Arabidopsis thaliana seedlings
with ammonium as the sole nitrogen source. Functional Plant Biology. 42(3), 239–251.
mla: Yang, Huaiyu, et al. “Auxin-Modulated Root Growth Inhibition in Arabidopsis
Thaliana Seedlings with Ammonium as the Sole Nitrogen Source.” Functional Plant
Biology, vol. 42, no. 3, CSIRO, 2015, pp. 239–51, doi:10.1071/FP14171.
short: H. Yang, J. Von Der Fecht Bartenbach, J. Friml, J. Lohmann, B. Neuhäuser,
U. Ludewig, Functional Plant Biology 42 (2015) 239–251.
date_created: 2018-12-11T11:52:34Z
date_published: 2015-03-01T00:00:00Z
date_updated: 2022-05-24T09:02:24Z
day: '01'
department:
- _id: JiFr
doi: 10.1071/FP14171
external_id:
pmid:
- '32480670'
intvolume: ' 42'
issue: '3'
language:
- iso: eng
month: '03'
oa_version: None
page: 239 - 251
pmid: 1
publication: Functional Plant Biology
publication_identifier:
issn:
- 1445-4408
publication_status: published
publisher: CSIRO
publist_id: '5639'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Auxin-modulated root growth inhibition in Arabidopsis thaliana seedlings with
ammonium as the sole nitrogen source
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 42
year: '2015'
...
---
_id: '1534'
abstract:
- lang: eng
text: PIN proteins are auxin export carriers that direct intercellular auxin flow
and in turn regulate many aspects of plant growth and development including responses
to environmental changes. The Arabidopsis R2R3-MYB transcription factor FOUR LIPS
(FLP) and its paralogue MYB88 regulate terminal divisions during stomatal development,
as well as female reproductive development and stress responses. Here we show
that FLP and MYB88 act redundantly but differentially in regulating the transcription
of PIN3 and PIN7 in gravity-sensing cells of primary and lateral roots. On the
one hand, FLP is involved in responses to gravity stimulation in primary roots,
whereas on the other, FLP and MYB88 function complementarily in establishing the
gravitropic set-point angles of lateral roots. Our results support a model in
which FLP and MYB88 expression specifically determines the temporal-spatial patterns
of PIN3 and PIN7 transcription that are closely associated with their preferential
functions during root responses to gravity.
article_number: '8822'
author:
- first_name: Hongzhe
full_name: Wang, Hongzhe
last_name: Wang
- first_name: Kezhen
full_name: Yang, Kezhen
last_name: Yang
- first_name: Junjie
full_name: Zou, Junjie
last_name: Zou
- first_name: Lingling
full_name: Zhu, Lingling
last_name: Zhu
- first_name: Zidian
full_name: Xie, Zidian
last_name: Xie
- first_name: Miyoterao
full_name: Morita, Miyoterao
last_name: Morita
- first_name: Masao
full_name: Tasaka, Masao
last_name: Tasaka
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Erich
full_name: Grotewold, Erich
last_name: Grotewold
- first_name: Tom
full_name: Beeckman, Tom
last_name: Beeckman
- first_name: Steffen
full_name: Vanneste, Steffen
last_name: Vanneste
- first_name: Fred
full_name: Sack, Fred
last_name: Sack
- first_name: Jie
full_name: Le, Jie
last_name: Le
citation:
ama: Wang H, Yang K, Zou J, et al. Transcriptional regulation of PIN genes by FOUR
LIPS and MYB88 during Arabidopsis root gravitropism. Nature Communications.
2015;6. doi:10.1038/ncomms9822
apa: Wang, H., Yang, K., Zou, J., Zhu, L., Xie, Z., Morita, M., … Le, J. (2015).
Transcriptional regulation of PIN genes by FOUR LIPS and MYB88 during Arabidopsis
root gravitropism. Nature Communications. Nature Publishing Group. https://doi.org/10.1038/ncomms9822
chicago: Wang, Hongzhe, Kezhen Yang, Junjie Zou, Lingling Zhu, Zidian Xie, Miyoterao
Morita, Masao Tasaka, et al. “Transcriptional Regulation of PIN Genes by FOUR
LIPS and MYB88 during Arabidopsis Root Gravitropism.” Nature Communications.
Nature Publishing Group, 2015. https://doi.org/10.1038/ncomms9822.
ieee: H. Wang et al., “Transcriptional regulation of PIN genes by FOUR LIPS
and MYB88 during Arabidopsis root gravitropism,” Nature Communications,
vol. 6. Nature Publishing Group, 2015.
ista: Wang H, Yang K, Zou J, Zhu L, Xie Z, Morita M, Tasaka M, Friml J, Grotewold
E, Beeckman T, Vanneste S, Sack F, Le J. 2015. Transcriptional regulation of PIN
genes by FOUR LIPS and MYB88 during Arabidopsis root gravitropism. Nature Communications.
6, 8822.
mla: Wang, Hongzhe, et al. “Transcriptional Regulation of PIN Genes by FOUR LIPS
and MYB88 during Arabidopsis Root Gravitropism.” Nature Communications,
vol. 6, 8822, Nature Publishing Group, 2015, doi:10.1038/ncomms9822.
short: H. Wang, K. Yang, J. Zou, L. Zhu, Z. Xie, M. Morita, M. Tasaka, J. Friml,
E. Grotewold, T. Beeckman, S. Vanneste, F. Sack, J. Le, Nature Communications
6 (2015).
date_created: 2018-12-11T11:52:34Z
date_published: 2015-11-18T00:00:00Z
date_updated: 2021-01-12T06:51:26Z
day: '18'
ddc:
- '570'
department:
- _id: JiFr
doi: 10.1038/ncomms9822
ec_funded: 1
file:
- access_level: open_access
checksum: 3c06735fc7cd7e482ca830cbd26001bf
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:17:07Z
date_updated: 2020-07-14T12:45:01Z
file_id: '5259'
file_name: IST-2016-485-v1+1_ncomms9822.pdf
file_size: 1852268
relation: main_file
file_date_updated: 2020-07-14T12:45:01Z
has_accepted_license: '1'
intvolume: ' 6'
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: Nature Communications
publication_status: published
publisher: Nature Publishing Group
publist_id: '5637'
pubrep_id: '485'
quality_controlled: '1'
scopus_import: 1
status: public
title: Transcriptional regulation of PIN genes by FOUR LIPS and MYB88 during Arabidopsis
root gravitropism
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 6
year: '2015'
...
---
_id: '1536'
abstract:
- lang: eng
text: Strigolactones, first discovered as germination stimulants for parasitic weeds
[1], are carotenoid-derived phytohormones that play major roles in inhibiting
lateral bud outgrowth and promoting plant-mycorrhizal symbiosis [2-4]. Furthermore,
strigolactones are involved in the regulation of lateral and adventitious root
development, root cell division [5, 6], secondary growth [7], and leaf senescence
[8]. Recently, we discovered the strigolactone transporter Petunia axillaris PLEIOTROPIC
DRUG RESISTANCE 1 (PaPDR1), which is required for efficient mycorrhizal colonization
and inhibition of lateral bud outgrowth [9]. However, how strigolactones are transported
through the plant remained unknown. Here we show that PaPDR1 exhibits a cell-type-specific
asymmetric localization in different root tissues. In root tips, PaPDR1 is co-expressed
with the strigolactone biosynthetic gene DAD1 (CCD8), and it is localized at the
apical membrane of root hypodermal cells, presumably mediating the shootward transport
of strigolactone. Above the root tip, in the hypodermal passage cells that form
gates for the entry of mycorrhizal fungi, PaPDR1 is present in the outer-lateral
membrane, compatible with its postulated function as strigolactone exporter from
root to soil. Transport studies are in line with our localization studies since
(1) a papdr1 mutant displays impaired transport of strigolactones out of the root
tip to the shoot as well as into the rhizosphere and (2) DAD1 expression and PIN1/PIN2
levels change in plants deregulated for PDR1 expression, suggestive of variations
in endogenous strigolactone contents. In conclusion, our results indicate that
the polar localizations of PaPDR1 mediate directional shootward strigolactone
transport as well as localized exudation into the soil.
acknowledgement: "This work was funded by a grant of the Swiss National Foundation
to E.M.\r\nWe thank Dr. José María Mateos (University of Zurich) for providing us
with the vibratome, Prof. Dolf Weijers (Wageningen University, the Netherlands)
for shipping us his set of ligation-independent cloning vectors, Prof. Bruno Humbel
(University of Lausanne) for suggestions on GFP-PDR1 detection, and Dr. Undine Krügel
(University of Zurich) and Prof. Michal Jasinski (Polish Academy of Science) for
hints on protein quantification."
author:
- first_name: Joëlle
full_name: Sasse, Joëlle
last_name: Sasse
- first_name: Sibu
full_name: Simon, Sibu
id: 4542EF9A-F248-11E8-B48F-1D18A9856A87
last_name: Simon
orcid: 0000-0002-1998-6741
- first_name: Christian
full_name: Gübeli, Christian
last_name: Gübeli
- first_name: Guowei
full_name: Liu, Guowei
last_name: Liu
- first_name: Xi
full_name: Cheng, Xi
last_name: Cheng
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Harro
full_name: Bouwmeester, Harro
last_name: Bouwmeester
- first_name: Enrico
full_name: Martinoia, Enrico
last_name: Martinoia
- first_name: Lorenzo
full_name: Borghi, Lorenzo
last_name: Borghi
citation:
ama: Sasse J, Simon S, Gübeli C, et al. Asymmetric localizations of the ABC transporter
PaPDR1 trace paths of directional strigolactone transport. Current Biology.
2015;25(5):647-655. doi:10.1016/j.cub.2015.01.015
apa: Sasse, J., Simon, S., Gübeli, C., Liu, G., Cheng, X., Friml, J., … Borghi,
L. (2015). Asymmetric localizations of the ABC transporter PaPDR1 trace paths
of directional strigolactone transport. Current Biology. Cell Press. https://doi.org/10.1016/j.cub.2015.01.015
chicago: Sasse, Joëlle, Sibu Simon, Christian Gübeli, Guowei Liu, Xi Cheng, Jiří
Friml, Harro Bouwmeester, Enrico Martinoia, and Lorenzo Borghi. “Asymmetric Localizations
of the ABC Transporter PaPDR1 Trace Paths of Directional Strigolactone Transport.”
Current Biology. Cell Press, 2015. https://doi.org/10.1016/j.cub.2015.01.015.
ieee: J. Sasse et al., “Asymmetric localizations of the ABC transporter PaPDR1
trace paths of directional strigolactone transport,” Current Biology, vol.
25, no. 5. Cell Press, pp. 647–655, 2015.
ista: Sasse J, Simon S, Gübeli C, Liu G, Cheng X, Friml J, Bouwmeester H, Martinoia
E, Borghi L. 2015. Asymmetric localizations of the ABC transporter PaPDR1 trace
paths of directional strigolactone transport. Current Biology. 25(5), 647–655.
mla: Sasse, Joëlle, et al. “Asymmetric Localizations of the ABC Transporter PaPDR1
Trace Paths of Directional Strigolactone Transport.” Current Biology, vol.
25, no. 5, Cell Press, 2015, pp. 647–55, doi:10.1016/j.cub.2015.01.015.
short: J. Sasse, S. Simon, C. Gübeli, G. Liu, X. Cheng, J. Friml, H. Bouwmeester,
E. Martinoia, L. Borghi, Current Biology 25 (2015) 647–655.
date_created: 2018-12-11T11:52:35Z
date_published: 2015-02-12T00:00:00Z
date_updated: 2021-01-12T06:51:27Z
day: '12'
department:
- _id: JiFr
doi: 10.1016/j.cub.2015.01.015
intvolume: ' 25'
issue: '5'
language:
- iso: eng
month: '02'
oa_version: None
page: 647 - 655
publication: Current Biology
publication_status: published
publisher: Cell Press
publist_id: '5635'
quality_controlled: '1'
scopus_import: 1
status: public
title: Asymmetric localizations of the ABC transporter PaPDR1 trace paths of directional
strigolactone transport
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 25
year: '2015'
...
---
_id: '1543'
abstract:
- lang: eng
text: A plethora of diverse programmed cell death (PCD) processes has been described
in living organisms. In animals and plants, different forms of PCD play crucial
roles in development, immunity, and responses to the environment. While the molecular
control of some animal PCD forms such as apoptosis is known in great detail, we
still know comparatively little about the regulation of the diverse types of plant
PCD. In part, this deficiency in molecular understanding is caused by the lack
of reliable reporters to detect PCD processes. Here, we addressed this issue by
using a combination of bioinformatics approaches to identify commonly regulated
genes during diverse plant PCD processes in Arabidopsis (Arabidopsis thaliana).
Our results indicate that the transcriptional signatures of developmentally controlled
cell death are largely distinct from the ones associated with environmentally
induced cell death. Moreover, different cases of developmental PCD share a set
of cell death-associated genes. Most of these genes are evolutionary conserved
within the green plant lineage, arguing for an evolutionary conserved core machinery
of developmental PCD. Based on this information, we established an array of specific
promoter-reporter lines for developmental PCD in Arabidopsis. These PCD indicators
represent a powerful resource that can be used in addition to established morphological
and biochemical methods to detect and analyze PCD processes in vivo and in planta.
author:
- first_name: Yadira
full_name: Olvera Carrillo, Yadira
last_name: Olvera Carrillo
- first_name: Michiel
full_name: Van Bel, Michiel
last_name: Van Bel
- first_name: Tom
full_name: Van Hautegem, Tom
last_name: Van Hautegem
- first_name: Matyas
full_name: Fendrych, Matyas
id: 43905548-F248-11E8-B48F-1D18A9856A87
last_name: Fendrych
orcid: 0000-0002-9767-8699
- first_name: Marlies
full_name: Huysmans, Marlies
last_name: Huysmans
- first_name: Mária
full_name: Šimášková, Mária
last_name: Šimášková
- first_name: Matthias
full_name: Van Durme, Matthias
last_name: Van Durme
- first_name: Pierre
full_name: Buscaill, Pierre
last_name: Buscaill
- first_name: Susana
full_name: Rivas, Susana
last_name: Rivas
- first_name: Núria
full_name: Coll, Núria
last_name: Coll
- first_name: Frederik
full_name: Coppens, Frederik
last_name: Coppens
- first_name: Steven
full_name: Maere, Steven
last_name: Maere
- first_name: Moritz
full_name: Nowack, Moritz
last_name: Nowack
citation:
ama: Olvera Carrillo Y, Van Bel M, Van Hautegem T, et al. A conserved core of programmed
cell death indicator genes discriminates developmentally and environmentally induced
programmed cell death in plants. Plant Physiology. 2015;169(4):2684-2699.
doi:10.1104/pp.15.00769
apa: Olvera Carrillo, Y., Van Bel, M., Van Hautegem, T., Fendrych, M., Huysmans,
M., Šimášková, M., … Nowack, M. (2015). A conserved core of programmed cell death
indicator genes discriminates developmentally and environmentally induced programmed
cell death in plants. Plant Physiology. American Society of Plant Biologists.
https://doi.org/10.1104/pp.15.00769
chicago: Olvera Carrillo, Yadira, Michiel Van Bel, Tom Van Hautegem, Matyas Fendrych,
Marlies Huysmans, Mária Šimášková, Matthias Van Durme, et al. “A Conserved Core
of Programmed Cell Death Indicator Genes Discriminates Developmentally and Environmentally
Induced Programmed Cell Death in Plants.” Plant Physiology. American Society
of Plant Biologists, 2015. https://doi.org/10.1104/pp.15.00769.
ieee: Y. Olvera Carrillo et al., “A conserved core of programmed cell death
indicator genes discriminates developmentally and environmentally induced programmed
cell death in plants,” Plant Physiology, vol. 169, no. 4. American Society
of Plant Biologists, pp. 2684–2699, 2015.
ista: Olvera Carrillo Y, Van Bel M, Van Hautegem T, Fendrych M, Huysmans M, Šimášková
M, Van Durme M, Buscaill P, Rivas S, Coll N, Coppens F, Maere S, Nowack M. 2015.
A conserved core of programmed cell death indicator genes discriminates developmentally
and environmentally induced programmed cell death in plants. Plant Physiology.
169(4), 2684–2699.
mla: Olvera Carrillo, Yadira, et al. “A Conserved Core of Programmed Cell Death
Indicator Genes Discriminates Developmentally and Environmentally Induced Programmed
Cell Death in Plants.” Plant Physiology, vol. 169, no. 4, American Society
of Plant Biologists, 2015, pp. 2684–99, doi:10.1104/pp.15.00769.
short: Y. Olvera Carrillo, M. Van Bel, T. Van Hautegem, M. Fendrych, M. Huysmans,
M. Šimášková, M. Van Durme, P. Buscaill, S. Rivas, N. Coll, F. Coppens, S. Maere,
M. Nowack, Plant Physiology 169 (2015) 2684–2699.
date_created: 2018-12-11T11:52:38Z
date_published: 2015-12-01T00:00:00Z
date_updated: 2021-01-12T06:51:30Z
day: '01'
department:
- _id: JiFr
doi: 10.1104/pp.15.00769
intvolume: ' 169'
issue: '4'
language:
- iso: eng
month: '12'
oa_version: None
page: 2684 - 2699
publication: Plant Physiology
publication_status: published
publisher: American Society of Plant Biologists
publist_id: '5628'
scopus_import: 1
status: public
title: A conserved core of programmed cell death indicator genes discriminates developmentally
and environmentally induced programmed cell death in plants
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 169
year: '2015'
...
---
_id: '1554'
abstract:
- lang: eng
text: The visualization of hormonal signaling input and output is key to understanding
how multicellular development is regulated. The plant signaling molecule auxin
triggers many growth and developmental responses, but current tools lack the sensitivity
or precision to visualize these. We developed a set of fluorescent reporters that
allow sensitive and semiquantitative readout of auxin responses at cellular resolution
in Arabidopsis thaliana. These generic tools are suitable for any transformable
plant species.
author:
- first_name: Cheyang
full_name: Liao, Cheyang
last_name: Liao
- first_name: Wouter
full_name: Smet, Wouter
last_name: Smet
- first_name: Géraldine
full_name: Brunoud, Géraldine
last_name: Brunoud
- first_name: Saiko
full_name: Yoshida, Saiko
id: 2E46069C-F248-11E8-B48F-1D18A9856A87
last_name: Yoshida
- first_name: Teva
full_name: Vernoux, Teva
last_name: Vernoux
- first_name: Dolf
full_name: Weijers, Dolf
last_name: Weijers
citation:
ama: Liao C, Smet W, Brunoud G, Yoshida S, Vernoux T, Weijers D. Reporters for sensitive
and quantitative measurement of auxin response. Nature Methods. 2015;12(3):207-210.
doi:10.1038/nmeth.3279
apa: Liao, C., Smet, W., Brunoud, G., Yoshida, S., Vernoux, T., & Weijers, D.
(2015). Reporters for sensitive and quantitative measurement of auxin response.
Nature Methods. Nature Publishing Group. https://doi.org/10.1038/nmeth.3279
chicago: Liao, Cheyang, Wouter Smet, Géraldine Brunoud, Saiko Yoshida, Teva Vernoux,
and Dolf Weijers. “Reporters for Sensitive and Quantitative Measurement of Auxin
Response.” Nature Methods. Nature Publishing Group, 2015. https://doi.org/10.1038/nmeth.3279.
ieee: C. Liao, W. Smet, G. Brunoud, S. Yoshida, T. Vernoux, and D. Weijers, “Reporters
for sensitive and quantitative measurement of auxin response,” Nature Methods,
vol. 12, no. 3. Nature Publishing Group, pp. 207–210, 2015.
ista: Liao C, Smet W, Brunoud G, Yoshida S, Vernoux T, Weijers D. 2015. Reporters
for sensitive and quantitative measurement of auxin response. Nature Methods.
12(3), 207–210.
mla: Liao, Cheyang, et al. “Reporters for Sensitive and Quantitative Measurement
of Auxin Response.” Nature Methods, vol. 12, no. 3, Nature Publishing Group,
2015, pp. 207–10, doi:10.1038/nmeth.3279.
short: C. Liao, W. Smet, G. Brunoud, S. Yoshida, T. Vernoux, D. Weijers, Nature
Methods 12 (2015) 207–210.
date_created: 2018-12-11T11:52:41Z
date_published: 2015-02-26T00:00:00Z
date_updated: 2021-01-12T06:51:34Z
day: '26'
department:
- _id: JiFr
doi: 10.1038/nmeth.3279
external_id:
pmid:
- '25643149'
intvolume: ' 12'
issue: '3'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4344836/
month: '02'
oa: 1
oa_version: Submitted Version
page: 207 - 210
pmid: 1
publication: Nature Methods
publication_status: published
publisher: Nature Publishing Group
publist_id: '5617'
quality_controlled: '1'
scopus_import: 1
status: public
title: Reporters for sensitive and quantitative measurement of auxin response
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 12
year: '2015'
...
---
_id: '1556'
abstract:
- lang: eng
text: The elongator complex subunit 2 (ELP2) protein, one subunit of an evolutionarily
conserved histone acetyltransferase complex, has been shown to participate in
leaf patterning, plant immune and abiotic stress responses in Arabidopsis thaliana.
Here, its role in root development was explored. Compared to the wild type, the
elp2 mutant exhibited an accelerated differentiation of its root stem cells and
cell division was more active in its quiescent centre (QC). The key transcription
factors responsible for maintaining root stem cell and QC identity, such as AP2
transcription factors PLT1 (PLETHORA1) and PLT2 (PLETHORA2), GRAS transcription
factors such as SCR (SCARECROW) and SHR (SHORT ROOT) and WUSCHEL-RELATED HOMEOBOX5
transcription factor WOX5, were all strongly down-regulated in the mutant. On
the other hand, expression of the G2/M transition activator CYCB1 was substantially
induced in elp2. The auxin efflux transporters PIN1 and PIN2 showed decreased
protein levels and PIN1 also displayed mild polarity alterations in elp2, which
resulted in a reduced auxin content in the root tip. Either the acetylation or
methylation level of each of these genes differed between the mutant and the wild
type, suggesting that the ELP2 regulation of root development involves the epigenetic
modification of a range of transcription factors and other developmental regulators.
author:
- first_name: Yuebin
full_name: Jia, Yuebin
last_name: Jia
- first_name: Huiyu
full_name: Tian, Huiyu
last_name: Tian
- first_name: Hongjiang
full_name: Li, Hongjiang
id: 33CA54A6-F248-11E8-B48F-1D18A9856A87
last_name: Li
orcid: 0000-0001-5039-9660
- first_name: Qianqian
full_name: Yu, Qianqian
last_name: Yu
- first_name: Lei
full_name: Wang, Lei
last_name: Wang
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Zhaojun
full_name: Ding, Zhaojun
last_name: Ding
citation:
ama: Jia Y, Tian H, Li H, et al. The Arabidopsis thaliana elongator complex subunit
2 epigenetically affects root development. Journal of Experimental Botany.
2015;66(15):4631-4642. doi:10.1093/jxb/erv230
apa: Jia, Y., Tian, H., Li, H., Yu, Q., Wang, L., Friml, J., & Ding, Z. (2015).
The Arabidopsis thaliana elongator complex subunit 2 epigenetically affects root
development. Journal of Experimental Botany. Oxford University Press. https://doi.org/10.1093/jxb/erv230
chicago: Jia, Yuebin, Huiyu Tian, Hongjiang Li, Qianqian Yu, Lei Wang, Jiří Friml,
and Zhaojun Ding. “The Arabidopsis Thaliana Elongator Complex Subunit 2 Epigenetically
Affects Root Development.” Journal of Experimental Botany. Oxford University
Press, 2015. https://doi.org/10.1093/jxb/erv230.
ieee: Y. Jia et al., “The Arabidopsis thaliana elongator complex subunit
2 epigenetically affects root development,” Journal of Experimental Botany,
vol. 66, no. 15. Oxford University Press, pp. 4631–4642, 2015.
ista: Jia Y, Tian H, Li H, Yu Q, Wang L, Friml J, Ding Z. 2015. The Arabidopsis
thaliana elongator complex subunit 2 epigenetically affects root development.
Journal of Experimental Botany. 66(15), 4631–4642.
mla: Jia, Yuebin, et al. “The Arabidopsis Thaliana Elongator Complex Subunit 2 Epigenetically
Affects Root Development.” Journal of Experimental Botany, vol. 66, no.
15, Oxford University Press, 2015, pp. 4631–42, doi:10.1093/jxb/erv230.
short: Y. Jia, H. Tian, H. Li, Q. Yu, L. Wang, J. Friml, Z. Ding, Journal of Experimental
Botany 66 (2015) 4631–4642.
date_created: 2018-12-11T11:52:42Z
date_published: 2015-08-01T00:00:00Z
date_updated: 2021-01-12T06:51:35Z
day: '01'
ddc:
- '570'
department:
- _id: JiFr
doi: 10.1093/jxb/erv230
file:
- access_level: open_access
checksum: 257919be0ce3d306185d3891ad7acf39
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:14:02Z
date_updated: 2020-07-14T12:45:02Z
file_id: '5051'
file_name: IST-2016-480-v1+1_J._Exp._Bot.-2015-Jia-4631-42.pdf
file_size: 7753043
relation: main_file
file_date_updated: 2020-07-14T12:45:02Z
has_accepted_license: '1'
intvolume: ' 66'
issue: '15'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
page: 4631 - 4642
publication: Journal of Experimental Botany
publication_status: published
publisher: Oxford University Press
publist_id: '5615'
pubrep_id: '480'
quality_controlled: '1'
scopus_import: 1
status: public
title: The Arabidopsis thaliana elongator complex subunit 2 epigenetically affects
root development
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 66
year: '2015'
...
---
_id: '1558'
abstract:
- lang: eng
text: CyclophilinAis a conserved peptidyl-prolyl cis-trans isomerase (PPIase) best
known as the cellular receptor of the immunosuppressant cyclosporine A. Despite
significant effort, evidence of developmental functions of cyclophilin A in non-plant
systems has remained obscure. Mutations in a tomato (Solanum lycopersicum) cyclophilin
A ortholog, DIAGEOTROPICA (DGT), have been shown to abolish the organogenesis
of lateral roots; however, a mechanistic explanation of the phenotype is lacking.
Here, we show that the dgt mutant lacks auxin maxima relevant to priming and specification
of lateral root founder cells. DGT is expressed in shoot and root, and localizes
to both the nucleus and cytoplasm during lateral root organogenesis. Mutation
of ENTIRE/ IAA9, a member of the auxin-responsive Aux/IAA protein family of transcriptional
repressors, partially restores the inability of dgt to initiate lateral root primordia
but not the primordia outgrowth. By comparison, grafting of a wild-type scion
restores the process of lateral root formation, consistent with participation
of a mobile signal. Antibodies do not detect movement of the DGT protein into
the dgt rootstock; however, experiments with radiolabeled auxin and an auxin-specific
microelectrode demonstrate abnormal auxin fluxes. Functional studies of DGT in
heterologous yeast and tobacco-leaf auxin-transport systems demonstrate that DGT
negatively regulates PIN-FORMED (PIN) auxin efflux transporters by affecting their
plasma membrane localization. Studies in tomato support complex effects of the
dgt mutation on PIN expression level, expression domain and plasma membrane localization.
Our data demonstrate that DGT regulates auxin transport in lateral root formation.
author:
- first_name: Maria
full_name: Ivanchenko, Maria
last_name: Ivanchenko
- first_name: Jinsheng
full_name: Zhu, Jinsheng
last_name: Zhu
- first_name: Bangjun
full_name: Wang, Bangjun
last_name: Wang
- first_name: Eva
full_name: Medvecka, Eva
id: 298814E2-F248-11E8-B48F-1D18A9856A87
last_name: Medvecka
- first_name: Yunlong
full_name: Du, Yunlong
last_name: Du
- first_name: Elisa
full_name: Azzarello, Elisa
last_name: Azzarello
- first_name: Stefano
full_name: Mancuso, Stefano
last_name: Mancuso
- first_name: Molly
full_name: Megraw, Molly
last_name: Megraw
- first_name: Sergei
full_name: Filichkin, Sergei
last_name: Filichkin
- first_name: Joseph
full_name: Dubrovsky, Joseph
last_name: Dubrovsky
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Markus
full_name: Geisler, Markus
last_name: Geisler
citation:
ama: Ivanchenko M, Zhu J, Wang B, et al. The cyclophilin a DIAGEOTROPICA gene affects
auxin transport in both root and shoot to control lateral root formation. Development.
2015;142(4):712-721. doi:10.1242/dev.113225
apa: Ivanchenko, M., Zhu, J., Wang, B., Medvecka, E., Du, Y., Azzarello, E., … Geisler,
M. (2015). The cyclophilin a DIAGEOTROPICA gene affects auxin transport in both
root and shoot to control lateral root formation. Development. Company
of Biologists. https://doi.org/10.1242/dev.113225
chicago: Ivanchenko, Maria, Jinsheng Zhu, Bangjun Wang, Eva Medvecka, Yunlong Du,
Elisa Azzarello, Stefano Mancuso, et al. “The Cyclophilin a DIAGEOTROPICA Gene
Affects Auxin Transport in Both Root and Shoot to Control Lateral Root Formation.”
Development. Company of Biologists, 2015. https://doi.org/10.1242/dev.113225.
ieee: M. Ivanchenko et al., “The cyclophilin a DIAGEOTROPICA gene affects
auxin transport in both root and shoot to control lateral root formation,” Development,
vol. 142, no. 4. Company of Biologists, pp. 712–721, 2015.
ista: Ivanchenko M, Zhu J, Wang B, Medvecka E, Du Y, Azzarello E, Mancuso S, Megraw
M, Filichkin S, Dubrovsky J, Friml J, Geisler M. 2015. The cyclophilin a DIAGEOTROPICA
gene affects auxin transport in both root and shoot to control lateral root formation.
Development. 142(4), 712–721.
mla: Ivanchenko, Maria, et al. “The Cyclophilin a DIAGEOTROPICA Gene Affects Auxin
Transport in Both Root and Shoot to Control Lateral Root Formation.” Development,
vol. 142, no. 4, Company of Biologists, 2015, pp. 712–21, doi:10.1242/dev.113225.
short: M. Ivanchenko, J. Zhu, B. Wang, E. Medvecka, Y. Du, E. Azzarello, S. Mancuso,
M. Megraw, S. Filichkin, J. Dubrovsky, J. Friml, M. Geisler, Development 142 (2015)
712–721.
date_created: 2018-12-11T11:52:42Z
date_published: 2015-02-15T00:00:00Z
date_updated: 2021-01-12T06:51:35Z
day: '15'
department:
- _id: JiFr
doi: 10.1242/dev.113225
intvolume: ' 142'
issue: '4'
language:
- iso: eng
month: '02'
oa_version: None
page: 712 - 721
publication: Development
publication_status: published
publisher: Company of Biologists
publist_id: '5613'
quality_controlled: '1'
scopus_import: 1
status: public
title: The cyclophilin a DIAGEOTROPICA gene affects auxin transport in both root and
shoot to control lateral root formation
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 142
year: '2015'
...