---
_id: '6269'
abstract:
- lang: eng
text: 'Clathrin-Mediated Endocytosis (CME) is an aspect of cellular trafficking
that is constantly regulated for mediating developmental and physiological responses.
The main aim of my thesis is to decipher the basic mechanisms of CME and post-endocytic
trafficking in the whole multicellular organ systems of Arabidopsis. The first
chapter of my thesis describes the search for new components involved in CME.
Tandem affinity purification was conducted using CLC and its interacting partners
were identified. Amongst the identified proteins were the Auxilin-likes1 and 2
(Axl1/2), putative uncoating factors, for which we made a full functional analysis.
Over-expression of Axl1/2 causes extreme modifications in the dynamics of the
machinery proteins and inhibition of endocytosis altogether. However the loss
of function of the axl1/2 did not present any cellular or physiological phenotype,
meaning Auxilin-likes do not form the major uncoating machinery. The second chapter
of my thesis describes the establishment/utilisation of techniques to capture
the dynamicity and the complexity of CME and post-endocytic trafficking. We have
studied the development of endocytic pits at the PM – specifically, the mode of
membrane remodeling during pit development and the role of actin in it, given
plant cells possess high turgor pressure. Utilizing the improved z-resolution
of TIRF and VAEM techniques, we captured the time-lapse of the endocytic events
at the plasma membrane; and using particle detection software, we quantitatively
analysed all the endocytic trajectories in an unbiased way to obtain the endocytic
rate of the system. This together with the direct analysis of cargo internalisation
from the PM provided an estimate on the endocytic potential of the cell. We also
developed a methodology for ultrastructural analysis of different populations
of Clathrin-Coated Structures (CCSs) in both PM and endomembranes in unroofed
protoplasts. Structural analysis, together with the intensity profile of CCSs
at the PM show that the mode of CCP development at the PM follows ‘Constant curvature
model’; meaning that clathrin polymerisation energy is a major contributing factor
of membrane remodeling. In addition, other analyses clearly show that actin is
not required for membrane remodeling during invagination or any other step of
CCP development, despite the prevalent high turgor pressure. However, actin is
essential in orchestrating the post-endocytic trafficking of CCVs facilitating
the EE formation. We also observed that the uncoating process post-endocytosis
is not immediate; an alternative mechanism of uncoating – Sequential multi-step
process – functions in the cell. Finally we also looked at one of the important
physiological stimuli modulating the process – hormone, auxin. auxin has been
known to influence CME before. We have made a detailed study on the concentration-time
based effect of auxin on the machinery proteins, CCP development, and the specificity
of cargoes endocytosed. To this end, we saw no general effect of auxin on CME
at earlier time points. However, very low concentration of IAA, such as 50nM,
accelerates endocytosis of specifically PIN2 through CME. Such a tight regulatory
control with high specificity to PIN2 could be essential in modulating its polarity. '
acknowledged_ssus:
- _id: Bio
- _id: EM-Fac
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Madhumitha
full_name: Narasimhan, Madhumitha
id: 44BF24D0-F248-11E8-B48F-1D18A9856A87
last_name: Narasimhan
orcid: 0000-0002-8600-0671
citation:
ama: Narasimhan M. Clathrin-Mediated endocytosis, post-endocytic trafficking and
their regulatory controls in plants . 2019. doi:10.15479/at:ista:th1075
apa: Narasimhan, M. (2019). Clathrin-Mediated endocytosis, post-endocytic trafficking
and their regulatory controls in plants . Institute of Science and Technology
Austria. https://doi.org/10.15479/at:ista:th1075
chicago: Narasimhan, Madhumitha. “Clathrin-Mediated Endocytosis, Post-Endocytic
Trafficking and Their Regulatory Controls in Plants .” Institute of Science and
Technology Austria, 2019. https://doi.org/10.15479/at:ista:th1075.
ieee: M. Narasimhan, “Clathrin-Mediated endocytosis, post-endocytic trafficking
and their regulatory controls in plants ,” Institute of Science and Technology
Austria, 2019.
ista: Narasimhan M. 2019. Clathrin-Mediated endocytosis, post-endocytic trafficking
and their regulatory controls in plants . Institute of Science and Technology
Austria.
mla: Narasimhan, Madhumitha. Clathrin-Mediated Endocytosis, Post-Endocytic Trafficking
and Their Regulatory Controls in Plants . Institute of Science and Technology
Austria, 2019, doi:10.15479/at:ista:th1075.
short: M. Narasimhan, Clathrin-Mediated Endocytosis, Post-Endocytic Trafficking
and Their Regulatory Controls in Plants , Institute of Science and Technology
Austria, 2019.
date_created: 2019-04-09T14:37:06Z
date_published: 2019-02-04T00:00:00Z
date_updated: 2025-05-07T11:12:27Z
day: '04'
ddc:
- '575'
degree_awarded: PhD
department:
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issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
related_material:
record:
- id: '412'
relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
title: 'Clathrin-Mediated endocytosis, post-endocytic trafficking and their regulatory
controls in plants '
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2019'
...
---
_id: '6351'
abstract:
- lang: eng
text: "A process of restorative patterning in plant roots correctly replaces eliminated
cells to heal local injuries despite the absence of cell migration, which underpins
wound healing in animals. \r\n\r\nPatterning in plants relies on oriented cell
divisions and acquisition of specific cell identities. Plants regularly endure
wounds caused by abiotic or biotic environmental stimuli and have developed extraordinary
abilities to restore their tissues after injuries. Here, we provide insight into
a mechanism of restorative patterning that repairs tissues after wounding. Laser-assisted
elimination of different cells in Arabidopsis root combined with live-imaging
tracking during vertical growth allowed analysis of the regeneration processes
in vivo. Specifically, the cells adjacent to the inner side of the injury re-activated
their stem cell transcriptional programs. They accelerated their progression through
cell cycle, coordinately changed the cell division orientation, and ultimately
acquired de novo the correct cell fates to replace missing cells. These observations
highlight existence of unknown intercellular positional signaling and demonstrate
the capability of specified cells to re-acquire stem cell programs as a crucial
part of the plant-specific mechanism of wound healing."
acknowledged_ssus:
- _id: Bio
article_processing_charge: No
author:
- first_name: Petra
full_name: Marhavá, Petra
id: 44E59624-F248-11E8-B48F-1D18A9856A87
last_name: Marhavá
- first_name: Lukas
full_name: Hörmayer, Lukas
id: 2EEE7A2A-F248-11E8-B48F-1D18A9856A87
last_name: Hörmayer
orcid: 0000-0001-8295-2926
- first_name: Saiko
full_name: Yoshida, Saiko
id: 2E46069C-F248-11E8-B48F-1D18A9856A87
last_name: Yoshida
- first_name: Peter
full_name: Marhavy, Peter
id: 3F45B078-F248-11E8-B48F-1D18A9856A87
last_name: Marhavy
orcid: 0000-0001-5227-5741
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Marhavá P, Hörmayer L, Yoshida S, Marhavý P, Benková E, Friml J. Re-activation
of stem cell pathways for pattern restoration in plant wound healing. Cell.
2019;177(4):957-969.e13. doi:10.1016/j.cell.2019.04.015
apa: Marhavá, P., Hörmayer, L., Yoshida, S., Marhavý, P., Benková, E., & Friml,
J. (2019). Re-activation of stem cell pathways for pattern restoration in plant
wound healing. Cell. Elsevier. https://doi.org/10.1016/j.cell.2019.04.015
chicago: Marhavá, Petra, Lukas Hörmayer, Saiko Yoshida, Peter Marhavý, Eva Benková,
and Jiří Friml. “Re-Activation of Stem Cell Pathways for Pattern Restoration in
Plant Wound Healing.” Cell. Elsevier, 2019. https://doi.org/10.1016/j.cell.2019.04.015.
ieee: P. Marhavá, L. Hörmayer, S. Yoshida, P. Marhavý, E. Benková, and J. Friml,
“Re-activation of stem cell pathways for pattern restoration in plant wound healing,”
Cell, vol. 177, no. 4. Elsevier, p. 957–969.e13, 2019.
ista: Marhavá P, Hörmayer L, Yoshida S, Marhavý P, Benková E, Friml J. 2019. Re-activation
of stem cell pathways for pattern restoration in plant wound healing. Cell. 177(4),
957–969.e13.
mla: Marhavá, Petra, et al. “Re-Activation of Stem Cell Pathways for Pattern Restoration
in Plant Wound Healing.” Cell, vol. 177, no. 4, Elsevier, 2019, p. 957–969.e13,
doi:10.1016/j.cell.2019.04.015.
short: P. Marhavá, L. Hörmayer, S. Yoshida, P. Marhavý, E. Benková, J. Friml, Cell
177 (2019) 957–969.e13.
date_created: 2019-04-28T21:59:14Z
date_published: 2019-05-02T00:00:00Z
date_updated: 2024-03-25T23:30:06Z
day: '02'
ddc:
- '570'
department:
- _id: JiFr
- _id: EvBe
doi: 10.1016/j.cell.2019.04.015
ec_funded: 1
external_id:
isi:
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page: 957-969.e13
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project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Cell
publication_identifier:
eissn:
- '10974172'
issn:
- '00928674'
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
link:
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relation: press_release
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record:
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status: public
scopus_import: '1'
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title: Re-activation of stem cell pathways for pattern restoration in plant wound
healing
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 177
year: '2019'
...
---
_id: '6366'
abstract:
- lang: eng
text: Plants have a remarkable capacity to adjust their growth and development to
elevated ambient temperatures. Increased elongation growth of roots, hypocotyls
and petioles in warm temperatures are hallmarks of seedling thermomorphogenesis.
In the last decade, significant progress has been made to identify the molecular
signaling components regulating these growth responses. Increased ambient temperature
utilizes diverse components of the light sensing and signal transduction network
to trigger growth adjustments. However, it remains unknown whether temperature
sensing and responses are universal processes that occur uniformly in all plant
organs. Alternatively, temperature sensing may be confined to specific tissues
or organs, which would require a systemic signal that mediates responses in distal
parts of the plant. Here we show that Arabidopsis (Arabidopsis thaliana) seedlings
show organ-specific transcriptome responses to elevated temperatures, and that
thermomorphogenesis involves both autonomous and organ-interdependent temperature
sensing and signaling. Seedling roots can sense and respond to temperature in
a shoot-independent manner, whereas shoot temperature responses require both local
and systemic processes. The induction of cell elongation in hypocotyls requires
temperature sensing in cotyledons, followed by generation of a mobile auxin signal.
Subsequently, auxin travels to the hypocotyl where it triggers local brassinosteroid-induced
cell elongation in seedling stems, which depends upon a distinct, permissive temperature
sensor in the hypocotyl.
article_processing_charge: No
article_type: original
author:
- first_name: Julia
full_name: Bellstaedt, Julia
last_name: Bellstaedt
- first_name: Jana
full_name: Trenner, Jana
last_name: Trenner
- first_name: Rebecca
full_name: Lippmann, Rebecca
last_name: Lippmann
- first_name: Yvonne
full_name: Poeschl, Yvonne
last_name: Poeschl
- first_name: Xixi
full_name: Zhang, Xixi
id: 61A66458-47E9-11EA-85BA-8AEAAF14E49A
last_name: Zhang
orcid: 0000-0001-7048-4627
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Marcel
full_name: Quint, Marcel
last_name: Quint
- first_name: Carolin
full_name: Delker, Carolin
last_name: Delker
citation:
ama: Bellstaedt J, Trenner J, Lippmann R, et al. A mobile auxin signal connects
temperature sensing in cotyledons with growth responses in hypocotyls. Plant
Physiology. 2019;180(2):757-766. doi:10.1104/pp.18.01377
apa: Bellstaedt, J., Trenner, J., Lippmann, R., Poeschl, Y., Zhang, X., Friml, J.,
… Delker, C. (2019). A mobile auxin signal connects temperature sensing in cotyledons
with growth responses in hypocotyls. Plant Physiology. ASPB. https://doi.org/10.1104/pp.18.01377
chicago: Bellstaedt, Julia, Jana Trenner, Rebecca Lippmann, Yvonne Poeschl, Xixi
Zhang, Jiří Friml, Marcel Quint, and Carolin Delker. “A Mobile Auxin Signal Connects
Temperature Sensing in Cotyledons with Growth Responses in Hypocotyls.” Plant
Physiology. ASPB, 2019. https://doi.org/10.1104/pp.18.01377.
ieee: J. Bellstaedt et al., “A mobile auxin signal connects temperature sensing
in cotyledons with growth responses in hypocotyls,” Plant Physiology, vol.
180, no. 2. ASPB, pp. 757–766, 2019.
ista: Bellstaedt J, Trenner J, Lippmann R, Poeschl Y, Zhang X, Friml J, Quint M,
Delker C. 2019. A mobile auxin signal connects temperature sensing in cotyledons
with growth responses in hypocotyls. Plant Physiology. 180(2), 757–766.
mla: Bellstaedt, Julia, et al. “A Mobile Auxin Signal Connects Temperature Sensing
in Cotyledons with Growth Responses in Hypocotyls.” Plant Physiology, vol.
180, no. 2, ASPB, 2019, pp. 757–66, doi:10.1104/pp.18.01377.
short: J. Bellstaedt, J. Trenner, R. Lippmann, Y. Poeschl, X. Zhang, J. Friml, M.
Quint, C. Delker, Plant Physiology 180 (2019) 757–766.
date_created: 2019-04-30T15:24:22Z
date_published: 2019-06-01T00:00:00Z
date_updated: 2023-09-05T12:25:19Z
day: '01'
department:
- _id: JiFr
doi: 10.1104/pp.18.01377
external_id:
isi:
- '000470086100019'
pmid:
- '31000634'
intvolume: ' 180'
isi: 1
issue: '2'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: www.doi.org/10.1104/pp.18.01377
month: '06'
oa: 1
oa_version: Published Version
page: 757-766
pmid: 1
publication: Plant Physiology
publication_identifier:
eissn:
- 1532-2548
issn:
- 0032-0889
publication_status: published
publisher: ASPB
quality_controlled: '1'
scopus_import: '1'
status: public
title: A mobile auxin signal connects temperature sensing in cotyledons with growth
responses in hypocotyls
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 180
year: '2019'
...
---
_id: '6377'
abstract:
- lang: eng
text: Clathrin-mediated endocytosis (CME) is a highly conserved and essential cellular
process in eukaryotic cells, but its dynamic and vital nature makes it challenging
to study using classical genetics tools. In contrast, although small molecules
can acutely and reversibly perturb CME, the few chemical CME inhibitors that have
been applied to plants are either ineffective or show undesirable side effects.
Here, we identify the previously described endosidin9 (ES9) as an inhibitor of
clathrin heavy chain (CHC) function in both Arabidopsis and human cells through
affinity-based target isolation, in vitro binding studies and X-ray crystallography.
Moreover, we present a chemically improved ES9 analog, ES9-17, which lacks the
undesirable side effects of ES9 while retaining the ability to target CHC. ES9
and ES9-17 have expanded the chemical toolbox used to probe CHC function, and
present chemical scaffolds for further design of more specific and potent CHC
inhibitors across different systems.
article_processing_charge: No
article_type: original
author:
- first_name: Wim
full_name: Dejonghe, Wim
last_name: Dejonghe
- first_name: Isha
full_name: Sharma, Isha
last_name: Sharma
- first_name: Bram
full_name: Denoo, Bram
last_name: Denoo
- first_name: Steven
full_name: De Munck, Steven
last_name: De Munck
- first_name: Qing
full_name: Lu, Qing
last_name: Lu
- first_name: Kiril
full_name: Mishev, Kiril
last_name: Mishev
- first_name: Haydar
full_name: Bulut, Haydar
last_name: Bulut
- first_name: Evelien
full_name: Mylle, Evelien
last_name: Mylle
- first_name: Riet
full_name: De Rycke, Riet
last_name: De Rycke
- first_name: Mina K
full_name: Vasileva, Mina K
id: 3407EB18-F248-11E8-B48F-1D18A9856A87
last_name: Vasileva
- first_name: Daniel V.
full_name: Savatin, Daniel V.
last_name: Savatin
- first_name: Wim
full_name: Nerinckx, Wim
last_name: Nerinckx
- first_name: An
full_name: Staes, An
last_name: Staes
- first_name: Andrzej
full_name: Drozdzecki, Andrzej
last_name: Drozdzecki
- first_name: Dominique
full_name: Audenaert, Dominique
last_name: Audenaert
- first_name: Klaas
full_name: Yperman, Klaas
last_name: Yperman
- first_name: Annemieke
full_name: Madder, Annemieke
last_name: Madder
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Daniël
full_name: Van Damme, Daniël
last_name: Van Damme
- first_name: Kris
full_name: Gevaert, Kris
last_name: Gevaert
- first_name: Volker
full_name: Haucke, Volker
last_name: Haucke
- first_name: Savvas N.
full_name: Savvides, Savvas N.
last_name: Savvides
- first_name: Johan
full_name: Winne, Johan
last_name: Winne
- first_name: Eugenia
full_name: Russinova, Eugenia
last_name: Russinova
citation:
ama: Dejonghe W, Sharma I, Denoo B, et al. Disruption of endocytosis through chemical
inhibition of clathrin heavy chain function. Nature Chemical Biology. 2019;15(6):641–649.
doi:10.1038/s41589-019-0262-1
apa: Dejonghe, W., Sharma, I., Denoo, B., De Munck, S., Lu, Q., Mishev, K., … Russinova,
E. (2019). Disruption of endocytosis through chemical inhibition of clathrin heavy
chain function. Nature Chemical Biology. Springer Nature. https://doi.org/10.1038/s41589-019-0262-1
chicago: Dejonghe, Wim, Isha Sharma, Bram Denoo, Steven De Munck, Qing Lu, Kiril
Mishev, Haydar Bulut, et al. “Disruption of Endocytosis through Chemical Inhibition
of Clathrin Heavy Chain Function.” Nature Chemical Biology. Springer Nature,
2019. https://doi.org/10.1038/s41589-019-0262-1.
ieee: W. Dejonghe et al., “Disruption of endocytosis through chemical inhibition
of clathrin heavy chain function,” Nature Chemical Biology, vol. 15, no.
6. Springer Nature, pp. 641–649, 2019.
ista: Dejonghe W, Sharma I, Denoo B, De Munck S, Lu Q, Mishev K, Bulut H, Mylle
E, De Rycke R, Vasileva MK, Savatin DV, Nerinckx W, Staes A, Drozdzecki A, Audenaert
D, Yperman K, Madder A, Friml J, Van Damme D, Gevaert K, Haucke V, Savvides SN,
Winne J, Russinova E. 2019. Disruption of endocytosis through chemical inhibition
of clathrin heavy chain function. Nature Chemical Biology. 15(6), 641–649.
mla: Dejonghe, Wim, et al. “Disruption of Endocytosis through Chemical Inhibition
of Clathrin Heavy Chain Function.” Nature Chemical Biology, vol. 15, no.
6, Springer Nature, 2019, pp. 641–649, doi:10.1038/s41589-019-0262-1.
short: W. Dejonghe, I. Sharma, B. Denoo, S. De Munck, Q. Lu, K. Mishev, H. Bulut,
E. Mylle, R. De Rycke, M.K. Vasileva, D.V. Savatin, W. Nerinckx, A. Staes, A.
Drozdzecki, D. Audenaert, K. Yperman, A. Madder, J. Friml, D. Van Damme, K. Gevaert,
V. Haucke, S.N. Savvides, J. Winne, E. Russinova, Nature Chemical Biology 15 (2019)
641–649.
date_created: 2019-05-05T21:59:11Z
date_published: 2019-06-01T00:00:00Z
date_updated: 2023-09-07T12:54:35Z
day: '01'
department:
- _id: JiFr
doi: 10.1038/s41589-019-0262-1
external_id:
isi:
- '000468195600018'
intvolume: ' 15'
isi: 1
issue: '6'
language:
- iso: eng
month: '06'
oa_version: None
page: 641–649
publication: Nature Chemical Biology
publication_identifier:
eissn:
- '15524469'
issn:
- '15524450'
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
record:
- id: '7172'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Disruption of endocytosis through chemical inhibition of clathrin heavy chain
function
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 15
year: '2019'
...
---
_id: '6504'
abstract:
- lang: eng
text: "Root gravitropism is one of the most important processes allowing plant adaptation
to the land environment. Auxin plays a central role in mediating root gravitropism,
but how auxin contributes to gravitational perception and the subsequent response
is still unclear.\r\n\r\nHere, we showed that the local auxin maximum/gradient
within the root apex, which is generated by the PIN directional auxin transporters,
regulates the expression of three key starch granule synthesis genes, SS4, PGM
and ADG1, which in turn influence the accumulation of starch granules that serve
as a statolith perceiving gravity.\r\n\r\nMoreover, using the cvxIAA‐ccvTIR1 system,
we also showed that TIR1‐mediated auxin signaling is required for starch granule
formation and gravitropic response within root tips. In addition, axr3 mutants
showed reduced auxin‐mediated starch granule accumulation and disruption of gravitropism
within the root apex.\r\n\r\nOur results indicate that auxin‐mediated statolith
production relies on the TIR1/AFB‐AXR3‐mediated auxin signaling pathway. In summary,
we propose a dual role for auxin in gravitropism: the regulation of both gravity
perception and response."
article_processing_charge: No
article_type: original
author:
- first_name: Yuzhou
full_name: Zhang, Yuzhou
id: 3B6137F2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
orcid: 0000-0003-2627-6956
- first_name: P
full_name: He, P
last_name: He
- first_name: X
full_name: Ma, X
last_name: Ma
- first_name: Z
full_name: Yang, Z
last_name: Yang
- first_name: C
full_name: Pang, C
last_name: Pang
- first_name: J
full_name: Yu, J
last_name: Yu
- first_name: G
full_name: Wang, G
last_name: Wang
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: G
full_name: Xiao, G
last_name: Xiao
citation:
ama: Zhang Y, He P, Ma X, et al. Auxin-mediated statolith production for root gravitropism.
New Phytologist. 2019;224(2):761-774. doi:10.1111/nph.15932
apa: Zhang, Y., He, P., Ma, X., Yang, Z., Pang, C., Yu, J., … Xiao, G. (2019). Auxin-mediated
statolith production for root gravitropism. New Phytologist. Wiley. https://doi.org/10.1111/nph.15932
chicago: Zhang, Yuzhou, P He, X Ma, Z Yang, C Pang, J Yu, G Wang, Jiří Friml, and
G Xiao. “Auxin-Mediated Statolith Production for Root Gravitropism.” New Phytologist.
Wiley, 2019. https://doi.org/10.1111/nph.15932.
ieee: Y. Zhang et al., “Auxin-mediated statolith production for root gravitropism,”
New Phytologist, vol. 224, no. 2. Wiley, pp. 761–774, 2019.
ista: Zhang Y, He P, Ma X, Yang Z, Pang C, Yu J, Wang G, Friml J, Xiao G. 2019.
Auxin-mediated statolith production for root gravitropism. New Phytologist. 224(2),
761–774.
mla: Zhang, Yuzhou, et al. “Auxin-Mediated Statolith Production for Root Gravitropism.”
New Phytologist, vol. 224, no. 2, Wiley, 2019, pp. 761–74, doi:10.1111/nph.15932.
short: Y. Zhang, P. He, X. Ma, Z. Yang, C. Pang, J. Yu, G. Wang, J. Friml, G. Xiao,
New Phytologist 224 (2019) 761–774.
date_created: 2019-05-28T14:33:26Z
date_published: 2019-10-01T00:00:00Z
date_updated: 2023-08-28T08:40:13Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1111/nph.15932
external_id:
isi:
- '000487184200024'
pmid:
- '31111487'
file:
- access_level: open_access
checksum: 6488243334538f5c39099a701cbf76b9
content_type: application/pdf
creator: dernst
date_created: 2020-10-14T08:59:33Z
date_updated: 2020-10-14T08:59:33Z
file_id: '8661'
file_name: 2019_NewPhytologist_Zhang_accepted.pdf
file_size: 1099061
relation: main_file
success: 1
file_date_updated: 2020-10-14T08:59:33Z
has_accepted_license: '1'
intvolume: ' 224'
isi: 1
issue: '2'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Submitted Version
page: 761-774
pmid: 1
publication: New Phytologist
publication_identifier:
eissn:
- 1469-8137
issn:
- 0028-646x
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Auxin-mediated statolith production for root gravitropism
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 224
year: '2019'
...
---
_id: '280'
abstract:
- lang: eng
text: Flowers have a species-specific functional life span that determines the time
window in which pollination, fertilization and seed set can occur. The stigma
tissue plays a key role in flower receptivity by intercepting pollen and initiating
pollen tube growth toward the ovary. In this article, we show that a developmentally
controlled cell death programme terminates the functional life span of stigma
cells in Arabidopsis. We identified the leaf senescence regulator ORESARA1 (also
known as ANAC092) and the previously uncharacterized KIRA1 (also known as ANAC074)
as partially redundant transcription factors that modulate stigma longevity by
controlling the expression of programmed cell death-associated genes. KIRA1 expression
is sufficient to induce cell death and terminate floral receptivity, whereas lack
of both KIRA1 and ORESARA1 substantially increases stigma life span. Surprisingly,
the extension of stigma longevity is accompanied by only a moderate extension
of flower receptivity, suggesting that additional processes participate in the
control of the flower's receptive life span.
acknowledgement: We gratefully acknowledge funding from the Chinese Scholarship Council
(CSC; project number 201206910025 to Z.G.), the Fonds Wetenschappelijk Onderzoek
(FWO; project number G005112N to A.D.; fellowship number 12I7417N to Z.L.), the
Belgian Federal Science Policy Office (BELSPO; to Y.S.), the Agency for Innovation
by Science and Technology of Belgium (IWT; fellowship number 121110 to M.V.D.),
the Hercules foundation (grant AUGE-09-029 to K.D.), and the ERC StG PROCELLDEATH
(project number 639234 to M.K.N.).
article_processing_charge: No
author:
- first_name: Zhen
full_name: Gao, Zhen
last_name: Gao
- first_name: Anna
full_name: Daneva, Anna
last_name: Daneva
- first_name: Yuliya
full_name: Salanenka, Yuliya
id: 46DAAE7E-F248-11E8-B48F-1D18A9856A87
last_name: Salanenka
- first_name: Matthias
full_name: Van Durme, Matthias
last_name: Van Durme
- first_name: Marlies
full_name: Huysmans, Marlies
last_name: Huysmans
- first_name: Zongcheng
full_name: Lin, Zongcheng
last_name: Lin
- first_name: Freya
full_name: De Winter, Freya
last_name: De Winter
- first_name: Steffen
full_name: Vanneste, Steffen
last_name: Vanneste
- first_name: Mansour
full_name: Karimi, Mansour
last_name: Karimi
- first_name: Jan
full_name: Van De Velde, Jan
last_name: Van De Velde
- first_name: Klaas
full_name: Vandepoele, Klaas
last_name: Vandepoele
- first_name: Davy
full_name: Van De Walle, Davy
last_name: Van De Walle
- first_name: Koen
full_name: Dewettinck, Koen
last_name: Dewettinck
- first_name: Bart
full_name: Lambrecht, Bart
last_name: Lambrecht
- first_name: Moritz
full_name: Nowack, Moritz
last_name: Nowack
citation:
ama: Gao Z, Daneva A, Salanenka Y, et al. KIRA1 and ORESARA1 terminate flower receptivity
by promoting cell death in the stigma of Arabidopsis. Nature Plants. 2018;4(6):365-375.
doi:10.1038/s41477-018-0160-7
apa: Gao, Z., Daneva, A., Salanenka, Y., Van Durme, M., Huysmans, M., Lin, Z., …
Nowack, M. (2018). KIRA1 and ORESARA1 terminate flower receptivity by promoting
cell death in the stigma of Arabidopsis. Nature Plants. Nature Publishing
Group. https://doi.org/10.1038/s41477-018-0160-7
chicago: Gao, Zhen, Anna Daneva, Yuliya Salanenka, Matthias Van Durme, Marlies Huysmans,
Zongcheng Lin, Freya De Winter, et al. “KIRA1 and ORESARA1 Terminate Flower Receptivity
by Promoting Cell Death in the Stigma of Arabidopsis.” Nature Plants. Nature
Publishing Group, 2018. https://doi.org/10.1038/s41477-018-0160-7.
ieee: Z. Gao et al., “KIRA1 and ORESARA1 terminate flower receptivity by
promoting cell death in the stigma of Arabidopsis,” Nature Plants, vol.
4, no. 6. Nature Publishing Group, pp. 365–375, 2018.
ista: Gao Z, Daneva A, Salanenka Y, Van Durme M, Huysmans M, Lin Z, De Winter F,
Vanneste S, Karimi M, Van De Velde J, Vandepoele K, Van De Walle D, Dewettinck
K, Lambrecht B, Nowack M. 2018. KIRA1 and ORESARA1 terminate flower receptivity
by promoting cell death in the stigma of Arabidopsis. Nature Plants. 4(6), 365–375.
mla: Gao, Zhen, et al. “KIRA1 and ORESARA1 Terminate Flower Receptivity by Promoting
Cell Death in the Stigma of Arabidopsis.” Nature Plants, vol. 4, no. 6,
Nature Publishing Group, 2018, pp. 365–75, doi:10.1038/s41477-018-0160-7.
short: Z. Gao, A. Daneva, Y. Salanenka, M. Van Durme, M. Huysmans, Z. Lin, F. De
Winter, S. Vanneste, M. Karimi, J. Van De Velde, K. Vandepoele, D. Van De Walle,
K. Dewettinck, B. Lambrecht, M. Nowack, Nature Plants 4 (2018) 365–375.
date_created: 2018-12-11T11:45:35Z
date_published: 2018-05-28T00:00:00Z
date_updated: 2023-09-13T08:24:17Z
day: '28'
department:
- _id: JiFr
doi: 10.1038/s41477-018-0160-7
external_id:
isi:
- '000435571000017'
intvolume: ' 4'
isi: 1
issue: '6'
language:
- iso: eng
month: '05'
oa_version: None
page: 365 - 375
publication: Nature Plants
publication_status: published
publisher: Nature Publishing Group
publist_id: '7619'
quality_controlled: '1'
scopus_import: '1'
status: public
title: KIRA1 and ORESARA1 terminate flower receptivity by promoting cell death in
the stigma of Arabidopsis
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 4
year: '2018'
...
---
_id: '10881'
abstract:
- lang: eng
text: Strigolactones (SLs) are a relatively recent addition to the list of plant
hormones that control different aspects of plant development. SL signalling is
perceived by an α/β hydrolase, DWARF 14 (D14). A close homolog of D14, KARRIKIN
INSENSTIVE2 (KAI2), is involved in perception of an uncharacterized molecule called
karrikin (KAR). Recent studies in Arabidopsis identified the SUPPRESSOR OF MAX2
1 (SMAX1) and SMAX1-LIKE 7 (SMXL7) to be potential SCF–MAX2 complex-mediated proteasome
targets of KAI2 and D14, respectively. Genetic studies on SMXL7 and SMAX1 demonstrated
distinct developmental roles for each, but very little is known about these repressors
in terms of their sequence features. In this study, we performed an extensive
comparative analysis of SMXLs and determined their phylogenetic and evolutionary
history in the plant lineage. Our results show that SMXL family members can be
sub-divided into four distinct phylogenetic clades/classes, with an ancient SMAX1.
Further, we identified the clade-specific motifs that have evolved and that might
act as determinants of SL-KAR signalling specificity. These specificities resulted
from functional diversities among the clades. Our results suggest that a gradual
co-evolution of SMXL members with their upstream receptors D14/KAI2 provided an
increased specificity to both the SL perception and response in land plants.
acknowledgement: "This project received funding from the European Union’s Horizon
2020 research and innovation programme under the Marie Skłodowska-Curie Actions
and it is co-financed by the South Moravian Region under grant agreement No. 665860
(SS). Access to computing and storage facilities owned by parties and projects contributing
to the national grid infrastructure, MetaCentrum, provided under the program ‘Projects
of Large Infrastructure for Research, Development, and Innovations’ (LM2010005)
was greatly appreciated (RSV). The project was funded by The Ministry of Education,
Youth and Sports/MES of the Czech Republic under the project CEITEC 2020 (LQ1601)
(TN, TRM). JF was supported by the European Research Council (project ERC-2011-StG
20101109-PSDP) and the Czech Science Foundation GAČR (GA13-40637S). We thank Dr
Kamel Chibani for active discussions on the evolutionary analysis and Nandan Mysore
Vardarajan for his critical comments on the manuscript. This article reflects\r\nonly
the authors’ views, and the EU is not responsible for any use that may be made of
the information it contains. "
article_processing_charge: No
article_type: original
author:
- first_name: Taraka Ramji
full_name: Moturu, Taraka Ramji
last_name: Moturu
- first_name: Sravankumar
full_name: Thula, Sravankumar
last_name: Thula
- first_name: Ravi Kumar
full_name: Singh, Ravi Kumar
last_name: Singh
- first_name: Tomasz
full_name: Nodzyński, Tomasz
last_name: Nodzyński
- first_name: Radka Svobodová
full_name: Vařeková, Radka Svobodová
last_name: Vařeková
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Sibu
full_name: Simon, Sibu
last_name: Simon
citation:
ama: Moturu TR, Thula S, Singh RK, et al. Molecular evolution and diversification
of the SMXL gene family. Journal of Experimental Botany. 2018;69(9):2367-2378.
doi:10.1093/jxb/ery097
apa: Moturu, T. R., Thula, S., Singh, R. K., Nodzyński, T., Vařeková, R. S., Friml,
J., & Simon, S. (2018). Molecular evolution and diversification of the SMXL
gene family. Journal of Experimental Botany. Oxford University Press. https://doi.org/10.1093/jxb/ery097
chicago: Moturu, Taraka Ramji, Sravankumar Thula, Ravi Kumar Singh, Tomasz Nodzyński,
Radka Svobodová Vařeková, Jiří Friml, and Sibu Simon. “Molecular Evolution and
Diversification of the SMXL Gene Family.” Journal of Experimental Botany.
Oxford University Press, 2018. https://doi.org/10.1093/jxb/ery097.
ieee: T. R. Moturu et al., “Molecular evolution and diversification of the
SMXL gene family,” Journal of Experimental Botany, vol. 69, no. 9. Oxford
University Press, pp. 2367–2378, 2018.
ista: Moturu TR, Thula S, Singh RK, Nodzyński T, Vařeková RS, Friml J, Simon S.
2018. Molecular evolution and diversification of the SMXL gene family. Journal
of Experimental Botany. 69(9), 2367–2378.
mla: Moturu, Taraka Ramji, et al. “Molecular Evolution and Diversification of the
SMXL Gene Family.” Journal of Experimental Botany, vol. 69, no. 9, Oxford
University Press, 2018, pp. 2367–78, doi:10.1093/jxb/ery097.
short: T.R. Moturu, S. Thula, R.K. Singh, T. Nodzyński, R.S. Vařeková, J. Friml,
S. Simon, Journal of Experimental Botany 69 (2018) 2367–2378.
date_created: 2022-03-18T12:43:22Z
date_published: 2018-04-13T00:00:00Z
date_updated: 2025-05-07T11:12:33Z
day: '13'
department:
- _id: JiFr
doi: 10.1093/jxb/ery097
ec_funded: 1
external_id:
isi:
- '000430727000016'
pmid:
- '29538714'
intvolume: ' 69'
isi: 1
issue: '9'
keyword:
- Plant Science
- Physiology
language:
- iso: eng
month: '04'
oa_version: None
page: 2367-2378
pmid: 1
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: Journal of Experimental Botany
publication_identifier:
eissn:
- 1460-2431
issn:
- 0022-0957
publication_status: published
publisher: Oxford University Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: Molecular evolution and diversification of the SMXL gene family
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 69
year: '2018'
...
---
_id: '191'
abstract:
- lang: eng
text: Intercellular distribution of the plant hormone auxin largely depends on the
polar subcellular distribution of the plasma membrane PIN-FORMED (PIN) auxin transporters.
PIN polarity switches in response to different developmental and environmental
signals have been shown to redirect auxin fluxes mediating certain developmental
responses. PIN phosphorylation at different sites and by different kinases is
crucial for PIN function. Here we investigate the role of PIN phosphorylation
during gravitropic response. Loss- and gain-of-function mutants in PINOID and
related kinases but not in D6PK kinase as well as mutations mimicking constitutive
dephosphorylated or phosphorylated status of two clusters of predicted phosphorylation
sites partially disrupted PIN3 phosphorylation and caused defects in gravitropic
bending in roots and hypocotyls. In particular, they impacted PIN3 polarity rearrangements
in response to gravity and during feed-back regulation by auxin itself. Thus PIN
phosphorylation, besides regulating transport activity and apical-basal targeting,
is also important for the rapid polarity switches in response to environmental
and endogenous signals.
article_number: '10279'
article_processing_charge: No
author:
- first_name: Peter
full_name: Grones, Peter
id: 399876EC-F248-11E8-B48F-1D18A9856A87
last_name: Grones
- first_name: Melinda F
full_name: Abas, Melinda F
id: 3CFB3B1C-F248-11E8-B48F-1D18A9856A87
last_name: Abas
- first_name: Jakub
full_name: Hajny, Jakub
id: 4800CC20-F248-11E8-B48F-1D18A9856A87
last_name: Hajny
orcid: 0000-0003-2140-7195
- first_name: Angharad
full_name: Jones, Angharad
last_name: Jones
- first_name: Sascha
full_name: Waidmann, Sascha
last_name: Waidmann
- first_name: Jürgen
full_name: Kleine Vehn, Jürgen
last_name: Kleine Vehn
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Grones P, Abas MF, Hajny J, et al. PID/WAG-mediated phosphorylation of the
Arabidopsis PIN3 auxin transporter mediates polarity switches during gravitropism.
Scientific Reports. 2018;8(1). doi:10.1038/s41598-018-28188-1
apa: Grones, P., Abas, M. F., Hajny, J., Jones, A., Waidmann, S., Kleine Vehn, J.,
& Friml, J. (2018). PID/WAG-mediated phosphorylation of the Arabidopsis PIN3
auxin transporter mediates polarity switches during gravitropism. Scientific
Reports. Springer. https://doi.org/10.1038/s41598-018-28188-1
chicago: Grones, Peter, Melinda F Abas, Jakub Hajny, Angharad Jones, Sascha Waidmann,
Jürgen Kleine Vehn, and Jiří Friml. “PID/WAG-Mediated Phosphorylation of the Arabidopsis
PIN3 Auxin Transporter Mediates Polarity Switches during Gravitropism.” Scientific
Reports. Springer, 2018. https://doi.org/10.1038/s41598-018-28188-1.
ieee: P. Grones et al., “PID/WAG-mediated phosphorylation of the Arabidopsis
PIN3 auxin transporter mediates polarity switches during gravitropism,” Scientific
Reports, vol. 8, no. 1. Springer, 2018.
ista: Grones P, Abas MF, Hajny J, Jones A, Waidmann S, Kleine Vehn J, Friml J. 2018.
PID/WAG-mediated phosphorylation of the Arabidopsis PIN3 auxin transporter mediates
polarity switches during gravitropism. Scientific Reports. 8(1), 10279.
mla: Grones, Peter, et al. “PID/WAG-Mediated Phosphorylation of the Arabidopsis
PIN3 Auxin Transporter Mediates Polarity Switches during Gravitropism.” Scientific
Reports, vol. 8, no. 1, 10279, Springer, 2018, doi:10.1038/s41598-018-28188-1.
short: P. Grones, M.F. Abas, J. Hajny, A. Jones, S. Waidmann, J. Kleine Vehn, J.
Friml, Scientific Reports 8 (2018).
date_created: 2018-12-11T11:45:06Z
date_published: 2018-07-06T00:00:00Z
date_updated: 2025-05-07T11:12:31Z
day: '06'
ddc:
- '581'
department:
- _id: JiFr
- _id: EvBe
doi: 10.1038/s41598-018-28188-1
ec_funded: 1
external_id:
isi:
- '000437673200053'
file:
- access_level: open_access
checksum: 266b03f4fb8198e83141617aaa99dcab
content_type: application/pdf
creator: dernst
date_created: 2018-12-17T15:38:56Z
date_updated: 2020-07-14T12:45:20Z
file_id: '5714'
file_name: 2018_ScientificReports_Grones.pdf
file_size: 2413876
relation: main_file
file_date_updated: 2020-07-14T12:45:20Z
has_accepted_license: '1'
intvolume: ' 8'
isi: 1
issue: '1'
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Scientific Reports
publication_status: published
publisher: Springer
publist_id: '7729'
quality_controlled: '1'
related_material:
record:
- id: '8822'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: PID/WAG-mediated phosphorylation of the Arabidopsis PIN3 auxin transporter
mediates polarity switches during gravitropism
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 8
year: '2018'
...
---
_id: '192'
abstract:
- lang: eng
text: The phytohormone auxin is the information carrier in a plethora of developmental
and physiological processes in plants(1). It has been firmly established that
canonical, nuclear auxin signalling acts through regulation of gene transcription(2).
Here, we combined microfluidics, live imaging, genetic engineering and computational
modelling to reanalyse the classical case of root growth inhibition(3) by auxin.
We show that Arabidopsis roots react to addition and removal of auxin by extremely
rapid adaptation of growth rate. This process requires intracellular auxin perception
but not transcriptional reprogramming. The formation of the canonical TIR1/AFB-Aux/IAA
co-receptor complex is required for the growth regulation, hinting to a novel,
non-transcriptional branch of this signalling pathway. Our results challenge the
current understanding of root growth regulation by auxin and suggest another,
presumably non-transcriptional, signalling output of the canonical auxin pathway.
article_processing_charge: No
article_type: original
author:
- first_name: Matyas
full_name: Fendrych, Matyas
id: 43905548-F248-11E8-B48F-1D18A9856A87
last_name: Fendrych
orcid: 0000-0002-9767-8699
- first_name: Maria
full_name: Akhmanova, Maria
id: 3425EC26-F248-11E8-B48F-1D18A9856A87
last_name: Akhmanova
orcid: 0000-0003-1522-3162
- first_name: Jack
full_name: Merrin, Jack
id: 4515C308-F248-11E8-B48F-1D18A9856A87
last_name: Merrin
orcid: 0000-0001-5145-4609
- first_name: Matous
full_name: Glanc, Matous
last_name: Glanc
- first_name: Shinya
full_name: Hagihara, Shinya
last_name: Hagihara
- first_name: Koji
full_name: Takahashi, Koji
last_name: Takahashi
- first_name: Naoyuki
full_name: Uchida, Naoyuki
last_name: Uchida
- first_name: Keiko U
full_name: Torii, Keiko U
last_name: Torii
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Fendrych M, Akhmanova M, Merrin J, et al. Rapid and reversible root growth
inhibition by TIR1 auxin signalling. Nature Plants. 2018;4(7):453-459.
doi:10.1038/s41477-018-0190-1
apa: Fendrych, M., Akhmanova, M., Merrin, J., Glanc, M., Hagihara, S., Takahashi,
K., … Friml, J. (2018). Rapid and reversible root growth inhibition by TIR1 auxin
signalling. Nature Plants. Springer Nature. https://doi.org/10.1038/s41477-018-0190-1
chicago: Fendrych, Matyas, Maria Akhmanova, Jack Merrin, Matous Glanc, Shinya Hagihara,
Koji Takahashi, Naoyuki Uchida, Keiko U Torii, and Jiří Friml. “Rapid and Reversible
Root Growth Inhibition by TIR1 Auxin Signalling.” Nature Plants. Springer
Nature, 2018. https://doi.org/10.1038/s41477-018-0190-1.
ieee: M. Fendrych et al., “Rapid and reversible root growth inhibition by
TIR1 auxin signalling,” Nature Plants, vol. 4, no. 7. Springer Nature,
pp. 453–459, 2018.
ista: Fendrych M, Akhmanova M, Merrin J, Glanc M, Hagihara S, Takahashi K, Uchida
N, Torii KU, Friml J. 2018. Rapid and reversible root growth inhibition by TIR1
auxin signalling. Nature Plants. 4(7), 453–459.
mla: Fendrych, Matyas, et al. “Rapid and Reversible Root Growth Inhibition by TIR1
Auxin Signalling.” Nature Plants, vol. 4, no. 7, Springer Nature, 2018,
pp. 453–59, doi:10.1038/s41477-018-0190-1.
short: M. Fendrych, M. Akhmanova, J. Merrin, M. Glanc, S. Hagihara, K. Takahashi,
N. Uchida, K.U. Torii, J. Friml, Nature Plants 4 (2018) 453–459.
date_created: 2018-12-11T11:45:07Z
date_published: 2018-06-25T00:00:00Z
date_updated: 2023-09-15T12:11:03Z
day: '25'
department:
- _id: JiFr
- _id: DaSi
- _id: NanoFab
doi: 10.1038/s41477-018-0190-1
external_id:
isi:
- '000443221200017'
pmid:
- '29942048'
intvolume: ' 4'
isi: 1
issue: '7'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pubmed/29942048
month: '06'
oa: 1
oa_version: Submitted Version
page: 453 - 459
pmid: 1
publication: Nature Plants
publication_status: published
publisher: Springer Nature
publist_id: '7728'
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/new-mechanism-for-the-plant-hormone-auxin-discovered/
scopus_import: '1'
status: public
title: Rapid and reversible root growth inhibition by TIR1 auxin signalling
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 4
year: '2018'
...
---
_id: '203'
abstract:
- lang: eng
text: Asymmetric auxin distribution is instrumental for the differential growth
that causes organ bending on tropic stimuli and curvatures during plant development.
Local differences in auxin concentrations are achieved mainly by polarized cellular
distribution of PIN auxin transporters, but whether other mechanisms involving
auxin homeostasis are also relevant for the formation of auxin gradients is not
clear. Here we show that auxin methylation is required for asymmetric auxin distribution
across the hypocotyl, particularly during its response to gravity. We found that
loss-of-function mutants in Arabidopsis IAA CARBOXYL METHYLTRANSFERASE1 (IAMT1)
prematurely unfold the apical hook, and that their hypocotyls are impaired in
gravitropic reorientation. This defect is linked to an auxin-dependent increase
in PIN gene expression, leading to an increased polar auxin transport and lack
of asymmetric distribution of PIN3 in the iamt1 mutant. Gravitropic reorientation
in the iamt1 mutant could be restored with either endodermis-specific expression
of IAMT1 or partial inhibition of polar auxin transport, which also results in
normal PIN gene expression levels. We propose that IAA methylation is necessary
in gravity-sensing cells to restrict polar auxin transport within the range of
auxin levels that allow for differential responses.
article_processing_charge: No
author:
- first_name: Mohamad
full_name: Abbas, Mohamad
id: 47E8FC1C-F248-11E8-B48F-1D18A9856A87
last_name: Abbas
- first_name: García J
full_name: Hernández, García J
last_name: Hernández
- first_name: Stephan
full_name: Pollmann, Stephan
last_name: Pollmann
- first_name: Sophia L
full_name: Samodelov, Sophia L
last_name: Samodelov
- first_name: Martina
full_name: Kolb, Martina
last_name: Kolb
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Ulrich Z
full_name: Hammes, Ulrich Z
last_name: Hammes
- first_name: Matias D
full_name: Zurbriggen, Matias D
last_name: Zurbriggen
- first_name: Miguel
full_name: Blázquez, Miguel
last_name: Blázquez
- first_name: David
full_name: Alabadí, David
last_name: Alabadí
citation:
ama: Abbas M, Hernández GJ, Pollmann S, et al. Auxin methylation is required for
differential growth in Arabidopsis. PNAS. 2018;115(26):6864-6869. doi:10.1073/pnas.1806565115
apa: Abbas, M., Hernández, G. J., Pollmann, S., Samodelov, S. L., Kolb, M., Friml,
J., … Alabadí, D. (2018). Auxin methylation is required for differential growth
in Arabidopsis. PNAS. National Academy of Sciences. https://doi.org/10.1073/pnas.1806565115
chicago: Abbas, Mohamad, García J Hernández, Stephan Pollmann, Sophia L Samodelov,
Martina Kolb, Jiří Friml, Ulrich Z Hammes, Matias D Zurbriggen, Miguel Blázquez,
and David Alabadí. “Auxin Methylation Is Required for Differential Growth in Arabidopsis.”
PNAS. National Academy of Sciences, 2018. https://doi.org/10.1073/pnas.1806565115.
ieee: M. Abbas et al., “Auxin methylation is required for differential growth
in Arabidopsis,” PNAS, vol. 115, no. 26. National Academy of Sciences,
pp. 6864–6869, 2018.
ista: Abbas M, Hernández GJ, Pollmann S, Samodelov SL, Kolb M, Friml J, Hammes UZ,
Zurbriggen MD, Blázquez M, Alabadí D. 2018. Auxin methylation is required for
differential growth in Arabidopsis. PNAS. 115(26), 6864–6869.
mla: Abbas, Mohamad, et al. “Auxin Methylation Is Required for Differential Growth
in Arabidopsis.” PNAS, vol. 115, no. 26, National Academy of Sciences,
2018, pp. 6864–69, doi:10.1073/pnas.1806565115.
short: M. Abbas, G.J. Hernández, S. Pollmann, S.L. Samodelov, M. Kolb, J. Friml,
U.Z. Hammes, M.D. Zurbriggen, M. Blázquez, D. Alabadí, PNAS 115 (2018) 6864–6869.
date_created: 2018-12-11T11:45:11Z
date_published: 2018-06-26T00:00:00Z
date_updated: 2025-05-07T11:12:32Z
day: '26'
department:
- _id: JiFr
doi: 10.1073/pnas.1806565115
ec_funded: 1
external_id:
isi:
- '000436245000096'
intvolume: ' 115'
isi: 1
issue: '26'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://eprints.nottingham.ac.uk/52388/
month: '06'
oa: 1
oa_version: None
page: 6864-6869
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: PNAS
publication_status: published
publisher: National Academy of Sciences
publist_id: '7710'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Auxin methylation is required for differential growth in Arabidopsis
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 115
year: '2018'
...
---
_id: '462'
abstract:
- lang: eng
text: 'AtNHX5 and AtNHX6 are endosomal Na+,K+/H+ antiporters that are critical for
growth and development in Arabidopsis, but the mechanism behind their action remains
unknown. Here, we report that AtNHX5 and AtNHX6, functioning as H+ leak, control
auxin homeostasis and auxin-mediated development. We found that nhx5 nhx6 exhibited
growth variations of auxin-related defects. We further showed that nhx5 nhx6 was
affected in auxin homeostasis. Genetic analysis showed that AtNHX5 and AtNHX6
were required for the function of the ER-localized auxin transporter PIN5. Although
AtNHX5 and AtNHX6 were co-localized with PIN5 at ER, they did not interact directly.
Instead, the conserved acidic residues in AtNHX5 and AtNHX6, which are essential
for exchange activity, were required for PIN5 function. AtNHX5 and AtNHX6 regulated
the pH in ER. Overall, AtNHX5 and AtNHX6 may regulate auxin transport across the
ER via the pH gradient created by their transport activity. H+-leak pathway provides
a fine-tuning mechanism that controls cellular auxin fluxes. '
acknowledgement: 'This work was supported by the National Natural Science Foundation
of China (31571464, 31371438 and 31070222 to Q.S.Q.), the National Basic Research
Program of China (973 project, 2013CB429904 to Q.S.Q.), the Research Fund for the
Doctoral Program of Higher Education of China (20130211110001 to Q.S.Q.), the Ministry
of Education, Youth and Sports of the Czech Republic (the National Program for Sustainability
I, LO1204), and The Czech Science Foundation GAČR (GA13–40637S) to JF. We thank
Dr. Tom J. Guilfoyle for DR5::GUS line and Dr. Jia Li for pBIB‐RFP vector and DR5::GFP
line. We thank Liping Guan and Yang Zhao for their help with the confocal microscope
assay. '
article_processing_charge: No
article_type: original
author:
- first_name: Ligang
full_name: Fan, Ligang
last_name: Fan
- first_name: Lei
full_name: Zhao, Lei
last_name: Zhao
- first_name: Wei
full_name: Hu, Wei
last_name: Hu
- first_name: Weina
full_name: Li, Weina
last_name: Li
- first_name: Ondřej
full_name: Novák, Ondřej
last_name: Novák
- first_name: Miroslav
full_name: Strnad, Miroslav
last_name: Strnad
- first_name: Sibu
full_name: Simon, Sibu
id: 4542EF9A-F248-11E8-B48F-1D18A9856A87
last_name: Simon
orcid: 0000-0002-1998-6741
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Jinbo
full_name: Shen, Jinbo
last_name: Shen
- first_name: Liwen
full_name: Jiang, Liwen
last_name: Jiang
- first_name: Quan
full_name: Qiu, Quan
last_name: Qiu
citation:
ama: Fan L, Zhao L, Hu W, et al. NHX antiporters regulate the pH of endoplasmic
reticulum and auxin-mediated development. Plant, Cell and Environment.
2018;41:850-864. doi:10.1111/pce.13153
apa: Fan, L., Zhao, L., Hu, W., Li, W., Novák, O., Strnad, M., … Qiu, Q. (2018).
NHX antiporters regulate the pH of endoplasmic reticulum and auxin-mediated development.
Plant, Cell and Environment. Wiley-Blackwell. https://doi.org/10.1111/pce.13153
chicago: Fan, Ligang, Lei Zhao, Wei Hu, Weina Li, Ondřej Novák, Miroslav Strnad,
Sibu Simon, et al. “NHX Antiporters Regulate the PH of Endoplasmic Reticulum and
Auxin-Mediated Development.” Plant, Cell and Environment. Wiley-Blackwell,
2018. https://doi.org/10.1111/pce.13153.
ieee: L. Fan et al., “NHX antiporters regulate the pH of endoplasmic reticulum
and auxin-mediated development,” Plant, Cell and Environment, vol. 41.
Wiley-Blackwell, pp. 850–864, 2018.
ista: Fan L, Zhao L, Hu W, Li W, Novák O, Strnad M, Simon S, Friml J, Shen J, Jiang
L, Qiu Q. 2018. NHX antiporters regulate the pH of endoplasmic reticulum and auxin-mediated
development. Plant, Cell and Environment. 41, 850–864.
mla: Fan, Ligang, et al. “NHX Antiporters Regulate the PH of Endoplasmic Reticulum
and Auxin-Mediated Development.” Plant, Cell and Environment, vol. 41,
Wiley-Blackwell, 2018, pp. 850–64, doi:10.1111/pce.13153.
short: L. Fan, L. Zhao, W. Hu, W. Li, O. Novák, M. Strnad, S. Simon, J. Friml, J.
Shen, L. Jiang, Q. Qiu, Plant, Cell and Environment 41 (2018) 850–864.
date_created: 2018-12-11T11:46:36Z
date_published: 2018-05-01T00:00:00Z
date_updated: 2023-09-13T09:03:18Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1111/pce.13153
external_id:
isi:
- '000426870500012'
pmid:
- '29360148'
file:
- access_level: open_access
checksum: 6a20f843565f962cb20281cdf5e40914
content_type: application/pdf
creator: dernst
date_created: 2019-11-18T16:22:22Z
date_updated: 2020-07-14T12:46:32Z
file_id: '7042'
file_name: 2018_PlantCellEnv_Fan.pdf
file_size: 1937976
relation: main_file
file_date_updated: 2020-07-14T12:46:32Z
has_accepted_license: '1'
intvolume: ' 41'
isi: 1
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc/4.0/
month: '05'
oa: 1
oa_version: Submitted Version
page: 850 - 864
pmid: 1
publication: Plant, Cell and Environment
publication_status: published
publisher: Wiley-Blackwell
publist_id: '7359'
quality_controlled: '1'
scopus_import: '1'
status: public
title: NHX antiporters regulate the pH of endoplasmic reticulum and auxin-mediated
development
tmp:
image: /images/cc_by_nc.png
legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode
name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
short: CC BY-NC (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 41
year: '2018'
...
---
_id: '5673'
abstract:
- lang: eng
text: Cell polarity, manifested by the localization of proteins to distinct polar
plasma membrane domains, is a key prerequisite of multicellular life. In plants,
PIN auxin transporters are prominent polarity markers crucial for a plethora of
developmental processes. Cell polarity mechanisms in plants are distinct from
other eukaryotes and still largely elusive. In particular, how the cell polarities
are propagated and maintained following cell division remains unknown. Plant cytokinesis
is orchestrated by the cell plate—a transient centrifugally growing endomembrane
compartment ultimately forming the cross wall1. Trafficking of polar membrane
proteins is typically redirected to the cell plate, and these will consequently
have opposite polarity in at least one of the daughter cells2–5. Here, we provide
mechanistic insights into post-cytokinetic re-establishment of cell polarity as
manifested by the apical, polar localization of PIN2. We show that the apical
domain is defined in a cell-intrinsic manner and that re-establishment of PIN2
localization to this domain requires de novo protein secretion and endocytosis,
but not basal-to-apical transcytosis. Furthermore, we identify a PINOID-related
kinase WAG1, which phosphorylates PIN2 in vitro6 and is transcriptionally upregulated
specifically in dividing cells, as a crucial regulator of post-cytokinetic PIN2
polarity re-establishment.
article_processing_charge: No
author:
- first_name: Matous
full_name: Glanc, Matous
id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
last_name: Glanc
orcid: 0000-0003-0619-7783
- first_name: Matyas
full_name: Fendrych, Matyas
id: 43905548-F248-11E8-B48F-1D18A9856A87
last_name: Fendrych
orcid: 0000-0002-9767-8699
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Glanc M, Fendrych M, Friml J. Mechanistic framework for cell-intrinsic re-establishment
of PIN2 polarity after cell division. Nature Plants. 2018;4(12):1082-1088.
doi:10.1038/s41477-018-0318-3
apa: Glanc, M., Fendrych, M., & Friml, J. (2018). Mechanistic framework for
cell-intrinsic re-establishment of PIN2 polarity after cell division. Nature
Plants. Nature Research. https://doi.org/10.1038/s41477-018-0318-3
chicago: Glanc, Matous, Matyas Fendrych, and Jiří Friml. “Mechanistic Framework
for Cell-Intrinsic Re-Establishment of PIN2 Polarity after Cell Division.” Nature
Plants. Nature Research, 2018. https://doi.org/10.1038/s41477-018-0318-3.
ieee: M. Glanc, M. Fendrych, and J. Friml, “Mechanistic framework for cell-intrinsic
re-establishment of PIN2 polarity after cell division,” Nature Plants,
vol. 4, no. 12. Nature Research, pp. 1082–1088, 2018.
ista: Glanc M, Fendrych M, Friml J. 2018. Mechanistic framework for cell-intrinsic
re-establishment of PIN2 polarity after cell division. Nature Plants. 4(12), 1082–1088.
mla: Glanc, Matous, et al. “Mechanistic Framework for Cell-Intrinsic Re-Establishment
of PIN2 Polarity after Cell Division.” Nature Plants, vol. 4, no. 12, Nature
Research, 2018, pp. 1082–88, doi:10.1038/s41477-018-0318-3.
short: M. Glanc, M. Fendrych, J. Friml, Nature Plants 4 (2018) 1082–1088.
date_created: 2018-12-16T22:59:18Z
date_published: 2018-12-03T00:00:00Z
date_updated: 2023-10-17T12:19:28Z
day: '03'
department:
- _id: JiFr
doi: 10.1038/s41477-018-0318-3
ec_funded: 1
external_id:
isi:
- '000454576600017'
pmid:
- '30518833'
intvolume: ' 4'
isi: 1
issue: '12'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pubmed/30518833
month: '12'
oa: 1
oa_version: Submitted Version
page: 1082-1088
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Nature Plants
publication_identifier:
issn:
- 2055-0278
publication_status: published
publisher: Nature Research
quality_controlled: '1'
scopus_import: '1'
status: public
title: Mechanistic framework for cell-intrinsic re-establishment of PIN2 polarity
after cell division
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 4
year: '2018'
...
---
_id: '5830'
abstract:
- lang: eng
text: CLE peptides have been implicated in various developmental processes of plants
and mediate their responses to environmental stimuli. However, the biological
relevance of most CLE genes remains to be functionally characterized. Here, we
report that CLE9, which is expressed in stomata, acts as an essential regulator
in the induction of stomatal closure. Exogenous application of CLE9 peptides or
overexpression of CLE9 effectively led to stomatal closure and enhanced drought
tolerance, whereas CLE9 loss-of-function mutants were sensitivity to drought stress.
CLE9-induced stomatal closure was impaired in abscisic acid (ABA)-deficient mutants,
indicating that ABA is required for CLE9-medaited guard cell signalling. We further
deciphered that two guard cell ABA-signalling components, OST1 and SLAC1, were
responsible for CLE9-induced stomatal closure. MPK3 and MPK6 were activated by
the CLE9 peptide, and CLE9 peptides failed to close stomata in mpk3 and mpk6 mutants.
In addition, CLE9 peptides stimulated the induction of hydrogen peroxide (H2O2)
and nitric oxide (NO) synthesis associated with stomatal closure, which was abolished
in the NADPH oxidase-deficient mutants or nitric reductase mutants, respectively.
Collectively, our results reveal a novel ABA-dependent function of CLE9 in the
regulation of stomatal apertures, thereby suggesting a potential role of CLE9
in the stress acclimatization of plants.
article_processing_charge: No
author:
- first_name: Luosha
full_name: Zhang, Luosha
last_name: Zhang
- first_name: Xiong
full_name: Shi, Xiong
last_name: Shi
- first_name: Yutao
full_name: Zhang, Yutao
last_name: Zhang
- first_name: Jiajing
full_name: Wang, Jiajing
last_name: Wang
- first_name: Jingwei
full_name: Yang, Jingwei
last_name: Yang
- first_name: Takashi
full_name: Ishida, Takashi
last_name: Ishida
- first_name: Wenqian
full_name: Jiang, Wenqian
last_name: Jiang
- first_name: Xiangyu
full_name: Han, Xiangyu
last_name: Han
- first_name: Jingke
full_name: Kang, Jingke
last_name: Kang
- first_name: Xuening
full_name: Wang, Xuening
last_name: Wang
- first_name: Lixia
full_name: Pan, Lixia
last_name: Pan
- first_name: Shuo
full_name: Lv, Shuo
last_name: Lv
- first_name: Bing
full_name: Cao, Bing
last_name: Cao
- first_name: Yonghong
full_name: Zhang, Yonghong
last_name: Zhang
- first_name: Jinbin
full_name: Wu, Jinbin
last_name: Wu
- first_name: Huibin
full_name: Han, Huibin
id: 31435098-F248-11E8-B48F-1D18A9856A87
last_name: Han
- first_name: Zhubing
full_name: Hu, Zhubing
last_name: Hu
- first_name: Langjun
full_name: Cui, Langjun
last_name: Cui
- first_name: Shinichiro
full_name: Sawa, Shinichiro
last_name: Sawa
- first_name: Junmin
full_name: He, Junmin
last_name: He
- first_name: Guodong
full_name: Wang, Guodong
last_name: Wang
citation:
ama: Zhang L, Shi X, Zhang Y, et al. CLE9 peptide-induced stomatal closure is mediated
by abscisic acid, hydrogen peroxide, and nitric oxide in arabidopsis thaliana.
Plant Cell and Environment. 2018. doi:10.1111/pce.13475
apa: Zhang, L., Shi, X., Zhang, Y., Wang, J., Yang, J., Ishida, T., … Wang, G. (2018).
CLE9 peptide-induced stomatal closure is mediated by abscisic acid, hydrogen peroxide,
and nitric oxide in arabidopsis thaliana. Plant Cell and Environment. Wiley.
https://doi.org/10.1111/pce.13475
chicago: Zhang, Luosha, Xiong Shi, Yutao Zhang, Jiajing Wang, Jingwei Yang, Takashi
Ishida, Wenqian Jiang, et al. “CLE9 Peptide-Induced Stomatal Closure Is Mediated
by Abscisic Acid, Hydrogen Peroxide, and Nitric Oxide in Arabidopsis Thaliana.”
Plant Cell and Environment. Wiley, 2018. https://doi.org/10.1111/pce.13475.
ieee: L. Zhang et al., “CLE9 peptide-induced stomatal closure is mediated
by abscisic acid, hydrogen peroxide, and nitric oxide in arabidopsis thaliana,”
Plant Cell and Environment. Wiley, 2018.
ista: Zhang L, Shi X, Zhang Y, Wang J, Yang J, Ishida T, Jiang W, Han X, Kang J,
Wang X, Pan L, Lv S, Cao B, Zhang Y, Wu J, Han H, Hu Z, Cui L, Sawa S, He J, Wang
G. 2018. CLE9 peptide-induced stomatal closure is mediated by abscisic acid, hydrogen
peroxide, and nitric oxide in arabidopsis thaliana. Plant Cell and Environment.
mla: Zhang, Luosha, et al. “CLE9 Peptide-Induced Stomatal Closure Is Mediated by
Abscisic Acid, Hydrogen Peroxide, and Nitric Oxide in Arabidopsis Thaliana.” Plant
Cell and Environment, Wiley, 2018, doi:10.1111/pce.13475.
short: L. Zhang, X. Shi, Y. Zhang, J. Wang, J. Yang, T. Ishida, W. Jiang, X. Han,
J. Kang, X. Wang, L. Pan, S. Lv, B. Cao, Y. Zhang, J. Wu, H. Han, Z. Hu, L. Cui,
S. Sawa, J. He, G. Wang, Plant Cell and Environment (2018).
date_created: 2019-01-13T22:59:11Z
date_published: 2018-10-31T00:00:00Z
date_updated: 2023-09-11T12:43:31Z
day: '31'
department:
- _id: JiFr
doi: 10.1111/pce.13475
external_id:
isi:
- '000459014800021'
pmid:
- '30378140'
isi: 1
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pubmed/30378140
month: '10'
oa: 1
oa_version: Published Version
pmid: 1
publication: Plant Cell and Environment
publication_identifier:
issn:
- '01407791'
publication_status: epub_ahead
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: CLE9 peptide-induced stomatal closure is mediated by abscisic acid, hydrogen
peroxide, and nitric oxide in arabidopsis thaliana
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2018'
...
---
_id: '146'
abstract:
- lang: eng
text: The root cap protects the stem cell niche of angiosperm roots from damage.
In Arabidopsis, lateral root cap (LRC) cells covering the meristematic zone are
regularly lost through programmed cell death, while the outermost layer of the
root cap covering the tip is repeatedly sloughed. Efficient coordination with
stem cells producing new layers is needed to maintain a constant size of the cap.
We present a signalling pair, the peptide IDA-LIKE1 (IDL1) and its receptor HAESA-LIKE2
(HSL2), mediating such communication. Live imaging over several days characterized
this process from initial fractures in LRC cell files to full separation of a
layer. Enhanced expression of IDL1 in the separating root cap layers resulted
in increased frequency of sloughing, balanced with generation of new layers in
a HSL2-dependent manner. Transcriptome analyses linked IDL1-HSL2 signalling to
the transcription factors BEARSKIN1/2 and genes associated with programmed cell
death. Mutations in either IDL1 or HSL2 slowed down cell division, maturation
and separation. Thus, IDL1-HSL2 signalling potentiates dynamic regulation of the
homeostatic balance between stem cell division and sloughing activity.
article_processing_charge: No
article_type: original
author:
- first_name: Chun Lin
full_name: Shi, Chun Lin
last_name: Shi
- first_name: Daniel
full_name: Von Wangenheim, Daniel
id: 49E91952-F248-11E8-B48F-1D18A9856A87
last_name: Von Wangenheim
orcid: 0000-0002-6862-1247
- first_name: Ullrich
full_name: Herrmann, Ullrich
last_name: Herrmann
- first_name: Mari
full_name: Wildhagen, Mari
last_name: Wildhagen
- first_name: Ivan
full_name: Kulik, Ivan
id: F0AB3FCE-02D1-11E9-BD0E-99399A5D3DEB
last_name: Kulik
- first_name: Andreas
full_name: Kopf, Andreas
last_name: Kopf
- first_name: Takashi
full_name: Ishida, Takashi
last_name: Ishida
- first_name: Vilde
full_name: Olsson, Vilde
last_name: Olsson
- first_name: Mari Kristine
full_name: Anker, Mari Kristine
last_name: Anker
- first_name: Markus
full_name: Albert, Markus
last_name: Albert
- first_name: Melinka A
full_name: Butenko, Melinka A
last_name: Butenko
- first_name: Georg
full_name: Felix, Georg
last_name: Felix
- first_name: Shinichiro
full_name: Sawa, Shinichiro
last_name: Sawa
- first_name: Manfred
full_name: Claassen, Manfred
last_name: Claassen
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Reidunn B
full_name: Aalen, Reidunn B
last_name: Aalen
citation:
ama: Shi CL, von Wangenheim D, Herrmann U, et al. The dynamics of root cap sloughing
in Arabidopsis is regulated by peptide signalling. Nature Plants. 2018;4(8):596-604.
doi:10.1038/s41477-018-0212-z
apa: Shi, C. L., von Wangenheim, D., Herrmann, U., Wildhagen, M., Kulik, I., Kopf,
A., … Aalen, R. B. (2018). The dynamics of root cap sloughing in Arabidopsis is
regulated by peptide signalling. Nature Plants. Nature Publishing Group.
https://doi.org/10.1038/s41477-018-0212-z
chicago: Shi, Chun Lin, Daniel von Wangenheim, Ullrich Herrmann, Mari Wildhagen,
Ivan Kulik, Andreas Kopf, Takashi Ishida, et al. “The Dynamics of Root Cap Sloughing
in Arabidopsis Is Regulated by Peptide Signalling.” Nature Plants. Nature
Publishing Group, 2018. https://doi.org/10.1038/s41477-018-0212-z.
ieee: C. L. Shi et al., “The dynamics of root cap sloughing in Arabidopsis
is regulated by peptide signalling,” Nature Plants, vol. 4, no. 8. Nature
Publishing Group, pp. 596–604, 2018.
ista: Shi CL, von Wangenheim D, Herrmann U, Wildhagen M, Kulik I, Kopf A, Ishida
T, Olsson V, Anker MK, Albert M, Butenko MA, Felix G, Sawa S, Claassen M, Friml
J, Aalen RB. 2018. The dynamics of root cap sloughing in Arabidopsis is regulated
by peptide signalling. Nature Plants. 4(8), 596–604.
mla: Shi, Chun Lin, et al. “The Dynamics of Root Cap Sloughing in Arabidopsis Is
Regulated by Peptide Signalling.” Nature Plants, vol. 4, no. 8, Nature
Publishing Group, 2018, pp. 596–604, doi:10.1038/s41477-018-0212-z.
short: C.L. Shi, D. von Wangenheim, U. Herrmann, M. Wildhagen, I. Kulik, A. Kopf,
T. Ishida, V. Olsson, M.K. Anker, M. Albert, M.A. Butenko, G. Felix, S. Sawa,
M. Claassen, J. Friml, R.B. Aalen, Nature Plants 4 (2018) 596–604.
date_created: 2018-12-11T11:44:52Z
date_published: 2018-07-30T00:00:00Z
date_updated: 2023-09-19T10:08:45Z
day: '30'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1038/s41477-018-0212-z
external_id:
isi:
- '000443861300016'
pmid:
- '30061750'
file:
- access_level: open_access
checksum: da33101c76ee1b2dc5ab28fd2ccba9d0
content_type: application/pdf
creator: dernst
date_created: 2019-11-18T16:24:07Z
date_updated: 2020-07-14T12:44:56Z
file_id: '7043'
file_name: 2018_NaturePlants_Shi.pdf
file_size: 226829
relation: main_file
file_date_updated: 2020-07-14T12:44:56Z
has_accepted_license: '1'
intvolume: ' 4'
isi: 1
issue: '8'
language:
- iso: eng
month: '07'
oa: 1
oa_version: Submitted Version
page: 596 - 604
pmid: 1
publication: Nature Plants
publication_status: published
publisher: Nature Publishing Group
publist_id: '7777'
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/new-process-in-root-development-discovered/
scopus_import: '1'
status: public
title: The dynamics of root cap sloughing in Arabidopsis is regulated by peptide signalling
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 4
year: '2018'
...
---
_id: '147'
abstract:
- lang: eng
text: The trafficking of subcellular cargos in eukaryotic cells crucially depends
on vesicle budding, a process mediated by ARF-GEFs (ADP-ribosylation factor guanine
nucleotide exchange factors). In plants, ARF-GEFs play essential roles in endocytosis,
vacuolar trafficking, recycling, secretion, and polar trafficking. Moreover, they
are important for plant development, mainly through controlling the polar subcellular
localization of PIN-FORMED (PIN) transporters of the plant hormone auxin. Here,
using a chemical genetics screen in Arabidopsis thaliana, we identified Endosidin
4 (ES4), an inhibitor of eukaryotic ARF-GEFs. ES4 acts similarly to and synergistically
with the established ARF-GEF inhibitor Brefeldin A and has broad effects on intracellular
trafficking, including endocytosis, exocytosis, and vacuolar targeting. Additionally,
Arabidopsis and yeast (Sacharomyces cerevisiae) mutants defective in ARF-GEF show
altered sensitivity to ES4. ES4 interferes with the activation-based membrane
association of the ARF1 GTPases, but not of their mutant variants that are activated
independently of ARF-GEF activity. Biochemical approaches and docking simulations
confirmed that ES4 specifically targets the SEC7 domain-containing ARF-GEFs. These
observations collectively identify ES4 as a chemical tool enabling the study of
ARF-GEF-mediated processes, including ARF-GEF-mediated plant development.
acknowledgement: We thank Gerd Jürgens, Sandra Richter, and Sheng Yang He for providing
antibodies; Maciek Adamowski, Fernando Aniento, Sebastian Bednarek, Nico Callewaert,
Matyás Fendrych, Elena Feraru, and Mugurel I. Feraru for helpful suggestions; Siamsa
Doyle for critical reading of the manuscript and helpful comments and suggestions;
and Stephanie Smith and Martine De Cock for help in editing and language corrections.
We acknowledge the core facility Cellular Imaging of CEITEC supported by the Czech-BioImaging
large RI project (LM2015062 funded by MEYS CR) for their support with obtaining
scientific data presented in this article. Plant Sciences Core Facility of CEITEC
Masaryk University is gratefully acknowledged for obtaining part of the scientific
data presented in this article. We acknowledge support from the Fondation pour la
Recherche Médicale and from the Institut National du Cancer (J.C.). The research
leading to these results was funded by the European Research Council under the European
Union's 7th Framework Program (FP7/2007-2013)/ERC grant agreement numbers 282300
and 742985 and the Czech Science Foundation GAČR (GA18-26981S; J.F.); Ministry of
Education, Youth, and Sports/MEYS of the Czech Republic under the Project CEITEC
2020 (LQ1601; T.N.); the China Science Council for a predoctoral fellowship (Q.L.);
a joint research project within the framework of cooperation between the Research
Foundation-Flanders and the Bulgarian Academy of Sciences (VS.025.13N; K.M. and
E.R.); Vetenskapsrådet and Vinnova (Verket för Innovationssystem; S.R.), Knut och
Alice Wallenbergs Stiftelse via “Shapesystem” Grant 2012.0050 (S.R.), Kempe stiftelserna
(P.G.), Tryggers CTS410 (P.G.).
article_processing_charge: No
article_type: original
author:
- first_name: Urszula
full_name: Kania, Urszula
id: 4AE5C486-F248-11E8-B48F-1D18A9856A87
last_name: Kania
- first_name: Tomasz
full_name: Nodzyński, Tomasz
last_name: Nodzyński
- first_name: Qing
full_name: Lu, Qing
last_name: Lu
- first_name: Glenn R
full_name: Hicks, Glenn R
last_name: Hicks
- first_name: Wim
full_name: Nerinckx, Wim
last_name: Nerinckx
- first_name: Kiril
full_name: Mishev, Kiril
last_name: Mishev
- first_name: Francois
full_name: Peurois, Francois
last_name: Peurois
- first_name: Jacqueline
full_name: Cherfils, Jacqueline
last_name: Cherfils
- first_name: Rycke Riet Maria
full_name: De, Rycke Riet Maria
last_name: De
- first_name: Peter
full_name: Grones, Peter
id: 399876EC-F248-11E8-B48F-1D18A9856A87
last_name: Grones
- first_name: Stéphanie
full_name: Robert, Stéphanie
last_name: Robert
- first_name: Eugenia
full_name: Russinova, Eugenia
last_name: Russinova
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Kania U, Nodzyński T, Lu Q, et al. The inhibitor Endosidin 4 targets SEC7 domain-type
ARF GTPase exchange factors and interferes with sub cellular trafficking in eukaryotes.
The Plant Cell. 2018;30(10):2553-2572. doi:10.1105/tpc.18.00127
apa: Kania, U., Nodzyński, T., Lu, Q., Hicks, G. R., Nerinckx, W., Mishev, K., …
Friml, J. (2018). The inhibitor Endosidin 4 targets SEC7 domain-type ARF GTPase
exchange factors and interferes with sub cellular trafficking in eukaryotes. The
Plant Cell. Oxford University Press. https://doi.org/10.1105/tpc.18.00127
chicago: Kania, Urszula, Tomasz Nodzyński, Qing Lu, Glenn R Hicks, Wim Nerinckx,
Kiril Mishev, Francois Peurois, et al. “The Inhibitor Endosidin 4 Targets SEC7
Domain-Type ARF GTPase Exchange Factors and Interferes with Sub Cellular Trafficking
in Eukaryotes.” The Plant Cell. Oxford University Press, 2018. https://doi.org/10.1105/tpc.18.00127.
ieee: U. Kania et al., “The inhibitor Endosidin 4 targets SEC7 domain-type
ARF GTPase exchange factors and interferes with sub cellular trafficking in eukaryotes,”
The Plant Cell, vol. 30, no. 10. Oxford University Press, pp. 2553–2572,
2018.
ista: Kania U, Nodzyński T, Lu Q, Hicks GR, Nerinckx W, Mishev K, Peurois F, Cherfils
J, De RRM, Grones P, Robert S, Russinova E, Friml J. 2018. The inhibitor Endosidin
4 targets SEC7 domain-type ARF GTPase exchange factors and interferes with sub
cellular trafficking in eukaryotes. The Plant Cell. 30(10), 2553–2572.
mla: Kania, Urszula, et al. “The Inhibitor Endosidin 4 Targets SEC7 Domain-Type
ARF GTPase Exchange Factors and Interferes with Sub Cellular Trafficking in Eukaryotes.”
The Plant Cell, vol. 30, no. 10, Oxford University Press, 2018, pp. 2553–72,
doi:10.1105/tpc.18.00127.
short: U. Kania, T. Nodzyński, Q. Lu, G.R. Hicks, W. Nerinckx, K. Mishev, F. Peurois,
J. Cherfils, R.R.M. De, P. Grones, S. Robert, E. Russinova, J. Friml, The Plant
Cell 30 (2018) 2553–2572.
date_created: 2018-12-11T11:44:52Z
date_published: 2018-11-12T00:00:00Z
date_updated: 2025-05-07T11:12:30Z
day: '12'
department:
- _id: JiFr
doi: 10.1105/tpc.18.00127
ec_funded: 1
external_id:
isi:
- '000450000500023'
pmid:
- '30018156'
intvolume: ' 30'
isi: 1
issue: '10'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1105/tpc.18.00127
month: '11'
oa: 1
oa_version: Published Version
page: 2553 - 2572
pmid: 1
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: The Plant Cell
publication_identifier:
issn:
- 1040-4651
publication_status: published
publisher: Oxford University Press
publist_id: '7776'
quality_controlled: '1'
scopus_import: '1'
status: public
title: The inhibitor Endosidin 4 targets SEC7 domain-type ARF GTPase exchange factors
and interferes with sub cellular trafficking in eukaryotes
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 30
year: '2018'
...
---
_id: '148'
abstract:
- lang: eng
text: 'Land plants evolved from charophytic algae, among which Charophyceae possess
the most complex body plans. We present the genome of Chara braunii; comparison
of the genome to those of land plants identified evolutionary novelties for plant
terrestrialization and land plant heritage genes. C. braunii employs unique xylan
synthases for cell wall biosynthesis, a phragmoplast (cell separation) mechanism
similar to that of land plants, and many phytohormones. C. braunii plastids are
controlled via land-plant-like retrograde signaling, and transcriptional regulation
is more elaborate than in other algae. The morphological complexity of this organism
may result from expanded gene families, with three cases of particular note: genes
effecting tolerance to reactive oxygen species (ROS), LysM receptor-like kinases,
and transcription factors (TFs). Transcriptomic analysis of sexual reproductive
structures reveals intricate control by TFs, activity of the ROS gene network,
and the ancestral use of plant-like storage and stress protection proteins in
the zygote.'
acknowledgement: In-Data-Review
article_processing_charge: No
author:
- first_name: Tomoaki
full_name: Nishiyama, Tomoaki
last_name: Nishiyama
- first_name: Hidetoshi
full_name: Sakayama, Hidetoshi
last_name: Sakayama
- first_name: Jan
full_name: De Vries, Jan
last_name: De Vries
- first_name: Henrik
full_name: Buschmann, Henrik
last_name: Buschmann
- first_name: Denis
full_name: Saint Marcoux, Denis
last_name: Saint Marcoux
- first_name: Kristian
full_name: Ullrich, Kristian
last_name: Ullrich
- first_name: Fabian
full_name: Haas, Fabian
last_name: Haas
- first_name: Lisa
full_name: Vanderstraeten, Lisa
last_name: Vanderstraeten
- first_name: Dirk
full_name: Becker, Dirk
last_name: Becker
- first_name: Daniel
full_name: Lang, Daniel
last_name: Lang
- first_name: Stanislav
full_name: Vosolsobě, Stanislav
last_name: Vosolsobě
- first_name: Stephane
full_name: Rombauts, Stephane
last_name: Rombauts
- first_name: Per
full_name: Wilhelmsson, Per
last_name: Wilhelmsson
- first_name: Philipp
full_name: Janitza, Philipp
last_name: Janitza
- first_name: Ramona
full_name: Kern, Ramona
last_name: Kern
- first_name: Alexander
full_name: Heyl, Alexander
last_name: Heyl
- first_name: Florian
full_name: Rümpler, Florian
last_name: Rümpler
- first_name: Luz
full_name: Calderón Villalobos, Luz
last_name: Calderón Villalobos
- first_name: John
full_name: Clay, John
last_name: Clay
- first_name: Roman
full_name: Skokan, Roman
last_name: Skokan
- first_name: Atsushi
full_name: Toyoda, Atsushi
last_name: Toyoda
- first_name: Yutaka
full_name: Suzuki, Yutaka
last_name: Suzuki
- first_name: Hiroshi
full_name: Kagoshima, Hiroshi
last_name: Kagoshima
- first_name: Elio
full_name: Schijlen, Elio
last_name: Schijlen
- first_name: Navindra
full_name: Tajeshwar, Navindra
last_name: Tajeshwar
- first_name: Bruno
full_name: Catarino, Bruno
last_name: Catarino
- first_name: Alexander
full_name: Hetherington, Alexander
last_name: Hetherington
- first_name: Assia
full_name: Saltykova, Assia
last_name: Saltykova
- first_name: Clemence
full_name: Bonnot, Clemence
last_name: Bonnot
- first_name: Holger
full_name: Breuninger, Holger
last_name: Breuninger
- first_name: Aikaterini
full_name: Symeonidi, Aikaterini
last_name: Symeonidi
- first_name: Guru
full_name: Radhakrishnan, Guru
last_name: Radhakrishnan
- first_name: Filip
full_name: Van Nieuwerburgh, Filip
last_name: Van Nieuwerburgh
- first_name: Dieter
full_name: Deforce, Dieter
last_name: Deforce
- first_name: Caren
full_name: Chang, Caren
last_name: Chang
- first_name: Kenneth
full_name: Karol, Kenneth
last_name: Karol
- first_name: Rainer
full_name: Hedrich, Rainer
last_name: Hedrich
- first_name: Peter
full_name: Ulvskov, Peter
last_name: Ulvskov
- first_name: Gernot
full_name: Glöckner, Gernot
last_name: Glöckner
- first_name: Charles
full_name: Delwiche, Charles
last_name: Delwiche
- first_name: Jan
full_name: Petrášek, Jan
last_name: Petrášek
- first_name: Yves
full_name: Van De Peer, Yves
last_name: Van De Peer
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Mary
full_name: Beilby, Mary
last_name: Beilby
- first_name: Liam
full_name: Dolan, Liam
last_name: Dolan
- first_name: Yuji
full_name: Kohara, Yuji
last_name: Kohara
- first_name: Sumio
full_name: Sugano, Sumio
last_name: Sugano
- first_name: Asao
full_name: Fujiyama, Asao
last_name: Fujiyama
- first_name: Pierre Marc
full_name: Delaux, Pierre Marc
last_name: Delaux
- first_name: Marcel
full_name: Quint, Marcel
last_name: Quint
- first_name: Gunter
full_name: Theissen, Gunter
last_name: Theissen
- first_name: Martin
full_name: Hagemann, Martin
last_name: Hagemann
- first_name: Jesper
full_name: Harholt, Jesper
last_name: Harholt
- first_name: Christophe
full_name: Dunand, Christophe
last_name: Dunand
- first_name: Sabine
full_name: Zachgo, Sabine
last_name: Zachgo
- first_name: Jane
full_name: Langdale, Jane
last_name: Langdale
- first_name: Florian
full_name: Maumus, Florian
last_name: Maumus
- first_name: Dominique
full_name: Van Der Straeten, Dominique
last_name: Van Der Straeten
- first_name: Sven B
full_name: Gould, Sven B
last_name: Gould
- first_name: Stefan
full_name: Rensing, Stefan
last_name: Rensing
citation:
ama: 'Nishiyama T, Sakayama H, De Vries J, et al. The Chara genome: Secondary complexity
and implications for plant terrestrialization. Cell. 2018;174(2):448-464.e24.
doi:10.1016/j.cell.2018.06.033'
apa: 'Nishiyama, T., Sakayama, H., De Vries, J., Buschmann, H., Saint Marcoux, D.,
Ullrich, K., … Rensing, S. (2018). The Chara genome: Secondary complexity and
implications for plant terrestrialization. Cell. Cell Press. https://doi.org/10.1016/j.cell.2018.06.033'
chicago: 'Nishiyama, Tomoaki, Hidetoshi Sakayama, Jan De Vries, Henrik Buschmann,
Denis Saint Marcoux, Kristian Ullrich, Fabian Haas, et al. “The Chara Genome:
Secondary Complexity and Implications for Plant Terrestrialization.” Cell.
Cell Press, 2018. https://doi.org/10.1016/j.cell.2018.06.033.'
ieee: 'T. Nishiyama et al., “The Chara genome: Secondary complexity and implications
for plant terrestrialization,” Cell, vol. 174, no. 2. Cell Press, p. 448–464.e24,
2018.'
ista: 'Nishiyama T, Sakayama H, De Vries J, Buschmann H, Saint Marcoux D, Ullrich
K, Haas F, Vanderstraeten L, Becker D, Lang D, Vosolsobě S, Rombauts S, Wilhelmsson
P, Janitza P, Kern R, Heyl A, Rümpler F, Calderón Villalobos L, Clay J, Skokan
R, Toyoda A, Suzuki Y, Kagoshima H, Schijlen E, Tajeshwar N, Catarino B, Hetherington
A, Saltykova A, Bonnot C, Breuninger H, Symeonidi A, Radhakrishnan G, Van Nieuwerburgh
F, Deforce D, Chang C, Karol K, Hedrich R, Ulvskov P, Glöckner G, Delwiche C,
Petrášek J, Van De Peer Y, Friml J, Beilby M, Dolan L, Kohara Y, Sugano S, Fujiyama
A, Delaux PM, Quint M, Theissen G, Hagemann M, Harholt J, Dunand C, Zachgo S,
Langdale J, Maumus F, Van Der Straeten D, Gould SB, Rensing S. 2018. The Chara
genome: Secondary complexity and implications for plant terrestrialization. Cell.
174(2), 448–464.e24.'
mla: 'Nishiyama, Tomoaki, et al. “The Chara Genome: Secondary Complexity and Implications
for Plant Terrestrialization.” Cell, vol. 174, no. 2, Cell Press, 2018,
p. 448–464.e24, doi:10.1016/j.cell.2018.06.033.'
short: T. Nishiyama, H. Sakayama, J. De Vries, H. Buschmann, D. Saint Marcoux, K.
Ullrich, F. Haas, L. Vanderstraeten, D. Becker, D. Lang, S. Vosolsobě, S. Rombauts,
P. Wilhelmsson, P. Janitza, R. Kern, A. Heyl, F. Rümpler, L. Calderón Villalobos,
J. Clay, R. Skokan, A. Toyoda, Y. Suzuki, H. Kagoshima, E. Schijlen, N. Tajeshwar,
B. Catarino, A. Hetherington, A. Saltykova, C. Bonnot, H. Breuninger, A. Symeonidi,
G. Radhakrishnan, F. Van Nieuwerburgh, D. Deforce, C. Chang, K. Karol, R. Hedrich,
P. Ulvskov, G. Glöckner, C. Delwiche, J. Petrášek, Y. Van De Peer, J. Friml, M.
Beilby, L. Dolan, Y. Kohara, S. Sugano, A. Fujiyama, P.M. Delaux, M. Quint, G.
Theissen, M. Hagemann, J. Harholt, C. Dunand, S. Zachgo, J. Langdale, F. Maumus,
D. Van Der Straeten, S.B. Gould, S. Rensing, Cell 174 (2018) 448–464.e24.
date_created: 2018-12-11T11:44:53Z
date_published: 2018-07-12T00:00:00Z
date_updated: 2023-09-19T10:02:47Z
day: '12'
department:
- _id: JiFr
doi: 10.1016/j.cell.2018.06.033
ec_funded: 1
external_id:
isi:
- '000438482800019'
pmid:
- '30007417'
intvolume: ' 174'
isi: 1
issue: '2'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pubmed/30007417
month: '07'
oa: 1
oa_version: Published Version
page: 448 - 464.e24
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Cell
publication_status: published
publisher: Cell Press
publist_id: '7774'
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'The Chara genome: Secondary complexity and implications for plant terrestrialization'
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 174
year: '2018'
...
---
_id: '158'
abstract:
- lang: eng
text: 'The angiosperm seed is composed of three genetically distinct tissues: the
diploid embryo that originates from the fertilized egg cell, the triploid endosperm
that is produced from the fertilized central cell, and the maternal sporophytic
integuments that develop into the seed coat1. At the onset of embryo development
in Arabidopsis thaliana, the zygote divides asymmetrically, producing a small
apical embryonic cell and a larger basal cell that connects the embryo to the
maternal tissue2. The coordinated and synchronous development of the embryo and
the surrounding integuments, and the alignment of their growth axes, suggest communication
between maternal tissues and the embryo. In contrast to animals, however, where
a network of maternal factors that direct embryo patterning have been identified3,4,
only a few maternal mutations have been described to affect embryo development
in plants5–7. Early embryo patterning in Arabidopsis requires accumulation of
the phytohormone auxin in the apical cell by directed transport from the suspensor8–10.
However, the origin of this auxin has remained obscure. Here we investigate the
source of auxin for early embryogenesis and provide evidence that the mother plant
coordinates seed development by supplying auxin to the early embryo from the integuments
of the ovule. We show that auxin response increases in ovules after fertilization,
due to upregulated auxin biosynthesis in the integuments, and this maternally
produced auxin is required for correct embryo development.'
acknowledgement: This work was further supported by the Czech Science Foundation GACR
(GA13-40637S) to J.F.;
article_processing_charge: No
author:
- first_name: Hélène
full_name: Robert, Hélène
last_name: Robert
- first_name: Chulmin
full_name: Park, Chulmin
last_name: Park
- first_name: Carla
full_name: Gutièrrez, Carla
last_name: Gutièrrez
- first_name: Barbara
full_name: Wójcikowska, Barbara
last_name: Wójcikowska
- first_name: Aleš
full_name: Pěnčík, Aleš
last_name: Pěnčík
- first_name: Ondřej
full_name: Novák, Ondřej
last_name: Novák
- first_name: Junyi
full_name: Chen, Junyi
last_name: Chen
- first_name: Wim
full_name: Grunewald, Wim
last_name: Grunewald
- first_name: Thomas
full_name: Dresselhaus, Thomas
last_name: Dresselhaus
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Thomas
full_name: Laux, Thomas
last_name: Laux
citation:
ama: Robert H, Park C, Gutièrrez C, et al. Maternal auxin supply contributes to
early embryo patterning in Arabidopsis. Nature Plants. 2018;4(8):548-553.
doi:10.1038/s41477-018-0204-z
apa: Robert, H., Park, C., Gutièrrez, C., Wójcikowska, B., Pěnčík, A., Novák, O.,
… Laux, T. (2018). Maternal auxin supply contributes to early embryo patterning
in Arabidopsis. Nature Plants. Nature Publishing Group. https://doi.org/10.1038/s41477-018-0204-z
chicago: Robert, Hélène, Chulmin Park, Carla Gutièrrez, Barbara Wójcikowska, Aleš
Pěnčík, Ondřej Novák, Junyi Chen, et al. “Maternal Auxin Supply Contributes to
Early Embryo Patterning in Arabidopsis.” Nature Plants. Nature Publishing
Group, 2018. https://doi.org/10.1038/s41477-018-0204-z.
ieee: H. Robert et al., “Maternal auxin supply contributes to early embryo
patterning in Arabidopsis,” Nature Plants, vol. 4, no. 8. Nature Publishing
Group, pp. 548–553, 2018.
ista: Robert H, Park C, Gutièrrez C, Wójcikowska B, Pěnčík A, Novák O, Chen J, Grunewald
W, Dresselhaus T, Friml J, Laux T. 2018. Maternal auxin supply contributes to
early embryo patterning in Arabidopsis. Nature Plants. 4(8), 548–553.
mla: Robert, Hélène, et al. “Maternal Auxin Supply Contributes to Early Embryo Patterning
in Arabidopsis.” Nature Plants, vol. 4, no. 8, Nature Publishing Group,
2018, pp. 548–53, doi:10.1038/s41477-018-0204-z.
short: H. Robert, C. Park, C. Gutièrrez, B. Wójcikowska, A. Pěnčík, O. Novák, J.
Chen, W. Grunewald, T. Dresselhaus, J. Friml, T. Laux, Nature Plants 4 (2018)
548–553.
date_created: 2018-12-11T11:44:56Z
date_published: 2018-07-16T00:00:00Z
date_updated: 2025-05-07T11:12:31Z
day: '16'
department:
- _id: JiFr
doi: 10.1038/s41477-018-0204-z
ec_funded: 1
external_id:
isi:
- '000443861300011'
pmid:
- '30013211'
intvolume: ' 4'
isi: 1
issue: '8'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pubmed/30013211
month: '07'
oa: 1
oa_version: Submitted Version
page: 548 - 553
pmid: 1
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: Nature Plants
publication_status: published
publisher: Nature Publishing Group
publist_id: '7763'
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/plant-mothers-talk-to-their-embryos-via-the-hormone-auxin/
scopus_import: '1'
status: public
title: Maternal auxin supply contributes to early embryo patterning in Arabidopsis
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 4
year: '2018'
...
---
_id: '14'
abstract:
- lang: eng
text: The intercellular transport of auxin is driven by PIN-formed (PIN) auxin efflux
carriers. PINs are localized at the plasma membrane (PM) and on constitutively
recycling endomembrane vesicles. Therefore, PINs can mediate auxin transport either
by direct translocation across the PM or by pumping auxin into secretory vesicles
(SVs), leading to its secretory release upon fusion with the PM. Which of these
two mechanisms dominates is a matter of debate. Here, we addressed the issue with
a mathematical modeling approach. We demonstrate that the efficiency of secretory
transport depends on SV size, half-life of PINs on the PM, pH, exocytosis frequency
and PIN density. 3D structured illumination microscopy (SIM) was used to determine
PIN density on the PM. Combining this data with published values of the other
parameters, we show that the transport activity of PINs in SVs would have to be
at least 1000× greater than on the PM in order to produce a comparable macroscopic
auxin transport. If both transport mechanisms operated simultaneously and PINs
were equally active on SVs and PM, the contribution of secretion to the total
auxin flux would be negligible. In conclusion, while secretory vesicle-mediated
transport of auxin is an intriguing and theoretically possible model, it is unlikely
to be a major mechanism of auxin transport inplanta.
acknowledgement: 'European Research Council (ERC): 742985 to Jiri Friml; M.A. was
supported by the Austrian Science Fund (FWF) (M2379-B28); AJ was supported by the
Austria Science Fund (FWF): I03630 to Jiri Friml.'
article_processing_charge: No
article_type: original
author:
- first_name: Sander
full_name: Hille, Sander
last_name: Hille
- first_name: Maria
full_name: Akhmanova, Maria
id: 3425EC26-F248-11E8-B48F-1D18A9856A87
last_name: Akhmanova
orcid: 0000-0003-1522-3162
- first_name: Matous
full_name: Glanc, Matous
id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
last_name: Glanc
orcid: 0000-0003-0619-7783
- first_name: Alexander J
full_name: Johnson, Alexander J
id: 46A62C3A-F248-11E8-B48F-1D18A9856A87
last_name: Johnson
orcid: 0000-0002-2739-8843
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: 'Hille S, Akhmanova M, Glanc M, Johnson AJ, Friml J. Relative contribution
of PIN-containing secretory vesicles and plasma membrane PINs to the directed
auxin transport: Theoretical estimation. International Journal of Molecular
Sciences. 2018;19(11). doi:10.3390/ijms19113566'
apa: 'Hille, S., Akhmanova, M., Glanc, M., Johnson, A. J., & Friml, J. (2018).
Relative contribution of PIN-containing secretory vesicles and plasma membrane
PINs to the directed auxin transport: Theoretical estimation. International
Journal of Molecular Sciences. MDPI. https://doi.org/10.3390/ijms19113566'
chicago: 'Hille, Sander, Maria Akhmanova, Matous Glanc, Alexander J Johnson, and
Jiří Friml. “Relative Contribution of PIN-Containing Secretory Vesicles and Plasma
Membrane PINs to the Directed Auxin Transport: Theoretical Estimation.” International
Journal of Molecular Sciences. MDPI, 2018. https://doi.org/10.3390/ijms19113566.'
ieee: 'S. Hille, M. Akhmanova, M. Glanc, A. J. Johnson, and J. Friml, “Relative
contribution of PIN-containing secretory vesicles and plasma membrane PINs to
the directed auxin transport: Theoretical estimation,” International Journal
of Molecular Sciences, vol. 19, no. 11. MDPI, 2018.'
ista: 'Hille S, Akhmanova M, Glanc M, Johnson AJ, Friml J. 2018. Relative contribution
of PIN-containing secretory vesicles and plasma membrane PINs to the directed
auxin transport: Theoretical estimation. International Journal of Molecular Sciences.
19(11).'
mla: 'Hille, Sander, et al. “Relative Contribution of PIN-Containing Secretory Vesicles
and Plasma Membrane PINs to the Directed Auxin Transport: Theoretical Estimation.”
International Journal of Molecular Sciences, vol. 19, no. 11, MDPI, 2018,
doi:10.3390/ijms19113566.'
short: S. Hille, M. Akhmanova, M. Glanc, A.J. Johnson, J. Friml, International Journal
of Molecular Sciences 19 (2018).
date_created: 2018-12-11T11:44:09Z
date_published: 2018-11-12T00:00:00Z
date_updated: 2023-09-18T08:09:32Z
day: '12'
ddc:
- '580'
department:
- _id: DaSi
- _id: JiFr
doi: 10.3390/ijms19113566
ec_funded: 1
external_id:
isi:
- '000451528500282'
file:
- access_level: open_access
checksum: e4b59c2599b0ca26ebf5b8434bcde94a
content_type: application/pdf
creator: dernst
date_created: 2018-12-17T16:04:11Z
date_updated: 2020-07-14T12:44:50Z
file_id: '5719'
file_name: 2018_IJMS_Hille.pdf
file_size: 2200593
relation: main_file
file_date_updated: 2020-07-14T12:44:50Z
has_accepted_license: '1'
intvolume: ' 19'
isi: 1
issue: '11'
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
publication: International Journal of Molecular Sciences
publication_identifier:
eissn:
- 1422-0067
publication_status: published
publisher: MDPI
publist_id: '8042'
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Relative contribution of PIN-containing secretory vesicles and plasma membrane
PINs to the directed auxin transport: Theoretical estimation'
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 19
year: '2018'
...
---
_id: '913'
abstract:
- lang: eng
text: Coordinated cell polarization in developing tissues is a recurrent theme in
multicellular organisms. In plants, a directional distribution of the plant hormone
auxin is at the core of many developmental programs. A feedback regulation of
auxin on the polarized localization of PIN auxin transporters in individual cells
has been proposed as a self-organizing mechanism for coordinated tissue polarization,
but the molecular mechanisms linking auxin signalling to PIN-dependent auxin transport
remain unknown. We performed a microarray-based approach to find regulators of
the auxin-induced PIN relocation in the Arabidopsis thaliana root. We identified
a subset of a family of phosphatidylinositol transfer proteins (PITP), the PATELLINs
(PATL). Here, we show that PATLs are expressed in partially overlapping cells
types in different tissues going through mitosis or initiating differentiation
programs. PATLs are plasma membrane-associated proteins accumulated in Arabidopsis
embryos, primary roots, lateral root primordia, and developing stomata. Higher
order patl mutants display reduced PIN1 repolarization in response to auxin, shorter
root apical meristem, and drastic defects in embryo and seedling development.
This suggests PATLs redundantly play a crucial role in polarity and patterning
in Arabidopsis.
article_number: jcs.204198
article_processing_charge: No
author:
- first_name: Ricardo
full_name: Tejos, Ricardo
last_name: Tejos
- first_name: Cecilia
full_name: Rodríguez Furlán, Cecilia
last_name: Rodríguez Furlán
- first_name: Maciek
full_name: Adamowski, Maciek
id: 45F536D2-F248-11E8-B48F-1D18A9856A87
last_name: Adamowski
orcid: 0000-0001-6463-5257
- first_name: Michael
full_name: Sauer, Michael
last_name: Sauer
- first_name: Lorena
full_name: Norambuena, Lorena
last_name: Norambuena
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Tejos R, Rodríguez Furlán C, Adamowski M, Sauer M, Norambuena L, Friml J. PATELLINS
are regulators of auxin mediated PIN1 relocation and plant development in Arabidopsis
thaliana. Journal of Cell Science. 2018;131(2). doi:10.1242/jcs.204198
apa: Tejos, R., Rodríguez Furlán, C., Adamowski, M., Sauer, M., Norambuena, L.,
& Friml, J. (2018). PATELLINS are regulators of auxin mediated PIN1 relocation
and plant development in Arabidopsis thaliana. Journal of Cell Science.
Company of Biologists. https://doi.org/10.1242/jcs.204198
chicago: Tejos, Ricardo, Cecilia Rodríguez Furlán, Maciek Adamowski, Michael Sauer,
Lorena Norambuena, and Jiří Friml. “PATELLINS Are Regulators of Auxin Mediated
PIN1 Relocation and Plant Development in Arabidopsis Thaliana.” Journal of
Cell Science. Company of Biologists, 2018. https://doi.org/10.1242/jcs.204198.
ieee: R. Tejos, C. Rodríguez Furlán, M. Adamowski, M. Sauer, L. Norambuena, and
J. Friml, “PATELLINS are regulators of auxin mediated PIN1 relocation and plant
development in Arabidopsis thaliana,” Journal of Cell Science, vol. 131,
no. 2. Company of Biologists, 2018.
ista: Tejos R, Rodríguez Furlán C, Adamowski M, Sauer M, Norambuena L, Friml J.
2018. PATELLINS are regulators of auxin mediated PIN1 relocation and plant development
in Arabidopsis thaliana. Journal of Cell Science. 131(2), jcs. 204198.
mla: Tejos, Ricardo, et al. “PATELLINS Are Regulators of Auxin Mediated PIN1 Relocation
and Plant Development in Arabidopsis Thaliana.” Journal of Cell Science,
vol. 131, no. 2, jcs. 204198, Company of Biologists, 2018, doi:10.1242/jcs.204198.
short: R. Tejos, C. Rodríguez Furlán, M. Adamowski, M. Sauer, L. Norambuena, J.
Friml, Journal of Cell Science 131 (2018).
date_created: 2018-12-11T11:49:10Z
date_published: 2018-01-29T00:00:00Z
date_updated: 2025-05-07T11:12:29Z
day: '29'
ddc:
- '581'
department:
- _id: JiFr
doi: 10.1242/jcs.204198
ec_funded: 1
external_id:
isi:
- '000424842400019'
file:
- access_level: open_access
checksum: bf156c20a4f117b4b932370d54cbac8c
content_type: application/pdf
creator: dernst
date_created: 2019-04-12T08:46:32Z
date_updated: 2020-07-14T12:48:15Z
file_id: '6299'
file_name: 2017_adamowski_PATELLINS_are.pdf
file_size: 14925985
relation: main_file
file_date_updated: 2020-07-14T12:48:15Z
has_accepted_license: '1'
intvolume: ' 131'
isi: 1
issue: '2'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: Journal of Cell Science
publication_identifier:
issn:
- '00219533'
publication_status: published
publisher: Company of Biologists
publist_id: '6530'
pubrep_id: '988'
quality_controlled: '1'
scopus_import: '1'
status: public
title: PATELLINS are regulators of auxin mediated PIN1 relocation and plant development
in Arabidopsis thaliana
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 131
year: '2018'
...
---
_id: '36'
abstract:
- lang: eng
text: Wheat (Triticum ssp.) is one of the most important human food sources. However,
this crop is very sensitive to temperature changes. Specifically, processes during
wheat leaf, flower, and seed development and photosynthesis, which all contribute
to the yield of this crop, are affected by high temperature. While this has to
some extent been investigated on physiological, developmental, and molecular levels,
very little is known about early signalling events associated with an increase
in temperature. Phosphorylation-mediated signalling mechanisms, which are quick
and dynamic, are associated with plant growth and development, also under abiotic
stress conditions. Therefore, we probed the impact of a short-term and mild increase
in temperature on the wheat leaf and spikelet phosphoproteome. In total, 3822
(containing 5178 phosphosites) and 5581 phosphopeptides (containing 7023 phosphosites)
were identified in leaf and spikelet samples, respectively. Following statistical
analysis, the resulting data set provides the scientific community with a first
large-scale plant phosphoproteome under the control of higher ambient temperature.
This community resource on the high temperature-mediated wheat phosphoproteome
will be valuable for future studies. Our analyses also revealed a core set of
common proteins between leaf and spikelet, suggesting some level of conserved
regulatory mechanisms. Furthermore, we observed temperature-regulated interconversion
of phosphoforms, which probably impacts protein activity.
acknowledgement: TZ is supported by a grant from the Chinese Scholarship Council.
article_processing_charge: No
author:
- first_name: Lam
full_name: Vu, Lam
last_name: Vu
- first_name: Tingting
full_name: Zhu, Tingting
last_name: Zhu
- first_name: Inge
full_name: Verstraeten, Inge
id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
last_name: Verstraeten
orcid: 0000-0001-7241-2328
- first_name: Brigitte
full_name: Van De Cotte, Brigitte
last_name: Van De Cotte
- first_name: Kris
full_name: Gevaert, Kris
last_name: Gevaert
- first_name: Ive
full_name: De Smet, Ive
last_name: De Smet
citation:
ama: Vu L, Zhu T, Verstraeten I, Van De Cotte B, Gevaert K, De Smet I. Temperature-induced
changes in the wheat phosphoproteome reveal temperature-regulated interconversion
of phosphoforms. Journal of Experimental Botany. 2018;69(19):4609-4624.
doi:10.1093/jxb/ery204
apa: Vu, L., Zhu, T., Verstraeten, I., Van De Cotte, B., Gevaert, K., & De Smet,
I. (2018). Temperature-induced changes in the wheat phosphoproteome reveal temperature-regulated
interconversion of phosphoforms. Journal of Experimental Botany. Oxford
University Press. https://doi.org/10.1093/jxb/ery204
chicago: Vu, Lam, Tingting Zhu, Inge Verstraeten, Brigitte Van De Cotte, Kris Gevaert,
and Ive De Smet. “Temperature-Induced Changes in the Wheat Phosphoproteome Reveal
Temperature-Regulated Interconversion of Phosphoforms.” Journal of Experimental
Botany. Oxford University Press, 2018. https://doi.org/10.1093/jxb/ery204.
ieee: L. Vu, T. Zhu, I. Verstraeten, B. Van De Cotte, K. Gevaert, and I. De Smet,
“Temperature-induced changes in the wheat phosphoproteome reveal temperature-regulated
interconversion of phosphoforms,” Journal of Experimental Botany, vol.
69, no. 19. Oxford University Press, pp. 4609–4624, 2018.
ista: Vu L, Zhu T, Verstraeten I, Van De Cotte B, Gevaert K, De Smet I. 2018. Temperature-induced
changes in the wheat phosphoproteome reveal temperature-regulated interconversion
of phosphoforms. Journal of Experimental Botany. 69(19), 4609–4624.
mla: Vu, Lam, et al. “Temperature-Induced Changes in the Wheat Phosphoproteome Reveal
Temperature-Regulated Interconversion of Phosphoforms.” Journal of Experimental
Botany, vol. 69, no. 19, Oxford University Press, 2018, pp. 4609–24, doi:10.1093/jxb/ery204.
short: L. Vu, T. Zhu, I. Verstraeten, B. Van De Cotte, K. Gevaert, I. De Smet, Journal
of Experimental Botany 69 (2018) 4609–4624.
date_created: 2018-12-11T11:44:17Z
date_published: 2018-08-31T00:00:00Z
date_updated: 2023-09-19T10:00:46Z
day: '31'
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department:
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doi: 10.1093/jxb/ery204
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title: Temperature-induced changes in the wheat phosphoproteome reveal temperature-regulated
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