[{"publication_identifier":{"eisbn":["978-1-0716-1744-1"],"issn":["1064-3745"],"isbn":["978-1-0716-1743-4"],"eissn":["1940-6029"]},"language":[{"iso":"eng"}],"publication":"Plant Cell Division","day":"28","doi":"10.1007/978-1-0716-1744-1_6","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","alternative_title":["Methods in Molecular Biology"],"title":"Automated time-lapse imaging and manipulation of cell divisions in Arabidopsis roots by vertical-stage confocal microscopy","pmid":1,"acknowledged_ssus":[{"_id":"Bio"}],"acknowledgement":"We thank B. De Rybel for allowing M.G. to work on this manuscript during a postdoc in his laboratory, and EMBO for supporting M.G. with a Long-Term fellowship (ALTF 1005-2019) during this time. We acknowledge the service and support by the Bioimaging Facility at IST Austria, and finally, we thank A. Mally for proofreading and correcting the manuscript.","year":"2021","department":[{"_id":"JiFr"}],"article_processing_charge":"No","citation":{"chicago":"Hörmayer, Lukas, Jiří Friml, and Matous Glanc. “Automated Time-Lapse Imaging and Manipulation of Cell Divisions in Arabidopsis Roots by Vertical-Stage Confocal Microscopy.” In <i>Plant Cell Division</i>, 2382:105–14. MIMB. Humana Press, 2021. <a href=\"https://doi.org/10.1007/978-1-0716-1744-1_6\">https://doi.org/10.1007/978-1-0716-1744-1_6</a>.","apa":"Hörmayer, L., Friml, J., &#38; Glanc, M. (2021). Automated time-lapse imaging and manipulation of cell divisions in Arabidopsis roots by vertical-stage confocal microscopy. In <i>Plant Cell Division</i> (Vol. 2382, pp. 105–114). Humana Press. <a href=\"https://doi.org/10.1007/978-1-0716-1744-1_6\">https://doi.org/10.1007/978-1-0716-1744-1_6</a>","ieee":"L. Hörmayer, J. Friml, and M. Glanc, “Automated time-lapse imaging and manipulation of cell divisions in Arabidopsis roots by vertical-stage confocal microscopy,” in <i>Plant Cell Division</i>, vol. 2382, Humana Press, 2021, pp. 105–114.","ista":"Hörmayer L, Friml J, Glanc M. 2021.Automated time-lapse imaging and manipulation of cell divisions in Arabidopsis roots by vertical-stage confocal microscopy. In: Plant Cell Division. Methods in Molecular Biology, vol. 2382, 105–114.","ama":"Hörmayer L, Friml J, Glanc M. Automated time-lapse imaging and manipulation of cell divisions in Arabidopsis roots by vertical-stage confocal microscopy. In: <i>Plant Cell Division</i>. Vol 2382. MIMB. Humana Press; 2021:105-114. doi:<a href=\"https://doi.org/10.1007/978-1-0716-1744-1_6\">10.1007/978-1-0716-1744-1_6</a>","short":"L. Hörmayer, J. Friml, M. Glanc, in:, Plant Cell Division, Humana Press, 2021, pp. 105–114.","mla":"Hörmayer, Lukas, et al. “Automated Time-Lapse Imaging and Manipulation of Cell Divisions in Arabidopsis Roots by Vertical-Stage Confocal Microscopy.” <i>Plant Cell Division</i>, vol. 2382, Humana Press, 2021, pp. 105–14, doi:<a href=\"https://doi.org/10.1007/978-1-0716-1744-1_6\">10.1007/978-1-0716-1744-1_6</a>."},"publisher":"Humana Press","type":"book_chapter","series_title":"MIMB","date_updated":"2022-06-03T06:47:06Z","oa_version":"None","month":"10","status":"public","external_id":{"pmid":["34705235"]},"publication_status":"published","date_created":"2021-11-11T10:03:30Z","abstract":[{"text":"The analysis of dynamic cellular processes such as plant cytokinesis stands and falls with live-cell time-lapse confocal imaging. Conventional approaches to time-lapse imaging of cell division in Arabidopsis root tips are tedious and have low throughput. Here, we describe a protocol for long-term time-lapse simultaneous imaging of multiple root tips on a vertical-stage confocal microscope with automated root tracking. We also provide modifications of the basic protocol to implement this imaging method in the analysis of genetic, pharmacological or laser ablation wounding-mediated experimental manipulations. Our method dramatically improves the efficiency of cell division time-lapse imaging by increasing the throughput, while reducing the person-hour requirements of such experiments.","lang":"eng"}],"scopus_import":"1","date_published":"2021-10-28T00:00:00Z","intvolume":"      2382","author":[{"id":"2EEE7A2A-F248-11E8-B48F-1D18A9856A87","full_name":"Hörmayer, Lukas","first_name":"Lukas","last_name":"Hörmayer"},{"last_name":"Friml","full_name":"Friml, Jiří","first_name":"Jiří","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"id":"1AE1EA24-02D0-11E9-9BAA-DAF4881429F2","orcid":"0000-0003-0619-7783","first_name":"Matous","full_name":"Glanc, Matous","last_name":"Glanc"}],"volume":2382,"page":"105-114","quality_controlled":"1","_id":"10268"},{"_id":"10282","quality_controlled":"1","intvolume":"       233","date_published":"2021-11-05T00:00:00Z","volume":233,"page":"329-343","author":[{"last_name":"Kashkan","full_name":"Kashkan, Ivan","first_name":"Ivan"},{"id":"45A71A74-F248-11E8-B48F-1D18A9856A87","first_name":"Mónika","full_name":"Hrtyan, Mónika","last_name":"Hrtyan"},{"last_name":"Retzer","first_name":"Katarzyna","full_name":"Retzer, Katarzyna"},{"first_name":"Jana","full_name":"Humpolíčková, Jana","last_name":"Humpolíčková"},{"first_name":"Aswathy","full_name":"Jayasree, Aswathy","last_name":"Jayasree"},{"full_name":"Filepová, Roberta","first_name":"Roberta","last_name":"Filepová"},{"full_name":"Vondráková, Zuzana","first_name":"Zuzana","last_name":"Vondráková"},{"last_name":"Simon","full_name":"Simon, Sibu","first_name":"Sibu","orcid":"0000-0002-1998-6741","id":"4542EF9A-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Rombaut, Debbie","first_name":"Debbie","last_name":"Rombaut"},{"first_name":"Thomas B.","full_name":"Jacobs, Thomas B.","last_name":"Jacobs"},{"last_name":"Frilander","first_name":"Mikko J.","full_name":"Frilander, Mikko J."},{"last_name":"Hejátko","first_name":"Jan","full_name":"Hejátko, Jan"},{"first_name":"Jiří","orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml"},{"last_name":"Petrášek","first_name":"Jan","full_name":"Petrášek, Jan"},{"full_name":"Růžička, Kamil","first_name":"Kamil","last_name":"Růžička"}],"scopus_import":"1","abstract":[{"lang":"eng","text":"Advanced transcriptome sequencing has revealed that the majority of eukaryotic genes undergo alternative splicing (AS). Nonetheless, little effort has been dedicated to investigating the functional relevance of particular splicing events, even those in the key developmental and hormonal regulators. Combining approaches of genetics, biochemistry and advanced confocal microscopy, we describe the impact of alternative splicing on the PIN7 gene in the model plant Arabidopsis thaliana. PIN7 encodes a polarly localized transporter for the phytohormone auxin and produces two evolutionarily conserved transcripts, PIN7a and PIN7b. PIN7a and PIN7b, differing in a four amino acid stretch, exhibit almost identical expression patterns and subcellular localization. We reveal that they are closely associated and mutually influence each other's mobility within the plasma membrane. Phenotypic complementation tests indicate that the functional contribution of PIN7b per se is minor, but it markedly reduces the prominent PIN7a activity, which is required for correct seedling apical hook formation and auxin-mediated tropic responses. Our results establish alternative splicing of the PIN family as a conserved, functionally relevant mechanism, revealing an additional regulatory level of auxin-mediated plant development."}],"date_created":"2021-11-14T23:01:24Z","publication_status":"published","external_id":{"isi":["000714678100001"],"pmid":["34637542"]},"isi":1,"status":"public","month":"11","date_updated":"2023-08-14T11:46:43Z","oa_version":"Preprint","type":"journal_article","citation":{"short":"I. Kashkan, M. Hrtyan, K. Retzer, J. Humpolíčková, A. Jayasree, R. Filepová, Z. Vondráková, S. Simon, D. Rombaut, T.B. Jacobs, M.J. Frilander, J. Hejátko, J. Friml, J. Petrášek, K. Růžička, New Phytologist 233 (2021) 329–343.","mla":"Kashkan, Ivan, et al. “Mutually Opposing Activity of PIN7 Splicing Isoforms Is Required for Auxin-Mediated Tropic Responses in Arabidopsis Thaliana.” <i>New Phytologist</i>, vol. 233, Wiley, 2021, pp. 329–43, doi:<a href=\"https://doi.org/10.1111/nph.17792\">10.1111/nph.17792</a>.","ista":"Kashkan I, Hrtyan M, Retzer K, Humpolíčková J, Jayasree A, Filepová R, Vondráková Z, Simon S, Rombaut D, Jacobs TB, Frilander MJ, Hejátko J, Friml J, Petrášek J, Růžička K. 2021. Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana. New Phytologist. 233, 329–343.","ama":"Kashkan I, Hrtyan M, Retzer K, et al. Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana. <i>New Phytologist</i>. 2021;233:329-343. doi:<a href=\"https://doi.org/10.1111/nph.17792\">10.1111/nph.17792</a>","apa":"Kashkan, I., Hrtyan, M., Retzer, K., Humpolíčková, J., Jayasree, A., Filepová, R., … Růžička, K. (2021). Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana. <i>New Phytologist</i>. Wiley. <a href=\"https://doi.org/10.1111/nph.17792\">https://doi.org/10.1111/nph.17792</a>","ieee":"I. Kashkan <i>et al.</i>, “Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana,” <i>New Phytologist</i>, vol. 233. Wiley, pp. 329–343, 2021.","chicago":"Kashkan, Ivan, Mónika Hrtyan, Katarzyna Retzer, Jana Humpolíčková, Aswathy Jayasree, Roberta Filepová, Zuzana Vondráková, et al. “Mutually Opposing Activity of PIN7 Splicing Isoforms Is Required for Auxin-Mediated Tropic Responses in Arabidopsis Thaliana.” <i>New Phytologist</i>. Wiley, 2021. <a href=\"https://doi.org/10.1111/nph.17792\">https://doi.org/10.1111/nph.17792</a>."},"publisher":"Wiley","article_processing_charge":"No","year":"2021","department":[{"_id":"JiFr"}],"acknowledgement":"We thank Claus Schwechheimer for the pin34 and pin347 seeds, Yuliia Mironova for technical assistance, Ksenia Timofeyenko and Dmitry Konovalov for help with the evolutional analysis, Konstantin Kutashev and Siarhei Dabravolski for assistance with FRET-FLIM, Huibin Han for advice with hypocotyl imaging, Karel Müller for the initial qRT-PCR on the tobacco cell lines, Stano Pekár for suggestions regarding the statistical analysis of the morphodynamic measurements, and Jozef Mravec, Dolf Weijers and Lindy Abas for their comments on the manuscript. This work was supported by the Czech Science Foundation (projects 16-26428S and 19-23773S to IK, MH and KRůžička, 19-18917S to JHumpolíčková and 18-26981S to JF), and the Ministry of Education, Youth and Sports of the Czech Republic (MEYS, CZ.02.1.01/0.0/0.0/16_019/0000738) to KRůžička and JHejátko. The imaging facilities of the Institute of Experimental Botany and CEITEC are supported by MEYS (LM2018129 – Czech BioImaging and CZ.02.1.01/0.0/0.0/16_013/0001775). The authors declare no competing interests.","pmid":1,"main_file_link":[{"open_access":"1","url":"https://www.biorxiv.org/content/10.1101/2020.05.02.074070v2"}],"title":"Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","doi":"10.1111/nph.17792","publication":"New Phytologist","day":"05","article_type":"original","language":[{"iso":"eng"}],"publication_identifier":{"eissn":["1469-8137"],"issn":["0028-646X"]},"oa":1},{"external_id":{"isi":["000717408000002"],"pmid":["34764442"]},"date_created":"2021-11-21T23:01:30Z","publication_status":"published","abstract":[{"text":"Strigolactones (SLs) are carotenoid-derived plant hormones that control shoot branching and communications between host plants and symbiotic fungi or root parasitic plants. Extensive studies have identified the key components participating in SL biosynthesis and signalling, whereas the catabolism or deactivation of endogenous SLs in planta remains largely unknown. Here, we report that the Arabidopsis carboxylesterase 15 (AtCXE15) and its orthologues function as efficient hydrolases of SLs. We show that overexpression of AtCXE15 promotes shoot branching by dampening SL-inhibited axillary bud outgrowth. We further demonstrate that AtCXE15 could bind and efficiently hydrolyse SLs both in vitro and in planta. We also provide evidence that AtCXE15 is capable of catalysing hydrolysis of diverse SL analogues and that such CXE15-dependent catabolism of SLs is evolutionarily conserved in seed plants. These results disclose a catalytic mechanism underlying homoeostatic regulation of SLs in plants, which also provides a rational approach to spatial-temporally manipulate the endogenous SLs and thus architecture of crops and ornamental plants.","lang":"eng"}],"scopus_import":"1","volume":7,"author":[{"first_name":"Enjun","full_name":"Xu, Enjun","last_name":"Xu"},{"first_name":"Liang","full_name":"Chai, Liang","last_name":"Chai"},{"full_name":"Zhang, Shiqi","first_name":"Shiqi","last_name":"Zhang"},{"first_name":"Ruixue","full_name":"Yu, Ruixue","last_name":"Yu"},{"last_name":"Zhang","full_name":"Zhang, Xixi","first_name":"Xixi","id":"61A66458-47E9-11EA-85BA-8AEAAF14E49A","orcid":"0000-0001-7048-4627"},{"first_name":"Chongyi","full_name":"Xu, Chongyi","last_name":"Xu"},{"last_name":"Hu","full_name":"Hu, Yuxin","first_name":"Yuxin"}],"page":"1495–1504 ","date_published":"2021-11-11T00:00:00Z","intvolume":"         7","quality_controlled":"1","_id":"10326","article_processing_charge":"No","publisher":"Springer Nature","citation":{"apa":"Xu, E., Chai, L., Zhang, S., Yu, R., Zhang, X., Xu, C., &#38; Hu, Y. (2021). Catabolism of strigolactones by a carboxylesterase. <i>Nature Plants</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41477-021-01011-y\">https://doi.org/10.1038/s41477-021-01011-y</a>","ieee":"E. Xu <i>et al.</i>, “Catabolism of strigolactones by a carboxylesterase,” <i>Nature Plants</i>, vol. 7. Springer Nature, pp. 1495–1504, 2021.","chicago":"Xu, Enjun, Liang Chai, Shiqi Zhang, Ruixue Yu, Xixi Zhang, Chongyi Xu, and Yuxin Hu. “Catabolism of Strigolactones by a Carboxylesterase.” <i>Nature Plants</i>. Springer Nature, 2021. <a href=\"https://doi.org/10.1038/s41477-021-01011-y\">https://doi.org/10.1038/s41477-021-01011-y</a>.","mla":"Xu, Enjun, et al. “Catabolism of Strigolactones by a Carboxylesterase.” <i>Nature Plants</i>, vol. 7, Springer Nature, 2021, pp. 1495–1504, doi:<a href=\"https://doi.org/10.1038/s41477-021-01011-y\">10.1038/s41477-021-01011-y</a>.","short":"E. Xu, L. Chai, S. Zhang, R. Yu, X. Zhang, C. Xu, Y. Hu, Nature Plants 7 (2021) 1495–1504.","ama":"Xu E, Chai L, Zhang S, et al. Catabolism of strigolactones by a carboxylesterase. <i>Nature Plants</i>. 2021;7:1495–1504. doi:<a href=\"https://doi.org/10.1038/s41477-021-01011-y\">10.1038/s41477-021-01011-y</a>","ista":"Xu E, Chai L, Zhang S, Yu R, Zhang X, Xu C, Hu Y. 2021. Catabolism of strigolactones by a carboxylesterase. Nature Plants. 7, 1495–1504."},"type":"journal_article","oa_version":"None","date_updated":"2023-08-14T11:54:02Z","month":"11","status":"public","isi":1,"title":"Catabolism of strigolactones by a carboxylesterase","pmid":1,"acknowledgement":"We thank J. Li (Institute of Genetics and Developmental Biology, China) for providing the at14-1, atmax2-1, atmax3-9, atmax4-1, atmax1-1, kai2-2 (Col-0 background) mutants and B. Xu for providing the complementary DNA of P. patens. We are grateful to L. Wang for assistance with MST, B. Han for assistance with UPLC–MS, J. Li for assistance with confocal microscopy and B. Mikael and J. Zhang for their comments on the manuscript. This work was supported by grants from Strategic Priority Research Program of Chinese Academy of Sciences (Y.H., XDB27030102) and the National Natural Science Foundation of China (E.X., 31700253; Y.H., 31830055).","department":[{"_id":"JiFr"}],"year":"2021","publication_identifier":{"eissn":["2055-0278"]},"language":[{"iso":"eng"}],"article_type":"original","day":"11","publication":"Nature Plants","doi":"10.1038/s41477-021-01011-y","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8"},{"oa":1,"publication_identifier":{"issn":["1040-4651"],"eissn":["1532-298x"]},"language":[{"iso":"eng"}],"ec_funded":1,"article_type":"original","day":"01","publication":"Plant Cell","doi":"10.1093/plcell/koab122","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","title":"Synaptotagmins at the endoplasmic reticulum-plasma membrane contact sites maintain diacylglycerol homeostasis during abiotic stress","pmid":1,"acknowledgement":"We would also like to thank Lothar Willmitzer for the lipidomic analysis at the Max Planck Institute of Molecular Plant Physiology (Potsdam, Germany). We thank Manuela Vega from SCI for her technical assistance in image analysis. We thank John R. Pearson and the Bionand Nanoimaging Unit, F. David Navas Fernández and the SCAI Imaging Facility and The Plant Cell Biology facility at the Shanghai Center for Plant Stress Biology for assistance with confocal microscopy. The FaFAH1 clone was a gift from Iraida Amaya Saavedra (IFAPA-Centro de Churriana, Málaga, Spain). The AHA3 antibody against the H+-ATPase was a gift from Ramón Serrano Salom (Instituto de Biología Molecular y Celular de Plantas, Valencia, Spain). The MAP-mTU2-SAC1 construct was provided by Yvon Jaillais (Laboratoire Reproduction et Développement des Plantes, Univ Lyon, France). The pGWB5 from the pGWB vector series, was provided by Tsuyoshi Nakagawa (Department of Molecular and Functional Genomics, Shimane University). We thank Plan Propio from the University of Málaga for financial support.\r\nFunding","project":[{"call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","_id":"261099A6-B435-11E9-9278-68D0E5697425","grant_number":"742985"}],"department":[{"_id":"JiFr"}],"year":"2021","article_processing_charge":"No","has_accepted_license":"1","publisher":"American Society of Plant Biologists","citation":{"ista":"Ruiz-Lopez N, Pérez-Sancho J, Esteban Del Valle A, Haslam R, Vanneste S, Catalá R, Perea-Resa C, Van Damme D, García-Hernández S, Albert A, Vallarino J, Lin J, Friml J, Macho A, Salinas J, Rosado A, Napier J, Amorim-Silva V, Botella M. 2021. Synaptotagmins at the endoplasmic reticulum-plasma membrane contact sites maintain diacylglycerol homeostasis during abiotic stress. Plant Cell. 33(7), 2431–2453.","ama":"Ruiz-Lopez N, Pérez-Sancho J, Esteban Del Valle A, et al. Synaptotagmins at the endoplasmic reticulum-plasma membrane contact sites maintain diacylglycerol homeostasis during abiotic stress. <i>Plant Cell</i>. 2021;33(7):2431-2453. doi:<a href=\"https://doi.org/10.1093/plcell/koab122\">10.1093/plcell/koab122</a>","short":"N. Ruiz-Lopez, J. Pérez-Sancho, A. Esteban Del Valle, R. Haslam, S. Vanneste, R. Catalá, C. Perea-Resa, D. Van Damme, S. García-Hernández, A. Albert, J. Vallarino, J. Lin, J. Friml, A. Macho, J. Salinas, A. Rosado, J. Napier, V. Amorim-Silva, M. Botella, Plant Cell 33 (2021) 2431–2453.","mla":"Ruiz-Lopez, N., et al. “Synaptotagmins at the Endoplasmic Reticulum-Plasma Membrane Contact Sites Maintain Diacylglycerol Homeostasis during Abiotic Stress.” <i>Plant Cell</i>, vol. 33, no. 7, American Society of Plant Biologists, 2021, pp. 2431–53, doi:<a href=\"https://doi.org/10.1093/plcell/koab122\">10.1093/plcell/koab122</a>.","chicago":"Ruiz-Lopez, N, J Pérez-Sancho, A Esteban Del Valle, RP Haslam, S Vanneste, R Catalá, C Perea-Resa, et al. “Synaptotagmins at the Endoplasmic Reticulum-Plasma Membrane Contact Sites Maintain Diacylglycerol Homeostasis during Abiotic Stress.” <i>Plant Cell</i>. American Society of Plant Biologists, 2021. <a href=\"https://doi.org/10.1093/plcell/koab122\">https://doi.org/10.1093/plcell/koab122</a>.","ieee":"N. Ruiz-Lopez <i>et al.</i>, “Synaptotagmins at the endoplasmic reticulum-plasma membrane contact sites maintain diacylglycerol homeostasis during abiotic stress,” <i>Plant Cell</i>, vol. 33, no. 7. American Society of Plant Biologists, pp. 2431–2453, 2021.","apa":"Ruiz-Lopez, N., Pérez-Sancho, J., Esteban Del Valle, A., Haslam, R., Vanneste, S., Catalá, R., … Botella, M. (2021). Synaptotagmins at the endoplasmic reticulum-plasma membrane contact sites maintain diacylglycerol homeostasis during abiotic stress. <i>Plant Cell</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1093/plcell/koab122\">https://doi.org/10.1093/plcell/koab122</a>"},"type":"journal_article","file":[{"file_name":"2021_PlantCell_RuizLopez.pdf","file_size":2952028,"success":1,"content_type":"application/pdf","date_updated":"2021-10-14T13:36:38Z","relation":"main_file","file_id":"10141","checksum":"22d596678d00310d793611864a6d0fcd","creator":"cchlebak","date_created":"2021-10-14T13:36:38Z","access_level":"open_access"}],"tmp":{"image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"oa_version":"Published Version","date_updated":"2023-08-08T13:54:32Z","status":"public","month":"07","ddc":["580"],"isi":1,"date_created":"2021-06-02T13:13:58Z","publication_status":"published","external_id":{"pmid":["33944955"],"isi":["000703938100026"]},"abstract":[{"lang":"eng","text":"Endoplasmic reticulum–plasma membrane contact sites (ER–PM CS) play fundamental roles in all eukaryotic cells. Arabidopsis thaliana mutants lacking the ER–PM protein tether synaptotagmin1 (SYT1) exhibit decreased PM integrity under multiple abiotic stresses, such as freezing, high salt, osmotic stress, and mechanical damage. Here, we show that, together with SYT1, the stress-induced SYT3 is an ER–PM tether that also functions in maintaining PM integrity. The ER–PM CS localization of SYT1 and SYT3 is dependent on PM phosphatidylinositol-4-phosphate and is regulated by abiotic stress. Lipidomic analysis revealed that cold stress increased the accumulation of diacylglycerol at the PM in a syt1/3 double mutant relative to wild-type while the levels of most glycerolipid species remain unchanged. In addition, the SYT1-green fluorescent protein fusion preferentially binds diacylglycerol in vivo with little affinity for polar glycerolipids. Our work uncovers a SYT-dependent mechanism of stress adaptation counteracting the detrimental accumulation of diacylglycerol at the PM produced during episodes of abiotic stress."}],"file_date_updated":"2021-10-14T13:36:38Z","scopus_import":"1","volume":33,"author":[{"last_name":"Ruiz-Lopez","first_name":"N","full_name":"Ruiz-Lopez, N"},{"full_name":"Pérez-Sancho, J","first_name":"J","last_name":"Pérez-Sancho"},{"last_name":"Esteban Del Valle","first_name":"A","full_name":"Esteban Del Valle, A"},{"last_name":"Haslam","full_name":"Haslam, RP","first_name":"RP"},{"last_name":"Vanneste","full_name":"Vanneste, S","first_name":"S"},{"full_name":"Catalá, R","first_name":"R","last_name":"Catalá"},{"full_name":"Perea-Resa, C","first_name":"C","last_name":"Perea-Resa"},{"full_name":"Van Damme, D","first_name":"D","last_name":"Van Damme"},{"first_name":"S","full_name":"García-Hernández, S","last_name":"García-Hernández"},{"last_name":"Albert","full_name":"Albert, A","first_name":"A"},{"last_name":"Vallarino","full_name":"Vallarino, J","first_name":"J"},{"first_name":"J","full_name":"Lin, J","last_name":"Lin"},{"orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml"},{"last_name":"Macho","full_name":"Macho, AP","first_name":"AP"},{"last_name":"Salinas","first_name":"J","full_name":"Salinas, J"},{"last_name":"Rosado","full_name":"Rosado, A","first_name":"A"},{"last_name":"Napier","full_name":"Napier, JA","first_name":"JA"},{"last_name":"Amorim-Silva","first_name":"V","full_name":"Amorim-Silva, V"},{"first_name":"MA","full_name":"Botella, MA","last_name":"Botella"}],"page":"2431-2453","intvolume":"        33","date_published":"2021-07-01T00:00:00Z","issue":"7","quality_controlled":"1","_id":"9443"},{"quality_controlled":"1","issue":"2","_id":"9656","intvolume":"       232","date_published":"2021-10-01T00:00:00Z","page":"510-522","author":[{"first_name":"Huibin","full_name":"Han, Huibin","id":"31435098-F248-11E8-B48F-1D18A9856A87","last_name":"Han"},{"orcid":"0000-0001-6463-5257","full_name":"Adamowski, Maciek","id":"45F536D2-F248-11E8-B48F-1D18A9856A87","first_name":"Maciek","last_name":"Adamowski"},{"id":"44B04502-A9ED-11E9-B6FC-583AE6697425","first_name":"Linlin","full_name":"Qi, Linlin","orcid":"0000-0001-5187-8401","last_name":"Qi"},{"first_name":"SS","full_name":"Alotaibi, SS","last_name":"Alotaibi"},{"last_name":"Friml","full_name":"Friml, Jiří","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596"}],"volume":232,"scopus_import":"1","publication_status":"published","external_id":{"pmid":["34254313"],"isi":["000680587100001"]},"date_created":"2021-07-14T15:29:14Z","file_date_updated":"2021-10-07T13:42:47Z","abstract":[{"text":"Tropisms, growth responses to environmental stimuli such as light or gravity, are spectacular examples of adaptive plant development. The plant hormone auxin serves as a major coordinative signal. The PIN auxin exporters, through their dynamic polar subcellular localizations, redirect auxin fluxes in response to environmental stimuli and the resulting auxin gradients across organs underly differential cell elongation and bending. In this review, we discuss recent advances concerning regulations of PIN polarity during tropisms, focusing on PIN phosphorylation and trafficking. We also cover how environmental cues regulate PIN actions during tropisms, and a crucial role of auxin feedback on PIN polarity during bending termination. Finally, the interactions between different tropisms are reviewed to understand plant adaptive growth in the natural environment.","lang":"eng"}],"date_updated":"2023-08-10T14:02:41Z","oa_version":"Published Version","ddc":["580"],"isi":1,"month":"10","status":"public","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"file":[{"content_type":"application/pdf","date_updated":"2021-10-07T13:42:47Z","file_size":1939800,"success":1,"file_name":"2021_NewPhytologist_Han.pdf","relation":"main_file","file_id":"10105","access_level":"open_access","date_created":"2021-10-07T13:42:47Z","checksum":"6422a6eb329b52d96279daaee0fcf189","creator":"kschuh"}],"type":"journal_article","article_processing_charge":"Yes (via OA deal)","citation":{"chicago":"Han, Huibin, Maciek Adamowski, Linlin Qi, SS Alotaibi, and Jiří Friml. “PIN-Mediated Polar Auxin Transport Regulations in Plant Tropic Responses.” <i>New Phytologist</i>. Wiley, 2021. <a href=\"https://doi.org/10.1111/nph.17617\">https://doi.org/10.1111/nph.17617</a>.","apa":"Han, H., Adamowski, M., Qi, L., Alotaibi, S., &#38; Friml, J. (2021). PIN-mediated polar auxin transport regulations in plant tropic responses. <i>New Phytologist</i>. Wiley. <a href=\"https://doi.org/10.1111/nph.17617\">https://doi.org/10.1111/nph.17617</a>","ieee":"H. Han, M. Adamowski, L. Qi, S. Alotaibi, and J. Friml, “PIN-mediated polar auxin transport regulations in plant tropic responses,” <i>New Phytologist</i>, vol. 232, no. 2. Wiley, pp. 510–522, 2021.","ama":"Han H, Adamowski M, Qi L, Alotaibi S, Friml J. PIN-mediated polar auxin transport regulations in plant tropic responses. <i>New Phytologist</i>. 2021;232(2):510-522. doi:<a href=\"https://doi.org/10.1111/nph.17617\">10.1111/nph.17617</a>","ista":"Han H, Adamowski M, Qi L, Alotaibi S, Friml J. 2021. PIN-mediated polar auxin transport regulations in plant tropic responses. New Phytologist. 232(2), 510–522.","short":"H. Han, M. Adamowski, L. Qi, S. Alotaibi, J. Friml, New Phytologist 232 (2021) 510–522.","mla":"Han, Huibin, et al. “PIN-Mediated Polar Auxin Transport Regulations in Plant Tropic Responses.” <i>New Phytologist</i>, vol. 232, no. 2, Wiley, 2021, pp. 510–22, doi:<a href=\"https://doi.org/10.1111/nph.17617\">10.1111/nph.17617</a>."},"has_accepted_license":"1","publisher":"Wiley","project":[{"call_identifier":"H2020","_id":"261099A6-B435-11E9-9278-68D0E5697425","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","grant_number":"742985"},{"call_identifier":"FWF","name":"Molecular mechanisms of endocytic cargo recognition in plants","_id":"26538374-B435-11E9-9278-68D0E5697425","grant_number":"I03630"}],"acknowledgement":"We are grateful to Lukas Fiedler, Alexandra Mally (IST Austria) and Dr. Bartel Vanholme (VIB, Ghent) for their critical comments on the manuscript. We apologize to those researchers whose great work was not cited. This work is supported by the European Research Council under the European Union’s Horizon 2020 research and innovation Programme (ERC grant agreement number 742985), and the Austrian Science Fund (FWF, grant number I 3630-B25) to JF. HH is supported by the China Scholarship Council (CSC scholarship, 201506870018) and a starting grant from Jiangxi Agriculture University (9232308314).","year":"2021","department":[{"_id":"JiFr"}],"pmid":1,"title":"PIN-mediated polar auxin transport regulations in plant tropic responses","doi":"10.1111/nph.17617","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","publication":"New Phytologist","day":"01","ec_funded":1,"article_type":"original","oa":1,"publication_identifier":{"issn":["0028-646x"],"eissn":["1469-8137"]},"language":[{"iso":"eng"}]},{"day":"07","publication":"Plant Cell","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","doi":"10.1093/plcell/koab183","publication_identifier":{"issn":["1040-4651"],"eissn":["1532-298x"]},"language":[{"iso":"eng"}],"oa":1,"article_type":"original","pmid":1,"department":[{"_id":"JiFr"}],"year":"2021","title":"GmPIN-dependent polar auxin transport is involved in soybean nodule development","file":[{"checksum":"6715712ec306c321f0204c817b7f8ae7","creator":"cziletti","date_created":"2021-07-19T12:13:34Z","access_level":"open_access","file_id":"9691","relation":"main_file","file_name":"2021_PlantCell_Gao.pdf","file_size":10566921,"success":1,"date_updated":"2021-07-19T12:13:34Z","content_type":"application/pdf"}],"tmp":{"image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"status":"public","month":"07","ddc":["580"],"isi":1,"oa_version":"Published Version","date_updated":"2023-08-10T14:01:41Z","has_accepted_license":"1","publisher":"American Society of Plant Biologists","citation":{"chicago":"Gao, Z, Z Chen, Y Cui, M Ke, H Xu, Q Xu, J Chen, et al. “GmPIN-Dependent Polar Auxin Transport Is Involved in Soybean Nodule Development.” <i>Plant Cell</i>. American Society of Plant Biologists, 2021. <a href=\"https://doi.org/10.1093/plcell/koab183\">https://doi.org/10.1093/plcell/koab183</a>.","apa":"Gao, Z., Chen, Z., Cui, Y., Ke, M., Xu, H., Xu, Q., … Chen, X. (2021). GmPIN-dependent polar auxin transport is involved in soybean nodule development. <i>Plant Cell</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1093/plcell/koab183\">https://doi.org/10.1093/plcell/koab183</a>","ieee":"Z. Gao <i>et al.</i>, “GmPIN-dependent polar auxin transport is involved in soybean nodule development,” <i>Plant Cell</i>, vol. 33, no. 9. American Society of Plant Biologists, pp. 2981–3003, 2021.","ama":"Gao Z, Chen Z, Cui Y, et al. GmPIN-dependent polar auxin transport is involved in soybean nodule development. <i>Plant Cell</i>. 2021;33(9):2981–3003. doi:<a href=\"https://doi.org/10.1093/plcell/koab183\">10.1093/plcell/koab183</a>","ista":"Gao Z, Chen Z, Cui Y, Ke M, Xu H, Xu Q, Chen J, Li Y, Huang L, Zhao H, Huang D, Mai S, Xu T, Liu X, Li S, Guan Y, Yang W, Friml J, Petrášek J, Zhang J, Chen X. 2021. GmPIN-dependent polar auxin transport is involved in soybean nodule development. Plant Cell. 33(9), 2981–3003.","short":"Z. Gao, Z. Chen, Y. Cui, M. Ke, H. Xu, Q. Xu, J. Chen, Y. Li, L. Huang, H. Zhao, D. Huang, S. Mai, T. Xu, X. Liu, S. Li, Y. Guan, W. Yang, J. Friml, J. Petrášek, J. Zhang, X. Chen, Plant Cell 33 (2021) 2981–3003.","mla":"Gao, Z., et al. “GmPIN-Dependent Polar Auxin Transport Is Involved in Soybean Nodule Development.” <i>Plant Cell</i>, vol. 33, no. 9, American Society of Plant Biologists, 2021, pp. 2981–3003, doi:<a href=\"https://doi.org/10.1093/plcell/koab183\">10.1093/plcell/koab183</a>."},"article_processing_charge":"No","type":"journal_article","volume":33,"page":"2981–3003","author":[{"first_name":"Z","full_name":"Gao, Z","last_name":"Gao"},{"full_name":"Chen, Z","first_name":"Z","last_name":"Chen"},{"last_name":"Cui","first_name":"Y","full_name":"Cui, Y"},{"first_name":"M","full_name":"Ke, M","last_name":"Ke"},{"last_name":"Xu","full_name":"Xu, H","first_name":"H"},{"last_name":"Xu","full_name":"Xu, Q","first_name":"Q"},{"last_name":"Chen","first_name":"J","full_name":"Chen, J"},{"last_name":"Li","first_name":"Y","full_name":"Li, Y"},{"last_name":"Huang","first_name":"L","full_name":"Huang, L"},{"last_name":"Zhao","full_name":"Zhao, H","first_name":"H"},{"full_name":"Huang, D","first_name":"D","last_name":"Huang"},{"last_name":"Mai","full_name":"Mai, S","first_name":"S"},{"full_name":"Xu, T","first_name":"T","last_name":"Xu"},{"last_name":"Liu","first_name":"X","full_name":"Liu, X"},{"last_name":"Li","first_name":"S","full_name":"Li, S"},{"first_name":"Y","full_name":"Guan, Y","last_name":"Guan"},{"last_name":"Yang","first_name":"W","full_name":"Yang, W"},{"last_name":"Friml","full_name":"Friml, Jiří","first_name":"Jiří","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"first_name":"J","full_name":"Petrášek, J","last_name":"Petrášek"},{"last_name":"Zhang","first_name":"J","full_name":"Zhang, J"},{"first_name":"X","full_name":"Chen, X","last_name":"Chen"}],"date_published":"2021-07-07T00:00:00Z","intvolume":"        33","_id":"9657","quality_controlled":"1","issue":"9","abstract":[{"text":"To overcome nitrogen deficiency, legume roots establish symbiotic interactions with nitrogen-fixing rhizobia that is fostered in specialized organs (nodules). Similar to other organs, nodule formation is determined by a local maximum of the phytohormone auxin at the primordium site. However, how auxin regulates nodule development remains poorly understood. Here, we found that in soybean, (Glycine max), dynamic auxin transport driven by PIN-FORMED (PIN) transporter GmPIN1 is involved in nodule primordium formation. GmPIN1 was specifically expressed in nodule primordium cells and GmPIN1 was polarly localized in these cells. Two nodulation regulators, (iso)flavonoids trigger expanded distribution of GmPIN1b to root cortical cells, and cytokinin rearranges GmPIN1b polarity. Gmpin1abc triple mutants generated with CRISPR-Cas9 showed impaired establishment of auxin maxima in nodule meristems and aberrant divisions in the nodule primordium cells. Moreover, overexpression of GmPIN1 suppressed nodule primordium initiation. GmPIN9d, an ortholog of Arabidopsis thaliana PIN2, acts together with GmPIN1 later in nodule development to acropetally transport auxin in vascular bundles, fine-tuning the auxin supply for nodule enlargement. Our findings reveal how PIN-dependent auxin transport modulates different aspects of soybean nodule development and suggest that establishment of auxin gradient is a prerequisite for the proper interaction between legumes and rhizobia.","lang":"eng"}],"file_date_updated":"2021-07-19T12:13:34Z","publication_status":"published","date_created":"2021-07-14T15:32:43Z","external_id":{"pmid":["34240197"],"isi":["000702165300012"]}},{"department":[{"_id":"JiFr"},{"_id":"MaLo"},{"_id":"EvBe"},{"_id":"EM-Fac"},{"_id":"NanoFab"}],"year":"2021","acknowledgement":"We gratefully thank Julie Neveu and Dr. Amanda Barranco of the Grégory Vert laboratory for help preparing plants in France, Dr. Zuzana Gelova for help and advice with protoplast generation, Dr. Stéphane Vassilopoulos and Dr. Florian Schur for advice regarding EM tomography, Alejandro Marquiegui Alvaro for help with material generation, and Dr. Lukasz Kowalski for generously gifting us the mWasabi protein. This research was supported by the Scientific Service Units of Institute of Science and Technology Austria (IST Austria) through resources provided by the Electron Microscopy Facility, Lab Support Facility (particularly Dorota Jaworska), and the Bioimaging Facility. We acknowledge the Advanced Microscopy Facility of the Vienna BioCenter Core Facilities for use of the 3D SIM. For the mass spectrometry analysis of proteins, we acknowledge the University of Natural Resources and Life Sciences (BOKU) Core Facility Mass Spectrometry. This work was supported by the following funds: A.J. is supported by funding from the Austrian Science Fund I3630B25 to J.F. P.M. and E.B. are supported by Agence Nationale de la Recherche ANR-11-EQPX-0029 Morphoscope2 and ANR-10-INBS-04 France BioImaging. S.Y.B. is supported by the NSF No. 1121998 and 1614915. J.W. and D.V.D. are supported by the European Research Council Grant 682436 (to D.V.D.), a China Scholarship Council Grant 201508440249 (to J.W.), and by a Ghent University Special Research Co-funding Grant ST01511051 (to J.W.).","project":[{"_id":"26538374-B435-11E9-9278-68D0E5697425","name":"Molecular mechanisms of endocytic cargo recognition in plants","grant_number":"I03630","call_identifier":"FWF"}],"pmid":1,"acknowledged_ssus":[{"_id":"EM-Fac"},{"_id":"LifeSc"},{"_id":"Bio"}],"title":"The TPLATE complex mediates membrane bending during plant clathrin-mediated endocytosis","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","doi":"10.1073/pnas.2113046118","day":"14","publication":"Proceedings of the National Academy of Sciences","article_type":"original","article_number":"e2113046118","publication_identifier":{"eissn":["1091-6490"]},"language":[{"iso":"eng"}],"oa":1,"_id":"9887","issue":"51","quality_controlled":"1","volume":118,"author":[{"orcid":"0000-0002-2739-8843","id":"46A62C3A-F248-11E8-B48F-1D18A9856A87","first_name":"Alexander J","full_name":"Johnson, Alexander J","last_name":"Johnson"},{"full_name":"Dahhan, Dana A","first_name":"Dana A","last_name":"Dahhan"},{"last_name":"Gnyliukh","orcid":"0000-0002-2198-0509","full_name":"Gnyliukh, Nataliia","first_name":"Nataliia","id":"390C1120-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Kaufmann","orcid":"0000-0001-9735-5315","first_name":"Walter","full_name":"Kaufmann, Walter","id":"3F99E422-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Zheden","first_name":"Vanessa","full_name":"Zheden, Vanessa","id":"39C5A68A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-9438-4783"},{"last_name":"Costanzo","first_name":"Tommaso","orcid":"0000-0001-9732-3815","id":"D93824F4-D9BA-11E9-BB12-F207E6697425","full_name":"Costanzo, Tommaso"},{"first_name":"Pierre","full_name":"Mahou, Pierre","last_name":"Mahou"},{"id":"45A71A74-F248-11E8-B48F-1D18A9856A87","full_name":"Hrtyan, Mónika","first_name":"Mónika","last_name":"Hrtyan"},{"last_name":"Wang","full_name":"Wang, Jie","first_name":"Jie"},{"id":"2A67C376-F248-11E8-B48F-1D18A9856A87","first_name":"Juan L","full_name":"Aguilera Servin, Juan L","orcid":"0000-0002-2862-8372","last_name":"Aguilera Servin"},{"full_name":"van Damme, Daniël","first_name":"Daniël","last_name":"van Damme"},{"last_name":"Beaurepaire","full_name":"Beaurepaire, Emmanuel","first_name":"Emmanuel"},{"first_name":"Martin","full_name":"Loose, Martin","orcid":"0000-0001-7309-9724","id":"462D4284-F248-11E8-B48F-1D18A9856A87","last_name":"Loose"},{"full_name":"Bednarek, Sebastian Y","first_name":"Sebastian Y","last_name":"Bednarek"},{"last_name":"Friml","orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"intvolume":"       118","date_published":"2021-12-14T00:00:00Z","abstract":[{"lang":"eng","text":"Clathrin-mediated endocytosis is the major route of entry of cargos into cells and thus underpins many physiological processes. During endocytosis, an area of flat membrane is remodeled by proteins to create a spherical vesicle against intracellular forces. The protein machinery which mediates this membrane bending in plants is unknown. However, it is known that plant endocytosis is actin independent, thus indicating that plants utilize a unique mechanism to mediate membrane bending against high-turgor pressure compared to other model systems. Here, we investigate the TPLATE complex, a plant-specific endocytosis protein complex. It has been thought to function as a classical adaptor functioning underneath the clathrin coat. However, by using biochemical and advanced live microscopy approaches, we found that TPLATE is peripherally associated with clathrin-coated vesicles and localizes at the rim of endocytosis events. As this localization is more fitting to the protein machinery involved in membrane bending during endocytosis, we examined cells in which the TPLATE complex was disrupted and found that the clathrin structures present as flat patches. This suggests a requirement of the TPLATE complex for membrane bending during plant clathrin–mediated endocytosis. Next, we used in vitro biophysical assays to confirm that the TPLATE complex possesses protein domains with intrinsic membrane remodeling activity. These results redefine the role of the TPLATE complex and implicate it as a key component of the evolutionarily distinct plant endocytosis mechanism, which mediates endocytic membrane bending against the high-turgor pressure in plant cells."}],"file_date_updated":"2021-12-15T08:59:40Z","date_created":"2021-08-11T14:11:43Z","publication_status":"published","external_id":{"pmid":["34907016"],"isi":["000736417600043"]},"month":"12","status":"public","isi":1,"ddc":["580"],"oa_version":"Published Version","date_updated":"2024-02-19T11:06:09Z","file":[{"file_id":"10546","access_level":"open_access","date_created":"2021-12-15T08:59:40Z","checksum":"8d01e72e22c4fb1584e72d8601947069","creator":"cchlebak","date_updated":"2021-12-15T08:59:40Z","content_type":"application/pdf","file_size":2757340,"success":1,"file_name":"2021_PNAS_Johnson.pdf","relation":"main_file"}],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"related_material":{"link":[{"relation":"earlier_version","url":"https://doi.org/10.1101/2021.04.26.441441"}],"record":[{"status":"public","relation":"dissertation_contains","id":"14510"},{"status":"public","id":"14988","relation":"research_data"}]},"type":"journal_article","publisher":"National Academy of Sciences","has_accepted_license":"1","citation":{"mla":"Johnson, Alexander J., et al. “The TPLATE Complex Mediates Membrane Bending during Plant Clathrin-Mediated Endocytosis.” <i>Proceedings of the National Academy of Sciences</i>, vol. 118, no. 51, e2113046118, National Academy of Sciences, 2021, doi:<a href=\"https://doi.org/10.1073/pnas.2113046118\">10.1073/pnas.2113046118</a>.","short":"A.J. Johnson, D.A. Dahhan, N. Gnyliukh, W. Kaufmann, V. Zheden, T. Costanzo, P. Mahou, M. Hrtyan, J. Wang, J.L. Aguilera Servin, D. van Damme, E. Beaurepaire, M. Loose, S.Y. Bednarek, J. Friml, Proceedings of the National Academy of Sciences 118 (2021).","ama":"Johnson AJ, Dahhan DA, Gnyliukh N, et al. The TPLATE complex mediates membrane bending during plant clathrin-mediated endocytosis. <i>Proceedings of the National Academy of Sciences</i>. 2021;118(51). doi:<a href=\"https://doi.org/10.1073/pnas.2113046118\">10.1073/pnas.2113046118</a>","ista":"Johnson AJ, Dahhan DA, Gnyliukh N, Kaufmann W, Zheden V, Costanzo T, Mahou P, Hrtyan M, Wang J, Aguilera Servin JL, van Damme D, Beaurepaire E, Loose M, Bednarek SY, Friml J. 2021. The TPLATE complex mediates membrane bending during plant clathrin-mediated endocytosis. Proceedings of the National Academy of Sciences. 118(51), e2113046118.","apa":"Johnson, A. J., Dahhan, D. A., Gnyliukh, N., Kaufmann, W., Zheden, V., Costanzo, T., … Friml, J. (2021). The TPLATE complex mediates membrane bending during plant clathrin-mediated endocytosis. <i>Proceedings of the National Academy of Sciences</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.2113046118\">https://doi.org/10.1073/pnas.2113046118</a>","ieee":"A. J. Johnson <i>et al.</i>, “The TPLATE complex mediates membrane bending during plant clathrin-mediated endocytosis,” <i>Proceedings of the National Academy of Sciences</i>, vol. 118, no. 51. National Academy of Sciences, 2021.","chicago":"Johnson, Alexander J, Dana A Dahhan, Nataliia Gnyliukh, Walter Kaufmann, Vanessa Zheden, Tommaso Costanzo, Pierre Mahou, et al. “The TPLATE Complex Mediates Membrane Bending during Plant Clathrin-Mediated Endocytosis.” <i>Proceedings of the National Academy of Sciences</i>. National Academy of Sciences, 2021. <a href=\"https://doi.org/10.1073/pnas.2113046118\">https://doi.org/10.1073/pnas.2113046118</a>."},"article_processing_charge":"No"},{"title":"Arabidopsis hypocotyl adventitious root formation is suppressed by ABA signaling","acknowledgement":"We thank S. Cutler (Riverside, USA) for providing the ABA biosynthesis mutants and ABA signaling mutants.","year":"2021","department":[{"_id":"JiFr"}],"oa":1,"language":[{"iso":"eng"}],"publication_identifier":{"eissn":["20734425"]},"article_number":"1141","article_type":"original","publication":"Genes","day":"27","doi":"10.3390/genes12081141","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","publication_status":"published","external_id":{"isi":["000690558000001"]},"date_created":"2021-08-15T22:01:28Z","abstract":[{"lang":"eng","text":"Roots are composed of different root types and, in the dicotyledonous Arabidopsis, typically consist of a primary root that branches into lateral roots. Adventitious roots emerge from non-root tissue and are formed upon wounding or other types of abiotic stress. Here, we investigated adventitious root (AR) formation in Arabidopsis hypocotyls under conditions of altered abscisic acid (ABA) signaling. Exogenously applied ABA suppressed AR formation at 0.25 µM or higher doses. AR formation was less sensitive to the synthetic ABA analog pyrabactin (PB). However, PB was a more potent inhibitor at concentrations above 1 µM, suggesting that it was more selective in triggering a root inhibition response. Analysis of a series of phosphonamide and phosphonate pyrabactin analogs suggested that adventitious root formation and lateral root branching are differentially regulated by ABA signaling. ABA biosynthesis and signaling mutants affirmed a general inhibitory role of ABA and point to PYL1 and PYL2 as candidate ABA receptors that regulate AR inhibition."}],"file_date_updated":"2021-08-16T09:02:40Z","scopus_import":"1","intvolume":"        12","date_published":"2021-07-27T00:00:00Z","author":[{"last_name":"Zeng","first_name":"Yinwei","full_name":"Zeng, Yinwei"},{"orcid":"0000-0001-7241-2328","id":"362BF7FE-F248-11E8-B48F-1D18A9856A87","full_name":"Verstraeten, Inge","first_name":"Inge","last_name":"Verstraeten"},{"first_name":"Hoang Khai","full_name":"Trinh, Hoang Khai","last_name":"Trinh"},{"first_name":"Thomas","full_name":"Heugebaert, Thomas","last_name":"Heugebaert"},{"last_name":"Stevens","full_name":"Stevens, Christian V.","first_name":"Christian V."},{"last_name":"Garcia-Maquilon","full_name":"Garcia-Maquilon, Irene","first_name":"Irene"},{"last_name":"Rodriguez","full_name":"Rodriguez, Pedro L.","first_name":"Pedro L."},{"full_name":"Vanneste, Steffen","first_name":"Steffen","last_name":"Vanneste"},{"last_name":"Geelen","first_name":"Danny","full_name":"Geelen, Danny"}],"volume":12,"issue":"8","quality_controlled":"1","_id":"9909","article_processing_charge":"Yes","citation":{"chicago":"Zeng, Yinwei, Inge Verstraeten, Hoang Khai Trinh, Thomas Heugebaert, Christian V. Stevens, Irene Garcia-Maquilon, Pedro L. Rodriguez, Steffen Vanneste, and Danny Geelen. “Arabidopsis Hypocotyl Adventitious Root Formation Is Suppressed by ABA Signaling.” <i>Genes</i>. MDPI, 2021. <a href=\"https://doi.org/10.3390/genes12081141\">https://doi.org/10.3390/genes12081141</a>.","ieee":"Y. Zeng <i>et al.</i>, “Arabidopsis hypocotyl adventitious root formation is suppressed by ABA signaling,” <i>Genes</i>, vol. 12, no. 8. MDPI, 2021.","apa":"Zeng, Y., Verstraeten, I., Trinh, H. K., Heugebaert, T., Stevens, C. V., Garcia-Maquilon, I., … Geelen, D. (2021). Arabidopsis hypocotyl adventitious root formation is suppressed by ABA signaling. <i>Genes</i>. MDPI. <a href=\"https://doi.org/10.3390/genes12081141\">https://doi.org/10.3390/genes12081141</a>","ama":"Zeng Y, Verstraeten I, Trinh HK, et al. Arabidopsis hypocotyl adventitious root formation is suppressed by ABA signaling. <i>Genes</i>. 2021;12(8). doi:<a href=\"https://doi.org/10.3390/genes12081141\">10.3390/genes12081141</a>","ista":"Zeng Y, Verstraeten I, Trinh HK, Heugebaert T, Stevens CV, Garcia-Maquilon I, Rodriguez PL, Vanneste S, Geelen D. 2021. Arabidopsis hypocotyl adventitious root formation is suppressed by ABA signaling. Genes. 12(8), 1141.","mla":"Zeng, Yinwei, et al. “Arabidopsis Hypocotyl Adventitious Root Formation Is Suppressed by ABA Signaling.” <i>Genes</i>, vol. 12, no. 8, 1141, MDPI, 2021, doi:<a href=\"https://doi.org/10.3390/genes12081141\">10.3390/genes12081141</a>.","short":"Y. Zeng, I. Verstraeten, H.K. Trinh, T. Heugebaert, C.V. Stevens, I. Garcia-Maquilon, P.L. Rodriguez, S. Vanneste, D. Geelen, Genes 12 (2021)."},"has_accepted_license":"1","publisher":"MDPI","type":"journal_article","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"file":[{"file_name":"2021_Genes_Zeng.pdf","date_updated":"2021-08-16T09:02:40Z","content_type":"application/pdf","success":1,"file_size":1340305,"relation":"main_file","file_id":"9919","checksum":"3d99535618cf9a5b14d264408fa52e97","creator":"asandaue","access_level":"open_access","date_created":"2021-08-16T09:02:40Z"}],"date_updated":"2023-08-11T10:32:21Z","oa_version":"Published Version","isi":1,"ddc":["580","570"],"month":"07","status":"public"},{"date_created":"2021-09-09T07:37:20Z","publication_status":"published","file_date_updated":"2021-09-15T22:30:26Z","abstract":[{"lang":"eng","text":"Blood – this is what animals use to heal wounds fast and efficient. Plants do not have blood circulation and their cells cannot move. However, plants have evolved remarkable capacities to regenerate tissues and organs preventing further damage. In my PhD research, I studied the wound healing in the Arabidopsis root. I used a UV laser to ablate single cells in the root tip and observed the consequent wound healing. Interestingly, the inner adjacent cells induced a\r\ndivision plane switch and subsequently adopted the cell type of the killed cell to replace it. We termed this form of wound healing “restorative divisions”. This initial observation triggered the questions of my PhD studies: How and why do cells orient their division planes, how do they feel the wound and why does this happen only in inner adjacent cells.\r\nFor answering these questions, I used a quite simple experimental setup: 5 day - old seedlings were stained with propidium iodide to visualize cell walls and dead cells; ablation was carried out using a special laser cutter and a confocal microscope. Adaptation of the novel vertical microscope system made it possible to observe wounds in real time. This revealed that restorative divisions occur at increased frequency compared to normal divisions. Additionally,\r\nthe major plant hormone auxin accumulates in wound adjacent cells and drives the expression of the wound-stress responsive transcription factor ERF115. Using this as a marker gene for wound responses, we found that an important part of wound signalling is the sensing of the collapse of the ablated cell. The collapse causes a radical pressure drop, which results in strong tissue deformations. These deformations manifest in an invasion of the now free spot specifically by the inner adjacent cells within seconds, probably because of higher pressure of the inner tissues. Long-term imaging revealed that those deformed cells continuously expand towards the wound hole and that this is crucial for the restorative division. These wound-expanding cells exhibit an abnormal, biphasic polarity of microtubule arrays\r\nbefore the division. Experiments inhibiting cell expansion suggest that it is the biphasic stretching that induces those MT arrays. Adapting the micromanipulator aspiration system from animal scientists at our institute confirmed the hypothesis that stretching influences microtubule stability. In conclusion, this shows that microtubules react to tissue deformation\r\nand this facilitates the observed division plane switch. This puts mechanical cues and tensions at the most prominent position for explaining the growth and wound healing properties of plants. Hence, it shines light onto the importance of understanding mechanical signal transduction. "}],"_id":"9992","author":[{"id":"2EEE7A2A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8295-2926","first_name":"Lukas","full_name":"Hörmayer, Lukas","last_name":"Hörmayer"}],"page":"168","date_published":"2021-09-13T00:00:00Z","type":"dissertation","article_processing_charge":"No","publisher":"Institute of Science and Technology Austria","has_accepted_license":"1","citation":{"chicago":"Hörmayer, Lukas. “Wound Healing in the Arabidopsis Root Meristem.” Institute of Science and Technology Austria, 2021. <a href=\"https://doi.org/10.15479/at:ista:9992\">https://doi.org/10.15479/at:ista:9992</a>.","apa":"Hörmayer, L. (2021). <i>Wound healing in the Arabidopsis root meristem</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/at:ista:9992\">https://doi.org/10.15479/at:ista:9992</a>","ieee":"L. Hörmayer, “Wound healing in the Arabidopsis root meristem,” Institute of Science and Technology Austria, 2021.","ista":"Hörmayer L. 2021. Wound healing in the Arabidopsis root meristem. Institute of Science and Technology Austria.","ama":"Hörmayer L. Wound healing in the Arabidopsis root meristem. 2021. doi:<a href=\"https://doi.org/10.15479/at:ista:9992\">10.15479/at:ista:9992</a>","mla":"Hörmayer, Lukas. <i>Wound Healing in the Arabidopsis Root Meristem</i>. Institute of Science and Technology Austria, 2021, doi:<a href=\"https://doi.org/10.15479/at:ista:9992\">10.15479/at:ista:9992</a>.","short":"L. Hörmayer, Wound Healing in the Arabidopsis Root Meristem, Institute of Science and Technology Austria, 2021."},"oa_version":"Published Version","date_updated":"2023-09-07T13:38:33Z","month":"09","status":"public","ddc":["575"],"file":[{"file_id":"9993","access_level":"closed","date_created":"2021-09-09T07:29:48Z","creator":"lhoermaye","checksum":"c763064adaa720e16066c1a4f9682bbb","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","date_updated":"2021-09-15T22:30:26Z","file_size":25179004,"embargo_to":"open_access","file_name":"Thesis_vupload.docx","relation":"source_file"},{"date_created":"2021-09-09T14:25:08Z","access_level":"open_access","creator":"lhoermaye","checksum":"53911b06e93d7cdbbf4c7f4c162fa70f","file_id":"9996","relation":"main_file","embargo":"2021-09-09","file_size":6246900,"content_type":"application/pdf","date_updated":"2021-09-15T22:30:26Z","file_name":"Thesis_vfinal_pdfa.pdf"}],"related_material":{"record":[{"status":"public","id":"6351","relation":"part_of_dissertation"},{"id":"6943","relation":"part_of_dissertation","status":"public"},{"status":"public","relation":"part_of_dissertation","id":"8002"}]},"tmp":{"image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"alternative_title":["ISTA Thesis"],"title":"Wound healing in the Arabidopsis root meristem","project":[{"call_identifier":"FWF","_id":"262EF96E-B435-11E9-9278-68D0E5697425","name":"RNA-directed DNA methylation in plant development","grant_number":"P29988"},{"grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","call_identifier":"H2020"}],"department":[{"_id":"GradSch"},{"_id":"JiFr"}],"year":"2021","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"degree_awarded":"PhD","ec_funded":1,"oa":1,"publication_identifier":{"issn":["2663-337X"]},"language":[{"iso":"eng"}],"doi":"10.15479/at:ista:9992","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","day":"13","supervisor":[{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","first_name":"Jiří","full_name":"Friml, Jiří","last_name":"Friml"}]},{"type":"journal_article","publisher":"Elsevier","citation":{"short":"P. He, Y. Zhang, G. Xiao, Molecular Plant 13 (2020) 1238–1240.","mla":"He, Peng, et al. “Origin of a Subgenome and Genome Evolution of Allotetraploid Cotton Species.” <i>Molecular Plant</i>, vol. 13, no. 9, Elsevier, 2020, pp. 1238–40, doi:<a href=\"https://doi.org/10.1016/j.molp.2020.07.006\">10.1016/j.molp.2020.07.006</a>.","ista":"He P, Zhang Y, Xiao G. 2020. Origin of a subgenome and genome evolution of allotetraploid cotton species. Molecular Plant. 13(9), 1238–1240.","ama":"He P, Zhang Y, Xiao G. Origin of a subgenome and genome evolution of allotetraploid cotton species. <i>Molecular Plant</i>. 2020;13(9):1238-1240. doi:<a href=\"https://doi.org/10.1016/j.molp.2020.07.006\">10.1016/j.molp.2020.07.006</a>","apa":"He, P., Zhang, Y., &#38; Xiao, G. (2020). Origin of a subgenome and genome evolution of allotetraploid cotton species. <i>Molecular Plant</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.molp.2020.07.006\">https://doi.org/10.1016/j.molp.2020.07.006</a>","ieee":"P. He, Y. Zhang, and G. Xiao, “Origin of a subgenome and genome evolution of allotetraploid cotton species,” <i>Molecular Plant</i>, vol. 13, no. 9. Elsevier, pp. 1238–1240, 2020.","chicago":"He, Peng, Yuzhou Zhang, and Guanghui Xiao. “Origin of a Subgenome and Genome Evolution of Allotetraploid Cotton Species.” <i>Molecular Plant</i>. Elsevier, 2020. <a href=\"https://doi.org/10.1016/j.molp.2020.07.006\">https://doi.org/10.1016/j.molp.2020.07.006</a>."},"article_processing_charge":"No","status":"public","month":"09","isi":1,"oa_version":"None","date_updated":"2023-08-22T08:40:35Z","scopus_import":"1","date_created":"2020-08-16T22:00:57Z","external_id":{"pmid":["32688032"],"isi":["000566895400007"]},"publication_status":"published","_id":"8271","issue":"9","quality_controlled":"1","volume":13,"page":"1238-1240","author":[{"full_name":"He, Peng","first_name":"Peng","last_name":"He"},{"full_name":"Zhang, Yuzhou","first_name":"Yuzhou","orcid":"0000-0003-2627-6956","id":"3B6137F2-F248-11E8-B48F-1D18A9856A87","last_name":"Zhang"},{"last_name":"Xiao","full_name":"Xiao, Guanghui","first_name":"Guanghui"}],"intvolume":"        13","date_published":"2020-09-07T00:00:00Z","article_type":"original","language":[{"iso":"eng"}],"publication_identifier":{"eissn":["17529867"],"issn":["16742052"]},"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","doi":"10.1016/j.molp.2020.07.006","day":"07","publication":"Molecular Plant","title":"Origin of a subgenome and genome evolution of allotetraploid cotton species","department":[{"_id":"JiFr"}],"year":"2020","acknowledgement":"We thank Dr. Gai Huang for his comments and help. We apologize to authors whose work could not be cited due to space limitation. No conflict of interest declared.","pmid":1},{"department":[{"_id":"JiFr"}],"year":"2020","acknowledgement":"We would like to thank the reviewers for their helpful comments on the original manuscript. ","pmid":1,"title":"AcoMYB4, an Ananas comosus L. MYB transcription factor, functions in osmotic stress through negative regulation of ABA signaling","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","doi":"10.3390/ijms21165727","day":"10","publication":"International Journal of Molecular Sciences","article_type":"original","article_number":"5272","publication_identifier":{"issn":["16616596"],"eissn":["14220067"]},"language":[{"iso":"eng"}],"oa":1,"_id":"8283","issue":"16","quality_controlled":"1","volume":21,"author":[{"last_name":"Chen","full_name":"Chen, Huihuang","first_name":"Huihuang"},{"first_name":"Linyi","full_name":"Lai, Linyi","last_name":"Lai"},{"first_name":"Lanxin","full_name":"Li, Lanxin","id":"367EF8FA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-5607-272X","last_name":"Li"},{"full_name":"Liu, Liping","first_name":"Liping","last_name":"Liu"},{"last_name":"Jakada","first_name":"Bello Hassan","full_name":"Jakada, Bello Hassan"},{"first_name":"Youmei","full_name":"Huang, Youmei","last_name":"Huang"},{"last_name":"He","first_name":"Qing","full_name":"He, Qing"},{"first_name":"Mengnan","full_name":"Chai, Mengnan","last_name":"Chai"},{"full_name":"Niu, Xiaoping","first_name":"Xiaoping","last_name":"Niu"},{"last_name":"Qin","first_name":"Yuan","full_name":"Qin, Yuan"}],"date_published":"2020-08-10T00:00:00Z","intvolume":"        21","scopus_import":"1","file_date_updated":"2020-08-25T09:53:50Z","abstract":[{"text":"Drought and salt stress are the main environmental cues affecting the survival, development, distribution, and yield of crops worldwide. MYB transcription factors play a crucial role in plants’ biological processes, but the function of pineapple MYB genes is still obscure. In this study, one of the pineapple MYB transcription factors, AcoMYB4, was isolated and characterized. The results showed that AcoMYB4 is localized in the cell nucleus, and its expression is induced by low temperature, drought, salt stress, and hormonal stimulation, especially by abscisic acid (ABA). Overexpression of AcoMYB4 in rice and Arabidopsis enhanced plant sensitivity to osmotic stress; it led to an increase in the number stomata on leaf surfaces and lower germination rate under salt and drought stress. Furthermore, in AcoMYB4 OE lines, the membrane oxidation index, free proline, and soluble sugar contents were decreased. In contrast, electrolyte leakage and malondialdehyde (MDA) content increased significantly due to membrane injury, indicating higher sensitivity to drought and salinity stresses. Besides the above, both the expression level and activities of several antioxidant enzymes were decreased, indicating lower antioxidant activity in AcoMYB4 transgenic plants. Moreover, under osmotic stress, overexpression of AcoMYB4 inhibited ABA biosynthesis through a decrease in the transcription of genes responsible for ABA synthesis (ABA1 and ABA2) and ABA signal transduction factor ABI5. These results suggest that AcoMYB4 negatively regulates osmotic stress by attenuating cellular ABA biosynthesis and signal transduction pathways. ","lang":"eng"}],"date_created":"2020-08-24T06:24:03Z","publication_status":"published","external_id":{"pmid":["32785037"],"isi":["000565090300001"]},"month":"08","status":"public","ddc":["570"],"isi":1,"oa_version":"Published Version","date_updated":"2024-10-29T10:22:43Z","file":[{"content_type":"application/pdf","date_updated":"2020-08-25T09:53:50Z","file_size":5718755,"success":1,"file_name":"2020_IntMolecSciences_Chen.pdf","relation":"main_file","file_id":"8292","access_level":"open_access","date_created":"2020-08-25T09:53:50Z","checksum":"03b039244e6ae80580385fd9f577e2b2","creator":"cziletti"}],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"10083"}]},"type":"journal_article","publisher":"MDPI","has_accepted_license":"1","citation":{"ista":"Chen H, Lai L, Li L, Liu L, Jakada BH, Huang Y, He Q, Chai M, Niu X, Qin Y. 2020. AcoMYB4, an Ananas comosus L. MYB transcription factor, functions in osmotic stress through negative regulation of ABA signaling. International Journal of Molecular Sciences. 21(16), 5272.","ama":"Chen H, Lai L, Li L, et al. AcoMYB4, an Ananas comosus L. MYB transcription factor, functions in osmotic stress through negative regulation of ABA signaling. <i>International Journal of Molecular Sciences</i>. 2020;21(16). doi:<a href=\"https://doi.org/10.3390/ijms21165727\">10.3390/ijms21165727</a>","mla":"Chen, Huihuang, et al. “AcoMYB4, an Ananas Comosus L. MYB Transcription Factor, Functions in Osmotic Stress through Negative Regulation of ABA Signaling.” <i>International Journal of Molecular Sciences</i>, vol. 21, no. 16, 5272, MDPI, 2020, doi:<a href=\"https://doi.org/10.3390/ijms21165727\">10.3390/ijms21165727</a>.","short":"H. Chen, L. Lai, L. Li, L. Liu, B.H. Jakada, Y. Huang, Q. He, M. Chai, X. Niu, Y. Qin, International Journal of Molecular Sciences 21 (2020).","chicago":"Chen, Huihuang, Linyi Lai, Lanxin Li, Liping Liu, Bello Hassan Jakada, Youmei Huang, Qing He, Mengnan Chai, Xiaoping Niu, and Yuan Qin. “AcoMYB4, an Ananas Comosus L. MYB Transcription Factor, Functions in Osmotic Stress through Negative Regulation of ABA Signaling.” <i>International Journal of Molecular Sciences</i>. MDPI, 2020. <a href=\"https://doi.org/10.3390/ijms21165727\">https://doi.org/10.3390/ijms21165727</a>.","ieee":"H. Chen <i>et al.</i>, “AcoMYB4, an Ananas comosus L. MYB transcription factor, functions in osmotic stress through negative regulation of ABA signaling,” <i>International Journal of Molecular Sciences</i>, vol. 21, no. 16. MDPI, 2020.","apa":"Chen, H., Lai, L., Li, L., Liu, L., Jakada, B. H., Huang, Y., … Qin, Y. (2020). AcoMYB4, an Ananas comosus L. MYB transcription factor, functions in osmotic stress through negative regulation of ABA signaling. <i>International Journal of Molecular Sciences</i>. MDPI. <a href=\"https://doi.org/10.3390/ijms21165727\">https://doi.org/10.3390/ijms21165727</a>"},"article_processing_charge":"No"},{"title":"Cell-surface receptors enable perception of extracellular cytokinins","acknowledgement":"We thank Bruno Müller and Aaron Rashotte for critical discussions and provision of plant lines used in this work, Roger Granbom and Tamara Hernández Verdeja (UPSC, Umeå, Sweden) for technical assistance and providing materials, Zuzana Pěkná and Karolina Wojewodová (CRH, Palacký University, Olomouc, Czech Republic) for help with cytokinin receptor binding assays, and David Zalabák (CRH, Palacký University, Olomouc, Czech Republic) for provision of vector pINIIIΔEH expressing CRE1/AHK4. The bioimaging facility of IST Austria, the Swedish Metabolomics Centre and the IST Austria Bio-Imaging facility are acknowledged for support. The work was funded by the European Molecular Biology Organization (EMBO ASTF 297-2013) (I.A.), Development—The Company of Biologists (DEVTF2012) (I.A.; C.T.), Plant Fellows (the International Post doc Fellowship Programme in Plant Sciences, 267423) (I.A.; K.L.), the Swedish Research Council (621-2014-4514) (K.L.), UPSC Berzelii Center for Forest Biotechnology (Vinnova 2012-01560), Kempestiftelserna (JCK-2711) (K.L.) and (JCK-1811) (E.-M.B., K.L.). The Ministry of Education, Youth and Sports of the Czech Republic via the European Regional Development Fund-Project “Plants as a tool for sustainable global development” (CZ.02.1.01/0.0/0.0/16_019/0000827) (O.N., O.P., R.S., V.M., L.P., K.D.) and project CEITEC 2020 (LQ1601) (M.P., J.H.) provided support, as did the Czech Science Foundation via projects GP14-30004P (M.P.) and 16-04184S (O.P., K.D., O.N.), Vetenskapsrådet and Vinnova (Verket för Innovationssystem) (T.V., S.R.), Knut och Alice Wallenbergs Stiftelse via “Shapesystem” grant number 2012.0050. A.J. was supported by the Austria Science Fund (FWF): I03630 to J.F. The research leading to these results received funding from European Union’s Horizon 2020 programme (ERC grant no. 742985) and FWO-FWF joint project G0E5718N to J.F.","project":[{"grant_number":"I03630","name":"Molecular mechanisms of endocytic cargo recognition in plants","_id":"26538374-B435-11E9-9278-68D0E5697425","call_identifier":"FWF"},{"name":"Tracing Evolution of Auxin Transport and Polarity in Plants","_id":"261099A6-B435-11E9-9278-68D0E5697425","grant_number":"742985","call_identifier":"H2020"}],"department":[{"_id":"JiFr"}],"year":"2020","acknowledged_ssus":[{"_id":"Bio"}],"article_number":"4284","article_type":"original","ec_funded":1,"oa":1,"publication_identifier":{"eissn":["20411723"]},"language":[{"iso":"eng"}],"doi":"10.1038/s41467-020-17700-9","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","day":"27","publication":"Nature Communications","scopus_import":"1","date_created":"2020-09-06T22:01:13Z","publication_status":"published","external_id":{"isi":["000567931000001"]},"file_date_updated":"2020-12-10T12:23:56Z","abstract":[{"lang":"eng","text":"Cytokinins are mobile multifunctional plant hormones with roles in development and stress resilience. Although their Histidine Kinase receptors are substantially localised to the endoplasmic reticulum, cellular sites of cytokinin perception and importance of spatially heterogeneous cytokinin distribution continue to be debated. Here we show that cytokinin perception by plasma membrane receptors is an effective additional path for cytokinin response. Readout from a Two Component Signalling cytokinin-specific reporter (TCSn::GFP) closely matches intracellular cytokinin content in roots, yet we also find cytokinins in extracellular fluid, potentially enabling action at the cell surface. Cytokinins covalently linked to beads that could not pass the plasma membrane increased expression of both TCSn::GFP and Cytokinin Response Factors. Super-resolution microscopy of GFP-labelled receptors and diminished TCSn::GFP response to immobilised cytokinins in cytokinin receptor mutants, further indicate that receptors can function at the cell surface. We argue that dual intracellular and surface locations may augment flexibility of cytokinin responses."}],"quality_controlled":"1","_id":"8337","volume":11,"author":[{"first_name":"Ioanna","full_name":"Antoniadi, Ioanna","last_name":"Antoniadi"},{"first_name":"Ondřej","full_name":"Novák, Ondřej","last_name":"Novák"},{"id":"0AE74790-0E0B-11E9-ABC7-1ACFE5697425","orcid":"0000-0003-4783-1752","full_name":"Gelová, Zuzana","first_name":"Zuzana","last_name":"Gelová"},{"full_name":"Johnson, Alexander J","first_name":"Alexander J","id":"46A62C3A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-2739-8843","last_name":"Johnson"},{"full_name":"Plíhal, Ondřej","first_name":"Ondřej","last_name":"Plíhal"},{"full_name":"Simerský, Radim","first_name":"Radim","last_name":"Simerský"},{"first_name":"Václav","full_name":"Mik, Václav","last_name":"Mik"},{"last_name":"Vain","first_name":"Thomas","full_name":"Vain, Thomas"},{"last_name":"Mateo-Bonmatí","first_name":"Eduardo","full_name":"Mateo-Bonmatí, Eduardo"},{"first_name":"Michal","full_name":"Karady, Michal","last_name":"Karady"},{"full_name":"Pernisová, Markéta","first_name":"Markéta","last_name":"Pernisová"},{"full_name":"Plačková, Lenka","first_name":"Lenka","last_name":"Plačková"},{"last_name":"Opassathian","first_name":"Korawit","full_name":"Opassathian, Korawit"},{"last_name":"Hejátko","first_name":"Jan","full_name":"Hejátko, Jan"},{"last_name":"Robert","full_name":"Robert, Stéphanie","first_name":"Stéphanie"},{"last_name":"Friml","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Doležal, Karel","first_name":"Karel","last_name":"Doležal"},{"last_name":"Ljung","first_name":"Karin","full_name":"Ljung, Karin"},{"last_name":"Turnbull","full_name":"Turnbull, Colin","first_name":"Colin"}],"intvolume":"        11","date_published":"2020-08-27T00:00:00Z","type":"journal_article","article_processing_charge":"No","publisher":"Springer Nature","has_accepted_license":"1","citation":{"chicago":"Antoniadi, Ioanna, Ondřej Novák, Zuzana Gelová, Alexander J Johnson, Ondřej Plíhal, Radim Simerský, Václav Mik, et al. “Cell-Surface Receptors Enable Perception of Extracellular Cytokinins.” <i>Nature Communications</i>. Springer Nature, 2020. <a href=\"https://doi.org/10.1038/s41467-020-17700-9\">https://doi.org/10.1038/s41467-020-17700-9</a>.","ieee":"I. Antoniadi <i>et al.</i>, “Cell-surface receptors enable perception of extracellular cytokinins,” <i>Nature Communications</i>, vol. 11. Springer Nature, 2020.","apa":"Antoniadi, I., Novák, O., Gelová, Z., Johnson, A. J., Plíhal, O., Simerský, R., … Turnbull, C. (2020). Cell-surface receptors enable perception of extracellular cytokinins. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-020-17700-9\">https://doi.org/10.1038/s41467-020-17700-9</a>","ama":"Antoniadi I, Novák O, Gelová Z, et al. Cell-surface receptors enable perception of extracellular cytokinins. <i>Nature Communications</i>. 2020;11. doi:<a href=\"https://doi.org/10.1038/s41467-020-17700-9\">10.1038/s41467-020-17700-9</a>","ista":"Antoniadi I, Novák O, Gelová Z, Johnson AJ, Plíhal O, Simerský R, Mik V, Vain T, Mateo-Bonmatí E, Karady M, Pernisová M, Plačková L, Opassathian K, Hejátko J, Robert S, Friml J, Doležal K, Ljung K, Turnbull C. 2020. Cell-surface receptors enable perception of extracellular cytokinins. Nature Communications. 11, 4284.","short":"I. Antoniadi, O. Novák, Z. Gelová, A.J. Johnson, O. Plíhal, R. Simerský, V. Mik, T. Vain, E. Mateo-Bonmatí, M. Karady, M. Pernisová, L. Plačková, K. Opassathian, J. Hejátko, S. Robert, J. Friml, K. Doležal, K. Ljung, C. Turnbull, Nature Communications 11 (2020).","mla":"Antoniadi, Ioanna, et al. “Cell-Surface Receptors Enable Perception of Extracellular Cytokinins.” <i>Nature Communications</i>, vol. 11, 4284, Springer Nature, 2020, doi:<a href=\"https://doi.org/10.1038/s41467-020-17700-9\">10.1038/s41467-020-17700-9</a>."},"oa_version":"Published Version","date_updated":"2023-08-22T09:10:32Z","month":"08","status":"public","isi":1,"ddc":["580"],"file":[{"file_id":"8936","creator":"dernst","checksum":"5b96f39b598de7510cfefefb819b9a6d","date_created":"2020-12-10T12:23:56Z","access_level":"open_access","file_name":"2020_NatureComm_Antoniadi.pdf","success":1,"file_size":3526415,"date_updated":"2020-12-10T12:23:56Z","content_type":"application/pdf","relation":"main_file"}],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"}},{"date_published":"2020-09-30T00:00:00Z","page":"164","author":[{"id":"31435098-F248-11E8-B48F-1D18A9856A87","first_name":"Huibin","full_name":"Han, Huibin","last_name":"Han"}],"_id":"8589","date_created":"2020-09-30T14:50:51Z","publication_status":"published","file_date_updated":"2021-10-01T13:33:02Z","abstract":[{"text":"The plant hormone auxin plays indispensable roles in plant growth and development. An essential level of regulation in auxin action is the directional auxin transport within cells. The establishment of auxin gradient in plant tissue has been attributed to local auxin biosynthesis and directional intercellular auxin transport, which both are controlled by various environmental and developmental signals. It is well established that asymmetric auxin distribution in cells is achieved by polarly localized PIN-FORMED (PIN) auxin efflux transporters. Despite the initial insights into cellular mechanisms of PIN polarization obtained from the last decades, the molecular mechanism and specific regulators mediating PIN polarization remains elusive. In this thesis, we aim to find novel players in PIN subcellular polarity regulation during Arabidopsis development. We first characterize the physiological effect of piperonylic acid (PA) on Arabidopsis hypocotyl gravitropic bending and PIN polarization. Secondly, we reveal the importance of SCFTIR1/AFB auxin signaling pathway in shoot gravitropism bending termination. In addition, we also explore the role of myosin XI complex, and actin cytoskeleton in auxin feedback regulation on PIN polarity. In Chapter 1, we give an overview of the current knowledge about PIN-mediated auxin fluxes in various plant tropic responses. In Chapter 2, we study the physiological effect of PA on shoot gravitropic bending. Our results show that PA treatment inhibits auxin-mediated PIN3 repolarization by interfering with PINOID and PIN3 phosphorylation status, ultimately leading to hyperbending hypocotyls. In Chapter 3, we provide evidence to show that the SCFTIR1/AFB nuclear auxin signaling pathway is crucial and required for auxin-mediated PIN3 repolarization and shoot gravitropic bending termination. In Chapter 4, we perform a phosphoproteomics approach and identify the motor protein Myosin XI and its binding protein, the MadB2 family, as an essential regulator of PIN polarity for auxin-canalization related developmental processes. In Chapter 5, we demonstrate the vital role of actin cytoskeleton in auxin feedback on PIN polarity by regulating PIN subcellular trafficking. Overall, the data presented in this PhD thesis brings novel insights into the PIN polar localization regulation that resulted in the (re)establishment of the polar auxin flow and gradient in response to environmental stimuli during plant development.","lang":"eng"}],"related_material":{"record":[{"id":"7643","relation":"part_of_dissertation","status":"public"}]},"file":[{"file_id":"8590","creator":"dernst","checksum":"c4bda1947d4c09c428ac9ce667b02327","access_level":"closed","date_created":"2020-09-30T14:50:20Z","file_name":"2020_Han_Thesis.docx","date_updated":"2020-09-30T14:50:20Z","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","file_size":49198118,"relation":"source_file"},{"file_id":"8591","checksum":"3f4f5d1718c2230adf30639ecaf8a00b","creator":"dernst","date_created":"2020-09-30T14:49:59Z","access_level":"open_access","file_name":"2020_Han_Thesis.pdf","file_size":15513963,"date_updated":"2021-10-01T13:33:02Z","content_type":"application/pdf","relation":"main_file"}],"date_updated":"2023-09-07T13:13:05Z","oa_version":"Published Version","ddc":["580"],"status":"public","month":"09","article_processing_charge":"No","citation":{"ama":"Han H. Novel insights into PIN polarity regulation during Arabidopsis development. 2020. doi:<a href=\"https://doi.org/10.15479/AT:ISTA:8589\">10.15479/AT:ISTA:8589</a>","ista":"Han H. 2020. Novel insights into PIN polarity regulation during Arabidopsis development. Institute of Science and Technology Austria.","mla":"Han, Huibin. <i>Novel Insights into PIN Polarity Regulation during Arabidopsis Development</i>. Institute of Science and Technology Austria, 2020, doi:<a href=\"https://doi.org/10.15479/AT:ISTA:8589\">10.15479/AT:ISTA:8589</a>.","short":"H. Han, Novel Insights into PIN Polarity Regulation during Arabidopsis Development, Institute of Science and Technology Austria, 2020.","chicago":"Han, Huibin. “Novel Insights into PIN Polarity Regulation during Arabidopsis Development.” Institute of Science and Technology Austria, 2020. <a href=\"https://doi.org/10.15479/AT:ISTA:8589\">https://doi.org/10.15479/AT:ISTA:8589</a>.","apa":"Han, H. (2020). <i>Novel insights into PIN polarity regulation during Arabidopsis development</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT:ISTA:8589\">https://doi.org/10.15479/AT:ISTA:8589</a>","ieee":"H. Han, “Novel insights into PIN polarity regulation during Arabidopsis development,” Institute of Science and Technology Austria, 2020."},"has_accepted_license":"1","publisher":"Institute of Science and Technology Austria","type":"dissertation","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"acknowledgement":"I also want to thank the China Scholarship Council for supporting my study during the year from 2015 to 2019. I also want to thank IST facilities – the Bioimaging facility, the media kitchen, the plant facility and all of the campus services, for their support.","year":"2020","department":[{"_id":"JiFr"}],"alternative_title":["ISTA Thesis"],"title":"Novel insights into PIN polarity regulation during Arabidopsis development","day":"30","supervisor":[{"last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","first_name":"Jiří","full_name":"Friml, Jiří"}],"doi":"10.15479/AT:ISTA:8589","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","oa":1,"publication_identifier":{"issn":["2663-337X"]},"language":[{"iso":"eng"}],"degree_awarded":"PhD"},{"date_updated":"2023-09-05T12:21:32Z","oa_version":"Published Version","isi":1,"status":"public","month":"11","type":"journal_article","article_processing_charge":"No","citation":{"ieee":"D. Liu <i>et al.</i>, “Endocytosis of BRASSINOSTEROID INSENSITIVE1 is partly driven by a canonical tyrosine-based Motif,” <i>Plant Cell</i>, vol. 32, no. 11. American Society of Plant Biologists, pp. 3598–3612, 2020.","apa":"Liu, D., Kumar, R., LAN, C., Johnson, A. J., Siao, W., Vanhoutte, I., … Russinova, E. (2020). Endocytosis of BRASSINOSTEROID INSENSITIVE1 is partly driven by a canonical tyrosine-based Motif. <i>Plant Cell</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1105/tpc.20.00384\">https://doi.org/10.1105/tpc.20.00384</a>","chicago":"Liu, D, R Kumar, Claus LAN, Alexander J Johnson, W Siao, I Vanhoutte, P Wang, et al. “Endocytosis of BRASSINOSTEROID INSENSITIVE1 Is Partly Driven by a Canonical Tyrosine-Based Motif.” <i>Plant Cell</i>. American Society of Plant Biologists, 2020. <a href=\"https://doi.org/10.1105/tpc.20.00384\">https://doi.org/10.1105/tpc.20.00384</a>.","short":"D. Liu, R. Kumar, C. LAN, A.J. Johnson, W. Siao, I. Vanhoutte, P. Wang, K. Bender, K. Yperman, S. Martins, X. Zhao, G. Vert, D. Van Damme, J. Friml, E. Russinova, Plant Cell 32 (2020) 3598–3612.","mla":"Liu, D., et al. “Endocytosis of BRASSINOSTEROID INSENSITIVE1 Is Partly Driven by a Canonical Tyrosine-Based Motif.” <i>Plant Cell</i>, vol. 32, no. 11, American Society of Plant Biologists, 2020, pp. 3598–612, doi:<a href=\"https://doi.org/10.1105/tpc.20.00384\">10.1105/tpc.20.00384</a>.","ama":"Liu D, Kumar R, LAN C, et al. Endocytosis of BRASSINOSTEROID INSENSITIVE1 is partly driven by a canonical tyrosine-based Motif. <i>Plant Cell</i>. 2020;32(11):3598-3612. doi:<a href=\"https://doi.org/10.1105/tpc.20.00384\">10.1105/tpc.20.00384</a>","ista":"Liu D, Kumar R, LAN C, Johnson AJ, Siao W, Vanhoutte I, Wang P, Bender K, Yperman K, Martins S, Zhao X, Vert G, Van Damme D, Friml J, Russinova E. 2020. Endocytosis of BRASSINOSTEROID INSENSITIVE1 is partly driven by a canonical tyrosine-based Motif. Plant Cell. 32(11), 3598–3612."},"publisher":"American Society of Plant Biologists","issue":"11","quality_controlled":"1","_id":"8607","date_published":"2020-11-01T00:00:00Z","intvolume":"        32","author":[{"first_name":"D","full_name":"Liu, D","last_name":"Liu"},{"full_name":"Kumar, R","first_name":"R","last_name":"Kumar"},{"first_name":"Claus","full_name":"LAN, Claus","last_name":"LAN"},{"orcid":"0000-0002-2739-8843","full_name":"Johnson, Alexander J","id":"46A62C3A-F248-11E8-B48F-1D18A9856A87","first_name":"Alexander J","last_name":"Johnson"},{"full_name":"Siao, W","first_name":"W","last_name":"Siao"},{"first_name":"I","full_name":"Vanhoutte, I","last_name":"Vanhoutte"},{"last_name":"Wang","full_name":"Wang, P","first_name":"P"},{"last_name":"Bender","first_name":"KW","full_name":"Bender, KW"},{"last_name":"Yperman","full_name":"Yperman, K","first_name":"K"},{"first_name":"S","full_name":"Martins, S","last_name":"Martins"},{"first_name":"X","full_name":"Zhao, X","last_name":"Zhao"},{"first_name":"G","full_name":"Vert, G","last_name":"Vert"},{"last_name":"Van Damme","full_name":"Van Damme, D","first_name":"D"},{"last_name":"Friml","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","orcid":"0000-0002-8302-7596"},{"full_name":"Russinova, E","first_name":"E","last_name":"Russinova"}],"page":"3598-3612","volume":32,"scopus_import":"1","date_created":"2020-10-05T12:45:16Z","publication_status":"published","external_id":{"pmid":["32958564"],"isi":["000600226800021"]},"abstract":[{"lang":"eng","text":"Clathrin-mediated endocytosis (CME) and its core endocytic machinery are evolutionarily conserved across all eukaryotes. In mammals, the heterotetrameric adaptor protein complex-2 (AP-2) sorts plasma membrane (PM) cargoes into vesicles through the recognition of motifs based on tyrosine or di-leucine in their cytoplasmic tails. However, in plants, very little is known on how PM proteins are sorted for CME and whether similar motifs are required. In Arabidopsis thaliana, the brassinosteroid (BR) receptor, BR INSENSITIVE1 (BRI1), undergoes endocytosis that depends on clathrin and AP-2. Here we demonstrate that BRI1 binds directly to the medium AP-2 subunit, AP2M. The cytoplasmic domain of BRI1 contains five putative canonical surface-exposed tyrosine-based endocytic motifs. The tyrosine-to-phenylalanine substitution in Y898KAI reduced BRI1 internalization without affecting its kinase activity. Consistently, plants carrying the BRI1Y898F mutation were hypersensitive to BRs. Our study demonstrates that AP-2-dependent internalization of PM proteins via the recognition of functional tyrosine motifs also operates in plants."}],"doi":"10.1105/tpc.20.00384","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","publication":"Plant Cell","day":"01","article_type":"original","ec_funded":1,"oa":1,"publication_identifier":{"issn":["1040-4651"],"eissn":["1532-298x"]},"language":[{"iso":"eng"}],"project":[{"grant_number":"I03630","_id":"26538374-B435-11E9-9278-68D0E5697425","name":"Molecular mechanisms of endocytic cargo recognition in plants","call_identifier":"FWF"},{"call_identifier":"H2020","grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425","name":"Tracing Evolution of Auxin Transport and Polarity in Plants"}],"year":"2020","department":[{"_id":"JiFr"}],"pmid":1,"main_file_link":[{"open_access":"1","url":"https://europepmc.org/article/MED/32958564"}],"title":"Endocytosis of BRASSINOSTEROID INSENSITIVE1 is partly driven by a canonical tyrosine-based Motif"},{"publisher":"American Association for the Advancement of Science","citation":{"ama":"Hajny J, Prat T, Rydza N, et al. Receptor kinase module targets PIN-dependent auxin transport during canalization. <i>Science</i>. 2020;370(6516):550-557. doi:<a href=\"https://doi.org/10.1126/science.aba3178\">10.1126/science.aba3178</a>","ista":"Hajny J, Prat T, Rydza N, Rodriguez Solovey L, Tan S, Verstraeten I, Domjan D, Mazur E, Smakowska-Luzan E, Smet W, Mor E, Nolf J, Yang B, Grunewald W, Molnar G, Belkhadir Y, De Rybel B, Friml J. 2020. Receptor kinase module targets PIN-dependent auxin transport during canalization. Science. 370(6516), 550–557.","short":"J. Hajny, T. Prat, N. Rydza, L. Rodriguez Solovey, S. Tan, I. Verstraeten, D. Domjan, E. Mazur, E. Smakowska-Luzan, W. Smet, E. Mor, J. Nolf, B. Yang, W. Grunewald, G. Molnar, Y. Belkhadir, B. De Rybel, J. Friml, Science 370 (2020) 550–557.","mla":"Hajny, Jakub, et al. “Receptor Kinase Module Targets PIN-Dependent Auxin Transport during Canalization.” <i>Science</i>, vol. 370, no. 6516, American Association for the Advancement of Science, 2020, pp. 550–57, doi:<a href=\"https://doi.org/10.1126/science.aba3178\">10.1126/science.aba3178</a>.","chicago":"Hajny, Jakub, Tomas Prat, N Rydza, Lesia Rodriguez Solovey, Shutang Tan, Inge Verstraeten, David Domjan, et al. “Receptor Kinase Module Targets PIN-Dependent Auxin Transport during Canalization.” <i>Science</i>. American Association for the Advancement of Science, 2020. <a href=\"https://doi.org/10.1126/science.aba3178\">https://doi.org/10.1126/science.aba3178</a>.","ieee":"J. Hajny <i>et al.</i>, “Receptor kinase module targets PIN-dependent auxin transport during canalization,” <i>Science</i>, vol. 370, no. 6516. American Association for the Advancement of Science, pp. 550–557, 2020.","apa":"Hajny, J., Prat, T., Rydza, N., Rodriguez Solovey, L., Tan, S., Verstraeten, I., … Friml, J. (2020). Receptor kinase module targets PIN-dependent auxin transport during canalization. <i>Science</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/science.aba3178\">https://doi.org/10.1126/science.aba3178</a>"},"article_processing_charge":"No","type":"journal_article","related_material":{"link":[{"description":"News on IST Homepage","url":"https://ist.ac.at/en/news/molecular-compass-for-cell-orientation/","relation":"press_release"}]},"status":"public","month":"10","isi":1,"oa_version":"Published Version","date_updated":"2023-09-05T12:02:35Z","abstract":[{"lang":"eng","text":"Spontaneously arising channels that transport the phytohormone auxin provide positional cues for self-organizing aspects of plant development such as flexible vasculature regeneration or its patterning during leaf venation. The auxin canalization hypothesis proposes a feedback between auxin signaling and transport as the underlying mechanism, but molecular players await discovery. We identified part of the machinery that routes auxin transport. The auxin-regulated receptor CAMEL (Canalization-related Auxin-regulated Malectin-type RLK) together with CANAR (Canalization-related Receptor-like kinase) interact with and phosphorylate PIN auxin transporters. camel and canar mutants are impaired in PIN1 subcellular trafficking and auxin-mediated PIN polarization, which macroscopically manifests as defects in leaf venation and vasculature regeneration after wounding. The CAMEL-CANAR receptor complex is part of the auxin feedback that coordinates polarization of individual cells during auxin canalization."}],"publication_status":"published","date_created":"2020-11-02T10:04:46Z","external_id":{"pmid":["33122378"],"isi":["000583031800041"]},"scopus_import":"1","volume":370,"page":"550-557","author":[{"last_name":"Hajny","first_name":"Jakub","full_name":"Hajny, Jakub","orcid":"0000-0003-2140-7195","id":"4800CC20-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Prat","id":"3DA3BFEE-F248-11E8-B48F-1D18A9856A87","full_name":"Prat, Tomas","first_name":"Tomas"},{"first_name":"N","full_name":"Rydza, N","last_name":"Rydza"},{"orcid":"0000-0002-7244-7237","full_name":"Rodriguez Solovey, Lesia","id":"3922B506-F248-11E8-B48F-1D18A9856A87","first_name":"Lesia","last_name":"Rodriguez Solovey"},{"last_name":"Tan","full_name":"Tan, Shutang","first_name":"Shutang","id":"2DE75584-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0471-8285"},{"first_name":"Inge","full_name":"Verstraeten, Inge","orcid":"0000-0001-7241-2328","id":"362BF7FE-F248-11E8-B48F-1D18A9856A87","last_name":"Verstraeten"},{"last_name":"Domjan","full_name":"Domjan, David","first_name":"David","id":"C684CD7A-257E-11EA-9B6F-D8588B4F947F","orcid":"0000-0003-2267-106X"},{"full_name":"Mazur, E","first_name":"E","last_name":"Mazur"},{"first_name":"E","full_name":"Smakowska-Luzan, E","last_name":"Smakowska-Luzan"},{"full_name":"Smet, W","first_name":"W","last_name":"Smet"},{"last_name":"Mor","first_name":"E","full_name":"Mor, E"},{"last_name":"Nolf","full_name":"Nolf, J","first_name":"J"},{"last_name":"Yang","full_name":"Yang, B","first_name":"B"},{"last_name":"Grunewald","full_name":"Grunewald, W","first_name":"W"},{"last_name":"Molnar","full_name":"Molnar, Gergely","id":"34F1AF46-F248-11E8-B48F-1D18A9856A87","first_name":"Gergely"},{"first_name":"Y","full_name":"Belkhadir, Y","last_name":"Belkhadir"},{"full_name":"De Rybel, B","first_name":"B","last_name":"De Rybel"},{"first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří","last_name":"Friml"}],"date_published":"2020-10-30T00:00:00Z","intvolume":"       370","_id":"8721","quality_controlled":"1","issue":"6516","publication_identifier":{"issn":["0036-8075"],"eissn":["1095-9203"]},"language":[{"iso":"eng"}],"oa":1,"ec_funded":1,"article_type":"original","day":"30","publication":"Science","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","doi":"10.1126/science.aba3178","title":"Receptor kinase module targets PIN-dependent auxin transport during canalization","main_file_link":[{"url":"https://europepmc.org/article/MED/33122378#free-full-text","open_access":"1"}],"pmid":1,"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"department":[{"_id":"JiFr"}],"year":"2020","acknowledgement":"We acknowledge M. Glanc and Y. Zhang for providing entryclones; Vienna Biocenter Core Facilities (VBCF) for recombinantprotein production and purification; Vienna Biocenter Massspectrometry Facility, Bioimaging, and Life Science Facilities at IST Austria and Proteomics Core Facility CEITEC for a great assistance.Funding:This project received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant agreement 742985) and Austrian Science Fund (FWF): I 3630-B25 to J.F.and by grants from the Austrian Academy of Science through the Gregor Mendel Institute (Y.B.) and the Austrian Agency for International Cooperation in Education and Research (D.D.); the Netherlands Organization for Scientific Research (NWO; VIDI-864.13.001) (W.S.); the Research Foundation–Flanders (FWO;Odysseus II G0D0515N) and a European Research Council grant (ERC; StG TORPEDO; 714055) to B.D.R., B.Y., and E.M.; and the Hertha Firnberg Programme postdoctoral fellowship (T-947) from the FWF Austrian Science Fund to E.S.-L.; J.H. is the recipient of a DOC Fellowship of the Austrian Academy of Sciences at IST Austria.","project":[{"grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","call_identifier":"H2020"},{"grant_number":"I03630","name":"Molecular mechanisms of endocytic cargo recognition in plants","_id":"26538374-B435-11E9-9278-68D0E5697425","call_identifier":"FWF"},{"grant_number":"25239","name":"Cell surface receptor complexes for PIN polarity and auxin-mediated development","_id":"2699E3D2-B435-11E9-9278-68D0E5697425"}]},{"year":"2020","department":[{"_id":"JiFr"}],"title":"Identification and characterization of the molecular machinery of auxin-dependent canalization during vasculature formation and regeneration","alternative_title":["ISTA Thesis"],"supervisor":[{"full_name":"Friml, Jiří","first_name":"Jiří","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml"}],"day":"01","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","doi":"10.15479/AT:ISTA:8822","language":[{"iso":"eng"}],"publication_identifier":{"issn":["2663-337X"]},"oa":1,"degree_awarded":"PhD","date_published":"2020-12-01T00:00:00Z","author":[{"id":"4800CC20-F248-11E8-B48F-1D18A9856A87","full_name":"Hajny, Jakub","first_name":"Jakub","orcid":"0000-0003-2140-7195","last_name":"Hajny"}],"page":"249","_id":"8822","abstract":[{"lang":"eng","text":"Self-organization is a hallmark of plant development manifested e.g. by intricate leaf vein patterns, flexible formation of vasculature during organogenesis or its regeneration following wounding. Spontaneously arising channels transporting the phytohormone auxin, created by coordinated polar localizations of PIN-FORMED 1 (PIN1) auxin exporter, provide positional cues for these as well as other plant patterning processes. To find regulators acting downstream of auxin and the TIR1/AFB auxin signaling pathway essential for PIN1 coordinated polarization during auxin canalization, we performed microarray experiments. Besides the known components of general PIN polarity maintenance, such as PID and PIP5K kinases, we identified and characterized a new regulator of auxin canalization, the transcription factor WRKY DNA-BINDING PROTEIN 23 (WRKY23).\r\nNext, we designed a subsequent microarray experiment to further uncover other molecular players, downstream of auxin-TIR1/AFB-WRKY23 involved in the regulation of auxin-mediated PIN repolarization. We identified a novel and crucial part of the molecular machinery underlying auxin canalization. The auxin-regulated malectin-type receptor-like kinase CAMEL and the associated leucine-rich repeat receptor-like kinase CANAR target and directly phosphorylate PIN auxin transporters. camel and canar mutants are impaired in PIN1 subcellular trafficking and auxin-mediated repolarization leading to defects in auxin transport, ultimately to leaf venation and vasculature regeneration defects. Our results describe the CAMEL-CANAR receptor complex, which is required for auxin feed-back on its own transport and thus for coordinated tissue polarization during auxin canalization."}],"file_date_updated":"2021-12-08T23:30:03Z","publication_status":"published","date_created":"2020-12-01T12:38:18Z","related_material":{"record":[{"id":"7427","relation":"part_of_dissertation","status":"public"},{"id":"6260","relation":"part_of_dissertation","status":"public"},{"relation":"part_of_dissertation","id":"7500","status":"public"},{"status":"public","relation":"part_of_dissertation","id":"449"},{"status":"public","id":"191","relation":"part_of_dissertation"}]},"file":[{"creator":"jhajny","checksum":"210a9675af5e4c78b0b56d920ac82866","date_created":"2020-12-04T07:27:52Z","access_level":"closed","file_id":"8919","relation":"source_file","file_name":"Jakub Hajný IST Austria final_JH.docx","embargo_to":"open_access","file_size":91279806,"content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","date_updated":"2021-07-16T22:30:03Z"},{"file_name":"Jakub Hajný IST Austria final_JH-merged without Science.pdf","content_type":"application/pdf","date_updated":"2021-12-08T23:30:03Z","file_size":68707697,"embargo":"2021-12-07","relation":"main_file","file_id":"8933","creator":"jhajny","checksum":"1781385b4aa73eba89cc76c6172f71d2","access_level":"open_access","date_created":"2020-12-09T15:04:41Z"}],"ddc":["580"],"month":"12","status":"public","date_updated":"2025-05-07T11:12:31Z","oa_version":"Published Version","citation":{"short":"J. Hajny, Identification and Characterization of the Molecular Machinery of Auxin-Dependent Canalization during Vasculature Formation and Regeneration, Institute of Science and Technology Austria, 2020.","mla":"Hajny, Jakub. <i>Identification and Characterization of the Molecular Machinery of Auxin-Dependent Canalization during Vasculature Formation and Regeneration</i>. Institute of Science and Technology Austria, 2020, doi:<a href=\"https://doi.org/10.15479/AT:ISTA:8822\">10.15479/AT:ISTA:8822</a>.","ista":"Hajny J. 2020. Identification and characterization of the molecular machinery of auxin-dependent canalization during vasculature formation and regeneration. Institute of Science and Technology Austria.","ama":"Hajny J. Identification and characterization of the molecular machinery of auxin-dependent canalization during vasculature formation and regeneration. 2020. doi:<a href=\"https://doi.org/10.15479/AT:ISTA:8822\">10.15479/AT:ISTA:8822</a>","ieee":"J. Hajny, “Identification and characterization of the molecular machinery of auxin-dependent canalization during vasculature formation and regeneration,” Institute of Science and Technology Austria, 2020.","apa":"Hajny, J. (2020). <i>Identification and characterization of the molecular machinery of auxin-dependent canalization during vasculature formation and regeneration</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT:ISTA:8822\">https://doi.org/10.15479/AT:ISTA:8822</a>","chicago":"Hajny, Jakub. “Identification and Characterization of the Molecular Machinery of Auxin-Dependent Canalization during Vasculature Formation and Regeneration.” Institute of Science and Technology Austria, 2020. <a href=\"https://doi.org/10.15479/AT:ISTA:8822\">https://doi.org/10.15479/AT:ISTA:8822</a>."},"has_accepted_license":"1","publisher":"Institute of Science and Technology Austria","article_processing_charge":"No","type":"dissertation"},{"volume":33,"author":[{"last_name":"Tan","orcid":"0000-0002-0471-8285","first_name":"Shutang","id":"2DE75584-F248-11E8-B48F-1D18A9856A87","full_name":"Tan, Shutang"},{"last_name":"Di Donato","full_name":"Di Donato, Martin","first_name":"Martin"},{"last_name":"Glanc","orcid":"0000-0003-0619-7783","id":"1AE1EA24-02D0-11E9-9BAA-DAF4881429F2","first_name":"Matous","full_name":"Glanc, Matous"},{"first_name":"Xixi","orcid":"0000-0001-7048-4627","id":"61A66458-47E9-11EA-85BA-8AEAAF14E49A","full_name":"Zhang, Xixi","last_name":"Zhang"},{"full_name":"Klíma, Petr","first_name":"Petr","last_name":"Klíma"},{"last_name":"Liu","first_name":"Jie","full_name":"Liu, Jie"},{"last_name":"Bailly","full_name":"Bailly, Aurélien","first_name":"Aurélien"},{"last_name":"Ferro","full_name":"Ferro, Noel","first_name":"Noel"},{"first_name":"Jan","full_name":"Petrášek, Jan","last_name":"Petrášek"},{"full_name":"Geisler, Markus","first_name":"Markus","last_name":"Geisler"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří","first_name":"Jiří","last_name":"Friml"}],"intvolume":"        33","date_published":"2020-12-01T00:00:00Z","quality_controlled":"1","issue":"9","_id":"8943","date_created":"2020-12-13T23:01:21Z","external_id":{"pmid":["33264621"],"isi":["000595658100018"]},"publication_status":"published","file_date_updated":"2020-12-14T07:33:39Z","abstract":[{"text":"The widely used non-steroidal anti-inflammatory drugs (NSAIDs) are derivatives of the phytohormone salicylic acid (SA). SA is well known to regulate plant immunity and development, whereas there have been few reports focusing on the effects of NSAIDs in plants. Our studies here reveal that NSAIDs exhibit largely overlapping physiological activities to SA in the model plant Arabidopsis. NSAID treatments lead to shorter and agravitropic primary roots and inhibited lateral root organogenesis. Notably, in addition to the SA-like action, which in roots involves binding to the protein phosphatase 2A (PP2A), NSAIDs also exhibit PP2A-independent effects. Cell biological and biochemical analyses reveal that many NSAIDs bind directly to and inhibit the chaperone activity of TWISTED DWARF1, thereby regulating actin cytoskeleton dynamics and subsequent endosomal trafficking. Our findings uncover an unexpected bioactivity of human pharmaceuticals in plants and provide insights into the molecular mechanism underlying the cellular action of this class of anti-inflammatory compounds.","lang":"eng"}],"scopus_import":"1","file":[{"access_level":"open_access","date_created":"2020-12-14T07:33:39Z","creator":"dernst","checksum":"ed18cba0fb48ed2e789381a54cc21904","file_id":"8948","relation":"main_file","content_type":"application/pdf","date_updated":"2020-12-14T07:33:39Z","file_size":8056434,"success":1,"file_name":"2020_CellReports_Tan.pdf"}],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"related_material":{"link":[{"relation":"press_release","url":"https://ist.ac.at/en/news/plants-on-aspirin/","description":"News on IST Homepage"}]},"oa_version":"Published Version","date_updated":"2023-11-16T13:03:31Z","status":"public","month":"12","isi":1,"ddc":["580"],"article_processing_charge":"Yes","publisher":"Elsevier","has_accepted_license":"1","citation":{"ieee":"S. Tan <i>et al.</i>, “Non-steroidal anti-inflammatory drugs target TWISTED DWARF1-regulated actin dynamics and auxin transport-mediated plant development,” <i>Cell Reports</i>, vol. 33, no. 9. Elsevier, 2020.","apa":"Tan, S., Di Donato, M., Glanc, M., Zhang, X., Klíma, P., Liu, J., … Friml, J. (2020). Non-steroidal anti-inflammatory drugs target TWISTED DWARF1-regulated actin dynamics and auxin transport-mediated plant development. <i>Cell Reports</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.celrep.2020.108463\">https://doi.org/10.1016/j.celrep.2020.108463</a>","chicago":"Tan, Shutang, Martin Di Donato, Matous Glanc, Xixi Zhang, Petr Klíma, Jie Liu, Aurélien Bailly, et al. “Non-Steroidal Anti-Inflammatory Drugs Target TWISTED DWARF1-Regulated Actin Dynamics and Auxin Transport-Mediated Plant Development.” <i>Cell Reports</i>. Elsevier, 2020. <a href=\"https://doi.org/10.1016/j.celrep.2020.108463\">https://doi.org/10.1016/j.celrep.2020.108463</a>.","mla":"Tan, Shutang, et al. “Non-Steroidal Anti-Inflammatory Drugs Target TWISTED DWARF1-Regulated Actin Dynamics and Auxin Transport-Mediated Plant Development.” <i>Cell Reports</i>, vol. 33, no. 9, 108463, Elsevier, 2020, doi:<a href=\"https://doi.org/10.1016/j.celrep.2020.108463\">10.1016/j.celrep.2020.108463</a>.","short":"S. Tan, M. Di Donato, M. Glanc, X. Zhang, P. Klíma, J. Liu, A. Bailly, N. Ferro, J. Petrášek, M. Geisler, J. Friml, Cell Reports 33 (2020).","ista":"Tan S, Di Donato M, Glanc M, Zhang X, Klíma P, Liu J, Bailly A, Ferro N, Petrášek J, Geisler M, Friml J. 2020. Non-steroidal anti-inflammatory drugs target TWISTED DWARF1-regulated actin dynamics and auxin transport-mediated plant development. Cell Reports. 33(9), 108463.","ama":"Tan S, Di Donato M, Glanc M, et al. Non-steroidal anti-inflammatory drugs target TWISTED DWARF1-regulated actin dynamics and auxin transport-mediated plant development. <i>Cell Reports</i>. 2020;33(9). doi:<a href=\"https://doi.org/10.1016/j.celrep.2020.108463\">10.1016/j.celrep.2020.108463</a>"},"type":"journal_article","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"}],"pmid":1,"acknowledgement":"We thank Drs. Sebastian Bednarek (University of Wisconsin-Madison), Niko Geldner (University of Lausanne), and Karin Schumacher (Heidelberg University) for kindly sharing published Arabidopsis lines; Dr. Satoshi Naramoto for the pPIN2::PIN2-GFP; pVHA-a1::VHA-a1-mRFP reporter; the staff at the Life Science Facility and Bioimaging Facility, Monika Hrtyan, and Dorota Jaworska at IST Austria for technical support; and Drs. Su Tang (Texas A&M University),\r\nMelinda Abas (BOKU), Eva Benkova´ (IST Austria), Christian Luschnig (BOKU), Bartel Vanholme (Gent University), and the Friml group for valuable discussions. The research leading to these findings was funded by the European Union’s Horizon 2020 program (ERC grant agreement no. 742985, to J.F.), the People Programme (Marie Curie Actions) of the European Union’s Seventh Framework Programme (FP7/2007-2013) under REA grant agreement no.\r\n291734, the Swiss National Funds (31003A_165877, to M.G.), the Ministry of Education, Youth, and Sports of the Czech Republic (project no. CZ.02.1.01/0.0/0.0/16_019/0000738, EU Operational Programme ‘‘Research, development and education and Centre for Plant Experimental Biology’’), and the EU Operational Programme Prague - Competitiveness (project no. CZ.2.16/3.1.00/21519). S.T. was funded by a European Molecular Biology Organization (EMBO) long-term postdoctoral fellowship (ALTF 723-2015). X.Z. was partly supported by a PhD scholarship from the China Scholarship Council.","project":[{"call_identifier":"H2020","grant_number":"742985","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","_id":"261099A6-B435-11E9-9278-68D0E5697425"},{"call_identifier":"FP7","grant_number":"291734","name":"International IST Postdoc Fellowship Programme","_id":"25681D80-B435-11E9-9278-68D0E5697425"},{"grant_number":"723-2015","name":"Long Term Fellowship","_id":"256FEF10-B435-11E9-9278-68D0E5697425"}],"department":[{"_id":"JiFr"}],"year":"2020","title":"Non-steroidal anti-inflammatory drugs target TWISTED DWARF1-regulated actin dynamics and auxin transport-mediated plant development","day":"01","publication":"Cell Reports","doi":"10.1016/j.celrep.2020.108463","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","oa":1,"publication_identifier":{"eissn":["22111247"]},"language":[{"iso":"eng"}],"article_number":"108463","article_type":"original","ec_funded":1},{"title":"Functional innovations of PIN auxin transporters mark crucial evolutionary transitions during rise of flowering plants","pmid":1,"project":[{"call_identifier":"H2020","grant_number":"742985","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","_id":"261099A6-B435-11E9-9278-68D0E5697425"},{"call_identifier":"FWF","name":"Molecular mechanisms of endocytic cargo recognition in plants","_id":"26538374-B435-11E9-9278-68D0E5697425","grant_number":"I03630"},{"name":"A Case Study of Plant Growth Regulation: Molecular Mechanism of Auxin-mediated Rapid Growth Inhibition in Arabidopsis Root","_id":"26B4D67E-B435-11E9-9278-68D0E5697425","grant_number":"25351"}],"acknowledgement":"We thank C.Löhne (Botanic Gardens, University of Bonn) for providing us with A. trichopoda. We would like to thank T.Han, A.Mally (IST, Austria), and C.Hartinger (University of Oxford) for constructive comment and careful reading. Funding: The research leading to these results has received funding from the European Union’s Horizon 2020 Research and Innovation Programme (ERC grant agreement number 742985), Austrian Science Fund (FWF, grant number I 3630-B25), DOC Fellowship of the Austrian Academy of Sciences, and IST Fellow program. ","year":"2020","department":[{"_id":"JiFr"}],"oa":1,"publication_identifier":{"eissn":["2375-2548"]},"language":[{"iso":"eng"}],"article_number":"eabc8895","article_type":"original","ec_funded":1,"publication":"Science Advances","day":"11","doi":"10.1126/sciadv.abc8895","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","external_id":{"pmid":["33310852"],"isi":["000599903600014"]},"publication_status":"published","date_created":"2021-01-03T23:01:23Z","file_date_updated":"2021-01-07T12:44:33Z","abstract":[{"text":"Flowering plants display the highest diversity among plant species and have notably shaped terrestrial landscapes. Nonetheless, the evolutionary origin of their unprecedented morphological complexity remains largely an enigma. Here, we show that the coevolution of cis-regulatory and coding regions of PIN-FORMED (PIN) auxin transporters confined their expression to certain cell types and directed their subcellular localization to particular cell sides, which together enabled dynamic auxin gradients across tissues critical to the complex architecture of flowering plants. Extensive intraspecies and interspecies genetic complementation experiments with PINs from green alga up to flowering plant lineages showed that PIN genes underwent three subsequent, critical evolutionary innovations and thus acquired a triple function to regulate the development of three essential components of the flowering plant Arabidopsis: shoot/root, inflorescence, and floral organ. Our work highlights the critical role of functional innovations within the PIN gene family as essential prerequisites for the origin of flowering plants.","lang":"eng"}],"scopus_import":"1","date_published":"2020-12-11T00:00:00Z","intvolume":"         6","volume":6,"author":[{"orcid":"0000-0003-2627-6956","full_name":"Zhang, Yuzhou","id":"3B6137F2-F248-11E8-B48F-1D18A9856A87","first_name":"Yuzhou","last_name":"Zhang"},{"id":"3922B506-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-7244-7237","first_name":"Lesia","full_name":"Rodriguez Solovey, Lesia","last_name":"Rodriguez Solovey"},{"last_name":"Li","orcid":"0000-0002-5607-272X","id":"367EF8FA-F248-11E8-B48F-1D18A9856A87","full_name":"Li, Lanxin","first_name":"Lanxin"},{"last_name":"Zhang","orcid":"0000-0001-7048-4627","id":"61A66458-47E9-11EA-85BA-8AEAAF14E49A","first_name":"Xixi","full_name":"Zhang, Xixi"},{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","first_name":"Jiří","last_name":"Friml"}],"quality_controlled":"1","license":"https://creativecommons.org/licenses/by-nc/4.0/","issue":"50","_id":"8986","article_processing_charge":"No","citation":{"ama":"Zhang Y, Rodriguez Solovey L, Li L, Zhang X, Friml J. Functional innovations of PIN auxin transporters mark crucial evolutionary transitions during rise of flowering plants. <i>Science Advances</i>. 2020;6(50). doi:<a href=\"https://doi.org/10.1126/sciadv.abc8895\">10.1126/sciadv.abc8895</a>","ista":"Zhang Y, Rodriguez Solovey L, Li L, Zhang X, Friml J. 2020. Functional innovations of PIN auxin transporters mark crucial evolutionary transitions during rise of flowering plants. Science Advances. 6(50), eabc8895.","short":"Y. Zhang, L. Rodriguez Solovey, L. Li, X. Zhang, J. Friml, Science Advances 6 (2020).","mla":"Zhang, Yuzhou, et al. “Functional Innovations of PIN Auxin Transporters Mark Crucial Evolutionary Transitions during Rise of Flowering Plants.” <i>Science Advances</i>, vol. 6, no. 50, eabc8895, AAAS, 2020, doi:<a href=\"https://doi.org/10.1126/sciadv.abc8895\">10.1126/sciadv.abc8895</a>.","chicago":"Zhang, Yuzhou, Lesia Rodriguez Solovey, Lanxin Li, Xixi Zhang, and Jiří Friml. “Functional Innovations of PIN Auxin Transporters Mark Crucial Evolutionary Transitions during Rise of Flowering Plants.” <i>Science Advances</i>. AAAS, 2020. <a href=\"https://doi.org/10.1126/sciadv.abc8895\">https://doi.org/10.1126/sciadv.abc8895</a>.","apa":"Zhang, Y., Rodriguez Solovey, L., Li, L., Zhang, X., &#38; Friml, J. (2020). Functional innovations of PIN auxin transporters mark crucial evolutionary transitions during rise of flowering plants. <i>Science Advances</i>. AAAS. <a href=\"https://doi.org/10.1126/sciadv.abc8895\">https://doi.org/10.1126/sciadv.abc8895</a>","ieee":"Y. Zhang, L. Rodriguez Solovey, L. Li, X. Zhang, and J. Friml, “Functional innovations of PIN auxin transporters mark crucial evolutionary transitions during rise of flowering plants,” <i>Science Advances</i>, vol. 6, no. 50. AAAS, 2020."},"has_accepted_license":"1","publisher":"AAAS","type":"journal_article","related_material":{"record":[{"id":"10083","relation":"dissertation_contains","status":"public"}]},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","short":"CC BY-NC (4.0)","name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","image":"/images/cc_by_nc.png"},"file":[{"creator":"dernst","checksum":"5ac2500b191c08ef6dab5327f40ff663","access_level":"open_access","date_created":"2021-01-07T12:44:33Z","file_id":"8994","relation":"main_file","file_name":"2020_ScienceAdvances_Zhang.pdf","date_updated":"2021-01-07T12:44:33Z","content_type":"application/pdf","success":1,"file_size":10578145}],"date_updated":"2024-10-29T10:22:43Z","oa_version":"Published Version","isi":1,"ddc":["580"],"month":"12","status":"public"},{"article_type":"original","ec_funded":1,"oa":1,"publication_identifier":{"eissn":["1469-8137"],"issn":["0028-646x"]},"language":[{"iso":"eng"}],"doi":"10.1111/nph.16203","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","publication":"New Phytologist","day":"01","title":"Auxin guides roots to avoid obstacles during gravitropic growth","project":[{"grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","call_identifier":"H2020"},{"grant_number":"I03630","_id":"26538374-B435-11E9-9278-68D0E5697425","name":"Molecular mechanisms of endocytic cargo recognition in plants","call_identifier":"FWF"},{"call_identifier":"FP7","grant_number":"291734","_id":"25681D80-B435-11E9-9278-68D0E5697425","name":"International IST Postdoc Fellowship Programme"}],"year":"2020","department":[{"_id":"JiFr"}],"pmid":1,"type":"journal_article","article_processing_charge":"Yes (via OA deal)","citation":{"mla":"Zhang, Yuzhou, and Jiří Friml. “Auxin Guides Roots to Avoid Obstacles during Gravitropic Growth.” <i>New Phytologist</i>, vol. 225, no. 3, Wiley, 2020, pp. 1049–52, doi:<a href=\"https://doi.org/10.1111/nph.16203\">10.1111/nph.16203</a>.","short":"Y. Zhang, J. Friml, New Phytologist 225 (2020) 1049–1052.","ista":"Zhang Y, Friml J. 2020. Auxin guides roots to avoid obstacles during gravitropic growth. New Phytologist. 225(3), 1049–1052.","ama":"Zhang Y, Friml J. Auxin guides roots to avoid obstacles during gravitropic growth. <i>New Phytologist</i>. 2020;225(3):1049-1052. doi:<a href=\"https://doi.org/10.1111/nph.16203\">10.1111/nph.16203</a>","apa":"Zhang, Y., &#38; Friml, J. (2020). Auxin guides roots to avoid obstacles during gravitropic growth. <i>New Phytologist</i>. Wiley. <a href=\"https://doi.org/10.1111/nph.16203\">https://doi.org/10.1111/nph.16203</a>","ieee":"Y. Zhang and J. Friml, “Auxin guides roots to avoid obstacles during gravitropic growth,” <i>New Phytologist</i>, vol. 225, no. 3. Wiley, pp. 1049–1052, 2020.","chicago":"Zhang, Yuzhou, and Jiří Friml. “Auxin Guides Roots to Avoid Obstacles during Gravitropic Growth.” <i>New Phytologist</i>. Wiley, 2020. <a href=\"https://doi.org/10.1111/nph.16203\">https://doi.org/10.1111/nph.16203</a>."},"has_accepted_license":"1","publisher":"Wiley","date_updated":"2023-08-17T14:01:49Z","oa_version":"Published Version","isi":1,"ddc":["580"],"status":"public","month":"02","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"file":[{"success":1,"file_size":717345,"content_type":"application/pdf","date_updated":"2020-11-18T16:42:48Z","file_name":"2020_NewPhytologist_Zhang.pdf","relation":"main_file","file_id":"8772","date_created":"2020-11-18T16:42:48Z","access_level":"open_access","checksum":"cd42ffdb381fd52812b9583d4d407139","creator":"dernst"}],"scopus_import":"1","external_id":{"pmid":["31603260"],"isi":["000489638800001"]},"date_created":"2019-11-12T11:41:32Z","publication_status":"published","file_date_updated":"2020-11-18T16:42:48Z","quality_controlled":"1","issue":"3","_id":"6997","intvolume":"       225","date_published":"2020-02-01T00:00:00Z","volume":225,"page":"1049-1052","author":[{"last_name":"Zhang","orcid":"0000-0003-2627-6956","id":"3B6137F2-F248-11E8-B48F-1D18A9856A87","full_name":"Zhang, Yuzhou","first_name":"Yuzhou"},{"full_name":"Friml, Jiří","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","last_name":"Friml"}]},{"type":"journal_article","publisher":"Elsevier","citation":{"short":"M.C. Gallei, C. Luschnig, J. Friml, Current Opinion in Plant Biology 53 (2020) 43–49.","mla":"Gallei, Michelle C., et al. “Auxin Signalling in Growth: Schrödinger’s Cat out of the Bag.” <i>Current Opinion in Plant Biology</i>, vol. 53, no. 2, Elsevier, 2020, pp. 43–49, doi:<a href=\"https://doi.org/10.1016/j.pbi.2019.10.003\">10.1016/j.pbi.2019.10.003</a>.","ama":"Gallei MC, Luschnig C, Friml J. Auxin signalling in growth: Schrödinger’s cat out of the bag. <i>Current Opinion in Plant Biology</i>. 2020;53(2):43-49. doi:<a href=\"https://doi.org/10.1016/j.pbi.2019.10.003\">10.1016/j.pbi.2019.10.003</a>","ista":"Gallei MC, Luschnig C, Friml J. 2020. Auxin signalling in growth: Schrödinger’s cat out of the bag. Current Opinion in Plant Biology. 53(2), 43–49.","apa":"Gallei, M. C., Luschnig, C., &#38; Friml, J. (2020). Auxin signalling in growth: Schrödinger’s cat out of the bag. <i>Current Opinion in Plant Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.pbi.2019.10.003\">https://doi.org/10.1016/j.pbi.2019.10.003</a>","ieee":"M. C. Gallei, C. Luschnig, and J. Friml, “Auxin signalling in growth: Schrödinger’s cat out of the bag,” <i>Current Opinion in Plant Biology</i>, vol. 53, no. 2. Elsevier, pp. 43–49, 2020.","chicago":"Gallei, Michelle C, Christian Luschnig, and Jiří Friml. “Auxin Signalling in Growth: Schrödinger’s Cat out of the Bag.” <i>Current Opinion in Plant Biology</i>. Elsevier, 2020. <a href=\"https://doi.org/10.1016/j.pbi.2019.10.003\">https://doi.org/10.1016/j.pbi.2019.10.003</a>."},"article_processing_charge":"No","month":"02","status":"public","isi":1,"oa_version":"None","date_updated":"2023-08-17T14:07:22Z","related_material":{"record":[{"relation":"dissertation_contains","id":"11626","status":"public"}]},"scopus_import":"1","abstract":[{"text":"The phytohormone auxin acts as an amazingly versatile coordinator of plant growth and development. With its morphogen-like properties, auxin controls sites and timing of differentiation and/or growth responses both, in quantitative and qualitative terms. Specificity in the auxin response depends largely on distinct modes of signal transmission, by which individual cells perceive and convert auxin signals into a remarkable diversity of responses. The best understood, or so-called canonical mechanism of auxin perception ultimately results in variable adjustments of the cellular transcriptome, via a short, nuclear signal transduction pathway. Additional findings that accumulated over decades implied that an additional, presumably, cell surface-based auxin perception mechanism mediates very rapid cellular responses and decisively contributes to the cell's overall hormonal response. Recent investigations into both, nuclear and cell surface auxin signalling challenged this assumed partition of roles for different auxin signalling pathways and revealed an unexpected complexity in transcriptional and non-transcriptional cellular responses mediated by auxin.","lang":"eng"}],"external_id":{"isi":["000521120600007"],"pmid":["31760231"]},"publication_status":"published","date_created":"2019-12-02T12:05:26Z","_id":"7142","issue":"2","quality_controlled":"1","author":[{"last_name":"Gallei","orcid":"0000-0003-1286-7368","id":"35A03822-F248-11E8-B48F-1D18A9856A87","full_name":"Gallei, Michelle C","first_name":"Michelle C"},{"full_name":"Luschnig, Christian","first_name":"Christian","last_name":"Luschnig"},{"full_name":"Friml, Jiří","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","last_name":"Friml"}],"page":"43-49","volume":53,"date_published":"2020-02-01T00:00:00Z","intvolume":"        53","ec_funded":1,"article_type":"original","language":[{"iso":"eng"}],"publication_identifier":{"issn":["1369-5266"],"eissn":["1879-0356"]},"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","doi":"10.1016/j.pbi.2019.10.003","day":"01","publication":"Current Opinion in Plant Biology","title":"Auxin signalling in growth: Schrödinger's cat out of the bag","department":[{"_id":"JiFr"}],"year":"2020","acknowledgement":"Research in J.F. laboratory is funded by the European Union's Horizon 2020 program (ERC grant agreement n° 742985); C.L. is supported by the Austrian Science Fund (FWF grant P 31493).","project":[{"call_identifier":"H2020","grant_number":"742985","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","_id":"261099A6-B435-11E9-9278-68D0E5697425"}],"pmid":1}]