[{"file":[{"file_name":"IST-2016-441-v1+1_140017.full.pdf","content_type":"application/pdf","date_updated":"2020-07-14T12:45:31Z","file_size":682570,"checksum":"2020627feff36cf0799167c84149fa75","date_created":"2018-12-12T10:13:40Z","creator":"system","file_id":"5025","access_level":"open_access","relation":"main_file"}],"status":"public","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","publist_id":"4786","oa":1,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"type":"journal_article","date_published":"2014-04-16T00:00:00Z","language":[{"iso":"eng"}],"oa_version":"Published Version","article_number":"140017","month":"04","has_accepted_license":"1","publication":"Open Biology","volume":4,"acknowledgement":"This work was supported by a grant from the Research Foundation-Flanders (Odysseus).\r\n\r\n","ddc":["570"],"day":"16","doi":"10.1098/rsob.140017","abstract":[{"lang":"eng","text":"Although plant and animal cells use a similar core mechanism to deliver proteins to the plasma membrane, their different lifestyle, body organization and specific cell structures resulted in the acquisition of regulatory mechanisms that vary in the two kingdoms. In particular, cell polarity regulators do not seem to be conserved, because genes encoding key components are absent in plant genomes. In plants, the broad knowledge on polarity derives from the study of auxin transporters, the PIN-FORMED proteins, in the model plant Arabidopsis thaliana. In animals, much information is provided from the study of polarity in epithelial cells that exhibit basolateral and luminal apical polarities, separated by tight junctions. In this review, we summarize the similarities and differences of the polarization mechanisms between plants and animals and survey the main genetic approaches that have been used to characterize new genes involved in polarity establishment in plants, including the frequently used forward and reverse genetics screens as well as a novel chemical genetics approach that is expected to overcome the limitation of classical genetics methods."}],"year":"2014","citation":{"ieee":"U. Kania, M. Fendrych, and J. Friml, “Polar delivery in plants; commonalities and differences to animal epithelial cells,” <i>Open Biology</i>, vol. 4, no. APRIL. Royal Society, 2014.","chicago":"Kania, Urszula, Matyas Fendrych, and Jiří Friml. “Polar Delivery in Plants; Commonalities and Differences to Animal Epithelial Cells.” <i>Open Biology</i>. Royal Society, 2014. <a href=\"https://doi.org/10.1098/rsob.140017\">https://doi.org/10.1098/rsob.140017</a>.","apa":"Kania, U., Fendrych, M., &#38; Friml, J. (2014). Polar delivery in plants; commonalities and differences to animal epithelial cells. <i>Open Biology</i>. Royal Society. <a href=\"https://doi.org/10.1098/rsob.140017\">https://doi.org/10.1098/rsob.140017</a>","ama":"Kania U, Fendrych M, Friml J. Polar delivery in plants; commonalities and differences to animal epithelial cells. <i>Open Biology</i>. 2014;4(APRIL). doi:<a href=\"https://doi.org/10.1098/rsob.140017\">10.1098/rsob.140017</a>","ista":"Kania U, Fendrych M, Friml J. 2014. Polar delivery in plants; commonalities and differences to animal epithelial cells. Open Biology. 4(APRIL), 140017.","mla":"Kania, Urszula, et al. “Polar Delivery in Plants; Commonalities and Differences to Animal Epithelial Cells.” <i>Open Biology</i>, vol. 4, no. APRIL, 140017, Royal Society, 2014, doi:<a href=\"https://doi.org/10.1098/rsob.140017\">10.1098/rsob.140017</a>.","short":"U. Kania, M. Fendrych, J. Friml, Open Biology 4 (2014)."},"date_updated":"2021-01-12T06:55:52Z","publisher":"Royal Society","quality_controlled":"1","file_date_updated":"2020-07-14T12:45:31Z","date_created":"2018-12-11T11:56:13Z","department":[{"_id":"JiFr"}],"publication_status":"published","intvolume":"         4","title":"Polar delivery in plants; commonalities and differences to animal epithelial cells","pubrep_id":"441","scopus_import":1,"_id":"2188","issue":"APRIL","author":[{"id":"4AE5C486-F248-11E8-B48F-1D18A9856A87","first_name":"Urszula","last_name":"Kania","full_name":"Kania, Urszula"},{"full_name":"Fendrych, Matyas","first_name":"Matyas","last_name":"Fendrych"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiřĺ","orcid":"0000-0002-8302-7596","last_name":"Friml","first_name":"Jiřĺ"}]},{"user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","status":"public","publist_id":"4742","publication_identifier":{"issn":["00320781"]},"date_published":"2014-04-01T00:00:00Z","type":"journal_article","language":[{"iso":"eng"}],"month":"04","oa_version":"None","project":[{"grant_number":"282300","name":"Polarity and subcellular dynamics in plants","call_identifier":"FP7","_id":"25716A02-B435-11E9-9278-68D0E5697425"}],"publication":"Plant and Cell Physiology","volume":55,"abstract":[{"lang":"eng","text":"Leaf venation develops complex patterns in angiosperms, but the mechanism underlying this process is largely unknown. To elucidate the molecular mechanisms governing vein pattern formation, we previously isolated vascular network defective (van) mutants that displayed venation discontinuities. Here, we report the phenotypic analysis of van4 mutants, and we identify and characterize the VAN4 gene. Detailed phenotypic analysis shows that van4 mutants are defective in procambium cell differentiation and subsequent vascular cell differentiation. Reduced shoot and root cell growth is observed in van4 mutants, suggesting that VAN4 function is important for cell growth and the establishment of venation continuity. Consistent with these phenotypes, the VAN4 gene is strongly expressed in vascular and meristematic cells. VAN4 encodes a putative TRS120, which is a known guanine nucleotide exchange factor (GEF) for Rab GTPase involved in regulating vesicle transport, and a known tethering factor that determines the specificity of membrane fusion. VAN4 protein localizes at the trans-Golgi network/early endosome (TGN/EE). Aberrant recycling of the auxin efflux carrier PIN proteins is observed in van4 mutants. These results suggest that VAN4-mediated exocytosis at the TGN plays important roles in plant vascular development and cell growth in shoot and root. Our identification of VAN4 as a putative TRS120 shows that Rab GTPases are crucial (in addition to ARF GTPases) for continuous vascular development, and provides further evidence for the importance of vesicle transport in leaf vascular formation."}],"doi":"10.1093/pcp/pcu012","day":"01","date_updated":"2021-01-12T06:56:06Z","citation":{"chicago":"Naramoto, Satoshi, Tomasz Nodzyński, Tomoko Dainobu, Hirotomo Takatsuka, Teruyo Okada, Jiří Friml, and Hiroo Fukuda. “VAN4 Encodes a Putative TRS120 That Is Required for Normal Cell Growth and Vein Development in Arabidopsis.” <i>Plant and Cell Physiology</i>. Oxford University Press, 2014. <a href=\"https://doi.org/10.1093/pcp/pcu012\">https://doi.org/10.1093/pcp/pcu012</a>.","ieee":"S. Naramoto <i>et al.</i>, “VAN4 encodes a putative TRS120 that is required for normal cell growth and vein development in arabidopsis,” <i>Plant and Cell Physiology</i>, vol. 55, no. 4. Oxford University Press, pp. 750–763, 2014.","apa":"Naramoto, S., Nodzyński, T., Dainobu, T., Takatsuka, H., Okada, T., Friml, J., &#38; Fukuda, H. (2014). VAN4 encodes a putative TRS120 that is required for normal cell growth and vein development in arabidopsis. <i>Plant and Cell Physiology</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/pcp/pcu012\">https://doi.org/10.1093/pcp/pcu012</a>","ama":"Naramoto S, Nodzyński T, Dainobu T, et al. VAN4 encodes a putative TRS120 that is required for normal cell growth and vein development in arabidopsis. <i>Plant and Cell Physiology</i>. 2014;55(4):750-763. doi:<a href=\"https://doi.org/10.1093/pcp/pcu012\">10.1093/pcp/pcu012</a>","ista":"Naramoto S, Nodzyński T, Dainobu T, Takatsuka H, Okada T, Friml J, Fukuda H. 2014. VAN4 encodes a putative TRS120 that is required for normal cell growth and vein development in arabidopsis. Plant and Cell Physiology. 55(4), 750–763.","short":"S. Naramoto, T. Nodzyński, T. Dainobu, H. Takatsuka, T. Okada, J. Friml, H. Fukuda, Plant and Cell Physiology 55 (2014) 750–763.","mla":"Naramoto, Satoshi, et al. “VAN4 Encodes a Putative TRS120 That Is Required for Normal Cell Growth and Vein Development in Arabidopsis.” <i>Plant and Cell Physiology</i>, vol. 55, no. 4, Oxford University Press, 2014, pp. 750–63, doi:<a href=\"https://doi.org/10.1093/pcp/pcu012\">10.1093/pcp/pcu012</a>."},"year":"2014","publisher":"Oxford University Press","page":"750 - 763","ec_funded":1,"quality_controlled":"1","title":"VAN4 encodes a putative TRS120 that is required for normal cell growth and vein development in arabidopsis","intvolume":"        55","publication_status":"published","department":[{"_id":"JiFr"}],"date_created":"2018-12-11T11:56:24Z","author":[{"full_name":"Naramoto, Satoshi","first_name":"Satoshi","last_name":"Naramoto"},{"first_name":"Tomasz","last_name":"Nodzyński","full_name":"Nodzyński, Tomasz"},{"full_name":"Dainobu, Tomoko","last_name":"Dainobu","first_name":"Tomoko"},{"full_name":"Takatsuka, Hirotomo","first_name":"Hirotomo","last_name":"Takatsuka"},{"last_name":"Okada","first_name":"Teruyo","full_name":"Okada, Teruyo"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596","last_name":"Friml","first_name":"Jirí"},{"last_name":"Fukuda","first_name":"Hiroo","full_name":"Fukuda, Hiroo"}],"issue":"4","_id":"2222","scopus_import":1},{"main_file_link":[{"open_access":"1","url":"http://repository.ist.ac.at/id/eprint/431"}],"file":[{"creator":"system","file_id":"5076","relation":"main_file","access_level":"open_access","content_type":"application/pdf","file_name":"IST-2016-431-v1+1_Plant_Cell_Physiol-2014-Tanaka-737-49.pdf","date_updated":"2020-07-14T12:45:34Z","checksum":"b781a76b32ac35a520256453c3ba9433","file_size":2028111,"date_created":"2018-12-12T10:14:25Z"}],"user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","status":"public","tmp":{"name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","image":"/images/cc_by_nc.png","short":"CC BY-NC (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode"},"date_published":"2014-04-01T00:00:00Z","type":"journal_article","publication_identifier":{"issn":["00320781"]},"publist_id":"4741","oa":1,"language":[{"iso":"eng"}],"publication":"Plant and Cell Physiology","has_accepted_license":"1","oa_version":"Published Version","project":[{"call_identifier":"FP7","_id":"25716A02-B435-11E9-9278-68D0E5697425","grant_number":"282300","name":"Polarity and subcellular dynamics in plants"},{"name":"Innovationsförderung in der Grenzregion Österreich – Tschechische Republik durch die Schaffung von Synergien im Bereich der Forschungsinfrastruktur","_id":"256BDAB0-B435-11E9-9278-68D0E5697425"}],"month":"04","volume":55,"ddc":["570"],"date_updated":"2021-01-12T06:56:07Z","citation":{"apa":"Tanaka, H., Nodzyński, T., Kitakura, S., Feraru, M., Sasabe, M., Ishikawa, T., … Friml, J. (2014). BEX1/ARF1A1C is required for BFA-sensitive recycling of PIN auxin transporters and auxin-mediated development in arabidopsis. <i>Plant and Cell Physiology</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/pcp/pct196\">https://doi.org/10.1093/pcp/pct196</a>","ama":"Tanaka H, Nodzyński T, Kitakura S, et al. BEX1/ARF1A1C is required for BFA-sensitive recycling of PIN auxin transporters and auxin-mediated development in arabidopsis. <i>Plant and Cell Physiology</i>. 2014;55(4):737-749. doi:<a href=\"https://doi.org/10.1093/pcp/pct196\">10.1093/pcp/pct196</a>","ieee":"H. Tanaka <i>et al.</i>, “BEX1/ARF1A1C is required for BFA-sensitive recycling of PIN auxin transporters and auxin-mediated development in arabidopsis,” <i>Plant and Cell Physiology</i>, vol. 55, no. 4. Oxford University Press, pp. 737–749, 2014.","chicago":"Tanaka, Hirokazu, Tomasz Nodzyński, Saeko Kitakura, Mugurel Feraru, Michiko Sasabe, Tomomi Ishikawa, Jürgen Kleine Vehn, Tatsuo Kakimoto, and Jiří Friml. “BEX1/ARF1A1C Is Required for BFA-Sensitive Recycling of PIN Auxin Transporters and Auxin-Mediated Development in Arabidopsis.” <i>Plant and Cell Physiology</i>. Oxford University Press, 2014. <a href=\"https://doi.org/10.1093/pcp/pct196\">https://doi.org/10.1093/pcp/pct196</a>.","short":"H. Tanaka, T. Nodzyński, S. Kitakura, M. Feraru, M. Sasabe, T. Ishikawa, J. Kleine Vehn, T. Kakimoto, J. Friml, Plant and Cell Physiology 55 (2014) 737–749.","mla":"Tanaka, Hirokazu, et al. “BEX1/ARF1A1C Is Required for BFA-Sensitive Recycling of PIN Auxin Transporters and Auxin-Mediated Development in Arabidopsis.” <i>Plant and Cell Physiology</i>, vol. 55, no. 4, Oxford University Press, 2014, pp. 737–49, doi:<a href=\"https://doi.org/10.1093/pcp/pct196\">10.1093/pcp/pct196</a>.","ista":"Tanaka H, Nodzyński T, Kitakura S, Feraru M, Sasabe M, Ishikawa T, Kleine Vehn J, Kakimoto T, Friml J. 2014. BEX1/ARF1A1C is required for BFA-sensitive recycling of PIN auxin transporters and auxin-mediated development in arabidopsis. Plant and Cell Physiology. 55(4), 737–749."},"year":"2014","doi":"10.1093/pcp/pct196","day":"01","abstract":[{"lang":"eng","text":"Correct positioning of membrane proteins is an essential process in eukaryotic organisms. The plant hormone auxin is distributed through intercellular transport and triggers various cellular responses. Auxin transporters of the PIN-FORMED (PIN) family localize asymmetrically at the plasma membrane (PM) and mediate the directional transport of auxin between cells. A fungal toxin, brefeldin A (BFA), inhibits a subset of guanine nucleotide exchange factors for ADP-ribosylation factor small GTPases (ARF GEFs) including GNOM, which plays a major role in localization of PIN1 predominantly to the basal side of the PM. The Arabidopsis genome encodes 19 ARF-related putative GTPases. However, ARF components involved in PIN1 localization have been genetically poorly defined. Using a fluorescence imaging-based forward genetic approach, we identified an Arabidopsis mutant, bfa-visualized exocytic trafficking defective1 (bex1), in which PM localization of PIN1-green fluorescent protein (GFP) as well as development is hypersensitive to BFA. We found that in bex1 a member of the ARF1 gene family, ARF1A1C, was mutated. ARF1A1C localizes to the trans-Golgi network/early endosome and Golgi apparatus, acts synergistically to BEN1/MIN7 ARF GEF and is important for PIN recycling to the PM. Consistent with the developmental importance of PIN proteins, functional interference with ARF1 resulted in an impaired auxin response gradient and various developmental defects including embryonic patterning defects and growth arrest. Our results show that ARF1A1C is essential for recycling of PIN auxin transporters and for various auxin-dependent developmental processes."}],"page":"737 - 749","ec_funded":1,"quality_controlled":"1","file_date_updated":"2020-07-14T12:45:34Z","publisher":"Oxford University Press","_id":"2223","license":"https://creativecommons.org/licenses/by-nc/4.0/","scopus_import":1,"author":[{"last_name":"Tanaka","first_name":"Hirokazu","full_name":"Tanaka, Hirokazu"},{"full_name":"Nodzyński, Tomasz","last_name":"Nodzyński","first_name":"Tomasz"},{"full_name":"Kitakura, Saeko","first_name":"Saeko","last_name":"Kitakura"},{"last_name":"Feraru","first_name":"Mugurel","full_name":"Feraru, Mugurel"},{"last_name":"Sasabe","first_name":"Michiko","full_name":"Sasabe, Michiko"},{"full_name":"Ishikawa, Tomomi","first_name":"Tomomi","last_name":"Ishikawa"},{"first_name":"Jürgen","last_name":"Kleine Vehn","full_name":"Kleine Vehn, Jürgen"},{"first_name":"Tatsuo","last_name":"Kakimoto","full_name":"Kakimoto, Tatsuo"},{"last_name":"Friml","first_name":"Jirí","full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"issue":"4","publication_status":"published","date_created":"2018-12-11T11:56:25Z","department":[{"_id":"JiFr"}],"title":"BEX1/ARF1A1C is required for BFA-sensitive recycling of PIN auxin transporters and auxin-mediated development in arabidopsis","pubrep_id":"431","intvolume":"        55"},{"language":[{"iso":"eng"}],"page":"1 - 10","quality_controlled":"1","publisher":"Springer","author":[{"first_name":"Eduardo","last_name":"Cires Rodriguez","full_name":"Cires Rodriguez, Eduardo","id":"2AD56A7A-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Baltisberger, Matthias","first_name":"Matthias","last_name":"Baltisberger"},{"id":"33A3C818-F248-11E8-B48F-1D18A9856A87","last_name":"Cuesta","first_name":"Candela","full_name":"Cuesta, Candela","orcid":"0000-0003-1923-2410"},{"first_name":"Pablo","last_name":"Vargas","full_name":"Vargas, Pablo"},{"full_name":"Prieto, José","last_name":"Prieto","first_name":"José"}],"issue":"1","publication":"Organisms Diversity and Evolution","_id":"2227","scopus_import":"1","month":"03","title":"Allopolyploid origin of the Balkan endemic Ranunculus wettsteinii (Ranunculaceae) inferred from nuclear and plastid DNA sequences","intvolume":"        14","oa_version":"None","publication_status":"published","article_processing_charge":"No","date_created":"2018-12-11T11:56:26Z","department":[{"_id":"JiFr"},{"_id":"EvBe"}],"user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","status":"public","volume":14,"date_published":"2014-03-01T00:00:00Z","type":"journal_article","date_updated":"2022-08-25T14:42:46Z","citation":{"ieee":"E. Cires Rodriguez, M. Baltisberger, C. Cuesta, P. Vargas, and J. Prieto, “Allopolyploid origin of the Balkan endemic Ranunculus wettsteinii (Ranunculaceae) inferred from nuclear and plastid DNA sequences,” <i>Organisms Diversity and Evolution</i>, vol. 14, no. 1. Springer, pp. 1–10, 2014.","chicago":"Cires Rodriguez, Eduardo, Matthias Baltisberger, Candela Cuesta, Pablo Vargas, and José Prieto. “Allopolyploid Origin of the Balkan Endemic Ranunculus Wettsteinii (Ranunculaceae) Inferred from Nuclear and Plastid DNA Sequences.” <i>Organisms Diversity and Evolution</i>. Springer, 2014. <a href=\"https://doi.org/10.1007/s13127-013-0150-6\">https://doi.org/10.1007/s13127-013-0150-6</a>.","apa":"Cires Rodriguez, E., Baltisberger, M., Cuesta, C., Vargas, P., &#38; Prieto, J. (2014). Allopolyploid origin of the Balkan endemic Ranunculus wettsteinii (Ranunculaceae) inferred from nuclear and plastid DNA sequences. <i>Organisms Diversity and Evolution</i>. Springer. <a href=\"https://doi.org/10.1007/s13127-013-0150-6\">https://doi.org/10.1007/s13127-013-0150-6</a>","ama":"Cires Rodriguez E, Baltisberger M, Cuesta C, Vargas P, Prieto J. Allopolyploid origin of the Balkan endemic Ranunculus wettsteinii (Ranunculaceae) inferred from nuclear and plastid DNA sequences. <i>Organisms Diversity and Evolution</i>. 2014;14(1):1-10. doi:<a href=\"https://doi.org/10.1007/s13127-013-0150-6\">10.1007/s13127-013-0150-6</a>","ista":"Cires Rodriguez E, Baltisberger M, Cuesta C, Vargas P, Prieto J. 2014. Allopolyploid origin of the Balkan endemic Ranunculus wettsteinii (Ranunculaceae) inferred from nuclear and plastid DNA sequences. Organisms Diversity and Evolution. 14(1), 1–10.","short":"E. Cires Rodriguez, M. Baltisberger, C. Cuesta, P. Vargas, J. Prieto, Organisms Diversity and Evolution 14 (2014) 1–10.","mla":"Cires Rodriguez, Eduardo, et al. “Allopolyploid Origin of the Balkan Endemic Ranunculus Wettsteinii (Ranunculaceae) Inferred from Nuclear and Plastid DNA Sequences.” <i>Organisms Diversity and Evolution</i>, vol. 14, no. 1, Springer, 2014, pp. 1–10, doi:<a href=\"https://doi.org/10.1007/s13127-013-0150-6\">10.1007/s13127-013-0150-6</a>."},"year":"2014","abstract":[{"lang":"eng","text":"The Balkan Peninsula, characterized by high rates of endemism, is recognised as one of the most diverse and species-rich areas of Europe. However, little is known about the origin of Balkan endemics. The present study addresses the phylogenetic position of the Balkan endemic Ranunculus wettsteinii, as well as its taxonomic status and relationship with the widespread R. parnassiifolius, based on nuclear DNA (internal transcribed spacer, ITS) and plastid regions (rpl32-trnL, rps16-trnQ, trnK-matK and ycf6-psbM). Maximum parsimony and Bayesian inference analyses revealed a well-supported clade formed by accessions of R. wettsteinii. Furthermore, our phylogenetic and network analyses supported previous hypotheses of a likely allopolyploid origin for R. wettsteinii between R. montenegrinus and R. parnassiifolius, with the latter as the maternal parent."}],"publist_id":"4734","doi":"10.1007/s13127-013-0150-6","publication_identifier":{"issn":["14396092"]},"day":"01"},{"publisher":"Cell Press","language":[{"iso":"eng"}],"quality_controlled":"1","page":"691 - 704","intvolume":"       156","title":"The TPLATE adaptor complex drives clathrin-mediated endocytosis in plants","month":"02","date_created":"2018-12-11T11:56:31Z","department":[{"_id":"JiFr"}],"oa_version":"None","publication_status":"published","issue":"4","author":[{"last_name":"Gadeyne","first_name":"Astrid","full_name":"Gadeyne, Astrid"},{"last_name":"Sánchez Rodríguez","first_name":"Clara","full_name":"Sánchez Rodríguez, Clara"},{"last_name":"Vanneste","first_name":"Steffen","full_name":"Vanneste, Steffen"},{"last_name":"Di Rubbo","first_name":"Simone","full_name":"Di Rubbo, Simone"},{"full_name":"Zauber, Henrik","last_name":"Zauber","first_name":"Henrik"},{"full_name":"Vanneste, Kevin","first_name":"Kevin","last_name":"Vanneste"},{"first_name":"Jelle","last_name":"Van Leene","full_name":"Van Leene, Jelle"},{"full_name":"De Winne, Nancy","last_name":"De Winne","first_name":"Nancy"},{"full_name":"Eeckhout, Dominique","last_name":"Eeckhout","first_name":"Dominique"},{"first_name":"Geert","last_name":"Persiau","full_name":"Persiau, Geert"},{"full_name":"Van De Slijke, Eveline","first_name":"Eveline","last_name":"Van De Slijke"},{"last_name":"Cannoot","first_name":"Bernard","full_name":"Cannoot, Bernard"},{"full_name":"Vercruysse, Leen","first_name":"Leen","last_name":"Vercruysse"},{"first_name":"Jonathan","last_name":"Mayers","full_name":"Mayers, Jonathan"},{"id":"45F536D2-F248-11E8-B48F-1D18A9856A87","first_name":"Maciek","last_name":"Adamowski","orcid":"0000-0001-6463-5257","full_name":"Adamowski, Maciek"},{"id":"4AE5C486-F248-11E8-B48F-1D18A9856A87","full_name":"Kania, Urszula","first_name":"Urszula","last_name":"Kania"},{"first_name":"Matthias","last_name":"Ehrlich","full_name":"Ehrlich, Matthias"},{"first_name":"Alois","last_name":"Schweighofer","full_name":"Schweighofer, Alois"},{"first_name":"Tijs","last_name":"Ketelaar","full_name":"Ketelaar, Tijs"},{"last_name":"Maere","first_name":"Steven","full_name":"Maere, Steven"},{"first_name":"Sebastian","last_name":"Bednarek","full_name":"Bednarek, Sebastian"},{"last_name":"Friml","first_name":"Jirí","full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Kris","last_name":"Gevaert","full_name":"Gevaert, Kris"},{"full_name":"Witters, Erwin","last_name":"Witters","first_name":"Erwin"},{"last_name":"Russinova","first_name":"Eugenia","full_name":"Russinova, Eugenia"},{"first_name":"Staffan","last_name":"Persson","full_name":"Persson, Staffan"},{"full_name":"De Jaeger, Geert","first_name":"Geert","last_name":"De Jaeger"},{"first_name":"Daniël","last_name":"Van Damme","full_name":"Van Damme, Daniël"}],"scopus_import":1,"publication":"Cell","_id":"2240","status":"public","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","volume":156,"publist_id":"4721","abstract":[{"lang":"eng","text":"Clathrin-mediated endocytosis is the major mechanism for eukaryotic plasma membrane-based proteome turn-over. In plants, clathrin-mediated endocytosis is essential for physiology and development, but the identification and organization of the machinery operating this process remains largely obscure. Here, we identified an eight-core-component protein complex, the TPLATE complex, essential for plant growth via its role as major adaptor module for clathrin-mediated endocytosis. This complex consists of evolutionarily unique proteins that associate closely with core endocytic elements. The TPLATE complex is recruited as dynamic foci at the plasma membrane preceding recruitment of adaptor protein complex 2, clathrin, and dynamin-related proteins. Reduced function of different complex components severely impaired internalization of assorted endocytic cargoes, demonstrating its pivotal role in clathrin-mediated endocytosis. Taken together, the TPLATE complex is an early endocytic module representing a unique evolutionary plant adaptation of the canonical eukaryotic pathway for clathrin-mediated endocytosis."}],"day":"13","publication_identifier":{"issn":["00928674"]},"doi":"10.1016/j.cell.2014.01.039","type":"journal_article","date_published":"2014-02-13T00:00:00Z","citation":{"ama":"Gadeyne A, Sánchez Rodríguez C, Vanneste S, et al. The TPLATE adaptor complex drives clathrin-mediated endocytosis in plants. <i>Cell</i>. 2014;156(4):691-704. doi:<a href=\"https://doi.org/10.1016/j.cell.2014.01.039\">10.1016/j.cell.2014.01.039</a>","apa":"Gadeyne, A., Sánchez Rodríguez, C., Vanneste, S., Di Rubbo, S., Zauber, H., Vanneste, K., … Van Damme, D. (2014). The TPLATE adaptor complex drives clathrin-mediated endocytosis in plants. <i>Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cell.2014.01.039\">https://doi.org/10.1016/j.cell.2014.01.039</a>","chicago":"Gadeyne, Astrid, Clara Sánchez Rodríguez, Steffen Vanneste, Simone Di Rubbo, Henrik Zauber, Kevin Vanneste, Jelle Van Leene, et al. “The TPLATE Adaptor Complex Drives Clathrin-Mediated Endocytosis in Plants.” <i>Cell</i>. Cell Press, 2014. <a href=\"https://doi.org/10.1016/j.cell.2014.01.039\">https://doi.org/10.1016/j.cell.2014.01.039</a>.","ieee":"A. Gadeyne <i>et al.</i>, “The TPLATE adaptor complex drives clathrin-mediated endocytosis in plants,” <i>Cell</i>, vol. 156, no. 4. Cell Press, pp. 691–704, 2014.","short":"A. Gadeyne, C. Sánchez Rodríguez, S. Vanneste, S. Di Rubbo, H. Zauber, K. Vanneste, J. Van Leene, N. De Winne, D. Eeckhout, G. Persiau, E. Van De Slijke, B. Cannoot, L. Vercruysse, J. Mayers, M. Adamowski, U. Kania, M. Ehrlich, A. Schweighofer, T. Ketelaar, S. Maere, S. Bednarek, J. Friml, K. Gevaert, E. Witters, E. Russinova, S. Persson, G. De Jaeger, D. Van Damme, Cell 156 (2014) 691–704.","mla":"Gadeyne, Astrid, et al. “The TPLATE Adaptor Complex Drives Clathrin-Mediated Endocytosis in Plants.” <i>Cell</i>, vol. 156, no. 4, Cell Press, 2014, pp. 691–704, doi:<a href=\"https://doi.org/10.1016/j.cell.2014.01.039\">10.1016/j.cell.2014.01.039</a>.","ista":"Gadeyne A, Sánchez Rodríguez C, Vanneste S, Di Rubbo S, Zauber H, Vanneste K, Van Leene J, De Winne N, Eeckhout D, Persiau G, Van De Slijke E, Cannoot B, Vercruysse L, Mayers J, Adamowski M, Kania U, Ehrlich M, Schweighofer A, Ketelaar T, Maere S, Bednarek S, Friml J, Gevaert K, Witters E, Russinova E, Persson S, De Jaeger G, Van Damme D. 2014. The TPLATE adaptor complex drives clathrin-mediated endocytosis in plants. Cell. 156(4), 691–704."},"year":"2014","date_updated":"2021-01-12T06:56:13Z"},{"editor":[{"last_name":"Hicks","first_name":"Glenn","full_name":"Hicks, Glenn"},{"full_name":"Robert, Stéphanie","first_name":"Stéphanie","last_name":"Robert"}],"publisher":"Springer","series_title":"Methods in Molecular Biology","quality_controlled":"1","page":"255 - 264","intvolume":"      1056","alternative_title":["Methods in Molecular Biology"],"title":"Analyzing the in vivo status of exogenously applied auxins: A HPLC-based method to characterize the intracellularly localized auxin transporters","department":[{"_id":"JiFr"}],"date_created":"2018-12-11T11:56:32Z","publication_status":"published","author":[{"last_name":"Simon","first_name":"Sibu","full_name":"Simon, Sibu","orcid":"0000-0002-1998-6741","id":"4542EF9A-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Skůpa, Petr","first_name":"Petr","last_name":"Skůpa"},{"last_name":"Dobrev","first_name":"Petre","full_name":"Dobrev, Petre"},{"last_name":"Petrášek","first_name":"Jan","full_name":"Petrášek, Jan"},{"first_name":"Eva","last_name":"Zažímalová","full_name":"Zažímalová, Eva"},{"last_name":"Friml","first_name":"Jirí","full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"scopus_import":1,"_id":"2245","volume":1056,"abstract":[{"lang":"eng","text":"Exogenous application of biologically important molecules for plant growth promotion and/or regulation is very common both in plant research and horticulture. Plant hormones such as auxins and cytokinins are classes of compounds which are often applied exogenously. Nevertheless, plants possess a well-established machinery to regulate the active pool of exogenously applied compounds by converting them to metabolites and conjugates. Consequently, it is often very useful to know the in vivo status of applied compounds to connect them with some of the regulatory events in plant developmental processes. The in vivo status of applied compounds can be measured by incubating plants with radiolabeled compounds, followed by extraction, purification, and HPLC metabolic profiling of plant extracts. Recently we have used this method to characterize the intracellularly localized PIN protein, PIN5. Here we explain the method in detail, with a focus on general application. "}],"day":"01","doi":"10.1007/978-1-62703-592-7_23","year":"2014","citation":{"apa":"Simon, S., Skůpa, P., Dobrev, P., Petrášek, J., Zažímalová, E., &#38; Friml, J. (2014). Analyzing the in vivo status of exogenously applied auxins: A HPLC-based method to characterize the intracellularly localized auxin transporters. In G. Hicks &#38; S. Robert (Eds.), <i>Plant Chemical Genomics</i> (Vol. 1056, pp. 255–264). Springer. <a href=\"https://doi.org/10.1007/978-1-62703-592-7_23\">https://doi.org/10.1007/978-1-62703-592-7_23</a>","ama":"Simon S, Skůpa P, Dobrev P, Petrášek J, Zažímalová E, Friml J. Analyzing the in vivo status of exogenously applied auxins: A HPLC-based method to characterize the intracellularly localized auxin transporters. In: Hicks G, Robert S, eds. <i>Plant Chemical Genomics</i>. Vol 1056. Methods in Molecular Biology. Springer; 2014:255-264. doi:<a href=\"https://doi.org/10.1007/978-1-62703-592-7_23\">10.1007/978-1-62703-592-7_23</a>","chicago":"Simon, Sibu, Petr Skůpa, Petre Dobrev, Jan Petrášek, Eva Zažímalová, and Jiří Friml. “Analyzing the in Vivo Status of Exogenously Applied Auxins: A HPLC-Based Method to Characterize the Intracellularly Localized Auxin Transporters.” In <i>Plant Chemical Genomics</i>, edited by Glenn Hicks and Stéphanie Robert, 1056:255–64. Methods in Molecular Biology. Springer, 2014. <a href=\"https://doi.org/10.1007/978-1-62703-592-7_23\">https://doi.org/10.1007/978-1-62703-592-7_23</a>.","ieee":"S. Simon, P. Skůpa, P. Dobrev, J. Petrášek, E. Zažímalová, and J. Friml, “Analyzing the in vivo status of exogenously applied auxins: A HPLC-based method to characterize the intracellularly localized auxin transporters,” in <i>Plant Chemical Genomics</i>, vol. 1056, G. Hicks and S. Robert, Eds. Springer, 2014, pp. 255–264.","short":"S. Simon, P. Skůpa, P. Dobrev, J. Petrášek, E. Zažímalová, J. Friml, in:, G. Hicks, S. Robert (Eds.), Plant Chemical Genomics, Springer, 2014, pp. 255–264.","mla":"Simon, Sibu, et al. “Analyzing the in Vivo Status of Exogenously Applied Auxins: A HPLC-Based Method to Characterize the Intracellularly Localized Auxin Transporters.” <i>Plant Chemical Genomics</i>, edited by Glenn Hicks and Stéphanie Robert, vol. 1056, Springer, 2014, pp. 255–64, doi:<a href=\"https://doi.org/10.1007/978-1-62703-592-7_23\">10.1007/978-1-62703-592-7_23</a>.","ista":"Simon S, Skůpa P, Dobrev P, Petrášek J, Zažímalová E, Friml J. 2014.Analyzing the in vivo status of exogenously applied auxins: A HPLC-based method to characterize the intracellularly localized auxin transporters. In: Plant Chemical Genomics. Methods in Molecular Biology, vol. 1056, 255–264."},"date_updated":"2021-01-12T06:56:15Z","language":[{"iso":"eng"}],"month":"01","oa_version":"None","publication":"Plant Chemical Genomics","status":"public","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","publist_id":"4704","publication_identifier":{"issn":["10643745"]},"type":"book_chapter","date_published":"2014-01-01T00:00:00Z"},{"citation":{"short":"Y. Chen, K. Aung, J. Rolčík, K. Walicki, J. Friml, F. Brandizzí, Plant Journal 77 (2014) 97–107.","mla":"Chen, Yani, et al. “Inter-Regulation of the Unfolded Protein Response and Auxin Signaling.” <i>Plant Journal</i>, vol. 77, no. 1, Wiley-Blackwell, 2014, pp. 97–107, doi:<a href=\"https://doi.org/10.1111/tpj.12373\">10.1111/tpj.12373</a>.","ista":"Chen Y, Aung K, Rolčík J, Walicki K, Friml J, Brandizzí F. 2014. Inter-regulation of the unfolded protein response and auxin signaling. Plant Journal. 77(1), 97–107.","apa":"Chen, Y., Aung, K., Rolčík, J., Walicki, K., Friml, J., &#38; Brandizzí, F. (2014). Inter-regulation of the unfolded protein response and auxin signaling. <i>Plant Journal</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1111/tpj.12373\">https://doi.org/10.1111/tpj.12373</a>","ama":"Chen Y, Aung K, Rolčík J, Walicki K, Friml J, Brandizzí F. Inter-regulation of the unfolded protein response and auxin signaling. <i>Plant Journal</i>. 2014;77(1):97-107. doi:<a href=\"https://doi.org/10.1111/tpj.12373\">10.1111/tpj.12373</a>","chicago":"Chen, Yani, Kyaw Aung, Jakub Rolčík, Kathryn Walicki, Jiří Friml, and Federica Brandizzí. “Inter-Regulation of the Unfolded Protein Response and Auxin Signaling.” <i>Plant Journal</i>. Wiley-Blackwell, 2014. <a href=\"https://doi.org/10.1111/tpj.12373\">https://doi.org/10.1111/tpj.12373</a>.","ieee":"Y. Chen, K. Aung, J. Rolčík, K. Walicki, J. Friml, and F. Brandizzí, “Inter-regulation of the unfolded protein response and auxin signaling,” <i>Plant Journal</i>, vol. 77, no. 1. Wiley-Blackwell, pp. 97–107, 2014."},"year":"2014","date_updated":"2021-01-12T06:56:17Z","abstract":[{"text":"The unfolded protein response (UPR) is a signaling network triggered by overload of protein-folding demand in the endoplasmic reticulum (ER), a condition termed ER stress. The UPR is critical for growth and development; nonetheless, connections between the UPR and other cellular regulatory processes remain largely unknown. Here, we identify a link between the UPR and the phytohormone auxin, a master regulator of plant physiology. We show that ER stress triggers down-regulation of auxin receptors and transporters in Arabidopsis thaliana. We also demonstrate that an Arabidopsis mutant of a conserved ER stress sensor IRE1 exhibits defects in the auxin response and levels. These data not only support that the plant IRE1 is required for auxin homeostasis, they also reveal a species-specific feature of IRE1 in multicellular eukaryotes. Furthermore, by establishing that UPR activation is reduced in mutants of ER-localized auxin transporters, including PIN5, we define a long-neglected biological significance of ER-based auxin regulation. We further examine the functional relationship of IRE1 and PIN5 by showing that an ire1 pin5 triple mutant enhances defects of UPR activation and auxin homeostasis in ire1 or pin5. Our results imply that the plant UPR has evolved a hormone-dependent strategy for coordinating ER function with physiological processes.","lang":"eng"}],"day":"01","doi":"10.1111/tpj.12373","volume":77,"issue":"1","author":[{"first_name":"Yani","last_name":"Chen","full_name":"Chen, Yani"},{"last_name":"Aung","first_name":"Kyaw","full_name":"Aung, Kyaw"},{"full_name":"Rolčík, Jakub","first_name":"Jakub","last_name":"Rolčík"},{"first_name":"Kathryn","last_name":"Walicki","full_name":"Walicki, Kathryn"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596","last_name":"Friml","first_name":"Jirí"},{"last_name":"Brandizzí","first_name":"Federica","full_name":"Brandizzí, Federica"}],"scopus_import":1,"_id":"2249","intvolume":"        77","title":"Inter-regulation of the unfolded protein response and auxin signaling","department":[{"_id":"JiFr"}],"date_created":"2018-12-11T11:56:34Z","publication_status":"published","quality_controlled":"1","page":"97 - 107","publisher":"Wiley-Blackwell","type":"journal_article","date_published":"2014-01-01T00:00:00Z","oa":1,"publist_id":"4699","publication_identifier":{"issn":["09607412"]},"status":"public","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3981873/","open_access":"1"}],"publication":"Plant Journal","month":"01","oa_version":"Submitted Version","language":[{"iso":"eng"}]},{"volume":77,"date_updated":"2021-01-12T06:56:18Z","year":"2014","citation":{"ista":"Bailly A, Wang B, Zwiewka M, Pollmann S, Schenck D, Lüthen H, Schulz A, Friml J, Geisler M. 2014. Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth. Plant Journal. 77(1), 108–118.","short":"A. Bailly, B. Wang, M. Zwiewka, S. Pollmann, D. Schenck, H. Lüthen, A. Schulz, J. Friml, M. Geisler, Plant Journal 77 (2014) 108–118.","mla":"Bailly, Aurélien, et al. “Expression of TWISTED DWARF1 Lacking Its In-Plane Membrane Anchor Leads to Increased Cell Elongation and Hypermorphic Growth.” <i>Plant Journal</i>, vol. 77, no. 1, Wiley-Blackwell, 2014, pp. 108–18, doi:<a href=\"https://doi.org/10.1111/tpj.12369\">10.1111/tpj.12369</a>.","ieee":"A. Bailly <i>et al.</i>, “Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth,” <i>Plant Journal</i>, vol. 77, no. 1. Wiley-Blackwell, pp. 108–118, 2014.","chicago":"Bailly, Aurélien, Bangjun Wang, Marta Zwiewka, Stephan Pollmann, Daniel Schenck, Hartwig Lüthen, Alexander Schulz, Jiří Friml, and Markus Geisler. “Expression of TWISTED DWARF1 Lacking Its In-Plane Membrane Anchor Leads to Increased Cell Elongation and Hypermorphic Growth.” <i>Plant Journal</i>. Wiley-Blackwell, 2014. <a href=\"https://doi.org/10.1111/tpj.12369\">https://doi.org/10.1111/tpj.12369</a>.","ama":"Bailly A, Wang B, Zwiewka M, et al. Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth. <i>Plant Journal</i>. 2014;77(1):108-118. doi:<a href=\"https://doi.org/10.1111/tpj.12369\">10.1111/tpj.12369</a>","apa":"Bailly, A., Wang, B., Zwiewka, M., Pollmann, S., Schenck, D., Lüthen, H., … Geisler, M. (2014). Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth. <i>Plant Journal</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1111/tpj.12369\">https://doi.org/10.1111/tpj.12369</a>"},"doi":"10.1111/tpj.12369","day":"01","abstract":[{"lang":"eng","text":"Plant growth is achieved predominantly by cellular elongation, which is thought to be controlled on several levels by apoplastic auxin. Auxin export into the apoplast is achieved by plasma membrane efflux catalysts of the PIN-FORMED (PIN) and ATP-binding cassette protein subfamily B/phosphor- glycoprotein (ABCB/PGP) classes; the latter were shown to depend on interaction with the FKBP42, TWISTED DWARF1 (TWD1). Here by using a transgenic approach in combination with phenotypical, biochemical and cell biological analyses we demonstrate the importance of a putative C-terminal in-plane membrane anchor of TWD1 in the regulation of ABCB-mediated auxin transport. In contrast with dwarfed twd1 loss-of-function alleles, TWD1 gain-of-function lines that lack a putative in-plane membrane anchor (HA-TWD1-Ct) show hypermorphic plant architecture, characterized by enhanced stem length and leaf surface but reduced shoot branching. Greater hypocotyl length is the result of enhanced cell elongation that correlates with reduced polar auxin transport capacity for HA-TWD1-Ct. As a consequence, HA-TWD1-Ct displays higher hypocotyl auxin accumulation, which is shown to result in elevated auxin-induced cell elongation rates. Our data highlight the importance of C-terminal membrane anchoring for TWD1 action, which is required for specific regulation of ABCB-mediated auxin transport. These data support a model in which TWD1 controls lateral ABCB1-mediated export into the apoplast, which is required for auxin-mediated cell elongation."}],"page":"108 - 118","quality_controlled":"1","publisher":"Wiley-Blackwell","article_type":"original","_id":"2253","scopus_import":1,"author":[{"first_name":"Aurélien","last_name":"Bailly","full_name":"Bailly, Aurélien"},{"full_name":"Wang, Bangjun","first_name":"Bangjun","last_name":"Wang"},{"first_name":"Marta","last_name":"Zwiewka","full_name":"Zwiewka, Marta"},{"first_name":"Stephan","last_name":"Pollmann","full_name":"Pollmann, Stephan"},{"first_name":"Daniel","last_name":"Schenck","full_name":"Schenck, Daniel"},{"first_name":"Hartwig","last_name":"Lüthen","full_name":"Lüthen, Hartwig"},{"full_name":"Schulz, Alexander","last_name":"Schulz","first_name":"Alexander"},{"orcid":"0000-0002-8302-7596","full_name":"Friml, Jirí","first_name":"Jirí","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Geisler","first_name":"Markus","full_name":"Geisler, Markus"}],"issue":"1","publication_status":"published","article_processing_charge":"No","date_created":"2018-12-11T11:56:35Z","department":[{"_id":"JiFr"}],"title":"Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth","intvolume":"        77","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1111/tpj.12369"}],"status":"public","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_published":"2014-01-01T00:00:00Z","type":"journal_article","publication_identifier":{"issn":["09607412"]},"publist_id":"4694","oa":1,"language":[{"iso":"eng"}],"publication":"Plant Journal","oa_version":"Published Version","project":[{"_id":"256BDAB0-B435-11E9-9278-68D0E5697425","name":"Innovationsförderung in der Grenzregion Österreich – Tschechische Republik durch die Schaffung von Synergien im Bereich der Forschungsinfrastruktur"}],"month":"01"},{"type":"dissertation","date_published":"2014-12-01T00:00:00Z","year":"2014","citation":{"short":"P. Marhavá, Molecular Mechanisms of Patterning and Subcellular Trafficking in Arabidopsis Thaliana, Institute of Science and Technology Austria, 2014.","mla":"Marhavá, Petra. <i>Molecular Mechanisms of Patterning and Subcellular Trafficking in Arabidopsis Thaliana</i>. Institute of Science and Technology Austria, 2014.","ista":"Marhavá P. 2014. Molecular mechanisms of patterning and subcellular trafficking in Arabidopsis thaliana. Institute of Science and Technology Austria.","ama":"Marhavá P. Molecular mechanisms of patterning and subcellular trafficking in Arabidopsis thaliana. 2014.","apa":"Marhavá, P. (2014). <i>Molecular mechanisms of patterning and subcellular trafficking in Arabidopsis thaliana</i>. Institute of Science and Technology Austria.","ieee":"P. Marhavá, “Molecular mechanisms of patterning and subcellular trafficking in Arabidopsis thaliana,” Institute of Science and Technology Austria, 2014.","chicago":"Marhavá, Petra. “Molecular Mechanisms of Patterning and Subcellular Trafficking in Arabidopsis Thaliana.” Institute of Science and Technology Austria, 2014."},"date_updated":"2023-09-07T11:39:38Z","publist_id":"5805","abstract":[{"text":"Phosphatidylinositol (Ptdlns) is a structural phospholipid that can be phosphorylated into various lipid signaling molecules, designated polyphosphoinositides (PPIs). The reversible phosphorylation of PPIs on the 3, 4, or 5 position of inositol is performed by a set of organelle-specific kinases and phosphatases, and the characteristic head groups make these molecules ideal for regulating biological processes in time and space. In yeast and mammals, Ptdlns3P and Ptdlns(3,5)P2 play crucial roles in trafficking toward the lytic compartments, whereas the role in plants is not yet fully understood. Here we identified the role of a land plant-specific subgroup of PPI phosphatases, the suppressor of actin 2 (SAC2) to SAC5, during vauolar trafficking and morphogenesis in Arabidopsis thaliana. SAC2-SAC5 localize to the tonoplast along with Ptdlns3P, the presumable product of their activity. in SAC gain- and loss-of-function mutants, the levels of Ptdlns monophosphates and bisphosphates were changed, with opposite effects on the morphology of storage and lytic vacuoles, and the trafficking toward the vacuoles was defective. Moreover, multiple sac knockout mutants had an increased number of smaller storage and lytic vacuoles, whereas extralarge vacuoles were observed in the overexpression lines, correlating with various growth and developmental defects. The fragmented vacuolar phenotype of sac mutants could be mimicked by treating wild-type seedlings with Ptdlns(3,5)P2, corroborating that this PPI is important for vacuole morphology. Taken together, these results provide evidence that PPIs, together with their metabolic enzymes SAC2-SAC5, are crucial for vacuolar trafficking and for vacuolar morphology and function in plants.","lang":"eng"}],"supervisor":[{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","last_name":"Friml","orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří"}],"day":"01","publication_identifier":{"issn":["2663-337X"]},"degree_awarded":"PhD","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","status":"public","author":[{"first_name":"Petra","last_name":"Marhavá","full_name":"Marhavá, Petra","id":"44E59624-F248-11E8-B48F-1D18A9856A87"}],"_id":"1402","title":"Molecular mechanisms of patterning and subcellular trafficking in Arabidopsis thaliana","month":"12","alternative_title":["ISTA Thesis"],"date_created":"2018-12-11T11:51:49Z","article_processing_charge":"No","department":[{"_id":"JiFr"}],"oa_version":"None","publication_status":"published","language":[{"iso":"eng"}],"page":"90","publisher":"Institute of Science and Technology Austria"},{"year":"2013","citation":{"ama":"Simon S, Kubeš M, Baster P, et al. Defining the selectivity of processes along the auxin response chain: A study using auxin analogues. <i>New Phytologist</i>. 2013;200(4):1034-1048. doi:<a href=\"https://doi.org/10.1111/nph.12437\">10.1111/nph.12437</a>","apa":"Simon, S., Kubeš, M., Baster, P., Robert, S., Dobrev, P., Friml, J., … Zažímalová, E. (2013). Defining the selectivity of processes along the auxin response chain: A study using auxin analogues. <i>New Phytologist</i>. Wiley. <a href=\"https://doi.org/10.1111/nph.12437\">https://doi.org/10.1111/nph.12437</a>","ieee":"S. Simon <i>et al.</i>, “Defining the selectivity of processes along the auxin response chain: A study using auxin analogues,” <i>New Phytologist</i>, vol. 200, no. 4. Wiley, pp. 1034–1048, 2013.","chicago":"Simon, Sibu, Martin Kubeš, Pawel Baster, Stéphanie Robert, Petre Dobrev, Jiří Friml, Jan Petrášek, and Eva Zažímalová. “Defining the Selectivity of Processes along the Auxin Response Chain: A Study Using Auxin Analogues.” <i>New Phytologist</i>. Wiley, 2013. <a href=\"https://doi.org/10.1111/nph.12437\">https://doi.org/10.1111/nph.12437</a>.","mla":"Simon, Sibu, et al. “Defining the Selectivity of Processes along the Auxin Response Chain: A Study Using Auxin Analogues.” <i>New Phytologist</i>, vol. 200, no. 4, Wiley, 2013, pp. 1034–48, doi:<a href=\"https://doi.org/10.1111/nph.12437\">10.1111/nph.12437</a>.","short":"S. Simon, M. Kubeš, P. Baster, S. Robert, P. Dobrev, J. Friml, J. Petrášek, E. Zažímalová, New Phytologist 200 (2013) 1034–1048.","ista":"Simon S, Kubeš M, Baster P, Robert S, Dobrev P, Friml J, Petrášek J, Zažímalová E. 2013. Defining the selectivity of processes along the auxin response chain: A study using auxin analogues. New Phytologist. 200(4), 1034–1048."},"date_updated":"2025-05-07T11:12:32Z","abstract":[{"lang":"eng","text":"The mode of action of auxin is based on its non-uniform distribution within tissues and organs. Despite the wide use of several auxin analogues in research and agriculture, little is known about the specificity of different auxin-related transport and signalling processes towards these compounds. Using seedlings of Arabidopsis thaliana and suspension-cultured cells of Nicotiana tabacum (BY-2), the physiological activity of several auxin analogues was investigated, together with their capacity to induce auxin-dependent gene expression, to inhibit endocytosis and to be transported across the plasma membrane. This study shows that the specificity criteria for different auxin-related processes vary widely. Notably, the special behaviour of some synthetic auxin analogues suggests that they might be useful tools in investigations of the molecular mechanism of auxin action. Thus, due to their differential stimulatory effects on DR5 expression, indole-3-propionic (IPA) and 2,4,5-trichlorophenoxy acetic (2,4,5-T) acids can serve in studies of TRANSPORT INHIBITOR RESPONSE 1/AUXIN SIGNALLING F-BOX (TIR1/AFB)-mediated auxin signalling, and 5-fluoroindole-3-acetic acid (5-F-IAA) can help to discriminate between transcriptional and non-transcriptional pathways of auxin signalling. The results demonstrate that the major determinants for the auxin-like physiological potential of a particular compound are very complex and involve its chemical and metabolic stability, its ability to distribute in tissues in a polar manner and its activity towards auxin signalling machinery."}],"day":"01","doi":"10.1111/nph.12437","volume":200,"acknowledgement":"The authors thank Dr Christian Luschnig (University of Natural Resources and Life Sciences (BOKU), Vienna, Austria) for the anti-PIN2 antibody, Professor Mark Estelle (University of California, San Diego, CA, USA) for tir1-1 mutant seeds and, last but not least, to Dr David Morris for critical reading of the manuscript. We also thank Markéta Pařezová and Jana Stýblová for excellent technical assistance. This work was supported by the Grant Agency of the Czech Republic (P305/11/0797 to E.Z. and 13-40637S to J.F.), the Central European Institute of Technology project CZ.1.05/1.1.00/02.0068 from the European Regional Development Fund and by a European Research Council starting independent research grant ERC-2011-StG-20101109-PSDP (to J.F.).","issue":"4","author":[{"id":"4542EF9A-F248-11E8-B48F-1D18A9856A87","first_name":"Sibu","last_name":"Simon","orcid":"0000-0002-1998-6741","full_name":"Simon, Sibu"},{"first_name":"Martin","last_name":"Kubeš","full_name":"Kubeš, Martin"},{"full_name":"Baster, Pawel","first_name":"Pawel","last_name":"Baster","id":"3028BD74-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Stéphanie","last_name":"Robert","full_name":"Robert, Stéphanie"},{"first_name":"Petre","last_name":"Dobrev","full_name":"Dobrev, Petre"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596","last_name":"Friml","first_name":"Jirí"},{"full_name":"Petrášek, Jan","last_name":"Petrášek","first_name":"Jan"},{"first_name":"Eva","last_name":"Zažímalová","full_name":"Zažímalová, Eva"}],"scopus_import":"1","_id":"2443","intvolume":"       200","title":"Defining the selectivity of processes along the auxin response chain: A study using auxin analogues","date_created":"2018-12-11T11:57:41Z","department":[{"_id":"JiFr"}],"article_processing_charge":"No","publication_status":"published","ec_funded":1,"quality_controlled":"1","page":"1034 - 1048","article_type":"original","publisher":"Wiley","type":"journal_article","date_published":"2013-12-01T00:00:00Z","publist_id":"4460","oa":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","status":"public","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1111/nph.12437"}],"publication":"New Phytologist","month":"12","project":[{"_id":"25716A02-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","name":"Polarity and subcellular dynamics in plants","grant_number":"282300"}],"oa_version":"Published Version","language":[{"iso":"eng"}]},{"external_id":{"pmid":["23857365"]},"date_updated":"2025-05-07T11:12:32Z","year":"2013","citation":{"short":"E. Remy, P. Baster, J. Friml, P. Duque, Plant Signaling &#38; Behavior 8 (2013).","mla":"Remy, Estelle, et al. “ZIFL1.1 Transporter Modulates Polar Auxin Transport by Stabilizing Membrane Abundance of Multiple PINs in Arabidopsis Root Tip.” <i>Plant Signaling &#38; Behavior</i>, vol. 8, no. 10, e25688, Taylor &#38; Francis, 2013, doi:<a href=\"https://doi.org/10.4161/psb.25688\">10.4161/psb.25688</a>.","ista":"Remy E, Baster P, Friml J, Duque P. 2013. ZIFL1.1 transporter modulates polar auxin transport by stabilizing membrane abundance of multiple PINs in Arabidopsis root tip. Plant Signaling &#38; Behavior. 8(10), e25688.","ama":"Remy E, Baster P, Friml J, Duque P. ZIFL1.1 transporter modulates polar auxin transport by stabilizing membrane abundance of multiple PINs in Arabidopsis root tip. <i>Plant Signaling &#38; Behavior</i>. 2013;8(10). doi:<a href=\"https://doi.org/10.4161/psb.25688\">10.4161/psb.25688</a>","apa":"Remy, E., Baster, P., Friml, J., &#38; Duque, P. (2013). ZIFL1.1 transporter modulates polar auxin transport by stabilizing membrane abundance of multiple PINs in Arabidopsis root tip. <i>Plant Signaling &#38; Behavior</i>. Taylor &#38; Francis. <a href=\"https://doi.org/10.4161/psb.25688\">https://doi.org/10.4161/psb.25688</a>","chicago":"Remy, Estelle, Pawel Baster, Jiří Friml, and Paula Duque. “ZIFL1.1 Transporter Modulates Polar Auxin Transport by Stabilizing Membrane Abundance of Multiple PINs in Arabidopsis Root Tip.” <i>Plant Signaling &#38; Behavior</i>. Taylor &#38; Francis, 2013. <a href=\"https://doi.org/10.4161/psb.25688\">https://doi.org/10.4161/psb.25688</a>.","ieee":"E. Remy, P. Baster, J. Friml, and P. Duque, “ZIFL1.1 transporter modulates polar auxin transport by stabilizing membrane abundance of multiple PINs in Arabidopsis root tip,” <i>Plant Signaling &#38; Behavior</i>, vol. 8, no. 10. Taylor &#38; Francis, 2013."},"abstract":[{"lang":"eng","text":"Cell-to-cell directional flow of the phytohormone auxin is primarily established by polar localization of the PIN auxin transporters, a process tightly regulated at multiple levels by auxin itself. We recently reported that, in the context of strong auxin flows, activity of the vacuolar ZIFL1.1 transporter is required for fine-tuning of polar auxin transport rates in the Arabidopsis root. In particular, ZIFL1.1 function protects plasma-membrane stability of the PIN2 carrier in epidermal root tip cells under conditions normally triggering PIN2 degradation. Here, we show that ZIFL1.1 activity at the root tip also promotes PIN1 plasma-membrane abundance in central cylinder cells, thus supporting the notion that ZIFL1.1 acts as a general positive modulator of polar auxin transport in roots."}],"doi":"10.4161/psb.25688","day":"10","volume":8,"author":[{"first_name":"Estelle","last_name":"Remy","full_name":"Remy, Estelle"},{"id":"3028BD74-F248-11E8-B48F-1D18A9856A87","first_name":"Pawel","last_name":"Baster","full_name":"Baster, Pawel"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596","last_name":"Friml","first_name":"Jirí"},{"first_name":"Paula","last_name":"Duque","full_name":"Duque, Paula"}],"issue":"10","pmid":1,"_id":"2448","scopus_import":"1","title":"ZIFL1.1 transporter modulates polar auxin transport by stabilizing membrane abundance of multiple PINs in Arabidopsis root tip","intvolume":"         8","publication_status":"published","date_created":"2018-12-11T11:57:43Z","article_processing_charge":"No","department":[{"_id":"JiFr"}],"ec_funded":1,"quality_controlled":"1","article_type":"original","publisher":"Taylor & Francis","date_published":"2013-07-10T00:00:00Z","type":"journal_article","publist_id":"4455","oa":1,"status":"public","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4091088/","open_access":"1"}],"publication":"Plant Signaling & Behavior","month":"07","article_number":"e25688","oa_version":"Submitted Version","project":[{"call_identifier":"FP7","_id":"25716A02-B435-11E9-9278-68D0E5697425","name":"Polarity and subcellular dynamics in plants","grant_number":"282300"}],"language":[{"iso":"eng"}]},{"language":[{"iso":"eng"}],"page":"1849 - 1862","quality_controlled":"1","publisher":"Cell Press","author":[{"last_name":"Nodzyński","first_name":"Tomasz","full_name":"Nodzyński, Tomasz"},{"last_name":"Feraru","first_name":"Murguel","full_name":"Feraru, Murguel"},{"last_name":"Hirsch","first_name":"Sibylle","full_name":"Hirsch, Sibylle"},{"first_name":"Riet","last_name":"De Rycke","full_name":"De Rycke, Riet"},{"full_name":"Nicuales, Claudiu","first_name":"Claudiu","last_name":"Nicuales"},{"full_name":"Van Leene, Jelle","first_name":"Jelle","last_name":"Van Leene"},{"last_name":"De Jaeger","first_name":"Geert","full_name":"De Jaeger, Geert"},{"last_name":"Vanneste","first_name":"Steffen","full_name":"Vanneste, Steffen"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jirí","last_name":"Friml","orcid":"0000-0002-8302-7596","full_name":"Friml, Jirí"}],"issue":"6","_id":"2449","publication":"Molecular Plant","scopus_import":1,"title":"Retromer subunits VPS35A and VPS29 mediate prevacuolar compartment (PVC) function in Arabidopsis","month":"11","intvolume":"         6","publication_status":"published","oa_version":"None","date_created":"2018-12-11T11:57:44Z","department":[{"_id":"JiFr"}],"status":"public","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","volume":6,"date_published":"2013-11-01T00:00:00Z","type":"journal_article","date_updated":"2021-01-12T06:57:33Z","citation":{"chicago":"Nodzyński, Tomasz, Murguel Feraru, Sibylle Hirsch, Riet De Rycke, Claudiu Nicuales, Jelle Van Leene, Geert De Jaeger, Steffen Vanneste, and Jiří Friml. “Retromer Subunits VPS35A and VPS29 Mediate Prevacuolar Compartment (PVC) Function in Arabidopsis.” <i>Molecular Plant</i>. Cell Press, 2013. <a href=\"https://doi.org/10.1093/mp/sst044\">https://doi.org/10.1093/mp/sst044</a>.","ieee":"T. Nodzyński <i>et al.</i>, “Retromer subunits VPS35A and VPS29 mediate prevacuolar compartment (PVC) function in Arabidopsis,” <i>Molecular Plant</i>, vol. 6, no. 6. Cell Press, pp. 1849–1862, 2013.","ama":"Nodzyński T, Feraru M, Hirsch S, et al. Retromer subunits VPS35A and VPS29 mediate prevacuolar compartment (PVC) function in Arabidopsis. <i>Molecular Plant</i>. 2013;6(6):1849-1862. doi:<a href=\"https://doi.org/10.1093/mp/sst044\">10.1093/mp/sst044</a>","apa":"Nodzyński, T., Feraru, M., Hirsch, S., De Rycke, R., Nicuales, C., Van Leene, J., … Friml, J. (2013). Retromer subunits VPS35A and VPS29 mediate prevacuolar compartment (PVC) function in Arabidopsis. <i>Molecular Plant</i>. Cell Press. <a href=\"https://doi.org/10.1093/mp/sst044\">https://doi.org/10.1093/mp/sst044</a>","ista":"Nodzyński T, Feraru M, Hirsch S, De Rycke R, Nicuales C, Van Leene J, De Jaeger G, Vanneste S, Friml J. 2013. Retromer subunits VPS35A and VPS29 mediate prevacuolar compartment (PVC) function in Arabidopsis. Molecular Plant. 6(6), 1849–1862.","short":"T. Nodzyński, M. Feraru, S. Hirsch, R. De Rycke, C. Nicuales, J. Van Leene, G. De Jaeger, S. Vanneste, J. Friml, Molecular Plant 6 (2013) 1849–1862.","mla":"Nodzyński, Tomasz, et al. “Retromer Subunits VPS35A and VPS29 Mediate Prevacuolar Compartment (PVC) Function in Arabidopsis.” <i>Molecular Plant</i>, vol. 6, no. 6, Cell Press, 2013, pp. 1849–62, doi:<a href=\"https://doi.org/10.1093/mp/sst044\">10.1093/mp/sst044</a>."},"year":"2013","abstract":[{"text":"Intracellular protein routing is mediated by vesicular transport which is tightly regulated in eukaryotes. The protein and lipid homeostasis depends on coordinated delivery of de novo synthesized or recycled cargoes to the plasma membrane by exocytosis and their subsequent removal by rerouting them for recycling or degradation. Here, we report the characterization of protein affected trafficking 3 (pat3) mutant that we identified by an epifluorescence-based forward genetic screen for mutants defective in subcellular distribution of Arabidopsis auxin transporter PIN1–GFP. While pat3 displays largely normal plant morphology and development in nutrient-rich conditions, it shows strong ectopic intracellular accumulations of different plasma membrane cargoes in structures that resemble prevacuolar compartments (PVC) with an aberrant morphology. Genetic mapping revealed that pat3 is defective in vacuolar protein sorting 35A (VPS35A), a putative subunit of the retromer complex that mediates retrograde trafficking between the PVC and trans-Golgi network. Similarly, a mutant defective in another retromer subunit, vps29, shows comparable subcellular defects in PVC morphology and protein accumulation. Thus, our data provide evidence that the retromer components VPS35A and VPS29 are essential for normal PVC morphology and normal trafficking of plasma membrane proteins in plants. In addition, we show that, out of the three VPS35 retromer subunits present in Arabidopsis thaliana genome, the VPS35 homolog A plays a prevailing role in trafficking to the lytic vacuole, presenting another level of complexity in the retromer-dependent vacuolar sorting. ","lang":"eng"}],"publist_id":"4454","doi":"10.1093/mp/sst044","day":"01"},{"language":[{"iso":"eng"}],"has_accepted_license":"1","publication":"PLoS One","article_number":"e70050","month":"07","oa_version":"Published Version","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","status":"public","file":[{"content_type":"application/pdf","file_name":"IST-2016-413-v1+1_journal.pone.0070050.pdf","date_updated":"2020-07-14T12:45:41Z","file_size":2294955,"checksum":"2d47ef47616ef4de1d517d146548184e","date_created":"2018-12-12T10:08:21Z","creator":"system","file_id":"4681","relation":"main_file","access_level":"open_access"}],"type":"journal_article","date_published":"2013-07-23T00:00:00Z","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"publist_id":"4432","oa":1,"file_date_updated":"2020-07-14T12:45:41Z","quality_controlled":"1","publisher":"Public Library of Science","issue":"7","author":[{"full_name":"Čovanová, Milada","last_name":"Čovanová","first_name":"Milada"},{"first_name":"Michael","last_name":"Sauer","full_name":"Sauer, Michael"},{"full_name":"Rychtář, Jan","first_name":"Jan","last_name":"Rychtář"},{"orcid":"0000-0002-8302-7596","full_name":"Friml, Jirí","first_name":"Jirí","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Petrášek, Jan","first_name":"Jan","last_name":"Petrášek"},{"full_name":"Zažímalová, Eva","last_name":"Zažímalová","first_name":"Eva"}],"scopus_import":1,"_id":"2470","intvolume":"         8","title":"Overexpression of the auxin binding PROTEIN1 modulates PIN-dependent auxin transport in tobacco cells","pubrep_id":"413","date_created":"2018-12-11T11:57:51Z","department":[{"_id":"JiFr"}],"publication_status":"published","ddc":["570"],"volume":8,"citation":{"short":"M. Čovanová, M. Sauer, J. Rychtář, J. Friml, J. Petrášek, E. Zažímalová, PLoS One 8 (2013).","mla":"Čovanová, Milada, et al. “Overexpression of the Auxin Binding PROTEIN1 Modulates PIN-Dependent Auxin Transport in Tobacco Cells.” <i>PLoS One</i>, vol. 8, no. 7, e70050, Public Library of Science, 2013, doi:<a href=\"https://doi.org/10.1371/journal.pone.0070050\">10.1371/journal.pone.0070050</a>.","ista":"Čovanová M, Sauer M, Rychtář J, Friml J, Petrášek J, Zažímalová E. 2013. Overexpression of the auxin binding PROTEIN1 modulates PIN-dependent auxin transport in tobacco cells. PLoS One. 8(7), e70050.","ama":"Čovanová M, Sauer M, Rychtář J, Friml J, Petrášek J, Zažímalová E. Overexpression of the auxin binding PROTEIN1 modulates PIN-dependent auxin transport in tobacco cells. <i>PLoS One</i>. 2013;8(7). doi:<a href=\"https://doi.org/10.1371/journal.pone.0070050\">10.1371/journal.pone.0070050</a>","apa":"Čovanová, M., Sauer, M., Rychtář, J., Friml, J., Petrášek, J., &#38; Zažímalová, E. (2013). Overexpression of the auxin binding PROTEIN1 modulates PIN-dependent auxin transport in tobacco cells. <i>PLoS One</i>. Public Library of Science. <a href=\"https://doi.org/10.1371/journal.pone.0070050\">https://doi.org/10.1371/journal.pone.0070050</a>","chicago":"Čovanová, Milada, Michael Sauer, Jan Rychtář, Jiří Friml, Jan Petrášek, and Eva Zažímalová. “Overexpression of the Auxin Binding PROTEIN1 Modulates PIN-Dependent Auxin Transport in Tobacco Cells.” <i>PLoS One</i>. Public Library of Science, 2013. <a href=\"https://doi.org/10.1371/journal.pone.0070050\">https://doi.org/10.1371/journal.pone.0070050</a>.","ieee":"M. Čovanová, M. Sauer, J. Rychtář, J. Friml, J. Petrášek, and E. Zažímalová, “Overexpression of the auxin binding PROTEIN1 modulates PIN-dependent auxin transport in tobacco cells,” <i>PLoS One</i>, vol. 8, no. 7. Public Library of Science, 2013."},"year":"2013","date_updated":"2021-01-12T06:57:40Z","abstract":[{"lang":"eng","text":"Background:Auxin binding protein 1 (ABP1) is a putative auxin receptor and its function is indispensable for plant growth and development. ABP1 has been shown to be involved in auxin-dependent regulation of cell division and expansion, in plasma-membrane-related processes such as changes in transmembrane potential, and in the regulation of clathrin-dependent endocytosis. However, the ABP1-regulated downstream pathway remains elusive.Methodology/Principal Findings:Using auxin transport assays and quantitative analysis of cellular morphology we show that ABP1 regulates auxin efflux from tobacco BY-2 cells. The overexpression of ABP1can counterbalance increased auxin efflux and auxin starvation phenotypes caused by the overexpression of PIN auxin efflux carrier. Relevant mechanism involves the ABP1-controlled vesicle trafficking processes, including positive regulation of endocytosis of PIN auxin efflux carriers, as indicated by fluorescence recovery after photobleaching (FRAP) and pharmacological manipulations.Conclusions/Significance:The findings indicate the involvement of ABP1 in control of rate of auxin transport across plasma membrane emphasizing the role of ABP1 in regulation of PIN activity at the plasma membrane, and highlighting the relevance of ABP1 for the formation of developmentally important, PIN-dependent auxin gradients."}],"day":"23","doi":"10.1371/journal.pone.0070050"},{"language":[{"iso":"eng"}],"project":[{"grant_number":"207362","name":"Hormonal cross-talk in plant organogenesis","_id":"253FCA6A-B435-11E9-9278-68D0E5697425","call_identifier":"FP7"},{"name":"Polarity and subcellular dynamics in plants","grant_number":"282300","call_identifier":"FP7","_id":"25716A02-B435-11E9-9278-68D0E5697425"}],"oa_version":"Published Version","article_number":"e70069","month":"07","has_accepted_license":"1","publication":"PLoS One","file":[{"content_type":"application/pdf","file_name":"IST-2015-393-v1+1_journal.pone.0070069.pdf","date_updated":"2020-07-14T12:45:41Z","checksum":"3be71828b6c2ba9c90eb7056e3f7f57a","file_size":9003465,"date_created":"2018-12-12T10:16:34Z","creator":"system","file_id":"5222","access_level":"open_access","relation":"main_file"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","status":"public","oa":1,"publist_id":"4431","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"type":"journal_article","date_published":"2013-07-29T00:00:00Z","publisher":"Public Library of Science","quality_controlled":"1","ec_funded":1,"file_date_updated":"2020-07-14T12:45:41Z","department":[{"_id":"JiFr"},{"_id":"EvBe"}],"date_created":"2018-12-11T11:57:52Z","publication_status":"published","intvolume":"         8","title":"Role of the Arabidopsis PIN6 auxin transporter in auxin homeostasis and auxin-mediated development","pubrep_id":"393","scopus_import":1,"_id":"2472","issue":"7","author":[{"full_name":"Cazzonelli, Christopher","first_name":"Christopher","last_name":"Cazzonelli"},{"full_name":"Vanstraelen, Marleen","last_name":"Vanstraelen","first_name":"Marleen"},{"id":"4542EF9A-F248-11E8-B48F-1D18A9856A87","last_name":"Simon","first_name":"Sibu","full_name":"Simon, Sibu","orcid":"0000-0002-1998-6741"},{"first_name":"Kuide","last_name":"Yin","full_name":"Yin, Kuide"},{"last_name":"Carron Arthur","first_name":"Ashley","full_name":"Carron Arthur, Ashley"},{"first_name":"Nazia","last_name":"Nisar","full_name":"Nisar, Nazia"},{"first_name":"Gauri","last_name":"Tarle","full_name":"Tarle, Gauri"},{"full_name":"Cuttriss, Abby","first_name":"Abby","last_name":"Cuttriss"},{"full_name":"Searle, Iain","last_name":"Searle","first_name":"Iain"},{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","last_name":"Benková","first_name":"Eva","full_name":"Benková, Eva","orcid":"0000-0002-8510-9739"},{"first_name":"Ulrike","last_name":"Mathesius","full_name":"Mathesius, Ulrike"},{"full_name":"Masle, Josette","first_name":"Josette","last_name":"Masle"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","full_name":"Friml, Jirí","first_name":"Jirí","last_name":"Friml"},{"last_name":"Pogson","first_name":"Barry","full_name":"Pogson, Barry"}],"volume":8,"ddc":["580","570"],"day":"29","doi":"10.1371/journal.pone.0070069","abstract":[{"text":"Plant-specific PIN-formed (PIN) efflux transporters for the plant hormone auxin are required for tissue-specific directional auxin transport and cellular auxin homeostasis. The Arabidopsis PIN protein family has been shown to play important roles in developmental processes such as embryogenesis, organogenesis, vascular tissue differentiation, root meristem patterning and tropic growth. Here we analyzed roles of the less characterised Arabidopsis PIN6 auxin transporter. PIN6 is auxin-inducible and is expressed during multiple auxin-regulated developmental processes. Loss of pin6 function interfered with primary root growth and lateral root development. Misexpression of PIN6 affected auxin transport and interfered with auxin homeostasis in other growth processes such as shoot apical dominance, lateral root primordia development, adventitious root formation, root hair outgrowth and root waving. These changes in auxin-regulated growth correlated with a reduction in total auxin transport as well as with an altered activity of DR5-GUS auxin response reporter. Overall, the data indicate that PIN6 regulates auxin homeostasis during plant development.","lang":"eng"}],"citation":{"chicago":"Cazzonelli, Christopher, Marleen Vanstraelen, Sibu Simon, Kuide Yin, Ashley Carron Arthur, Nazia Nisar, Gauri Tarle, et al. “Role of the Arabidopsis PIN6 Auxin Transporter in Auxin Homeostasis and Auxin-Mediated Development.” <i>PLoS One</i>. Public Library of Science, 2013. <a href=\"https://doi.org/10.1371/journal.pone.0070069\">https://doi.org/10.1371/journal.pone.0070069</a>.","ieee":"C. Cazzonelli <i>et al.</i>, “Role of the Arabidopsis PIN6 auxin transporter in auxin homeostasis and auxin-mediated development,” <i>PLoS One</i>, vol. 8, no. 7. Public Library of Science, 2013.","apa":"Cazzonelli, C., Vanstraelen, M., Simon, S., Yin, K., Carron Arthur, A., Nisar, N., … Pogson, B. (2013). Role of the Arabidopsis PIN6 auxin transporter in auxin homeostasis and auxin-mediated development. <i>PLoS One</i>. Public Library of Science. <a href=\"https://doi.org/10.1371/journal.pone.0070069\">https://doi.org/10.1371/journal.pone.0070069</a>","ama":"Cazzonelli C, Vanstraelen M, Simon S, et al. Role of the Arabidopsis PIN6 auxin transporter in auxin homeostasis and auxin-mediated development. <i>PLoS One</i>. 2013;8(7). doi:<a href=\"https://doi.org/10.1371/journal.pone.0070069\">10.1371/journal.pone.0070069</a>","ista":"Cazzonelli C, Vanstraelen M, Simon S, Yin K, Carron Arthur A, Nisar N, Tarle G, Cuttriss A, Searle I, Benková E, Mathesius U, Masle J, Friml J, Pogson B. 2013. Role of the Arabidopsis PIN6 auxin transporter in auxin homeostasis and auxin-mediated development. PLoS One. 8(7), e70069.","mla":"Cazzonelli, Christopher, et al. “Role of the Arabidopsis PIN6 Auxin Transporter in Auxin Homeostasis and Auxin-Mediated Development.” <i>PLoS One</i>, vol. 8, no. 7, e70069, Public Library of Science, 2013, doi:<a href=\"https://doi.org/10.1371/journal.pone.0070069\">10.1371/journal.pone.0070069</a>.","short":"C. Cazzonelli, M. Vanstraelen, S. Simon, K. Yin, A. Carron Arthur, N. Nisar, G. Tarle, A. Cuttriss, I. Searle, E. Benková, U. Mathesius, J. Masle, J. Friml, B. Pogson, PLoS One 8 (2013)."},"year":"2013","date_updated":"2021-01-12T06:57:41Z"},{"doi":"10.1104/pp.113.214023","day":"03","abstract":[{"lang":"eng","text":"In order to establish a reference for analysis of the function of auxin and the auxin biosynthesis regulators SHORT INTERNODE/ STYLISH (SHI/STY) during Physcomitrella patens reproductive development, we have described male (antheridial) and female (archegonial) development in detail, including temporal and positional information of organ initiation. This has allowed us to define discrete stages of organ morphogenesis and to show that reproductive organ development in P. patens is highly organized and that organ phyllotaxis differs between vegetative and reproductive development. Using the PpSHI1 and PpSHI2 reporter and knockout lines, the auxin reporters GmGH3pro:GUS and PpPINApro:GFP-GUS, and the auxin-conjugating transgene PpSHI2pro:IAAL, we could show that the PpSHI genes, and by inference also auxin, play important roles for reproductive organ development in moss. The PpSHI genes are required for the apical opening of the reproductive organs, the final differentiation of the egg cell, and the progression of canal cells into a cell death program. The apical cells of the archegonium, the canal cells, and the egg cell are also sites of auxin responsiveness and are affected by reduced levels of active auxin, suggesting that auxin mediates PpSHI function in the reproductive organs."}],"date_updated":"2021-01-12T06:59:51Z","citation":{"ama":"Landberg K, Pederson E, Viaene T, et al. The moss physcomitrella patens reproductive organ development is highly organized, affected by the two SHI/STY genes and by the level of active auxin in the SHI/STY expression domain. <i>Plant Physiology</i>. 2013;162(3):1406-1419. doi:<a href=\"https://doi.org/10.1104/pp.113.214023\">10.1104/pp.113.214023</a>","apa":"Landberg, K., Pederson, E., Viaene, T., Bozorg, B., Friml, J., Jönsson, H., … Sundberg, E. (2013). The moss physcomitrella patens reproductive organ development is highly organized, affected by the two SHI/STY genes and by the level of active auxin in the SHI/STY expression domain. <i>Plant Physiology</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1104/pp.113.214023\">https://doi.org/10.1104/pp.113.214023</a>","chicago":"Landberg, Katarina, Eric Pederson, Tom Viaene, Behruz Bozorg, Jiří Friml, Henrik Jönsson, Mattias Thelander, and Eva Sundberg. “The Moss Physcomitrella Patens Reproductive Organ Development Is Highly Organized, Affected by the Two SHI/STY Genes and by the Level of Active Auxin in the SHI/STY Expression Domain.” <i>Plant Physiology</i>. American Society of Plant Biologists, 2013. <a href=\"https://doi.org/10.1104/pp.113.214023\">https://doi.org/10.1104/pp.113.214023</a>.","ieee":"K. Landberg <i>et al.</i>, “The moss physcomitrella patens reproductive organ development is highly organized, affected by the two SHI/STY genes and by the level of active auxin in the SHI/STY expression domain,” <i>Plant Physiology</i>, vol. 162, no. 3. American Society of Plant Biologists, pp. 1406–1419, 2013.","short":"K. Landberg, E. Pederson, T. Viaene, B. Bozorg, J. Friml, H. Jönsson, M. Thelander, E. Sundberg, Plant Physiology 162 (2013) 1406–1419.","mla":"Landberg, Katarina, et al. “The Moss Physcomitrella Patens Reproductive Organ Development Is Highly Organized, Affected by the Two SHI/STY Genes and by the Level of Active Auxin in the SHI/STY Expression Domain.” <i>Plant Physiology</i>, vol. 162, no. 3, American Society of Plant Biologists, 2013, pp. 1406–19, doi:<a href=\"https://doi.org/10.1104/pp.113.214023\">10.1104/pp.113.214023</a>.","ista":"Landberg K, Pederson E, Viaene T, Bozorg B, Friml J, Jönsson H, Thelander M, Sundberg E. 2013. The moss physcomitrella patens reproductive organ development is highly organized, affected by the two SHI/STY genes and by the level of active auxin in the SHI/STY expression domain. Plant Physiology. 162(3), 1406–1419."},"year":"2013","external_id":{"pmid":["23669745"]},"volume":162,"publication_status":"published","department":[{"_id":"JiFr"}],"date_created":"2018-12-11T11:59:42Z","title":"The moss physcomitrella patens reproductive organ development is highly organized, affected by the two SHI/STY genes and by the level of active auxin in the SHI/STY expression domain","intvolume":"       162","pmid":1,"_id":"2808","scopus_import":1,"author":[{"last_name":"Landberg","first_name":"Katarina","full_name":"Landberg, Katarina"},{"last_name":"Pederson","first_name":"Eric","full_name":"Pederson, Eric"},{"last_name":"Viaene","first_name":"Tom","full_name":"Viaene, Tom"},{"full_name":"Bozorg, Behruz","last_name":"Bozorg","first_name":"Behruz"},{"orcid":"0000-0002-8302-7596","full_name":"Friml, Jirí","first_name":"Jirí","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Jönsson, Henrik","first_name":"Henrik","last_name":"Jönsson"},{"first_name":"Mattias","last_name":"Thelander","full_name":"Thelander, Mattias"},{"first_name":"Eva","last_name":"Sundberg","full_name":"Sundberg, Eva"}],"issue":"3","publisher":"American Society of Plant Biologists","page":"1406 - 1419","quality_controlled":"1","oa":1,"publist_id":"4079","date_published":"2013-07-03T00:00:00Z","type":"journal_article","main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3707547/","open_access":"1"}],"status":"public","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","oa_version":"Submitted Version","month":"07","publication":"Plant Physiology","language":[{"iso":"eng"}]},{"publication_status":"published","department":[{"_id":"JiFr"}],"date_created":"2018-12-11T11:59:46Z","title":"A major facilitator superfamily transporter plays a dual role in polar auxin transport and drought stress tolerance in Arabidopsis","intvolume":"        25","_id":"2821","pmid":1,"scopus_import":1,"author":[{"full_name":"Remy, Estelle","first_name":"Estelle","last_name":"Remy"},{"full_name":"Cabrito, Tânia","first_name":"Tânia","last_name":"Cabrito"},{"id":"3028BD74-F248-11E8-B48F-1D18A9856A87","last_name":"Baster","first_name":"Pawel","full_name":"Baster, Pawel"},{"last_name":"Batista","first_name":"Rita","full_name":"Batista, Rita"},{"last_name":"Teixeira","first_name":"Miguel","full_name":"Teixeira, Miguel"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","full_name":"Friml, Jirí","first_name":"Jirí","last_name":"Friml"},{"first_name":"Isabel","last_name":"Sá Correia","full_name":"Sá Correia, Isabel"},{"full_name":"Duque, Paula","last_name":"Duque","first_name":"Paula"}],"issue":"3","publisher":"American Society of Plant Biologists","page":"901 - 926","quality_controlled":"1","doi":"10.1105/tpc.113.110353","day":"24","abstract":[{"text":"Many key aspects of plant development are regulated by the polarized transport of the phytohormone auxin. Cellular auxin efflux, the rate-limiting step in this process, has been shown to rely on the coordinated action of PIN-formed (PIN) and B-type ATP binding cassette (ABCB) carriers. Here, we report that polar auxin transport in the Arabidopsis thaliana root also requires the action of a Major Facilitator Superfamily (MFS) transporter, Zinc-Induced Facilitator-Like 1 (ZIFL1). Sequencing, promoter-reporter, and fluorescent protein fusion experiments indicate that the full-length ZIFL1.1 protein and a truncated splice isoform, ZIFL1.3, localize to the tonoplast of root cells and the plasma membrane of leaf stomatal guard cells, respectively. Using reverse genetics, we show that the ZIFL1.1 transporter regulates various root auxin-related processes, while the ZIFL1.3 isoform mediates drought tolerance by regulating stomatal closure. Auxin transport and immunolocalization assays demonstrate that ZIFL1.1 indirectly modulates cellular auxin efflux during shootward auxin transport at the root tip, likely by regulating plasma membrane PIN2 abundance. Finally, heterologous expression in yeast revealed that ZIFL1.1 and ZIFL1.3 share H+-coupled K+ transport activity. Thus, by determining the subcellular and tissue distribution of two isoforms, alternative splicing dictates a dual function for the ZIFL1 transporter. We propose that this MFS carrier regulates stomatal movements and polar auxin transport by modulating potassium and proton fluxes in Arabidopsis cells.","lang":"eng"}],"date_updated":"2021-01-12T06:59:57Z","citation":{"ieee":"E. Remy <i>et al.</i>, “A major facilitator superfamily transporter plays a dual role in polar auxin transport and drought stress tolerance in Arabidopsis,” <i>Plant Cell</i>, vol. 25, no. 3. American Society of Plant Biologists, pp. 901–926, 2013.","chicago":"Remy, Estelle, Tânia Cabrito, Pawel Baster, Rita Batista, Miguel Teixeira, Jiří Friml, Isabel Sá Correia, and Paula Duque. “A Major Facilitator Superfamily Transporter Plays a Dual Role in Polar Auxin Transport and Drought Stress Tolerance in Arabidopsis.” <i>Plant Cell</i>. American Society of Plant Biologists, 2013. <a href=\"https://doi.org/10.1105/tpc.113.110353\">https://doi.org/10.1105/tpc.113.110353</a>.","apa":"Remy, E., Cabrito, T., Baster, P., Batista, R., Teixeira, M., Friml, J., … Duque, P. (2013). A major facilitator superfamily transporter plays a dual role in polar auxin transport and drought stress tolerance in Arabidopsis. <i>Plant Cell</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1105/tpc.113.110353\">https://doi.org/10.1105/tpc.113.110353</a>","ama":"Remy E, Cabrito T, Baster P, et al. A major facilitator superfamily transporter plays a dual role in polar auxin transport and drought stress tolerance in Arabidopsis. <i>Plant Cell</i>. 2013;25(3):901-926. doi:<a href=\"https://doi.org/10.1105/tpc.113.110353\">10.1105/tpc.113.110353</a>","ista":"Remy E, Cabrito T, Baster P, Batista R, Teixeira M, Friml J, Sá Correia I, Duque P. 2013. A major facilitator superfamily transporter plays a dual role in polar auxin transport and drought stress tolerance in Arabidopsis. Plant Cell. 25(3), 901–926.","short":"E. Remy, T. Cabrito, P. Baster, R. Batista, M. Teixeira, J. Friml, I. Sá Correia, P. Duque, Plant Cell 25 (2013) 901–926.","mla":"Remy, Estelle, et al. “A Major Facilitator Superfamily Transporter Plays a Dual Role in Polar Auxin Transport and Drought Stress Tolerance in Arabidopsis.” <i>Plant Cell</i>, vol. 25, no. 3, American Society of Plant Biologists, 2013, pp. 901–26, doi:<a href=\"https://doi.org/10.1105/tpc.113.110353\">10.1105/tpc.113.110353</a>."},"year":"2013","external_id":{"pmid":["23524662"]},"volume":25,"oa_version":"Submitted Version","month":"04","publication":"Plant Cell","language":[{"iso":"eng"}],"oa":1,"publist_id":"3980","date_published":"2013-04-24T00:00:00Z","type":"journal_article","main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3634696/","open_access":"1"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","status":"public"},{"publist_id":"3972","oa":1,"date_published":"2013-05-07T00:00:00Z","type":"journal_article","status":"public","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","main_file_link":[{"open_access":"1","url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3651428/"}],"month":"05","oa_version":"Submitted Version","project":[{"_id":"2574781E-B435-11E9-9278-68D0E5697425","name":"Koerber Prize 2010"}],"publication":"PNAS","language":[{"iso":"eng"}],"abstract":[{"lang":"eng","text":"Removal of cargos from the cell surface via endocytosis is an efficient mechanism to regulate activities of plasma membrane (PM)-resident proteins, such as receptors or transporters. Salicylic acid (SA) is an important plant hormone that is traditionally associated with pathogen defense. Here, we describe an unanticipated effect of SA on subcellular endocytic cycling of proteins. Both exogenous treatments and endogenously enhanced SA levels repressed endocytosis of different PM proteins. The SA effect on endocytosis did not involve transcription or known components of the SA signaling pathway for transcriptional regulation. SA likely targets an endocytic mechanism that involves the coat protein clathrin, because SA interfered with the clathrin incidence at the PM and clathrin-deficient mutants were less sensitive to the impact of SA on the auxin distribution and root bending during the gravitropic response. By contrast, SA did not affect the ligand-induced endocytosis of the FLAGELLIN SENSING2 (FLS2) receptor during pathogen responses. Our data suggest that the established SA impact on transcription in plant immunity and the nontranscriptional effect of SA on clathrin-mediated endocytosis are independent mechanisms by which SA regulates distinct aspects of plant physiology."}],"doi":"10.1073/pnas.1220205110","day":"07","external_id":{"pmid":["23613581"]},"date_updated":"2021-01-12T06:59:59Z","citation":{"ama":"Du Y, Tejos R, Beck M, et al. Salicylic acid interferes with clathrin-mediated endocytic protein trafficking. <i>PNAS</i>. 2013;110(19):7946-7951. doi:<a href=\"https://doi.org/10.1073/pnas.1220205110\">10.1073/pnas.1220205110</a>","apa":"Du, Y., Tejos, R., Beck, M., Himschoot, E., Li, H., Robatzek, S., … Friml, J. (2013). Salicylic acid interferes with clathrin-mediated endocytic protein trafficking. <i>PNAS</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.1220205110\">https://doi.org/10.1073/pnas.1220205110</a>","ieee":"Y. Du <i>et al.</i>, “Salicylic acid interferes with clathrin-mediated endocytic protein trafficking,” <i>PNAS</i>, vol. 110, no. 19. National Academy of Sciences, pp. 7946–7951, 2013.","chicago":"Du, Yunlong, Ricardo Tejos, Martina Beck, Ellie Himschoot, Hongjiang Li, Silke Robatzek, Steffen Vanneste, and Jiří Friml. “Salicylic Acid Interferes with Clathrin-Mediated Endocytic Protein Trafficking.” <i>PNAS</i>. National Academy of Sciences, 2013. <a href=\"https://doi.org/10.1073/pnas.1220205110\">https://doi.org/10.1073/pnas.1220205110</a>.","mla":"Du, Yunlong, et al. “Salicylic Acid Interferes with Clathrin-Mediated Endocytic Protein Trafficking.” <i>PNAS</i>, vol. 110, no. 19, National Academy of Sciences, 2013, pp. 7946–51, doi:<a href=\"https://doi.org/10.1073/pnas.1220205110\">10.1073/pnas.1220205110</a>.","short":"Y. Du, R. Tejos, M. Beck, E. Himschoot, H. Li, S. Robatzek, S. Vanneste, J. Friml, PNAS 110 (2013) 7946–7951.","ista":"Du Y, Tejos R, Beck M, Himschoot E, Li H, Robatzek S, Vanneste S, Friml J. 2013. Salicylic acid interferes with clathrin-mediated endocytic protein trafficking. PNAS. 110(19), 7946–7951."},"year":"2013","volume":110,"title":"Salicylic acid interferes with clathrin-mediated endocytic protein trafficking","intvolume":"       110","publication_status":"published","date_created":"2018-12-11T11:59:48Z","department":[{"_id":"JiFr"}],"author":[{"last_name":"Du","first_name":"Yunlong","full_name":"Du, Yunlong"},{"full_name":"Tejos, Ricardo","first_name":"Ricardo","last_name":"Tejos"},{"first_name":"Martina","last_name":"Beck","full_name":"Beck, Martina"},{"last_name":"Himschoot","first_name":"Ellie","full_name":"Himschoot, Ellie"},{"full_name":"Li, Hongjiang","orcid":"0000-0001-5039-9660","last_name":"Li","first_name":"Hongjiang","id":"33CA54A6-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Silke","last_name":"Robatzek","full_name":"Robatzek, Silke"},{"last_name":"Vanneste","first_name":"Steffen","full_name":"Vanneste, Steffen"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","first_name":"Jirí","full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596"}],"issue":"19","pmid":1,"_id":"2827","scopus_import":1,"publisher":"National Academy of Sciences","page":"7946 - 7951","quality_controlled":"1"},{"_id":"2832","scopus_import":1,"author":[{"full_name":"Tanaka, Hirokazu","last_name":"Tanaka","first_name":"Hirokazu"},{"full_name":"Kitakura, Saeko","last_name":"Kitakura","first_name":"Saeko"},{"full_name":"Rakusová, Hana","last_name":"Rakusová","first_name":"Hana"},{"full_name":"Uemura, Tomohiro","first_name":"Tomohiro","last_name":"Uemura"},{"last_name":"Feraru","first_name":"Mugurel","full_name":"Feraru, Mugurel"},{"full_name":"De Rycke, Riet","last_name":"De Rycke","first_name":"Riet"},{"last_name":"Robert","first_name":"Stéphanie","full_name":"Robert, Stéphanie"},{"full_name":"Kakimoto, Tatsuo","last_name":"Kakimoto","first_name":"Tatsuo"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","full_name":"Friml, Jirí","first_name":"Jirí","last_name":"Friml"}],"issue":"5","publication_status":"published","date_created":"2018-12-11T11:59:50Z","department":[{"_id":"JiFr"}],"pubrep_id":"411","title":"Cell polarity and patterning by PIN trafficking through early endosomal compartments in arabidopsis thaliana","intvolume":"         9","ec_funded":1,"quality_controlled":"1","file_date_updated":"2020-07-14T12:45:50Z","publisher":"Public Library of Science","date_updated":"2021-01-12T07:00:03Z","year":"2013","citation":{"ista":"Tanaka H, Kitakura S, Rakusová H, Uemura T, Feraru M, De Rycke R, Robert S, Kakimoto T, Friml J. 2013. Cell polarity and patterning by PIN trafficking through early endosomal compartments in arabidopsis thaliana. PLoS Genetics. 9(5), e1003540.","mla":"Tanaka, Hirokazu, et al. “Cell Polarity and Patterning by PIN Trafficking through Early Endosomal Compartments in Arabidopsis Thaliana.” <i>PLoS Genetics</i>, vol. 9, no. 5, e1003540, Public Library of Science, 2013, doi:<a href=\"https://doi.org/10.1371/journal.pgen.1003540\">10.1371/journal.pgen.1003540</a>.","short":"H. Tanaka, S. Kitakura, H. Rakusová, T. Uemura, M. Feraru, R. De Rycke, S. Robert, T. Kakimoto, J. Friml, PLoS Genetics 9 (2013).","chicago":"Tanaka, Hirokazu, Saeko Kitakura, Hana Rakusová, Tomohiro Uemura, Mugurel Feraru, Riet De Rycke, Stéphanie Robert, Tatsuo Kakimoto, and Jiří Friml. “Cell Polarity and Patterning by PIN Trafficking through Early Endosomal Compartments in Arabidopsis Thaliana.” <i>PLoS Genetics</i>. Public Library of Science, 2013. <a href=\"https://doi.org/10.1371/journal.pgen.1003540\">https://doi.org/10.1371/journal.pgen.1003540</a>.","ieee":"H. Tanaka <i>et al.</i>, “Cell polarity and patterning by PIN trafficking through early endosomal compartments in arabidopsis thaliana,” <i>PLoS Genetics</i>, vol. 9, no. 5. Public Library of Science, 2013.","ama":"Tanaka H, Kitakura S, Rakusová H, et al. Cell polarity and patterning by PIN trafficking through early endosomal compartments in arabidopsis thaliana. <i>PLoS Genetics</i>. 2013;9(5). doi:<a href=\"https://doi.org/10.1371/journal.pgen.1003540\">10.1371/journal.pgen.1003540</a>","apa":"Tanaka, H., Kitakura, S., Rakusová, H., Uemura, T., Feraru, M., De Rycke, R., … Friml, J. (2013). Cell polarity and patterning by PIN trafficking through early endosomal compartments in arabidopsis thaliana. <i>PLoS Genetics</i>. Public Library of Science. <a href=\"https://doi.org/10.1371/journal.pgen.1003540\">https://doi.org/10.1371/journal.pgen.1003540</a>"},"doi":"10.1371/journal.pgen.1003540","day":"05","abstract":[{"text":"PIN-FORMED (PIN) proteins localize asymmetrically at the plasma membrane and mediate intercellular polar transport of the plant hormone auxin that is crucial for a multitude of developmental processes in plants. PIN localization is under extensive control by environmental or developmental cues, but mechanisms regulating PIN localization are not fully understood. Here we show that early endosomal components ARF GEF BEN1 and newly identified Sec1/Munc18 family protein BEN2 are involved in distinct steps of early endosomal trafficking. BEN1 and BEN2 are collectively required for polar PIN localization, for their dynamic repolarization, and consequently for auxin activity gradient formation and auxin-related developmental processes including embryonic patterning, organogenesis, and vasculature venation patterning. These results show that early endosomal trafficking is crucial for cell polarity and auxin-dependent regulation of plant architecture.","lang":"eng"}],"volume":9,"ddc":["570"],"publication":"PLoS Genetics","has_accepted_license":"1","oa_version":"Published Version","project":[{"grant_number":"282300","name":"Polarity and subcellular dynamics in plants","_id":"25716A02-B435-11E9-9278-68D0E5697425","call_identifier":"FP7"}],"month":"05","article_number":"e1003540","language":[{"iso":"eng"}],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"date_published":"2013-05-05T00:00:00Z","type":"journal_article","publist_id":"3967","oa":1,"file":[{"file_id":"4957","creator":"system","access_level":"open_access","relation":"main_file","date_updated":"2020-07-14T12:45:50Z","file_name":"IST-2016-411-v1+1_journal.pgen.1003540.pdf","content_type":"application/pdf","date_created":"2018-12-12T10:12:39Z","file_size":3813091,"checksum":"050237d6c53e8d1601b26808ee1dd6d8"}],"status":"public","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87"},{"oa":1,"publist_id":"3964","type":"journal_article","date_published":"2013-06-01T00:00:00Z","status":"public","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3668084/","open_access":"1"}],"month":"06","oa_version":"Submitted Version","publication":"Plant Physiology","language":[{"iso":"eng"}],"abstract":[{"text":"The phytohormone auxin regulates virtually every aspect of plant development. To identify new genes involved in auxin activity, a genetic screen was performed for Arabidopsis (Arabidopsis thaliana) mutants with altered expression of the auxin-responsive reporter DR5rev:GFP. One of the mutants recovered in the screen, designated as weak auxin response3 (wxr3), exhibits much lower DR5rev:GFP expression when treated with the synthetic auxin 2,4-dichlorophenoxyacetic acid and displays severe defects in root development. The wxr3 mutant decreases polar auxin transport and results in a disruption of the asymmetric auxin distribution. The levels of the auxin transporters AUXIN1 and PIN-FORMED are dramatically reduced in the wxr3 root tip. Molecular analyses demonstrate that WXR3 is ROOT ULTRAVIOLET B-SENSITIVE1 (RUS1), a member of the conserved Domain of Unknown Function647 protein family found in diverse eukaryotic organisms. Our data suggest that RUS1/WXR3 plays an essential role in the regulation of polar auxin transport by maintaining the proper level of auxin transporters on the plasma membrane.","lang":"eng"}],"day":"01","doi":"10.1104/pp.113.217018","external_id":{"pmid":["23580592"]},"year":"2013","citation":{"short":"H. Yu, M. Karampelias, S. Robert, W. Peer, R. Swarup, S. Ye, L. Ge, J. Cohen, A. Murphy, J. Friml, M. Estelle, Plant Physiology 162 (2013) 965–976.","mla":"Yu, Hong, et al. “Root Ultraviolet B-Sensitive1/Weak Auxin Response3 Is Essential for Polar Auxin Transport in Arabidopsis.” <i>Plant Physiology</i>, vol. 162, no. 2, American Society of Plant Biologists, 2013, pp. 965–76, doi:<a href=\"https://doi.org/10.1104/pp.113.217018\">10.1104/pp.113.217018</a>.","ista":"Yu H, Karampelias M, Robert S, Peer W, Swarup R, Ye S, Ge L, Cohen J, Murphy A, Friml J, Estelle M. 2013. Root ultraviolet b-sensitive1/weak auxin response3 is essential for polar auxin transport in arabidopsis. Plant Physiology. 162(2), 965–976.","apa":"Yu, H., Karampelias, M., Robert, S., Peer, W., Swarup, R., Ye, S., … Estelle, M. (2013). Root ultraviolet b-sensitive1/weak auxin response3 is essential for polar auxin transport in arabidopsis. <i>Plant Physiology</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1104/pp.113.217018\">https://doi.org/10.1104/pp.113.217018</a>","ama":"Yu H, Karampelias M, Robert S, et al. Root ultraviolet b-sensitive1/weak auxin response3 is essential for polar auxin transport in arabidopsis. <i>Plant Physiology</i>. 2013;162(2):965-976. doi:<a href=\"https://doi.org/10.1104/pp.113.217018\">10.1104/pp.113.217018</a>","chicago":"Yu, Hong, Michael Karampelias, Stéphanie Robert, Wendy Peer, Ranjan Swarup, Songqing Ye, Lei Ge, et al. “Root Ultraviolet B-Sensitive1/Weak Auxin Response3 Is Essential for Polar Auxin Transport in Arabidopsis.” <i>Plant Physiology</i>. American Society of Plant Biologists, 2013. <a href=\"https://doi.org/10.1104/pp.113.217018\">https://doi.org/10.1104/pp.113.217018</a>.","ieee":"H. Yu <i>et al.</i>, “Root ultraviolet b-sensitive1/weak auxin response3 is essential for polar auxin transport in arabidopsis,” <i>Plant Physiology</i>, vol. 162, no. 2. American Society of Plant Biologists, pp. 965–976, 2013."},"date_updated":"2021-01-12T07:00:05Z","volume":162,"intvolume":"       162","title":"Root ultraviolet b-sensitive1/weak auxin response3 is essential for polar auxin transport in arabidopsis","department":[{"_id":"JiFr"}],"date_created":"2018-12-11T11:59:51Z","publication_status":"published","issue":"2","author":[{"full_name":"Yu, Hong","first_name":"Hong","last_name":"Yu"},{"full_name":"Karampelias, Michael","last_name":"Karampelias","first_name":"Michael"},{"last_name":"Robert","first_name":"Stéphanie","full_name":"Robert, Stéphanie"},{"full_name":"Peer, Wendy","last_name":"Peer","first_name":"Wendy"},{"full_name":"Swarup, Ranjan","first_name":"Ranjan","last_name":"Swarup"},{"last_name":"Ye","first_name":"Songqing","full_name":"Ye, Songqing"},{"first_name":"Lei","last_name":"Ge","full_name":"Ge, Lei"},{"full_name":"Cohen, Jerry","first_name":"Jerry","last_name":"Cohen"},{"full_name":"Murphy, Angus","first_name":"Angus","last_name":"Murphy"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","full_name":"Friml, Jirí","first_name":"Jirí","last_name":"Friml"},{"full_name":"Estelle, Mark","first_name":"Mark","last_name":"Estelle"}],"scopus_import":1,"pmid":1,"_id":"2835","publisher":"American Society of Plant Biologists","quality_controlled":"1","page":"965 - 976"},{"oa_version":"None","publication_status":"published","date_created":"2018-12-11T11:59:53Z","department":[{"_id":"JiFr"},{"_id":"EvBe"}],"project":[{"_id":"253FCA6A-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","grant_number":"207362","name":"Hormonal cross-talk in plant organogenesis"}],"title":"An auxin transport mechanism restricts positive orthogravitropism in lateral roots","month":"05","intvolume":"        23","publication":"Current Biology","_id":"2844","scopus_import":1,"author":[{"full_name":"Rosquete, Michel","last_name":"Rosquete","first_name":"Michel"},{"id":"49E91952-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6862-1247","full_name":"Von Wangenheim, Daniel","first_name":"Daniel","last_name":"Von Wangenheim"},{"first_name":"Peter","last_name":"Marhavy","orcid":"0000-0001-5227-5741","full_name":"Marhavy, Peter","id":"3F45B078-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Elke","last_name":"Barbez","full_name":"Barbez, Elke"},{"full_name":"Stelzer, Ernst","last_name":"Stelzer","first_name":"Ernst"},{"first_name":"Eva","last_name":"Benková","orcid":"0000-0002-8510-9739","full_name":"Benková, Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Maizel, Alexis","first_name":"Alexis","last_name":"Maizel"},{"last_name":"Kleine Vehn","first_name":"Jürgen","full_name":"Kleine Vehn, Jürgen"}],"issue":"9","publisher":"Cell Press","page":"817 - 822","ec_funded":1,"quality_controlled":"1","language":[{"iso":"eng"}],"doi":"10.1016/j.cub.2013.03.064","day":"06","abstract":[{"lang":"eng","text":"As soon as a seed germinates, plant growth relates to gravity to ensure that the root penetrates the soil and the shoot expands aerially. Whereas mechanisms of positive and negative orthogravitropism of primary roots and shoots are relatively well understood [1-3], lateral organs often show more complex growth behavior [4]. Lateral roots (LRs) seemingly suppress positive gravitropic growth and show a defined gravitropic set-point angle (GSA) that allows radial expansion of the root system (plagiotropism) [3, 4]. Despite its eminent importance for root architecture, it so far remains completely unknown how lateral organs partially suppress positive orthogravitropism. Here we show that the phytohormone auxin steers GSA formation and limits positive orthogravitropism in LR. Low and high auxin levels/signaling lead to radial or axial root systems, respectively. At a cellular level, it is the auxin transport-dependent regulation of asymmetric growth in the elongation zone that determines GSA. Our data suggest that strong repression of PIN4/PIN7 and transient PIN3 expression limit auxin redistribution in young LR columella cells. We conclude that PIN activity, by temporally limiting the asymmetric auxin fluxes in the tip of LRs, induces transient, differential growth responses in the elongation zone and, consequently, controls root architecture."}],"publist_id":"3950","date_updated":"2021-01-12T07:00:10Z","year":"2013","citation":{"apa":"Rosquete, M., von Wangenheim, D., Marhavý, P., Barbez, E., Stelzer, E., Benková, E., … Kleine Vehn, J. (2013). An auxin transport mechanism restricts positive orthogravitropism in lateral roots. <i>Current Biology</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cub.2013.03.064\">https://doi.org/10.1016/j.cub.2013.03.064</a>","ama":"Rosquete M, von Wangenheim D, Marhavý P, et al. An auxin transport mechanism restricts positive orthogravitropism in lateral roots. <i>Current Biology</i>. 2013;23(9):817-822. doi:<a href=\"https://doi.org/10.1016/j.cub.2013.03.064\">10.1016/j.cub.2013.03.064</a>","chicago":"Rosquete, Michel, Daniel von Wangenheim, Peter Marhavý, Elke Barbez, Ernst Stelzer, Eva Benková, Alexis Maizel, and Jürgen Kleine Vehn. “An Auxin Transport Mechanism Restricts Positive Orthogravitropism in Lateral Roots.” <i>Current Biology</i>. Cell Press, 2013. <a href=\"https://doi.org/10.1016/j.cub.2013.03.064\">https://doi.org/10.1016/j.cub.2013.03.064</a>.","ieee":"M. Rosquete <i>et al.</i>, “An auxin transport mechanism restricts positive orthogravitropism in lateral roots,” <i>Current Biology</i>, vol. 23, no. 9. Cell Press, pp. 817–822, 2013.","short":"M. Rosquete, D. von Wangenheim, P. Marhavý, E. Barbez, E. Stelzer, E. Benková, A. Maizel, J. Kleine Vehn, Current Biology 23 (2013) 817–822.","mla":"Rosquete, Michel, et al. “An Auxin Transport Mechanism Restricts Positive Orthogravitropism in Lateral Roots.” <i>Current Biology</i>, vol. 23, no. 9, Cell Press, 2013, pp. 817–22, doi:<a href=\"https://doi.org/10.1016/j.cub.2013.03.064\">10.1016/j.cub.2013.03.064</a>.","ista":"Rosquete M, von Wangenheim D, Marhavý P, Barbez E, Stelzer E, Benková E, Maizel A, Kleine Vehn J. 2013. An auxin transport mechanism restricts positive orthogravitropism in lateral roots. Current Biology. 23(9), 817–822."},"date_published":"2013-05-06T00:00:00Z","type":"journal_article","volume":23,"status":"public","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87"}]