[{"external_id":{"pmid":["29525951"]},"scopus_import":"1","date_updated":"2021-01-12T07:54:21Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication_identifier":{"issn":["1064-3745"]},"publist_id":"7421","oa_version":"None","year":"2018","volume":1761,"publication_status":"published","date_published":"2018-03-01T00:00:00Z","_id":"408","abstract":[{"lang":"eng","text":"Adventitious roots (AR) are de novo formed roots that emerge from any part of the plant or from callus in tissue culture, except root tissue. The plant tissue origin and the method by which they are induced determine the physiological properties of emerged ARs. Hence, a standard method encompassing all types of AR does not exist. Here we describe a method for the induction and analysis of AR that emerge from the etiolated hypocotyl of dicot plants. The hypocotyl is formed during embryogenesis and shows a determined developmental pattern which usually does not involve AR formation. However, the hypocotyl shows propensity to form de novo roots under specific circumstances such as removal of the root system, high humidity or flooding, or during de-etiolation. The hypocotyl AR emerge from a pericycle-like cell layer surrounding the vascular tissue of the central cylinder, which is reminiscent to the developmental program of lateral roots. Here we propose an easy protocol for in vitro hypocotyl AR induction from etiolated Arabidopsis seedlings."}],"article_processing_charge":"No","language":[{"iso":"eng"}],"doi":"10.1007/978-1-4939-7747-5_7","pmid":1,"day":"01","type":"book_chapter","author":[{"last_name":"Trinh","full_name":"Trinh, Hoang","first_name":"Hoang"},{"id":"362BF7FE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-7241-2328","last_name":"Verstraeten","full_name":"Verstraeten, Inge","first_name":"Inge"},{"first_name":"Danny","full_name":"Geelen, Danny","last_name":"Geelen"}],"alternative_title":["MIMB"],"citation":{"ama":"Trinh H, Verstraeten I, Geelen D. In vitro assay for induction of adventitious rooting on intact arabidopsis hypocotyls. In: <i>Root Development </i>. Vol 1761. Springer Nature; 2018:95-102. doi:<a href=\"https://doi.org/10.1007/978-1-4939-7747-5_7\">10.1007/978-1-4939-7747-5_7</a>","apa":"Trinh, H., Verstraeten, I., &#38; Geelen, D. (2018). In vitro assay for induction of adventitious rooting on intact arabidopsis hypocotyls. In <i>Root Development </i> (Vol. 1761, pp. 95–102). Springer Nature. <a href=\"https://doi.org/10.1007/978-1-4939-7747-5_7\">https://doi.org/10.1007/978-1-4939-7747-5_7</a>","short":"H. Trinh, I. Verstraeten, D. Geelen, in:, Root Development , Springer Nature, 2018, pp. 95–102.","mla":"Trinh, Hoang, et al. “In Vitro Assay for Induction of Adventitious Rooting on Intact Arabidopsis Hypocotyls.” <i>Root Development </i>, vol. 1761, Springer Nature, 2018, pp. 95–102, doi:<a href=\"https://doi.org/10.1007/978-1-4939-7747-5_7\">10.1007/978-1-4939-7747-5_7</a>.","chicago":"Trinh, Hoang, Inge Verstraeten, and Danny Geelen. “In Vitro Assay for Induction of Adventitious Rooting on Intact Arabidopsis Hypocotyls.” In <i>Root Development </i>, 1761:95–102. Springer Nature, 2018. <a href=\"https://doi.org/10.1007/978-1-4939-7747-5_7\">https://doi.org/10.1007/978-1-4939-7747-5_7</a>.","ista":"Trinh H, Verstraeten I, Geelen D. 2018.In vitro assay for induction of adventitious rooting on intact arabidopsis hypocotyls. In: Root Development . MIMB, vol. 1761, 95–102.","ieee":"H. Trinh, I. Verstraeten, and D. Geelen, “In vitro assay for induction of adventitious rooting on intact arabidopsis hypocotyls,” in <i>Root Development </i>, vol. 1761, Springer Nature, 2018, pp. 95–102."},"title":"In vitro assay for induction of adventitious rooting on intact arabidopsis hypocotyls","publication":"Root Development ","department":[{"_id":"JiFr"}],"quality_controlled":"1","intvolume":"      1761","status":"public","publisher":"Springer Nature","month":"03","date_created":"2018-12-11T11:46:18Z","page":"95 - 102"},{"date_updated":"2021-01-12T07:54:34Z","language":[{"iso":"eng"}],"user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","scopus_import":1,"doi":"10.1007/978-1-4939-7747-5_10","day":"11","publist_id":"7418","year":"2018","alternative_title":["Methods in Molecular Biology"],"oa_version":"None","type":"book_chapter","author":[{"full_name":"Karampelias, Michael","first_name":"Michael","last_name":"Karampelias"},{"first_name":"Ricardo","full_name":"Tejos, Ricardo","last_name":"Tejos"},{"last_name":"Friml","first_name":"Jirí","full_name":"Friml, Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596"},{"last_name":"Vanneste","first_name":"Steffen","full_name":"Vanneste, Steffen"}],"citation":{"mla":"Karampelias, Michael, et al. “Optimized Whole Mount in Situ Immunolocalization for Arabidopsis Thaliana  Root Meristems and Lateral Root Primordia.” <i>Root Development. Methods and Protocols</i>, edited by Daniela Ristova and Elke Barbez, vol. 1761, Springer, 2018, pp. 131–43, doi:<a href=\"https://doi.org/10.1007/978-1-4939-7747-5_10\">10.1007/978-1-4939-7747-5_10</a>.","ista":"Karampelias M, Tejos R, Friml J, Vanneste S. 2018.Optimized whole mount in situ immunolocalization for Arabidopsis thaliana  root meristems and lateral root primordia. In: Root Development. Methods and Protocols. Methods in Molecular Biology, vol. 1761, 131–143.","ieee":"M. Karampelias, R. Tejos, J. Friml, and S. Vanneste, “Optimized whole mount in situ immunolocalization for Arabidopsis thaliana  root meristems and lateral root primordia,” in <i>Root Development. Methods and Protocols</i>, vol. 1761, D. Ristova and E. Barbez, Eds. Springer, 2018, pp. 131–143.","chicago":"Karampelias, Michael, Ricardo Tejos, Jiří Friml, and Steffen Vanneste. “Optimized Whole Mount in Situ Immunolocalization for Arabidopsis Thaliana  Root Meristems and Lateral Root Primordia.” In <i>Root Development. Methods and Protocols</i>, edited by Daniela Ristova and Elke Barbez, 1761:131–43. MIMB. Springer, 2018. <a href=\"https://doi.org/10.1007/978-1-4939-7747-5_10\">https://doi.org/10.1007/978-1-4939-7747-5_10</a>.","apa":"Karampelias, M., Tejos, R., Friml, J., &#38; Vanneste, S. (2018). Optimized whole mount in situ immunolocalization for Arabidopsis thaliana  root meristems and lateral root primordia. In D. Ristova &#38; E. Barbez (Eds.), <i>Root Development. Methods and Protocols</i> (Vol. 1761, pp. 131–143). Springer. <a href=\"https://doi.org/10.1007/978-1-4939-7747-5_10\">https://doi.org/10.1007/978-1-4939-7747-5_10</a>","ama":"Karampelias M, Tejos R, Friml J, Vanneste S. Optimized whole mount in situ immunolocalization for Arabidopsis thaliana  root meristems and lateral root primordia. In: Ristova D, Barbez E, eds. <i>Root Development. Methods and Protocols</i>. Vol 1761. MIMB. Springer; 2018:131-143. doi:<a href=\"https://doi.org/10.1007/978-1-4939-7747-5_10\">10.1007/978-1-4939-7747-5_10</a>","short":"M. Karampelias, R. Tejos, J. Friml, S. Vanneste, in:, D. Ristova, E. Barbez (Eds.), Root Development. Methods and Protocols, Springer, 2018, pp. 131–143."},"editor":[{"last_name":"Ristova","first_name":"Daniela","full_name":"Ristova, Daniela"},{"first_name":"Elke","full_name":"Barbez, Elke","last_name":"Barbez"}],"title":"Optimized whole mount in situ immunolocalization for Arabidopsis thaliana  root meristems and lateral root primordia","department":[{"_id":"JiFr"}],"quality_controlled":"1","publication":"Root Development. Methods and Protocols","volume":1761,"status":"public","intvolume":"      1761","publisher":"Springer","publication_status":"published","month":"03","abstract":[{"text":"Immunolocalization is a valuable tool for cell biology research that allows to rapidly determine the localization and expression levels of endogenous proteins. In plants, whole-mount in situ immunolocalization remains a challenging method, especially in tissues protected by waxy layers and complex cell wall carbohydrates. Here, we present a robust method for whole-mount in situ immunolocalization in primary root meristems and lateral root primordia in Arabidopsis thaliana. For good epitope preservation, fixation is done in an alkaline paraformaldehyde/glutaraldehyde mixture. This fixative is suitable for detecting a wide range of proteins, including integral transmembrane proteins and proteins peripherally attached to the plasma membrane. From initiation until emergence from the primary root, lateral root primordia are surrounded by several layers of differentiated tissues with a complex cell wall composition that interferes with the efficient penetration of all buffers. Therefore, immunolocalization in early lateral root primordia requires a modified method, including a strong solvent treatment for removal of hydrophobic barriers and a specific cocktail of cell wall-degrading enzymes. The presented method allows for easy, reliable, and high-quality in situ detection of the subcellular localization of endogenous proteins in primary and lateral root meristems without the need of time-consuming crosses or making translational fusions to fluorescent proteins.","lang":"eng"}],"_id":"411","date_created":"2018-12-11T11:46:20Z","series_title":"MIMB","date_published":"2018-03-11T00:00:00Z","page":"131 - 143"},{"publication_identifier":{"eissn":["1532-298X"],"issn":["1040-4651"]},"scopus_import":"1","external_id":{"isi":["000429441400018"],"pmid":["29511054"]},"date_updated":"2025-05-07T11:12:27Z","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","has_accepted_license":"1","oa_version":"Published Version","year":"2018","article_type":"original","publist_id":"7417","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"volume":30,"file_date_updated":"2022-05-23T09:12:38Z","oa":1,"publication_status":"published","license":"https://creativecommons.org/licenses/by/4.0/","_id":"412","date_published":"2018-04-09T00:00:00Z","abstract":[{"lang":"eng","text":"Clathrin-mediated endocytosis (CME) is a cellular trafficking process in which cargoes and lipids are internalized from the plasma membrane into vesicles coated with clathrin and adaptor proteins. CME is essential for many developmental and physiological processes in plants, but its underlying mechanism is not well characterised compared to that in yeast and animal systems. Here, we searched for new factors involved in CME in Arabidopsis thaliana by performing Tandem Affinity Purification of proteins that interact with clathrin light chain, a principal component of the clathrin coat. Among the confirmed interactors, we found two putative homologues of the clathrin-coat uncoating factor auxilin previously described in non-plant systems. Overexpression of AUXILIN-LIKE1 and AUXILIN-LIKE2 in A. thaliana caused an arrest of seedling growth and development. This was concomitant with inhibited endocytosis due to blocking of clathrin recruitment after the initial step of adaptor protein binding to the plasma membrane. By contrast, auxilin-like(1/2) loss-of-function lines did not present endocytosis-related developmental or cellular phenotypes under normal growth conditions. This work contributes to the on-going characterization of the endocytotic machinery in plants and provides a robust tool for conditionally and specifically interfering with CME in A. thaliana."}],"file":[{"date_created":"2022-05-23T09:12:38Z","success":1,"relation":"main_file","file_id":"11406","content_type":"application/pdf","checksum":"4e165e653b67d3f0684697f21aace5a1","access_level":"open_access","date_updated":"2022-05-23T09:12:38Z","creator":"dernst","file_size":4407538,"file_name":"2018_PlantCell_Adamowski.pdf"}],"article_processing_charge":"No","issue":"3","ddc":["580"],"doi":"10.1105/tpc.17.00785","language":[{"iso":"eng"}],"project":[{"name":"Polarity and subcellular dynamics in plants","grant_number":"282300","_id":"25716A02-B435-11E9-9278-68D0E5697425","call_identifier":"FP7"}],"pmid":1,"related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"6269"}]},"acknowledgement":"We thank James Matthew Watson, Monika Borowska, and Peggy Stolt-Bergner at ProTech Facility of the Vienna Biocenter Core Facilities for the CRISPR/CAS9 construct; Anna Müller for assistance with molecular cloning; Sebastian Bednarek, Liwen Jiang, and Daniël Van Damme for sharing published material; Matyáš Fendrych, Daniël Van Damme, and Lindy Abas for valuable discussions; and Martine De Cock for help with correcting the manuscript. This work was supported by the European Research Council under the European Union Seventh Framework Programme (FP7/2007-2013)/ERC Grant 282300 and by the Ministry of Education of the Czech Republic/MŠMT project NPUI-LO1417.","author":[{"id":"45F536D2-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6463-5257","first_name":"Maciek","full_name":"Adamowski, Maciek","last_name":"Adamowski"},{"full_name":"Narasimhan, Madhumitha","first_name":"Madhumitha","last_name":"Narasimhan","orcid":"0000-0002-8600-0671","id":"44BF24D0-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Urszula","full_name":"Kania, Urszula","last_name":"Kania","id":"4AE5C486-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Matous","full_name":"Glanc, Matous","last_name":"Glanc","orcid":"0000-0003-0619-7783","id":"1AE1EA24-02D0-11E9-9BAA-DAF4881429F2"},{"last_name":"De Jaeger","first_name":"Geert","full_name":"De Jaeger, Geert"},{"full_name":"Friml, Jirí","first_name":"Jirí","last_name":"Friml","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"type":"journal_article","day":"09","title":"A functional study of AUXILIN LIKE1 and 2 two putative clathrin uncoating factors in Arabidopsis","citation":{"mla":"Adamowski, Maciek, et al. “A Functional Study of AUXILIN LIKE1 and 2 Two Putative Clathrin Uncoating Factors in Arabidopsis.” <i>The Plant Cell</i>, vol. 30, no. 3, American Society of Plant Biologists, 2018, pp. 700–16, doi:<a href=\"https://doi.org/10.1105/tpc.17.00785\">10.1105/tpc.17.00785</a>.","chicago":"Adamowski, Maciek, Madhumitha Narasimhan, Urszula Kania, Matous Glanc, Geert De Jaeger, and Jiří Friml. “A Functional Study of AUXILIN LIKE1 and 2 Two Putative Clathrin Uncoating Factors in Arabidopsis.” <i>The Plant Cell</i>. American Society of Plant Biologists, 2018. <a href=\"https://doi.org/10.1105/tpc.17.00785\">https://doi.org/10.1105/tpc.17.00785</a>.","ista":"Adamowski M, Narasimhan M, Kania U, Glanc M, De Jaeger G, Friml J. 2018. A functional study of AUXILIN LIKE1 and 2 two putative clathrin uncoating factors in Arabidopsis. The Plant Cell. 30(3), 700–716.","ieee":"M. Adamowski, M. Narasimhan, U. Kania, M. Glanc, G. De Jaeger, and J. Friml, “A functional study of AUXILIN LIKE1 and 2 two putative clathrin uncoating factors in Arabidopsis,” <i>The Plant Cell</i>, vol. 30, no. 3. American Society of Plant Biologists, pp. 700–716, 2018.","ama":"Adamowski M, Narasimhan M, Kania U, Glanc M, De Jaeger G, Friml J. A functional study of AUXILIN LIKE1 and 2 two putative clathrin uncoating factors in Arabidopsis. <i>The Plant Cell</i>. 2018;30(3):700-716. doi:<a href=\"https://doi.org/10.1105/tpc.17.00785\">10.1105/tpc.17.00785</a>","apa":"Adamowski, M., Narasimhan, M., Kania, U., Glanc, M., De Jaeger, G., &#38; Friml, J. (2018). A functional study of AUXILIN LIKE1 and 2 two putative clathrin uncoating factors in Arabidopsis. <i>The Plant Cell</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1105/tpc.17.00785\">https://doi.org/10.1105/tpc.17.00785</a>","short":"M. Adamowski, M. Narasimhan, U. Kania, M. Glanc, G. De Jaeger, J. Friml, The Plant Cell 30 (2018) 700–716."},"ec_funded":1,"intvolume":"        30","status":"public","publication":"The Plant Cell","quality_controlled":"1","department":[{"_id":"JiFr"}],"isi":1,"publisher":"American Society of Plant Biologists","date_created":"2018-12-11T11:46:20Z","month":"04","page":"700 - 716"},{"file_date_updated":"2020-07-14T12:46:26Z","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","image":"/images/cc_by_nc_nd.png","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)"},"volume":115,"license":"https://creativecommons.org/licenses/by-nc-nd/4.0/","publication_status":"published","oa":1,"file":[{"creator":"dernst","file_size":1924101,"file_name":"2018_PNAS_Salanenka.pdf","access_level":"open_access","date_updated":"2020-07-14T12:46:26Z","checksum":"1fcf7223fb8f99559cfa80bd6f24ce44","file_id":"5700","relation":"main_file","content_type":"application/pdf","date_created":"2018-12-17T12:30:14Z"}],"date_published":"2018-04-03T00:00:00Z","_id":"428","abstract":[{"lang":"eng","text":"The plant hormone gibberellic acid (GA) is a crucial regulator of growth and development. The main paradigm of GA signaling puts forward transcriptional regulation via the degradation of DELLA transcriptional repressors. GA has also been shown to regulate tropic responses by modulation of the plasma membrane incidence of PIN auxin transporters by an unclear mechanism. Here we uncovered the cellular and molecular mechanisms by which GA redirects protein trafficking and thus regulates cell surface functionality. Photoconvertible reporters revealed that GA balances the protein traffic between the vacuole degradation route and recycling back to the cell surface. Low GA levels promote vacuolar delivery and degradation of multiple cargos, including PIN proteins, whereas high GA levels promote their recycling to the plasma membrane. This GA effect requires components of the retromer complex, such as Sorting Nexin 1 (SNX1) and its interacting, microtubule (MT)-associated protein, the Cytoplasmic Linker-Associated Protein (CLASP1). Accordingly, GA regulates the subcellular distribution of SNX1 and CLASP1, and the intact MT cytoskeleton is essential for the GA effect on trafficking. This GA cellular action occurs through DELLA proteins that regulate the MT and retromer presumably via their interaction partners Prefoldins (PFDs). Our study identified a branching of the GA signaling pathway at the level of DELLA proteins, which, in parallel to regulating transcription, also target by a nontranscriptional mechanism the retromer complex acting at the intersection of the degradation and recycling trafficking routes. By this mechanism, GA can redirect receptors and transporters to the cell surface, thus coregulating multiple processes, including PIN-dependent auxin fluxes during tropic responses."}],"article_processing_charge":"No","issue":"14","scopus_import":"1","external_id":{"isi":["000429012500073"]},"date_updated":"2025-05-07T11:12:27Z","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","publist_id":"7395","oa_version":"Published Version","has_accepted_license":"1","year":"2018","publication":"PNAS","department":[{"_id":"JiFr"}],"quality_controlled":"1","intvolume":"       115","status":"public","publisher":"National Academy of Sciences","isi":1,"month":"04","date_created":"2018-12-11T11:46:25Z","page":" 3716 - 3721","language":[{"iso":"eng"}],"ddc":["580"],"doi":"10.1073/pnas.1721760115","project":[{"name":"Polarity and subcellular dynamics in plants","grant_number":"282300","call_identifier":"FP7","_id":"25716A02-B435-11E9-9278-68D0E5697425"}],"acknowledgement":"We gratefully acknowledge M. Blázquez (Instituto de Biología Molecular y Celular de Plantas), M. Fendrych, C. Cuesta Moliner (Institute of Science and Technology Austria), M. Vanstraelen, M. Nowack (Center for Plant Systems Biology, Ghent), C. Luschnig (Universitat fur Bodenkultur Wien, Vienna), S. Simon (Central European Institute of Technology, Brno), C. Sommerville (Carnegie Institution for Science), and Y. Gu (Penn State University) for making available the materials used in this study;\r\n...funding from the European Research Council (ERC) under the European Union’s Seventh Framework Programme (FP7/2007-2013)/ERC Grant Agreement 282300.\r\nCC BY NC ND","day":"03","author":[{"id":"46DAAE7E-F248-11E8-B48F-1D18A9856A87","full_name":"Salanenka, Yuliya","first_name":"Yuliya","last_name":"Salanenka"},{"full_name":"Verstraeten, Inge","first_name":"Inge","last_name":"Verstraeten","id":"362BF7FE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-7241-2328"},{"last_name":"Löfke","full_name":"Löfke, Christian","first_name":"Christian"},{"last_name":"Tabata","first_name":"Kaori","full_name":"Tabata, Kaori","id":"7DAAEDA4-02D0-11E9-B11A-A5A4D7DFFFD0"},{"full_name":"Naramoto, Satoshi","first_name":"Satoshi","last_name":"Naramoto"},{"id":"1AE1EA24-02D0-11E9-9BAA-DAF4881429F2","orcid":"0000-0003-0619-7783","last_name":"Glanc","full_name":"Glanc, Matous","first_name":"Matous"},{"full_name":"Friml, Jirí","first_name":"Jirí","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596"}],"type":"journal_article","citation":{"short":"Y. Salanenka, I. Verstraeten, C. Löfke, K. Tabata, S. Naramoto, M. Glanc, J. Friml, PNAS 115 (2018) 3716–3721.","ama":"Salanenka Y, Verstraeten I, Löfke C, et al. Gibberellin DELLA signaling targets the retromer complex to redirect protein trafficking to the plasma membrane. <i>PNAS</i>. 2018;115(14):3716-3721. doi:<a href=\"https://doi.org/10.1073/pnas.1721760115\">10.1073/pnas.1721760115</a>","apa":"Salanenka, Y., Verstraeten, I., Löfke, C., Tabata, K., Naramoto, S., Glanc, M., &#38; Friml, J. (2018). Gibberellin DELLA signaling targets the retromer complex to redirect protein trafficking to the plasma membrane. <i>PNAS</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.1721760115\">https://doi.org/10.1073/pnas.1721760115</a>","chicago":"Salanenka, Yuliya, Inge Verstraeten, Christian Löfke, Kaori Tabata, Satoshi Naramoto, Matous Glanc, and Jiří Friml. “Gibberellin DELLA Signaling Targets the Retromer Complex to Redirect Protein Trafficking to the Plasma Membrane.” <i>PNAS</i>. National Academy of Sciences, 2018. <a href=\"https://doi.org/10.1073/pnas.1721760115\">https://doi.org/10.1073/pnas.1721760115</a>.","ieee":"Y. Salanenka <i>et al.</i>, “Gibberellin DELLA signaling targets the retromer complex to redirect protein trafficking to the plasma membrane,” <i>PNAS</i>, vol. 115, no. 14. National Academy of Sciences, pp. 3716–3721, 2018.","ista":"Salanenka Y, Verstraeten I, Löfke C, Tabata K, Naramoto S, Glanc M, Friml J. 2018. Gibberellin DELLA signaling targets the retromer complex to redirect protein trafficking to the plasma membrane. PNAS. 115(14), 3716–3721.","mla":"Salanenka, Yuliya, et al. “Gibberellin DELLA Signaling Targets the Retromer Complex to Redirect Protein Trafficking to the Plasma Membrane.” <i>PNAS</i>, vol. 115, no. 14, National Academy of Sciences, 2018, pp. 3716–21, doi:<a href=\"https://doi.org/10.1073/pnas.1721760115\">10.1073/pnas.1721760115</a>."},"ec_funded":1,"title":"Gibberellin DELLA signaling targets the retromer complex to redirect protein trafficking to the plasma membrane"},{"related_material":{"record":[{"relation":"dissertation_contains","id":"10083","status":"public"}]},"project":[{"name":"International IST Doctoral Program","grant_number":"665385","_id":"2564DBCA-B435-11E9-9278-68D0E5697425","call_identifier":"H2020"}],"acknowledgement":"This protocol was adapted from Fendrych et al., 2016. This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie Grant Agreement No. 665385, and Austrian Science Fund (FWF) [M 2128-B21]. ","language":[{"iso":"eng"}],"doi":"10.21769/BioProtoc.2685","ddc":["576","581"],"citation":{"apa":"Li, L., Krens, G., Fendrych, M., &#38; Friml, J. (2018). Real-time analysis of auxin response, cell wall pH and elongation in Arabidopsis thaliana Hypocotyls. <i>Bio-Protocol</i>. Bio-protocol. <a href=\"https://doi.org/10.21769/BioProtoc.2685\">https://doi.org/10.21769/BioProtoc.2685</a>","ama":"Li L, Krens G, Fendrych M, Friml J. Real-time analysis of auxin response, cell wall pH and elongation in Arabidopsis thaliana Hypocotyls. <i>Bio-protocol</i>. 2018;8(1). doi:<a href=\"https://doi.org/10.21769/BioProtoc.2685\">10.21769/BioProtoc.2685</a>","short":"L. Li, G. Krens, M. Fendrych, J. Friml, Bio-Protocol 8 (2018).","mla":"Li, Lanxin, et al. “Real-Time Analysis of Auxin Response, Cell Wall PH and Elongation in Arabidopsis Thaliana Hypocotyls.” <i>Bio-Protocol</i>, vol. 8, no. 1, Bio-protocol, 2018, doi:<a href=\"https://doi.org/10.21769/BioProtoc.2685\">10.21769/BioProtoc.2685</a>.","ieee":"L. Li, G. Krens, M. Fendrych, and J. Friml, “Real-time analysis of auxin response, cell wall pH and elongation in Arabidopsis thaliana Hypocotyls,” <i>Bio-protocol</i>, vol. 8, no. 1. Bio-protocol, 2018.","ista":"Li L, Krens G, Fendrych M, Friml J. 2018. Real-time analysis of auxin response, cell wall pH and elongation in Arabidopsis thaliana Hypocotyls. Bio-protocol. 8(1).","chicago":"Li, Lanxin, Gabriel Krens, Matyas Fendrych, and Jiří Friml. “Real-Time Analysis of Auxin Response, Cell Wall PH and Elongation in Arabidopsis Thaliana Hypocotyls.” <i>Bio-Protocol</i>. Bio-protocol, 2018. <a href=\"https://doi.org/10.21769/BioProtoc.2685\">https://doi.org/10.21769/BioProtoc.2685</a>."},"ec_funded":1,"title":"Real-time analysis of auxin response, cell wall pH and elongation in Arabidopsis thaliana Hypocotyls","day":"05","type":"journal_article","author":[{"last_name":"Li","first_name":"Lanxin","full_name":"Li, Lanxin","id":"367EF8FA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-5607-272X"},{"full_name":"Krens, Gabriel","first_name":"Gabriel","last_name":"Krens","id":"2B819732-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4761-5996"},{"full_name":"Fendrych, Matyas","first_name":"Matyas","last_name":"Fendrych","orcid":"0000-0002-9767-8699","id":"43905548-F248-11E8-B48F-1D18A9856A87"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","last_name":"Friml","first_name":"Jirí","full_name":"Friml, Jirí"}],"publisher":"Bio-protocol","publication":"Bio-protocol","quality_controlled":"1","department":[{"_id":"JiFr"},{"_id":"Bio"}],"intvolume":"         8","status":"public","month":"01","date_created":"2018-12-11T11:46:30Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2024-10-29T10:22:43Z","publication_identifier":{"eissn":["2331-8325"]},"article_type":"original","publist_id":"7381","oa_version":"Published Version","year":"2018","has_accepted_license":"1","publication_status":"published","oa":1,"file_date_updated":"2020-07-14T12:46:29Z","pubrep_id":"970","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"volume":8,"article_processing_charge":"No","issue":"1","file":[{"file_name":"IST-2018-970-v1+1_2018_Lanxin_Real-time_analysis.pdf","file_size":11352389,"creator":"system","checksum":"6644ba698206eda32b0abf09128e63e3","date_updated":"2020-07-14T12:46:29Z","access_level":"open_access","content_type":"application/pdf","file_id":"5299","relation":"main_file","date_created":"2018-12-12T10:17:43Z"}],"_id":"442","date_published":"2018-01-05T00:00:00Z","abstract":[{"lang":"eng","text":"The rapid auxin-triggered growth of the Arabidopsis hypocotyls involves the nuclear TIR1/AFB-Aux/IAA signaling and is accompanied by acidification of the apoplast and cell walls (Fendrych et al., 2016). Here, we describe in detail the method for analysis of the elongation and the TIR1/AFB-Aux/IAA-dependent auxin response in hypocotyl segments as well as the determination of relative values of the cell wall pH."}]},{"project":[{"name":"Polarity and subcellular dynamics in plants","grant_number":"282300","call_identifier":"FP7","_id":"25716A02-B435-11E9-9278-68D0E5697425"}],"related_material":{"record":[{"id":"1127","relation":"dissertation_contains","status":"public"},{"id":"7172","relation":"dissertation_contains","status":"public"},{"id":"8822","relation":"dissertation_contains","status":"public"}]},"language":[{"iso":"eng"}],"doi":"10.1371/journal.pgen.1007177","ddc":["581"],"ec_funded":1,"citation":{"mla":"Prat, Tomas, et al. “WRKY23 Is a Component of the Transcriptional Network Mediating Auxin Feedback on PIN Polarity.” <i>PLoS Genetics</i>, vol. 14, no. 1, Public Library of Science, 2018, doi:<a href=\"https://doi.org/10.1371/journal.pgen.1007177\">10.1371/journal.pgen.1007177</a>.","chicago":"Prat, Tomas, Jakub Hajny, Wim Grunewald, Mina K Vasileva, Gergely Molnar, Ricardo Tejos, Markus Schmid, Michael Sauer, and Jiří Friml. “WRKY23 Is a Component of the Transcriptional Network Mediating Auxin Feedback on PIN Polarity.” <i>PLoS Genetics</i>. Public Library of Science, 2018. <a href=\"https://doi.org/10.1371/journal.pgen.1007177\">https://doi.org/10.1371/journal.pgen.1007177</a>.","ista":"Prat T, Hajny J, Grunewald W, Vasileva MK, Molnar G, Tejos R, Schmid M, Sauer M, Friml J. 2018. WRKY23 is a component of the transcriptional network mediating auxin feedback on PIN polarity. PLoS Genetics. 14(1).","ieee":"T. Prat <i>et al.</i>, “WRKY23 is a component of the transcriptional network mediating auxin feedback on PIN polarity,” <i>PLoS Genetics</i>, vol. 14, no. 1. Public Library of Science, 2018.","ama":"Prat T, Hajny J, Grunewald W, et al. WRKY23 is a component of the transcriptional network mediating auxin feedback on PIN polarity. <i>PLoS Genetics</i>. 2018;14(1). doi:<a href=\"https://doi.org/10.1371/journal.pgen.1007177\">10.1371/journal.pgen.1007177</a>","apa":"Prat, T., Hajny, J., Grunewald, W., Vasileva, M. K., Molnar, G., Tejos, R., … Friml, J. (2018). WRKY23 is a component of the transcriptional network mediating auxin feedback on PIN polarity. <i>PLoS Genetics</i>. Public Library of Science. <a href=\"https://doi.org/10.1371/journal.pgen.1007177\">https://doi.org/10.1371/journal.pgen.1007177</a>","short":"T. Prat, J. Hajny, W. Grunewald, M.K. Vasileva, G. Molnar, R. Tejos, M. Schmid, M. Sauer, J. Friml, PLoS Genetics 14 (2018)."},"title":"WRKY23 is a component of the transcriptional network mediating auxin feedback on PIN polarity","day":"29","author":[{"last_name":"Prat","full_name":"Prat, Tomas","first_name":"Tomas","id":"3DA3BFEE-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Hajny","full_name":"Hajny, Jakub","first_name":"Jakub","id":"4800CC20-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2140-7195"},{"last_name":"Grunewald","full_name":"Grunewald, Wim","first_name":"Wim"},{"id":"3407EB18-F248-11E8-B48F-1D18A9856A87","last_name":"Vasileva","full_name":"Vasileva, Mina K","first_name":"Mina K"},{"id":"34F1AF46-F248-11E8-B48F-1D18A9856A87","last_name":"Molnar","first_name":"Gergely","full_name":"Molnar, Gergely"},{"last_name":"Tejos","first_name":"Ricardo","full_name":"Tejos, Ricardo"},{"first_name":"Markus","full_name":"Schmid, Markus","last_name":"Schmid"},{"first_name":"Michael","full_name":"Sauer, Michael","last_name":"Sauer"},{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí","first_name":"Jirí","last_name":"Friml"}],"type":"journal_article","publisher":"Public Library of Science","isi":1,"quality_controlled":"1","department":[{"_id":"JiFr"}],"publication":"PLoS Genetics","status":"public","intvolume":"        14","month":"01","date_created":"2018-12-11T11:46:32Z","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","date_updated":"2025-05-07T11:12:28Z","scopus_import":"1","external_id":{"isi":["000423718600034"]},"publist_id":"7373","has_accepted_license":"1","year":"2018","oa_version":"Published Version","publication_status":"published","oa":1,"file_date_updated":"2020-07-14T12:46:30Z","volume":14,"pubrep_id":"967","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"issue":"1","article_processing_charge":"Yes","file":[{"date_created":"2018-12-12T10:10:52Z","content_type":"application/pdf","file_id":"4843","relation":"main_file","date_updated":"2020-07-14T12:46:30Z","access_level":"open_access","checksum":"0276d66788ec076f4924164a39e6a712","file_name":"IST-2018-967-v1+1_journal.pgen.1007177.pdf","creator":"system","file_size":24709062}],"date_published":"2018-01-29T00:00:00Z","_id":"449","abstract":[{"lang":"eng","text":"Auxin is unique among plant hormones due to its directional transport that is mediated by the polarly distributed PIN auxin transporters at the plasma membrane. The canalization hypothesis proposes that the auxin feedback on its polar flow is a crucial, plant-specific mechanism mediating multiple self-organizing developmental processes. Here, we used the auxin effect on the PIN polar localization in Arabidopsis thaliana roots as a proxy for the auxin feedback on the PIN polarity during canalization. We performed microarray experiments to find regulators of this process that act downstream of auxin. We identified genes that were transcriptionally regulated by auxin in an AXR3/IAA17- and ARF7/ARF19-dependent manner. Besides the known components of the PIN polarity, such as PID and PIP5K kinases, a number of potential new regulators were detected, among which the WRKY23 transcription factor, which was characterized in more detail. Gain- and loss-of-function mutants confirmed a role for WRKY23 in mediating the auxin effect on the PIN polarity. Accordingly, processes requiring auxin-mediated PIN polarity rearrangements, such as vascular tissue development during leaf venation, showed a higher WRKY23 expression and required the WRKY23 activity. Our results provide initial insights into the auxin transcriptional network acting upstream of PIN polarization and, potentially, canalization-mediated plant development."}]},{"title":"Light sheet fluorescence microscopy of plant roots growing on the surface of a gel","citation":{"mla":"von Wangenheim, Daniel, et al. “Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel.” <i>Journal of Visualized Experiments JoVE</i>, vol. 2017, no. 119, e55044, Journal of Visualized Experiments, 2017, doi:<a href=\"https://doi.org/10.3791/55044\">10.3791/55044</a>.","chicago":"Wangenheim, Daniel von, Robert Hauschild, and Jiří Friml. “Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel.” <i>Journal of Visualized Experiments JoVE</i>. Journal of Visualized Experiments, 2017. <a href=\"https://doi.org/10.3791/55044\">https://doi.org/10.3791/55044</a>.","ieee":"D. von Wangenheim, R. Hauschild, and J. Friml, “Light sheet fluorescence microscopy of plant roots growing on the surface of a gel,” <i>Journal of visualized experiments JoVE</i>, vol. 2017, no. 119. Journal of Visualized Experiments, 2017.","ista":"von Wangenheim D, Hauschild R, Friml J. 2017. Light sheet fluorescence microscopy of plant roots growing on the surface of a gel. Journal of visualized experiments JoVE. 2017(119), e55044.","ama":"von Wangenheim D, Hauschild R, Friml J. Light sheet fluorescence microscopy of plant roots growing on the surface of a gel. <i>Journal of visualized experiments JoVE</i>. 2017;2017(119). doi:<a href=\"https://doi.org/10.3791/55044\">10.3791/55044</a>","apa":"von Wangenheim, D., Hauschild, R., &#38; Friml, J. (2017). Light sheet fluorescence microscopy of plant roots growing on the surface of a gel. <i>Journal of Visualized Experiments JoVE</i>. Journal of Visualized Experiments. <a href=\"https://doi.org/10.3791/55044\">https://doi.org/10.3791/55044</a>","short":"D. von Wangenheim, R. Hauschild, J. Friml, Journal of Visualized Experiments JoVE 2017 (2017)."},"ec_funded":1,"type":"journal_article","author":[{"full_name":"Von Wangenheim, Daniel","first_name":"Daniel","last_name":"Von Wangenheim","orcid":"0000-0002-6862-1247","id":"49E91952-F248-11E8-B48F-1D18A9856A87"},{"id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9843-3522","first_name":"Robert","full_name":"Hauschild, Robert","last_name":"Hauschild"},{"first_name":"Jirí","full_name":"Friml, Jirí","last_name":"Friml","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"day":"18","project":[{"call_identifier":"FP7","_id":"25681D80-B435-11E9-9278-68D0E5697425","grant_number":"291734","name":"International IST Postdoc Fellowship Programme"},{"name":"Polarity and subcellular dynamics in plants","grant_number":"282300","_id":"25716A02-B435-11E9-9278-68D0E5697425","call_identifier":"FP7"}],"related_material":{"record":[{"status":"public","relation":"popular_science","id":"5565"}]},"ddc":["580"],"doi":"10.3791/55044","language":[{"iso":"eng"}],"date_created":"2018-12-11T11:50:01Z","month":"01","isi":1,"publisher":"Journal of Visualized Experiments","status":"public","intvolume":"      2017","publication":"Journal of visualized experiments JoVE","department":[{"_id":"JiFr"},{"_id":"Bio"}],"year":"2017","has_accepted_license":"1","oa_version":"Published Version","publist_id":"6302","acknowledged_ssus":[{"_id":"M-Shop"},{"_id":"Bio"}],"external_id":{"isi":["000397847200041"]},"scopus_import":"1","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","date_updated":"2025-05-07T11:12:33Z","article_processing_charge":"No","issue":"119","abstract":[{"text":"One of the key questions in understanding plant development is how single cells behave in a larger context of the tissue. Therefore, it requires the observation of the whole organ with a high spatial- as well as temporal resolution over prolonged periods of time, which may cause photo-toxic effects. This protocol shows a plant sample preparation method for light-sheet microscopy, which is characterized by mounting the plant vertically on the surface of a gel. The plant is mounted in such a way that the roots are submerged in a liquid medium while the leaves remain in the air. In order to ensure photosynthetic activity of the plant, a custom-made lighting system illuminates the leaves. To keep the roots in darkness the water surface is covered with sheets of black plastic foil. This method allows long-term imaging of plant organ development in standardized conditions. ","lang":"eng"}],"_id":"1078","date_published":"2017-01-18T00:00:00Z","file":[{"date_created":"2018-12-12T10:16:31Z","relation":"main_file","file_id":"5219","content_type":"application/pdf","access_level":"open_access","date_updated":"2018-12-12T10:16:31Z","file_size":57678,"creator":"system","file_name":"IST-2017-808-v1+1_2017_VWangenheim_list.pdf"},{"file_name":"IST-2017-808-v1+2_2017_VWangenheim_article.pdf","file_size":1317820,"creator":"system","date_updated":"2018-12-12T10:16:32Z","access_level":"open_access","content_type":"application/pdf","file_id":"5220","relation":"main_file","date_created":"2018-12-12T10:16:32Z"}],"article_number":"e55044","oa":1,"publication_status":"published","pubrep_id":"808","volume":2017,"file_date_updated":"2018-12-12T10:16:32Z"},{"oa":1,"publication_status":"published","volume":7,"pubrep_id":"803","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"file_date_updated":"2018-12-12T10:18:09Z","article_processing_charge":"No","abstract":[{"text":"The phytohormone auxin is a major determinant and regulatory component important for plant development. Auxin transport between cells is mediated by a complex system of transporters such as AUX1/LAX, PIN, and ABCB proteins, and their localization and activity is thought to be influenced by phosphatases and kinases. Flavonols have been shown to alter auxin transport activity and changes in flavonol accumulation in the Arabidopsis thaliana rol1-2 mutant cause defects in auxin transport and seedling development. A new mutation in ROOTS CURL IN NPA 1 (RCN1), encoding a regulatory subunit of the phosphatase PP2A, was found to suppress the growth defects of rol1-2 without changing the flavonol content. rol1-2 rcn1-3 double mutants show wild type-like auxin transport activity while levels of free auxin are not affected by rcn1-3. In the rol1-2 mutant, PIN2 shows a flavonol-induced basal-to-apical shift in polar localization which is reversed in the rol1-2 rcn1-3 to basal localization. In vivo analysis of PINOID action, a kinase known to influence PIN protein localization in a PP2A-antagonistic manner, revealed a negative impact of flavonols on PINOID activity. Together, these data suggest that flavonols affect auxin transport by modifying the antagonistic kinase/phosphatase equilibrium.","lang":"eng"}],"_id":"1110","date_published":"2017-02-06T00:00:00Z","article_number":"41906","file":[{"file_name":"IST-2017-803-v1+1_srep41906.pdf","file_size":1654496,"creator":"system","date_updated":"2018-12-12T10:18:09Z","access_level":"open_access","content_type":"application/pdf","file_id":"5328","relation":"main_file","date_created":"2018-12-12T10:18:09Z"}],"publication_identifier":{"issn":["20452322"]},"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","date_updated":"2025-05-07T11:12:29Z","external_id":{"isi":["000393367600001"]},"scopus_import":"1","has_accepted_license":"1","oa_version":"Published Version","year":"2017","publist_id":"6258","isi":1,"publisher":"Nature Publishing Group","intvolume":"         7","status":"public","quality_controlled":"1","department":[{"_id":"JiFr"}],"publication":"Scientific Reports","date_created":"2018-12-11T11:50:12Z","month":"02","acknowledgement":"European Research Council (project ERC-2011-StG-20101109-PSDP), European Social Fund (CZ.1.07/2.3.00/20.0043) and the Czech Science Foundation (GA13-40637S) [JF].","project":[{"name":"Polarity and subcellular dynamics in plants","grant_number":"282300","call_identifier":"FP7","_id":"25716A02-B435-11E9-9278-68D0E5697425"}],"doi":"10.1038/srep41906","ddc":["581"],"language":[{"iso":"eng"}],"title":"Flavonol-induced changes in PIN2 polarity and auxin transport in the Arabidopsis thaliana rol1-2 mutant require phosphatase activity","ec_funded":1,"citation":{"mla":"Kuhn, Benjamin, et al. “Flavonol-Induced Changes in PIN2 Polarity and Auxin Transport in the Arabidopsis Thaliana Rol1-2 Mutant Require Phosphatase Activity.” <i>Scientific Reports</i>, vol. 7, 41906, Nature Publishing Group, 2017, doi:<a href=\"https://doi.org/10.1038/srep41906\">10.1038/srep41906</a>.","chicago":"Kuhn, Benjamin, Tomasz Nodzyński, Sanae Errafi, Rahel Bucher, Shibu Gupta, Bibek Aryal, Petre Dobrev, et al. “Flavonol-Induced Changes in PIN2 Polarity and Auxin Transport in the Arabidopsis Thaliana Rol1-2 Mutant Require Phosphatase Activity.” <i>Scientific Reports</i>. Nature Publishing Group, 2017. <a href=\"https://doi.org/10.1038/srep41906\">https://doi.org/10.1038/srep41906</a>.","ieee":"B. Kuhn <i>et al.</i>, “Flavonol-induced changes in PIN2 polarity and auxin transport in the Arabidopsis thaliana rol1-2 mutant require phosphatase activity,” <i>Scientific Reports</i>, vol. 7. Nature Publishing Group, 2017.","ista":"Kuhn B, Nodzyński T, Errafi S, Bucher R, Gupta S, Aryal B, Dobrev P, Bigler L, Geisler M, Zažímalová E, Friml J, Ringli C. 2017. Flavonol-induced changes in PIN2 polarity and auxin transport in the Arabidopsis thaliana rol1-2 mutant require phosphatase activity. Scientific Reports. 7, 41906.","ama":"Kuhn B, Nodzyński T, Errafi S, et al. Flavonol-induced changes in PIN2 polarity and auxin transport in the Arabidopsis thaliana rol1-2 mutant require phosphatase activity. <i>Scientific Reports</i>. 2017;7. doi:<a href=\"https://doi.org/10.1038/srep41906\">10.1038/srep41906</a>","apa":"Kuhn, B., Nodzyński, T., Errafi, S., Bucher, R., Gupta, S., Aryal, B., … Ringli, C. (2017). Flavonol-induced changes in PIN2 polarity and auxin transport in the Arabidopsis thaliana rol1-2 mutant require phosphatase activity. <i>Scientific Reports</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/srep41906\">https://doi.org/10.1038/srep41906</a>","short":"B. Kuhn, T. Nodzyński, S. Errafi, R. Bucher, S. Gupta, B. Aryal, P. Dobrev, L. Bigler, M. Geisler, E. Zažímalová, J. Friml, C. Ringli, Scientific Reports 7 (2017)."},"author":[{"full_name":"Kuhn, Benjamin","first_name":"Benjamin","last_name":"Kuhn"},{"last_name":"Nodzyński","full_name":"Nodzyński, Tomasz","first_name":"Tomasz"},{"first_name":"Sanae","full_name":"Errafi, Sanae","last_name":"Errafi"},{"first_name":"Rahel","full_name":"Bucher, Rahel","last_name":"Bucher"},{"last_name":"Gupta","full_name":"Gupta, Shibu","first_name":"Shibu"},{"last_name":"Aryal","first_name":"Bibek","full_name":"Aryal, Bibek"},{"last_name":"Dobrev","full_name":"Dobrev, Petre","first_name":"Petre"},{"last_name":"Bigler","full_name":"Bigler, Laurent","first_name":"Laurent"},{"last_name":"Geisler","full_name":"Geisler, Markus","first_name":"Markus"},{"full_name":"Zažímalová, Eva","first_name":"Eva","last_name":"Zažímalová"},{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jirí","first_name":"Jirí"},{"last_name":"Ringli","full_name":"Ringli, Christoph","first_name":"Christoph"}],"type":"journal_article","day":"06"},{"article_processing_charge":"No","file":[{"date_updated":"2019-04-05T08:45:14Z","access_level":"closed","checksum":"d192c7c6c5ea32c8432437286dc4909e","file_name":"IST_Austria_Thesis_Tomáš_Prát.pdf","creator":"dernst","file_size":10285946,"date_created":"2019-04-05T08:45:14Z","content_type":"application/pdf","relation":"main_file","file_id":"6209"},{"file_size":9802991,"creator":"dernst","file_name":"2017_Thesis_Prat.pdf","access_level":"open_access","date_updated":"2021-02-22T11:52:56Z","checksum":"bab18b52cf98145926042d8ed99fdb3b","file_id":"9185","relation":"main_file","content_type":"application/pdf","success":1,"date_created":"2021-02-22T11:52:56Z"}],"_id":"1127","date_published":"2017-01-12T00:00:00Z","abstract":[{"lang":"eng","text":"Plant hormone auxin and its transport between cells belong to the most important\r\nmechanisms controlling plant development. Auxin itself could change localization of PINs and\r\nthereby control direction of its own flow. We performed an expression profiling experiment\r\nin Arabidopsis roots to identify potential regulators of PIN polarity which are transcriptionally\r\nregulated by auxin signalling. We identified several novel regulators and performed a detailed\r\ncharacterization of the transcription factor WRKY23 (At2g47260) and its role in auxin\r\nfeedback on PIN polarity. Gain-of-function and dominant-negative mutants revealed that\r\nWRKY23 plays a crucial role in mediating the auxin effect on PIN polarity. In concordance,\r\ntypical polar auxin transport processes such as gravitropism and leaf vascular pattern\r\nformation were disturbed by interfering with WRKY23 function.\r\nIn order to identify direct targets of WRKY23, we performed consequential expression\r\nprofiling experiments using a WRKY23 inducible gain-of-function line and dominant-negative\r\nWRKY23 line that is defunct in PIN re-arrangement. Among several genes mostly related to\r\nthe groups of cell wall and defense process regulators, we identified LYSINE-HISTIDINE\r\nTRANSPORTER 1 (LHT1; At5g40780), a small amino acid permease gene from the amino\r\nacid/auxin permease family (AAAP), we present its detailed characterisation in auxin feedback\r\non PIN repolarization, identified its transcriptional regulation, we propose a potential\r\nmechanism of its action. Moreover, we identified also a member of receptor-like protein\r\nkinase LRR-RLK (LEUCINE-RICH REPEAT TRANSMEMBRANE PROTEIN KINASE PROTEIN 1;\r\nLRRK1; At1g05700), which also affects auxin-dependent PIN re-arrangement. We described\r\nits transcriptional behaviour, subcellular localization. Based on global expression data, we\r\ntried to identify ligand responsible for mechanism of signalling and suggest signalling partner\r\nand interactors. Additionally, we described role of novel phytohormone group, strigolactone,\r\nin auxin-dependent PIN re-arrangement, that could be a fundament for future studies in this\r\nfield.\r\nOur results provide first insights into an auxin transcriptional network targeting PIN\r\nlocalization and thus regulating plant development. We highlighted WRKY23 transcriptional\r\nnetwork and characterised its mediatory role in plant development. We identified direct\r\neffectors of this network, LHT1 and LRRK1, and describe their roles in PIN re-arrangement and\r\nPIN-dependent auxin transport processes."}],"publication_status":"published","oa":1,"file_date_updated":"2021-02-22T11:52:56Z","publist_id":"6233","oa_version":"Published Version","year":"2017","has_accepted_license":"1","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","date_updated":"2025-05-07T11:12:27Z","publication_identifier":{"issn":["2663-337X"]},"page":"131","month":"01","supervisor":[{"last_name":"Friml","first_name":"Jiří","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596"}],"date_created":"2018-12-11T11:50:17Z","publisher":"Institute of Science and Technology Austria","degree_awarded":"PhD","department":[{"_id":"JiFr"}],"status":"public","citation":{"short":"T. Prat, Identification of Novel Regulators of PIN Polarity and Development of Novel Auxin Sensor, Institute of Science and Technology Austria, 2017.","ama":"Prat T. Identification of novel regulators of PIN polarity and development of novel auxin sensor. 2017.","apa":"Prat, T. (2017). <i>Identification of novel regulators of PIN polarity and development of novel auxin sensor</i>. Institute of Science and Technology Austria.","chicago":"Prat, Tomas. “Identification of Novel Regulators of PIN Polarity and Development of Novel Auxin Sensor.” Institute of Science and Technology Austria, 2017.","ieee":"T. Prat, “Identification of novel regulators of PIN polarity and development of novel auxin sensor,” Institute of Science and Technology Austria, 2017.","ista":"Prat T. 2017. Identification of novel regulators of PIN polarity and development of novel auxin sensor. Institute of Science and Technology Austria.","mla":"Prat, Tomas. <i>Identification of Novel Regulators of PIN Polarity and Development of Novel Auxin Sensor</i>. Institute of Science and Technology Austria, 2017."},"title":"Identification of novel regulators of PIN polarity and development of novel auxin sensor","day":"12","type":"dissertation","author":[{"id":"3DA3BFEE-F248-11E8-B48F-1D18A9856A87","full_name":"Prat, Tomas","first_name":"Tomas","last_name":"Prat"}],"alternative_title":["ISTA Thesis"],"related_material":{"record":[{"id":"449","relation":"part_of_dissertation","status":"public"}]},"acknowledgement":"I would like to first acknowledge my supervisor Jiří Friml for support, kind advice and patience. It was a pleasure to be a part of your lab, Jiří. I will remember the atmosphere present in auxin lab at VIB in Ghent and at IST in Klosterneuburg forever. I would like to thank all past and present lab members for the friendship and friendly and scientific environment in the groups. It was so nice to cooperate with you, guys. There was always someone who helped me with experiments, troubleshoot issues coming from our work etc. At this place, I would like to thank especially to Gergo Molnár. I’m happy (and lucky) that I have met him; he naturally became my tutor and guide through my PhD. From no one else during my entire professional career, I’ve learned that much.","language":[{"iso":"eng"}],"ddc":["580"]},{"publication_status":"published","oa":1,"file_date_updated":"2020-07-14T12:44:36Z","volume":173,"article_processing_charge":"No","issue":"1","file":[{"file_name":"2016_PlantPhysi_Steenackers.pdf","file_size":4109142,"creator":"dernst","checksum":"fd4d1cfe7ed70e54bb12ae3881f3fb91","date_updated":"2020-07-14T12:44:36Z","access_level":"open_access","content_type":"application/pdf","file_id":"7040","relation":"main_file","date_created":"2019-11-18T16:12:25Z"}],"_id":"1159","date_published":"2017-01-01T00:00:00Z","abstract":[{"lang":"eng","text":"Auxin steers numerous physiological processes in plants, making the tight control of its endogenous levels and spatiotemporal distribution a necessity. This regulation is achieved by different mechanisms, including auxin biosynthesis, metabolic conversions, degradation, and transport. Here, we introduce cis-cinnamic acid (c-CA) as a novel and unique addition to a small group of endogenous molecules affecting in planta auxin concentrations. c-CA is the photo-isomerization product of the phenylpropanoid pathway intermediate trans-CA (t-CA). When grown on c-CA-containing medium, an evolutionary diverse set of plant species were shown to exhibit phenotypes characteristic for high auxin levels, including inhibition of primary root growth, induction of root hairs, and promotion of adventitious and lateral rooting. By molecular docking and receptor binding assays, we showed that c-CA itself is neither an auxin nor an anti-auxin, and auxin profiling data revealed that c-CA does not significantly interfere with auxin biosynthesis. Single cell-based auxin accumulation assays showed that c-CA, and not t-CA, is a potent inhibitor of auxin efflux. Auxin signaling reporters detected changes in spatiotemporal distribution of the auxin response along the root of c-CA-treated plants, and long-distance auxin transport assays showed no inhibition of rootward auxin transport. Overall, these results suggest that the phenotypes of c-CA-treated plants are the consequence of a local change in auxin accumulation, induced by the inhibition of auxin efflux. This work reveals a novel mechanism how plants may regulate auxin levels and adds a novel, naturally occurring molecule to the chemical toolbox for the studies of auxin homeostasis."}],"scopus_import":"1","external_id":{"isi":["000394135800041"],"pmid":["27837086"]},"date_updated":"2025-05-07T11:12:30Z","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","publication_identifier":{"issn":["0032-0889"]},"article_type":"original","publist_id":"6199","year":"2017","has_accepted_license":"1","oa_version":"Submitted Version","publisher":"American Society of Plant Biologists","isi":1,"publication":"Plant Physiology","department":[{"_id":"JiFr"}],"quality_controlled":"1","status":"public","intvolume":"       173","page":"552 - 565","month":"01","date_created":"2018-12-11T11:50:28Z","project":[{"_id":"25716A02-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","grant_number":"282300","name":"Polarity and subcellular dynamics in plants"}],"pmid":1,"language":[{"iso":"eng"}],"ddc":["580"],"doi":"10.1104/pp.16.00943","citation":{"mla":"Steenackers, Ward, et al. “Cis-Cinnamic Acid Is a Novel Natural Auxin Efflux Inhibitor That Promotes Lateral Root Formation.” <i>Plant Physiology</i>, vol. 173, no. 1, American Society of Plant Biologists, 2017, pp. 552–65, doi:<a href=\"https://doi.org/10.1104/pp.16.00943\">10.1104/pp.16.00943</a>.","ieee":"W. Steenackers <i>et al.</i>, “Cis-cinnamic acid is a novel natural auxin efflux inhibitor that promotes lateral root formation,” <i>Plant Physiology</i>, vol. 173, no. 1. American Society of Plant Biologists, pp. 552–565, 2017.","ista":"Steenackers W, Klíma P, Quareshy M, Cesarino I, Kumpf R, Corneillie S, Araújo P, Viaene T, Goeminne G, Nowack M, Ljung K, Friml J, Blakeslee J, Novák O, Zažímalová E, Napier R, Boerjan W, Vanholme B. 2017. Cis-cinnamic acid is a novel natural auxin efflux inhibitor that promotes lateral root formation. Plant Physiology. 173(1), 552–565.","chicago":"Steenackers, Ward, Petr Klíma, Mussa Quareshy, Igor Cesarino, Robert Kumpf, Sander Corneillie, Pedro Araújo, et al. “Cis-Cinnamic Acid Is a Novel Natural Auxin Efflux Inhibitor That Promotes Lateral Root Formation.” <i>Plant Physiology</i>. American Society of Plant Biologists, 2017. <a href=\"https://doi.org/10.1104/pp.16.00943\">https://doi.org/10.1104/pp.16.00943</a>.","apa":"Steenackers, W., Klíma, P., Quareshy, M., Cesarino, I., Kumpf, R., Corneillie, S., … Vanholme, B. (2017). Cis-cinnamic acid is a novel natural auxin efflux inhibitor that promotes lateral root formation. <i>Plant Physiology</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1104/pp.16.00943\">https://doi.org/10.1104/pp.16.00943</a>","ama":"Steenackers W, Klíma P, Quareshy M, et al. Cis-cinnamic acid is a novel natural auxin efflux inhibitor that promotes lateral root formation. <i>Plant Physiology</i>. 2017;173(1):552-565. doi:<a href=\"https://doi.org/10.1104/pp.16.00943\">10.1104/pp.16.00943</a>","short":"W. Steenackers, P. Klíma, M. Quareshy, I. Cesarino, R. Kumpf, S. Corneillie, P. Araújo, T. Viaene, G. Goeminne, M. Nowack, K. Ljung, J. Friml, J. Blakeslee, O. Novák, E. Zažímalová, R. Napier, W. Boerjan, B. Vanholme, Plant Physiology 173 (2017) 552–565."},"ec_funded":1,"title":"Cis-cinnamic acid is a novel natural auxin efflux inhibitor that promotes lateral root formation","day":"01","author":[{"first_name":"Ward","full_name":"Steenackers, Ward","last_name":"Steenackers"},{"last_name":"Klíma","first_name":"Petr","full_name":"Klíma, Petr"},{"last_name":"Quareshy","full_name":"Quareshy, Mussa","first_name":"Mussa"},{"full_name":"Cesarino, Igor","first_name":"Igor","last_name":"Cesarino"},{"first_name":"Robert","full_name":"Kumpf, Robert","last_name":"Kumpf"},{"first_name":"Sander","full_name":"Corneillie, Sander","last_name":"Corneillie"},{"last_name":"Araújo","first_name":"Pedro","full_name":"Araújo, Pedro"},{"last_name":"Viaene","first_name":"Tom","full_name":"Viaene, Tom"},{"first_name":"Geert","full_name":"Goeminne, Geert","last_name":"Goeminne"},{"last_name":"Nowack","full_name":"Nowack, Moritz","first_name":"Moritz"},{"last_name":"Ljung","first_name":"Karin","full_name":"Ljung, Karin"},{"full_name":"Friml, Jirí","first_name":"Jirí","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596"},{"last_name":"Blakeslee","full_name":"Blakeslee, Joshua","first_name":"Joshua"},{"last_name":"Novák","full_name":"Novák, Ondřej","first_name":"Ondřej"},{"last_name":"Zažímalová","first_name":"Eva","full_name":"Zažímalová, Eva"},{"full_name":"Napier, Richard","first_name":"Richard","last_name":"Napier"},{"full_name":"Boerjan, Wout","first_name":"Wout","last_name":"Boerjan"},{"last_name":"Vanholme","full_name":"Vanholme, Bartel","first_name":"Bartel"}],"type":"journal_article"},{"title":"BEN3/BIG2 ARF GEF is involved in brefeldin a-sensitive trafficking at the trans-Golgi network/early endosome in Arabidopsis thaliana","citation":{"short":"S. Kitakura, M. Adamowski, Y. Matsuura, L. Santuari, H. Kouno, K. Arima, C. Hardtke, J. Friml, T. Kakimoto, H. Tanaka, Plant and Cell Physiology 58 (2017).","apa":"Kitakura, S., Adamowski, M., Matsuura, Y., Santuari, L., Kouno, H., Arima, K., … Tanaka, H. (2017). BEN3/BIG2 ARF GEF is involved in brefeldin a-sensitive trafficking at the trans-Golgi network/early endosome in Arabidopsis thaliana. <i>Plant and Cell Physiology</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/pcp/pcx118\">https://doi.org/10.1093/pcp/pcx118</a>","ama":"Kitakura S, Adamowski M, Matsuura Y, et al. BEN3/BIG2 ARF GEF is involved in brefeldin a-sensitive trafficking at the trans-Golgi network/early endosome in Arabidopsis thaliana. <i>Plant and Cell Physiology</i>. 2017;58(10). doi:<a href=\"https://doi.org/10.1093/pcp/pcx118\">10.1093/pcp/pcx118</a>","ista":"Kitakura S, Adamowski M, Matsuura Y, Santuari L, Kouno H, Arima K, Hardtke C, Friml J, Kakimoto T, Tanaka H. 2017. BEN3/BIG2 ARF GEF is involved in brefeldin a-sensitive trafficking at the trans-Golgi network/early endosome in Arabidopsis thaliana. Plant and Cell Physiology. 58(10), 1801–1811.","ieee":"S. Kitakura <i>et al.</i>, “BEN3/BIG2 ARF GEF is involved in brefeldin a-sensitive trafficking at the trans-Golgi network/early endosome in Arabidopsis thaliana,” <i>Plant and Cell Physiology</i>, vol. 58, no. 10. Oxford University Press, 2017.","chicago":"Kitakura, Saeko, Maciek Adamowski, Yuki Matsuura, Luca Santuari, Hirotaka Kouno, Kohei Arima, Christian Hardtke, Jiří Friml, Tatsuo Kakimoto, and Hirokazu Tanaka. “BEN3/BIG2 ARF GEF Is Involved in Brefeldin a-Sensitive Trafficking at the Trans-Golgi Network/Early Endosome in Arabidopsis Thaliana.” <i>Plant and Cell Physiology</i>. Oxford University Press, 2017. <a href=\"https://doi.org/10.1093/pcp/pcx118\">https://doi.org/10.1093/pcp/pcx118</a>.","mla":"Kitakura, Saeko, et al. “BEN3/BIG2 ARF GEF Is Involved in Brefeldin a-Sensitive Trafficking at the Trans-Golgi Network/Early Endosome in Arabidopsis Thaliana.” <i>Plant and Cell Physiology</i>, vol. 58, no. 10, 1801–1811, Oxford University Press, 2017, doi:<a href=\"https://doi.org/10.1093/pcp/pcx118\">10.1093/pcp/pcx118</a>."},"type":"journal_article","author":[{"last_name":"Kitakura","full_name":"Kitakura, Saeko","first_name":"Saeko"},{"full_name":"Adamowski, Maciek","first_name":"Maciek","last_name":"Adamowski","id":"45F536D2-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6463-5257"},{"full_name":"Matsuura, Yuki","first_name":"Yuki","last_name":"Matsuura"},{"last_name":"Santuari","full_name":"Santuari, Luca","first_name":"Luca"},{"full_name":"Kouno, Hirotaka","first_name":"Hirotaka","last_name":"Kouno"},{"last_name":"Arima","first_name":"Kohei","full_name":"Arima, Kohei"},{"last_name":"Hardtke","first_name":"Christian","full_name":"Hardtke, Christian"},{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí","first_name":"Jirí","last_name":"Friml"},{"last_name":"Kakimoto","first_name":"Tatsuo","full_name":"Kakimoto, Tatsuo"},{"first_name":"Hirokazu","full_name":"Tanaka, Hirokazu","last_name":"Tanaka"}],"day":"21","pmid":1,"ddc":["581"],"doi":"10.1093/pcp/pcx118","language":[{"iso":"eng"}],"date_created":"2018-12-11T11:48:34Z","month":"08","isi":1,"publisher":"Oxford University Press","status":"public","intvolume":"        58","publication":"Plant and Cell Physiology","quality_controlled":"1","department":[{"_id":"JiFr"}],"has_accepted_license":"1","year":"2017","oa_version":"Submitted Version","publist_id":"6854","publication_identifier":{"issn":["00320781"]},"external_id":{"isi":["000413220400019"],"pmid":["29016942"]},"scopus_import":"1","date_updated":"2023-09-27T11:00:19Z","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","article_processing_charge":"No","issue":"10","_id":"799","date_published":"2017-08-21T00:00:00Z","abstract":[{"lang":"eng","text":"Membrane traffic at the trans-Golgi network (TGN) is crucial for correctly distributing various membrane proteins to their destination. Polarly localized auxin efflux proteins, including PIN-FORMED1 (PIN1), are dynamically transported between the endosomes and the plasma membrane (PM) in the plant cells. The intracellular trafficking of PIN1 protein is sensitive to a fungal toxin brefeldin A (BFA), which is known to inhibit guanine-nucleotide exchange factors for ADP ribosylation factors (ARF GEFs) such as GNOM. However, the molecular details of the BFA-sensitive trafficking pathway have not been revealed fully. In a previous study, we have identified an Arabidopsis mutant BFA-visualized endocytic trafficking defective 3 (ben3) which exhibited reduced sensitivity to BFA in terms of BFA-induced intracellular PIN1 agglomeration. Here, we show that BEN3 encodes a member of BIG family ARF GEFs, BIG2. Fluorescent proteins tagged BEN3/BIG2 co-localized with markers for TGN / early endosome (EE). Inspection of conditionally induced de novo synthesized PIN1 confirmed that its secretion to the PM is BFA-sensitive and established BEN3/BIG2 as a crucial component of this BFA action at the level of TGN/EE. Furthermore, ben3 mutation alleviated BFA-induced agglomeration of another TGN-localized ARF GEF BEN1/MIN7. Taken together our results suggest that BEN3/BIG2 is an ARF GEF component, which confers BFA sensitivity to the TGN/EE in Arabidopsis."}],"article_number":"1801-1811","file":[{"checksum":"bd3e3a94d55416739cbb19624bb977f8","date_updated":"2020-07-14T12:48:06Z","access_level":"open_access","file_name":"2017_PlantCellPhysio_Kitakura.pdf","file_size":1352913,"creator":"dernst","date_created":"2019-04-17T07:52:34Z","content_type":"application/pdf","relation":"main_file","file_id":"6333"}],"oa":1,"publication_status":"published","pubrep_id":"1009","volume":58,"file_date_updated":"2020-07-14T12:48:06Z"},{"intvolume":"       174","status":"public","publication":"Plant Physiology","department":[{"_id":"JiFr"}],"quality_controlled":"1","publisher":"American Society of Plant Biologists","date_created":"2018-12-11T11:47:49Z","month":"05","page":"223 - 240","doi":"10.1104/pp.16.01282","ddc":["580"],"language":[{"iso":"eng"}],"pmid":1,"author":[{"last_name":"Synek","first_name":"Lukáš","full_name":"Synek, Lukáš"},{"full_name":"Vukašinović, Nemanja","first_name":"Nemanja","last_name":"Vukašinović"},{"full_name":"Kulich, Ivan","first_name":"Ivan","last_name":"Kulich"},{"last_name":"Hála","first_name":"Michal","full_name":"Hála, Michal"},{"last_name":"Aldorfová","full_name":"Aldorfová, Klára","first_name":"Klára"},{"id":"43905548-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-9767-8699","first_name":"Matyas","full_name":"Fendrych, Matyas","last_name":"Fendrych"},{"full_name":"Žárský, Viktor","first_name":"Viktor","last_name":"Žárský"}],"type":"journal_article","day":"01","title":"EXO70C2 is a key regulatory factor for optimal tip growth of pollen","citation":{"chicago":"Synek, Lukáš, Nemanja Vukašinović, Ivan Kulich, Michal Hála, Klára Aldorfová, Matyas Fendrych, and Viktor Žárský. “EXO70C2 Is a Key Regulatory Factor for Optimal Tip Growth of Pollen.” <i>Plant Physiology</i>. American Society of Plant Biologists, 2017. <a href=\"https://doi.org/10.1104/pp.16.01282\">https://doi.org/10.1104/pp.16.01282</a>.","ieee":"L. Synek <i>et al.</i>, “EXO70C2 is a key regulatory factor for optimal tip growth of pollen,” <i>Plant Physiology</i>, vol. 174, no. 1. American Society of Plant Biologists, pp. 223–240, 2017.","ista":"Synek L, Vukašinović N, Kulich I, Hála M, Aldorfová K, Fendrych M, Žárský V. 2017. EXO70C2 is a key regulatory factor for optimal tip growth of pollen. Plant Physiology. 174(1), 223–240.","mla":"Synek, Lukáš, et al. “EXO70C2 Is a Key Regulatory Factor for Optimal Tip Growth of Pollen.” <i>Plant Physiology</i>, vol. 174, no. 1, American Society of Plant Biologists, 2017, pp. 223–40, doi:<a href=\"https://doi.org/10.1104/pp.16.01282\">10.1104/pp.16.01282</a>.","short":"L. Synek, N. Vukašinović, I. Kulich, M. Hála, K. Aldorfová, M. Fendrych, V. Žárský, Plant Physiology 174 (2017) 223–240.","ama":"Synek L, Vukašinović N, Kulich I, et al. EXO70C2 is a key regulatory factor for optimal tip growth of pollen. <i>Plant Physiology</i>. 2017;174(1):223-240. doi:<a href=\"https://doi.org/10.1104/pp.16.01282\">10.1104/pp.16.01282</a>","apa":"Synek, L., Vukašinović, N., Kulich, I., Hála, M., Aldorfová, K., Fendrych, M., &#38; Žárský, V. (2017). EXO70C2 is a key regulatory factor for optimal tip growth of pollen. <i>Plant Physiology</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1104/pp.16.01282\">https://doi.org/10.1104/pp.16.01282</a>"},"volume":174,"file_date_updated":"2020-07-14T12:47:37Z","oa":1,"publication_status":"published","_id":"669","date_published":"2017-05-01T00:00:00Z","abstract":[{"lang":"eng","text":"The exocyst, a eukaryotic tethering complex, coregulates targeted exocytosis as an effector of small GTPases in polarized cell growth. In land plants, several exocyst subunits are encoded by double or triple paralogs, culminating in tens of EXO70 paralogs. Out of 23 Arabidopsis thaliana EXO70 isoforms, we analyzed seven isoforms expressed in pollen. Genetic and microscopic analyses of single mutants in EXO70A2, EXO70C1, EXO70C2, EXO70F1, EXO70H3, EXO70H5, and EXO70H6 genes revealed that only a loss-of-function EXO70C2 allele resulted in a significant male-specific transmission defect (segregation 40%:51%:9%) due to aberrant pollen tube growth. Mutant pollen tubes grown in vitro exhibited an enhanced growth rate and a decreased thickness of the tip cell wall, causing tip bursts. However, exo70C2 pollen tubes could frequently recover and restart their speedy elongation, resulting in a repetitive stop-and-go growth dynamics. A pollenspecific depletion of the closest paralog, EXO70C1, using artificial microRNA in the exo70C2 mutant background, resulted in a complete pollen-specific transmission defect, suggesting redundant functions of EXO70C1 and EXO70C2. Both EXO70C1 and EXO70C2, GFP tagged and expressed under the control of their native promoters, localized in the cytoplasm of pollen grains, pollen tubes, and also root trichoblast cells. The expression of EXO70C2-GFP complemented the aberrant growth of exo70C2 pollen tubes. The absent EXO70C2 interactions with core exocyst subunits in the yeast two-hybrid assay, cytoplasmic localization, and genetic effect suggest an unconventional EXO70 function possibly as a regulator of exocytosis outside the exocyst complex. In conclusion, EXO70C2 is a novel factor contributing to the regulation of optimal tip growth of Arabidopsis pollen tubes. "}],"file":[{"content_type":"application/pdf","relation":"main_file","file_id":"7041","date_created":"2019-11-18T16:16:18Z","file_name":"2017_PlantPhysio_Synek.pdf","creator":"dernst","file_size":2176903,"checksum":"97155acc6aa5f0d0a78e0589a932fe02","date_updated":"2020-07-14T12:47:37Z","access_level":"open_access"}],"article_processing_charge":"No","issue":"1","publication_identifier":{"issn":["00320889"]},"scopus_import":1,"external_id":{"pmid":["28356503"]},"date_updated":"2021-01-12T08:08:35Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","year":"2017","has_accepted_license":"1","oa_version":"Submitted Version","article_type":"original","publist_id":"7058"},{"title":"Shaping 3D root system architecture","citation":{"apa":"Morris, E., Griffiths, M., Golebiowska, A., Mairhofer, S., Burr Hersey, J., Goh, T., … Bennett, M. (2017). Shaping 3D root system architecture. <i>Current Biology</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cub.2017.06.043\">https://doi.org/10.1016/j.cub.2017.06.043</a>","ama":"Morris E, Griffiths M, Golebiowska A, et al. Shaping 3D root system architecture. <i>Current Biology</i>. 2017;27(17):R919-R930. doi:<a href=\"https://doi.org/10.1016/j.cub.2017.06.043\">10.1016/j.cub.2017.06.043</a>","short":"E. Morris, M. Griffiths, A. Golebiowska, S. Mairhofer, J. Burr Hersey, T. Goh, D. von Wangenheim, B. Atkinson, C. Sturrock, J. Lynch, K. Vissenberg, K. Ritz, D. Wells, S. Mooney, M. Bennett, Current Biology 27 (2017) R919–R930.","mla":"Morris, Emily, et al. “Shaping 3D Root System Architecture.” <i>Current Biology</i>, vol. 27, no. 17, Cell Press, 2017, pp. R919–30, doi:<a href=\"https://doi.org/10.1016/j.cub.2017.06.043\">10.1016/j.cub.2017.06.043</a>.","ieee":"E. Morris <i>et al.</i>, “Shaping 3D root system architecture,” <i>Current Biology</i>, vol. 27, no. 17. Cell Press, pp. R919–R930, 2017.","ista":"Morris E, Griffiths M, Golebiowska A, Mairhofer S, Burr Hersey J, Goh T, von Wangenheim D, Atkinson B, Sturrock C, Lynch J, Vissenberg K, Ritz K, Wells D, Mooney S, Bennett M. 2017. Shaping 3D root system architecture. Current Biology. 27(17), R919–R930.","chicago":"Morris, Emily, Marcus Griffiths, Agata Golebiowska, Stefan Mairhofer, Jasmine Burr Hersey, Tatsuaki Goh, Daniel von Wangenheim, et al. “Shaping 3D Root System Architecture.” <i>Current Biology</i>. Cell Press, 2017. <a href=\"https://doi.org/10.1016/j.cub.2017.06.043\">https://doi.org/10.1016/j.cub.2017.06.043</a>."},"ec_funded":1,"type":"journal_article","author":[{"first_name":"Emily","full_name":"Morris, Emily","last_name":"Morris"},{"full_name":"Griffiths, Marcus","first_name":"Marcus","last_name":"Griffiths"},{"first_name":"Agata","full_name":"Golebiowska, Agata","last_name":"Golebiowska"},{"first_name":"Stefan","full_name":"Mairhofer, Stefan","last_name":"Mairhofer"},{"full_name":"Burr Hersey, Jasmine","first_name":"Jasmine","last_name":"Burr Hersey"},{"last_name":"Goh","full_name":"Goh, Tatsuaki","first_name":"Tatsuaki"},{"last_name":"Von Wangenheim","full_name":"Von Wangenheim, Daniel","first_name":"Daniel","orcid":"0000-0002-6862-1247","id":"49E91952-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Atkinson","full_name":"Atkinson, Brian","first_name":"Brian"},{"last_name":"Sturrock","first_name":"Craig","full_name":"Sturrock, Craig"},{"last_name":"Lynch","full_name":"Lynch, Jonathan","first_name":"Jonathan"},{"full_name":"Vissenberg, Kris","first_name":"Kris","last_name":"Vissenberg"},{"full_name":"Ritz, Karl","first_name":"Karl","last_name":"Ritz"},{"first_name":"Darren","full_name":"Wells, Darren","last_name":"Wells"},{"full_name":"Mooney, Sacha","first_name":"Sacha","last_name":"Mooney"},{"last_name":"Bennett","full_name":"Bennett, Malcolm","first_name":"Malcolm"}],"day":"11","project":[{"_id":"25681D80-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","name":"International IST Postdoc Fellowship Programme","grant_number":"291734"}],"pmid":1,"ddc":["581"],"doi":"10.1016/j.cub.2017.06.043","language":[{"iso":"eng"}],"page":"R919 - R930","date_created":"2018-12-11T11:48:08Z","month":"09","publisher":"Cell Press","intvolume":"        27","status":"public","publication":"Current Biology","department":[{"_id":"JiFr"}],"quality_controlled":"1","oa_version":"Submitted Version","year":"2017","has_accepted_license":"1","publist_id":"6956","publication_identifier":{"issn":["09609822"]},"external_id":{"pmid":["28898665"]},"scopus_import":1,"date_updated":"2021-01-12T08:12:29Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","issue":"17","abstract":[{"lang":"eng","text":"Plants are sessile organisms rooted in one place. The soil resources that plants require are often distributed in a highly heterogeneous pattern. To aid foraging, plants have evolved roots whose growth and development are highly responsive to soil signals. As a result, 3D root architecture is shaped by myriad environmental signals to ensure resource capture is optimised and unfavourable environments are avoided. The first signals sensed by newly germinating seeds — gravity and light — direct root growth into the soil to aid seedling establishment. Heterogeneous soil resources, such as water, nitrogen and phosphate, also act as signals that shape 3D root growth to optimise uptake. Root architecture is also modified through biotic interactions that include soil fungi and neighbouring plants. This developmental plasticity results in a ‘custom-made’ 3D root system that is best adapted to forage for resources in each soil environment that a plant colonises."}],"_id":"722","date_published":"2017-09-11T00:00:00Z","file":[{"relation":"main_file","file_id":"6332","content_type":"application/pdf","date_created":"2019-04-17T07:46:40Z","file_size":1576593,"creator":"dernst","file_name":"2017_CurrentBiology_Morris.pdf","access_level":"open_access","date_updated":"2020-07-14T12:47:54Z","checksum":"e45588b21097b408da6276a3e5eedb2e"}],"oa":1,"publication_status":"published","pubrep_id":"982","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","image":"/images/cc_by_nc_nd.png","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)"},"volume":27,"file_date_updated":"2020-07-14T12:47:54Z"},{"user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","date_updated":"2024-02-12T12:03:42Z","publist_id":"7269","year":"2017","oa_version":"Published Version","has_accepted_license":"1","editor":[{"first_name":"Snježana","full_name":"Jurić, Snježana","last_name":"Jurić"}],"file_date_updated":"2020-07-14T12:46:58Z","pubrep_id":"929","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"publication_status":"published","oa":1,"file":[{"file_size":7443683,"creator":"system","file_name":"IST-2018-929-v1+1_56106.pdf","checksum":"e1f05e5850dfd9f9434d2d373ca61941","access_level":"open_access","date_updated":"2020-07-14T12:46:58Z","relation":"main_file","file_id":"4969","content_type":"application/pdf","date_created":"2018-12-12T10:12:49Z"}],"_id":"545","date_published":"2017-11-17T00:00:00Z","abstract":[{"lang":"eng","text":"Development of vascular tissue is a remarkable example of intercellular communication and coordinated development involving hormonal signaling and tissue polarity. Thus far, studies on vascular patterning and regeneration have been conducted mainly in trees—woody plants—with a well-developed layer of vascular cambium and secondary tissues. Trees are difficult to use as genetic models, i.e., due to long generation time, unstable environmental conditions, and lack of available mutants and transgenic lines. Therefore, the use of the main genetic model plant Arabidopsis thaliana (L.) Heynh., with a wealth of available marker and transgenic lines, provides a unique opportunity to address molecular mechanism of vascular tissue formation and regeneration. With specific treatments, the tiny weed Arabidopsis can serve as a model to understand the growth of mighty trees and interconnect a tree physiology with molecular genetics and cell biology of Arabidopsis."}],"language":[{"iso":"eng"}],"ddc":["581"],"doi":"10.5772/intechopen.69712","project":[{"_id":"25716A02-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","name":"Polarity and subcellular dynamics in plants","grant_number":"282300"}],"related_material":{"record":[{"status":"public","id":"1274","relation":"earlier_version"}]},"day":"17","type":"book_chapter","author":[{"full_name":"Mazur, Ewa","first_name":"Ewa","last_name":"Mazur"},{"last_name":"Friml","full_name":"Friml, Jirí","first_name":"Jirí","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"alternative_title":["Agricultural and Biological Sciences"],"citation":{"short":"E. Mazur, J. Friml, in:, S. Jurić (Ed.), Plant Engineering, InTech, 2017, pp. 113–140.","ama":"Mazur E, Friml J. Vascular tissue development and regeneration in the model plant arabidopsis. In: Jurić S, ed. <i>Plant Engineering</i>. Plant Engineering. InTech; 2017:113-140. doi:<a href=\"https://doi.org/10.5772/intechopen.69712\">10.5772/intechopen.69712</a>","apa":"Mazur, E., &#38; Friml, J. (2017). Vascular tissue development and regeneration in the model plant arabidopsis. In S. Jurić (Ed.), <i>Plant Engineering</i> (pp. 113–140). InTech. <a href=\"https://doi.org/10.5772/intechopen.69712\">https://doi.org/10.5772/intechopen.69712</a>","chicago":"Mazur, Ewa, and Jiří Friml. “Vascular Tissue Development and Regeneration in the Model Plant Arabidopsis.” In <i>Plant Engineering</i>, edited by Snježana Jurić, 113–40. Plant Engineering. InTech, 2017. <a href=\"https://doi.org/10.5772/intechopen.69712\">https://doi.org/10.5772/intechopen.69712</a>.","ista":"Mazur E, Friml J. 2017.Vascular tissue development and regeneration in the model plant arabidopsis. In: Plant Engineering. Agricultural and Biological Sciences, , 113–140.","ieee":"E. Mazur and J. Friml, “Vascular tissue development and regeneration in the model plant arabidopsis,” in <i>Plant Engineering</i>, S. Jurić, Ed. InTech, 2017, pp. 113–140.","mla":"Mazur, Ewa, and Jiří Friml. “Vascular Tissue Development and Regeneration in the Model Plant Arabidopsis.” <i>Plant Engineering</i>, edited by Snježana Jurić, InTech, 2017, pp. 113–40, doi:<a href=\"https://doi.org/10.5772/intechopen.69712\">10.5772/intechopen.69712</a>."},"ec_funded":1,"title":"Vascular tissue development and regeneration in the model plant arabidopsis","publication":"Plant Engineering","quality_controlled":"1","department":[{"_id":"JiFr"}],"status":"public","publisher":"InTech","month":"11","series_title":"Plant Engineering","date_created":"2018-12-11T11:47:05Z","page":"113 - 140"},{"acknowledgement":"fund: FP7-ERC 0101109","project":[{"call_identifier":"FP7","_id":"25681D80-B435-11E9-9278-68D0E5697425","name":"International IST Postdoc Fellowship Programme","grant_number":"291734"}],"related_material":{"record":[{"id":"1078","relation":"research_paper","status":"public"}]},"datarep_id":"66","ddc":["580"],"doi":"10.15479/AT:ISTA:66","date_updated":"2025-05-07T11:12:33Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","title":"Light Sheet Fluorescence microscopy of plant roots growing on the surface of a gel","ec_funded":1,"citation":{"mla":"von Wangenheim, Daniel, et al. <i>Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel</i>. Institute of Science and Technology Austria, 2017, doi:<a href=\"https://doi.org/10.15479/AT:ISTA:66\">10.15479/AT:ISTA:66</a>.","chicago":"Wangenheim, Daniel von, Robert Hauschild, and Jiří Friml. “Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel.” Institute of Science and Technology Austria, 2017. <a href=\"https://doi.org/10.15479/AT:ISTA:66\">https://doi.org/10.15479/AT:ISTA:66</a>.","ista":"von Wangenheim D, Hauschild R, Friml J. 2017. Light Sheet Fluorescence microscopy of plant roots growing on the surface of a gel, Institute of Science and Technology Austria, <a href=\"https://doi.org/10.15479/AT:ISTA:66\">10.15479/AT:ISTA:66</a>.","ieee":"D. von Wangenheim, R. Hauschild, and J. Friml, “Light Sheet Fluorescence microscopy of plant roots growing on the surface of a gel.” Institute of Science and Technology Austria, 2017.","ama":"von Wangenheim D, Hauschild R, Friml J. Light Sheet Fluorescence microscopy of plant roots growing on the surface of a gel. 2017. doi:<a href=\"https://doi.org/10.15479/AT:ISTA:66\">10.15479/AT:ISTA:66</a>","apa":"von Wangenheim, D., Hauschild, R., &#38; Friml, J. (2017). Light Sheet Fluorescence microscopy of plant roots growing on the surface of a gel. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT:ISTA:66\">https://doi.org/10.15479/AT:ISTA:66</a>","short":"D. von Wangenheim, R. Hauschild, J. Friml, (2017)."},"has_accepted_license":"1","oa_version":"Published Version","year":"2017","type":"research_data","author":[{"id":"49E91952-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6862-1247","first_name":"Daniel","full_name":"Von Wangenheim, Daniel","last_name":"Von Wangenheim"},{"orcid":"0000-0001-9843-3522","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","full_name":"Hauschild, Robert","first_name":"Robert","last_name":"Hauschild"},{"first_name":"Jirí","full_name":"Friml, Jirí","last_name":"Friml","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"day":"10","publist_id":"6302","oa":1,"publisher":"Institute of Science and Technology Austria","status":"public","department":[{"_id":"JiFr"},{"_id":"Bio"}],"file_date_updated":"2020-07-14T12:47:03Z","article_processing_charge":"No","_id":"5565","date_created":"2018-12-12T12:31:34Z","date_published":"2017-04-10T00:00:00Z","abstract":[{"lang":"eng","text":"One of the key questions in understanding plant development is how single cells behave in a larger context of the tissue. Therefore, it requires the observation of the whole organ with a high spatial- as well as temporal resolution over prolonged periods of time, which may cause photo-toxic effects. This protocol shows a plant sample preparation method for light-sheet microscopy, which is characterized by mounting the plant vertically on the surface of a gel. The plant is mounted in such a way that the roots are submerged in a liquid medium while the leaves remain in the air. In order to ensure photosynthetic activity of the plant, a custom-made lighting system illuminates the leaves. To keep the roots in darkness the water surface is covered with sheets of black plastic foil. This method allows long-term imaging of plant organ development in standardized conditions. \r\nThe Video is licensed under a CC BY NC ND license. "}],"file":[{"file_size":101497758,"creator":"system","file_name":"IST-2017-66-v1+1_WangenheimHighResolution55044-NEW_1.mp4","access_level":"open_access","date_updated":"2020-07-14T12:47:03Z","checksum":"b7552fc23540a85dc5a22fd4484eae71","file_id":"5599","relation":"main_file","content_type":"video/mp4","date_created":"2018-12-12T13:02:33Z"}],"month":"04"},{"publication":"International Journal of Molecular Sciences","quality_controlled":"1","department":[{"_id":"JiFr"}],"intvolume":"        18","status":"public","publisher":"MDPI","month":"12","date_created":"2018-12-11T11:47:15Z","language":[{"iso":"eng"}],"doi":"10.3390/ijms18122587","ddc":["580"],"day":"01","type":"journal_article","author":[{"first_name":"Damilola","full_name":"Olatunji, Damilola","last_name":"Olatunji"},{"first_name":"Danny","full_name":"Geelen, Danny","last_name":"Geelen"},{"id":"362BF7FE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-7241-2328","last_name":"Verstraeten","full_name":"Verstraeten, Inge","first_name":"Inge"}],"citation":{"short":"D. Olatunji, D. Geelen, I. Verstraeten, International Journal of Molecular Sciences 18 (2017).","apa":"Olatunji, D., Geelen, D., &#38; Verstraeten, I. (2017). Control of endogenous auxin levels in plant root development. <i>International Journal of Molecular Sciences</i>. MDPI. <a href=\"https://doi.org/10.3390/ijms18122587\">https://doi.org/10.3390/ijms18122587</a>","ama":"Olatunji D, Geelen D, Verstraeten I. Control of endogenous auxin levels in plant root development. <i>International Journal of Molecular Sciences</i>. 2017;18(12). doi:<a href=\"https://doi.org/10.3390/ijms18122587\">10.3390/ijms18122587</a>","ieee":"D. Olatunji, D. Geelen, and I. Verstraeten, “Control of endogenous auxin levels in plant root development,” <i>International Journal of Molecular Sciences</i>, vol. 18, no. 12. MDPI, 2017.","ista":"Olatunji D, Geelen D, Verstraeten I. 2017. Control of endogenous auxin levels in plant root development. International Journal of Molecular Sciences. 18(12), 2587.","chicago":"Olatunji, Damilola, Danny Geelen, and Inge Verstraeten. “Control of Endogenous Auxin Levels in Plant Root Development.” <i>International Journal of Molecular Sciences</i>. MDPI, 2017. <a href=\"https://doi.org/10.3390/ijms18122587\">https://doi.org/10.3390/ijms18122587</a>.","mla":"Olatunji, Damilola, et al. “Control of Endogenous Auxin Levels in Plant Root Development.” <i>International Journal of Molecular Sciences</i>, vol. 18, no. 12, 2587, MDPI, 2017, doi:<a href=\"https://doi.org/10.3390/ijms18122587\">10.3390/ijms18122587</a>."},"title":"Control of endogenous auxin levels in plant root development","file_date_updated":"2020-07-14T12:47:10Z","pubrep_id":"917","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"volume":18,"publication_status":"published","oa":1,"article_number":"2587","file":[{"checksum":"82d51f11e493f7eec02976d9a9a9805e","access_level":"open_access","date_updated":"2020-07-14T12:47:10Z","creator":"system","file_size":920962,"file_name":"IST-2017-917-v1+1_ijms-18-02587.pdf","date_created":"2018-12-12T10:08:55Z","file_id":"4718","relation":"main_file","content_type":"application/pdf"}],"date_published":"2017-12-01T00:00:00Z","_id":"572","abstract":[{"lang":"eng","text":"In this review, we summarize the different biosynthesis-related pathways that contribute to the regulation of endogenous auxin in plants. We demonstrate that all known genes involved in auxin biosynthesis also have a role in root formation, from the initiation of a root meristem during embryogenesis to the generation of a functional root system with a primary root, secondary lateral root branches and adventitious roots. Furthermore, the versatile adaptation of root development in response to environmental challenges is mediated by both local and distant control of auxin biosynthesis. In conclusion, auxin homeostasis mediated by spatial and temporal regulation of auxin biosynthesis plays a central role in determining root architecture."}],"article_processing_charge":"No","issue":"12","scopus_import":"1","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","date_updated":"2021-01-12T08:03:16Z","publist_id":"7242","oa_version":"Published Version","has_accepted_license":"1","year":"2017"},{"year":"2017","oa_version":"Submitted Version","publist_id":"7076","publication_identifier":{"issn":["00278424"]},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2021-01-12T08:08:02Z","scopus_import":1,"external_id":{"pmid":["28265057"]},"abstract":[{"lang":"eng","text":"Plant organs are typically organized into three main tissue layers. The middle ground tissue layer comprises the majority of the plant body and serves a wide range of functions, including photosynthesis, selective nutrient uptake and storage, and gravity sensing. Ground tissue patterning and maintenance in Arabidopsis are controlled by a well-established gene network revolving around the key regulator SHORT-ROOT (SHR). In contrast, it is completely unknown how ground tissue identity is first specified from totipotent precursor cells in the embryo. The plant signaling molecule auxin, acting through AUXIN RESPONSE FACTOR (ARF) transcription factors, is critical for embryo patterning. The auxin effector ARF5/MONOPTEROS (MP) acts both cell-autonomously and noncell-autonomously to control embryonic vascular tissue formation and root initiation, respectively. Here we show that auxin response and ARF activity cell-autonomously control the asymmetric division of the first ground tissue cells. By identifying embryonic target genes, we show that MP transcriptionally initiates the ground tissue lineage and acts upstream of the regulatory network that controls ground tissue patterning and maintenance. Strikingly, whereas the SHR network depends on MP, this MP function is, at least in part, SHR independent. Our study therefore identifies auxin response as a regulator of ground tissue specification in the embryonic root, and reveals that ground tissue initiation and maintenance use different regulators and mechanisms. Moreover, our data provide a framework for the simultaneous formation of multiple cell types by the same transcriptional regulator."}],"_id":"657","date_published":"2017-03-21T00:00:00Z","issue":"12","volume":114,"oa":1,"main_file_link":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5373392/","open_access":"1"}],"publication_status":"published","author":[{"last_name":"Möller","full_name":"Möller, Barbara","first_name":"Barbara"},{"full_name":"Ten Hove, Colette","first_name":"Colette","last_name":"Ten Hove"},{"full_name":"Xiang, Daoquan","first_name":"Daoquan","last_name":"Xiang"},{"first_name":"Nerys","full_name":"Williams, Nerys","last_name":"Williams"},{"last_name":"López","full_name":"López, Lorena","first_name":"Lorena"},{"id":"2E46069C-F248-11E8-B48F-1D18A9856A87","first_name":"Saiko","full_name":"Yoshida, Saiko","last_name":"Yoshida"},{"full_name":"Smit, Margot","first_name":"Margot","last_name":"Smit"},{"last_name":"Datla","full_name":"Datla, Raju","first_name":"Raju"},{"full_name":"Weijers, Dolf","first_name":"Dolf","last_name":"Weijers"}],"type":"journal_article","day":"21","title":"Auxin response cell autonomously controls ground tissue initiation in the early arabidopsis embryo","citation":{"chicago":"Möller, Barbara, Colette Ten Hove, Daoquan Xiang, Nerys Williams, Lorena López, Saiko Yoshida, Margot Smit, Raju Datla, and Dolf Weijers. “Auxin Response Cell Autonomously Controls Ground Tissue Initiation in the Early Arabidopsis Embryo.” <i>PNAS</i>. National Academy of Sciences, 2017. <a href=\"https://doi.org/10.1073/pnas.1616493114\">https://doi.org/10.1073/pnas.1616493114</a>.","ista":"Möller B, Ten Hove C, Xiang D, Williams N, López L, Yoshida S, Smit M, Datla R, Weijers D. 2017. Auxin response cell autonomously controls ground tissue initiation in the early arabidopsis embryo. PNAS. 114(12), E2533–E2539.","ieee":"B. Möller <i>et al.</i>, “Auxin response cell autonomously controls ground tissue initiation in the early arabidopsis embryo,” <i>PNAS</i>, vol. 114, no. 12. National Academy of Sciences, pp. E2533–E2539, 2017.","mla":"Möller, Barbara, et al. “Auxin Response Cell Autonomously Controls Ground Tissue Initiation in the Early Arabidopsis Embryo.” <i>PNAS</i>, vol. 114, no. 12, National Academy of Sciences, 2017, pp. E2533–39, doi:<a href=\"https://doi.org/10.1073/pnas.1616493114\">10.1073/pnas.1616493114</a>.","short":"B. Möller, C. Ten Hove, D. Xiang, N. Williams, L. López, S. Yoshida, M. Smit, R. Datla, D. Weijers, PNAS 114 (2017) E2533–E2539.","ama":"Möller B, Ten Hove C, Xiang D, et al. Auxin response cell autonomously controls ground tissue initiation in the early arabidopsis embryo. <i>PNAS</i>. 2017;114(12):E2533-E2539. doi:<a href=\"https://doi.org/10.1073/pnas.1616493114\">10.1073/pnas.1616493114</a>","apa":"Möller, B., Ten Hove, C., Xiang, D., Williams, N., López, L., Yoshida, S., … Weijers, D. (2017). Auxin response cell autonomously controls ground tissue initiation in the early arabidopsis embryo. <i>PNAS</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.1616493114\">https://doi.org/10.1073/pnas.1616493114</a>"},"doi":"10.1073/pnas.1616493114","language":[{"iso":"eng"}],"pmid":1,"date_created":"2018-12-11T11:47:45Z","month":"03","page":"E2533 - E2539","status":"public","intvolume":"       114","department":[{"_id":"JiFr"}],"quality_controlled":"1","publication":"PNAS","publisher":"National Academy of Sciences"},{"month":"06","supervisor":[{"full_name":"Friml, Jiří","first_name":"Jiří","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596"}],"date_created":"2018-12-11T11:49:18Z","page":"117","department":[{"_id":"JiFr"}],"status":"public","publisher":"Institute of Science and Technology Austria","degree_awarded":"PhD","day":"02","type":"dissertation","author":[{"last_name":"Adamowski","full_name":"Adamowski, Maciek","first_name":"Maciek","orcid":"0000-0001-6463-5257","id":"45F536D2-F248-11E8-B48F-1D18A9856A87"}],"alternative_title":["ISTA Thesis"],"citation":{"chicago":"Adamowski, Maciek. “Investigations into Cell Polarity and Trafficking in the Plant Model Arabidopsis Thaliana .” Institute of Science and Technology Austria, 2017. <a href=\"https://doi.org/10.15479/AT:ISTA:th_842\">https://doi.org/10.15479/AT:ISTA:th_842</a>.","ieee":"M. Adamowski, “Investigations into cell polarity and trafficking in the plant model Arabidopsis thaliana ,” Institute of Science and Technology Austria, 2017.","ista":"Adamowski M. 2017. Investigations into cell polarity and trafficking in the plant model Arabidopsis thaliana . Institute of Science and Technology Austria.","mla":"Adamowski, Maciek. <i>Investigations into Cell Polarity and Trafficking in the Plant Model Arabidopsis Thaliana </i>. Institute of Science and Technology Austria, 2017, doi:<a href=\"https://doi.org/10.15479/AT:ISTA:th_842\">10.15479/AT:ISTA:th_842</a>.","short":"M. Adamowski, Investigations into Cell Polarity and Trafficking in the Plant Model Arabidopsis Thaliana , Institute of Science and Technology Austria, 2017.","ama":"Adamowski M. Investigations into cell polarity and trafficking in the plant model Arabidopsis thaliana . 2017. doi:<a href=\"https://doi.org/10.15479/AT:ISTA:th_842\">10.15479/AT:ISTA:th_842</a>","apa":"Adamowski, M. (2017). <i>Investigations into cell polarity and trafficking in the plant model Arabidopsis thaliana </i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT:ISTA:th_842\">https://doi.org/10.15479/AT:ISTA:th_842</a>"},"title":"Investigations into cell polarity and trafficking in the plant model Arabidopsis thaliana ","language":[{"iso":"eng"}],"ddc":["581","583","580"],"doi":"10.15479/AT:ISTA:th_842","related_material":{"record":[{"status":"public","relation":"part_of_dissertation","id":"1591"}]},"file":[{"date_created":"2019-04-05T09:03:20Z","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","file_id":"6215","relation":"source_file","checksum":"193425764d9aaaed3ac57062a867b315","date_updated":"2020-07-14T12:48:15Z","access_level":"closed","file_name":"2017_Adamowski-Thesis_Source.docx","creator":"dernst","file_size":46903863},{"relation":"main_file","file_id":"6216","content_type":"application/pdf","date_created":"2019-04-05T09:03:19Z","file_size":8698888,"creator":"dernst","file_name":"2017_Adamowski-Thesis.pdf","access_level":"open_access","date_updated":"2020-07-14T12:48:15Z","checksum":"df5ab01be81f821e1b958596a1ec8d21"}],"_id":"938","abstract":[{"lang":"eng","text":"The thesis encompasses several topics of plant cell biology which were studied in the model plant Arabidopsis thaliana. Chapter 1 concerns the plant hormone auxin and its polar transport through cells and tissues. The highly controlled, directional transport of auxin is facilitated by plasma membrane-localized transporters. Transporters from the PIN family direct auxin transport due to their polarized localizations at cell membranes. Substantial effort has been put into research on cellular trafficking of PIN proteins, which is thought to underlie their polar distribution. I participated in a forward genetic screen aimed at identifying novel regulators of PIN polarity. The screen yielded several genes which may be involved in PIN polarity regulation or participate in polar auxin transport by other means. Chapter 2 focuses on the endomembrane system, with particular attention to clathrin-mediated endocytosis. The project started with identification of several proteins that interact with clathrin light chains. Among them, I focused on two putative homologues of auxilin, which in non-plant systems is an endocytotic factor known for uncoating clathrin-coated vesicles in the final step of endocytosis. The body of my work consisted of an in-depth characterization of transgenic A. thaliana lines overexpressing these putative auxilins in an inducible manner. Overexpression of these proteins leads to an inhibition of endocytosis, as documented by imaging of cargoes and clathrin-related endocytic machinery. An extension of this work is an investigation into a concept of homeostatic regulation acting between distinct transport processes in the endomembrane system. With auxilin overexpressing lines, where endocytosis is blocked specifically, I made observations on the mutual relationship between two opposite trafficking processes of secretion and endocytosis. In Chapter 3, I analyze cortical microtubule arrays and their relationship to auxin signaling and polarized growth in elongating cells. In plants, microtubules are organized into arrays just below the plasma membrane, and it is thought that their function is to guide membrane-docked cellulose synthase complexes. These, in turn, influence cell wall structure and cell shape by directed deposition of cellulose fibres. In elongating cells, cortical microtubule arrays are able to reorient in relation to long cell axis, and these reorientations have been linked to cell growth and to signaling of growth-regulating factors such as auxin or light. In this chapter, I am addressing the causal relationship between microtubule array reorientation, growth, and auxin signaling. I arrive at a model where array reorientation is not guided by auxin directly, but instead is only controlled by growth, which, in turn, is regulated by auxin."}],"date_published":"2017-06-02T00:00:00Z","article_processing_charge":"No","file_date_updated":"2020-07-14T12:48:15Z","pubrep_id":"842","publication_status":"published","oa":1,"publist_id":"6483","has_accepted_license":"1","oa_version":"Published Version","year":"2017","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","date_updated":"2023-09-07T12:06:09Z","publication_identifier":{"issn":["2663-337X"]}},{"article_processing_charge":"Yes","file":[{"content_type":"application/pdf","file_id":"5315","relation":"main_file","date_created":"2018-12-12T10:17:57Z","file_name":"IST-2017-847-v1+1_elife-26792-v2.pdf","file_size":19581847,"creator":"system","date_updated":"2020-07-14T12:48:15Z","access_level":"open_access","checksum":"9af3398cb0d81f99d79016a616df22e9"}],"article_number":"e26792","date_published":"2017-06-19T00:00:00Z","_id":"946","abstract":[{"text":"Roots navigate through soil integrating environmental signals to orient their growth. The Arabidopsis root is a widely used model for developmental, physiological and cell biological studies. Live imaging greatly aids these efforts, but the horizontal sample position and continuous root tip displacement present significant difficulties. Here, we develop a confocal microscope setup for vertical sample mounting and integrated directional illumination. We present TipTracker – a custom software for automatic tracking of diverse moving objects usable on various microscope setups. Combined, this enables observation of root tips growing along the natural gravity vector over prolonged periods of time, as well as the ability to induce rapid gravity or light stimulation. We also track migrating cells in the developing zebrafish embryo, demonstrating the utility of this system in the acquisition of high-resolution data sets of dynamic samples. We provide detailed descriptions of the tools enabling the easy implementation on other microscopes.","lang":"eng"}],"publication_status":"published","oa":1,"file_date_updated":"2020-07-14T12:48:15Z","pubrep_id":"847","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"volume":6,"publist_id":"6471","oa_version":"Published Version","has_accepted_license":"1","year":"2017","scopus_import":"1","external_id":{"isi":["000404728300001"]},"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","date_updated":"2025-05-07T11:12:33Z","acknowledged_ssus":[{"_id":"M-Shop"},{"_id":"Bio"}],"month":"06","date_created":"2018-12-11T11:49:21Z","publisher":"eLife Sciences Publications","isi":1,"publication":"eLife","quality_controlled":"1","department":[{"_id":"JiFr"},{"_id":"Bio"},{"_id":"CaHe"},{"_id":"EvBe"}],"intvolume":"         6","status":"public","citation":{"chicago":"Wangenheim, Daniel von, Robert Hauschild, Matyas Fendrych, Vanessa Barone, Eva Benková, and Jiří Friml. “Live Tracking of Moving Samples in Confocal Microscopy for Vertically Grown Roots.” <i>ELife</i>. eLife Sciences Publications, 2017. <a href=\"https://doi.org/10.7554/eLife.26792\">https://doi.org/10.7554/eLife.26792</a>.","ista":"von Wangenheim D, Hauschild R, Fendrych M, Barone V, Benková E, Friml J. 2017. Live tracking of moving samples in confocal microscopy for vertically grown roots. eLife. 6, e26792.","ieee":"D. von Wangenheim, R. Hauschild, M. Fendrych, V. Barone, E. Benková, and J. Friml, “Live tracking of moving samples in confocal microscopy for vertically grown roots,” <i>eLife</i>, vol. 6. eLife Sciences Publications, 2017.","mla":"von Wangenheim, Daniel, et al. “Live Tracking of Moving Samples in Confocal Microscopy for Vertically Grown Roots.” <i>ELife</i>, vol. 6, e26792, eLife Sciences Publications, 2017, doi:<a href=\"https://doi.org/10.7554/eLife.26792\">10.7554/eLife.26792</a>.","short":"D. von Wangenheim, R. Hauschild, M. Fendrych, V. Barone, E. Benková, J. Friml, ELife 6 (2017).","ama":"von Wangenheim D, Hauschild R, Fendrych M, Barone V, Benková E, Friml J. Live tracking of moving samples in confocal microscopy for vertically grown roots. <i>eLife</i>. 2017;6. doi:<a href=\"https://doi.org/10.7554/eLife.26792\">10.7554/eLife.26792</a>","apa":"von Wangenheim, D., Hauschild, R., Fendrych, M., Barone, V., Benková, E., &#38; Friml, J. (2017). Live tracking of moving samples in confocal microscopy for vertically grown roots. <i>ELife</i>. eLife Sciences Publications. <a href=\"https://doi.org/10.7554/eLife.26792\">https://doi.org/10.7554/eLife.26792</a>"},"ec_funded":1,"title":"Live tracking of moving samples in confocal microscopy for vertically grown roots","day":"19","type":"journal_article","author":[{"id":"49E91952-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6862-1247","first_name":"Daniel","full_name":"Von Wangenheim, Daniel","last_name":"Von Wangenheim"},{"orcid":"0000-0001-9843-3522","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild","full_name":"Hauschild, Robert","first_name":"Robert"},{"first_name":"Matyas","full_name":"Fendrych, Matyas","last_name":"Fendrych","id":"43905548-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-9767-8699"},{"orcid":"0000-0003-2676-3367","id":"419EECCC-F248-11E8-B48F-1D18A9856A87","full_name":"Barone, Vanessa","first_name":"Vanessa","last_name":"Barone"},{"first_name":"Eva","full_name":"Benková, Eva","last_name":"Benková","orcid":"0000-0002-8510-9739","id":"38F4F166-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Friml","first_name":"Jirí","full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"related_material":{"record":[{"id":"5566","relation":"popular_science","status":"public"}]},"project":[{"name":"International IST Postdoc Fellowship Programme","grant_number":"291734","call_identifier":"FP7","_id":"25681D80-B435-11E9-9278-68D0E5697425"},{"name":"Molecular basis of root growth inhibition by auxin","grant_number":"M02128","_id":"2572ED28-B435-11E9-9278-68D0E5697425","call_identifier":"FWF"},{"_id":"2542D156-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","grant_number":"I 1774-B16","name":"Hormone cross-talk drives nutrient dependent plant development"},{"call_identifier":"FP7","_id":"25716A02-B435-11E9-9278-68D0E5697425","grant_number":"282300","name":"Polarity and subcellular dynamics in plants"}],"acknowledgement":"Funding: Marie Curie Actions (FP7/2007-2013 no 291734) to Daniel von Wangenheim; Austrian Science Fund (M 2128-B21) to Matyáš Fendrych; Austrian Science Fund (FWF01_I1774S) to Eva Benková; European Research Council (FP7/2007-2013 no 282300) to Jiří Friml. \r\nThe authors are grateful to the Miba Machine Shop at IST Austria for their contribution to the microscope setup and to Yvonne Kemper for reading, understanding and correcting the manuscript.\r\n#BioimagingFacility","language":[{"iso":"eng"}],"ddc":["570"],"doi":"10.7554/eLife.26792"},{"acknowledgement":"We thank Bonnie Bartel, Jenny Russinova and Niko Geldner\r\nfor sharing published material, Martine de Cock and Annick\r\nBleys for help in preparing the manuscript. This work was\r\nsupported by the European Research Council (project\r\nERC-2011-StG-20101109-PSDP); Czech Science Foundation\r\nGAČR (GA13-40637S); project CEITEC—Central European\r\nInstitute of Technology (CZ.1.05/1.1.00/02.0068). SV is a\r\npostdoctoral fellow of the Research Foundation-Flanders.\r\nSN is a Project Assistant Professor supported by the Japanese\r\nSociety for the Promotion of Science (JSPS; 30612022 to SN),\r\nthe NC-CARP project of the Ministry of Education, Culture,\r\nSports, Science and Technology in Japan to SN.","project":[{"name":"Polarity and subcellular dynamics in plants","grant_number":"282300","_id":"25716A02-B435-11E9-9278-68D0E5697425","call_identifier":"FP7"}],"doi":"10.1038/celldisc.2016.18","ddc":["580"],"language":[{"iso":"eng"}],"title":"Cellular mechanisms for cargo delivery and polarity maintenance at different polar domains in plant cells","ec_funded":1,"citation":{"short":"Ł. Łangowski, K.T. Wabnik, H. Li, S. Vanneste, S. Naramoto, H. Tanaka, J. Friml, Cell Discovery 2 (2016).","ama":"Łangowski Ł, Wabnik KT, Li H, et al. Cellular mechanisms for cargo delivery and polarity maintenance at different polar domains in plant cells. <i>Cell Discovery</i>. 2016;2. doi:<a href=\"https://doi.org/10.1038/celldisc.2016.18\">10.1038/celldisc.2016.18</a>","apa":"Łangowski, Ł., Wabnik, K. T., Li, H., Vanneste, S., Naramoto, S., Tanaka, H., &#38; Friml, J. (2016). Cellular mechanisms for cargo delivery and polarity maintenance at different polar domains in plant cells. <i>Cell Discovery</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/celldisc.2016.18\">https://doi.org/10.1038/celldisc.2016.18</a>","chicago":"Łangowski, Łukasz, Krzysztof T Wabnik, Hongjiang Li, Steffen Vanneste, Satoshi Naramoto, Hirokazu Tanaka, and Jiří Friml. “Cellular Mechanisms for Cargo Delivery and Polarity Maintenance at Different Polar Domains in Plant Cells.” <i>Cell Discovery</i>. Nature Publishing Group, 2016. <a href=\"https://doi.org/10.1038/celldisc.2016.18\">https://doi.org/10.1038/celldisc.2016.18</a>.","ieee":"Ł. Łangowski <i>et al.</i>, “Cellular mechanisms for cargo delivery and polarity maintenance at different polar domains in plant cells,” <i>Cell Discovery</i>, vol. 2. Nature Publishing Group, 2016.","ista":"Łangowski Ł, Wabnik KT, Li H, Vanneste S, Naramoto S, Tanaka H, Friml J. 2016. Cellular mechanisms for cargo delivery and polarity maintenance at different polar domains in plant cells. Cell Discovery. 2, 16018.","mla":"Łangowski, Łukasz, et al. “Cellular Mechanisms for Cargo Delivery and Polarity Maintenance at Different Polar Domains in Plant Cells.” <i>Cell Discovery</i>, vol. 2, 16018, Nature Publishing Group, 2016, doi:<a href=\"https://doi.org/10.1038/celldisc.2016.18\">10.1038/celldisc.2016.18</a>."},"type":"journal_article","author":[{"first_name":"Łukasz","full_name":"Łangowski, Łukasz","last_name":"Łangowski"},{"last_name":"Wabnik","full_name":"Wabnik, Krzysztof T","first_name":"Krzysztof T","orcid":"0000-0001-7263-0560","id":"4DE369A4-F248-11E8-B48F-1D18A9856A87"},{"id":"33CA54A6-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5039-9660","last_name":"Li","first_name":"Hongjiang","full_name":"Li, Hongjiang"},{"last_name":"Vanneste","first_name":"Steffen","full_name":"Vanneste, Steffen"},{"last_name":"Naramoto","first_name":"Satoshi","full_name":"Naramoto, Satoshi"},{"last_name":"Tanaka","first_name":"Hirokazu","full_name":"Tanaka, Hirokazu"},{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jirí","full_name":"Friml, Jirí","last_name":"Friml"}],"day":"19","publisher":"Nature Publishing Group","status":"public","intvolume":"         2","quality_controlled":"1","department":[{"_id":"EvBe"},{"_id":"JiFr"}],"publication":"Cell Discovery","date_created":"2018-12-11T11:50:02Z","month":"07","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","date_updated":"2021-01-12T06:48:08Z","scopus_import":1,"year":"2016","has_accepted_license":"1","oa_version":"Published Version","publist_id":"6299","oa":1,"publication_status":"published","volume":2,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"pubrep_id":"757","file_date_updated":"2018-12-12T10:13:33Z","abstract":[{"lang":"eng","text":"The asymmetric localization of proteins in the plasma membrane domains of eukaryotic cells is a fundamental manifestation of cell polarity that is central to multicellular organization and developmental patterning. In plants, the mechanisms underlying the polar localization of cargo proteins are still largely unknown and appear to be fundamentally distinct from those operating in mammals. Here, we present a systematic, quantitative comparative analysis of the polar delivery and subcellular localization of proteins that characterize distinct polar plasma membrane domains in plant cells. The combination of microscopic analyses and computational modeling revealed a mechanistic framework common to diverse polar cargos and underlying the establishment and maintenance of apical, basal, and lateral polar domains in plant cells. This mechanism depends on the polar secretion, constitutive endocytic recycling, and restricted lateral diffusion of cargos within the plasma membrane. Moreover, our observations suggest that polar cargo distribution involves the individual protein potential to form clusters within the plasma membrane and interact with the extracellular matrix. Our observations provide insights into the shared cellular mechanisms of polar cargo delivery and polarity maintenance in plant cells."}],"_id":"1081","date_published":"2016-07-19T00:00:00Z","article_number":"16018","file":[{"content_type":"application/pdf","file_id":"5017","relation":"main_file","date_created":"2018-12-12T10:13:33Z","file_name":"IST-2017-757-v1+1_celldisc201618.pdf","creator":"system","file_size":5261671,"date_updated":"2018-12-12T10:13:33Z","access_level":"open_access"}]}]