[{"day":"06","type":"journal_article","author":[{"first_name":"E","full_name":"Lee, E","last_name":"Lee"},{"first_name":"B","full_name":"Vila Nova Santana, B","last_name":"Vila Nova Santana"},{"last_name":"Samuels","full_name":"Samuels, E","first_name":"E"},{"first_name":"F","full_name":"Benitez-Fuente, F","last_name":"Benitez-Fuente"},{"full_name":"Corsi, E","first_name":"E","last_name":"Corsi"},{"full_name":"Botella, MA","first_name":"MA","last_name":"Botella"},{"last_name":"Perez-Sancho","first_name":"J","full_name":"Perez-Sancho, J"},{"first_name":"S","full_name":"Vanneste, S","last_name":"Vanneste"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","last_name":"Friml","first_name":"Jiří","full_name":"Friml, Jiří"},{"full_name":"Macho, A","first_name":"A","last_name":"Macho"},{"first_name":"A","full_name":"Alves Azevedo, A","last_name":"Alves Azevedo"},{"first_name":"A","full_name":"Rosado, A","last_name":"Rosado"}],"citation":{"short":"E. Lee, B. Vila Nova Santana, E. Samuels, F. Benitez-Fuente, E. Corsi, M. Botella, J. Perez-Sancho, S. Vanneste, J. Friml, A. Macho, A. Alves Azevedo, A. Rosado, Journal of Experimental Botany 71 (2020) 3986–3998.","ama":"Lee E, Vila Nova Santana B, Samuels E, et al. Rare earth elements induce cytoskeleton-dependent and PI4P-associated rearrangement of SYT1/SYT5 ER-PM contact site complexes in Arabidopsis. <i>Journal of Experimental Botany</i>. 2020;71(14):3986–3998. doi:<a href=\"https://doi.org/10.1093/jxb/eraa138\">10.1093/jxb/eraa138</a>","apa":"Lee, E., Vila Nova Santana, B., Samuels, E., Benitez-Fuente, F., Corsi, E., Botella, M., … Rosado, A. (2020). Rare earth elements induce cytoskeleton-dependent and PI4P-associated rearrangement of SYT1/SYT5 ER-PM contact site complexes in Arabidopsis. <i>Journal of Experimental Botany</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/jxb/eraa138\">https://doi.org/10.1093/jxb/eraa138</a>","chicago":"Lee, E, B Vila Nova Santana, E Samuels, F Benitez-Fuente, E Corsi, MA Botella, J Perez-Sancho, et al. “Rare Earth Elements Induce Cytoskeleton-Dependent and PI4P-Associated Rearrangement of SYT1/SYT5 ER-PM Contact Site Complexes in Arabidopsis.” <i>Journal of Experimental Botany</i>. Oxford University Press, 2020. <a href=\"https://doi.org/10.1093/jxb/eraa138\">https://doi.org/10.1093/jxb/eraa138</a>.","ieee":"E. Lee <i>et al.</i>, “Rare earth elements induce cytoskeleton-dependent and PI4P-associated rearrangement of SYT1/SYT5 ER-PM contact site complexes in Arabidopsis,” <i>Journal of Experimental Botany</i>, vol. 71, no. 14. Oxford University Press, pp. 3986–3998, 2020.","ista":"Lee E, Vila Nova Santana B, Samuels E, Benitez-Fuente F, Corsi E, Botella M, Perez-Sancho J, Vanneste S, Friml J, Macho A, Alves Azevedo A, Rosado A. 2020. Rare earth elements induce cytoskeleton-dependent and PI4P-associated rearrangement of SYT1/SYT5 ER-PM contact site complexes in Arabidopsis. Journal of Experimental Botany. 71(14), 3986–3998.","mla":"Lee, E., et al. “Rare Earth Elements Induce Cytoskeleton-Dependent and PI4P-Associated Rearrangement of SYT1/SYT5 ER-PM Contact Site Complexes in Arabidopsis.” <i>Journal of Experimental Botany</i>, vol. 71, no. 14, Oxford University Press, 2020, pp. 3986–3998, doi:<a href=\"https://doi.org/10.1093/jxb/eraa138\">10.1093/jxb/eraa138</a>."},"title":"Rare earth elements induce cytoskeleton-dependent and PI4P-associated rearrangement of SYT1/SYT5 ER-PM contact site complexes in Arabidopsis","language":[{"iso":"eng"}],"ddc":["580"],"doi":"10.1093/jxb/eraa138","pmid":1,"month":"07","date_created":"2020-04-06T10:57:08Z","page":"3986–3998","publication":"Journal of Experimental Botany","quality_controlled":"1","department":[{"_id":"JiFr"}],"status":"public","intvolume":"        71","publisher":"Oxford University Press","isi":1,"article_type":"original","year":"2020","has_accepted_license":"1","oa_version":"Published Version","external_id":{"isi":["000553125400007"],"pmid":["32179893"]},"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","date_updated":"2023-08-18T10:27:52Z","publication_identifier":{"eissn":["1460-2431"],"issn":["0022-0957"]},"file":[{"relation":"main_file","file_id":"8613","content_type":"application/pdf","success":1,"date_created":"2020-10-06T07:41:35Z","creator":"dernst","file_size":1916031,"file_name":"2020_JourExperimBotany_Lee.pdf","access_level":"open_access","date_updated":"2020-10-06T07:41:35Z","checksum":"b06aaaa93dc41896da805fe4b75cf3a1"}],"abstract":[{"text":"In plant cells, environmental stressors promote changes in connectivity between the cortical ER and the PM. Although this process is tightly regulated in space and time, the molecular signals and structural components mediating these changes in inter-organelle communication are only starting to be characterized. In this report, we confirm the presence of a putative tethering complex containing the synaptotagmins 1 and 5 (SYT1 and SYT5) and the Ca2+ and lipid binding protein 1 (CLB1/SYT7). This complex is enriched at ER-PM contact sites (EPCS), have slow responses to changes in extracellular Ca2+, and display severe cytoskeleton-dependent rearrangements in response to the trivalent lanthanum (La3+) and gadolinium (Gd3+) rare earth elements (REEs). Although REEs are generally used as non-selective cation channel blockers at the PM, here we show that the slow internalization of REEs into the cytosol underlies the activation of the Ca2+/Calmodulin intracellular signaling, the accumulation of phosphatidylinositol-4-phosphate (PI4P) at the PM, and the cytoskeleton-dependent rearrangement of the SYT1/SYT5 EPCS complexes. We propose that the observed EPCS rearrangements act as a slow adaptive response to sustained stress conditions, and that this process involves the accumulation of stress-specific phosphoinositides species at the PM.","lang":"eng"}],"_id":"7646","date_published":"2020-07-06T00:00:00Z","article_processing_charge":"No","issue":"14","file_date_updated":"2020-10-06T07:41:35Z","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"volume":71,"publication_status":"published","oa":1},{"citation":{"mla":"Xue, Huidan, et al. “Neo-Gibberellin Signaling: Guiding the next Generation of the Green Revolution.” <i>Trends in Plant Science</i>, vol. 25, no. 6, Elsevier, 2020, pp. 520–22, doi:<a href=\"https://doi.org/10.1016/j.tplants.2020.04.001\">10.1016/j.tplants.2020.04.001</a>.","ieee":"H. Xue, Y. Zhang, and G. Xiao, “Neo-gibberellin signaling: Guiding the next generation of the green revolution,” <i>Trends in Plant Science</i>, vol. 25, no. 6. Elsevier, pp. 520–522, 2020.","ista":"Xue H, Zhang Y, Xiao G. 2020. Neo-gibberellin signaling: Guiding the next generation of the green revolution. Trends in Plant Science. 25(6), 520–522.","chicago":"Xue, Huidan, Yuzhou Zhang, and Guanghui Xiao. “Neo-Gibberellin Signaling: Guiding the next Generation of the Green Revolution.” <i>Trends in Plant Science</i>. Elsevier, 2020. <a href=\"https://doi.org/10.1016/j.tplants.2020.04.001\">https://doi.org/10.1016/j.tplants.2020.04.001</a>.","apa":"Xue, H., Zhang, Y., &#38; Xiao, G. (2020). Neo-gibberellin signaling: Guiding the next generation of the green revolution. <i>Trends in Plant Science</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.tplants.2020.04.001\">https://doi.org/10.1016/j.tplants.2020.04.001</a>","ama":"Xue H, Zhang Y, Xiao G. Neo-gibberellin signaling: Guiding the next generation of the green revolution. <i>Trends in Plant Science</i>. 2020;25(6):520-522. doi:<a href=\"https://doi.org/10.1016/j.tplants.2020.04.001\">10.1016/j.tplants.2020.04.001</a>","short":"H. Xue, Y. Zhang, G. Xiao, Trends in Plant Science 25 (2020) 520–522."},"title":"Neo-gibberellin signaling: Guiding the next generation of the green revolution","day":"01","type":"journal_article","author":[{"last_name":"Xue","first_name":"Huidan","full_name":"Xue, Huidan"},{"full_name":"Zhang, Yuzhou","first_name":"Yuzhou","last_name":"Zhang","id":"3B6137F2-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2627-6956"},{"last_name":"Xiao","first_name":"Guanghui","full_name":"Xiao, Guanghui"}],"pmid":1,"language":[{"iso":"eng"}],"doi":"10.1016/j.tplants.2020.04.001","page":"520-522","month":"06","date_created":"2020-04-26T22:00:46Z","publisher":"Elsevier","isi":1,"publication":"Trends in Plant Science","quality_controlled":"1","department":[{"_id":"JiFr"}],"status":"public","intvolume":"        25","article_type":"original","year":"2020","oa_version":"None","external_id":{"isi":["000533518400003"],"pmid":["32407691"]},"scopus_import":"1","date_updated":"2023-08-21T06:16:01Z","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","publication_identifier":{"issn":["1360-1385"]},"article_processing_charge":"No","issue":"6","_id":"7686","abstract":[{"lang":"eng","text":"The agricultural green revolution spectacularly enhanced crop yield and lodging resistance with modified DELLA-mediated gibberellin signaling. However, this was achieved at the expense of reduced nitrogen-use efficiency (NUE). Recently, Wu et al. revealed novel gibberellin signaling that provides a blueprint for improving tillering and NUE in Green Revolution varieties (GRVs). "}],"date_published":"2020-06-01T00:00:00Z","publication_status":"published","volume":25},{"publication_status":"published","oa":1,"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2020.02.13.948109"}],"volume":183,"issue":"3","article_processing_charge":"No","abstract":[{"lang":"eng","text":"The TPLATE complex (TPC) is a key endocytic adaptor protein complex in plants. TPC in Arabidopsis (Arabidopsis thaliana) contains six evolutionarily conserved subunits and two plant-specific subunits, AtEH1/Pan1 and AtEH2/Pan1, although cytoplasmic proteins are not associated with the hexameric subcomplex in the cytoplasm. To investigate the dynamic assembly of the octameric TPC at the plasma membrane (PM), we performed state-of-the-art dual-color live cell imaging at physiological and lowered temperatures. Lowering the temperature slowed down endocytosis, thereby enhancing the temporal resolution of the differential recruitment of endocytic components. Under both normal and lowered temperature conditions, the core TPC subunit TPLATE and the AtEH/Pan1 proteins exhibited simultaneous recruitment at the PM. These results, together with co-localization analysis of different TPC subunits, allow us to conclude that TPC in plant cells is not recruited to the PM sequentially but as an octameric complex."}],"_id":"7695","date_published":"2020-07-01T00:00:00Z","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","date_updated":"2023-09-05T12:20:02Z","external_id":{"pmid":["32321842"],"isi":["000550682000018"]},"scopus_import":"1","publication_identifier":{"issn":["0032-0889"],"eissn":["1532-2548"]},"article_type":"original","year":"2020","oa_version":"Preprint","publisher":"American Society of Plant Biologists","isi":1,"quality_controlled":"1","department":[{"_id":"JiFr"}],"publication":"Plant Physiology","status":"public","intvolume":"       183","page":"986-997","month":"07","date_created":"2020-04-29T15:23:00Z","project":[{"_id":"26538374-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","name":"Molecular mechanisms of endocytic cargo recognition in plants","grant_number":"I03630"}],"pmid":1,"language":[{"iso":"eng"}],"doi":"10.1104/pp.20.00178","citation":{"mla":"Wang, J., et al. “High Temporal Resolution Reveals Simultaneous Plasma Membrane Recruitment of TPLATE Complex Subunits.” <i>Plant Physiology</i>, vol. 183, no. 3, American Society of Plant Biologists, 2020, pp. 986–97, doi:<a href=\"https://doi.org/10.1104/pp.20.00178\">10.1104/pp.20.00178</a>.","chicago":"Wang, J, E Mylle, Alexander J Johnson, N Besbrugge, G De Jaeger, Jiří Friml, R Pleskot, and D van Damme. “High Temporal Resolution Reveals Simultaneous Plasma Membrane Recruitment of TPLATE Complex Subunits.” <i>Plant Physiology</i>. American Society of Plant Biologists, 2020. <a href=\"https://doi.org/10.1104/pp.20.00178\">https://doi.org/10.1104/pp.20.00178</a>.","ista":"Wang J, Mylle E, Johnson AJ, Besbrugge N, De Jaeger G, Friml J, Pleskot R, van Damme D. 2020. High temporal resolution reveals simultaneous plasma membrane recruitment of TPLATE complex subunits. Plant Physiology. 183(3), 986–997.","ieee":"J. Wang <i>et al.</i>, “High temporal resolution reveals simultaneous plasma membrane recruitment of TPLATE complex subunits,” <i>Plant Physiology</i>, vol. 183, no. 3. American Society of Plant Biologists, pp. 986–997, 2020.","ama":"Wang J, Mylle E, Johnson AJ, et al. High temporal resolution reveals simultaneous plasma membrane recruitment of TPLATE complex subunits. <i>Plant Physiology</i>. 2020;183(3):986-997. doi:<a href=\"https://doi.org/10.1104/pp.20.00178\">10.1104/pp.20.00178</a>","apa":"Wang, J., Mylle, E., Johnson, A. J., Besbrugge, N., De Jaeger, G., Friml, J., … van Damme, D. (2020). High temporal resolution reveals simultaneous plasma membrane recruitment of TPLATE complex subunits. <i>Plant Physiology</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1104/pp.20.00178\">https://doi.org/10.1104/pp.20.00178</a>","short":"J. Wang, E. Mylle, A.J. Johnson, N. Besbrugge, G. De Jaeger, J. Friml, R. Pleskot, D. van Damme, Plant Physiology 183 (2020) 986–997."},"title":"High temporal resolution reveals simultaneous plasma membrane recruitment of TPLATE complex subunits","day":"01","author":[{"full_name":"Wang, J","first_name":"J","last_name":"Wang"},{"last_name":"Mylle","full_name":"Mylle, E","first_name":"E"},{"id":"46A62C3A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-2739-8843","full_name":"Johnson, Alexander J","first_name":"Alexander J","last_name":"Johnson"},{"last_name":"Besbrugge","full_name":"Besbrugge, N","first_name":"N"},{"first_name":"G","full_name":"De Jaeger, G","last_name":"De Jaeger"},{"last_name":"Friml","first_name":"Jiří","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596"},{"last_name":"Pleskot","first_name":"R","full_name":"Pleskot, R"},{"full_name":"van Damme, D","first_name":"D","last_name":"van Damme"}],"type":"journal_article"},{"has_accepted_license":"1","year":"2020","oa_version":"Published Version","article_type":"original","publication_identifier":{"issn":["0028-646X"],"eissn":["1469-8137"]},"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","date_updated":"2023-09-05T15:46:04Z","scopus_import":"1","external_id":{"pmid":["32350870"],"isi":["000534092400001"]},"issue":"5","article_processing_charge":"Yes (via OA deal)","abstract":[{"text":"* Morphogenesis and adaptive tropic growth in plants depend on gradients of the phytohormone auxin, mediated by the membrane‐based PIN‐FORMED (PIN) auxin transporters. PINs localize to a particular side of the plasma membrane (PM) or to the endoplasmic reticulum (ER) to directionally transport auxin and maintain intercellular and intracellular auxin homeostasis, respectively. However, the molecular cues that confer their diverse cellular localizations remain largely unknown.\r\n* In this study, we systematically swapped the domains between ER‐ and PM‐localized PIN proteins, as well as between apical and basal PM‐localized PINs from Arabidopsis thaliana , to shed light on why PIN family members with similar topological structures reside at different membrane compartments within cells.\r\n* Our results show that not only do the N‐ and C‐terminal transmembrane domains (TMDs) and central hydrophilic loop contribute to their differential subcellular localizations and cellular polarity, but that the pairwise‐matched N‐ and C‐terminal TMDs resulting from intramolecular domain–domain coevolution are also crucial for their divergent patterns of localization.\r\n* These findings illustrate the complexity of the evolutionary path of PIN proteins in acquiring their plethora of developmental functions and adaptive growth in plants.","lang":"eng"}],"_id":"7697","date_published":"2020-09-01T00:00:00Z","file":[{"file_name":"2020_09_NewPhytologist_Zhang.pdf","creator":"dernst","file_size":3643395,"checksum":"8e8150dbbba8cb65b72f81d1f0864b8b","date_updated":"2020-11-24T12:19:38Z","access_level":"open_access","content_type":"application/pdf","relation":"main_file","file_id":"8799","date_created":"2020-11-24T12:19:38Z","success":1}],"oa":1,"publication_status":"published","volume":227,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"file_date_updated":"2020-11-24T12:19:38Z","title":"Directional auxin fluxes in plants by intramolecular domain‐domain co‐evolution of PIN auxin transporters","ec_funded":1,"citation":{"chicago":"Zhang, Yuzhou, Corinna Hartinger, Xiaojuan Wang, and Jiří Friml. “Directional Auxin Fluxes in Plants by Intramolecular Domain‐domain Co‐evolution of PIN Auxin Transporters.” <i>New Phytologist</i>. Wiley, 2020. <a href=\"https://doi.org/10.1111/nph.16629\">https://doi.org/10.1111/nph.16629</a>.","ieee":"Y. Zhang, C. Hartinger, X. Wang, and J. Friml, “Directional auxin fluxes in plants by intramolecular domain‐domain co‐evolution of PIN auxin transporters,” <i>New Phytologist</i>, vol. 227, no. 5. Wiley, pp. 1406–1416, 2020.","ista":"Zhang Y, Hartinger C, Wang X, Friml J. 2020. Directional auxin fluxes in plants by intramolecular domain‐domain co‐evolution of PIN auxin transporters. New Phytologist. 227(5), 1406–1416.","mla":"Zhang, Yuzhou, et al. “Directional Auxin Fluxes in Plants by Intramolecular Domain‐domain Co‐evolution of PIN Auxin Transporters.” <i>New Phytologist</i>, vol. 227, no. 5, Wiley, 2020, pp. 1406–16, doi:<a href=\"https://doi.org/10.1111/nph.16629\">10.1111/nph.16629</a>.","short":"Y. Zhang, C. Hartinger, X. Wang, J. Friml, New Phytologist 227 (2020) 1406–1416.","ama":"Zhang Y, Hartinger C, Wang X, Friml J. Directional auxin fluxes in plants by intramolecular domain‐domain co‐evolution of PIN auxin transporters. <i>New Phytologist</i>. 2020;227(5):1406-1416. doi:<a href=\"https://doi.org/10.1111/nph.16629\">10.1111/nph.16629</a>","apa":"Zhang, Y., Hartinger, C., Wang, X., &#38; Friml, J. (2020). Directional auxin fluxes in plants by intramolecular domain‐domain co‐evolution of PIN auxin transporters. <i>New Phytologist</i>. Wiley. <a href=\"https://doi.org/10.1111/nph.16629\">https://doi.org/10.1111/nph.16629</a>"},"author":[{"id":"3B6137F2-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2627-6956","last_name":"Zhang","full_name":"Zhang, Yuzhou","first_name":"Yuzhou"},{"id":"AEFB2266-8ABF-11EA-AA39-812C3623CBE4","orcid":"0000-0003-1618-2737","last_name":"Hartinger","full_name":"Hartinger, Corinna","first_name":"Corinna"},{"last_name":"Wang","full_name":"Wang, Xiaojuan","first_name":"Xiaojuan"},{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jiří","first_name":"Jiří"}],"type":"journal_article","day":"01","pmid":1,"project":[{"grant_number":"742985","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","_id":"261099A6-B435-11E9-9278-68D0E5697425","call_identifier":"H2020"},{"name":"Molecular mechanisms of endocytic cargo recognition in plants","grant_number":"I03630","call_identifier":"FWF","_id":"26538374-B435-11E9-9278-68D0E5697425"},{"name":"International IST Postdoc Fellowship Programme","grant_number":"291734","call_identifier":"FP7","_id":"25681D80-B435-11E9-9278-68D0E5697425"}],"doi":"10.1111/nph.16629","ddc":["580"],"language":[{"iso":"eng"}],"page":"1406-1416","date_created":"2020-04-30T08:43:29Z","month":"09","isi":1,"publisher":"Wiley","intvolume":"       227","status":"public","department":[{"_id":"JiFr"}],"quality_controlled":"1","publication":"New Phytologist"},{"author":[{"id":"1AE1EA24-02D0-11E9-9BAA-DAF4881429F2","orcid":"0000-0003-0619-7783","first_name":"Matous","full_name":"Glanc, Matous","last_name":"Glanc"},{"first_name":"Matyas","full_name":"Fendrych, Matyas","last_name":"Fendrych","orcid":"0000-0002-9767-8699","id":"43905548-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Friml, Jiří","first_name":"Jiří","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596"}],"type":"journal_article","day":"07","title":"PIN2 polarity establishment in arabidopsis in the absence of an intact cytoskeleton","ec_funded":1,"citation":{"apa":"Glanc, M., Fendrych, M., &#38; Friml, J. (2019). PIN2 polarity establishment in arabidopsis in the absence of an intact cytoskeleton. <i>Biomolecules</i>. MDPI. <a href=\"https://doi.org/10.3390/biom9060222\">https://doi.org/10.3390/biom9060222</a>","ama":"Glanc M, Fendrych M, Friml J. PIN2 polarity establishment in arabidopsis in the absence of an intact cytoskeleton. <i>Biomolecules</i>. 2019;9(6). doi:<a href=\"https://doi.org/10.3390/biom9060222\">10.3390/biom9060222</a>","short":"M. Glanc, M. Fendrych, J. Friml, Biomolecules 9 (2019).","mla":"Glanc, Matous, et al. “PIN2 Polarity Establishment in Arabidopsis in the Absence of an Intact Cytoskeleton.” <i>Biomolecules</i>, vol. 9, no. 6, 222, MDPI, 2019, doi:<a href=\"https://doi.org/10.3390/biom9060222\">10.3390/biom9060222</a>.","ieee":"M. Glanc, M. Fendrych, and J. Friml, “PIN2 polarity establishment in arabidopsis in the absence of an intact cytoskeleton,” <i>Biomolecules</i>, vol. 9, no. 6. MDPI, 2019.","ista":"Glanc M, Fendrych M, Friml J. 2019. PIN2 polarity establishment in arabidopsis in the absence of an intact cytoskeleton. Biomolecules. 9(6), 222.","chicago":"Glanc, Matous, Matyas Fendrych, and Jiří Friml. “PIN2 Polarity Establishment in Arabidopsis in the Absence of an Intact Cytoskeleton.” <i>Biomolecules</i>. MDPI, 2019. <a href=\"https://doi.org/10.3390/biom9060222\">https://doi.org/10.3390/biom9060222</a>."},"doi":"10.3390/biom9060222","ddc":["580"],"language":[{"iso":"eng"}],"pmid":1,"project":[{"call_identifier":"H2020","_id":"261099A6-B435-11E9-9278-68D0E5697425","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","grant_number":"742985"}],"date_created":"2019-07-07T21:59:21Z","month":"06","intvolume":"         9","status":"public","quality_controlled":"1","department":[{"_id":"JiFr"}],"publication":"Biomolecules","isi":1,"publisher":"MDPI","oa_version":"Published Version","has_accepted_license":"1","year":"2019","acknowledged_ssus":[{"_id":"Bio"}],"date_updated":"2023-08-28T12:30:24Z","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","external_id":{"isi":["000475301500018"],"pmid":["31181636"]},"scopus_import":"1","_id":"6611","date_published":"2019-06-07T00:00:00Z","abstract":[{"lang":"eng","text":"Cell polarity is crucial for the coordinated development of all multicellular organisms. In plants, this is exemplified by the PIN-FORMED (PIN) efflux carriers of the phytohormone auxin: The polar subcellular localization of the PINs is instructive to the directional intercellular auxin transport, and thus to a plethora of auxin-regulated growth and developmental processes. Despite its importance, the regulation of PIN polar subcellular localization remains poorly understood. Here, we have employed advanced live-cell imaging techniques to study the roles of microtubules and actin microfilaments in the establishment of apical polar localization of PIN2 in the epidermis of the Arabidopsis root meristem. We report that apical PIN2 polarity requires neither intact actin microfilaments nor microtubules, suggesting that the primary spatial cue for polar PIN distribution is likely independent of cytoskeleton-guided endomembrane trafficking."}],"file":[{"date_created":"2019-07-08T15:46:32Z","content_type":"application/pdf","file_id":"6625","relation":"main_file","checksum":"1ce1bd36038fe5381057a1bcc6760083","date_updated":"2020-07-14T12:47:34Z","access_level":"open_access","file_name":"biomolecules-2019-Matous.pdf","file_size":1066773,"creator":"kschuh"}],"article_number":"222","issue":"6","article_processing_charge":"No","volume":9,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"file_date_updated":"2020-07-14T12:47:34Z","oa":1,"publication_status":"published"},{"date_created":"2019-07-11T12:00:32Z","month":"07","isi":1,"publisher":"MDPI","status":"public","intvolume":"        20","department":[{"_id":"JiFr"}],"quality_controlled":"1","publication":"International Journal of Molecular Sciences","title":"Reorientation of cortical microtubule arrays in the hypocotyl of arabidopsis thaliana is induced by the cell growth process and independent of auxin signaling","ec_funded":1,"citation":{"short":"M. Adamowski, L. Li, J. Friml, International Journal of Molecular Sciences 20 (2019).","apa":"Adamowski, M., Li, L., &#38; Friml, J. (2019). Reorientation of cortical microtubule arrays in the hypocotyl of arabidopsis thaliana is induced by the cell growth process and independent of auxin signaling. <i>International Journal of Molecular Sciences</i>. MDPI. <a href=\"https://doi.org/10.3390/ijms20133337\">https://doi.org/10.3390/ijms20133337</a>","ama":"Adamowski M, Li L, Friml J. Reorientation of cortical microtubule arrays in the hypocotyl of arabidopsis thaliana is induced by the cell growth process and independent of auxin signaling. <i>International Journal of Molecular Sciences</i>. 2019;20(13). doi:<a href=\"https://doi.org/10.3390/ijms20133337\">10.3390/ijms20133337</a>","ista":"Adamowski M, Li L, Friml J. 2019. Reorientation of cortical microtubule arrays in the hypocotyl of arabidopsis thaliana is induced by the cell growth process and independent of auxin signaling. International Journal of Molecular Sciences. 20(13), 3337.","ieee":"M. Adamowski, L. Li, and J. Friml, “Reorientation of cortical microtubule arrays in the hypocotyl of arabidopsis thaliana is induced by the cell growth process and independent of auxin signaling,” <i>International Journal of Molecular Sciences</i>, vol. 20, no. 13. MDPI, 2019.","chicago":"Adamowski, Maciek, Lanxin Li, and Jiří Friml. “Reorientation of Cortical Microtubule Arrays in the Hypocotyl of Arabidopsis Thaliana Is Induced by the Cell Growth Process and Independent of Auxin Signaling.” <i>International Journal of Molecular Sciences</i>. MDPI, 2019. <a href=\"https://doi.org/10.3390/ijms20133337\">https://doi.org/10.3390/ijms20133337</a>.","mla":"Adamowski, Maciek, et al. “Reorientation of Cortical Microtubule Arrays in the Hypocotyl of Arabidopsis Thaliana Is Induced by the Cell Growth Process and Independent of Auxin Signaling.” <i>International Journal of Molecular Sciences</i>, vol. 20, no. 13, 3337, MDPI, 2019, doi:<a href=\"https://doi.org/10.3390/ijms20133337\">10.3390/ijms20133337</a>."},"type":"journal_article","author":[{"last_name":"Adamowski","full_name":"Adamowski, Maciek","first_name":"Maciek","id":"45F536D2-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6463-5257"},{"orcid":"0000-0002-5607-272X","id":"367EF8FA-F248-11E8-B48F-1D18A9856A87","last_name":"Li","full_name":"Li, Lanxin","first_name":"Lanxin"},{"last_name":"Friml","first_name":"Jiří","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596"}],"day":"07","project":[{"call_identifier":"FP7","_id":"25716A02-B435-11E9-9278-68D0E5697425","name":"Polarity and subcellular dynamics in plants","grant_number":"282300"},{"call_identifier":"H2020","_id":"2564DBCA-B435-11E9-9278-68D0E5697425","grant_number":"665385","name":"International IST Doctoral Program"},{"_id":"B67AFEDC-15C9-11EA-A837-991A96BB2854","name":"IST Austria Open Access Fund"}],"pmid":1,"related_material":{"record":[{"relation":"dissertation_contains","id":"10083","status":"public"}]},"ddc":["580"],"doi":"10.3390/ijms20133337","language":[{"iso":"eng"}],"issue":"13","article_processing_charge":"Yes","date_published":"2019-07-07T00:00:00Z","_id":"6627","abstract":[{"lang":"eng","text":"Cortical microtubule arrays in elongating epidermal cells in both the root and stem of plants have the propensity of dynamic reorientations that are correlated with the activation or inhibition of growth. Factors regulating plant growth, among them the hormone auxin, have been recognized as regulators of microtubule array orientations. Some previous work in the field has aimed at elucidating the causal relationship between cell growth, the signaling of auxin or other growth-regulating factors, and microtubule array reorientations, with various conclusions. Here, we revisit this problem of causality with a comprehensive set of experiments in Arabidopsis thaliana, using the now available pharmacological and genetic tools. We use isolated, auxin-depleted hypocotyls, an experimental system allowing for full control of both growth and auxin signaling. We demonstrate that reorientation of microtubules is not directly triggered by an auxin signal during growth activation. Instead, reorientation is triggered by the activation of the growth process itself and is auxin-independent in its nature. We discuss these findings in the context of previous relevant work, including that on the mechanical regulation of microtubule array orientation."}],"article_number":"3337","file":[{"date_created":"2019-07-17T06:17:15Z","relation":"main_file","file_id":"6645","content_type":"application/pdf","checksum":"dd9d1cbb933a72ceb666c9667890ac51","access_level":"open_access","date_updated":"2020-07-14T12:47:34Z","file_size":3330291,"creator":"dernst","file_name":"2019_JournalMolecularScience_Adamowski.pdf"}],"oa":1,"publication_status":"published","volume":20,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"file_date_updated":"2020-07-14T12:47:34Z","year":"2019","has_accepted_license":"1","oa_version":"Published Version","article_type":"original","publication_identifier":{"eissn":["1422-0067"]},"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","date_updated":"2025-05-07T11:12:33Z","scopus_import":"1","external_id":{"isi":["000477041100221"],"pmid":["31284661"]}},{"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","date_updated":"2023-08-29T07:02:44Z","scopus_import":"1","external_id":{"pmid":["31375675"],"isi":["000478576500012"]},"publication_identifier":{"issn":["2041-1723"]},"article_type":"original","oa_version":"Published Version","year":"2019","has_accepted_license":"1","publication_status":"published","oa":1,"file_date_updated":"2020-07-14T12:47:40Z","volume":10,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"article_processing_charge":"No","article_number":"3480","file":[{"date_created":"2019-08-12T07:09:20Z","file_id":"6798","relation":"main_file","content_type":"application/pdf","access_level":"open_access","date_updated":"2020-07-14T12:47:40Z","checksum":"d2c654fdb97f33078f606fe0c298bf6e","creator":"dernst","file_size":6406141,"file_name":"2019_NatureComm_Zhang.pdf"}],"date_published":"2019-08-02T00:00:00Z","_id":"6778","abstract":[{"lang":"eng","text":"An important adaptation during colonization of land by plants is gravitropic growth of roots, which enabled roots to reach water and nutrients, and firmly anchor plants in the ground. Here we provide insights into the evolution of an efficient root gravitropic mechanism in the seed plants. Architectural innovation, with gravity perception constrained in the root tips\r\nalong with a shootward transport route for the phytohormone auxin, appeared only upon the emergence of seed plants. Interspecies complementation and protein domain swapping revealed functional innovations within the PIN family of auxin transporters leading to the evolution of gravitropism-specific PINs. The unique apical/shootward subcellular localization of PIN proteins is the major evolutionary innovation that connected the anatomically separated sites of gravity perception and growth response via the mobile auxin signal. We conclude that the crucial anatomical and functional components emerged hand-in-hand to facilitate the evolution of fast gravitropic response, which is one of the major adaptations of seed plants to dry land."}],"related_material":{"link":[{"description":"News on IST Homepage","url":"https://ist.ac.at/en/news/when-plant-roots-learned-to-follow-gravity/","relation":"press_release"}]},"pmid":1,"project":[{"grant_number":"742985","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","_id":"261099A6-B435-11E9-9278-68D0E5697425","call_identifier":"H2020"},{"grant_number":"I03630","name":"Molecular mechanisms of endocytic cargo recognition in plants","_id":"26538374-B435-11E9-9278-68D0E5697425","call_identifier":"FWF"},{"grant_number":"291734","name":"International IST Postdoc Fellowship Programme","_id":"25681D80-B435-11E9-9278-68D0E5697425","call_identifier":"FP7"}],"language":[{"iso":"eng"}],"ddc":["580"],"doi":"10.1038/s41467-019-11471-8","ec_funded":1,"citation":{"apa":"Zhang, Y., Xiao, G., Wang, X., Zhang, X., &#38; Friml, J. (2019). Evolution of fast root gravitropism in seed plants. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-019-11471-8\">https://doi.org/10.1038/s41467-019-11471-8</a>","ama":"Zhang Y, Xiao G, Wang X, Zhang X, Friml J. Evolution of fast root gravitropism in seed plants. <i>Nature Communications</i>. 2019;10. doi:<a href=\"https://doi.org/10.1038/s41467-019-11471-8\">10.1038/s41467-019-11471-8</a>","short":"Y. Zhang, G. Xiao, X. Wang, X. Zhang, J. Friml, Nature Communications 10 (2019).","mla":"Zhang, Yuzhou, et al. “Evolution of Fast Root Gravitropism in Seed Plants.” <i>Nature Communications</i>, vol. 10, 3480, Springer Nature, 2019, doi:<a href=\"https://doi.org/10.1038/s41467-019-11471-8\">10.1038/s41467-019-11471-8</a>.","ieee":"Y. Zhang, G. Xiao, X. Wang, X. Zhang, and J. Friml, “Evolution of fast root gravitropism in seed plants,” <i>Nature Communications</i>, vol. 10. Springer Nature, 2019.","ista":"Zhang Y, Xiao G, Wang X, Zhang X, Friml J. 2019. Evolution of fast root gravitropism in seed plants. Nature Communications. 10, 3480.","chicago":"Zhang, Yuzhou, G Xiao, X Wang, Xixi Zhang, and Jiří Friml. “Evolution of Fast Root Gravitropism in Seed Plants.” <i>Nature Communications</i>. Springer Nature, 2019. <a href=\"https://doi.org/10.1038/s41467-019-11471-8\">https://doi.org/10.1038/s41467-019-11471-8</a>."},"title":"Evolution of fast root gravitropism in seed plants","day":"02","type":"journal_article","author":[{"full_name":"Zhang, Yuzhou","first_name":"Yuzhou","last_name":"Zhang","id":"3B6137F2-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2627-6956"},{"first_name":"G","full_name":"Xiao, G","last_name":"Xiao"},{"full_name":"Wang, X","first_name":"X","last_name":"Wang"},{"id":"61A66458-47E9-11EA-85BA-8AEAAF14E49A","orcid":"0000-0001-7048-4627","last_name":"Zhang","full_name":"Zhang, Xixi","first_name":"Xixi"},{"full_name":"Friml, Jiří","first_name":"Jiří","last_name":"Friml","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"publisher":"Springer Nature","isi":1,"department":[{"_id":"JiFr"}],"quality_controlled":"1","publication":"Nature Communications","intvolume":"        10","status":"public","month":"08","date_created":"2019-08-09T08:46:26Z"},{"file_date_updated":"2020-07-14T12:47:45Z","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"volume":52,"publication_status":"published","oa":1,"file":[{"access_level":"open_access","date_updated":"2020-07-14T12:47:45Z","checksum":"d6fd68a6e965f1efe3f0bf2d2070a616","creator":"dernst","file_size":1659288,"file_name":"2019_CurrentOpinionPlant_Hoermayer.pdf","date_created":"2019-10-14T14:48:21Z","file_id":"6946","relation":"main_file","content_type":"application/pdf"}],"abstract":[{"text":"Plants as sessile organisms are constantly under attack by herbivores, rough environmental situations, or mechanical pressure. These challenges often lead to the induction of wounds or destruction of already specified and developed tissues. Additionally, wounding makes plants vulnerable to invasion by pathogens, which is why wound signalling often triggers specific defence responses. To stay competitive or, eventually, survive under these circumstances, plants need to regenerate efficiently, which in rigid, tissue migration-incompatible plant tissues requires post-embryonic patterning and organogenesis. Now, several studies used laser-assisted single cell ablation in the Arabidopsis root tip as a minimal wounding proxy. Here, we discuss their findings and put them into context of a broader spectrum of wound signalling, pathogen responses and tissue as well as organ regeneration.","lang":"eng"}],"_id":"6943","date_published":"2019-12-01T00:00:00Z","article_processing_charge":"No","external_id":{"pmid":["31585333"],"isi":["000502890600017"]},"scopus_import":"1","date_updated":"2024-03-25T23:30:06Z","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","publication_identifier":{"issn":["1369-5266"]},"article_type":"original","has_accepted_license":"1","oa_version":"Published Version","year":"2019","publication":"Current Opinion in Plant Biology","quality_controlled":"1","department":[{"_id":"JiFr"}],"status":"public","intvolume":"        52","publisher":"Elsevier","isi":1,"month":"12","date_created":"2019-10-14T07:00:24Z","page":"124-130","language":[{"iso":"eng"}],"ddc":["580"],"doi":"10.1016/j.pbi.2019.08.006","project":[{"grant_number":"742985","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","call_identifier":"H2020","_id":"261099A6-B435-11E9-9278-68D0E5697425"}],"pmid":1,"related_material":{"record":[{"id":"9992","relation":"dissertation_contains","status":"public"}]},"day":"01","author":[{"orcid":"0000-0001-8295-2926","id":"2EEE7A2A-F248-11E8-B48F-1D18A9856A87","full_name":"Hörmayer, Lukas","first_name":"Lukas","last_name":"Hörmayer"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","first_name":"Jiří","full_name":"Friml, Jiří","last_name":"Friml"}],"type":"journal_article","citation":{"ieee":"L. Hörmayer and J. Friml, “Targeted cell ablation-based insights into wound healing and restorative patterning,” <i>Current Opinion in Plant Biology</i>, vol. 52. Elsevier, pp. 124–130, 2019.","ista":"Hörmayer L, Friml J. 2019. Targeted cell ablation-based insights into wound healing and restorative patterning. Current Opinion in Plant Biology. 52, 124–130.","chicago":"Hörmayer, Lukas, and Jiří Friml. “Targeted Cell Ablation-Based Insights into Wound Healing and Restorative Patterning.” <i>Current Opinion in Plant Biology</i>. Elsevier, 2019. <a href=\"https://doi.org/10.1016/j.pbi.2019.08.006\">https://doi.org/10.1016/j.pbi.2019.08.006</a>.","mla":"Hörmayer, Lukas, and Jiří Friml. “Targeted Cell Ablation-Based Insights into Wound Healing and Restorative Patterning.” <i>Current Opinion in Plant Biology</i>, vol. 52, Elsevier, 2019, pp. 124–30, doi:<a href=\"https://doi.org/10.1016/j.pbi.2019.08.006\">10.1016/j.pbi.2019.08.006</a>.","short":"L. Hörmayer, J. Friml, Current Opinion in Plant Biology 52 (2019) 124–130.","apa":"Hörmayer, L., &#38; Friml, J. (2019). Targeted cell ablation-based insights into wound healing and restorative patterning. <i>Current Opinion in Plant Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.pbi.2019.08.006\">https://doi.org/10.1016/j.pbi.2019.08.006</a>","ama":"Hörmayer L, Friml J. Targeted cell ablation-based insights into wound healing and restorative patterning. <i>Current Opinion in Plant Biology</i>. 2019;52:124-130. doi:<a href=\"https://doi.org/10.1016/j.pbi.2019.08.006\">10.1016/j.pbi.2019.08.006</a>"},"ec_funded":1,"title":"Targeted cell ablation-based insights into wound healing and restorative patterning"},{"has_accepted_license":"1","oa_version":"Published Version","year":"2019","article_type":"original","publication_identifier":{"eissn":["1091-6490"],"issn":["0027-8424"]},"date_updated":"2023-10-17T12:32:37Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","scopus_import":"1","external_id":{"isi":["000490183000068"],"pmid":["31575745"]},"issue":"42","article_processing_charge":"No","_id":"6999","date_published":"2019-10-15T00:00:00Z","abstract":[{"lang":"eng","text":"Plasmodesmata (PD) are plant-specific membrane-lined channels that create cytoplasmic and membrane continuities between adjacent cells, thereby facilitating cell–cell communication and virus movement. Plant cells have evolved diverse mechanisms to regulate PD plasticity in response to numerous environmental stimuli. In particular, during defense against plant pathogens, the defense hormone, salicylic acid (SA), plays a crucial role in the regulation of PD permeability in a callose-dependent manner. Here, we uncover a mechanism by which plants restrict the spreading of virus and PD cargoes using SA signaling by increasing lipid order and closure of PD. We showed that exogenous SA application triggered the compartmentalization of lipid raft nanodomains through a modulation of the lipid raft-regulatory protein, Remorin (REM). Genetic studies, superresolution imaging, and transmission electron microscopy observation together demonstrated that Arabidopsis REM1.2 and REM1.3 are crucial for plasma membrane nanodomain assembly to control PD aperture and functionality. In addition, we also found that a 14-3-3 epsilon protein modulates REM clustering and membrane nanodomain compartmentalization through its direct interaction with REM proteins. This study unveils a molecular mechanism by which the key plant defense hormone, SA, triggers membrane lipid nanodomain reorganization, thereby regulating PD closure to impede virus spreading."}],"file":[{"file_name":"2019_PNAS_Huang.pdf","creator":"dernst","file_size":3287466,"date_updated":"2020-07-14T12:47:46Z","access_level":"open_access","checksum":"258c666bc6253eab81961f61169eefae","content_type":"application/pdf","relation":"main_file","file_id":"7012","date_created":"2019-11-13T08:22:28Z"}],"oa":1,"publication_status":"published","volume":116,"tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","image":"/images/cc_by_nc_nd.png","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)"},"file_date_updated":"2020-07-14T12:47:46Z","title":"Salicylic acid-mediated plasmodesmal closure via Remorin-dependent lipid organization","citation":{"mla":"Huang, D., et al. “Salicylic Acid-Mediated Plasmodesmal Closure via Remorin-Dependent Lipid Organization.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 116, no. 42, Proceedings of the National Academy of Sciences, 2019, pp. 21274–84, doi:<a href=\"https://doi.org/10.1073/pnas.1911892116\">10.1073/pnas.1911892116</a>.","ista":"Huang D, Sun Y, Ma Z, Ke M, Cui Y, Chen Z, Chen C, Ji C, Tran T, Yang L, Lam S, Han Y, Shu G, Friml J, Miao Y, Jiang L, Chen X. 2019. Salicylic acid-mediated plasmodesmal closure via Remorin-dependent lipid organization. Proceedings of the National Academy of Sciences of the United States of America. 116(42), 21274–21284.","ieee":"D. Huang <i>et al.</i>, “Salicylic acid-mediated plasmodesmal closure via Remorin-dependent lipid organization,” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 116, no. 42. Proceedings of the National Academy of Sciences, pp. 21274–21284, 2019.","chicago":"Huang, D, Y Sun, Z Ma, M Ke, Y Cui, Z Chen, C Chen, et al. “Salicylic Acid-Mediated Plasmodesmal Closure via Remorin-Dependent Lipid Organization.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>. Proceedings of the National Academy of Sciences, 2019. <a href=\"https://doi.org/10.1073/pnas.1911892116\">https://doi.org/10.1073/pnas.1911892116</a>.","apa":"Huang, D., Sun, Y., Ma, Z., Ke, M., Cui, Y., Chen, Z., … Chen, X. (2019). Salicylic acid-mediated plasmodesmal closure via Remorin-dependent lipid organization. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. Proceedings of the National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.1911892116\">https://doi.org/10.1073/pnas.1911892116</a>","ama":"Huang D, Sun Y, Ma Z, et al. Salicylic acid-mediated plasmodesmal closure via Remorin-dependent lipid organization. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. 2019;116(42):21274-21284. doi:<a href=\"https://doi.org/10.1073/pnas.1911892116\">10.1073/pnas.1911892116</a>","short":"D. Huang, Y. Sun, Z. Ma, M. Ke, Y. Cui, Z. Chen, C. Chen, C. Ji, T. Tran, L. Yang, S. Lam, Y. Han, G. Shu, J. Friml, Y. Miao, L. Jiang, X. Chen, Proceedings of the National Academy of Sciences of the United States of America 116 (2019) 21274–21284."},"author":[{"last_name":"Huang","first_name":"D","full_name":"Huang, D"},{"last_name":"Sun","first_name":"Y","full_name":"Sun, Y"},{"first_name":"Z","full_name":"Ma, Z","last_name":"Ma"},{"last_name":"Ke","full_name":"Ke, M","first_name":"M"},{"last_name":"Cui","full_name":"Cui, Y","first_name":"Y"},{"full_name":"Chen, Z","first_name":"Z","last_name":"Chen"},{"last_name":"Chen","first_name":"C","full_name":"Chen, C"},{"first_name":"C","full_name":"Ji, C","last_name":"Ji"},{"last_name":"Tran","first_name":"TM","full_name":"Tran, TM"},{"last_name":"Yang","full_name":"Yang, L","first_name":"L"},{"last_name":"Lam","full_name":"Lam, SM","first_name":"SM"},{"first_name":"Y","full_name":"Han, Y","last_name":"Han"},{"last_name":"Shu","full_name":"Shu, G","first_name":"G"},{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","first_name":"Jiří","last_name":"Friml"},{"full_name":"Miao, Y","first_name":"Y","last_name":"Miao"},{"last_name":"Jiang","first_name":"L","full_name":"Jiang, L"},{"last_name":"Chen","first_name":"X","full_name":"Chen, X"}],"type":"journal_article","day":"15","related_material":{"link":[{"relation":"erratum","url":"https://doi.org/10.1073/pnas.2004738117"}]},"pmid":1,"ddc":["580"],"doi":"10.1073/pnas.1911892116","language":[{"iso":"eng"}],"page":"21274-21284","date_created":"2019-11-12T11:42:05Z","month":"10","isi":1,"publisher":"Proceedings of the National Academy of Sciences","intvolume":"       116","status":"public","department":[{"_id":"JiFr"}],"quality_controlled":"1","publication":"Proceedings of the National Academy of Sciences of the United States of America"},{"publication_status":"published","oa":1,"file_date_updated":"2020-10-14T08:54:49Z","volume":5,"article_processing_charge":"No","issue":"11","file":[{"content_type":"application/pdf","relation":"main_file","file_id":"8660","success":1,"date_created":"2020-10-14T08:54:49Z","file_name":"2019_NaturePlants_Skokan_accepted.pdf","creator":"dernst","file_size":1980851,"date_updated":"2020-10-14T08:54:49Z","access_level":"open_access","checksum":"94e0426856aad9a9bd0135d5436efbf1"}],"_id":"7106","date_published":"2019-11-01T00:00:00Z","abstract":[{"text":"PIN-FORMED (PIN) transporters mediate directional, intercellular movement of the phytohormone auxin in land plants. To elucidate the evolutionary origins of this developmentally crucial mechanism, we analysed the single PIN homologue of a simple green alga Klebsormidium flaccidum. KfPIN functions as a plasma membrane-localized auxin exporter in land plants and heterologous models. While its role in algae remains unclear, PIN-driven auxin export is probably an ancient and conserved trait within streptophytes.","lang":"eng"}],"external_id":{"isi":["000496526100010"],"pmid":["31712756"]},"scopus_import":"1","date_updated":"2023-09-06T11:09:49Z","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","publication_identifier":{"issn":["2055-0278"]},"article_type":"original","has_accepted_license":"1","year":"2019","oa_version":"Submitted Version","publisher":"Springer Nature","isi":1,"publication":"Nature Plants","department":[{"_id":"JiFr"}],"quality_controlled":"1","intvolume":"         5","status":"public","page":"1114-1119","month":"11","date_created":"2019-11-25T09:08:04Z","pmid":1,"project":[{"name":"Tracing Evolution of Auxin Transport and Polarity in Plants","grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425","call_identifier":"H2020"}],"language":[{"iso":"eng"}],"doi":"10.1038/s41477-019-0542-5","ddc":["580"],"citation":{"short":"R. Skokan, E. Medvecká, T. Viaene, S. Vosolsobě, M. Zwiewka, K. Müller, P. Skůpa, M. Karady, Y. Zhang, D.P. Janacek, U.Z. Hammes, K. Ljung, T. Nodzyński, J. Petrášek, J. Friml, Nature Plants 5 (2019) 1114–1119.","apa":"Skokan, R., Medvecká, E., Viaene, T., Vosolsobě, S., Zwiewka, M., Müller, K., … Friml, J. (2019). PIN-driven auxin transport emerged early in streptophyte evolution. <i>Nature Plants</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41477-019-0542-5\">https://doi.org/10.1038/s41477-019-0542-5</a>","ama":"Skokan R, Medvecká E, Viaene T, et al. PIN-driven auxin transport emerged early in streptophyte evolution. <i>Nature Plants</i>. 2019;5(11):1114-1119. doi:<a href=\"https://doi.org/10.1038/s41477-019-0542-5\">10.1038/s41477-019-0542-5</a>","ista":"Skokan R, Medvecká E, Viaene T, Vosolsobě S, Zwiewka M, Müller K, Skůpa P, Karady M, Zhang Y, Janacek DP, Hammes UZ, Ljung K, Nodzyński T, Petrášek J, Friml J. 2019. PIN-driven auxin transport emerged early in streptophyte evolution. Nature Plants. 5(11), 1114–1119.","ieee":"R. Skokan <i>et al.</i>, “PIN-driven auxin transport emerged early in streptophyte evolution,” <i>Nature Plants</i>, vol. 5, no. 11. Springer Nature, pp. 1114–1119, 2019.","chicago":"Skokan, Roman, Eva Medvecká, Tom Viaene, Stanislav Vosolsobě, Marta Zwiewka, Karel Müller, Petr Skůpa, et al. “PIN-Driven Auxin Transport Emerged Early in Streptophyte Evolution.” <i>Nature Plants</i>. Springer Nature, 2019. <a href=\"https://doi.org/10.1038/s41477-019-0542-5\">https://doi.org/10.1038/s41477-019-0542-5</a>.","mla":"Skokan, Roman, et al. “PIN-Driven Auxin Transport Emerged Early in Streptophyte Evolution.” <i>Nature Plants</i>, vol. 5, no. 11, Springer Nature, 2019, pp. 1114–19, doi:<a href=\"https://doi.org/10.1038/s41477-019-0542-5\">10.1038/s41477-019-0542-5</a>."},"ec_funded":1,"title":"PIN-driven auxin transport emerged early in streptophyte evolution","day":"01","author":[{"last_name":"Skokan","first_name":"Roman","full_name":"Skokan, Roman"},{"last_name":"Medvecká","first_name":"Eva","full_name":"Medvecká, Eva"},{"first_name":"Tom","full_name":"Viaene, Tom","last_name":"Viaene"},{"full_name":"Vosolsobě, Stanislav","first_name":"Stanislav","last_name":"Vosolsobě"},{"full_name":"Zwiewka, Marta","first_name":"Marta","last_name":"Zwiewka"},{"last_name":"Müller","full_name":"Müller, Karel","first_name":"Karel"},{"full_name":"Skůpa, Petr","first_name":"Petr","last_name":"Skůpa"},{"last_name":"Karady","first_name":"Michal","full_name":"Karady, Michal"},{"last_name":"Zhang","full_name":"Zhang, Yuzhou","first_name":"Yuzhou"},{"first_name":"Dorina P.","full_name":"Janacek, Dorina P.","last_name":"Janacek"},{"full_name":"Hammes, Ulrich Z.","first_name":"Ulrich Z.","last_name":"Hammes"},{"first_name":"Karin","full_name":"Ljung, Karin","last_name":"Ljung"},{"last_name":"Nodzyński","first_name":"Tomasz","full_name":"Nodzyński, Tomasz"},{"last_name":"Petrášek","first_name":"Jan","full_name":"Petrášek, Jan"},{"first_name":"Jiří","full_name":"Friml, Jiří","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596"}],"type":"journal_article"},{"doi":"10.1038/s41422-019-0254-4","language":[{"iso":"eng"}],"pmid":1,"type":"journal_article","author":[{"first_name":"Scott A","full_name":"Sinclair, Scott A","last_name":"Sinclair","orcid":"0000-0002-4566-0593","id":"2D99FE6A-F248-11E8-B48F-1D18A9856A87"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","last_name":"Friml","full_name":"Friml, Jiří","first_name":"Jiří"}],"day":"01","title":"Defying gravity: a plant's quest for moisture","citation":{"ama":"Sinclair SA, Friml J. Defying gravity: a plant’s quest for moisture. <i>Cell Research</i>. 2019;29:965-966. doi:<a href=\"https://doi.org/10.1038/s41422-019-0254-4\">10.1038/s41422-019-0254-4</a>","apa":"Sinclair, S. A., &#38; Friml, J. (2019). Defying gravity: a plant’s quest for moisture. <i>Cell Research</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41422-019-0254-4\">https://doi.org/10.1038/s41422-019-0254-4</a>","short":"S.A. Sinclair, J. Friml, Cell Research 29 (2019) 965–966.","mla":"Sinclair, Scott A., and Jiří Friml. “Defying Gravity: A Plant’s Quest for Moisture.” <i>Cell Research</i>, vol. 29, Springer Nature, 2019, pp. 965–66, doi:<a href=\"https://doi.org/10.1038/s41422-019-0254-4\">10.1038/s41422-019-0254-4</a>.","chicago":"Sinclair, Scott A, and Jiří Friml. “Defying Gravity: A Plant’s Quest for Moisture.” <i>Cell Research</i>. Springer Nature, 2019. <a href=\"https://doi.org/10.1038/s41422-019-0254-4\">https://doi.org/10.1038/s41422-019-0254-4</a>.","ista":"Sinclair SA, Friml J. 2019. Defying gravity: a plant’s quest for moisture. Cell Research. 29, 965–966.","ieee":"S. A. Sinclair and J. Friml, “Defying gravity: a plant’s quest for moisture,” <i>Cell Research</i>, vol. 29. Springer Nature, pp. 965–966, 2019."},"status":"public","intvolume":"        29","quality_controlled":"1","department":[{"_id":"JiFr"}],"publication":"Cell Research","isi":1,"publisher":"Springer Nature","date_created":"2019-12-02T12:30:48Z","month":"12","page":"965-966","publication_identifier":{"eissn":["1748-7838"],"issn":["1001-0602"]},"date_updated":"2023-09-06T11:20:58Z","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","external_id":{"isi":["000500749600001"],"pmid":["31745287"]},"scopus_import":"1","oa_version":"Published Version","year":"2019","article_type":"original","volume":29,"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1038/s41422-019-0254-4"}],"oa":1,"publication_status":"published","date_published":"2019-12-01T00:00:00Z","_id":"7143","abstract":[{"text":"Roots grow downwards parallel to the gravity vector, to anchor a plant in soil and acquire water and nutrients, using a gravitropic mechanism dependent on the asymmetric distribution of the phytohormone auxin. Recently, Chang et al. demonstrate that asymmetric distribution of another phytohormone, cytokinin, directs root growth towards higher water content.","lang":"eng"}],"article_processing_charge":"No"},{"has_accepted_license":"1","oa_version":"Published Version","year":"2019","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"}],"publication_identifier":{"eissn":["2663-337X"]},"date_updated":"2025-05-07T11:12:29Z","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","_id":"7172","abstract":[{"lang":"eng","text":"The development and growth of Arabidopsis thaliana is regulated by a combination of genetic programing and also by the environmental influences. An important role in these processes play the phytohormones and among them, auxin is crucial as it controls many important functions. It is transported through the whole plant body by creating local and temporal concentration maxima and minima, which have an impact on the cell status, tissue and organ identity. Auxin has the property to undergo a directional and finely regulated cell-to-cell transport, which is enabled by the transport proteins, localized on the plasma membrane. An important role in this process have the PIN auxin efflux proteins, which have an asymmetric/polar subcellular localization and determine the directionality of the auxin transport. During the last years, there were significant advances in understanding how the trafficking molecular machineries function, including studies on molecular interactions, function, subcellular localization and intracellular distribution. However, there is still a lack of detailed characterization on the steps of endocytosis, exocytosis, endocytic recycling and degradation. Due to this fact, I focused on the identification of novel trafficking factors and better characterization of the intracellular trafficking pathways. My PhD thesis consists of an introductory chapter, three experimental chapters, a chapter containing general discussion, conclusions and perspectives and also an appendix chapter with published collaborative papers.\r\nThe first chapter is separated in two different parts: I start by a general introduction to auxin biology and then I introduce the trafficking pathways in the model plant Arabidopsis thaliana. Then, I explain also the phosphorylation-signals for polar targeting and also the roles of the phytohormone strigolactone.\r\nThe second chapter includes the characterization of bar1/sacsin mutant, which was identified in a forward genetic screen for novel trafficking components in Arabidopsis thaliana, where by the implementation of an EMS-treated pPIN1::PIN1-GFP marker line and by using the established inhibitor of ARF-GEFs, Brefeldin A (BFA) as a tool to study trafficking processes, we identified a novel factor, which is mediating the adaptation of the plant cell to ARF-GEF inhibition. The mutation is in a previously uncharacterized gene, encoding a very big protein that we, based on its homologies, called SACSIN with domains suggesting roles as a molecular chaperon or as a component of the ubiquitin-proteasome system. Our physiology and imaging studies revealed that SACSIN is a crucial plant cell component of the adaptation to the ARF-GEF inhibition.\r\nThe third chapter includes six subchapters, where I focus on the role of the phytohormone strigolactone, which interferes with auxin feedback on PIN internalization. Strigolactone moderates the polar auxin transport by increasing the internalization of the PIN auxin efflux carriers, which reduces the canalization related growth responses. In addition, I also studied the role of phosphorylation in the strigolactone regulation of auxin feedback on PIN internalization. In this chapter I also present my results on the MAX2-dependence of strigolactone-mediated root growth inhibition and I also share my results on the auxin metabolomics profiling after application of GR24.\r\nIn the fourth chapter I studied the effect of two small molecules ES-9 and ES9-17, which were identified from a collection of small molecules with the property to impair the clathrin-mediated endocytosis.\r\nIn the fifth chapter, I discuss all my observations and experimental findings and suggest alternative hypothesis to interpret my results.\r\nIn the appendix there are three collaborative published projects. In the first, I participated in the characterization of the role of ES9 as a small molecule, which is inhibitor of clathrin- mediated endocytosis in different model organisms. In the second paper, I contributed to the characterization of another small molecule ES9-17, which is a non-protonophoric analog of ES9 and also impairs the clathrin-mediated endocytosis not only in plant cells, but also in mammalian HeLa cells. Last but not least, I also attach another paper, where I tried to establish the grafting method as a technique in our lab to study canalization related processes."}],"date_published":"2019-12-12T00:00:00Z","file":[{"checksum":"ef981c1a3b1d9da0edcbedcff4970d37","date_updated":"2020-07-14T12:47:51Z","access_level":"closed","file_name":"Thesis_Mina_final_upload_7.docx","file_size":20454014,"creator":"mvasilev","date_created":"2019-12-12T09:32:36Z","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","relation":"source_file","file_id":"7175"},{"file_name":"Thesis_Mina_final_upload_7.pdf","creator":"mvasilev","file_size":11565025,"checksum":"3882c4585e46c9cfb486e4225cad54ab","date_updated":"2020-07-14T12:47:51Z","access_level":"open_access","content_type":"application/pdf","relation":"main_file","file_id":"7176","date_created":"2019-12-12T09:33:10Z"}],"article_processing_charge":"No","file_date_updated":"2020-07-14T12:47:51Z","oa":1,"publication_status":"published","author":[{"last_name":"Vasileva","full_name":"Vasileva, Mina K","first_name":"Mina K","id":"3407EB18-F248-11E8-B48F-1D18A9856A87"}],"type":"dissertation","alternative_title":["ISTA Thesis"],"day":"12","title":"Molecular mechanisms of endomembrane trafficking in Arabidopsis thaliana","citation":{"mla":"Vasileva, Mina K. <i>Molecular Mechanisms of Endomembrane Trafficking in Arabidopsis Thaliana</i>. Institute of Science and Technology Austria, 2019, doi:<a href=\"https://doi.org/10.15479/AT:ISTA:7172\">10.15479/AT:ISTA:7172</a>.","ieee":"M. K. Vasileva, “Molecular mechanisms of endomembrane trafficking in Arabidopsis thaliana,” Institute of Science and Technology Austria, 2019.","ista":"Vasileva MK. 2019. Molecular mechanisms of endomembrane trafficking in Arabidopsis thaliana. Institute of Science and Technology Austria.","chicago":"Vasileva, Mina K. “Molecular Mechanisms of Endomembrane Trafficking in Arabidopsis Thaliana.” Institute of Science and Technology Austria, 2019. <a href=\"https://doi.org/10.15479/AT:ISTA:7172\">https://doi.org/10.15479/AT:ISTA:7172</a>.","apa":"Vasileva, M. K. (2019). <i>Molecular mechanisms of endomembrane trafficking in Arabidopsis thaliana</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT:ISTA:7172\">https://doi.org/10.15479/AT:ISTA:7172</a>","ama":"Vasileva MK. Molecular mechanisms of endomembrane trafficking in Arabidopsis thaliana. 2019. doi:<a href=\"https://doi.org/10.15479/AT:ISTA:7172\">10.15479/AT:ISTA:7172</a>","short":"M.K. Vasileva, Molecular Mechanisms of Endomembrane Trafficking in Arabidopsis Thaliana, Institute of Science and Technology Austria, 2019."},"doi":"10.15479/AT:ISTA:7172","ddc":["570"],"language":[{"iso":"eng"}],"related_material":{"record":[{"status":"public","relation":"part_of_dissertation","id":"6377"},{"relation":"part_of_dissertation","id":"449","status":"public"},{"status":"public","relation":"part_of_dissertation","id":"1346"}]},"supervisor":[{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","last_name":"Friml","first_name":"Jiří","full_name":"Friml, Jiří"}],"date_created":"2019-12-11T21:24:39Z","month":"12","page":"192","status":"public","department":[{"_id":"JiFr"}],"degree_awarded":"PhD","publisher":"Institute of Science and Technology Austria"},{"publication_identifier":{"eissn":["1664462X"]},"date_updated":"2023-09-06T14:33:46Z","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","external_id":{"pmid":["31803201"],"isi":["000499821700001"]},"scopus_import":"1","year":"2019","oa_version":"Published Version","has_accepted_license":"1","article_type":"original","volume":10,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"file_date_updated":"2020-07-14T12:47:52Z","oa":1,"publication_status":"published","date_published":"2019-11-14T00:00:00Z","_id":"7182","abstract":[{"lang":"eng","text":"During infection pathogens secrete small molecules, termed effectors, to manipulate and control the interaction with their specific hosts. Both the pathogen and the plant are under high selective pressure to rapidly adapt and co-evolve in what is usually referred to as molecular arms race. Components of the host’s immune system form a network that processes information about molecules with a foreign origin and damage-associated signals, integrating them with developmental and abiotic cues to adapt the plant’s responses. Both in the case of nucleotide-binding leucine-rich repeat receptors and leucine-rich repeat receptor kinases interaction networks have been extensively characterized. However, little is known on whether pathogenic effectors form complexes to overcome plant immunity and promote disease. Ustilago maydis, a biotrophic fungal pathogen that infects maize plants, produces effectors that target hubs in the immune network of the host cell. Here we assess the capability of U. maydis effector candidates to interact with each other, which may play a crucial role during the infection process. Using a systematic yeast-two-hybrid approach and based on a preliminary pooled screen, we selected 63 putative effectors for one-on-one matings with a library of nearly 300 effector candidates. We found that 126 of these effector candidates interacted either with themselves or other predicted effectors. Although the functional relevance of the observed interactions remains elusive, we propose that the observed abundance in complex formation between effectors adds an additional level of complexity to effector research and should be taken into consideration when studying effector evolution and function. Based on this fundamental finding, we suggest various scenarios which could evolutionarily drive the formation and stabilization of an effector interactome."}],"article_number":"1437","file":[{"content_type":"application/pdf","file_id":"7185","relation":"main_file","date_created":"2019-12-16T07:58:43Z","file_name":"2019_FrontiersPlant_Alcantara.pdf","creator":"dernst","file_size":1532505,"date_updated":"2020-07-14T12:47:52Z","access_level":"open_access","checksum":"995aa838aec2064d93550de82b40bbd1"}],"issue":"11","article_processing_charge":"No","doi":"10.3389/fpls.2019.01437","ddc":["580"],"language":[{"iso":"eng"}],"pmid":1,"author":[{"last_name":"Alcântara","first_name":"André","full_name":"Alcântara, André"},{"last_name":"Bosch","first_name":"Jason","full_name":"Bosch, Jason"},{"full_name":"Nazari, Fahimeh","first_name":"Fahimeh","last_name":"Nazari"},{"full_name":"Hoffmann, Gesa","first_name":"Gesa","last_name":"Hoffmann"},{"first_name":"Michelle C","full_name":"Gallei, Michelle C","last_name":"Gallei","orcid":"0000-0003-1286-7368","id":"35A03822-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Simon","full_name":"Uhse, Simon","last_name":"Uhse"},{"last_name":"Darino","first_name":"Martin A.","full_name":"Darino, Martin A."},{"last_name":"Olukayode","full_name":"Olukayode, Toluwase","first_name":"Toluwase"},{"last_name":"Reumann","first_name":"Daniel","full_name":"Reumann, Daniel"},{"last_name":"Baggaley","first_name":"Laura","full_name":"Baggaley, Laura"},{"full_name":"Djamei, Armin","first_name":"Armin","last_name":"Djamei"}],"type":"journal_article","day":"14","title":"Systematic Y2H screening reveals extensive effector-complex formation","citation":{"short":"A. Alcântara, J. Bosch, F. Nazari, G. Hoffmann, M.C. Gallei, S. Uhse, M.A. Darino, T. Olukayode, D. Reumann, L. Baggaley, A. Djamei, Frontiers in Plant Science 10 (2019).","apa":"Alcântara, A., Bosch, J., Nazari, F., Hoffmann, G., Gallei, M. C., Uhse, S., … Djamei, A. (2019). Systematic Y2H screening reveals extensive effector-complex formation. <i>Frontiers in Plant Science</i>. Frontiers. <a href=\"https://doi.org/10.3389/fpls.2019.01437\">https://doi.org/10.3389/fpls.2019.01437</a>","ama":"Alcântara A, Bosch J, Nazari F, et al. Systematic Y2H screening reveals extensive effector-complex formation. <i>Frontiers in Plant Science</i>. 2019;10(11). doi:<a href=\"https://doi.org/10.3389/fpls.2019.01437\">10.3389/fpls.2019.01437</a>","ieee":"A. Alcântara <i>et al.</i>, “Systematic Y2H screening reveals extensive effector-complex formation,” <i>Frontiers in Plant Science</i>, vol. 10, no. 11. Frontiers, 2019.","ista":"Alcântara A, Bosch J, Nazari F, Hoffmann G, Gallei MC, Uhse S, Darino MA, Olukayode T, Reumann D, Baggaley L, Djamei A. 2019. Systematic Y2H screening reveals extensive effector-complex formation. Frontiers in Plant Science. 10(11), 1437.","chicago":"Alcântara, André, Jason Bosch, Fahimeh Nazari, Gesa Hoffmann, Michelle C Gallei, Simon Uhse, Martin A. Darino, et al. “Systematic Y2H Screening Reveals Extensive Effector-Complex Formation.” <i>Frontiers in Plant Science</i>. Frontiers, 2019. <a href=\"https://doi.org/10.3389/fpls.2019.01437\">https://doi.org/10.3389/fpls.2019.01437</a>.","mla":"Alcântara, André, et al. “Systematic Y2H Screening Reveals Extensive Effector-Complex Formation.” <i>Frontiers in Plant Science</i>, vol. 10, no. 11, 1437, Frontiers, 2019, doi:<a href=\"https://doi.org/10.3389/fpls.2019.01437\">10.3389/fpls.2019.01437</a>."},"status":"public","intvolume":"        10","quality_controlled":"1","department":[{"_id":"JiFr"}],"publication":"Frontiers in Plant Science","isi":1,"publisher":"Frontiers","date_created":"2019-12-15T23:00:43Z","month":"11"},{"scopus_import":"1","external_id":{"isi":["000456336100050"],"pmid":["30610176"]},"date_updated":"2023-08-24T14:31:09Z","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","article_type":"original","year":"2019","oa_version":"Published Version","volume":116,"publication_status":"published","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1073/pnas.1818099116"}],"oa":1,"date_published":"2019-01-22T00:00:00Z","_id":"5908","abstract":[{"lang":"eng","text":"The interorganelle communication mediated by membrane contact sites (MCSs) is an evolutionary hallmark of eukaryotic cells. MCS connections enable the nonvesicular exchange of information between organelles and allow them to coordinate responses to changing cellular environments. In plants, the importance of MCS components in the responses to environmental stress has been widely established, but the molecular mechanisms regulating interorganelle connectivity during stress still remain opaque. In this report, we use the model plant Arabidopsis thaliana to show that ionic stress increases endoplasmic reticulum (ER)–plasma membrane (PM) connectivity by promoting the cortical expansion of synaptotagmin 1 (SYT1)-enriched ER–PM contact sites (S-EPCSs). We define differential roles for the cortical cytoskeleton in the regulation of S-EPCS dynamics and ER–PM connectivity, and we identify the accumulation of phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] at the PM as a molecular signal associated with the ER–PM connectivity changes. Our study highlights the functional conservation of EPCS components and PM phosphoinositides as modulators of ER–PM connectivity in eukaryotes, and uncovers unique aspects of the spatiotemporal regulation of ER–PM connectivity in plants."}],"article_processing_charge":"No","issue":"4","language":[{"iso":"eng"}],"doi":"10.1073/pnas.1818099116","pmid":1,"day":"22","author":[{"last_name":"Lee","first_name":"Eunkyoung","full_name":"Lee, Eunkyoung"},{"last_name":"Vanneste","full_name":"Vanneste, Steffen","first_name":"Steffen"},{"last_name":"Pérez-Sancho","full_name":"Pérez-Sancho, Jessica","first_name":"Jessica"},{"last_name":"Benitez-Fuente","full_name":"Benitez-Fuente, Francisco","first_name":"Francisco"},{"full_name":"Strelau, Matthew","first_name":"Matthew","last_name":"Strelau"},{"last_name":"Macho","full_name":"Macho, Alberto P.","first_name":"Alberto P."},{"full_name":"Botella, Miguel A.","first_name":"Miguel A.","last_name":"Botella"},{"first_name":"Jiří","full_name":"Friml, Jiří","last_name":"Friml","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Rosado, Abel","first_name":"Abel","last_name":"Rosado"}],"type":"journal_article","citation":{"ama":"Lee E, Vanneste S, Pérez-Sancho J, et al. Ionic stress enhances ER–PM connectivity via phosphoinositide-associated SYT1 contact site expansion in Arabidopsis. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. 2019;116(4):1420-1429. doi:<a href=\"https://doi.org/10.1073/pnas.1818099116\">10.1073/pnas.1818099116</a>","apa":"Lee, E., Vanneste, S., Pérez-Sancho, J., Benitez-Fuente, F., Strelau, M., Macho, A. P., … Rosado, A. (2019). Ionic stress enhances ER–PM connectivity via phosphoinositide-associated SYT1 contact site expansion in Arabidopsis. <i>Proceedings of the National Academy of Sciences of the United States of America</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.1818099116\">https://doi.org/10.1073/pnas.1818099116</a>","short":"E. Lee, S. Vanneste, J. Pérez-Sancho, F. Benitez-Fuente, M. Strelau, A.P. Macho, M.A. Botella, J. Friml, A. Rosado, Proceedings of the National Academy of Sciences of the United States of America 116 (2019) 1420–1429.","mla":"Lee, Eunkyoung, et al. “Ionic Stress Enhances ER–PM Connectivity via Phosphoinositide-Associated SYT1 Contact Site Expansion in Arabidopsis.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 116, no. 4, National Academy of Sciences, 2019, pp. 1420–29, doi:<a href=\"https://doi.org/10.1073/pnas.1818099116\">10.1073/pnas.1818099116</a>.","chicago":"Lee, Eunkyoung, Steffen Vanneste, Jessica Pérez-Sancho, Francisco Benitez-Fuente, Matthew Strelau, Alberto P. Macho, Miguel A. Botella, Jiří Friml, and Abel Rosado. “Ionic Stress Enhances ER–PM Connectivity via Phosphoinositide-Associated SYT1 Contact Site Expansion in Arabidopsis.” <i>Proceedings of the National Academy of Sciences of the United States of America</i>. National Academy of Sciences, 2019. <a href=\"https://doi.org/10.1073/pnas.1818099116\">https://doi.org/10.1073/pnas.1818099116</a>.","ista":"Lee E, Vanneste S, Pérez-Sancho J, Benitez-Fuente F, Strelau M, Macho AP, Botella MA, Friml J, Rosado A. 2019. Ionic stress enhances ER–PM connectivity via phosphoinositide-associated SYT1 contact site expansion in Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America. 116(4), 1420–1429.","ieee":"E. Lee <i>et al.</i>, “Ionic stress enhances ER–PM connectivity via phosphoinositide-associated SYT1 contact site expansion in Arabidopsis,” <i>Proceedings of the National Academy of Sciences of the United States of America</i>, vol. 116, no. 4. National Academy of Sciences, pp. 1420–1429, 2019."},"title":"Ionic stress enhances ER–PM connectivity via phosphoinositide-associated SYT1 contact site expansion in Arabidopsis","publication":"Proceedings of the National Academy of Sciences of the United States of America","department":[{"_id":"JiFr"}],"quality_controlled":"1","intvolume":"       116","status":"public","publisher":"National Academy of Sciences","isi":1,"month":"01","date_created":"2019-02-03T22:59:14Z","page":"1420-1429"},{"issue":"2","article_processing_charge":"No","date_published":"2019-02-08T00:00:00Z","_id":"6023","abstract":[{"lang":"eng","text":"Multicellular development requires coordinated cell polarization relative to body axes, and translation to oriented cell division 1–3 . In plants, it is unknown how cell polarities are connected to organismal axes and translated to division. Here, we identify Arabidopsis SOSEKI proteins that integrate apical–basal and radial organismal axes to localize to polar cell edges. Localization does not depend on tissue context, requires cell wall integrity and is defined by a transferrable, protein-specific motif. A Domain of Unknown Function in SOSEKI proteins resembles the DIX oligomerization domain in the animal Dishevelled polarity regulator. The DIX-like domain self-interacts and is required for edge localization and for influencing division orientation, together with a second domain that defines the polar membrane domain. Our work shows that SOSEKI proteins locally interpret global polarity cues and can influence cell division orientation. Furthermore, this work reveals that, despite fundamental differences, cell polarity mechanisms in plants and animals converge on a similar protein domain."}],"main_file_link":[{"open_access":"1","url":"https://www.biorxiv.org/content/10.1101/479113v1.abstract"}],"oa":1,"publication_status":"published","volume":5,"oa_version":"Submitted Version","year":"2019","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","date_updated":"2023-08-24T14:46:47Z","scopus_import":"1","external_id":{"isi":["000460479600014"]},"page":"160-166","date_created":"2019-02-17T22:59:21Z","month":"02","isi":1,"publisher":"Springer Nature","status":"public","intvolume":"         5","department":[{"_id":"JiFr"},{"_id":"EvBe"}],"quality_controlled":"1","publication":"Nature Plants","title":"A SOSEKI-based coordinate system interprets global polarity cues in arabidopsis","ec_funded":1,"citation":{"chicago":"Yoshida, Saiko, Alja Van Der Schuren, Maritza Van Dop, Luc Van Galen, Shunsuke Saiga, Milad Adibi, Barbara Möller, et al. “A SOSEKI-Based Coordinate System Interprets Global Polarity Cues in Arabidopsis.” <i>Nature Plants</i>. Springer Nature, 2019. <a href=\"https://doi.org/10.1038/s41477-019-0363-6\">https://doi.org/10.1038/s41477-019-0363-6</a>.","ieee":"S. Yoshida <i>et al.</i>, “A SOSEKI-based coordinate system interprets global polarity cues in arabidopsis,” <i>Nature Plants</i>, vol. 5, no. 2. Springer Nature, pp. 160–166, 2019.","ista":"Yoshida S, Van Der Schuren A, Van Dop M, Van Galen L, Saiga S, Adibi M, Möller B, Ten Hove CA, Marhavý P, Smith R, Friml J, Weijers D. 2019. A SOSEKI-based coordinate system interprets global polarity cues in arabidopsis. Nature Plants. 5(2), 160–166.","mla":"Yoshida, Saiko, et al. “A SOSEKI-Based Coordinate System Interprets Global Polarity Cues in Arabidopsis.” <i>Nature Plants</i>, vol. 5, no. 2, Springer Nature, 2019, pp. 160–66, doi:<a href=\"https://doi.org/10.1038/s41477-019-0363-6\">10.1038/s41477-019-0363-6</a>.","short":"S. Yoshida, A. Van Der Schuren, M. Van Dop, L. Van Galen, S. Saiga, M. Adibi, B. Möller, C.A. Ten Hove, P. Marhavý, R. Smith, J. Friml, D. Weijers, Nature Plants 5 (2019) 160–166.","ama":"Yoshida S, Van Der Schuren A, Van Dop M, et al. A SOSEKI-based coordinate system interprets global polarity cues in arabidopsis. <i>Nature Plants</i>. 2019;5(2):160-166. doi:<a href=\"https://doi.org/10.1038/s41477-019-0363-6\">10.1038/s41477-019-0363-6</a>","apa":"Yoshida, S., Van Der Schuren, A., Van Dop, M., Van Galen, L., Saiga, S., Adibi, M., … Weijers, D. (2019). A SOSEKI-based coordinate system interprets global polarity cues in arabidopsis. <i>Nature Plants</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41477-019-0363-6\">https://doi.org/10.1038/s41477-019-0363-6</a>"},"type":"journal_article","author":[{"last_name":"Yoshida","first_name":"Saiko","full_name":"Yoshida, Saiko","id":"2E46069C-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Van Der Schuren","first_name":"Alja","full_name":"Van Der Schuren, Alja"},{"last_name":"Van Dop","full_name":"Van Dop, Maritza","first_name":"Maritza"},{"first_name":"Luc","full_name":"Van Galen, Luc","last_name":"Van Galen"},{"first_name":"Shunsuke","full_name":"Saiga, Shunsuke","last_name":"Saiga"},{"last_name":"Adibi","full_name":"Adibi, Milad","first_name":"Milad"},{"last_name":"Möller","first_name":"Barbara","full_name":"Möller, Barbara"},{"last_name":"Ten Hove","first_name":"Colette A.","full_name":"Ten Hove, Colette A."},{"full_name":"Marhavy, Peter","first_name":"Peter","last_name":"Marhavy","id":"3F45B078-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5227-5741"},{"last_name":"Smith","full_name":"Smith, Richard","first_name":"Richard"},{"last_name":"Friml","full_name":"Friml, Jiří","first_name":"Jiří","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Weijers, Dolf","first_name":"Dolf","last_name":"Weijers"}],"day":"08","project":[{"_id":"25681D80-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","grant_number":"291734","name":"International IST Postdoc Fellowship Programme"}],"doi":"10.1038/s41477-019-0363-6","language":[{"iso":"eng"}]},{"isi":1,"publisher":"Oxford University Press","status":"public","intvolume":"        60","department":[{"_id":"JiFr"}],"quality_controlled":"1","publication":"Plant and Cell Physiology","page":"255-273","date_created":"2019-03-17T22:59:14Z","month":"02","pmid":1,"doi":"10.1093/pcp/pcz001","language":[{"iso":"eng"}],"title":"Root adaptation to H2O2-induced oxidative stress by ARF-GEF BEN1- and cytoskeleton-mediated PIN2 trafficking","citation":{"short":"M. Zwiewka, A. Bielach, P. Tamizhselvan, S. Madhavan, E.E. Ryad, S. Tan, M. Hrtyan, P. Dobrev, R. Vanková, J. Friml, V.B. Tognetti, Plant and Cell Physiology 60 (2019) 255–273.","ama":"Zwiewka M, Bielach A, Tamizhselvan P, et al. Root adaptation to H2O2-induced oxidative stress by ARF-GEF BEN1- and cytoskeleton-mediated PIN2 trafficking. <i>Plant and Cell Physiology</i>. 2019;60(2):255-273. doi:<a href=\"https://doi.org/10.1093/pcp/pcz001\">10.1093/pcp/pcz001</a>","apa":"Zwiewka, M., Bielach, A., Tamizhselvan, P., Madhavan, S., Ryad, E. E., Tan, S., … Tognetti, V. B. (2019). Root adaptation to H2O2-induced oxidative stress by ARF-GEF BEN1- and cytoskeleton-mediated PIN2 trafficking. <i>Plant and Cell Physiology</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/pcp/pcz001\">https://doi.org/10.1093/pcp/pcz001</a>","chicago":"Zwiewka, Marta, Agnieszka Bielach, Prashanth Tamizhselvan, Sharmila Madhavan, Eman Elrefaay Ryad, Shutang Tan, Mónika Hrtyan, et al. “Root Adaptation to H2O2-Induced Oxidative Stress by ARF-GEF BEN1- and Cytoskeleton-Mediated PIN2 Trafficking.” <i>Plant and Cell Physiology</i>. Oxford University Press, 2019. <a href=\"https://doi.org/10.1093/pcp/pcz001\">https://doi.org/10.1093/pcp/pcz001</a>.","ieee":"M. Zwiewka <i>et al.</i>, “Root adaptation to H2O2-induced oxidative stress by ARF-GEF BEN1- and cytoskeleton-mediated PIN2 trafficking,” <i>Plant and Cell Physiology</i>, vol. 60, no. 2. Oxford University Press, pp. 255–273, 2019.","ista":"Zwiewka M, Bielach A, Tamizhselvan P, Madhavan S, Ryad EE, Tan S, Hrtyan M, Dobrev P, Vanková R, Friml J, Tognetti VB. 2019. Root adaptation to H2O2-induced oxidative stress by ARF-GEF BEN1- and cytoskeleton-mediated PIN2 trafficking. Plant and Cell Physiology. 60(2), 255–273.","mla":"Zwiewka, Marta, et al. “Root Adaptation to H2O2-Induced Oxidative Stress by ARF-GEF BEN1- and Cytoskeleton-Mediated PIN2 Trafficking.” <i>Plant and Cell Physiology</i>, vol. 60, no. 2, Oxford University Press, 2019, pp. 255–73, doi:<a href=\"https://doi.org/10.1093/pcp/pcz001\">10.1093/pcp/pcz001</a>."},"author":[{"first_name":"Marta","full_name":"Zwiewka, Marta","last_name":"Zwiewka"},{"full_name":"Bielach, Agnieszka","first_name":"Agnieszka","last_name":"Bielach"},{"full_name":"Tamizhselvan, Prashanth","first_name":"Prashanth","last_name":"Tamizhselvan"},{"last_name":"Madhavan","full_name":"Madhavan, Sharmila","first_name":"Sharmila"},{"full_name":"Ryad, Eman Elrefaay","first_name":"Eman Elrefaay","last_name":"Ryad"},{"full_name":"Tan, Shutang","first_name":"Shutang","last_name":"Tan","orcid":"0000-0002-0471-8285","id":"2DE75584-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Hrtyan","first_name":"Mónika","full_name":"Hrtyan, Mónika","id":"45A71A74-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Dobrev","first_name":"Petre","full_name":"Dobrev, Petre"},{"last_name":"Vanková","full_name":"Vanková, Radomira","first_name":"Radomira"},{"last_name":"Friml","first_name":"Jiří","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596"},{"last_name":"Tognetti","first_name":"Vanesa B.","full_name":"Tognetti, Vanesa B."}],"type":"journal_article","day":"01","publication_status":"published","volume":60,"issue":"2","article_processing_charge":"No","_id":"6104","date_published":"2019-02-01T00:00:00Z","abstract":[{"lang":"eng","text":"Abiotic stress poses constant challenges for plant survival and is a serious problem for global agricultural productivity. On a molecular level, stress conditions result in elevation of reactive oxygen species (ROS) production causing oxidative stress associated with oxidation of proteins and nucleic acids as well as impairment of membrane functions. Adaptation of root growth to ROS accumulation is facilitated through modification of auxin and cytokinin hormone homeostasis. Here, we report that in Arabidopsis root meristem, ROS-induced changes of auxin levels correspond to decreased abundance of PIN auxin efflux carriers at the plasma membrane (PM). Specifically, increase in H2O2 levels affects PIN2 endocytic recycling. We show that the PIN2 intracellular trafficking during adaptation to oxidative stress requires the function of the ADP-ribosylation factor (ARF)-guanine-nucleotide exchange factor (GEF) BEN1, an actin-associated regulator of the trafficking from the PM to early endosomes and, presumably, indirectly, trafficking to the vacuoles. We propose that H2O2 levels affect the actin dynamics thus modulating ARF-GEF-dependent trafficking of PIN2. This mechanism provides a way how root growth acclimates to stress and adapts to a changing environment."}],"publication_identifier":{"issn":["0032-0781"],"eissn":["1471-9053"]},"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","date_updated":"2023-08-25T08:05:28Z","external_id":{"isi":["000459634300002"],"pmid":["30668780"]},"scopus_import":"1","oa_version":"None","year":"2019"},{"article_processing_charge":"No","date_published":"2019-04-11T00:00:00Z","_id":"6259","abstract":[{"lang":"eng","text":"The plant hormone auxin has crucial roles in almost all aspects of plant growth and development. Concentrations of auxin vary across different tissues, mediating distinct developmental outcomes and contributing to the functional diversity of auxin. However, the mechanisms that underlie these activities are poorly understood. Here we identify an auxin signalling mechanism, which acts in parallel to the canonical auxin pathway based on the transport inhibitor response1 (TIR1) and other auxin receptor F-box (AFB) family proteins (TIR1/AFB receptors)1,2, that translates levels of cellular auxin to mediate differential growth during apical-hook development. This signalling mechanism operates at the concave side of the apical hook, and involves auxin-mediated C-terminal cleavage of transmembrane kinase 1 (TMK1). The cytosolic and nucleus-translocated C terminus of TMK1 specifically interacts with and phosphorylates two non-canonical transcriptional repressors of the auxin or indole-3-acetic acid (Aux/IAA) family (IAA32 and IAA34), thereby regulating ARF transcription factors. In contrast to the degradation of Aux/IAA transcriptional repressors in the canonical pathway, the newly identified mechanism stabilizes the non-canonical IAA32 and IAA34 transcriptional repressors to regulate gene expression and ultimately inhibit growth. The auxin–TMK1 signalling pathway originates at the cell surface, is triggered by high levels of auxin and shares a partially overlapping set of transcription factors with the TIR1/AFB signalling pathway. This allows distinct interpretations of different concentrations of cellular auxin, and thus enables this versatile signalling molecule to mediate complex developmental outcomes."}],"file":[{"checksum":"6b84ab602a34382cf0340a37a1378c75","access_level":"open_access","date_updated":"2020-11-13T07:37:41Z","file_size":4321328,"creator":"dernst","file_name":"2019_Nature _Cao_accepted.pdf","date_created":"2020-11-13T07:37:41Z","success":1,"file_id":"8751","relation":"main_file","content_type":"application/pdf"}],"oa":1,"publication_status":"published","volume":568,"file_date_updated":"2020-11-13T07:37:41Z","oa_version":"Submitted Version","has_accepted_license":"1","year":"2019","article_type":"original","publication_identifier":{"issn":["0028-0836"],"eissn":["1476-4687"]},"external_id":{"pmid":["30944466"],"isi":["000464412700050"]},"scopus_import":"1","date_updated":"2023-09-05T14:58:41Z","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","page":"240-243","date_created":"2019-04-09T08:37:05Z","month":"04","isi":1,"publisher":"Springer Nature","status":"public","intvolume":"       568","publication":"Nature","department":[{"_id":"JiFr"}],"quality_controlled":"1","title":"TMK1-mediated auxin signalling regulates differential growth of the apical hook","citation":{"short":"M. Cao, R. Chen, P. Li, Y. Yu, R. Zheng, D. Ge, W. Zheng, X. Wang, Y. Gu, Z. Gelová, J. Friml, H. Zhang, R. Liu, J. He, T. Xu, Nature 568 (2019) 240–243.","ama":"Cao M, Chen R, Li P, et al. TMK1-mediated auxin signalling regulates differential growth of the apical hook. <i>Nature</i>. 2019;568:240-243. doi:<a href=\"https://doi.org/10.1038/s41586-019-1069-7\">10.1038/s41586-019-1069-7</a>","apa":"Cao, M., Chen, R., Li, P., Yu, Y., Zheng, R., Ge, D., … Xu, T. (2019). TMK1-mediated auxin signalling regulates differential growth of the apical hook. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-019-1069-7\">https://doi.org/10.1038/s41586-019-1069-7</a>","chicago":"Cao, Min, Rong Chen, Pan Li, Yongqiang Yu, Rui Zheng, Danfeng Ge, Wei Zheng, et al. “TMK1-Mediated Auxin Signalling Regulates Differential Growth of the Apical Hook.” <i>Nature</i>. Springer Nature, 2019. <a href=\"https://doi.org/10.1038/s41586-019-1069-7\">https://doi.org/10.1038/s41586-019-1069-7</a>.","ieee":"M. Cao <i>et al.</i>, “TMK1-mediated auxin signalling regulates differential growth of the apical hook,” <i>Nature</i>, vol. 568. Springer Nature, pp. 240–243, 2019.","ista":"Cao M, Chen R, Li P, Yu Y, Zheng R, Ge D, Zheng W, Wang X, Gu Y, Gelová Z, Friml J, Zhang H, Liu R, He J, Xu T. 2019. TMK1-mediated auxin signalling regulates differential growth of the apical hook. Nature. 568, 240–243.","mla":"Cao, Min, et al. “TMK1-Mediated Auxin Signalling Regulates Differential Growth of the Apical Hook.” <i>Nature</i>, vol. 568, Springer Nature, 2019, pp. 240–43, doi:<a href=\"https://doi.org/10.1038/s41586-019-1069-7\">10.1038/s41586-019-1069-7</a>."},"ec_funded":1,"type":"journal_article","author":[{"first_name":"Min","full_name":"Cao, Min","last_name":"Cao"},{"first_name":"Rong","full_name":"Chen, Rong","last_name":"Chen"},{"full_name":"Li, Pan","first_name":"Pan","last_name":"Li"},{"last_name":"Yu","first_name":"Yongqiang","full_name":"Yu, Yongqiang"},{"first_name":"Rui","full_name":"Zheng, Rui","last_name":"Zheng"},{"last_name":"Ge","first_name":"Danfeng","full_name":"Ge, Danfeng"},{"first_name":"Wei","full_name":"Zheng, Wei","last_name":"Zheng"},{"last_name":"Wang","full_name":"Wang, Xuhui","first_name":"Xuhui"},{"last_name":"Gu","first_name":"Yangtao","full_name":"Gu, Yangtao"},{"orcid":"0000-0003-4783-1752","id":"0AE74790-0E0B-11E9-ABC7-1ACFE5697425","last_name":"Gelová","full_name":"Gelová, Zuzana","first_name":"Zuzana"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","last_name":"Friml","full_name":"Friml, Jiří","first_name":"Jiří"},{"full_name":"Zhang, Heng","first_name":"Heng","last_name":"Zhang"},{"last_name":"Liu","first_name":"Renyi","full_name":"Liu, Renyi"},{"full_name":"He, Jun","first_name":"Jun","last_name":"He"},{"last_name":"Xu","first_name":"Tongda","full_name":"Xu, Tongda"}],"day":"11","project":[{"_id":"261099A6-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","grant_number":"742985"}],"pmid":1,"related_material":{"link":[{"relation":"press_release","url":"https://ist.ac.at/en/news/newly-discovered-mechanism-of-plant-hormone-auxin-acts-the-opposite-way/","description":"News on IST Homepage"}]},"ddc":["580"],"doi":"10.1038/s41586-019-1069-7","language":[{"iso":"eng"}]},{"title":"Pinstatic acid promotes auxin transport by inhibiting PIN internalization","ec_funded":1,"citation":{"ista":"Oochi A, Hajny J, Fukui K, Nakao Y, Gallei MC, Quareshy M, Takahashi K, Kinoshita T, Harborough S, Kepinski S, Kasahara H, Napier R, Friml J, Hayashi K. 2019. Pinstatic acid promotes auxin transport by inhibiting PIN internalization. Plant Physiology. 180(2), 1152–1165.","ieee":"A. Oochi <i>et al.</i>, “Pinstatic acid promotes auxin transport by inhibiting PIN internalization,” <i>Plant Physiology</i>, vol. 180, no. 2. ASPB, pp. 1152–1165, 2019.","chicago":"Oochi, A, Jakub Hajny, K Fukui, Y Nakao, Michelle C Gallei, M Quareshy, K Takahashi, et al. “Pinstatic Acid Promotes Auxin Transport by Inhibiting PIN Internalization.” <i>Plant Physiology</i>. ASPB, 2019. <a href=\"https://doi.org/10.1104/pp.19.00201\">https://doi.org/10.1104/pp.19.00201</a>.","mla":"Oochi, A., et al. “Pinstatic Acid Promotes Auxin Transport by Inhibiting PIN Internalization.” <i>Plant Physiology</i>, vol. 180, no. 2, ASPB, 2019, pp. 1152–65, doi:<a href=\"https://doi.org/10.1104/pp.19.00201\">10.1104/pp.19.00201</a>.","short":"A. Oochi, J. Hajny, K. Fukui, Y. Nakao, M.C. Gallei, M. Quareshy, K. Takahashi, T. Kinoshita, S. Harborough, S. Kepinski, H. Kasahara, R. Napier, J. Friml, K. Hayashi, Plant Physiology 180 (2019) 1152–1165.","apa":"Oochi, A., Hajny, J., Fukui, K., Nakao, Y., Gallei, M. C., Quareshy, M., … Hayashi, K. (2019). Pinstatic acid promotes auxin transport by inhibiting PIN internalization. <i>Plant Physiology</i>. ASPB. <a href=\"https://doi.org/10.1104/pp.19.00201\">https://doi.org/10.1104/pp.19.00201</a>","ama":"Oochi A, Hajny J, Fukui K, et al. Pinstatic acid promotes auxin transport by inhibiting PIN internalization. <i>Plant Physiology</i>. 2019;180(2):1152-1165. doi:<a href=\"https://doi.org/10.1104/pp.19.00201\">10.1104/pp.19.00201</a>"},"type":"journal_article","author":[{"full_name":"Oochi, A","first_name":"A","last_name":"Oochi"},{"full_name":"Hajny, Jakub","first_name":"Jakub","last_name":"Hajny","orcid":"0000-0003-2140-7195","id":"4800CC20-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Fukui","first_name":"K","full_name":"Fukui, K"},{"last_name":"Nakao","full_name":"Nakao, Y","first_name":"Y"},{"last_name":"Gallei","first_name":"Michelle C","full_name":"Gallei, Michelle C","orcid":"0000-0003-1286-7368","id":"35A03822-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Quareshy","first_name":"M","full_name":"Quareshy, M"},{"last_name":"Takahashi","full_name":"Takahashi, K","first_name":"K"},{"full_name":"Kinoshita, T","first_name":"T","last_name":"Kinoshita"},{"last_name":"Harborough","full_name":"Harborough, SR","first_name":"SR"},{"full_name":"Kepinski, S","first_name":"S","last_name":"Kepinski"},{"last_name":"Kasahara","full_name":"Kasahara, H","first_name":"H"},{"last_name":"Napier","full_name":"Napier, RM","first_name":"RM"},{"first_name":"Jiří","full_name":"Friml, Jiří","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596"},{"last_name":"Hayashi","full_name":"Hayashi, KI","first_name":"KI"}],"day":"01","acknowledgement":"We thank Dr. H. Fukaki (University of Kobe), Dr. R. Offringa (Leiden University), Dr. Jianwei Pan (Zhejiang Normal University), and Dr. M. Estelle (University of California at San Diego) for providing mutants and transgenic line seeds.\r\nThis work was supported by the Ministry of Education, Culture, Sports, Science, and Technology (Grant-in-Aid for Scientific Research no. JP25114518 to K.H.), the Biotechnology and Biological Sciences Research Council (award no. BB/L009366/1 to R.N. and S.K.), and the European Union’s Horizon2020 program (European Research Council grant agreement no. 742985 to J.F.).","pmid":1,"project":[{"name":"Tracing Evolution of Auxin Transport and Polarity in Plants","grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425","call_identifier":"H2020"}],"related_material":{"record":[{"status":"public","id":"11626","relation":"dissertation_contains"},{"relation":"dissertation_contains","id":"8822","status":"public"}]},"doi":"10.1104/pp.19.00201","language":[{"iso":"eng"}],"page":"1152-1165","date_created":"2019-04-09T08:38:20Z","month":"06","isi":1,"publisher":"ASPB","intvolume":"       180","status":"public","department":[{"_id":"JiFr"}],"quality_controlled":"1","publication":"Plant Physiology","year":"2019","oa_version":"Published Version","article_type":"original","publication_identifier":{"issn":["0032-0889"],"eissn":["1532-2548"]},"date_updated":"2024-03-25T23:30:21Z","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","scopus_import":"1","external_id":{"isi":["000470086100045"],"pmid":["30936248"]},"issue":"2","article_processing_charge":"No","_id":"6260","date_published":"2019-06-01T00:00:00Z","abstract":[{"text":"Polar auxin transport plays a pivotal role in plant growth and development. PIN auxin efflux carriers regulate directional auxin movement by establishing local auxin maxima, minima, and gradients that drive multiple developmental processes and responses to environmental signals. Auxin has been proposed to modulate its own transport by regulating subcellular PIN trafficking via processes such as clathrin-mediated PIN endocytosis and constitutive recycling. Here, we further investigated the mechanisms by which auxin affects PIN trafficking by screening auxin analogs and identified pinstatic acid (PISA) as a positive modulator of polar auxin transport in Arabidopsis thaliana. PISA had an auxin-like effect on hypocotyl elongation and adventitious root formation via positive regulation of auxin transport. PISA did not activate SCFTIR1/AFB signaling and yet induced PIN accumulation at the cell surface by inhibiting PIN internalization from the plasma membrane. This work demonstrates PISA to be a promising chemical tool to dissect the regulatory mechanisms behind subcellular PIN trafficking and auxin transport.","lang":"eng"}],"oa":1,"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1104/pp.19.00201"}],"publication_status":"published","volume":180},{"scopus_import":"1","external_id":{"isi":["000466860800010"],"pmid":["30787134"]},"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","date_updated":"2023-08-25T10:10:23Z","publication_identifier":{"eissn":["1532-2548"],"issn":["0032-0889"]},"article_type":"letter_note","oa_version":"Published Version","year":"2019","volume":180,"publication_status":"published","oa":1,"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1104/pp.18.01305"}],"_id":"6261","abstract":[{"lang":"eng","text":"Nitrate regulation of root stem cell activity is auxin-dependent."}],"date_published":"2019-05-01T00:00:00Z","article_processing_charge":"No","issue":"1","language":[{"iso":"eng"}],"doi":"10.1104/pp.18.01305","pmid":1,"day":"01","type":"journal_article","author":[{"last_name":"Wang","full_name":"Wang, Y","first_name":"Y"},{"last_name":"Gong","full_name":"Gong, Z","first_name":"Z"},{"last_name":"Friml","first_name":"Jiří","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596"},{"full_name":"Zhang, J","first_name":"J","last_name":"Zhang"}],"citation":{"ieee":"Y. Wang, Z. Gong, J. Friml, and J. Zhang, “Nitrate modulates the differentiation of root distal stem cells,” <i>Plant Physiology</i>, vol. 180, no. 1. ASPB, pp. 22–25, 2019.","ista":"Wang Y, Gong Z, Friml J, Zhang J. 2019. Nitrate modulates the differentiation of root distal stem cells. Plant Physiology. 180(1), 22–25.","chicago":"Wang, Y, Z Gong, Jiří Friml, and J Zhang. “Nitrate Modulates the Differentiation of Root Distal Stem Cells.” <i>Plant Physiology</i>. ASPB, 2019. <a href=\"https://doi.org/10.1104/pp.18.01305\">https://doi.org/10.1104/pp.18.01305</a>.","mla":"Wang, Y., et al. “Nitrate Modulates the Differentiation of Root Distal Stem Cells.” <i>Plant Physiology</i>, vol. 180, no. 1, ASPB, 2019, pp. 22–25, doi:<a href=\"https://doi.org/10.1104/pp.18.01305\">10.1104/pp.18.01305</a>.","short":"Y. Wang, Z. Gong, J. Friml, J. Zhang, Plant Physiology 180 (2019) 22–25.","apa":"Wang, Y., Gong, Z., Friml, J., &#38; Zhang, J. (2019). Nitrate modulates the differentiation of root distal stem cells. <i>Plant Physiology</i>. ASPB. <a href=\"https://doi.org/10.1104/pp.18.01305\">https://doi.org/10.1104/pp.18.01305</a>","ama":"Wang Y, Gong Z, Friml J, Zhang J. Nitrate modulates the differentiation of root distal stem cells. <i>Plant Physiology</i>. 2019;180(1):22-25. doi:<a href=\"https://doi.org/10.1104/pp.18.01305\">10.1104/pp.18.01305</a>"},"title":"Nitrate modulates the differentiation of root distal stem cells","publication":"Plant Physiology","department":[{"_id":"JiFr"}],"quality_controlled":"1","status":"public","intvolume":"       180","publisher":"ASPB","isi":1,"month":"05","date_created":"2019-04-09T08:46:17Z","page":"22-25"},{"article_processing_charge":"Yes (via OA deal)","issue":"6","_id":"6262","date_published":"2019-06-01T00:00:00Z","abstract":[{"text":"Gravitropism is an adaptive response that orients plant growth parallel to the gravity vector. Asymmetric\r\ndistribution of the phytohormone auxin is a necessary prerequisite to the tropic bending both in roots and\r\nshoots. During hypocotyl gravitropic response, the PIN3 auxin transporter polarizes within gravity-sensing\r\ncells to redirect intercellular auxin fluxes. First gravity-induced PIN3 polarization to the bottom cell mem-\r\nbranes leads to the auxin accumulation at the lower side of the organ, initiating bending and, later, auxin\r\nfeedback-mediated repolarization restores symmetric auxin distribution to terminate bending. Here, we per-\r\nformed a forward genetic screen to identify regulators of both PIN3 polarization events during gravitropic\r\nresponse. We searched for mutants with defective PIN3 polarizations based on easy-to-score morphological\r\noutputs of decreased or increased gravity-induced hypocotyl bending. We identified the number of\r\nhypocotyl reduced bending (hrb) and hypocotyl hyperbending (hhb) mutants, revealing that reduced bending corre-\r\nlated typically with defective gravity-induced PIN3 relocation whereas all analyzed hhb mutants showed\r\ndefects in the second, auxin-mediated PIN3 relocation. Next-generation sequencing-aided mutation map-\r\nping identified several candidate genes, including SCARECROW and ACTIN2, revealing roles of endodermis\r\nspecification and actin cytoskeleton in the respective gravity- and auxin-induced PIN polarization events.\r\nThe hypocotyl gravitropism screen thus promises to provide novel insights into mechanisms underlying cell\r\npolarity and plant adaptive development.","lang":"eng"}],"file":[{"content_type":"application/pdf","relation":"main_file","file_id":"6304","date_created":"2019-04-15T09:38:43Z","file_name":"2019_PlantJournal_Rakusov.pdf","creator":"dernst","file_size":1383100,"date_updated":"2020-07-14T12:47:25Z","access_level":"open_access","checksum":"ad3b5e270b67ba2a45f894ce3be27920"}],"oa":1,"publication_status":"published","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"volume":98,"file_date_updated":"2020-07-14T12:47:25Z","year":"2019","oa_version":"Published Version","has_accepted_license":"1","article_type":"original","publication_identifier":{"eissn":["1365-313x"],"issn":["0960-7412"]},"scopus_import":"1","external_id":{"pmid":["30821050"],"isi":["000473644100008"]},"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","date_updated":"2025-05-07T11:12:30Z","page":"1048-1059","date_created":"2019-04-09T08:46:44Z","month":"06","isi":1,"publisher":"Wiley","intvolume":"        98","status":"public","publication":"The Plant Journal","quality_controlled":"1","department":[{"_id":"JiFr"}],"title":"Genetic screen for factors mediating PIN polarization in gravistimulated Arabidopsis thaliana hypocotyls","citation":{"short":"H. Rakusová, H. Han, P. Valošek, J. Friml, The Plant Journal 98 (2019) 1048–1059.","apa":"Rakusová, H., Han, H., Valošek, P., &#38; Friml, J. (2019). Genetic screen for factors mediating PIN polarization in gravistimulated Arabidopsis thaliana hypocotyls. <i>The Plant Journal</i>. Wiley. <a href=\"https://doi.org/10.1111/tpj.14301\">https://doi.org/10.1111/tpj.14301</a>","ama":"Rakusová H, Han H, Valošek P, Friml J. Genetic screen for factors mediating PIN polarization in gravistimulated Arabidopsis thaliana hypocotyls. <i>The Plant Journal</i>. 2019;98(6):1048-1059. doi:<a href=\"https://doi.org/10.1111/tpj.14301\">10.1111/tpj.14301</a>","ieee":"H. Rakusová, H. Han, P. Valošek, and J. Friml, “Genetic screen for factors mediating PIN polarization in gravistimulated Arabidopsis thaliana hypocotyls,” <i>The Plant Journal</i>, vol. 98, no. 6. Wiley, pp. 1048–1059, 2019.","ista":"Rakusová H, Han H, Valošek P, Friml J. 2019. Genetic screen for factors mediating PIN polarization in gravistimulated Arabidopsis thaliana hypocotyls. The Plant Journal. 98(6), 1048–1059.","chicago":"Rakusová, Hana, Huibin Han, Petr Valošek, and Jiří Friml. “Genetic Screen for Factors Mediating PIN Polarization in Gravistimulated Arabidopsis Thaliana Hypocotyls.” <i>The Plant Journal</i>. Wiley, 2019. <a href=\"https://doi.org/10.1111/tpj.14301\">https://doi.org/10.1111/tpj.14301</a>.","mla":"Rakusová, Hana, et al. “Genetic Screen for Factors Mediating PIN Polarization in Gravistimulated Arabidopsis Thaliana Hypocotyls.” <i>The Plant Journal</i>, vol. 98, no. 6, Wiley, 2019, pp. 1048–59, doi:<a href=\"https://doi.org/10.1111/tpj.14301\">10.1111/tpj.14301</a>."},"ec_funded":1,"type":"journal_article","author":[{"last_name":"Rakusová","full_name":"Rakusová, Hana","first_name":"Hana"},{"id":"31435098-F248-11E8-B48F-1D18A9856A87","full_name":"Han, Huibin","first_name":"Huibin","last_name":"Han"},{"id":"3CDB6F94-F248-11E8-B48F-1D18A9856A87","last_name":"Valošek","first_name":"Petr","full_name":"Valošek, Petr"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří","first_name":"Jiří","last_name":"Friml"}],"day":"01","pmid":1,"project":[{"name":"Polarity and subcellular dynamics in plants","grant_number":"282300","call_identifier":"FP7","_id":"25716A02-B435-11E9-9278-68D0E5697425"}],"ddc":["580"],"doi":"10.1111/tpj.14301","language":[{"iso":"eng"}]}]