[{"scopus_import":"1","volume":11,"article_number":"2170","oa":1,"intvolume":"        11","acknowledgement":"We thank Daria Siekhaus, Jiri Friml and Alexander Johnson for critical reading of the manuscript, Peter Pimpl, Christian Luschnig and Liwen Jiang for sharing published material, Lesia Rodriguez Solovey for technical assistance. This work was supported by the Austrian Science Fund (FWF01_I1774S) to A.H., K.Ö., and E.B., the German Research Foundation (DFG; He3424/6-1 to I.H.), by the People Programme (Marie Curie Actions) of the European Union’s Seventh Framework Programme (FP7/2007-2013) under REA grant agreement n° [291734] (to N.C.), by the EU in the framework of the Marie-Curie FP7 COFUND People Programme through the award of an AgreenSkills+ fellowship No. 609398 (to J.S.) and by the Scientific Service Units of IST-Austria through resources provided by the Bioimaging Facility, the Life Science Facility. The IJPB benefits from the support of Saclay Plant Sciences-SPS (ANR-17-EUR-0007).","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","publication":"Nature Communications","file_date_updated":"2020-10-06T07:47:53Z","project":[{"name":"Hormone cross-talk drives nutrient dependent plant development","grant_number":"I 1774-B16","_id":"2542D156-B435-11E9-9278-68D0E5697425","call_identifier":"FWF"},{"grant_number":"291734","_id":"25681D80-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","name":"International IST Postdoc Fellowship Programme"}],"_id":"7805","external_id":{"pmid":["32358503"],"isi":["000531425900012"]},"date_created":"2020-05-10T22:00:48Z","title":"Synergistic on Auxin and Cytokinin 1 positively regulates growth and attenuates soil pathogen resistance","date_updated":"2023-08-21T06:21:56Z","month":"05","article_processing_charge":"No","date_published":"2020-05-01T00:00:00Z","ec_funded":1,"file":[{"file_name":"2020_NatureComm_Hurny.pdf","access_level":"open_access","success":1,"relation":"main_file","checksum":"2cba327c9e9416d75cb96be54b0fb441","file_size":4743576,"date_updated":"2020-10-06T07:47:53Z","content_type":"application/pdf","creator":"dernst","date_created":"2020-10-06T07:47:53Z","file_id":"8614"}],"citation":{"ista":"Hurny A, Cuesta C, Cavallari N, Ötvös K, Duclercq J, Dokládal L, Montesinos López JC, Gallemi M, Semerádová H, Rauter T, Stenzel I, Persiau G, Benade F, Bhalearo R, Sýkorová E, Gorzsás A, Sechet J, Mouille G, Heilmann I, De Jaeger G, Ludwig-Müller J, Benková E. 2020. Synergistic on Auxin and Cytokinin 1 positively regulates growth and attenuates soil pathogen resistance. Nature Communications. 11, 2170.","mla":"Hurny, Andrej, et al. “Synergistic on Auxin and Cytokinin 1 Positively Regulates Growth and Attenuates Soil Pathogen Resistance.” <i>Nature Communications</i>, vol. 11, 2170, Springer Nature, 2020, doi:<a href=\"https://doi.org/10.1038/s41467-020-15895-5\">10.1038/s41467-020-15895-5</a>.","chicago":"Hurny, Andrej, Candela Cuesta, Nicola Cavallari, Krisztina Ötvös, Jerome Duclercq, Ladislav Dokládal, Juan C Montesinos López, et al. “Synergistic on Auxin and Cytokinin 1 Positively Regulates Growth and Attenuates Soil Pathogen Resistance.” <i>Nature Communications</i>. Springer Nature, 2020. <a href=\"https://doi.org/10.1038/s41467-020-15895-5\">https://doi.org/10.1038/s41467-020-15895-5</a>.","ama":"Hurny A, Cuesta C, Cavallari N, et al. Synergistic on Auxin and Cytokinin 1 positively regulates growth and attenuates soil pathogen resistance. <i>Nature Communications</i>. 2020;11. doi:<a href=\"https://doi.org/10.1038/s41467-020-15895-5\">10.1038/s41467-020-15895-5</a>","ieee":"A. Hurny <i>et al.</i>, “Synergistic on Auxin and Cytokinin 1 positively regulates growth and attenuates soil pathogen resistance,” <i>Nature Communications</i>, vol. 11. Springer Nature, 2020.","apa":"Hurny, A., Cuesta, C., Cavallari, N., Ötvös, K., Duclercq, J., Dokládal, L., … Benková, E. (2020). Synergistic on Auxin and Cytokinin 1 positively regulates growth and attenuates soil pathogen resistance. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-020-15895-5\">https://doi.org/10.1038/s41467-020-15895-5</a>","short":"A. Hurny, C. Cuesta, N. Cavallari, K. Ötvös, J. Duclercq, L. Dokládal, J.C. Montesinos López, M. Gallemi, H. Semerádová, T. Rauter, I. Stenzel, G. Persiau, F. Benade, R. Bhalearo, E. Sýkorová, A. Gorzsás, J. Sechet, G. Mouille, I. Heilmann, G. De Jaeger, J. Ludwig-Müller, E. Benková, Nature Communications 11 (2020)."},"type":"journal_article","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"ddc":["570"],"article_type":"original","publication_status":"published","abstract":[{"text":"Plants as non-mobile organisms constantly integrate varying environmental signals to flexibly adapt their growth and development. Local fluctuations in water and nutrient availability, sudden changes in temperature or other abiotic and biotic stresses can trigger changes in the growth of plant organs. Multiple mutually interconnected hormonal signaling cascades act as essential endogenous translators of these exogenous signals in the adaptive responses of plants. Although the molecular backbones of hormone transduction pathways have been identified, the mechanisms underlying their interactions are largely unknown. Here, using genome wide transcriptome profiling we identify an auxin and cytokinin cross-talk component; SYNERGISTIC ON AUXIN AND CYTOKININ 1 (SYAC1), whose expression in roots is strictly dependent on both of these hormonal pathways. We show that SYAC1 is a regulator of secretory pathway, whose enhanced activity interferes with deposition of cell wall components and can fine-tune organ growth and sensitivity to soil pathogens.","lang":"eng"}],"pmid":1,"status":"public","has_accepted_license":"1","language":[{"iso":"eng"}],"quality_controlled":"1","doi":"10.1038/s41467-020-15895-5","oa_version":"Published Version","publisher":"Springer Nature","day":"01","department":[{"_id":"EvBe"}],"publication_identifier":{"eissn":["20411723"]},"tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"year":"2020","isi":1,"author":[{"last_name":"Hurny","orcid":"0000-0003-3638-1426","full_name":"Hurny, Andrej","id":"4DC4AF46-F248-11E8-B48F-1D18A9856A87","first_name":"Andrej"},{"orcid":"0000-0003-1923-2410","full_name":"Cuesta, Candela","last_name":"Cuesta","id":"33A3C818-F248-11E8-B48F-1D18A9856A87","first_name":"Candela"},{"last_name":"Cavallari","full_name":"Cavallari, Nicola","first_name":"Nicola","id":"457160E6-F248-11E8-B48F-1D18A9856A87"},{"id":"29B901B0-F248-11E8-B48F-1D18A9856A87","first_name":"Krisztina","orcid":"0000-0002-5503-4983","full_name":"Ötvös, Krisztina","last_name":"Ötvös"},{"full_name":"Duclercq, Jerome","last_name":"Duclercq","first_name":"Jerome"},{"last_name":"Dokládal","full_name":"Dokládal, Ladislav","first_name":"Ladislav"},{"full_name":"Montesinos López, Juan C","orcid":"0000-0001-9179-6099","last_name":"Montesinos López","id":"310A8E3E-F248-11E8-B48F-1D18A9856A87","first_name":"Juan C"},{"last_name":"Gallemi","orcid":"0000-0003-4675-6893","full_name":"Gallemi, Marçal","first_name":"Marçal","id":"460C6802-F248-11E8-B48F-1D18A9856A87"},{"id":"42FE702E-F248-11E8-B48F-1D18A9856A87","first_name":"Hana","last_name":"Semeradova","full_name":"Semeradova, Hana"},{"id":"A0385D1A-9376-11EA-A47D-9862C5E3AB22","first_name":"Thomas","last_name":"Rauter","full_name":"Rauter, Thomas"},{"first_name":"Irene","last_name":"Stenzel","full_name":"Stenzel, Irene"},{"first_name":"Geert","full_name":"Persiau, Geert","last_name":"Persiau"},{"first_name":"Freia","full_name":"Benade, Freia","last_name":"Benade"},{"first_name":"Rishikesh","full_name":"Bhalearo, Rishikesh","last_name":"Bhalearo"},{"first_name":"Eva","last_name":"Sýkorová","full_name":"Sýkorová, Eva"},{"first_name":"András","full_name":"Gorzsás, András","last_name":"Gorzsás"},{"full_name":"Sechet, Julien","last_name":"Sechet","first_name":"Julien"},{"full_name":"Mouille, Gregory","last_name":"Mouille","first_name":"Gregory"},{"first_name":"Ingo","last_name":"Heilmann","full_name":"Heilmann, Ingo"},{"full_name":"De Jaeger, Geert","last_name":"De Jaeger","first_name":"Geert"},{"first_name":"Jutta","full_name":"Ludwig-Müller, Jutta","last_name":"Ludwig-Müller"},{"first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8510-9739","full_name":"Benková, Eva","last_name":"Benková"}]},{"volume":71,"main_file_link":[{"open_access":"1","url":"https://hal.inrae.fr/hal-02619371"}],"issue":"15","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","intvolume":"        71","oa":1,"month":"07","title":"The Arabidopsis NRT1.1 transceptor coordinately controls auxin biosynthesis and transport to regulate root branching in response to nitrate","date_updated":"2023-08-21T07:07:30Z","date_published":"2020-07-25T00:00:00Z","article_processing_charge":"No","page":"4480-4494","publication":"Journal of Experimental Botany","date_created":"2020-06-08T10:10:28Z","external_id":{"isi":["000553127600013"],"pmid":["32428238"]},"_id":"7948","language":[{"iso":"eng"}],"status":"public","pmid":1,"abstract":[{"text":"In agricultural systems, nitrate is the main source of nitrogen available for plants. Besides its role as a nutrient, nitrate has been shown to act as a signal molecule for plant growth, development and stress responses. In Arabidopsis, the NRT1.1 nitrate transceptor represses lateral root (LR) development at low nitrate availability by promoting auxin basipetal transport out of the LR primordia (LRPs). In addition, our present study shows that NRT1.1 acts as a negative regulator of the TAR2 auxin biosynthetic gene expression in the root stele. This is expected to repress local auxin biosynthesis and thus to reduce acropetal auxin supply to the LRPs. Moreover, NRT1.1 also negatively affects expression of the LAX3 auxin influx carrier, thus preventing cell wall remodeling required for overlying tissues separation during LRP emergence. Both NRT1.1-mediated repression of TAR2 and LAX3 are suppressed at high nitrate availability, resulting in the nitrate induction of TAR2 and LAX3 expression that is required for optimal stimulation of LR development by nitrate. Altogether, our results indicate that the NRT1.1 transceptor coordinately controls several crucial auxin-associated processes required for LRP development, and as a consequence that NRT1.1 plays a much more integrated role than previously anticipated in regulating the nitrate response of root system architecture.","lang":"eng"}],"publication_status":"published","quality_controlled":"1","citation":{"apa":"Maghiaoui, A., Bouguyon, E., Cuesta, C., Perrine-Walker, F., Alcon, C., Krouk, G., … Bach, L. (2020). The Arabidopsis NRT1.1 transceptor coordinately controls auxin biosynthesis and transport to regulate root branching in response to nitrate. <i>Journal of Experimental Botany</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/jxb/eraa242\">https://doi.org/10.1093/jxb/eraa242</a>","short":"A. Maghiaoui, E. Bouguyon, C. Cuesta, F. Perrine-Walker, C. Alcon, G. Krouk, E. Benková, P. Nacry, A. Gojon, L. Bach, Journal of Experimental Botany 71 (2020) 4480–4494.","ieee":"A. Maghiaoui <i>et al.</i>, “The Arabidopsis NRT1.1 transceptor coordinately controls auxin biosynthesis and transport to regulate root branching in response to nitrate,” <i>Journal of Experimental Botany</i>, vol. 71, no. 15. Oxford University Press, pp. 4480–4494, 2020.","chicago":"Maghiaoui, A, E Bouguyon, Candela Cuesta, F Perrine-Walker, C Alcon, G Krouk, Eva Benková, P Nacry, A Gojon, and L Bach. “The Arabidopsis NRT1.1 Transceptor Coordinately Controls Auxin Biosynthesis and Transport to Regulate Root Branching in Response to Nitrate.” <i>Journal of Experimental Botany</i>. Oxford University Press, 2020. <a href=\"https://doi.org/10.1093/jxb/eraa242\">https://doi.org/10.1093/jxb/eraa242</a>.","ama":"Maghiaoui A, Bouguyon E, Cuesta C, et al. The Arabidopsis NRT1.1 transceptor coordinately controls auxin biosynthesis and transport to regulate root branching in response to nitrate. <i>Journal of Experimental Botany</i>. 2020;71(15):4480-4494. doi:<a href=\"https://doi.org/10.1093/jxb/eraa242\">10.1093/jxb/eraa242</a>","ista":"Maghiaoui A, Bouguyon E, Cuesta C, Perrine-Walker F, Alcon C, Krouk G, Benková E, Nacry P, Gojon A, Bach L. 2020. The Arabidopsis NRT1.1 transceptor coordinately controls auxin biosynthesis and transport to regulate root branching in response to nitrate. Journal of Experimental Botany. 71(15), 4480–4494.","mla":"Maghiaoui, A., et al. “The Arabidopsis NRT1.1 Transceptor Coordinately Controls Auxin Biosynthesis and Transport to Regulate Root Branching in Response to Nitrate.” <i>Journal of Experimental Botany</i>, vol. 71, no. 15, Oxford University Press, 2020, pp. 4480–94, doi:<a href=\"https://doi.org/10.1093/jxb/eraa242\">10.1093/jxb/eraa242</a>."},"type":"journal_article","article_type":"original","publication_identifier":{"issn":["0022-0957"],"eissn":["1460-2431"]},"department":[{"_id":"EvBe"}],"isi":1,"author":[{"last_name":"Maghiaoui","full_name":"Maghiaoui, A","first_name":"A"},{"first_name":"E","last_name":"Bouguyon","full_name":"Bouguyon, E"},{"id":"33A3C818-F248-11E8-B48F-1D18A9856A87","first_name":"Candela","orcid":"0000-0003-1923-2410","full_name":"Cuesta, Candela","last_name":"Cuesta"},{"last_name":"Perrine-Walker","full_name":"Perrine-Walker, F","first_name":"F"},{"first_name":"C","full_name":"Alcon, C","last_name":"Alcon"},{"full_name":"Krouk, G","last_name":"Krouk","first_name":"G"},{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","first_name":"Eva","last_name":"Benková","orcid":"0000-0002-8510-9739","full_name":"Benková, Eva"},{"last_name":"Nacry","full_name":"Nacry, P","first_name":"P"},{"full_name":"Gojon, A","last_name":"Gojon","first_name":"A"},{"first_name":"L","full_name":"Bach, L","last_name":"Bach"}],"year":"2020","oa_version":"Submitted Version","doi":"10.1093/jxb/eraa242","day":"25","publisher":"Oxford University Press"},{"year":"2020","isi":1,"author":[{"first_name":"Lukas","id":"2EEE7A2A-F248-11E8-B48F-1D18A9856A87","last_name":"Hörmayer","full_name":"Hörmayer, Lukas","orcid":"0000-0001-8295-2926"},{"id":"310A8E3E-F248-11E8-B48F-1D18A9856A87","first_name":"Juan C","orcid":"0000-0001-9179-6099","full_name":"Montesinos López, Juan C","last_name":"Montesinos López"},{"id":"44E59624-F248-11E8-B48F-1D18A9856A87","first_name":"Petra","last_name":"Marhavá","full_name":"Marhavá, Petra"},{"first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","full_name":"Benková, Eva","orcid":"0000-0002-8510-9739","last_name":"Benková"},{"id":"2E46069C-F248-11E8-B48F-1D18A9856A87","first_name":"Saiko","full_name":"Yoshida, Saiko","last_name":"Yoshida"},{"last_name":"Friml","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří"}],"publication_identifier":{"eissn":["1091-6490"],"issn":["0027-8424"]},"department":[{"_id":"JiFr"},{"_id":"EvBe"}],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png"},"publisher":"Proceedings of the National Academy of Sciences","day":"30","doi":"10.1073/pnas.2003346117","oa_version":"None","quality_controlled":"1","publication_status":"published","abstract":[{"lang":"eng","text":"Wound healing in plant tissues, consisting of rigid cell wall-encapsulated cells, represents a considerable challenge and occurs through largely unknown mechanisms distinct from those in animals. Owing to their inability to migrate, plant cells rely on targeted cell division and expansion to regenerate wounds. Strict coordination of these wound-induced responses is essential to ensure efficient, spatially restricted wound healing. Single-cell tracking by live imaging allowed us to gain mechanistic insight into the wound perception and coordination of wound responses after laser-based wounding in Arabidopsis root. We revealed a crucial contribution of the collapse of damaged cells in wound perception and detected an auxin increase specific to cells immediately adjacent to the wound. This localized auxin increase balances wound-induced cell expansion and restorative division rates in a dose-dependent manner, leading to tumorous overproliferation when the canonical TIR1 auxin signaling is disrupted. Auxin and wound-induced turgor pressure changes together also spatially define the activation of key components of regeneration, such as the transcription regulator ERF115. Our observations suggest that the wound signaling involves the sensing of collapse of damaged cells and a local auxin signaling activation to coordinate the downstream transcriptional responses in the immediate wound vicinity."}],"pmid":1,"status":"public","has_accepted_license":"1","language":[{"iso":"eng"}],"ddc":["580"],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"article_type":"original","file":[{"file_name":"2020_PNAS_Hoermayer.pdf","access_level":"open_access","checksum":"908b09437680181de9990915f2113aca","relation":"main_file","date_updated":"2020-07-14T12:48:07Z","content_type":"application/pdf","file_size":2407102,"creator":"dernst","date_created":"2020-06-23T11:30:53Z","file_id":"8009"}],"type":"journal_article","citation":{"apa":"Hörmayer, L., Montesinos López, J. C., Marhavá, P., Benková, E., Yoshida, S., &#38; Friml, J. (2020). Wounding-induced changes in cellular pressure and localized auxin signalling spatially coordinate restorative divisions in roots. <i>Proceedings of the National Academy of Sciences</i>. Proceedings of the National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.2003346117\">https://doi.org/10.1073/pnas.2003346117</a>","short":"L. Hörmayer, J.C. Montesinos López, P. Marhavá, E. Benková, S. Yoshida, J. Friml, Proceedings of the National Academy of Sciences 117 (2020).","ieee":"L. Hörmayer, J. C. Montesinos López, P. Marhavá, E. Benková, S. Yoshida, and J. Friml, “Wounding-induced changes in cellular pressure and localized auxin signalling spatially coordinate restorative divisions in roots,” <i>Proceedings of the National Academy of Sciences</i>, vol. 117, no. 26. Proceedings of the National Academy of Sciences, 2020.","ama":"Hörmayer L, Montesinos López JC, Marhavá P, Benková E, Yoshida S, Friml J. Wounding-induced changes in cellular pressure and localized auxin signalling spatially coordinate restorative divisions in roots. <i>Proceedings of the National Academy of Sciences</i>. 2020;117(26). doi:<a href=\"https://doi.org/10.1073/pnas.2003346117\">10.1073/pnas.2003346117</a>","chicago":"Hörmayer, Lukas, Juan C Montesinos López, Petra Marhavá, Eva Benková, Saiko Yoshida, and Jiří Friml. “Wounding-Induced Changes in Cellular Pressure and Localized Auxin Signalling Spatially Coordinate Restorative Divisions in Roots.” <i>Proceedings of the National Academy of Sciences</i>. Proceedings of the National Academy of Sciences, 2020. <a href=\"https://doi.org/10.1073/pnas.2003346117\">https://doi.org/10.1073/pnas.2003346117</a>.","ista":"Hörmayer L, Montesinos López JC, Marhavá P, Benková E, Yoshida S, Friml J. 2020. Wounding-induced changes in cellular pressure and localized auxin signalling spatially coordinate restorative divisions in roots. Proceedings of the National Academy of Sciences. 117(26), 202003346.","mla":"Hörmayer, Lukas, et al. “Wounding-Induced Changes in Cellular Pressure and Localized Auxin Signalling Spatially Coordinate Restorative Divisions in Roots.” <i>Proceedings of the National Academy of Sciences</i>, vol. 117, no. 26, 202003346, Proceedings of the National Academy of Sciences, 2020, doi:<a href=\"https://doi.org/10.1073/pnas.2003346117\">10.1073/pnas.2003346117</a>."},"article_processing_charge":"No","date_published":"2020-06-30T00:00:00Z","ec_funded":1,"date_updated":"2024-03-25T23:30:06Z","title":"Wounding-induced changes in cellular pressure and localized auxin signalling spatially coordinate restorative divisions in roots","month":"06","_id":"8002","project":[{"_id":"261099A6-B435-11E9-9278-68D0E5697425","grant_number":"742985","call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants"},{"name":"RNA-directed DNA methylation in plant development","grant_number":"P29988","_id":"262EF96E-B435-11E9-9278-68D0E5697425","call_identifier":"FWF"}],"external_id":{"pmid":["32541049"],"isi":["000565729700033"]},"date_created":"2020-06-22T13:33:52Z","publication":"Proceedings of the National Academy of Sciences","file_date_updated":"2020-07-14T12:48:07Z","article_number":"202003346","intvolume":"       117","oa":1,"issue":"26","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","related_material":{"record":[{"relation":"dissertation_contains","status":"public","id":"9992"}],"link":[{"relation":"press_release","description":"News on IST Homepage","url":"https://ist.ac.at/en/news/how-wounded-plants-coordinate-their-healing/"}]},"volume":117,"license":"https://creativecommons.org/licenses/by-nc-nd/4.0/","scopus_import":"1"},{"file":[{"creator":"dernst","date_created":"2020-12-02T09:13:23Z","file_id":"8827","success":1,"access_level":"open_access","file_name":"2020_EMBO_Montesinos.pdf","content_type":"application/pdf","date_updated":"2020-12-02T09:13:23Z","file_size":3497156,"relation":"main_file","checksum":"43d2b36598708e6ab05c69074e191d57"}],"type":"journal_article","citation":{"ieee":"J. C. Montesinos López <i>et al.</i>, “Phytohormone cytokinin guides microtubule dynamics during cell progression from proliferative to differentiated stage,” <i>The Embo Journal</i>, vol. 39, no. 17. Embo Press, 2020.","short":"J.C. Montesinos López, A. Abuzeineh, A. Kopf, A. Juanes Garcia, K. Ötvös, J. Petrášek, M.K. Sixt, E. Benková, The Embo Journal 39 (2020).","apa":"Montesinos López, J. C., Abuzeineh, A., Kopf, A., Juanes Garcia, A., Ötvös, K., Petrášek, J., … Benková, E. (2020). Phytohormone cytokinin guides microtubule dynamics during cell progression from proliferative to differentiated stage. <i>The Embo Journal</i>. Embo Press. <a href=\"https://doi.org/10.15252/embj.2019104238\">https://doi.org/10.15252/embj.2019104238</a>","mla":"Montesinos López, Juan C., et al. “Phytohormone Cytokinin Guides Microtubule Dynamics during Cell Progression from Proliferative to Differentiated Stage.” <i>The Embo Journal</i>, vol. 39, no. 17, e104238, Embo Press, 2020, doi:<a href=\"https://doi.org/10.15252/embj.2019104238\">10.15252/embj.2019104238</a>.","ista":"Montesinos López JC, Abuzeineh A, Kopf A, Juanes Garcia A, Ötvös K, Petrášek J, Sixt MK, Benková E. 2020. Phytohormone cytokinin guides microtubule dynamics during cell progression from proliferative to differentiated stage. The Embo Journal. 39(17), e104238.","ama":"Montesinos López JC, Abuzeineh A, Kopf A, et al. Phytohormone cytokinin guides microtubule dynamics during cell progression from proliferative to differentiated stage. <i>The Embo Journal</i>. 2020;39(17). doi:<a href=\"https://doi.org/10.15252/embj.2019104238\">10.15252/embj.2019104238</a>","chicago":"Montesinos López, Juan C, A Abuzeineh, Aglaja Kopf, Alba Juanes Garcia, Krisztina Ötvös, J Petrášek, Michael K Sixt, and Eva Benková. “Phytohormone Cytokinin Guides Microtubule Dynamics during Cell Progression from Proliferative to Differentiated Stage.” <i>The Embo Journal</i>. Embo Press, 2020. <a href=\"https://doi.org/10.15252/embj.2019104238\">https://doi.org/10.15252/embj.2019104238</a>."},"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"ddc":["580"],"article_type":"original","publication_status":"published","abstract":[{"lang":"eng","text":"Cell production and differentiation for the acquisition of specific functions are key features of living systems. The dynamic network of cellular microtubules provides the necessary platform to accommodate processes associated with the transition of cells through the individual phases of cytogenesis. Here, we show that the plant hormone cytokinin fine‐tunes the activity of the microtubular cytoskeleton during cell differentiation and counteracts microtubular rearrangements driven by the hormone auxin. The endogenous upward gradient of cytokinin activity along the longitudinal growth axis in Arabidopsis thaliana roots correlates with robust rearrangements of the microtubule cytoskeleton in epidermal cells progressing from the proliferative to the differentiation stage. Controlled increases in cytokinin activity result in premature re‐organization of the microtubule network from transversal to an oblique disposition in cells prior to their differentiation, whereas attenuated hormone perception delays cytoskeleton conversion into a configuration typical for differentiated cells. Intriguingly, cytokinin can interfere with microtubules also in animal cells, such as leukocytes, suggesting that a cytokinin‐sensitive control pathway for the microtubular cytoskeleton may be at least partially conserved between plant and animal cells."}],"language":[{"iso":"eng"}],"status":"public","pmid":1,"has_accepted_license":"1","quality_controlled":"1","doi":"10.15252/embj.2019104238","oa_version":"Published Version","publisher":"Embo Press","day":"01","department":[{"_id":"MiSi"},{"_id":"EvBe"}],"publication_identifier":{"eissn":["1460-2075"],"issn":["0261-4189"]},"tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"author":[{"full_name":"Montesinos López, Juan C","orcid":"0000-0001-9179-6099","last_name":"Montesinos López","first_name":"Juan C","id":"310A8E3E-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Abuzeineh","full_name":"Abuzeineh, A","first_name":"A"},{"id":"31DAC7B6-F248-11E8-B48F-1D18A9856A87","first_name":"Aglaja","orcid":"0000-0002-2187-6656","full_name":"Kopf, Aglaja","last_name":"Kopf"},{"id":"40F05888-F248-11E8-B48F-1D18A9856A87","first_name":"Alba","orcid":"0000-0002-1009-9652","full_name":"Juanes Garcia, Alba","last_name":"Juanes Garcia"},{"id":"29B901B0-F248-11E8-B48F-1D18A9856A87","first_name":"Krisztina","full_name":"Ötvös, Krisztina","orcid":"0000-0002-5503-4983","last_name":"Ötvös"},{"first_name":"J","full_name":"Petrášek, J","last_name":"Petrášek"},{"first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K","last_name":"Sixt"},{"first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","last_name":"Benková","full_name":"Benková, Eva","orcid":"0000-0002-8510-9739"}],"isi":1,"year":"2020","scopus_import":"1","volume":39,"article_number":"e104238","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","acknowledgement":"We thank Takashi Aoyama, David Alabadi, and Bert De Rybel for sharing material, Jiří Friml, Maciek Adamowski, and Katerina Schwarzerová for inspiring discussions, and Martine De Cock for help in preparing the manuscript. This research was supported by the Scientific Service Units (SSUs) of IST Austria through resources provided by the Bioimaging Facility (BIF), especially to Robert Hauschild; and the Life Science Facility (LSF). J.C.M. is the recipient of a EMBO Long‐Term Fellowship (ALTF number 710‐2016). This work was supported with MEYS CR, project no.CZ.02.1.01/0.0/0.0/16_019/0000738 to J.P., and by the Austrian Science Fund (FWF01_I1774S) to E.B.","issue":"17","oa":1,"intvolume":"        39","file_date_updated":"2020-12-02T09:13:23Z","publication":"The Embo Journal","project":[{"name":"Molecular mechanism of auxindriven formative divisions delineating lateral root organogenesis in plants","grant_number":"ALTF710-2016","_id":"253E54C8-B435-11E9-9278-68D0E5697425"},{"name":"Hormone cross-talk drives nutrient dependent plant development","call_identifier":"FWF","_id":"2542D156-B435-11E9-9278-68D0E5697425","grant_number":"I 1774-B16"}],"_id":"8142","date_created":"2020-07-21T09:08:38Z","external_id":{"isi":["000548311800001"],"pmid":["32667089"]},"date_updated":"2023-09-05T13:05:47Z","title":"Phytohormone cytokinin guides microtubule dynamics during cell progression from proliferative to differentiated stage","month":"09","date_published":"2020-09-01T00:00:00Z","article_processing_charge":"Yes (via OA deal)"},{"volume":13,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","issue":"5","intvolume":"        13","oa":1,"page":"717-731","file_date_updated":"2024-02-28T12:39:56Z","publication":"Molecular Plant","date_created":"2024-02-28T08:55:56Z","external_id":{"pmid":["32087370"]},"_id":"15037","month":"05","title":"TOLs function as ubiquitin receptors in the early steps of the ESCRT pathway in higher plants","date_updated":"2024-02-28T12:41:52Z","date_published":"2020-05-04T00:00:00Z","article_processing_charge":"No","keyword":["Plant Science","Molecular Biology"],"type":"journal_article","citation":{"mla":"Moulinier-Anzola, Jeanette, et al. “TOLs Function as Ubiquitin Receptors in the Early Steps of the ESCRT Pathway in Higher Plants.” <i>Molecular Plant</i>, vol. 13, no. 5, Elsevier, 2020, pp. 717–31, doi:<a href=\"https://doi.org/10.1016/j.molp.2020.02.012\">10.1016/j.molp.2020.02.012</a>.","ista":"Moulinier-Anzola J, Schwihla M, De-Araújo L, Artner C, Jörg L, Konstantinova N, Luschnig C, Korbei B. 2020. TOLs function as ubiquitin receptors in the early steps of the ESCRT pathway in higher plants. Molecular Plant. 13(5), 717–731.","ama":"Moulinier-Anzola J, Schwihla M, De-Araújo L, et al. TOLs function as ubiquitin receptors in the early steps of the ESCRT pathway in higher plants. <i>Molecular Plant</i>. 2020;13(5):717-731. doi:<a href=\"https://doi.org/10.1016/j.molp.2020.02.012\">10.1016/j.molp.2020.02.012</a>","chicago":"Moulinier-Anzola, Jeanette, Maximilian Schwihla, Lucinda De-Araújo, Christina Artner, Lisa Jörg, Nataliia Konstantinova, Christian Luschnig, and Barbara Korbei. “TOLs Function as Ubiquitin Receptors in the Early Steps of the ESCRT Pathway in Higher Plants.” <i>Molecular Plant</i>. Elsevier, 2020. <a href=\"https://doi.org/10.1016/j.molp.2020.02.012\">https://doi.org/10.1016/j.molp.2020.02.012</a>.","ieee":"J. Moulinier-Anzola <i>et al.</i>, “TOLs function as ubiquitin receptors in the early steps of the ESCRT pathway in higher plants,” <i>Molecular Plant</i>, vol. 13, no. 5. Elsevier, pp. 717–731, 2020.","apa":"Moulinier-Anzola, J., Schwihla, M., De-Araújo, L., Artner, C., Jörg, L., Konstantinova, N., … Korbei, B. (2020). TOLs function as ubiquitin receptors in the early steps of the ESCRT pathway in higher plants. <i>Molecular Plant</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.molp.2020.02.012\">https://doi.org/10.1016/j.molp.2020.02.012</a>","short":"J. Moulinier-Anzola, M. Schwihla, L. De-Araújo, C. Artner, L. Jörg, N. Konstantinova, C. Luschnig, B. Korbei, Molecular Plant 13 (2020) 717–731."},"file":[{"checksum":"c538a5008f7827f62d17d40a3bfabe65","relation":"main_file","file_size":3089212,"content_type":"application/pdf","date_updated":"2024-02-28T12:39:56Z","file_name":"2020_MolecularPlant_MoulinierAnzola.pdf","access_level":"open_access","success":1,"file_id":"15038","date_created":"2024-02-28T12:39:56Z","creator":"dernst"}],"article_type":"original","ddc":["580"],"language":[{"iso":"eng"}],"status":"public","has_accepted_license":"1","pmid":1,"publication_status":"published","abstract":[{"text":"Protein abundance and localization at the plasma membrane (PM) shapes plant development and mediates adaptation to changing environmental conditions. It is regulated by ubiquitination, a post-translational modification crucial for the proper sorting of endocytosed PM proteins to the vacuole for subsequent degradation. To understand the significance and the variety of roles played by this reversible modification, the function of ubiquitin receptors, which translate the ubiquitin signature into a cellular response, needs to be elucidated. In this study, we show that TOL (TOM1-like) proteins function in plants as multivalent ubiquitin receptors, governing ubiquitinated cargo delivery to the vacuole via the conserved Endosomal Sorting Complex Required for Transport (ESCRT) pathway. TOL2 and TOL6 interact with components of the ESCRT machinery and bind to K63-linked ubiquitin via two tandemly arranged conserved ubiquitin-binding domains. Mutation of these domains results not only in a loss of ubiquitin binding but also altered localization, abolishing TOL6 ubiquitin receptor activity. Function and localization of TOL6 is itself regulated by ubiquitination, whereby TOL6 ubiquitination potentially modulates degradation of PM-localized cargoes, assisting in the fine-tuning of the delicate interplay between protein recycling and downregulation. Taken together, our findings demonstrate the function and regulation of a ubiquitin receptor that mediates vacuolar degradation of PM proteins in higher plants.","lang":"eng"}],"quality_controlled":"1","oa_version":"Published Version","doi":"10.1016/j.molp.2020.02.012","day":"04","publisher":"Elsevier","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"publication_identifier":{"issn":["1674-2052"]},"department":[{"_id":"EvBe"}],"author":[{"first_name":"Jeanette","last_name":"Moulinier-Anzola","full_name":"Moulinier-Anzola, Jeanette"},{"first_name":"Maximilian","full_name":"Schwihla, Maximilian","last_name":"Schwihla"},{"full_name":"De-Araújo, Lucinda","last_name":"De-Araújo","first_name":"Lucinda"},{"full_name":"Artner, Christina","last_name":"Artner","id":"45DF286A-F248-11E8-B48F-1D18A9856A87","first_name":"Christina"},{"first_name":"Lisa","full_name":"Jörg, Lisa","last_name":"Jörg"},{"full_name":"Konstantinova, Nataliia","last_name":"Konstantinova","first_name":"Nataliia"},{"last_name":"Luschnig","full_name":"Luschnig, Christian","first_name":"Christian"},{"first_name":"Barbara","full_name":"Korbei, Barbara","last_name":"Korbei"}],"year":"2020"},{"publication":"Molecular Plant","page":"1312-1314","project":[{"_id":"2685A872-B435-11E9-9278-68D0E5697425","name":"Hormonal regulation of plant adaptive responses to environmental signals"}],"_id":"6920","date_created":"2019-09-30T10:00:40Z","external_id":{"isi":["000489132500002"],"pmid":["31541740"]},"date_updated":"2023-08-30T06:55:02Z","title":"Ethylene and cytokinin - partners in root growth regulation","month":"10","date_published":"2019-10-07T00:00:00Z","article_processing_charge":"No","scopus_import":"1","volume":12,"issue":"10","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","intvolume":"        12","doi":"10.1016/j.molp.2019.09.003","oa_version":"None","publisher":"Cell Press","day":"07","department":[{"_id":"EvBe"}],"publication_identifier":{"issn":["1674-2052","1752-9867"]},"isi":1,"author":[{"first_name":"Christina","id":"45DF286A-F248-11E8-B48F-1D18A9856A87","last_name":"Artner","full_name":"Artner, Christina"},{"full_name":"Benková, Eva","orcid":"0000-0002-8510-9739","last_name":"Benková","first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87"}],"year":"2019","citation":{"chicago":"Artner, Christina, and Eva Benková. “Ethylene and Cytokinin - Partners in Root Growth Regulation.” <i>Molecular Plant</i>. Cell Press, 2019. <a href=\"https://doi.org/10.1016/j.molp.2019.09.003\">https://doi.org/10.1016/j.molp.2019.09.003</a>.","ama":"Artner C, Benková E. Ethylene and cytokinin - partners in root growth regulation. <i>Molecular Plant</i>. 2019;12(10):1312-1314. doi:<a href=\"https://doi.org/10.1016/j.molp.2019.09.003\">10.1016/j.molp.2019.09.003</a>","mla":"Artner, Christina, and Eva Benková. “Ethylene and Cytokinin - Partners in Root Growth Regulation.” <i>Molecular Plant</i>, vol. 12, no. 10, Cell Press, 2019, pp. 1312–14, doi:<a href=\"https://doi.org/10.1016/j.molp.2019.09.003\">10.1016/j.molp.2019.09.003</a>.","ista":"Artner C, Benková E. 2019. Ethylene and cytokinin - partners in root growth regulation. Molecular Plant. 12(10), 1312–1314.","short":"C. Artner, E. Benková, Molecular Plant 12 (2019) 1312–1314.","apa":"Artner, C., &#38; Benková, E. (2019). Ethylene and cytokinin - partners in root growth regulation. <i>Molecular Plant</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.molp.2019.09.003\">https://doi.org/10.1016/j.molp.2019.09.003</a>","ieee":"C. Artner and E. Benková, “Ethylene and cytokinin - partners in root growth regulation,” <i>Molecular Plant</i>, vol. 12, no. 10. Cell Press, pp. 1312–1314, 2019."},"type":"journal_article","article_type":"original","publication_status":"published","language":[{"iso":"eng"}],"pmid":1,"status":"public","quality_controlled":"1"},{"article_processing_charge":"No","date_published":"2019-12-01T00:00:00Z","month":"12","title":"Editorial overview: Cell biology in the era of omics?","date_updated":"2023-09-07T14:56:55Z","external_id":{"isi":["000502890600001"],"pmid":["31787165"]},"date_created":"2020-01-29T16:00:07Z","_id":"7394","page":"A1-A2","publication":"Current Opinion in Plant Biology","intvolume":"        52","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","issue":"12","volume":52,"scopus_import":"1","year":"2019","author":[{"first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","full_name":"Benková, Eva","orcid":"0000-0002-8510-9739","last_name":"Benková"},{"full_name":"Dagdas, Yasin","last_name":"Dagdas","first_name":"Yasin"}],"isi":1,"department":[{"_id":"EvBe"}],"publication_identifier":{"issn":["1369-5266"]},"day":"01","publisher":"Elsevier","oa_version":"None","doi":"10.1016/j.pbi.2019.11.002","quality_controlled":"1","status":"public","pmid":1,"language":[{"iso":"eng"}],"publication_status":"published","article_type":"letter_note","citation":{"mla":"Benková, Eva, and Yasin Dagdas. “Editorial Overview: Cell Biology in the Era of Omics?” <i>Current Opinion in Plant Biology</i>, vol. 52, no. 12, Elsevier, 2019, pp. A1–2, doi:<a href=\"https://doi.org/10.1016/j.pbi.2019.11.002\">10.1016/j.pbi.2019.11.002</a>.","ista":"Benková E, Dagdas Y. 2019. Editorial overview: Cell biology in the era of omics? Current Opinion in Plant Biology. 52(12), A1–A2.","ama":"Benková E, Dagdas Y. Editorial overview: Cell biology in the era of omics? <i>Current Opinion in Plant Biology</i>. 2019;52(12):A1-A2. doi:<a href=\"https://doi.org/10.1016/j.pbi.2019.11.002\">10.1016/j.pbi.2019.11.002</a>","chicago":"Benková, Eva, and Yasin Dagdas. “Editorial Overview: Cell Biology in the Era of Omics?” <i>Current Opinion in Plant Biology</i>. Elsevier, 2019. <a href=\"https://doi.org/10.1016/j.pbi.2019.11.002\">https://doi.org/10.1016/j.pbi.2019.11.002</a>.","ieee":"E. Benková and Y. Dagdas, “Editorial overview: Cell biology in the era of omics?,” <i>Current Opinion in Plant Biology</i>, vol. 52, no. 12. Elsevier, pp. A1–A2, 2019.","short":"E. Benková, Y. Dagdas, Current Opinion in Plant Biology 52 (2019) A1–A2.","apa":"Benková, E., &#38; Dagdas, Y. (2019). Editorial overview: Cell biology in the era of omics? <i>Current Opinion in Plant Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.pbi.2019.11.002\">https://doi.org/10.1016/j.pbi.2019.11.002</a>"},"type":"journal_article"},{"title":"A SOSEKI-based coordinate system interprets global polarity cues in arabidopsis","date_updated":"2023-08-24T14:46:47Z","month":"02","article_processing_charge":"No","date_published":"2019-02-08T00:00:00Z","ec_funded":1,"publication":"Nature Plants","page":"160-166","_id":"6023","project":[{"name":"International IST Postdoc Fellowship Programme","grant_number":"291734","_id":"25681D80-B435-11E9-9278-68D0E5697425","call_identifier":"FP7"}],"external_id":{"isi":["000460479600014"]},"date_created":"2019-02-17T22:59:21Z","main_file_link":[{"url":"https://www.biorxiv.org/content/10.1101/479113v1.abstract","open_access":"1"}],"volume":5,"intvolume":"         5","oa":1,"issue":"2","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","scopus_import":"1","department":[{"_id":"JiFr"},{"_id":"EvBe"}],"year":"2019","isi":1,"author":[{"full_name":"Yoshida, Saiko","last_name":"Yoshida","id":"2E46069C-F248-11E8-B48F-1D18A9856A87","first_name":"Saiko"},{"first_name":"Alja","full_name":"Van Der Schuren, Alja","last_name":"Van Der Schuren"},{"first_name":"Maritza","last_name":"Van Dop","full_name":"Van Dop, Maritza"},{"full_name":"Van Galen, Luc","last_name":"Van Galen","first_name":"Luc"},{"first_name":"Shunsuke","last_name":"Saiga","full_name":"Saiga, Shunsuke"},{"full_name":"Adibi, Milad","last_name":"Adibi","first_name":"Milad"},{"full_name":"Möller, Barbara","last_name":"Möller","first_name":"Barbara"},{"last_name":"Ten Hove","full_name":"Ten Hove, Colette A.","first_name":"Colette A."},{"full_name":"Marhavy, Peter","orcid":"0000-0001-5227-5741","last_name":"Marhavy","first_name":"Peter","id":"3F45B078-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Richard","full_name":"Smith, Richard","last_name":"Smith"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","last_name":"Friml"},{"first_name":"Dolf","full_name":"Weijers, Dolf","last_name":"Weijers"}],"doi":"10.1038/s41477-019-0363-6","oa_version":"Submitted Version","publisher":"Springer Nature","day":"08","publication_status":"published","abstract":[{"lang":"eng","text":"Multicellular development requires coordinated cell polarization relative to body axes, and translation to oriented cell division 1–3 . In plants, it is unknown how cell polarities are connected to organismal axes and translated to division. Here, we identify Arabidopsis SOSEKI proteins that integrate apical–basal and radial organismal axes to localize to polar cell edges. Localization does not depend on tissue context, requires cell wall integrity and is defined by a transferrable, protein-specific motif. A Domain of Unknown Function in SOSEKI proteins resembles the DIX oligomerization domain in the animal Dishevelled polarity regulator. The DIX-like domain self-interacts and is required for edge localization and for influencing division orientation, together with a second domain that defines the polar membrane domain. Our work shows that SOSEKI proteins locally interpret global polarity cues and can influence cell division orientation. Furthermore, this work reveals that, despite fundamental differences, cell polarity mechanisms in plants and animals converge on a similar protein domain."}],"status":"public","language":[{"iso":"eng"}],"quality_controlled":"1","type":"journal_article","citation":{"ama":"Yoshida S, Van Der Schuren A, Van Dop M, et al. A SOSEKI-based coordinate system interprets global polarity cues in arabidopsis. <i>Nature Plants</i>. 2019;5(2):160-166. doi:<a href=\"https://doi.org/10.1038/s41477-019-0363-6\">10.1038/s41477-019-0363-6</a>","chicago":"Yoshida, Saiko, Alja Van Der Schuren, Maritza Van Dop, Luc Van Galen, Shunsuke Saiga, Milad Adibi, Barbara Möller, et al. “A SOSEKI-Based Coordinate System Interprets Global Polarity Cues in Arabidopsis.” <i>Nature Plants</i>. Springer Nature, 2019. <a href=\"https://doi.org/10.1038/s41477-019-0363-6\">https://doi.org/10.1038/s41477-019-0363-6</a>.","mla":"Yoshida, Saiko, et al. “A SOSEKI-Based Coordinate System Interprets Global Polarity Cues in Arabidopsis.” <i>Nature Plants</i>, vol. 5, no. 2, Springer Nature, 2019, pp. 160–66, doi:<a href=\"https://doi.org/10.1038/s41477-019-0363-6\">10.1038/s41477-019-0363-6</a>.","ista":"Yoshida S, Van Der Schuren A, Van Dop M, Van Galen L, Saiga S, Adibi M, Möller B, Ten Hove CA, Marhavý P, Smith R, Friml J, Weijers D. 2019. A SOSEKI-based coordinate system interprets global polarity cues in arabidopsis. Nature Plants. 5(2), 160–166.","short":"S. Yoshida, A. Van Der Schuren, M. Van Dop, L. Van Galen, S. Saiga, M. Adibi, B. Möller, C.A. Ten Hove, P. Marhavý, R. Smith, J. Friml, D. Weijers, Nature Plants 5 (2019) 160–166.","apa":"Yoshida, S., Van Der Schuren, A., Van Dop, M., Van Galen, L., Saiga, S., Adibi, M., … Weijers, D. (2019). A SOSEKI-based coordinate system interprets global polarity cues in arabidopsis. <i>Nature Plants</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41477-019-0363-6\">https://doi.org/10.1038/s41477-019-0363-6</a>","ieee":"S. Yoshida <i>et al.</i>, “A SOSEKI-based coordinate system interprets global polarity cues in arabidopsis,” <i>Nature Plants</i>, vol. 5, no. 2. Springer Nature, pp. 160–166, 2019."}},{"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","issue":"4","oa":1,"intvolume":"       177","volume":177,"related_material":{"link":[{"relation":"press_release","description":"News on IST Homepage","url":"https://ist.ac.at/en/news/specialized-plant-cells-regain-stem-cell-features-to-heal-wounds/"}],"record":[{"id":"9992","relation":"dissertation_contains","status":"public"}]},"scopus_import":"1","date_published":"2019-05-02T00:00:00Z","article_processing_charge":"No","ec_funded":1,"title":"Re-activation of stem cell pathways for pattern restoration in plant wound healing","date_updated":"2024-03-25T23:30:06Z","month":"05","project":[{"call_identifier":"H2020","_id":"261099A6-B435-11E9-9278-68D0E5697425","grant_number":"742985","name":"Tracing Evolution of Auxin Transport and Polarity in Plants"}],"_id":"6351","date_created":"2019-04-28T21:59:14Z","external_id":{"pmid":["31051107"],"isi":["000466843000015"]},"file_date_updated":"2020-07-14T12:47:28Z","publication":"Cell","page":"957-969.e13","quality_controlled":"1","publication_status":"published","abstract":[{"lang":"eng","text":"A process of restorative patterning in plant roots correctly replaces eliminated cells to heal local injuries despite the absence of cell migration, which underpins wound healing in animals. \r\n\r\nPatterning in plants relies on oriented cell divisions and acquisition of specific cell identities. Plants regularly endure wounds caused by abiotic or biotic environmental stimuli and have developed extraordinary abilities to restore their tissues after injuries. Here, we provide insight into a mechanism of restorative patterning that repairs tissues after wounding. Laser-assisted elimination of different cells in Arabidopsis root combined with live-imaging tracking during vertical growth allowed analysis of the regeneration processes in vivo. Specifically, the cells adjacent to the inner side of the injury re-activated their stem cell transcriptional programs. They accelerated their progression through cell cycle, coordinately changed the cell division orientation, and ultimately acquired de novo the correct cell fates to replace missing cells. These observations highlight existence of unknown intercellular positional signaling and demonstrate the capability of specified cells to re-acquire stem cell programs as a crucial part of the plant-specific mechanism of wound healing."}],"language":[{"iso":"eng"}],"pmid":1,"has_accepted_license":"1","status":"public","ddc":["570"],"acknowledged_ssus":[{"_id":"Bio"}],"file":[{"date_created":"2019-05-13T06:12:45Z","creator":"dernst","file_id":"6411","file_name":"2019_Cell_Marhava.pdf","access_level":"open_access","checksum":"4ceba04a96a74f5092ec3ce2c579a0c7","relation":"main_file","content_type":"application/pdf","date_updated":"2020-07-14T12:47:28Z","file_size":10272032}],"citation":{"chicago":"Marhavá, Petra, Lukas Hörmayer, Saiko Yoshida, Peter Marhavý, Eva Benková, and Jiří Friml. “Re-Activation of Stem Cell Pathways for Pattern Restoration in Plant Wound Healing.” <i>Cell</i>. Elsevier, 2019. <a href=\"https://doi.org/10.1016/j.cell.2019.04.015\">https://doi.org/10.1016/j.cell.2019.04.015</a>.","ama":"Marhavá P, Hörmayer L, Yoshida S, Marhavý P, Benková E, Friml J. Re-activation of stem cell pathways for pattern restoration in plant wound healing. <i>Cell</i>. 2019;177(4):957-969.e13. doi:<a href=\"https://doi.org/10.1016/j.cell.2019.04.015\">10.1016/j.cell.2019.04.015</a>","mla":"Marhavá, Petra, et al. “Re-Activation of Stem Cell Pathways for Pattern Restoration in Plant Wound Healing.” <i>Cell</i>, vol. 177, no. 4, Elsevier, 2019, p. 957–969.e13, doi:<a href=\"https://doi.org/10.1016/j.cell.2019.04.015\">10.1016/j.cell.2019.04.015</a>.","ista":"Marhavá P, Hörmayer L, Yoshida S, Marhavý P, Benková E, Friml J. 2019. Re-activation of stem cell pathways for pattern restoration in plant wound healing. Cell. 177(4), 957–969.e13.","apa":"Marhavá, P., Hörmayer, L., Yoshida, S., Marhavý, P., Benková, E., &#38; Friml, J. (2019). Re-activation of stem cell pathways for pattern restoration in plant wound healing. <i>Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cell.2019.04.015\">https://doi.org/10.1016/j.cell.2019.04.015</a>","short":"P. Marhavá, L. Hörmayer, S. Yoshida, P. Marhavý, E. Benková, J. Friml, Cell 177 (2019) 957–969.e13.","ieee":"P. Marhavá, L. Hörmayer, S. Yoshida, P. Marhavý, E. Benková, and J. Friml, “Re-activation of stem cell pathways for pattern restoration in plant wound healing,” <i>Cell</i>, vol. 177, no. 4. Elsevier, p. 957–969.e13, 2019."},"type":"journal_article","isi":1,"author":[{"full_name":"Marhavá, Petra","last_name":"Marhavá","first_name":"Petra","id":"44E59624-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Hörmayer","full_name":"Hörmayer, Lukas","orcid":"0000-0001-8295-2926","first_name":"Lukas","id":"2EEE7A2A-F248-11E8-B48F-1D18A9856A87"},{"id":"2E46069C-F248-11E8-B48F-1D18A9856A87","first_name":"Saiko","last_name":"Yoshida","full_name":"Yoshida, Saiko"},{"first_name":"Peter","id":"3F45B078-F248-11E8-B48F-1D18A9856A87","last_name":"Marhavy","full_name":"Marhavy, Peter","orcid":"0000-0001-5227-5741"},{"first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8510-9739","full_name":"Benková, Eva","last_name":"Benková"},{"last_name":"Friml","orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"year":"2019","publication_identifier":{"eissn":["10974172"],"issn":["00928674"]},"department":[{"_id":"JiFr"},{"_id":"EvBe"}],"tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"publisher":"Elsevier","day":"02","doi":"10.1016/j.cell.2019.04.015","oa_version":"Published Version"},{"scopus_import":"1","article_number":"dev175919","oa":1,"intvolume":"       146","issue":"17","acknowledgement":"We thank Jiri Friml and Phillip Brewer for inspiring discussion and for help in preparing the manuscript. This research was supported by the Scientific Service Units (SSU) of IST-Austria through resources provided by the Bioimaging Facility\r\n(BIF), the Life Science Facility (LSF).\r\nThis work was supported by grants from the European Research Council (Starting Independent Research Grant ERC-2007-Stg- 207362-HCPO to E.B.). J.P. and M.S. received funds from European Regional Development Fund-Project ‘Centre for Experimental Plant Biology’ (No. CZ.02.1.01/0.0/0.0/16_019/0000738).","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1242/dev.175919"}],"volume":146,"project":[{"name":"Hormonal cross-talk in plant organogenesis","call_identifier":"FP7","_id":"253FCA6A-B435-11E9-9278-68D0E5697425","grant_number":"207362"}],"_id":"6897","external_id":{"pmid":["31391194"],"isi":["000486297400011"]},"date_created":"2019-09-22T22:00:36Z","publication":"Development","article_processing_charge":"No","date_published":"2019-09-12T00:00:00Z","ec_funded":1,"date_updated":"2025-05-07T11:10:55Z","title":"Root gravity response module guides differential growth determining both root bending and apical hook formation in Arabidopsis","month":"09","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"}],"article_type":"original","type":"journal_article","citation":{"apa":"Zhu, Q., Gallemi, M., Pospíšil, J., Žádníková, P., Strnad, M., &#38; Benková, E. (2019). Root gravity response module guides differential growth determining both root bending and apical hook formation in Arabidopsis. <i>Development</i>. The Company of Biologists. <a href=\"https://doi.org/10.1242/dev.175919\">https://doi.org/10.1242/dev.175919</a>","short":"Q. Zhu, M. Gallemi, J. Pospíšil, P. Žádníková, M. Strnad, E. Benková, Development 146 (2019).","ieee":"Q. Zhu, M. Gallemi, J. Pospíšil, P. Žádníková, M. Strnad, and E. Benková, “Root gravity response module guides differential growth determining both root bending and apical hook formation in Arabidopsis,” <i>Development</i>, vol. 146, no. 17. The Company of Biologists, 2019.","chicago":"Zhu, Qiang, Marçal Gallemi, Jiří Pospíšil, Petra Žádníková, Miroslav Strnad, and Eva Benková. “Root Gravity Response Module Guides Differential Growth Determining Both Root Bending and Apical Hook Formation in Arabidopsis.” <i>Development</i>. The Company of Biologists, 2019. <a href=\"https://doi.org/10.1242/dev.175919\">https://doi.org/10.1242/dev.175919</a>.","ama":"Zhu Q, Gallemi M, Pospíšil J, Žádníková P, Strnad M, Benková E. Root gravity response module guides differential growth determining both root bending and apical hook formation in Arabidopsis. <i>Development</i>. 2019;146(17). doi:<a href=\"https://doi.org/10.1242/dev.175919\">10.1242/dev.175919</a>","ista":"Zhu Q, Gallemi M, Pospíšil J, Žádníková P, Strnad M, Benková E. 2019. Root gravity response module guides differential growth determining both root bending and apical hook formation in Arabidopsis. Development. 146(17), dev175919.","mla":"Zhu, Qiang, et al. “Root Gravity Response Module Guides Differential Growth Determining Both Root Bending and Apical Hook Formation in Arabidopsis.” <i>Development</i>, vol. 146, no. 17, dev175919, The Company of Biologists, 2019, doi:<a href=\"https://doi.org/10.1242/dev.175919\">10.1242/dev.175919</a>."},"quality_controlled":"1","publication_status":"published","abstract":[{"text":"The apical hook is a transiently formed structure that plays a protective role when the germinating seedling penetrates through the soil towards the surface. Crucial for proper bending is the local auxin maxima, which defines the concave (inner) side of the hook curvature. As no sign of asymmetric auxin distribution has been reported in embryonic hypocotyls prior to hook formation, the question of how auxin asymmetry is established in the early phases of seedling germination remains largely unanswered. Here, we analyzed the auxin distribution and expression of PIN auxin efflux carriers from early phases of germination, and show that bending of the root in response to gravity is the crucial initial cue that governs the hypocotyl bending required for apical hook formation. Importantly, polar auxin transport machinery is established gradually after germination starts as a result of tight root-hypocotyl interaction and a proper balance between abscisic acid and gibberellins.","lang":"eng"}],"pmid":1,"status":"public","language":[{"iso":"eng"}],"publisher":"The Company of Biologists","day":"12","doi":"10.1242/dev.175919","oa_version":"Published Version","year":"2019","isi":1,"author":[{"id":"40A4B9E6-F248-11E8-B48F-1D18A9856A87","first_name":"Qiang","full_name":"Zhu, Qiang","last_name":"Zhu"},{"full_name":"Gallemi, Marçal","orcid":"0000-0003-4675-6893","last_name":"Gallemi","first_name":"Marçal","id":"460C6802-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Pospíšil","full_name":"Pospíšil, Jiří","first_name":"Jiří"},{"first_name":"Petra","last_name":"Žádníková","full_name":"Žádníková, Petra"},{"first_name":"Miroslav","last_name":"Strnad","full_name":"Strnad, Miroslav"},{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","first_name":"Eva","last_name":"Benková","orcid":"0000-0002-8510-9739","full_name":"Benková, Eva"}],"department":[{"_id":"EvBe"}],"publication_identifier":{"eissn":["14779129"]}},{"scopus_import":"1","oa":1,"intvolume":"         8","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","issue":"1","article_number":"10279","related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"8822"}]},"volume":8,"external_id":{"isi":["000437673200053"]},"date_created":"2018-12-11T11:45:06Z","_id":"191","project":[{"call_identifier":"FP7","grant_number":"282300","_id":"25716A02-B435-11E9-9278-68D0E5697425","name":"Polarity and subcellular dynamics in plants"},{"call_identifier":"H2020","grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425","name":"Tracing Evolution of Auxin Transport and Polarity in Plants"}],"publication":"Scientific Reports","file_date_updated":"2020-07-14T12:45:20Z","ec_funded":1,"article_processing_charge":"No","date_published":"2018-07-06T00:00:00Z","month":"07","title":"PID/WAG-mediated phosphorylation of the Arabidopsis PIN3 auxin transporter mediates polarity switches during gravitropism","date_updated":"2025-05-07T11:12:31Z","ddc":["581"],"type":"journal_article","citation":{"ieee":"P. Grones <i>et al.</i>, “PID/WAG-mediated phosphorylation of the Arabidopsis PIN3 auxin transporter mediates polarity switches during gravitropism,” <i>Scientific Reports</i>, vol. 8, no. 1. Springer, 2018.","apa":"Grones, P., Abas, M. F., Hajny, J., Jones, A., Waidmann, S., Kleine Vehn, J., &#38; Friml, J. (2018). PID/WAG-mediated phosphorylation of the Arabidopsis PIN3 auxin transporter mediates polarity switches during gravitropism. <i>Scientific Reports</i>. Springer. <a href=\"https://doi.org/10.1038/s41598-018-28188-1\">https://doi.org/10.1038/s41598-018-28188-1</a>","short":"P. Grones, M.F. Abas, J. Hajny, A. Jones, S. Waidmann, J. Kleine Vehn, J. Friml, Scientific Reports 8 (2018).","mla":"Grones, Peter, et al. “PID/WAG-Mediated Phosphorylation of the Arabidopsis PIN3 Auxin Transporter Mediates Polarity Switches during Gravitropism.” <i>Scientific Reports</i>, vol. 8, no. 1, 10279, Springer, 2018, doi:<a href=\"https://doi.org/10.1038/s41598-018-28188-1\">10.1038/s41598-018-28188-1</a>.","ista":"Grones P, Abas MF, Hajny J, Jones A, Waidmann S, Kleine Vehn J, Friml J. 2018. PID/WAG-mediated phosphorylation of the Arabidopsis PIN3 auxin transporter mediates polarity switches during gravitropism. Scientific Reports. 8(1), 10279.","ama":"Grones P, Abas MF, Hajny J, et al. PID/WAG-mediated phosphorylation of the Arabidopsis PIN3 auxin transporter mediates polarity switches during gravitropism. <i>Scientific Reports</i>. 2018;8(1). doi:<a href=\"https://doi.org/10.1038/s41598-018-28188-1\">10.1038/s41598-018-28188-1</a>","chicago":"Grones, Peter, Melinda F Abas, Jakub Hajny, Angharad Jones, Sascha Waidmann, Jürgen Kleine Vehn, and Jiří Friml. “PID/WAG-Mediated Phosphorylation of the Arabidopsis PIN3 Auxin Transporter Mediates Polarity Switches during Gravitropism.” <i>Scientific Reports</i>. Springer, 2018. <a href=\"https://doi.org/10.1038/s41598-018-28188-1\">https://doi.org/10.1038/s41598-018-28188-1</a>."},"file":[{"access_level":"open_access","file_name":"2018_ScientificReports_Grones.pdf","file_size":2413876,"content_type":"application/pdf","date_updated":"2020-07-14T12:45:20Z","relation":"main_file","checksum":"266b03f4fb8198e83141617aaa99dcab","date_created":"2018-12-17T15:38:56Z","creator":"dernst","file_id":"5714"}],"quality_controlled":"1","has_accepted_license":"1","status":"public","language":[{"iso":"eng"}],"publist_id":"7729","abstract":[{"text":"Intercellular distribution of the plant hormone auxin largely depends on the polar subcellular distribution of the plasma membrane PIN-FORMED (PIN) auxin transporters. PIN polarity switches in response to different developmental and environmental signals have been shown to redirect auxin fluxes mediating certain developmental responses. PIN phosphorylation at different sites and by different kinases is crucial for PIN function. Here we investigate the role of PIN phosphorylation during gravitropic response. Loss- and gain-of-function mutants in PINOID and related kinases but not in D6PK kinase as well as mutations mimicking constitutive dephosphorylated or phosphorylated status of two clusters of predicted phosphorylation sites partially disrupted PIN3 phosphorylation and caused defects in gravitropic bending in roots and hypocotyls. In particular, they impacted PIN3 polarity rearrangements in response to gravity and during feed-back regulation by auxin itself. Thus PIN phosphorylation, besides regulating transport activity and apical-basal targeting, is also important for the rapid polarity switches in response to environmental and endogenous signals.","lang":"eng"}],"publication_status":"published","day":"06","publisher":"Springer","oa_version":"Published Version","doi":"10.1038/s41598-018-28188-1","year":"2018","isi":1,"author":[{"full_name":"Grones, Peter","last_name":"Grones","id":"399876EC-F248-11E8-B48F-1D18A9856A87","first_name":"Peter"},{"id":"3CFB3B1C-F248-11E8-B48F-1D18A9856A87","first_name":"Melinda F","last_name":"Abas","full_name":"Abas, Melinda F"},{"id":"4800CC20-F248-11E8-B48F-1D18A9856A87","first_name":"Jakub","full_name":"Hajny, Jakub","orcid":"0000-0003-2140-7195","last_name":"Hajny"},{"last_name":"Jones","full_name":"Jones, Angharad","first_name":"Angharad"},{"full_name":"Waidmann, Sascha","last_name":"Waidmann","first_name":"Sascha"},{"full_name":"Kleine Vehn, Jürgen","last_name":"Kleine Vehn","first_name":"Jürgen"},{"full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jirí"}],"tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"department":[{"_id":"JiFr"},{"_id":"EvBe"}]},{"ddc":["570"],"file":[{"creator":"dernst","date_created":"2019-04-05T09:37:56Z","file_id":"6226","file_name":"2018_Hurny_thesis_source.docx","embargo_to":"open_access","access_level":"closed","checksum":"0c9d6d1c80d9857e6e545213467bbcb2","relation":"source_file","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","date_updated":"2020-12-02T23:30:08Z","file_size":28112114},{"file_name":"2018_Hurny_thesis.pdf","access_level":"open_access","checksum":"ecbe481a1413d270bd501b872c7ed54f","relation":"main_file","content_type":"application/pdf","date_updated":"2020-12-02T09:52:16Z","file_size":12524427,"date_created":"2019-04-05T09:37:55Z","creator":"dernst","embargo":"2019-07-10","file_id":"6227"}],"type":"dissertation","citation":{"ista":"Hurny A. 2018. Identification and characterization of novel auxin-cytokinin cross-talk components. Institute of Science and Technology Austria.","mla":"Hurny, Andrej. <i>Identification and Characterization of Novel Auxin-Cytokinin Cross-Talk Components</i>. Institute of Science and Technology Austria, 2018, doi:<a href=\"https://doi.org/10.15479/AT:ISTA:th_930\">10.15479/AT:ISTA:th_930</a>.","chicago":"Hurny, Andrej. “Identification and Characterization of Novel Auxin-Cytokinin Cross-Talk Components.” Institute of Science and Technology Austria, 2018. <a href=\"https://doi.org/10.15479/AT:ISTA:th_930\">https://doi.org/10.15479/AT:ISTA:th_930</a>.","ama":"Hurny A. Identification and characterization of novel auxin-cytokinin cross-talk components. 2018. doi:<a href=\"https://doi.org/10.15479/AT:ISTA:th_930\">10.15479/AT:ISTA:th_930</a>","ieee":"A. Hurny, “Identification and characterization of novel auxin-cytokinin cross-talk components,” Institute of Science and Technology Austria, 2018.","short":"A. Hurny, Identification and Characterization of Novel Auxin-Cytokinin Cross-Talk Components, Institute of Science and Technology Austria, 2018.","apa":"Hurny, A. (2018). <i>Identification and characterization of novel auxin-cytokinin cross-talk components</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT:ISTA:th_930\">https://doi.org/10.15479/AT:ISTA:th_930</a>"},"publist_id":"7277","abstract":[{"text":"The whole life cycle of plants as well as their responses to environmental stimuli is governed by a complex network of hormonal regulations. A number of studies have demonstrated an essential role of both auxin and cytokinin in the regulation of many aspects of plant growth and development including embryogenesis, postembryonic organogenic processes such as root, and shoot branching, root and shoot apical meristem activity and phyllotaxis. Over the last decades essential knowledge on the key molecular factors and pathways that spatio-temporally define auxin and cytokinin activities in the plant body has accumulated. However, how both hormonal pathways are interconnected by a complex network of interactions and feedback circuits that determines the final outcome of the individual hormone actions is still largely unknown. Root system architecture establishment and in particular formation of lateral organs is prime example of developmental process at whose regulation both auxin and cytokinin pathways converge. To dissect convergence points and pathways that tightly balance auxin - cytokinin antagonistic activities that determine the root branching pattern transcriptome profiling was applied. Genome wide expression analyses of the xylem pole pericycle, a tissue giving rise to lateral roots, led to identification of genes that are highly responsive to combinatorial auxin and cytokinin treatments and play an essential function in the auxin-cytokinin regulated root branching. SYNERGISTIC AUXIN CYTOKININ 1 (SYAC1) gene, which encodes for a protein of unknown function, was detected among the top candidate genes of which expression was synergistically up-regulated by simultaneous hormonal treatment. Plants with modulated SYAC1 activity exhibit severe defects in the root system establishment and attenuate developmental responses to both auxin and cytokinin. To explore the biological function of the SYAC1, we employed different strategies including expression pattern analysis, subcellular localization and phenotypic analyses of the syac1 loss-of-function and gain-of-function transgenic lines along with the identification of the SYAC1 interaction partners. Detailed functional characterization revealed that SYAC1 acts as a developmentally specific regulator of the secretory pathway to control deposition of cell wall components and thereby rapidly fine tune elongation growth.","lang":"eng"}],"publication_status":"published","status":"public","has_accepted_license":"1","language":[{"iso":"eng"}],"publisher":"Institute of Science and Technology Austria","day":"01","doi":"10.15479/AT:ISTA:th_930","oa_version":"Published Version","year":"2018","author":[{"last_name":"Hurny","orcid":"0000-0003-3638-1426","full_name":"Hurny, Andrej","id":"4DC4AF46-F248-11E8-B48F-1D18A9856A87","first_name":"Andrej"}],"publication_identifier":{"issn":["2663-337X"]},"department":[{"_id":"EvBe"}],"degree_awarded":"PhD","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"oa":1,"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","related_material":{"record":[{"id":"1024","status":"public","relation":"part_of_dissertation"}]},"alternative_title":["ISTA Thesis"],"_id":"539","date_created":"2018-12-11T11:47:03Z","supervisor":[{"last_name":"Benková","orcid":"0000-0002-8510-9739","full_name":"Benková, Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","first_name":"Eva"}],"pubrep_id":"930","file_date_updated":"2020-12-02T23:30:08Z","page":"147","article_processing_charge":"No","date_published":"2018-01-01T00:00:00Z","title":"Identification and characterization of novel auxin-cytokinin cross-talk components","date_updated":"2023-09-07T12:41:06Z","month":"01"},{"date_published":"2018-06-12T00:00:00Z","article_processing_charge":"No","title":"An armadillo-domain protein participates in a telomerase interaction network","date_updated":"2023-09-08T13:21:05Z","month":"06","_id":"277","date_created":"2018-12-11T11:45:34Z","external_id":{"isi":["000438981700009"]},"file_date_updated":"2020-07-14T12:45:45Z","publication":"Plant Molecular Biology","page":"407 - 420","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","issue":"5","oa":1,"intvolume":"        97","volume":97,"scopus_import":"1","author":[{"last_name":"Dokládal","full_name":"Dokládal, Ladislav","first_name":"Ladislav"},{"first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","last_name":"Benková","full_name":"Benková, Eva","orcid":"0000-0002-8510-9739"},{"first_name":"David","last_name":"Honys","full_name":"Honys, David"},{"full_name":"Dupláková, Nikoleta","last_name":"Dupláková","first_name":"Nikoleta"},{"last_name":"Lee","full_name":"Lee, Lan","first_name":"Lan"},{"first_name":"Stanton","full_name":"Gelvin, Stanton","last_name":"Gelvin"},{"last_name":"Sýkorová","full_name":"Sýkorová, Eva","first_name":"Eva"}],"isi":1,"year":"2018","department":[{"_id":"EvBe"}],"publisher":"Springer","day":"12","doi":"10.1007/s11103-018-0747-4","oa_version":"Submitted Version","quality_controlled":"1","abstract":[{"lang":"eng","text":"Arabidopsis and human ARM protein interact with telomerase. Deregulated mRNA levels of DNA repair and ribosomal protein genes in an Arabidopsis arm mutant suggest non-telomeric ARM function. The human homolog ARMC6 interacts with hTRF2. Abstract: Telomerase maintains telomeres and has proposed non-telomeric functions. We previously identified interaction of the C-terminal domain of Arabidopsis telomerase reverse transcriptase (AtTERT) with an armadillo/β-catenin-like repeat (ARM) containing protein. Here we explore protein–protein interactions of the ARM protein, AtTERT domains, POT1a, TRF-like family and SMH family proteins, and the chromatin remodeling protein CHR19 using bimolecular fluorescence complementation (BiFC), yeast two-hybrid (Y2H) analysis, and co-immunoprecipitation. The ARM protein interacts with both the N- and C-terminal domains of AtTERT in different cellular compartments. ARM interacts with CHR19 and TRF-like I family proteins that also bind AtTERT directly or through interaction with POT1a. The putative human ARM homolog co-precipitates telomerase activity and interacts with hTRF2 protein in vitro. Analysis of Arabidopsis arm mutants shows no obvious changes in telomere length or telomerase activity, suggesting that ARM is not essential for telomere maintenance. The observed interactions with telomerase and Myb-like domain proteins (TRF-like family I) may therefore reflect possible non-telomeric functions. Transcript levels of several DNA repair and ribosomal genes are affected in arm mutants, and ARM, likely in association with other proteins, suppressed expression of XRCC3 and RPSAA promoter constructs in luciferase reporter assays. In conclusion, ARM can participate in non-telomeric functions of telomerase, and can also perform its own telomerase-independent functions."}],"publication_status":"published","publist_id":"7625","language":[{"iso":"eng"}],"status":"public","has_accepted_license":"1","ddc":["580"],"article_type":"original","file":[{"file_size":1150679,"date_updated":"2020-07-14T12:45:45Z","content_type":"application/pdf","checksum":"451ae47616e6af2533099f596b2a47fb","relation":"main_file","access_level":"open_access","file_name":"2018_PlantMolecBio_Dokladal.pdf","file_id":"7834","date_created":"2020-05-14T12:23:08Z","creator":"dernst"}],"citation":{"ieee":"L. Dokládal <i>et al.</i>, “An armadillo-domain protein participates in a telomerase interaction network,” <i>Plant Molecular Biology</i>, vol. 97, no. 5. Springer, pp. 407–420, 2018.","apa":"Dokládal, L., Benková, E., Honys, D., Dupláková, N., Lee, L., Gelvin, S., &#38; Sýkorová, E. (2018). An armadillo-domain protein participates in a telomerase interaction network. <i>Plant Molecular Biology</i>. Springer. <a href=\"https://doi.org/10.1007/s11103-018-0747-4\">https://doi.org/10.1007/s11103-018-0747-4</a>","short":"L. Dokládal, E. Benková, D. Honys, N. Dupláková, L. Lee, S. Gelvin, E. Sýkorová, Plant Molecular Biology 97 (2018) 407–420.","mla":"Dokládal, Ladislav, et al. “An Armadillo-Domain Protein Participates in a Telomerase Interaction Network.” <i>Plant Molecular Biology</i>, vol. 97, no. 5, Springer, 2018, pp. 407–20, doi:<a href=\"https://doi.org/10.1007/s11103-018-0747-4\">10.1007/s11103-018-0747-4</a>.","ista":"Dokládal L, Benková E, Honys D, Dupláková N, Lee L, Gelvin S, Sýkorová E. 2018. An armadillo-domain protein participates in a telomerase interaction network. Plant Molecular Biology. 97(5), 407–420.","chicago":"Dokládal, Ladislav, Eva Benková, David Honys, Nikoleta Dupláková, Lan Lee, Stanton Gelvin, and Eva Sýkorová. “An Armadillo-Domain Protein Participates in a Telomerase Interaction Network.” <i>Plant Molecular Biology</i>. Springer, 2018. <a href=\"https://doi.org/10.1007/s11103-018-0747-4\">https://doi.org/10.1007/s11103-018-0747-4</a>.","ama":"Dokládal L, Benková E, Honys D, et al. An armadillo-domain protein participates in a telomerase interaction network. <i>Plant Molecular Biology</i>. 2018;97(5):407-420. doi:<a href=\"https://doi.org/10.1007/s11103-018-0747-4\">10.1007/s11103-018-0747-4</a>"},"type":"journal_article"},{"publisher":"Nature Publishing Group","day":"08","doi":"10.1038/s41598-018-27080-2","oa_version":"Published Version","year":"2018","isi":1,"author":[{"full_name":"Ceinos, Rosa Maria","last_name":"Ceinos","first_name":"Rosa Maria"},{"last_name":"Frigato","full_name":"Frigato, Elena","first_name":"Elena"},{"full_name":"Pagano, Cristina","last_name":"Pagano","first_name":"Cristina"},{"first_name":"Nadine","full_name":"Frohlich, Nadine","last_name":"Frohlich"},{"last_name":"Negrini","full_name":"Negrini, Pietro","first_name":"Pietro"},{"id":"457160E6-F248-11E8-B48F-1D18A9856A87","first_name":"Nicola","last_name":"Cavallari","full_name":"Cavallari, Nicola"},{"last_name":"Vallone","full_name":"Vallone, Daniela","first_name":"Daniela"},{"first_name":"Silvia","last_name":"Fuselli","full_name":"Fuselli, Silvia"},{"last_name":"Bertolucci","full_name":"Bertolucci, Cristiano","first_name":"Cristiano"},{"last_name":"Foulkes","full_name":"Foulkes, Nicholas S","first_name":"Nicholas S"}],"department":[{"_id":"EvBe"}],"tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"ddc":["570"],"file":[{"file_id":"5707","date_created":"2018-12-17T13:04:46Z","creator":"dernst","content_type":"application/pdf","date_updated":"2020-07-14T12:45:49Z","file_size":1855324,"checksum":"9c3942d772f84f3df032ffde0ed9a8ea","relation":"main_file","access_level":"open_access","file_name":"2018_ScientificReports_Ceinos.pdf"}],"type":"journal_article","citation":{"ieee":"R. M. Ceinos <i>et al.</i>, “Mutations in blind cavefish target the light regulated circadian clock gene period 2,” <i>Scientific Reports</i>, vol. 8, no. 1. Nature Publishing Group, 2018.","short":"R.M. Ceinos, E. Frigato, C. Pagano, N. Frohlich, P. Negrini, N. Cavallari, D. Vallone, S. Fuselli, C. Bertolucci, N.S. Foulkes, Scientific Reports 8 (2018).","apa":"Ceinos, R. M., Frigato, E., Pagano, C., Frohlich, N., Negrini, P., Cavallari, N., … Foulkes, N. S. (2018). Mutations in blind cavefish target the light regulated circadian clock gene period 2. <i>Scientific Reports</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/s41598-018-27080-2\">https://doi.org/10.1038/s41598-018-27080-2</a>","ista":"Ceinos RM, Frigato E, Pagano C, Frohlich N, Negrini P, Cavallari N, Vallone D, Fuselli S, Bertolucci C, Foulkes NS. 2018. Mutations in blind cavefish target the light regulated circadian clock gene period 2. Scientific Reports. 8(1), 8754.","mla":"Ceinos, Rosa Maria, et al. “Mutations in Blind Cavefish Target the Light Regulated Circadian Clock Gene Period 2.” <i>Scientific Reports</i>, vol. 8, no. 1, 8754, Nature Publishing Group, 2018, doi:<a href=\"https://doi.org/10.1038/s41598-018-27080-2\">10.1038/s41598-018-27080-2</a>.","ama":"Ceinos RM, Frigato E, Pagano C, et al. Mutations in blind cavefish target the light regulated circadian clock gene period 2. <i>Scientific Reports</i>. 2018;8(1). doi:<a href=\"https://doi.org/10.1038/s41598-018-27080-2\">10.1038/s41598-018-27080-2</a>","chicago":"Ceinos, Rosa Maria, Elena Frigato, Cristina Pagano, Nadine Frohlich, Pietro Negrini, Nicola Cavallari, Daniela Vallone, Silvia Fuselli, Cristiano Bertolucci, and Nicholas S Foulkes. “Mutations in Blind Cavefish Target the Light Regulated Circadian Clock Gene Period 2.” <i>Scientific Reports</i>. Nature Publishing Group, 2018. <a href=\"https://doi.org/10.1038/s41598-018-27080-2\">https://doi.org/10.1038/s41598-018-27080-2</a>."},"quality_controlled":"1","publist_id":"7616","abstract":[{"text":"Light represents the principal signal driving circadian clock entrainment. However, how light influences the evolution of the clock remains poorly understood. The cavefish Phreatichthys andruzzii represents a fascinating model to explore how evolution under extreme aphotic conditions shapes the circadian clock, since in this species the clock is unresponsive to light. We have previously demonstrated that loss-of-function mutations targeting non-visual opsins contribute in part to this blind clock phenotype. Here, we have compared orthologs of two core clock genes that play a key role in photic entrainment, cry1a and per2, in both zebrafish and P. andruzzii. We encountered aberrantly spliced variants for the P. andruzzii per2 transcript. The most abundant transcript encodes a truncated protein lacking the C-terminal Cry binding domain and incorporating an intronic, transposon-derived coding sequence. We demonstrate that the transposon insertion leads to a predominantly cytoplasmic localization of the cavefish Per2 protein in contrast to the zebrafish ortholog which is distributed in both the nucleus and cytoplasm. Thus, it seems that during evolution in complete darkness, the photic entrainment pathway of the circadian clock has been subject to mutation at multiple levels, extending from opsin photoreceptors to nuclear effectors.","lang":"eng"}],"publication_status":"published","status":"public","has_accepted_license":"1","language":[{"iso":"eng"}],"_id":"283","external_id":{"isi":["000434640800008"]},"date_created":"2018-12-11T11:45:36Z","publication":"Scientific Reports","file_date_updated":"2020-07-14T12:45:49Z","article_processing_charge":"No","date_published":"2018-06-08T00:00:00Z","title":"Mutations in blind cavefish target the light regulated circadian clock gene period 2","date_updated":"2023-09-13T08:59:27Z","month":"06","scopus_import":"1","article_number":"8754","oa":1,"intvolume":"         8","issue":"1","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","volume":8},{"volume":94,"intvolume":"        94","oa":1,"issue":"6","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","acknowledgement":"CN, DD and JHD were funded by the BBSRC (grant number BB/M009459/1). NC was funded by the VIPS Program of the Austrian Federal Ministry of Science and Research and the City of Vienna. AB and AF were supported by the Austrian Science Fund (FWF) [DK W1207; SFB RNAreg F43-P10]","scopus_import":"1","month":"06","date_updated":"2023-09-19T10:07:08Z","title":"The cyclin‐dependent kinase G group defines a thermo‐sensitive alternative splicing circuit modulating the expression of Arabidopsis ATU 2AF 65A","article_processing_charge":"No","date_published":"2018-06-01T00:00:00Z","page":"1010 - 1022","publication":"The Plant Journal","file_date_updated":"2020-07-14T12:46:22Z","external_id":{"isi":["000434365500008"]},"date_created":"2018-12-11T11:46:17Z","_id":"403","has_accepted_license":"1","status":"public","language":[{"iso":"eng"}],"publist_id":"7426","publication_status":"published","abstract":[{"lang":"eng","text":"The ability to adapt growth and development to temperature variations is crucial to generate plant varieties resilient to predicted temperature changes. However, the mechanisms underlying plant response to progressive increases in temperature have just started to be elucidated. Here, we report that the Cyclin-dependent Kinase G1 (CDKG1) is a central element in a thermo-sensitive mRNA splicing cascade that transduces changes in ambient temperature into differential expression of the fundamental spliceosome component, ATU2AF65A. CDKG1 is alternatively spliced in a temperature-dependent manner. We found that this process is partly dependent on both the Cyclin-dependent Kinase G2 (CDKG2) and the interacting co-factor CYCLIN L1 resulting in two distinct messenger RNAs. Relative abundance of both CDKG1 transcripts correlates with ambient temperature and possibly with different expression levels of the associated protein isoforms. Both CDKG1 alternative transcripts are necessary to fully complement the expression of ATU2AF65A across the temperature range. Our data support a previously unidentified temperature-dependent mechanism based on the alternative splicing of CDKG1 and regulated by CDKG2 and CYCLIN L1. We propose that changes in ambient temperature affect the relative abundance of CDKG1 transcripts and this in turn translates into differential CDKG1 protein expression coordinating the alternative splicing of ATU2AF65A. This article is protected by copyright. All rights reserved."}],"quality_controlled":"1","type":"journal_article","citation":{"short":"N. Cavallari, C. Nibau, A. Fuchs, D. Dadarou, A. Barta, J. Doonan, The Plant Journal 94 (2018) 1010–1022.","apa":"Cavallari, N., Nibau, C., Fuchs, A., Dadarou, D., Barta, A., &#38; Doonan, J. (2018). The cyclin‐dependent kinase G group defines a thermo‐sensitive alternative splicing circuit modulating the expression of Arabidopsis ATU 2AF 65A. <i>The Plant Journal</i>. Wiley. <a href=\"https://doi.org/10.1111/tpj.13914\">https://doi.org/10.1111/tpj.13914</a>","ieee":"N. Cavallari, C. Nibau, A. Fuchs, D. Dadarou, A. Barta, and J. Doonan, “The cyclin‐dependent kinase G group defines a thermo‐sensitive alternative splicing circuit modulating the expression of Arabidopsis ATU 2AF 65A,” <i>The Plant Journal</i>, vol. 94, no. 6. Wiley, pp. 1010–1022, 2018.","ama":"Cavallari N, Nibau C, Fuchs A, Dadarou D, Barta A, Doonan J. The cyclin‐dependent kinase G group defines a thermo‐sensitive alternative splicing circuit modulating the expression of Arabidopsis ATU 2AF 65A. <i>The Plant Journal</i>. 2018;94(6):1010-1022. doi:<a href=\"https://doi.org/10.1111/tpj.13914\">10.1111/tpj.13914</a>","chicago":"Cavallari, Nicola, Candida Nibau, Armin Fuchs, Despoina Dadarou, Andrea Barta, and John Doonan. “The Cyclin‐dependent Kinase G Group Defines a Thermo‐sensitive Alternative Splicing Circuit Modulating the Expression of Arabidopsis ATU 2AF 65A.” <i>The Plant Journal</i>. Wiley, 2018. <a href=\"https://doi.org/10.1111/tpj.13914\">https://doi.org/10.1111/tpj.13914</a>.","mla":"Cavallari, Nicola, et al. “The Cyclin‐dependent Kinase G Group Defines a Thermo‐sensitive Alternative Splicing Circuit Modulating the Expression of Arabidopsis ATU 2AF 65A.” <i>The Plant Journal</i>, vol. 94, no. 6, Wiley, 2018, pp. 1010–22, doi:<a href=\"https://doi.org/10.1111/tpj.13914\">10.1111/tpj.13914</a>.","ista":"Cavallari N, Nibau C, Fuchs A, Dadarou D, Barta A, Doonan J. 2018. The cyclin‐dependent kinase G group defines a thermo‐sensitive alternative splicing circuit modulating the expression of Arabidopsis ATU 2AF 65A. The Plant Journal. 94(6), 1010–1022."},"file":[{"file_id":"5934","date_created":"2019-02-06T11:40:54Z","creator":"dernst","file_size":1543354,"date_updated":"2020-07-14T12:46:22Z","content_type":"application/pdf","checksum":"d9d3ad3215ac0e581731443fca312266","relation":"main_file","access_level":"open_access","file_name":"2018_PlantJourn_Cavallari.pdf"}],"ddc":["580"],"tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"department":[{"_id":"EvBe"}],"year":"2018","author":[{"last_name":"Cavallari","full_name":"Cavallari, Nicola","first_name":"Nicola","id":"457160E6-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Candida","full_name":"Nibau, Candida","last_name":"Nibau"},{"first_name":"Armin","last_name":"Fuchs","full_name":"Fuchs, Armin"},{"full_name":"Dadarou, Despoina","last_name":"Dadarou","first_name":"Despoina"},{"full_name":"Barta, Andrea","last_name":"Barta","first_name":"Andrea"},{"last_name":"Doonan","full_name":"Doonan, John","first_name":"John"}],"isi":1,"oa_version":"Published Version","doi":"10.1111/tpj.13914","day":"01","publisher":"Wiley"},{"date_published":"2018-06-01T00:00:00Z","article_processing_charge":"No","title":"Design, synthesis and perception of fluorescently labeled isoprenoid cytokinins","date_updated":"2023-09-11T12:53:11Z","month":"06","_id":"407","date_created":"2018-12-11T11:46:18Z","external_id":{"isi":["000435623400001"]},"publication":"Phytochemistry","page":"1-11","acknowledgement":"This work was supported by the Ministry of Education Youth and Sports, Czech Republic (grant LO1204 from the National Program of Sustainability I and Agricultural Research ) and by Czech Science Foundation grants 16-04184S , 501/10/1450 and 13-39982S and by IGA projects IGA_PrF_2018_033 and IGA_PrF_2018_023 . We would like to thank Jarmila Balonová, Olga Hustáková and Miroslava Šubová for their skillful technical assistance and Mgr. Tomáš Pospíšil, Ph.D. for his measurement of 1 H NMR and analysis of some 2D NMR spectral data. \r\n","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","intvolume":"       150","volume":150,"scopus_import":"1","author":[{"last_name":"Kubiasová","full_name":"Kubiasová, Karolina","first_name":"Karolina"},{"first_name":"Václav","full_name":"Mik, Václav","last_name":"Mik"},{"last_name":"Nisler","full_name":"Nisler, Jaroslav","first_name":"Jaroslav"},{"full_name":"Hönig, Martin","last_name":"Hönig","first_name":"Martin"},{"first_name":"Alexandra","full_name":"Husičková, Alexandra","last_name":"Husičková"},{"full_name":"Spíchal, Lukáš","last_name":"Spíchal","first_name":"Lukáš"},{"first_name":"Zuzana","last_name":"Pěkná","full_name":"Pěkná, Zuzana"},{"first_name":"Olga","last_name":"Šamajová","full_name":"Šamajová, Olga"},{"first_name":"Karel","last_name":"Doležal","full_name":"Doležal, Karel"},{"first_name":"Ondřej","full_name":"Plíhal, Ondřej","last_name":"Plíhal"},{"full_name":"Benková, Eva","orcid":"0000-0002-8510-9739","last_name":"Benková","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","first_name":"Eva"},{"first_name":"Miroslav","full_name":"Strnad, Miroslav","last_name":"Strnad"},{"first_name":"Lucie","last_name":"Plíhalová","full_name":"Plíhalová, Lucie"}],"isi":1,"year":"2018","department":[{"_id":"EvBe"}],"publisher":"Elsevier","day":"01","doi":"10.1016/j.phytochem.2018.02.015","oa_version":"None","quality_controlled":"1","abstract":[{"text":"Isoprenoid cytokinins play a number of crucial roles in the regulation of plant growth and development. To study cytokinin receptor properties in plants, we designed and prepared fluorescent derivatives of 6-[(3-methylbut-2-en-1-yl)amino]purine (N6-isopentenyladenine, iP) with several fluorescent labels attached to the C2 or N9 atom of the purine moiety via a 2- or 6-carbon linker. The fluorescent labels included dansyl (DS), fluorescein (FC), 7-nitrobenzofurazan (NBD), rhodamine B (RhoB), coumarin (Cou), 7-(diethylamino)coumarin (DEAC) and cyanine 5 dye (Cy5). All prepared compounds were screened for affinity for the Arabidopsis thaliana cytokinin receptor (CRE1/AHK4). Although the attachment of the fluorescent labels to iP via the linkers mostly disrupted binding to the receptor, several fluorescent derivatives interacted well. For this reason, three derivatives, two rhodamine B and one 4-chloro-7-nitrobenzofurazan labeled iP were tested for their interaction with CRE1/AHK4 and Zea mays cytokinin receptors in detail. We further showed that the three derivatives were able to activate transcription of cytokinin response regulator ARR5 in Arabidopsis seedlings. The activity of fluorescently labeled cytokinins was compared with corresponding 6-dimethylaminopurine fluorescently labeled negative controls. Selected rhodamine B C2-labeled compounds 17, 18 and 4-chloro-7-nitrobenzofurazan N9-labeled compound 28 and their respective negative controls (19, 20 and 29, respectively) were used for in planta staining experiments in Arabidopsis thaliana cell suspension culture using live cell confocal microscopy.","lang":"eng"}],"publication_status":"published","publist_id":"7422","language":[{"iso":"eng"}],"status":"public","type":"journal_article","citation":{"apa":"Kubiasová, K., Mik, V., Nisler, J., Hönig, M., Husičková, A., Spíchal, L., … Plíhalová, L. (2018). Design, synthesis and perception of fluorescently labeled isoprenoid cytokinins. <i>Phytochemistry</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.phytochem.2018.02.015\">https://doi.org/10.1016/j.phytochem.2018.02.015</a>","short":"K. Kubiasová, V. Mik, J. Nisler, M. Hönig, A. Husičková, L. Spíchal, Z. Pěkná, O. Šamajová, K. Doležal, O. Plíhal, E. Benková, M. Strnad, L. Plíhalová, Phytochemistry 150 (2018) 1–11.","ieee":"K. Kubiasová <i>et al.</i>, “Design, synthesis and perception of fluorescently labeled isoprenoid cytokinins,” <i>Phytochemistry</i>, vol. 150. Elsevier, pp. 1–11, 2018.","chicago":"Kubiasová, Karolina, Václav Mik, Jaroslav Nisler, Martin Hönig, Alexandra Husičková, Lukáš Spíchal, Zuzana Pěkná, et al. “Design, Synthesis and Perception of Fluorescently Labeled Isoprenoid Cytokinins.” <i>Phytochemistry</i>. Elsevier, 2018. <a href=\"https://doi.org/10.1016/j.phytochem.2018.02.015\">https://doi.org/10.1016/j.phytochem.2018.02.015</a>.","ama":"Kubiasová K, Mik V, Nisler J, et al. Design, synthesis and perception of fluorescently labeled isoprenoid cytokinins. <i>Phytochemistry</i>. 2018;150:1-11. doi:<a href=\"https://doi.org/10.1016/j.phytochem.2018.02.015\">10.1016/j.phytochem.2018.02.015</a>","mla":"Kubiasová, Karolina, et al. “Design, Synthesis and Perception of Fluorescently Labeled Isoprenoid Cytokinins.” <i>Phytochemistry</i>, vol. 150, Elsevier, 2018, pp. 1–11, doi:<a href=\"https://doi.org/10.1016/j.phytochem.2018.02.015\">10.1016/j.phytochem.2018.02.015</a>.","ista":"Kubiasová K, Mik V, Nisler J, Hönig M, Husičková A, Spíchal L, Pěkná Z, Šamajová O, Doležal K, Plíhal O, Benková E, Strnad M, Plíhalová L. 2018. Design, synthesis and perception of fluorescently labeled isoprenoid cytokinins. Phytochemistry. 150, 1–11."}},{"month":"07","title":"Cup-shaped Cotyledon1 (CUC1) and CU2 regulate cytokinin homeostasis to determine ovule number in arabidopsis","date_updated":"2023-09-11T12:52:03Z","date_published":"2018-07-26T00:00:00Z","article_processing_charge":"No","page":"5169 - 5176","file_date_updated":"2020-07-14T12:46:25Z","publication":"Journal of Experimental Botany","date_created":"2018-12-11T11:44:19Z","external_id":{"isi":["000448163900015"]},"_id":"42","volume":69,"issue":"21","acknowledgement":"This work was funded by the Ministry of Education, Youth and Sports of the Czech Republic through the National Program of Sustainability (grant no. LO1204).","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","oa":1,"intvolume":"        69","scopus_import":"1","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"department":[{"_id":"EvBe"}],"isi":1,"author":[{"first_name":"Mara","last_name":"Cucinotta","full_name":"Cucinotta, Mara"},{"last_name":"Manrique","full_name":"Manrique, Silvia","first_name":"Silvia"},{"orcid":"0000-0003-1923-2410","full_name":"Cuesta, Candela","last_name":"Cuesta","first_name":"Candela","id":"33A3C818-F248-11E8-B48F-1D18A9856A87"},{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","first_name":"Eva","orcid":"0000-0002-8510-9739","full_name":"Benková, Eva","last_name":"Benková"},{"first_name":"Ondřej","last_name":"Novák","full_name":"Novák, Ondřej"},{"first_name":"Lucia","full_name":"Colombo, Lucia","last_name":"Colombo"}],"year":"2018","oa_version":"Published Version","doi":"10.1093/jxb/ery281","day":"26","publisher":"Oxford University Press","language":[{"iso":"eng"}],"has_accepted_license":"1","status":"public","abstract":[{"text":"Seeds derive from ovules upon fertilization and therefore the total number of ovules determines the final seed yield, a fundamental trait in crop plants. Among the factors that co-ordinate the process of ovule formation, the transcription factors CUP-SHAPED COTYLEDON 1 (CUC1) and CUC2 and the hormone cytokinin (CK) have a particularly prominent role. Indeed, the absence of both CUC1 and CUC2 causes a severe reduction in ovule number, a phenotype that can be rescued by CK treatment. In this study, we combined CK quantification with an integrative genome-wide target identification approach to select Arabidopsis genes regulated by CUCs that are also involved in CK metabolism. We focused our attention on the functional characterization of UDP-GLUCOSYL TRANSFERASE 85A3 (UGT85A3) and UGT73C1, which are up-regulated in the absence of CUC1 and CUC2 and encode enzymes able to catalyse CK inactivation by O-glucosylation. Our results demonstrate a role for these UGTs as a link between CUCs and CK homeostasis, and highlight the importance of CUCs and CKs in the determination of seed yield.","lang":"eng"}],"publication_status":"published","publist_id":"8012","quality_controlled":"1","citation":{"apa":"Cucinotta, M., Manrique, S., Cuesta, C., Benková, E., Novák, O., &#38; Colombo, L. (2018). Cup-shaped Cotyledon1 (CUC1) and CU2 regulate cytokinin homeostasis to determine ovule number in arabidopsis. <i>Journal of Experimental Botany</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/jxb/ery281\">https://doi.org/10.1093/jxb/ery281</a>","short":"M. Cucinotta, S. Manrique, C. Cuesta, E. Benková, O. Novák, L. Colombo, Journal of Experimental Botany 69 (2018) 5169–5176.","ieee":"M. Cucinotta, S. Manrique, C. Cuesta, E. Benková, O. Novák, and L. Colombo, “Cup-shaped Cotyledon1 (CUC1) and CU2 regulate cytokinin homeostasis to determine ovule number in arabidopsis,” <i>Journal of Experimental Botany</i>, vol. 69, no. 21. Oxford University Press, pp. 5169–5176, 2018.","ama":"Cucinotta M, Manrique S, Cuesta C, Benková E, Novák O, Colombo L. Cup-shaped Cotyledon1 (CUC1) and CU2 regulate cytokinin homeostasis to determine ovule number in arabidopsis. <i>Journal of Experimental Botany</i>. 2018;69(21):5169-5176. doi:<a href=\"https://doi.org/10.1093/jxb/ery281\">10.1093/jxb/ery281</a>","chicago":"Cucinotta, Mara, Silvia Manrique, Candela Cuesta, Eva Benková, Ondřej Novák, and Lucia Colombo. “Cup-Shaped Cotyledon1 (CUC1) and CU2 Regulate Cytokinin Homeostasis to Determine Ovule Number in Arabidopsis.” <i>Journal of Experimental Botany</i>. Oxford University Press, 2018. <a href=\"https://doi.org/10.1093/jxb/ery281\">https://doi.org/10.1093/jxb/ery281</a>.","mla":"Cucinotta, Mara, et al. “Cup-Shaped Cotyledon1 (CUC1) and CU2 Regulate Cytokinin Homeostasis to Determine Ovule Number in Arabidopsis.” <i>Journal of Experimental Botany</i>, vol. 69, no. 21, Oxford University Press, 2018, pp. 5169–76, doi:<a href=\"https://doi.org/10.1093/jxb/ery281\">10.1093/jxb/ery281</a>.","ista":"Cucinotta M, Manrique S, Cuesta C, Benková E, Novák O, Colombo L. 2018. Cup-shaped Cotyledon1 (CUC1) and CU2 regulate cytokinin homeostasis to determine ovule number in arabidopsis. Journal of Experimental Botany. 69(21), 5169–5176."},"type":"journal_article","file":[{"checksum":"ca3b6711040b1662488aeb3d1f961f13","relation":"main_file","file_size":1292128,"content_type":"application/pdf","date_updated":"2020-07-14T12:46:25Z","file_name":"2018_JournalExperimBotany_Cucinotta.pdf","access_level":"open_access","file_id":"5691","creator":"dernst","date_created":"2018-12-17T10:44:16Z"}],"ddc":["575"]},{"publist_id":"8007","publication_status":"published","abstract":[{"lang":"eng","text":"Plant hormones as signalling molecules play an essential role in the control of plant growth and development. Typically, sites of hormonal action are usually distant from the site of biosynthesis thus relying on efficient transport mechanisms. Over the last decades, molecular identification of proteins and protein complexes involved in hormonal transport has started. Advanced screens for genes involved in hormonal transport in combination with transport assays using heterologous systems such as yeast, insect, or tobacco BY2 cells or Xenopus oocytes provided important insights into mechanisms underlying distribution of hormones in plant body and led to identification of principal transporters for each hormone. This review gives a short overview of the mechanisms of hormonal transport and transporters identified in Arabidopsis thaliana."}],"status":"public","language":[{"iso":"eng"}],"quality_controlled":"1","citation":{"ista":"Abualia R, Benková E, Lacombe B. 2018. Transporters and mechanisms of hormone transport in arabidopsis. Advances in Botanical Research. 87, 115–138.","mla":"Abualia, Rashed, et al. “Transporters and Mechanisms of Hormone Transport in Arabidopsis.” <i>Advances in Botanical Research</i>, vol. 87, Elsevier, 2018, pp. 115–38, doi:<a href=\"https://doi.org/10.1016/bs.abr.2018.09.007\">10.1016/bs.abr.2018.09.007</a>.","ama":"Abualia R, Benková E, Lacombe B. Transporters and mechanisms of hormone transport in arabidopsis. <i>Advances in Botanical Research</i>. 2018;87:115-138. doi:<a href=\"https://doi.org/10.1016/bs.abr.2018.09.007\">10.1016/bs.abr.2018.09.007</a>","chicago":"Abualia, Rashed, Eva Benková, and Benoît Lacombe. “Transporters and Mechanisms of Hormone Transport in Arabidopsis.” <i>Advances in Botanical Research</i>. Elsevier, 2018. <a href=\"https://doi.org/10.1016/bs.abr.2018.09.007\">https://doi.org/10.1016/bs.abr.2018.09.007</a>.","ieee":"R. Abualia, E. Benková, and B. Lacombe, “Transporters and mechanisms of hormone transport in arabidopsis,” <i>Advances in Botanical Research</i>, vol. 87. Elsevier, pp. 115–138, 2018.","apa":"Abualia, R., Benková, E., &#38; Lacombe, B. (2018). Transporters and mechanisms of hormone transport in arabidopsis. <i>Advances in Botanical Research</i>. Elsevier. <a href=\"https://doi.org/10.1016/bs.abr.2018.09.007\">https://doi.org/10.1016/bs.abr.2018.09.007</a>","short":"R. Abualia, E. Benková, B. Lacombe, Advances in Botanical Research 87 (2018) 115–138."},"type":"journal_article","department":[{"_id":"EvBe"}],"year":"2018","author":[{"first_name":"Rashed","id":"4827E134-F248-11E8-B48F-1D18A9856A87","last_name":"Abualia","full_name":"Abualia, Rashed","orcid":"0000-0002-9357-9415"},{"last_name":"Benková","orcid":"0000-0002-8510-9739","full_name":"Benková, Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","first_name":"Eva"},{"first_name":"Benoît","full_name":"Lacombe, Benoît","last_name":"Lacombe"}],"isi":1,"doi":"10.1016/bs.abr.2018.09.007","oa_version":"None","publisher":"Elsevier","day":"01","related_material":{"record":[{"relation":"dissertation_contains","status":"public","id":"10303"}]},"volume":87,"intvolume":"        87","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","scopus_import":"1","date_updated":"2024-03-25T23:30:22Z","title":"Transporters and mechanisms of hormone transport in arabidopsis","month":"01","article_processing_charge":"No","date_published":"2018-01-01T00:00:00Z","publication":"Advances in Botanical Research","page":"115 - 138","_id":"47","external_id":{"isi":["000453657800006"]},"date_created":"2018-12-11T11:44:20Z"},{"scopus_import":"1","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","oa":1,"intvolume":"        45","volume":45,"_id":"1004","project":[{"name":"Hormone cross-talk drives nutrient dependent plant development","grant_number":"I 1774-B16","_id":"2542D156-B435-11E9-9278-68D0E5697425","call_identifier":"FWF"}],"date_created":"2018-12-11T11:49:38Z","external_id":{"pmid":["28391060"],"isi":["000404880400013"]},"pubrep_id":"1017","file_date_updated":"2019-04-17T08:00:36Z","publication":"Current Opinion in Genetics & Development","page":"82 - 89","date_published":"2017-08-01T00:00:00Z","article_processing_charge":"No","date_updated":"2023-09-22T09:48:15Z","title":"Spatiotemporal mechanisms of root branching","month":"08","ddc":["575"],"file":[{"date_created":"2019-04-17T08:00:36Z","creator":"dernst","file_id":"6336","file_name":"Otvos_Benkova_CurOpDevBiol_2017.pdf","access_level":"open_access","success":1,"relation":"main_file","file_size":364133,"content_type":"application/pdf","date_updated":"2019-04-17T08:00:36Z"}],"type":"journal_article","citation":{"mla":"Ötvös, Krisztina, and Eva Benková. “Spatiotemporal Mechanisms of Root Branching.” <i>Current Opinion in Genetics &#38; Development</i>, vol. 45, Elsevier, 2017, pp. 82–89, doi:<a href=\"https://doi.org/10.1016/j.gde.2017.03.010\">10.1016/j.gde.2017.03.010</a>.","ista":"Ötvös K, Benková E. 2017. Spatiotemporal mechanisms of root branching. Current Opinion in Genetics &#38; Development. 45, 82–89.","ama":"Ötvös K, Benková E. Spatiotemporal mechanisms of root branching. <i>Current Opinion in Genetics &#38; Development</i>. 2017;45:82-89. doi:<a href=\"https://doi.org/10.1016/j.gde.2017.03.010\">10.1016/j.gde.2017.03.010</a>","chicago":"Ötvös, Krisztina, and Eva Benková. “Spatiotemporal Mechanisms of Root Branching.” <i>Current Opinion in Genetics &#38; Development</i>. Elsevier, 2017. <a href=\"https://doi.org/10.1016/j.gde.2017.03.010\">https://doi.org/10.1016/j.gde.2017.03.010</a>.","ieee":"K. Ötvös and E. Benková, “Spatiotemporal mechanisms of root branching,” <i>Current Opinion in Genetics &#38; Development</i>, vol. 45. Elsevier, pp. 82–89, 2017.","short":"K. Ötvös, E. Benková, Current Opinion in Genetics &#38; Development 45 (2017) 82–89.","apa":"Ötvös, K., &#38; Benková, E. (2017). Spatiotemporal mechanisms of root branching. <i>Current Opinion in Genetics &#38; Development</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.gde.2017.03.010\">https://doi.org/10.1016/j.gde.2017.03.010</a>"},"quality_controlled":"1","publication_status":"published","abstract":[{"lang":"eng","text":"The fundamental tasks of the root system are, besides anchoring, mediating interactions between plant and soil and providing the plant with water and nutrients. The architecture of the root system is controlled by endogenous mechanisms that constantly integrate environmental signals, such as availability of nutrients and water. Extremely important for efficient soil exploitation and survival under less favorable conditions is the developmental flexibility of the root system that is largely determined by its postembryonic branching capacity. Modulation of initiation and outgrowth of lateral roots provides roots with an exceptional plasticity, allows optimal adjustment to underground heterogeneity, and enables effective soil exploitation and use of resources. Here we discuss recent advances in understanding the molecular mechanisms that shape the plant root system and integrate external cues to adapt to the changing environment."}],"publist_id":"6394","language":[{"iso":"eng"}],"pmid":1,"status":"public","has_accepted_license":"1","publisher":"Elsevier","day":"01","doi":"10.1016/j.gde.2017.03.010","oa_version":"Submitted Version","author":[{"id":"29B901B0-F248-11E8-B48F-1D18A9856A87","first_name":"Krisztina","last_name":"Ötvös","orcid":"0000-0002-5503-4983","full_name":"Ötvös, Krisztina"},{"full_name":"Benková, Eva","orcid":"0000-0002-8510-9739","last_name":"Benková","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","first_name":"Eva"}],"isi":1,"year":"2017","publication_identifier":{"issn":["0959437X"]},"department":[{"_id":"EvBe"}],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png"}},{"department":[{"_id":"EvBe"}],"year":"2017","author":[{"first_name":"Tereza","full_name":"Dobisova, Tereza","last_name":"Dobisova"},{"first_name":"Vendula","full_name":"Hrdinova, Vendula","last_name":"Hrdinova"},{"full_name":"Cuesta, Candela","orcid":"0000-0003-1923-2410","last_name":"Cuesta","first_name":"Candela","id":"33A3C818-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Michlickova","full_name":"Michlickova, Sarka","first_name":"Sarka"},{"last_name":"Urbankova","full_name":"Urbankova, Ivana","first_name":"Ivana"},{"full_name":"Hejatkova, Romana","last_name":"Hejatkova","first_name":"Romana"},{"first_name":"Petra","last_name":"Zadnikova","full_name":"Zadnikova, Petra"},{"first_name":"Markéta","full_name":"Pernisová, Markéta","last_name":"Pernisová"},{"first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8510-9739","full_name":"Benková, Eva","last_name":"Benková"},{"last_name":"Hejátko","full_name":"Hejátko, Jan","first_name":"Jan"}],"isi":1,"oa_version":"None","doi":"10.1104/pp.16.01964","day":"17","publisher":"American Society of Plant Biologists","status":"public","language":[{"iso":"eng"}],"publist_id":"6375","publication_status":"published","abstract":[{"lang":"eng","text":"In plants, the multistep phosphorelay (MSP) pathway mediates a range of regulatory processes, including those activated by cytokinins. The crosstalk between cytokinin response and light is known for a long time. However, the molecular mechanism underlying the interactionbetween light and cytokinin signaling remains elusive. In the screen for upstream regulators we identified a LONG PALE HYPOCOTYL (LPH) gene whose activity is indispensable for spatiotemporally correct expression of CYTOKININ INDEPENDENT-1 (CKI1), encoding the constitutively active sensor histidine kinase that activates MSP signaling. lph is a new allele of HEME OXYGENASE 1 (HY1) which encodes the key protein in the biosynthesis of phytochromobilin, a cofactor of photoconvertiblephytochromes. Our analysis confirmed the light-dependent regulation oftheCKI1 expression pattern. We show that CKI1 expression is under the control of phytochrome A (phyA), functioning as a dual (both positive and negative) regulator of CKI1 expression, presumably via the phyA-regulated transcription factors PHYTOCHROME INTERACTING FACTOR 3 (PIF3) and CIRCADIAN CLOCK ASSOCIATED 1 (CCA1). Changes in CKI1 expression observed in lph/hy1-7 and phy mutants correlatewithmisregulation of MSP signaling, changedcytokinin sensitivity and developmental aberrations,previously shown to be associated with cytokinin and/or CKI1 action. Besides that, we demonstrate novel role of phyA-dependent CKI1 expression in the hypocotyl elongation and hook development during skotomorphogenesis. Based on these results, we propose that the light-dependent regulation of CKI1 provides a plausible mechanistic link underlying the well-known interaction between light- and cytokinin-controlled plant development."}],"quality_controlled":"1","type":"journal_article","citation":{"mla":"Dobisova, Tereza, et al. “Light Regulated Expression of Sensor Histidine Kinase CKI1 Controls Cytokinin Related Development.” <i>Plant Physiology</i>, vol. 174, no. 1, American Society of Plant Biologists, 2017, pp. 387–404, doi:<a href=\"https://doi.org/10.1104/pp.16.01964\">10.1104/pp.16.01964</a>.","ista":"Dobisova T, Hrdinova V, Cuesta C, Michlickova S, Urbankova I, Hejatkova R, Zadnikova P, Pernisová M, Benková E, Hejátko J. 2017. Light regulated expression of sensor histidine kinase CKI1 controls cytokinin related development. Plant Physiology. 174(1), 387–404.","chicago":"Dobisova, Tereza, Vendula Hrdinova, Candela Cuesta, Sarka Michlickova, Ivana Urbankova, Romana Hejatkova, Petra Zadnikova, Markéta Pernisová, Eva Benková, and Jan Hejátko. “Light Regulated Expression of Sensor Histidine Kinase CKI1 Controls Cytokinin Related Development.” <i>Plant Physiology</i>. American Society of Plant Biologists, 2017. <a href=\"https://doi.org/10.1104/pp.16.01964\">https://doi.org/10.1104/pp.16.01964</a>.","ama":"Dobisova T, Hrdinova V, Cuesta C, et al. Light regulated expression of sensor histidine kinase CKI1 controls cytokinin related development. <i>Plant Physiology</i>. 2017;174(1):387-404. doi:<a href=\"https://doi.org/10.1104/pp.16.01964\">10.1104/pp.16.01964</a>","ieee":"T. Dobisova <i>et al.</i>, “Light regulated expression of sensor histidine kinase CKI1 controls cytokinin related development,” <i>Plant Physiology</i>, vol. 174, no. 1. American Society of Plant Biologists, pp. 387–404, 2017.","apa":"Dobisova, T., Hrdinova, V., Cuesta, C., Michlickova, S., Urbankova, I., Hejatkova, R., … Hejátko, J. (2017). Light regulated expression of sensor histidine kinase CKI1 controls cytokinin related development. <i>Plant Physiology</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1104/pp.16.01964\">https://doi.org/10.1104/pp.16.01964</a>","short":"T. Dobisova, V. Hrdinova, C. Cuesta, S. Michlickova, I. Urbankova, R. Hejatkova, P. Zadnikova, M. Pernisová, E. Benková, J. Hejátko, Plant Physiology 174 (2017) 387–404."},"month":"05","title":"Light regulated expression of sensor histidine kinase CKI1 controls cytokinin related development","date_updated":"2023-09-22T09:41:48Z","article_processing_charge":"No","date_published":"2017-05-17T00:00:00Z","page":"387 - 404","publication":"Plant Physiology","external_id":{"isi":["000402057200028"]},"date_created":"2018-12-11T11:49:43Z","_id":"1018","volume":174,"intvolume":"       174","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","issue":"1","scopus_import":"1"}]