[{"ddc":["580"],"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"isi":1,"article_number":"586870","title":"A functional kinase is necessary for cyclin-dependent kinase G1 (CDKG1) to maintain fertility at high ambient temperature in Arabidopsis","external_id":{"isi":["000591637000001"]},"year":"2020","doi":"10.3389/fpls.2020.586870","publication_identifier":{"eissn":["1664-462X"]},"_id":"8924","quality_controlled":"1","oa_version":"Published Version","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","acknowledgement":"CN, DD, NF-F, and JD were funded by the BBSRC (grant number BB/M009459/1). NK and AM were funded through the ERASMUS+Program. NC was funded by the VIPS Program of the Austrian Federal Ministry of Science and Research and the City of Vienna.","article_processing_charge":"No","volume":11,"date_updated":"2023-08-24T10:50:00Z","oa":1,"abstract":[{"text":"Maintaining fertility in a fluctuating environment is key to the reproductive success of flowering plants. Meiosis and pollen formation are particularly sensitive to changes in growing conditions, especially temperature. We have previously identified cyclin-dependent kinase G1 (CDKG1) as a master regulator of temperature-dependent meiosis and this may involve the regulation of alternative splicing (AS), including of its own transcript. CDKG1 mRNA can undergo several AS events, potentially producing two protein variants: CDKG1L and CDKG1S, differing in their N-terminal domain which may be involved in co-factor interaction. In leaves, both isoforms have distinct temperature-dependent functions on target mRNA processing, but their role in pollen development is unknown. In the present study, we characterize the role of CDKG1L and CDKG1S in maintaining Arabidopsis fertility. We show that the long (L) form is necessary and sufficient to rescue the fertility defects of the cdkg1-1 mutant, while the short (S) form is unable to rescue fertility. On the other hand, an extra copy of CDKG1L reduces fertility. In addition, mutation of the ATP binding pocket of the kinase indicates that kinase activity is necessary for the function of CDKG1. Kinase mutants of CDKG1L and CDKG1S correctly localize to the cell nucleus and nucleus and cytoplasm, respectively, but are unable to rescue either the fertility or the splicing defects of the cdkg1-1 mutant. Furthermore, we show that there is partial functional overlap between CDKG1 and its paralog CDKG2 that could in part be explained by overlapping gene expression.","lang":"eng"}],"author":[{"first_name":"Candida","last_name":"Nibau","full_name":"Nibau, Candida"},{"full_name":"Dadarou, Despoina","last_name":"Dadarou","first_name":"Despoina"},{"last_name":"Kargios","full_name":"Kargios, Nestoras","first_name":"Nestoras"},{"full_name":"Mallioura, Areti","last_name":"Mallioura","first_name":"Areti"},{"first_name":"Narcis","last_name":"Fernandez-Fuentes","full_name":"Fernandez-Fuentes, Narcis"},{"first_name":"Nicola","full_name":"Cavallari, Nicola","last_name":"Cavallari","id":"457160E6-F248-11E8-B48F-1D18A9856A87"},{"first_name":"John H.","full_name":"Doonan, John H.","last_name":"Doonan"}],"citation":{"chicago":"Nibau, Candida, Despoina Dadarou, Nestoras Kargios, Areti Mallioura, Narcis Fernandez-Fuentes, Nicola Cavallari, and John H. Doonan. “A Functional Kinase Is Necessary for Cyclin-Dependent Kinase G1 (CDKG1) to Maintain Fertility at High Ambient Temperature in Arabidopsis.” <i>Frontiers in Plant Science</i>. Frontiers, 2020. <a href=\"https://doi.org/10.3389/fpls.2020.586870\">https://doi.org/10.3389/fpls.2020.586870</a>.","ieee":"C. Nibau <i>et al.</i>, “A functional kinase is necessary for cyclin-dependent kinase G1 (CDKG1) to maintain fertility at high ambient temperature in Arabidopsis,” <i>Frontiers in Plant Science</i>, vol. 11. Frontiers, 2020.","apa":"Nibau, C., Dadarou, D., Kargios, N., Mallioura, A., Fernandez-Fuentes, N., Cavallari, N., &#38; Doonan, J. H. (2020). A functional kinase is necessary for cyclin-dependent kinase G1 (CDKG1) to maintain fertility at high ambient temperature in Arabidopsis. <i>Frontiers in Plant Science</i>. Frontiers. <a href=\"https://doi.org/10.3389/fpls.2020.586870\">https://doi.org/10.3389/fpls.2020.586870</a>","short":"C. Nibau, D. Dadarou, N. Kargios, A. Mallioura, N. Fernandez-Fuentes, N. Cavallari, J.H. Doonan, Frontiers in Plant Science 11 (2020).","ista":"Nibau C, Dadarou D, Kargios N, Mallioura A, Fernandez-Fuentes N, Cavallari N, Doonan JH. 2020. A functional kinase is necessary for cyclin-dependent kinase G1 (CDKG1) to maintain fertility at high ambient temperature in Arabidopsis. Frontiers in Plant Science. 11, 586870.","ama":"Nibau C, Dadarou D, Kargios N, et al. A functional kinase is necessary for cyclin-dependent kinase G1 (CDKG1) to maintain fertility at high ambient temperature in Arabidopsis. <i>Frontiers in Plant Science</i>. 2020;11. doi:<a href=\"https://doi.org/10.3389/fpls.2020.586870\">10.3389/fpls.2020.586870</a>","mla":"Nibau, Candida, et al. “A Functional Kinase Is Necessary for Cyclin-Dependent Kinase G1 (CDKG1) to Maintain Fertility at High Ambient Temperature in Arabidopsis.” <i>Frontiers in Plant Science</i>, vol. 11, 586870, Frontiers, 2020, doi:<a href=\"https://doi.org/10.3389/fpls.2020.586870\">10.3389/fpls.2020.586870</a>."},"publication_status":"published","date_created":"2020-12-06T23:01:14Z","file":[{"file_id":"8929","creator":"dernst","content_type":"application/pdf","relation":"main_file","success":1,"date_updated":"2020-12-09T09:14:19Z","access_level":"open_access","date_created":"2020-12-09T09:14:19Z","checksum":"1c0ee6ce9950aa665d6a5cc64aa6b752","file_name":"2020_Frontiers_Nibau.pdf","file_size":1833244}],"has_accepted_license":"1","department":[{"_id":"EvBe"}],"scopus_import":"1","publisher":"Frontiers","language":[{"iso":"eng"}],"month":"11","date_published":"2020-11-10T00:00:00Z","article_type":"original","publication":"Frontiers in Plant Science","file_date_updated":"2020-12-09T09:14:19Z","intvolume":"        11","status":"public","day":"10","type":"journal_article"},{"month":"01","article_type":"original","date_published":"2020-01-22T00:00:00Z","scopus_import":"1","publisher":"Frontiers Media","language":[{"iso":"eng"}],"has_accepted_license":"1","department":[{"_id":"EvBe"}],"date_created":"2020-01-22T15:23:57Z","file":[{"access_level":"open_access","date_updated":"2020-07-14T12:47:56Z","file_size":1951438,"file_name":"2020_FrontiersPlantScience_Nibau.pdf","checksum":"d1f92e60a713fbd15097ce895e5c7ccb","date_created":"2020-01-27T09:07:02Z","relation":"main_file","content_type":"application/pdf","file_id":"7366","creator":"dernst"}],"day":"22","type":"journal_article","intvolume":"        10","status":"public","publication":"Frontiers in Plant Science","file_date_updated":"2020-07-14T12:47:56Z","year":"2020","doi":"10.3389/fpls.2019.01680","external_id":{"isi":["000511376000001"]},"title":"Thermo-sensitive alternative splicing of FLOWERING LOCUS M is modulated by cyclin-dependent kinase G2","tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"isi":1,"article_number":"1680","ddc":["580"],"citation":{"ieee":"C. Nibau, M. Gallemi, D. Dadarou, J. H. Doonan, and N. Cavallari, “Thermo-sensitive alternative splicing of FLOWERING LOCUS M is modulated by cyclin-dependent kinase G2,” <i>Frontiers in Plant Science</i>, vol. 10. Frontiers Media, 2020.","apa":"Nibau, C., Gallemi, M., Dadarou, D., Doonan, J. H., &#38; Cavallari, N. (2020). Thermo-sensitive alternative splicing of FLOWERING LOCUS M is modulated by cyclin-dependent kinase G2. <i>Frontiers in Plant Science</i>. Frontiers Media. <a href=\"https://doi.org/10.3389/fpls.2019.01680\">https://doi.org/10.3389/fpls.2019.01680</a>","chicago":"Nibau, Candida, Marçal Gallemi, Despoina Dadarou, John H. Doonan, and Nicola Cavallari. “Thermo-Sensitive Alternative Splicing of FLOWERING LOCUS M Is Modulated by Cyclin-Dependent Kinase G2.” <i>Frontiers in Plant Science</i>. Frontiers Media, 2020. <a href=\"https://doi.org/10.3389/fpls.2019.01680\">https://doi.org/10.3389/fpls.2019.01680</a>.","mla":"Nibau, Candida, et al. “Thermo-Sensitive Alternative Splicing of FLOWERING LOCUS M Is Modulated by Cyclin-Dependent Kinase G2.” <i>Frontiers in Plant Science</i>, vol. 10, 1680, Frontiers Media, 2020, doi:<a href=\"https://doi.org/10.3389/fpls.2019.01680\">10.3389/fpls.2019.01680</a>.","ama":"Nibau C, Gallemi M, Dadarou D, Doonan JH, Cavallari N. Thermo-sensitive alternative splicing of FLOWERING LOCUS M is modulated by cyclin-dependent kinase G2. <i>Frontiers in Plant Science</i>. 2020;10. doi:<a href=\"https://doi.org/10.3389/fpls.2019.01680\">10.3389/fpls.2019.01680</a>","short":"C. Nibau, M. Gallemi, D. Dadarou, J.H. Doonan, N. Cavallari, Frontiers in Plant Science 10 (2020).","ista":"Nibau C, Gallemi M, Dadarou D, Doonan JH, Cavallari N. 2020. Thermo-sensitive alternative splicing of FLOWERING LOCUS M is modulated by cyclin-dependent kinase G2. Frontiers in Plant Science. 10, 1680."},"publication_status":"published","abstract":[{"lang":"eng","text":"The ability to sense environmental temperature and to coordinate growth and development accordingly, is critical to the reproductive success of plants. Flowering time is regulated at the level of gene expression by a complex network of factors that integrate environmental and developmental cues. One of the main players, involved in modulating flowering time in response to changes in ambient temperature is FLOWERING LOCUS M (FLM). FLM transcripts can undergo extensive alternative splicing producing multiple variants, of which FLM-β and FLM-δ are the most representative. While FLM-β codes for the flowering repressor FLM protein, translation of FLM-δ has the opposite effect on flowering. Here we show that the cyclin-dependent kinase G2 (CDKG2), together with its cognate cyclin, CYCLYN L1 (CYCL1) affects the alternative splicing of FLM, balancing the levels of FLM-β and FLM-δ across the ambient temperature range. In the absence of the CDKG2/CYCL1 complex, FLM-β expression is reduced while FLM-δ is increased in a temperature dependent manner and these changes are associated with an early flowering phenotype in the cdkg2 mutant lines. In addition, we found that transcript variants retaining the full FLM intron 1 are sequestered in the cell nucleus. Strikingly, FLM intron 1 splicing is also regulated by CDKG2/CYCL1. Our results provide evidence that temperature and CDKs regulate the alternative splicing of FLM, contributing to flowering time definition."}],"author":[{"first_name":"Candida","last_name":"Nibau","full_name":"Nibau, Candida"},{"orcid":"0000-0003-4675-6893","full_name":"Gallemi, Marçal","last_name":"Gallemi","first_name":"Marçal","id":"460C6802-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Dadarou","full_name":"Dadarou, Despoina","first_name":"Despoina"},{"full_name":"Doonan, John H.","last_name":"Doonan","first_name":"John H."},{"first_name":"Nicola","full_name":"Cavallari, Nicola","last_name":"Cavallari","id":"457160E6-F248-11E8-B48F-1D18A9856A87"}],"article_processing_charge":"No","date_updated":"2023-08-17T14:21:45Z","oa":1,"volume":10,"publication_identifier":{"issn":["1664-462X"]},"_id":"7350","oa_version":"Published Version","quality_controlled":"1","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8"},{"citation":{"short":"S. Tan, M.F. Abas, I. Verstraeten, M. Glanc, G. Molnar, J. Hajny, P. Lasák, I. Petřík, E. Russinova, J. Petrášek, O. Novák, J. Pospíšil, J. Friml, Current Biology 30 (2020) 381–395.e8.","ista":"Tan S, Abas MF, Verstraeten I, Glanc M, Molnar G, Hajny J, Lasák P, Petřík I, Russinova E, Petrášek J, Novák O, Pospíšil J, Friml J. 2020. Salicylic acid targets protein phosphatase 2A to attenuate growth in plants. Current Biology. 30(3), 381–395.e8.","mla":"Tan, Shutang, et al. “Salicylic Acid Targets Protein Phosphatase 2A to Attenuate Growth in Plants.” <i>Current Biology</i>, vol. 30, no. 3, Cell Press, 2020, p. 381–395.e8, doi:<a href=\"https://doi.org/10.1016/j.cub.2019.11.058\">10.1016/j.cub.2019.11.058</a>.","ama":"Tan S, Abas MF, Verstraeten I, et al. Salicylic acid targets protein phosphatase 2A to attenuate growth in plants. <i>Current Biology</i>. 2020;30(3):381-395.e8. doi:<a href=\"https://doi.org/10.1016/j.cub.2019.11.058\">10.1016/j.cub.2019.11.058</a>","chicago":"Tan, Shutang, Melinda F Abas, Inge Verstraeten, Matous Glanc, Gergely Molnar, Jakub Hajny, Pavel Lasák, et al. “Salicylic Acid Targets Protein Phosphatase 2A to Attenuate Growth in Plants.” <i>Current Biology</i>. Cell Press, 2020. <a href=\"https://doi.org/10.1016/j.cub.2019.11.058\">https://doi.org/10.1016/j.cub.2019.11.058</a>.","ieee":"S. Tan <i>et al.</i>, “Salicylic acid targets protein phosphatase 2A to attenuate growth in plants,” <i>Current Biology</i>, vol. 30, no. 3. Cell Press, p. 381–395.e8, 2020.","apa":"Tan, S., Abas, M. F., Verstraeten, I., Glanc, M., Molnar, G., Hajny, J., … Friml, J. (2020). Salicylic acid targets protein phosphatase 2A to attenuate growth in plants. <i>Current Biology</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cub.2019.11.058\">https://doi.org/10.1016/j.cub.2019.11.058</a>"},"publication_status":"published","author":[{"id":"2DE75584-F248-11E8-B48F-1D18A9856A87","first_name":"Shutang","full_name":"Tan, Shutang","last_name":"Tan","orcid":"0000-0002-0471-8285"},{"id":"3CFB3B1C-F248-11E8-B48F-1D18A9856A87","full_name":"Abas, Melinda F","last_name":"Abas","first_name":"Melinda F"},{"id":"362BF7FE-F248-11E8-B48F-1D18A9856A87","first_name":"Inge","orcid":"0000-0001-7241-2328","full_name":"Verstraeten, Inge","last_name":"Verstraeten"},{"id":"1AE1EA24-02D0-11E9-9BAA-DAF4881429F2","first_name":"Matous","orcid":"0000-0003-0619-7783","last_name":"Glanc","full_name":"Glanc, Matous"},{"id":"34F1AF46-F248-11E8-B48F-1D18A9856A87","first_name":"Gergely","last_name":"Molnar","full_name":"Molnar, Gergely"},{"id":"4800CC20-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2140-7195","full_name":"Hajny, Jakub","last_name":"Hajny","first_name":"Jakub"},{"last_name":"Lasák","full_name":"Lasák, Pavel","first_name":"Pavel"},{"first_name":"Ivan","full_name":"Petřík, Ivan","last_name":"Petřík"},{"full_name":"Russinova, Eugenia","last_name":"Russinova","first_name":"Eugenia"},{"first_name":"Jan","full_name":"Petrášek, Jan","last_name":"Petrášek"},{"full_name":"Novák, Ondřej","last_name":"Novák","first_name":"Ondřej"},{"first_name":"Jiří","full_name":"Pospíšil, Jiří","last_name":"Pospíšil"},{"first_name":"Jiří","last_name":"Friml","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"abstract":[{"text":"Plants, like other multicellular organisms, survive through a delicate balance between growth and defense against pathogens. Salicylic acid (SA) is a major defense signal in plants, and the perception mechanism as well as downstream signaling activating the immune response are known. Here, we identify a parallel SA signaling that mediates growth attenuation. SA directly binds to A subunits of protein phosphatase 2A (PP2A), inhibiting activity of this complex. Among PP2A targets, the PIN2 auxin transporter is hyperphosphorylated in response to SA, leading to changed activity of this important growth regulator. Accordingly, auxin transport and auxin-mediated root development, including growth, gravitropic response, and lateral root organogenesis, are inhibited. This study reveals how SA, besides activating immunity, concomitantly attenuates growth through crosstalk with the auxin distribution network. Further analysis of this dual role of SA and characterization of additional SA-regulated PP2A targets will provide further insights into mechanisms maintaining a balance between growth and defense.","lang":"eng"}],"article_processing_charge":"No","oa":1,"date_updated":"2024-03-25T23:30:20Z","volume":30,"oa_version":"Published Version","quality_controlled":"1","project":[{"call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","_id":"261099A6-B435-11E9-9278-68D0E5697425","grant_number":"742985"},{"grant_number":"291734","name":"International IST Postdoc Fellowship Programme","_id":"25681D80-B435-11E9-9278-68D0E5697425","call_identifier":"FP7"},{"grant_number":"723-2015","_id":"256FEF10-B435-11E9-9278-68D0E5697425","name":"Long Term Fellowship"}],"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","acknowledgement":"We thank Shigeyuki Betsuyaku (University of Tsukuba), Alison Delong (Brown University), Xinnian Dong (Duke University), Dolf Weijers (Wageningen University), Yuelin Zhang (UBC), and Martine Pastuglia (Institut Jean-Pierre Bourgin) for sharing published materials; Jana Riederer for help with cantharidin physiological analysis; David Domjan for help with cloning pET28a-PIN2HL; Qing Lu for help with DARTS; Hana Kozubı´kova´ for technical support on SA derivative synthesis; Zuzana Vondra´ kova´ for technical support with tobacco cells; Lucia Strader (Washington University), Bert De Rybel (Ghent University), Bartel Vanholme (Ghent University), and Lukas Mach (BOKU) for helpful discussions; and bioimaging and life science facilities of IST Austria for continuous support. We gratefully acknowledge the Nottingham Arabidopsis Stock Center (NASC) for providing T-DNA insertional mutants. The DSC and SPR instruments were provided by the EQ-BOKU VIBT GmbH and the BOKU Core Facility for Biomolecular and Cellular Analysis, with help of Irene Schaffner. The research leading to these results has received funding from the European Union’s Horizon 2020 program (ERC grant agreement no. 742985 to J.F.) and the People Programme (Marie Curie Actions) of the European Union’s Seventh Framework Programme (FP7/2007-2013) under REA grant agreement no. 291734. S.T. was supported by a European Molecular Biology Organization (EMBO) long-term postdoctoral fellowship (ALTF 723-2015). O.N. was supported by the Ministry of Education, Youth and Sports of the Czech Republic (European Regional Development Fund-Project ‘‘Centre for Experimental Plant Biology’’ no. CZ.02.1.01/0.0/0.0/16_019/0000738). J. Pospısil was supported by European Regional Development Fund Project ‘‘Centre for Experimental Plant Biology’’\r\n(no. CZ.02.1.01/0.0/0.0/16_019/0000738). J. Petrasek was supported by EU Operational Programme Prague-Competitiveness (no. CZ.2.16/3.1.00/21519). ","publication_identifier":{"issn":["09609822"]},"_id":"7427","pmid":1,"ec_funded":1,"doi":"10.1016/j.cub.2019.11.058","year":"2020","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"title":"Salicylic acid targets protein phosphatase 2A to attenuate growth in plants","external_id":{"isi":["000511287900018"],"pmid":["31956021"]},"isi":1,"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"ddc":["580"],"related_material":{"record":[{"id":"8822","relation":"dissertation_contains","status":"public"}]},"type":"journal_article","day":"03","status":"public","intvolume":"        30","file_date_updated":"2020-09-22T09:51:28Z","page":"381-395.e8","publication":"Current Biology","issue":"3","article_type":"original","date_published":"2020-02-03T00:00:00Z","month":"02","language":[{"iso":"eng"}],"scopus_import":"1","publisher":"Cell Press","department":[{"_id":"JiFr"},{"_id":"EvBe"}],"has_accepted_license":"1","date_created":"2020-02-02T23:01:00Z","file":[{"file_size":5360135,"file_name":"2020_CurrentBiology_Tan.pdf","checksum":"16f7d51fe28f91c21e4896a2028df40b","date_created":"2020-09-22T09:51:28Z","access_level":"open_access","date_updated":"2020-09-22T09:51:28Z","success":1,"relation":"main_file","content_type":"application/pdf","creator":"dernst","file_id":"8555"}]},{"abstract":[{"text":"Protein abundance and localization at the plasma membrane (PM) shapes plant development and mediates adaptation to changing environmental conditions. It is regulated by ubiquitination, a post-translational modification crucial for the proper sorting of endocytosed PM proteins to the vacuole for subsequent degradation. To understand the significance and the variety of roles played by this reversible modification, the function of ubiquitin receptors, which translate the ubiquitin signature into a cellular response, needs to be elucidated. In this study, we show that TOL (TOM1-like) proteins function in plants as multivalent ubiquitin receptors, governing ubiquitinated cargo delivery to the vacuole via the conserved Endosomal Sorting Complex Required for Transport (ESCRT) pathway. TOL2 and TOL6 interact with components of the ESCRT machinery and bind to K63-linked ubiquitin via two tandemly arranged conserved ubiquitin-binding domains. Mutation of these domains results not only in a loss of ubiquitin binding but also altered localization, abolishing TOL6 ubiquitin receptor activity. Function and localization of TOL6 is itself regulated by ubiquitination, whereby TOL6 ubiquitination potentially modulates degradation of PM-localized cargoes, assisting in the fine-tuning of the delicate interplay between protein recycling and downregulation. Taken together, our findings demonstrate the function and regulation of a ubiquitin receptor that mediates vacuolar degradation of PM proteins in higher plants.","lang":"eng"}],"author":[{"last_name":"Moulinier-Anzola","full_name":"Moulinier-Anzola, Jeanette","first_name":"Jeanette"},{"first_name":"Maximilian","full_name":"Schwihla, Maximilian","last_name":"Schwihla"},{"full_name":"De-Araújo, Lucinda","last_name":"De-Araújo","first_name":"Lucinda"},{"id":"45DF286A-F248-11E8-B48F-1D18A9856A87","first_name":"Christina","full_name":"Artner, Christina","last_name":"Artner"},{"full_name":"Jörg, Lisa","last_name":"Jörg","first_name":"Lisa"},{"first_name":"Nataliia","last_name":"Konstantinova","full_name":"Konstantinova, Nataliia"},{"first_name":"Christian","last_name":"Luschnig","full_name":"Luschnig, Christian"},{"last_name":"Korbei","full_name":"Korbei, Barbara","first_name":"Barbara"}],"keyword":["Plant Science","Molecular Biology"],"publication_status":"published","citation":{"ieee":"J. Moulinier-Anzola <i>et al.</i>, “TOLs function as ubiquitin receptors in the early steps of the ESCRT pathway in higher plants,” <i>Molecular Plant</i>, vol. 13, no. 5. Elsevier, pp. 717–731, 2020.","apa":"Moulinier-Anzola, J., Schwihla, M., De-Araújo, L., Artner, C., Jörg, L., Konstantinova, N., … Korbei, B. (2020). TOLs function as ubiquitin receptors in the early steps of the ESCRT pathway in higher plants. <i>Molecular Plant</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.molp.2020.02.012\">https://doi.org/10.1016/j.molp.2020.02.012</a>","chicago":"Moulinier-Anzola, Jeanette, Maximilian Schwihla, Lucinda De-Araújo, Christina Artner, Lisa Jörg, Nataliia Konstantinova, Christian Luschnig, and Barbara Korbei. “TOLs Function as Ubiquitin Receptors in the Early Steps of the ESCRT Pathway in Higher Plants.” <i>Molecular Plant</i>. Elsevier, 2020. <a href=\"https://doi.org/10.1016/j.molp.2020.02.012\">https://doi.org/10.1016/j.molp.2020.02.012</a>.","mla":"Moulinier-Anzola, Jeanette, et al. “TOLs Function as Ubiquitin Receptors in the Early Steps of the ESCRT Pathway in Higher Plants.” <i>Molecular Plant</i>, vol. 13, no. 5, Elsevier, 2020, pp. 717–31, doi:<a href=\"https://doi.org/10.1016/j.molp.2020.02.012\">10.1016/j.molp.2020.02.012</a>.","ama":"Moulinier-Anzola J, Schwihla M, De-Araújo L, et al. TOLs function as ubiquitin receptors in the early steps of the ESCRT pathway in higher plants. <i>Molecular Plant</i>. 2020;13(5):717-731. doi:<a href=\"https://doi.org/10.1016/j.molp.2020.02.012\">10.1016/j.molp.2020.02.012</a>","ista":"Moulinier-Anzola J, Schwihla M, De-Araújo L, Artner C, Jörg L, Konstantinova N, Luschnig C, Korbei B. 2020. TOLs function as ubiquitin receptors in the early steps of the ESCRT pathway in higher plants. Molecular Plant. 13(5), 717–731.","short":"J. Moulinier-Anzola, M. Schwihla, L. De-Araújo, C. Artner, L. Jörg, N. Konstantinova, C. Luschnig, B. Korbei, Molecular Plant 13 (2020) 717–731."},"pmid":1,"_id":"15037","publication_identifier":{"issn":["1674-2052"]},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","quality_controlled":"1","oa_version":"Published Version","date_updated":"2024-02-28T12:41:52Z","oa":1,"volume":13,"article_processing_charge":"No","title":"TOLs function as ubiquitin receptors in the early steps of the ESCRT pathway in higher plants","external_id":{"pmid":["32087370"]},"year":"2020","doi":"10.1016/j.molp.2020.02.012","ddc":["580"],"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"intvolume":"        13","status":"public","day":"04","type":"journal_article","issue":"5","publication":"Molecular Plant","file_date_updated":"2024-02-28T12:39:56Z","page":"717-731","publisher":"Elsevier","language":[{"iso":"eng"}],"month":"05","date_published":"2020-05-04T00:00:00Z","article_type":"original","file":[{"access_level":"open_access","date_updated":"2024-02-28T12:39:56Z","checksum":"c538a5008f7827f62d17d40a3bfabe65","date_created":"2024-02-28T12:39:56Z","file_size":3089212,"file_name":"2020_MolecularPlant_MoulinierAnzola.pdf","creator":"dernst","file_id":"15038","relation":"main_file","content_type":"application/pdf","success":1}],"date_created":"2024-02-28T08:55:56Z","has_accepted_license":"1","department":[{"_id":"EvBe"}]},{"file_date_updated":"2021-02-18T10:23:59Z","publication":"Plant Communications","issue":"3","type":"journal_article","day":"11","status":"public","intvolume":"         1","department":[{"_id":"EvBe"}],"has_accepted_license":"1","date_created":"2021-02-18T10:18:43Z","file":[{"creator":"dernst","file_id":"9161","content_type":"application/pdf","relation":"main_file","success":1,"date_updated":"2021-02-18T10:23:59Z","access_level":"open_access","date_created":"2021-02-18T10:23:59Z","checksum":"785b266d82a94b007cf40dbbe7c4847e","file_name":"2020_PlantComm_Semeradova.pdf","file_size":840289}],"date_published":"2020-05-11T00:00:00Z","article_type":"original","month":"05","language":[{"iso":"eng"}],"scopus_import":"1","publisher":"Elsevier","article_processing_charge":"No","volume":1,"oa":1,"date_updated":"2024-03-25T23:30:26Z","oa_version":"Published Version","project":[{"grant_number":"24746","_id":"261821BC-B435-11E9-9278-68D0E5697425","name":"Molecular mechanisms of the cytokinin regulated endomembrane trafficking to coordinate plant organogenesis."},{"grant_number":"ALTF710-2016","_id":"253E54C8-B435-11E9-9278-68D0E5697425","name":"Molecular mechanism of auxindriven formative divisions delineating lateral root organogenesis in plants"}],"quality_controlled":"1","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","acknowledgement":"H.S. is the recipient of a DOC Fellowship of the Austrian Academy of Sciences at the Institute of Science and Technology, Austria. J.C.M. is the recipient of an EMBO Long-Term Fellowship (ALTF number 710-2016). We would like to thank Jiri Friml and Carina Baskett for critical reading of the manuscript and Shutang Tan and Maciek Adamowski for helpful discussions. No conflict of interest declared.","publication_identifier":{"issn":["2590-3462"]},"_id":"9160","pmid":1,"citation":{"ama":"Semerádová H, Montesinos López JC, Benková E. All roads lead to auxin: Post-translational regulation of auxin transport by multiple hormonal pathways. <i>Plant Communications</i>. 2020;1(3). doi:<a href=\"https://doi.org/10.1016/j.xplc.2020.100048\">10.1016/j.xplc.2020.100048</a>","mla":"Semerádová, Hana, et al. “All Roads Lead to Auxin: Post-Translational Regulation of Auxin Transport by Multiple Hormonal Pathways.” <i>Plant Communications</i>, vol. 1, no. 3, 100048, Elsevier, 2020, doi:<a href=\"https://doi.org/10.1016/j.xplc.2020.100048\">10.1016/j.xplc.2020.100048</a>.","short":"H. Semerádová, J.C. Montesinos López, E. Benková, Plant Communications 1 (2020).","ista":"Semerádová H, Montesinos López JC, Benková E. 2020. All roads lead to auxin: Post-translational regulation of auxin transport by multiple hormonal pathways. Plant Communications. 1(3), 100048.","apa":"Semerádová, H., Montesinos López, J. C., &#38; Benková, E. (2020). All roads lead to auxin: Post-translational regulation of auxin transport by multiple hormonal pathways. <i>Plant Communications</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.xplc.2020.100048\">https://doi.org/10.1016/j.xplc.2020.100048</a>","ieee":"H. Semerádová, J. C. Montesinos López, and E. Benková, “All roads lead to auxin: Post-translational regulation of auxin transport by multiple hormonal pathways,” <i>Plant Communications</i>, vol. 1, no. 3. Elsevier, 2020.","chicago":"Semerádová, Hana, Juan C Montesinos López, and Eva Benková. “All Roads Lead to Auxin: Post-Translational Regulation of Auxin Transport by Multiple Hormonal Pathways.” <i>Plant Communications</i>. Elsevier, 2020. <a href=\"https://doi.org/10.1016/j.xplc.2020.100048\">https://doi.org/10.1016/j.xplc.2020.100048</a>."},"publication_status":"published","author":[{"id":"42FE702E-F248-11E8-B48F-1D18A9856A87","first_name":"Hana","last_name":"Semeradova","full_name":"Semeradova, Hana"},{"full_name":"Montesinos López, Juan C","last_name":"Montesinos López","orcid":"0000-0001-9179-6099","first_name":"Juan C","id":"310A8E3E-F248-11E8-B48F-1D18A9856A87"},{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","first_name":"Eva","orcid":"0000-0002-8510-9739","full_name":"Benková, Eva","last_name":"Benková"}],"abstract":[{"lang":"eng","text":"Auxin is a key hormonal regulator, that governs plant growth and development in concert with other hormonal pathways. The unique feature of auxin is its polar, cell-to-cell transport that leads to the formation of local auxin maxima and gradients, which coordinate initiation and patterning of plant organs. The molecular machinery mediating polar auxin transport is one of the important points of interaction with other hormones. Multiple hormonal pathways converge at the regulation of auxin transport and form a regulatory network that integrates various developmental and environmental inputs to steer plant development. In this review, we discuss recent advances in understanding the mechanisms that underlie regulation of polar auxin transport by multiple hormonal pathways. Specifically, we focus on the post-translational mechanisms that contribute to fine-tuning of the abundance and polarity of auxin transporters at the plasma membrane and thereby enable rapid modification of the auxin flow to coordinate plant growth and development."}],"article_number":"100048","isi":1,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)"},"ddc":["580"],"related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"10135"}]},"year":"2020","doi":"10.1016/j.xplc.2020.100048","title":"All roads lead to auxin: Post-translational regulation of auxin transport by multiple hormonal pathways","external_id":{"isi":["000654052800010"],"pmid":["33367243"]}},{"month":"09","article_type":"original","date_published":"2019-09-12T00:00:00Z","publisher":"The Company of Biologists","scopus_import":"1","language":[{"iso":"eng"}],"department":[{"_id":"EvBe"}],"date_created":"2019-09-22T22:00:36Z","day":"12","type":"journal_article","intvolume":"       146","status":"public","issue":"17","publication":"Development","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"}],"year":"2019","doi":"10.1242/dev.175919","ec_funded":1,"title":"Root gravity response module guides differential growth determining both root bending and apical hook formation in Arabidopsis","external_id":{"isi":["000486297400011"],"pmid":["31391194"]},"article_number":"dev175919","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1242/dev.175919"}],"isi":1,"publication_status":"published","citation":{"ista":"Zhu Q, Gallemi M, Pospíšil J, Žádníková P, Strnad M, Benková E. 2019. Root gravity response module guides differential growth determining both root bending and apical hook formation in Arabidopsis. Development. 146(17), dev175919.","short":"Q. Zhu, M. Gallemi, J. Pospíšil, P. Žádníková, M. Strnad, E. Benková, Development 146 (2019).","mla":"Zhu, Qiang, et al. “Root Gravity Response Module Guides Differential Growth Determining Both Root Bending and Apical Hook Formation in Arabidopsis.” <i>Development</i>, vol. 146, no. 17, dev175919, The Company of Biologists, 2019, doi:<a href=\"https://doi.org/10.1242/dev.175919\">10.1242/dev.175919</a>.","ama":"Zhu Q, Gallemi M, Pospíšil J, Žádníková P, Strnad M, Benková E. Root gravity response module guides differential growth determining both root bending and apical hook formation in Arabidopsis. <i>Development</i>. 2019;146(17). doi:<a href=\"https://doi.org/10.1242/dev.175919\">10.1242/dev.175919</a>","chicago":"Zhu, Qiang, Marçal Gallemi, Jiří Pospíšil, Petra Žádníková, Miroslav Strnad, and Eva Benková. “Root Gravity Response Module Guides Differential Growth Determining Both Root Bending and Apical Hook Formation in Arabidopsis.” <i>Development</i>. The Company of Biologists, 2019. <a href=\"https://doi.org/10.1242/dev.175919\">https://doi.org/10.1242/dev.175919</a>.","ieee":"Q. Zhu, M. Gallemi, J. Pospíšil, P. Žádníková, M. Strnad, and E. Benková, “Root gravity response module guides differential growth determining both root bending and apical hook formation in Arabidopsis,” <i>Development</i>, vol. 146, no. 17. The Company of Biologists, 2019.","apa":"Zhu, Q., Gallemi, M., Pospíšil, J., Žádníková, P., Strnad, M., &#38; Benková, E. (2019). Root gravity response module guides differential growth determining both root bending and apical hook formation in Arabidopsis. <i>Development</i>. The Company of Biologists. <a href=\"https://doi.org/10.1242/dev.175919\">https://doi.org/10.1242/dev.175919</a>"},"abstract":[{"lang":"eng","text":"The apical hook is a transiently formed structure that plays a protective role when the germinating seedling penetrates through the soil towards the surface. Crucial for proper bending is the local auxin maxima, which defines the concave (inner) side of the hook curvature. As no sign of asymmetric auxin distribution has been reported in embryonic hypocotyls prior to hook formation, the question of how auxin asymmetry is established in the early phases of seedling germination remains largely unanswered. Here, we analyzed the auxin distribution and expression of PIN auxin efflux carriers from early phases of germination, and show that bending of the root in response to gravity is the crucial initial cue that governs the hypocotyl bending required for apical hook formation. Importantly, polar auxin transport machinery is established gradually after germination starts as a result of tight root-hypocotyl interaction and a proper balance between abscisic acid and gibberellins."}],"author":[{"first_name":"Qiang","last_name":"Zhu","full_name":"Zhu, Qiang","id":"40A4B9E6-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0003-4675-6893","last_name":"Gallemi","full_name":"Gallemi, Marçal","first_name":"Marçal","id":"460C6802-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Pospíšil, Jiří","last_name":"Pospíšil","first_name":"Jiří"},{"first_name":"Petra","last_name":"Žádníková","full_name":"Žádníková, Petra"},{"last_name":"Strnad","full_name":"Strnad, Miroslav","first_name":"Miroslav"},{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","first_name":"Eva","orcid":"0000-0002-8510-9739","last_name":"Benková","full_name":"Benková, Eva"}],"oa":1,"date_updated":"2025-05-07T11:10:55Z","volume":146,"article_processing_charge":"No","pmid":1,"_id":"6897","publication_identifier":{"eissn":["14779129"]},"acknowledgement":"We thank Jiri Friml and Phillip Brewer for inspiring discussion and for help in preparing the manuscript. This research was supported by the Scientific Service Units (SSU) of IST-Austria through resources provided by the Bioimaging Facility\r\n(BIF), the Life Science Facility (LSF).\r\nThis work was supported by grants from the European Research Council (Starting Independent Research Grant ERC-2007-Stg- 207362-HCPO to E.B.). J.P. and M.S. received funds from European Regional Development Fund-Project ‘Centre for Experimental Plant Biology’ (No. CZ.02.1.01/0.0/0.0/16_019/0000738).","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","oa_version":"Published Version","project":[{"_id":"253FCA6A-B435-11E9-9278-68D0E5697425","name":"Hormonal cross-talk in plant organogenesis","call_identifier":"FP7","grant_number":"207362"}],"quality_controlled":"1"},{"article_processing_charge":"No","date_updated":"2023-08-30T06:55:02Z","volume":12,"oa_version":"None","quality_controlled":"1","project":[{"name":"Hormonal regulation of plant adaptive responses to environmental signals","_id":"2685A872-B435-11E9-9278-68D0E5697425"}],"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","publication_identifier":{"issn":["1674-2052","1752-9867"]},"pmid":1,"_id":"6920","citation":{"chicago":"Artner, Christina, and Eva Benková. “Ethylene and Cytokinin - Partners in Root Growth Regulation.” <i>Molecular Plant</i>. Cell Press, 2019. <a href=\"https://doi.org/10.1016/j.molp.2019.09.003\">https://doi.org/10.1016/j.molp.2019.09.003</a>.","apa":"Artner, C., &#38; Benková, E. (2019). Ethylene and cytokinin - partners in root growth regulation. <i>Molecular Plant</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.molp.2019.09.003\">https://doi.org/10.1016/j.molp.2019.09.003</a>","ieee":"C. Artner and E. Benková, “Ethylene and cytokinin - partners in root growth regulation,” <i>Molecular Plant</i>, vol. 12, no. 10. Cell Press, pp. 1312–1314, 2019.","short":"C. Artner, E. Benková, Molecular Plant 12 (2019) 1312–1314.","ista":"Artner C, Benková E. 2019. Ethylene and cytokinin - partners in root growth regulation. Molecular Plant. 12(10), 1312–1314.","ama":"Artner C, Benková E. Ethylene and cytokinin - partners in root growth regulation. <i>Molecular Plant</i>. 2019;12(10):1312-1314. doi:<a href=\"https://doi.org/10.1016/j.molp.2019.09.003\">10.1016/j.molp.2019.09.003</a>","mla":"Artner, Christina, and Eva Benková. “Ethylene and Cytokinin - Partners in Root Growth Regulation.” <i>Molecular Plant</i>, vol. 12, no. 10, Cell Press, 2019, pp. 1312–14, doi:<a href=\"https://doi.org/10.1016/j.molp.2019.09.003\">10.1016/j.molp.2019.09.003</a>."},"publication_status":"published","author":[{"first_name":"Christina","full_name":"Artner, Christina","last_name":"Artner","id":"45DF286A-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Eva","full_name":"Benková, Eva","last_name":"Benková","orcid":"0000-0002-8510-9739","id":"38F4F166-F248-11E8-B48F-1D18A9856A87"}],"isi":1,"year":"2019","doi":"10.1016/j.molp.2019.09.003","external_id":{"isi":["000489132500002"],"pmid":["31541740"]},"title":"Ethylene and cytokinin - partners in root growth regulation","page":"1312-1314","publication":"Molecular Plant","issue":"10","type":"journal_article","day":"07","status":"public","intvolume":"        12","department":[{"_id":"EvBe"}],"date_created":"2019-09-30T10:00:40Z","article_type":"original","date_published":"2019-10-07T00:00:00Z","month":"10","language":[{"iso":"eng"}],"scopus_import":"1","publisher":"Cell Press"},{"page":"A1-A2","issue":"12","publication":"Current Opinion in Plant Biology","status":"public","intvolume":"        52","type":"journal_article","day":"01","date_created":"2020-01-29T16:00:07Z","department":[{"_id":"EvBe"}],"language":[{"iso":"eng"}],"publisher":"Elsevier","scopus_import":"1","article_type":"letter_note","date_published":"2019-12-01T00:00:00Z","month":"12","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","quality_controlled":"1","oa_version":"None","_id":"7394","pmid":1,"publication_identifier":{"issn":["1369-5266"]},"date_updated":"2023-09-07T14:56:55Z","volume":52,"article_processing_charge":"No","author":[{"orcid":"0000-0002-8510-9739","last_name":"Benková","full_name":"Benková, Eva","first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Dagdas","full_name":"Dagdas, Yasin","first_name":"Yasin"}],"publication_status":"published","citation":{"ieee":"E. Benková and Y. Dagdas, “Editorial overview: Cell biology in the era of omics?,” <i>Current Opinion in Plant Biology</i>, vol. 52, no. 12. Elsevier, pp. A1–A2, 2019.","apa":"Benková, E., &#38; Dagdas, Y. (2019). Editorial overview: Cell biology in the era of omics? <i>Current Opinion in Plant Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.pbi.2019.11.002\">https://doi.org/10.1016/j.pbi.2019.11.002</a>","chicago":"Benková, Eva, and Yasin Dagdas. “Editorial Overview: Cell Biology in the Era of Omics?” <i>Current Opinion in Plant Biology</i>. Elsevier, 2019. <a href=\"https://doi.org/10.1016/j.pbi.2019.11.002\">https://doi.org/10.1016/j.pbi.2019.11.002</a>.","ama":"Benková E, Dagdas Y. Editorial overview: Cell biology in the era of omics? <i>Current Opinion in Plant Biology</i>. 2019;52(12):A1-A2. doi:<a href=\"https://doi.org/10.1016/j.pbi.2019.11.002\">10.1016/j.pbi.2019.11.002</a>","mla":"Benková, Eva, and Yasin Dagdas. “Editorial Overview: Cell Biology in the Era of Omics?” <i>Current Opinion in Plant Biology</i>, vol. 52, no. 12, Elsevier, 2019, pp. A1–2, doi:<a href=\"https://doi.org/10.1016/j.pbi.2019.11.002\">10.1016/j.pbi.2019.11.002</a>.","ista":"Benková E, Dagdas Y. 2019. Editorial overview: Cell biology in the era of omics? Current Opinion in Plant Biology. 52(12), A1–A2.","short":"E. Benková, Y. Dagdas, Current Opinion in Plant Biology 52 (2019) A1–A2."},"isi":1,"external_id":{"pmid":["31787165"],"isi":["000502890600001"]},"title":"Editorial overview: Cell biology in the era of omics?","year":"2019","doi":"10.1016/j.pbi.2019.11.002"},{"type":"journal_article","day":"08","status":"public","intvolume":"         5","page":"160-166","issue":"2","publication":"Nature Plants","date_published":"2019-02-08T00:00:00Z","month":"02","language":[{"iso":"eng"}],"publisher":"Springer Nature","scopus_import":"1","department":[{"_id":"JiFr"},{"_id":"EvBe"}],"date_created":"2019-02-17T22:59:21Z","publication_status":"published","citation":{"apa":"Yoshida, S., Van Der Schuren, A., Van Dop, M., Van Galen, L., Saiga, S., Adibi, M., … Weijers, D. (2019). A SOSEKI-based coordinate system interprets global polarity cues in arabidopsis. <i>Nature Plants</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41477-019-0363-6\">https://doi.org/10.1038/s41477-019-0363-6</a>","ieee":"S. Yoshida <i>et al.</i>, “A SOSEKI-based coordinate system interprets global polarity cues in arabidopsis,” <i>Nature Plants</i>, vol. 5, no. 2. Springer Nature, pp. 160–166, 2019.","chicago":"Yoshida, Saiko, Alja Van Der Schuren, Maritza Van Dop, Luc Van Galen, Shunsuke Saiga, Milad Adibi, Barbara Möller, et al. “A SOSEKI-Based Coordinate System Interprets Global Polarity Cues in Arabidopsis.” <i>Nature Plants</i>. Springer Nature, 2019. <a href=\"https://doi.org/10.1038/s41477-019-0363-6\">https://doi.org/10.1038/s41477-019-0363-6</a>.","mla":"Yoshida, Saiko, et al. “A SOSEKI-Based Coordinate System Interprets Global Polarity Cues in Arabidopsis.” <i>Nature Plants</i>, vol. 5, no. 2, Springer Nature, 2019, pp. 160–66, doi:<a href=\"https://doi.org/10.1038/s41477-019-0363-6\">10.1038/s41477-019-0363-6</a>.","ama":"Yoshida S, Van Der Schuren A, Van Dop M, et al. A SOSEKI-based coordinate system interprets global polarity cues in arabidopsis. <i>Nature Plants</i>. 2019;5(2):160-166. doi:<a href=\"https://doi.org/10.1038/s41477-019-0363-6\">10.1038/s41477-019-0363-6</a>","short":"S. Yoshida, A. Van Der Schuren, M. Van Dop, L. Van Galen, S. Saiga, M. Adibi, B. Möller, C.A. Ten Hove, P. Marhavý, R. Smith, J. Friml, D. Weijers, Nature Plants 5 (2019) 160–166.","ista":"Yoshida S, Van Der Schuren A, Van Dop M, Van Galen L, Saiga S, Adibi M, Möller B, Ten Hove CA, Marhavý P, Smith R, Friml J, Weijers D. 2019. A SOSEKI-based coordinate system interprets global polarity cues in arabidopsis. Nature Plants. 5(2), 160–166."},"author":[{"id":"2E46069C-F248-11E8-B48F-1D18A9856A87","first_name":"Saiko","full_name":"Yoshida, Saiko","last_name":"Yoshida"},{"first_name":"Alja","last_name":"Van Der Schuren","full_name":"Van Der Schuren, Alja"},{"first_name":"Maritza","full_name":"Van Dop, Maritza","last_name":"Van Dop"},{"last_name":"Van Galen","full_name":"Van Galen, Luc","first_name":"Luc"},{"last_name":"Saiga","full_name":"Saiga, Shunsuke","first_name":"Shunsuke"},{"first_name":"Milad","full_name":"Adibi, Milad","last_name":"Adibi"},{"first_name":"Barbara","last_name":"Möller","full_name":"Möller, Barbara"},{"full_name":"Ten Hove, Colette A.","last_name":"Ten Hove","first_name":"Colette A."},{"last_name":"Marhavy","full_name":"Marhavy, Peter","orcid":"0000-0001-5227-5741","first_name":"Peter","id":"3F45B078-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Richard","full_name":"Smith, Richard","last_name":"Smith"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","first_name":"Jiří"},{"last_name":"Weijers","full_name":"Weijers, Dolf","first_name":"Dolf"}],"abstract":[{"lang":"eng","text":"Multicellular development requires coordinated cell polarization relative to body axes, and translation to oriented cell division 1–3 . In plants, it is unknown how cell polarities are connected to organismal axes and translated to division. Here, we identify Arabidopsis SOSEKI proteins that integrate apical–basal and radial organismal axes to localize to polar cell edges. Localization does not depend on tissue context, requires cell wall integrity and is defined by a transferrable, protein-specific motif. A Domain of Unknown Function in SOSEKI proteins resembles the DIX oligomerization domain in the animal Dishevelled polarity regulator. The DIX-like domain self-interacts and is required for edge localization and for influencing division orientation, together with a second domain that defines the polar membrane domain. Our work shows that SOSEKI proteins locally interpret global polarity cues and can influence cell division orientation. Furthermore, this work reveals that, despite fundamental differences, cell polarity mechanisms in plants and animals converge on a similar protein domain."}],"date_updated":"2023-08-24T14:46:47Z","oa":1,"volume":5,"article_processing_charge":"No","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","project":[{"call_identifier":"FP7","_id":"25681D80-B435-11E9-9278-68D0E5697425","name":"International IST Postdoc Fellowship Programme","grant_number":"291734"}],"oa_version":"Submitted Version","quality_controlled":"1","_id":"6023","ec_funded":1,"year":"2019","doi":"10.1038/s41477-019-0363-6","title":"A SOSEKI-based coordinate system interprets global polarity cues in arabidopsis","external_id":{"isi":["000460479600014"]},"main_file_link":[{"url":"https://www.biorxiv.org/content/10.1101/479113v1.abstract","open_access":"1"}],"isi":1},{"doi":"10.1016/j.cell.2019.04.015","year":"2019","acknowledged_ssus":[{"_id":"Bio"}],"ec_funded":1,"title":"Re-activation of stem cell pathways for pattern restoration in plant wound healing","external_id":{"isi":["000466843000015"],"pmid":["31051107"]},"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"isi":1,"related_material":{"record":[{"id":"9992","relation":"dissertation_contains","status":"public"}],"link":[{"url":"https://ist.ac.at/en/news/specialized-plant-cells-regain-stem-cell-features-to-heal-wounds/","description":"News on IST Homepage","relation":"press_release"}]},"ddc":["570"],"citation":{"chicago":"Marhavá, Petra, Lukas Hörmayer, Saiko Yoshida, Peter Marhavý, Eva Benková, and Jiří Friml. “Re-Activation of Stem Cell Pathways for Pattern Restoration in Plant Wound Healing.” <i>Cell</i>. Elsevier, 2019. <a href=\"https://doi.org/10.1016/j.cell.2019.04.015\">https://doi.org/10.1016/j.cell.2019.04.015</a>.","apa":"Marhavá, P., Hörmayer, L., Yoshida, S., Marhavý, P., Benková, E., &#38; Friml, J. (2019). Re-activation of stem cell pathways for pattern restoration in plant wound healing. <i>Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cell.2019.04.015\">https://doi.org/10.1016/j.cell.2019.04.015</a>","ieee":"P. Marhavá, L. Hörmayer, S. Yoshida, P. Marhavý, E. Benková, and J. Friml, “Re-activation of stem cell pathways for pattern restoration in plant wound healing,” <i>Cell</i>, vol. 177, no. 4. Elsevier, p. 957–969.e13, 2019.","short":"P. Marhavá, L. Hörmayer, S. Yoshida, P. Marhavý, E. Benková, J. Friml, Cell 177 (2019) 957–969.e13.","ista":"Marhavá P, Hörmayer L, Yoshida S, Marhavý P, Benková E, Friml J. 2019. Re-activation of stem cell pathways for pattern restoration in plant wound healing. Cell. 177(4), 957–969.e13.","mla":"Marhavá, Petra, et al. “Re-Activation of Stem Cell Pathways for Pattern Restoration in Plant Wound Healing.” <i>Cell</i>, vol. 177, no. 4, Elsevier, 2019, p. 957–969.e13, doi:<a href=\"https://doi.org/10.1016/j.cell.2019.04.015\">10.1016/j.cell.2019.04.015</a>.","ama":"Marhavá P, Hörmayer L, Yoshida S, Marhavý P, Benková E, Friml J. Re-activation of stem cell pathways for pattern restoration in plant wound healing. <i>Cell</i>. 2019;177(4):957-969.e13. doi:<a href=\"https://doi.org/10.1016/j.cell.2019.04.015\">10.1016/j.cell.2019.04.015</a>"},"publication_status":"published","abstract":[{"text":"A process of restorative patterning in plant roots correctly replaces eliminated cells to heal local injuries despite the absence of cell migration, which underpins wound healing in animals. \r\n\r\nPatterning in plants relies on oriented cell divisions and acquisition of specific cell identities. Plants regularly endure wounds caused by abiotic or biotic environmental stimuli and have developed extraordinary abilities to restore their tissues after injuries. Here, we provide insight into a mechanism of restorative patterning that repairs tissues after wounding. Laser-assisted elimination of different cells in Arabidopsis root combined with live-imaging tracking during vertical growth allowed analysis of the regeneration processes in vivo. Specifically, the cells adjacent to the inner side of the injury re-activated their stem cell transcriptional programs. They accelerated their progression through cell cycle, coordinately changed the cell division orientation, and ultimately acquired de novo the correct cell fates to replace missing cells. These observations highlight existence of unknown intercellular positional signaling and demonstrate the capability of specified cells to re-acquire stem cell programs as a crucial part of the plant-specific mechanism of wound healing.","lang":"eng"}],"author":[{"first_name":"Petra","last_name":"Marhavá","full_name":"Marhavá, Petra","id":"44E59624-F248-11E8-B48F-1D18A9856A87"},{"id":"2EEE7A2A-F248-11E8-B48F-1D18A9856A87","first_name":"Lukas","full_name":"Hörmayer, Lukas","last_name":"Hörmayer","orcid":"0000-0001-8295-2926"},{"id":"2E46069C-F248-11E8-B48F-1D18A9856A87","full_name":"Yoshida, Saiko","last_name":"Yoshida","first_name":"Saiko"},{"id":"3F45B078-F248-11E8-B48F-1D18A9856A87","full_name":"Marhavy, Peter","last_name":"Marhavy","orcid":"0000-0001-5227-5741","first_name":"Peter"},{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8510-9739","last_name":"Benková","full_name":"Benková, Eva","first_name":"Eva"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří","last_name":"Friml","first_name":"Jiří"}],"article_processing_charge":"No","oa":1,"volume":177,"date_updated":"2024-03-25T23:30:06Z","publication_identifier":{"issn":["00928674"],"eissn":["10974172"]},"_id":"6351","pmid":1,"project":[{"grant_number":"742985","call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","_id":"261099A6-B435-11E9-9278-68D0E5697425"}],"oa_version":"Published Version","quality_controlled":"1","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","month":"05","date_published":"2019-05-02T00:00:00Z","scopus_import":"1","publisher":"Elsevier","language":[{"iso":"eng"}],"has_accepted_license":"1","department":[{"_id":"JiFr"},{"_id":"EvBe"}],"date_created":"2019-04-28T21:59:14Z","file":[{"checksum":"4ceba04a96a74f5092ec3ce2c579a0c7","date_created":"2019-05-13T06:12:45Z","file_size":10272032,"file_name":"2019_Cell_Marhava.pdf","access_level":"open_access","date_updated":"2020-07-14T12:47:28Z","creator":"dernst","file_id":"6411","relation":"main_file","content_type":"application/pdf"}],"day":"02","type":"journal_article","intvolume":"       177","status":"public","publication":"Cell","issue":"4","page":"957-969.e13","file_date_updated":"2020-07-14T12:47:28Z"},{"department":[{"_id":"EvBe"}],"has_accepted_license":"1","date_created":"2018-12-11T11:45:34Z","file":[{"content_type":"application/pdf","relation":"main_file","creator":"dernst","file_id":"7834","file_name":"2018_PlantMolecBio_Dokladal.pdf","file_size":1150679,"date_created":"2020-05-14T12:23:08Z","checksum":"451ae47616e6af2533099f596b2a47fb","date_updated":"2020-07-14T12:45:45Z","access_level":"open_access"}],"article_type":"original","date_published":"2018-06-12T00:00:00Z","month":"06","language":[{"iso":"eng"}],"publisher":"Springer","scopus_import":"1","page":"407 - 420","file_date_updated":"2020-07-14T12:45:45Z","issue":"5","publication":"Plant Molecular Biology","type":"journal_article","day":"12","status":"public","intvolume":"        97","isi":1,"ddc":["580"],"doi":"10.1007/s11103-018-0747-4","year":"2018","title":"An armadillo-domain protein participates in a telomerase interaction network","external_id":{"isi":["000438981700009"]},"volume":97,"publist_id":"7625","oa":1,"date_updated":"2023-09-08T13:21:05Z","article_processing_charge":"No","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","oa_version":"Submitted Version","quality_controlled":"1","_id":"277","publication_status":"published","citation":{"ama":"Dokládal L, Benková E, Honys D, et al. An armadillo-domain protein participates in a telomerase interaction network. <i>Plant Molecular Biology</i>. 2018;97(5):407-420. doi:<a href=\"https://doi.org/10.1007/s11103-018-0747-4\">10.1007/s11103-018-0747-4</a>","mla":"Dokládal, Ladislav, et al. “An Armadillo-Domain Protein Participates in a Telomerase Interaction Network.” <i>Plant Molecular Biology</i>, vol. 97, no. 5, Springer, 2018, pp. 407–20, doi:<a href=\"https://doi.org/10.1007/s11103-018-0747-4\">10.1007/s11103-018-0747-4</a>.","ista":"Dokládal L, Benková E, Honys D, Dupláková N, Lee L, Gelvin S, Sýkorová E. 2018. An armadillo-domain protein participates in a telomerase interaction network. Plant Molecular Biology. 97(5), 407–420.","short":"L. Dokládal, E. Benková, D. Honys, N. Dupláková, L. Lee, S. Gelvin, E. Sýkorová, Plant Molecular Biology 97 (2018) 407–420.","apa":"Dokládal, L., Benková, E., Honys, D., Dupláková, N., Lee, L., Gelvin, S., &#38; Sýkorová, E. (2018). An armadillo-domain protein participates in a telomerase interaction network. <i>Plant Molecular Biology</i>. Springer. <a href=\"https://doi.org/10.1007/s11103-018-0747-4\">https://doi.org/10.1007/s11103-018-0747-4</a>","ieee":"L. Dokládal <i>et al.</i>, “An armadillo-domain protein participates in a telomerase interaction network,” <i>Plant Molecular Biology</i>, vol. 97, no. 5. Springer, pp. 407–420, 2018.","chicago":"Dokládal, Ladislav, Eva Benková, David Honys, Nikoleta Dupláková, Lan Lee, Stanton Gelvin, and Eva Sýkorová. “An Armadillo-Domain Protein Participates in a Telomerase Interaction Network.” <i>Plant Molecular Biology</i>. Springer, 2018. <a href=\"https://doi.org/10.1007/s11103-018-0747-4\">https://doi.org/10.1007/s11103-018-0747-4</a>."},"author":[{"full_name":"Dokládal, Ladislav","last_name":"Dokládal","first_name":"Ladislav"},{"orcid":"0000-0002-8510-9739","last_name":"Benková","full_name":"Benková, Eva","first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Honys","full_name":"Honys, David","first_name":"David"},{"first_name":"Nikoleta","last_name":"Dupláková","full_name":"Dupláková, Nikoleta"},{"first_name":"Lan","last_name":"Lee","full_name":"Lee, Lan"},{"last_name":"Gelvin","full_name":"Gelvin, Stanton","first_name":"Stanton"},{"full_name":"Sýkorová, Eva","last_name":"Sýkorová","first_name":"Eva"}],"abstract":[{"lang":"eng","text":"Arabidopsis and human ARM protein interact with telomerase. Deregulated mRNA levels of DNA repair and ribosomal protein genes in an Arabidopsis arm mutant suggest non-telomeric ARM function. The human homolog ARMC6 interacts with hTRF2. Abstract: Telomerase maintains telomeres and has proposed non-telomeric functions. We previously identified interaction of the C-terminal domain of Arabidopsis telomerase reverse transcriptase (AtTERT) with an armadillo/β-catenin-like repeat (ARM) containing protein. Here we explore protein–protein interactions of the ARM protein, AtTERT domains, POT1a, TRF-like family and SMH family proteins, and the chromatin remodeling protein CHR19 using bimolecular fluorescence complementation (BiFC), yeast two-hybrid (Y2H) analysis, and co-immunoprecipitation. The ARM protein interacts with both the N- and C-terminal domains of AtTERT in different cellular compartments. ARM interacts with CHR19 and TRF-like I family proteins that also bind AtTERT directly or through interaction with POT1a. The putative human ARM homolog co-precipitates telomerase activity and interacts with hTRF2 protein in vitro. Analysis of Arabidopsis arm mutants shows no obvious changes in telomere length or telomerase activity, suggesting that ARM is not essential for telomere maintenance. The observed interactions with telomerase and Myb-like domain proteins (TRF-like family I) may therefore reflect possible non-telomeric functions. Transcript levels of several DNA repair and ribosomal genes are affected in arm mutants, and ARM, likely in association with other proteins, suppressed expression of XRCC3 and RPSAA promoter constructs in luciferase reporter assays. In conclusion, ARM can participate in non-telomeric functions of telomerase, and can also perform its own telomerase-independent functions."}]},{"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"article_number":"8754","isi":1,"ddc":["570"],"year":"2018","doi":"10.1038/s41598-018-27080-2","external_id":{"isi":["000434640800008"]},"title":"Mutations in blind cavefish target the light regulated circadian clock gene period 2","article_processing_charge":"No","publist_id":"7616","volume":8,"oa":1,"date_updated":"2023-09-13T08:59:27Z","_id":"283","quality_controlled":"1","oa_version":"Published Version","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","citation":{"chicago":"Ceinos, Rosa Maria, Elena Frigato, Cristina Pagano, Nadine Frohlich, Pietro Negrini, Nicola Cavallari, Daniela Vallone, Silvia Fuselli, Cristiano Bertolucci, and Nicholas S Foulkes. “Mutations in Blind Cavefish Target the Light Regulated Circadian Clock Gene Period 2.” <i>Scientific Reports</i>. Nature Publishing Group, 2018. <a href=\"https://doi.org/10.1038/s41598-018-27080-2\">https://doi.org/10.1038/s41598-018-27080-2</a>.","apa":"Ceinos, R. M., Frigato, E., Pagano, C., Frohlich, N., Negrini, P., Cavallari, N., … Foulkes, N. S. (2018). Mutations in blind cavefish target the light regulated circadian clock gene period 2. <i>Scientific Reports</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/s41598-018-27080-2\">https://doi.org/10.1038/s41598-018-27080-2</a>","ieee":"R. M. Ceinos <i>et al.</i>, “Mutations in blind cavefish target the light regulated circadian clock gene period 2,” <i>Scientific Reports</i>, vol. 8, no. 1. Nature Publishing Group, 2018.","ista":"Ceinos RM, Frigato E, Pagano C, Frohlich N, Negrini P, Cavallari N, Vallone D, Fuselli S, Bertolucci C, Foulkes NS. 2018. Mutations in blind cavefish target the light regulated circadian clock gene period 2. Scientific Reports. 8(1), 8754.","short":"R.M. Ceinos, E. Frigato, C. Pagano, N. Frohlich, P. Negrini, N. Cavallari, D. Vallone, S. Fuselli, C. Bertolucci, N.S. Foulkes, Scientific Reports 8 (2018).","mla":"Ceinos, Rosa Maria, et al. “Mutations in Blind Cavefish Target the Light Regulated Circadian Clock Gene Period 2.” <i>Scientific Reports</i>, vol. 8, no. 1, 8754, Nature Publishing Group, 2018, doi:<a href=\"https://doi.org/10.1038/s41598-018-27080-2\">10.1038/s41598-018-27080-2</a>.","ama":"Ceinos RM, Frigato E, Pagano C, et al. Mutations in blind cavefish target the light regulated circadian clock gene period 2. <i>Scientific Reports</i>. 2018;8(1). doi:<a href=\"https://doi.org/10.1038/s41598-018-27080-2\">10.1038/s41598-018-27080-2</a>"},"publication_status":"published","abstract":[{"text":"Light represents the principal signal driving circadian clock entrainment. However, how light influences the evolution of the clock remains poorly understood. The cavefish Phreatichthys andruzzii represents a fascinating model to explore how evolution under extreme aphotic conditions shapes the circadian clock, since in this species the clock is unresponsive to light. We have previously demonstrated that loss-of-function mutations targeting non-visual opsins contribute in part to this blind clock phenotype. Here, we have compared orthologs of two core clock genes that play a key role in photic entrainment, cry1a and per2, in both zebrafish and P. andruzzii. We encountered aberrantly spliced variants for the P. andruzzii per2 transcript. The most abundant transcript encodes a truncated protein lacking the C-terminal Cry binding domain and incorporating an intronic, transposon-derived coding sequence. We demonstrate that the transposon insertion leads to a predominantly cytoplasmic localization of the cavefish Per2 protein in contrast to the zebrafish ortholog which is distributed in both the nucleus and cytoplasm. Thus, it seems that during evolution in complete darkness, the photic entrainment pathway of the circadian clock has been subject to mutation at multiple levels, extending from opsin photoreceptors to nuclear effectors.","lang":"eng"}],"author":[{"first_name":"Rosa Maria","full_name":"Ceinos, Rosa Maria","last_name":"Ceinos"},{"first_name":"Elena","last_name":"Frigato","full_name":"Frigato, Elena"},{"first_name":"Cristina","last_name":"Pagano","full_name":"Pagano, Cristina"},{"first_name":"Nadine","last_name":"Frohlich","full_name":"Frohlich, Nadine"},{"full_name":"Negrini, Pietro","last_name":"Negrini","first_name":"Pietro"},{"first_name":"Nicola","last_name":"Cavallari","full_name":"Cavallari, Nicola","id":"457160E6-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Vallone","full_name":"Vallone, Daniela","first_name":"Daniela"},{"first_name":"Silvia","full_name":"Fuselli, Silvia","last_name":"Fuselli"},{"first_name":"Cristiano","full_name":"Bertolucci, Cristiano","last_name":"Bertolucci"},{"first_name":"Nicholas S","last_name":"Foulkes","full_name":"Foulkes, Nicholas S"}],"has_accepted_license":"1","department":[{"_id":"EvBe"}],"file":[{"file_id":"5707","creator":"dernst","relation":"main_file","content_type":"application/pdf","access_level":"open_access","date_updated":"2020-07-14T12:45:49Z","checksum":"9c3942d772f84f3df032ffde0ed9a8ea","date_created":"2018-12-17T13:04:46Z","file_size":1855324,"file_name":"2018_ScientificReports_Ceinos.pdf"}],"date_created":"2018-12-11T11:45:36Z","month":"06","date_published":"2018-06-08T00:00:00Z","scopus_import":"1","publisher":"Nature Publishing Group","language":[{"iso":"eng"}],"publication":"Scientific Reports","issue":"1","file_date_updated":"2020-07-14T12:45:49Z","day":"08","type":"journal_article","intvolume":"         8","status":"public"},{"has_accepted_license":"1","department":[{"_id":"JiFr"},{"_id":"EvBe"}],"file":[{"file_id":"5714","creator":"dernst","relation":"main_file","content_type":"application/pdf","access_level":"open_access","date_updated":"2020-07-14T12:45:20Z","checksum":"266b03f4fb8198e83141617aaa99dcab","date_created":"2018-12-17T15:38:56Z","file_size":2413876,"file_name":"2018_ScientificReports_Grones.pdf"}],"date_created":"2018-12-11T11:45:06Z","month":"07","date_published":"2018-07-06T00:00:00Z","publisher":"Springer","scopus_import":"1","language":[{"iso":"eng"}],"issue":"1","publication":"Scientific Reports","file_date_updated":"2020-07-14T12:45:20Z","day":"06","type":"journal_article","intvolume":"         8","status":"public","tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"isi":1,"article_number":"10279","related_material":{"record":[{"id":"8822","relation":"dissertation_contains","status":"public"}]},"ddc":["581"],"year":"2018","doi":"10.1038/s41598-018-28188-1","ec_funded":1,"external_id":{"isi":["000437673200053"]},"title":"PID/WAG-mediated phosphorylation of the Arabidopsis PIN3 auxin transporter mediates polarity switches during gravitropism","volume":8,"publist_id":"7729","date_updated":"2025-05-07T11:12:31Z","oa":1,"article_processing_charge":"No","_id":"191","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","project":[{"grant_number":"282300","_id":"25716A02-B435-11E9-9278-68D0E5697425","name":"Polarity and subcellular dynamics in plants","call_identifier":"FP7"},{"call_identifier":"H2020","_id":"261099A6-B435-11E9-9278-68D0E5697425","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","grant_number":"742985"}],"quality_controlled":"1","oa_version":"Published Version","publication_status":"published","citation":{"chicago":"Grones, Peter, Melinda F Abas, Jakub Hajny, Angharad Jones, Sascha Waidmann, Jürgen Kleine Vehn, and Jiří Friml. “PID/WAG-Mediated Phosphorylation of the Arabidopsis PIN3 Auxin Transporter Mediates Polarity Switches during Gravitropism.” <i>Scientific Reports</i>. Springer, 2018. <a href=\"https://doi.org/10.1038/s41598-018-28188-1\">https://doi.org/10.1038/s41598-018-28188-1</a>.","apa":"Grones, P., Abas, M. F., Hajny, J., Jones, A., Waidmann, S., Kleine Vehn, J., &#38; Friml, J. (2018). PID/WAG-mediated phosphorylation of the Arabidopsis PIN3 auxin transporter mediates polarity switches during gravitropism. <i>Scientific Reports</i>. Springer. <a href=\"https://doi.org/10.1038/s41598-018-28188-1\">https://doi.org/10.1038/s41598-018-28188-1</a>","ieee":"P. Grones <i>et al.</i>, “PID/WAG-mediated phosphorylation of the Arabidopsis PIN3 auxin transporter mediates polarity switches during gravitropism,” <i>Scientific Reports</i>, vol. 8, no. 1. Springer, 2018.","ista":"Grones P, Abas MF, Hajny J, Jones A, Waidmann S, Kleine Vehn J, Friml J. 2018. PID/WAG-mediated phosphorylation of the Arabidopsis PIN3 auxin transporter mediates polarity switches during gravitropism. Scientific Reports. 8(1), 10279.","short":"P. Grones, M.F. Abas, J. Hajny, A. Jones, S. Waidmann, J. Kleine Vehn, J. Friml, Scientific Reports 8 (2018).","mla":"Grones, Peter, et al. “PID/WAG-Mediated Phosphorylation of the Arabidopsis PIN3 Auxin Transporter Mediates Polarity Switches during Gravitropism.” <i>Scientific Reports</i>, vol. 8, no. 1, 10279, Springer, 2018, doi:<a href=\"https://doi.org/10.1038/s41598-018-28188-1\">10.1038/s41598-018-28188-1</a>.","ama":"Grones P, Abas MF, Hajny J, et al. PID/WAG-mediated phosphorylation of the Arabidopsis PIN3 auxin transporter mediates polarity switches during gravitropism. <i>Scientific Reports</i>. 2018;8(1). doi:<a href=\"https://doi.org/10.1038/s41598-018-28188-1\">10.1038/s41598-018-28188-1</a>"},"abstract":[{"text":"Intercellular distribution of the plant hormone auxin largely depends on the polar subcellular distribution of the plasma membrane PIN-FORMED (PIN) auxin transporters. PIN polarity switches in response to different developmental and environmental signals have been shown to redirect auxin fluxes mediating certain developmental responses. PIN phosphorylation at different sites and by different kinases is crucial for PIN function. Here we investigate the role of PIN phosphorylation during gravitropic response. Loss- and gain-of-function mutants in PINOID and related kinases but not in D6PK kinase as well as mutations mimicking constitutive dephosphorylated or phosphorylated status of two clusters of predicted phosphorylation sites partially disrupted PIN3 phosphorylation and caused defects in gravitropic bending in roots and hypocotyls. In particular, they impacted PIN3 polarity rearrangements in response to gravity and during feed-back regulation by auxin itself. Thus PIN phosphorylation, besides regulating transport activity and apical-basal targeting, is also important for the rapid polarity switches in response to environmental and endogenous signals.","lang":"eng"}],"author":[{"full_name":"Grones, Peter","last_name":"Grones","first_name":"Peter","id":"399876EC-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Melinda F","full_name":"Abas, Melinda F","last_name":"Abas","id":"3CFB3B1C-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0003-2140-7195","full_name":"Hajny, Jakub","last_name":"Hajny","first_name":"Jakub","id":"4800CC20-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Angharad","full_name":"Jones, Angharad","last_name":"Jones"},{"full_name":"Waidmann, Sascha","last_name":"Waidmann","first_name":"Sascha"},{"last_name":"Kleine Vehn","full_name":"Kleine Vehn, Jürgen","first_name":"Jürgen"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jirí","orcid":"0000-0002-8302-7596","last_name":"Friml","full_name":"Friml, Jirí"}]},{"language":[{"iso":"eng"}],"publisher":"Elsevier","scopus_import":"1","date_published":"2018-01-01T00:00:00Z","month":"01","date_created":"2018-12-11T11:44:20Z","department":[{"_id":"EvBe"}],"status":"public","intvolume":"        87","type":"journal_article","day":"01","page":"115 - 138","publication":"Advances in Botanical Research","title":"Transporters and mechanisms of hormone transport in arabidopsis","external_id":{"isi":["000453657800006"]},"doi":"10.1016/bs.abr.2018.09.007","year":"2018","related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"10303"}]},"isi":1,"author":[{"id":"4827E134-F248-11E8-B48F-1D18A9856A87","last_name":"Abualia","full_name":"Abualia, Rashed","orcid":"0000-0002-9357-9415","first_name":"Rashed"},{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","last_name":"Benková","full_name":"Benková, Eva","orcid":"0000-0002-8510-9739","first_name":"Eva"},{"first_name":"Benoît","last_name":"Lacombe","full_name":"Lacombe, Benoît"}],"abstract":[{"text":"Plant hormones as signalling molecules play an essential role in the control of plant growth and development. Typically, sites of hormonal action are usually distant from the site of biosynthesis thus relying on efficient transport mechanisms. Over the last decades, molecular identification of proteins and protein complexes involved in hormonal transport has started. Advanced screens for genes involved in hormonal transport in combination with transport assays using heterologous systems such as yeast, insect, or tobacco BY2 cells or Xenopus oocytes provided important insights into mechanisms underlying distribution of hormones in plant body and led to identification of principal transporters for each hormone. This review gives a short overview of the mechanisms of hormonal transport and transporters identified in Arabidopsis thaliana.","lang":"eng"}],"publication_status":"published","citation":{"ama":"Abualia R, Benková E, Lacombe B. Transporters and mechanisms of hormone transport in arabidopsis. <i>Advances in Botanical Research</i>. 2018;87:115-138. doi:<a href=\"https://doi.org/10.1016/bs.abr.2018.09.007\">10.1016/bs.abr.2018.09.007</a>","mla":"Abualia, Rashed, et al. “Transporters and Mechanisms of Hormone Transport in Arabidopsis.” <i>Advances in Botanical Research</i>, vol. 87, Elsevier, 2018, pp. 115–38, doi:<a href=\"https://doi.org/10.1016/bs.abr.2018.09.007\">10.1016/bs.abr.2018.09.007</a>.","ista":"Abualia R, Benková E, Lacombe B. 2018. Transporters and mechanisms of hormone transport in arabidopsis. Advances in Botanical Research. 87, 115–138.","short":"R. Abualia, E. Benková, B. Lacombe, Advances in Botanical Research 87 (2018) 115–138.","ieee":"R. Abualia, E. Benková, and B. Lacombe, “Transporters and mechanisms of hormone transport in arabidopsis,” <i>Advances in Botanical Research</i>, vol. 87. Elsevier, pp. 115–138, 2018.","apa":"Abualia, R., Benková, E., &#38; Lacombe, B. (2018). Transporters and mechanisms of hormone transport in arabidopsis. <i>Advances in Botanical Research</i>. Elsevier. <a href=\"https://doi.org/10.1016/bs.abr.2018.09.007\">https://doi.org/10.1016/bs.abr.2018.09.007</a>","chicago":"Abualia, Rashed, Eva Benková, and Benoît Lacombe. “Transporters and Mechanisms of Hormone Transport in Arabidopsis.” <i>Advances in Botanical Research</i>. Elsevier, 2018. <a href=\"https://doi.org/10.1016/bs.abr.2018.09.007\">https://doi.org/10.1016/bs.abr.2018.09.007</a>."},"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","oa_version":"None","quality_controlled":"1","_id":"47","date_updated":"2024-03-25T23:30:22Z","publist_id":"8007","volume":87,"article_processing_charge":"No"},{"type":"dissertation","day":"01","supervisor":[{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","first_name":"Eva","last_name":"Benková","full_name":"Benková, Eva","orcid":"0000-0002-8510-9739"}],"status":"public","page":"147","file_date_updated":"2020-12-02T23:30:08Z","date_published":"2018-01-01T00:00:00Z","month":"01","language":[{"iso":"eng"}],"publisher":"Institute of Science and Technology Austria","department":[{"_id":"EvBe"}],"degree_awarded":"PhD","has_accepted_license":"1","file":[{"date_created":"2019-04-05T09:37:56Z","checksum":"0c9d6d1c80d9857e6e545213467bbcb2","file_name":"2018_Hurny_thesis_source.docx","file_size":28112114,"date_updated":"2020-12-02T23:30:08Z","access_level":"closed","embargo_to":"open_access","file_id":"6226","creator":"dernst","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","relation":"source_file"},{"creator":"dernst","file_id":"6227","content_type":"application/pdf","relation":"main_file","date_created":"2019-04-05T09:37:55Z","checksum":"ecbe481a1413d270bd501b872c7ed54f","file_name":"2018_Hurny_thesis.pdf","file_size":12524427,"embargo":"2019-07-10","date_updated":"2020-12-02T09:52:16Z","access_level":"open_access"}],"date_created":"2018-12-11T11:47:03Z","citation":{"ama":"Hurny A. Identification and characterization of novel auxin-cytokinin cross-talk components. 2018. doi:<a href=\"https://doi.org/10.15479/AT:ISTA:th_930\">10.15479/AT:ISTA:th_930</a>","mla":"Hurny, Andrej. <i>Identification and Characterization of Novel Auxin-Cytokinin Cross-Talk Components</i>. Institute of Science and Technology Austria, 2018, doi:<a href=\"https://doi.org/10.15479/AT:ISTA:th_930\">10.15479/AT:ISTA:th_930</a>.","ista":"Hurny A. 2018. Identification and characterization of novel auxin-cytokinin cross-talk components. Institute of Science and Technology Austria.","short":"A. Hurny, Identification and Characterization of Novel Auxin-Cytokinin Cross-Talk Components, Institute of Science and Technology Austria, 2018.","ieee":"A. Hurny, “Identification and characterization of novel auxin-cytokinin cross-talk components,” Institute of Science and Technology Austria, 2018.","apa":"Hurny, A. (2018). <i>Identification and characterization of novel auxin-cytokinin cross-talk components</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT:ISTA:th_930\">https://doi.org/10.15479/AT:ISTA:th_930</a>","chicago":"Hurny, Andrej. “Identification and Characterization of Novel Auxin-Cytokinin Cross-Talk Components.” Institute of Science and Technology Austria, 2018. <a href=\"https://doi.org/10.15479/AT:ISTA:th_930\">https://doi.org/10.15479/AT:ISTA:th_930</a>."},"publication_status":"published","author":[{"id":"4DC4AF46-F248-11E8-B48F-1D18A9856A87","first_name":"Andrej","orcid":"0000-0003-3638-1426","full_name":"Hurny, Andrej","last_name":"Hurny"}],"abstract":[{"text":"The whole life cycle of plants as well as their responses to environmental stimuli is governed by a complex network of hormonal regulations. A number of studies have demonstrated an essential role of both auxin and cytokinin in the regulation of many aspects of plant growth and development including embryogenesis, postembryonic organogenic processes such as root, and shoot branching, root and shoot apical meristem activity and phyllotaxis. Over the last decades essential knowledge on the key molecular factors and pathways that spatio-temporally define auxin and cytokinin activities in the plant body has accumulated. However, how both hormonal pathways are interconnected by a complex network of interactions and feedback circuits that determines the final outcome of the individual hormone actions is still largely unknown. Root system architecture establishment and in particular formation of lateral organs is prime example of developmental process at whose regulation both auxin and cytokinin pathways converge. To dissect convergence points and pathways that tightly balance auxin - cytokinin antagonistic activities that determine the root branching pattern transcriptome profiling was applied. Genome wide expression analyses of the xylem pole pericycle, a tissue giving rise to lateral roots, led to identification of genes that are highly responsive to combinatorial auxin and cytokinin treatments and play an essential function in the auxin-cytokinin regulated root branching. SYNERGISTIC AUXIN CYTOKININ 1 (SYAC1) gene, which encodes for a protein of unknown function, was detected among the top candidate genes of which expression was synergistically up-regulated by simultaneous hormonal treatment. Plants with modulated SYAC1 activity exhibit severe defects in the root system establishment and attenuate developmental responses to both auxin and cytokinin. To explore the biological function of the SYAC1, we employed different strategies including expression pattern analysis, subcellular localization and phenotypic analyses of the syac1 loss-of-function and gain-of-function transgenic lines along with the identification of the SYAC1 interaction partners. Detailed functional characterization revealed that SYAC1 acts as a developmentally specific regulator of the secretory pathway to control deposition of cell wall components and thereby rapidly fine tune elongation growth.","lang":"eng"}],"article_processing_charge":"No","date_updated":"2023-09-07T12:41:06Z","publist_id":"7277","oa":1,"oa_version":"Published Version","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","publication_identifier":{"issn":["2663-337X"]},"_id":"539","year":"2018","doi":"10.15479/AT:ISTA:th_930","title":"Identification and characterization of novel auxin-cytokinin cross-talk components","pubrep_id":"930","tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"alternative_title":["ISTA Thesis"],"ddc":["570"],"related_material":{"record":[{"status":"public","relation":"part_of_dissertation","id":"1024"}]}},{"citation":{"ista":"Cavallari N, Nibau C, Fuchs A, Dadarou D, Barta A, Doonan J. 2018. The cyclin‐dependent kinase G group defines a thermo‐sensitive alternative splicing circuit modulating the expression of Arabidopsis ATU 2AF 65A. The Plant Journal. 94(6), 1010–1022.","short":"N. Cavallari, C. Nibau, A. Fuchs, D. Dadarou, A. Barta, J. Doonan, The Plant Journal 94 (2018) 1010–1022.","mla":"Cavallari, Nicola, et al. “The Cyclin‐dependent Kinase G Group Defines a Thermo‐sensitive Alternative Splicing Circuit Modulating the Expression of Arabidopsis ATU 2AF 65A.” <i>The Plant Journal</i>, vol. 94, no. 6, Wiley, 2018, pp. 1010–22, doi:<a href=\"https://doi.org/10.1111/tpj.13914\">10.1111/tpj.13914</a>.","ama":"Cavallari N, Nibau C, Fuchs A, Dadarou D, Barta A, Doonan J. The cyclin‐dependent kinase G group defines a thermo‐sensitive alternative splicing circuit modulating the expression of Arabidopsis ATU 2AF 65A. <i>The Plant Journal</i>. 2018;94(6):1010-1022. doi:<a href=\"https://doi.org/10.1111/tpj.13914\">10.1111/tpj.13914</a>","chicago":"Cavallari, Nicola, Candida Nibau, Armin Fuchs, Despoina Dadarou, Andrea Barta, and John Doonan. “The Cyclin‐dependent Kinase G Group Defines a Thermo‐sensitive Alternative Splicing Circuit Modulating the Expression of Arabidopsis ATU 2AF 65A.” <i>The Plant Journal</i>. Wiley, 2018. <a href=\"https://doi.org/10.1111/tpj.13914\">https://doi.org/10.1111/tpj.13914</a>.","ieee":"N. Cavallari, C. Nibau, A. Fuchs, D. Dadarou, A. Barta, and J. Doonan, “The cyclin‐dependent kinase G group defines a thermo‐sensitive alternative splicing circuit modulating the expression of Arabidopsis ATU 2AF 65A,” <i>The Plant Journal</i>, vol. 94, no. 6. Wiley, pp. 1010–1022, 2018.","apa":"Cavallari, N., Nibau, C., Fuchs, A., Dadarou, D., Barta, A., &#38; Doonan, J. (2018). The cyclin‐dependent kinase G group defines a thermo‐sensitive alternative splicing circuit modulating the expression of Arabidopsis ATU 2AF 65A. <i>The Plant Journal</i>. Wiley. <a href=\"https://doi.org/10.1111/tpj.13914\">https://doi.org/10.1111/tpj.13914</a>"},"publication_status":"published","author":[{"id":"457160E6-F248-11E8-B48F-1D18A9856A87","full_name":"Cavallari, Nicola","last_name":"Cavallari","first_name":"Nicola"},{"last_name":"Nibau","full_name":"Nibau, Candida","first_name":"Candida"},{"first_name":"Armin","last_name":"Fuchs","full_name":"Fuchs, Armin"},{"first_name":"Despoina","full_name":"Dadarou, Despoina","last_name":"Dadarou"},{"last_name":"Barta","full_name":"Barta, Andrea","first_name":"Andrea"},{"last_name":"Doonan","full_name":"Doonan, John","first_name":"John"}],"abstract":[{"lang":"eng","text":"The ability to adapt growth and development to temperature variations is crucial to generate plant varieties resilient to predicted temperature changes. However, the mechanisms underlying plant response to progressive increases in temperature have just started to be elucidated. Here, we report that the Cyclin-dependent Kinase G1 (CDKG1) is a central element in a thermo-sensitive mRNA splicing cascade that transduces changes in ambient temperature into differential expression of the fundamental spliceosome component, ATU2AF65A. CDKG1 is alternatively spliced in a temperature-dependent manner. We found that this process is partly dependent on both the Cyclin-dependent Kinase G2 (CDKG2) and the interacting co-factor CYCLIN L1 resulting in two distinct messenger RNAs. Relative abundance of both CDKG1 transcripts correlates with ambient temperature and possibly with different expression levels of the associated protein isoforms. Both CDKG1 alternative transcripts are necessary to fully complement the expression of ATU2AF65A across the temperature range. Our data support a previously unidentified temperature-dependent mechanism based on the alternative splicing of CDKG1 and regulated by CDKG2 and CYCLIN L1. We propose that changes in ambient temperature affect the relative abundance of CDKG1 transcripts and this in turn translates into differential CDKG1 protein expression coordinating the alternative splicing of ATU2AF65A. This article is protected by copyright. All rights reserved."}],"article_processing_charge":"No","volume":94,"oa":1,"publist_id":"7426","date_updated":"2023-09-19T10:07:08Z","quality_controlled":"1","oa_version":"Published Version","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","acknowledgement":"CN, DD and JHD were funded by the BBSRC (grant number BB/M009459/1). NC was funded by the VIPS Program of the Austrian Federal Ministry of Science and Research and the City of Vienna. AB and AF were supported by the Austrian Science Fund (FWF) [DK W1207; SFB RNAreg F43-P10]","_id":"403","doi":"10.1111/tpj.13914","year":"2018","external_id":{"isi":["000434365500008"]},"title":"The cyclin‐dependent kinase G group defines a thermo‐sensitive alternative splicing circuit modulating the expression of Arabidopsis ATU 2AF 65A","isi":1,"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"ddc":["580"],"type":"journal_article","day":"01","status":"public","intvolume":"        94","page":"1010 - 1022","file_date_updated":"2020-07-14T12:46:22Z","publication":"The Plant Journal","issue":"6","date_published":"2018-06-01T00:00:00Z","month":"06","language":[{"iso":"eng"}],"scopus_import":"1","publisher":"Wiley","department":[{"_id":"EvBe"}],"has_accepted_license":"1","file":[{"file_id":"5934","creator":"dernst","relation":"main_file","content_type":"application/pdf","checksum":"d9d3ad3215ac0e581731443fca312266","date_created":"2019-02-06T11:40:54Z","file_size":1543354,"file_name":"2018_PlantJourn_Cavallari.pdf","access_level":"open_access","date_updated":"2020-07-14T12:46:22Z"}],"date_created":"2018-12-11T11:46:17Z"},{"year":"2018","doi":"10.1016/j.phytochem.2018.02.015","title":"Design, synthesis and perception of fluorescently labeled isoprenoid cytokinins","external_id":{"isi":["000435623400001"]},"isi":1,"citation":{"chicago":"Kubiasová, Karolina, Václav Mik, Jaroslav Nisler, Martin Hönig, Alexandra Husičková, Lukáš Spíchal, Zuzana Pěkná, et al. “Design, Synthesis and Perception of Fluorescently Labeled Isoprenoid Cytokinins.” <i>Phytochemistry</i>. Elsevier, 2018. <a href=\"https://doi.org/10.1016/j.phytochem.2018.02.015\">https://doi.org/10.1016/j.phytochem.2018.02.015</a>.","apa":"Kubiasová, K., Mik, V., Nisler, J., Hönig, M., Husičková, A., Spíchal, L., … Plíhalová, L. (2018). Design, synthesis and perception of fluorescently labeled isoprenoid cytokinins. <i>Phytochemistry</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.phytochem.2018.02.015\">https://doi.org/10.1016/j.phytochem.2018.02.015</a>","ieee":"K. Kubiasová <i>et al.</i>, “Design, synthesis and perception of fluorescently labeled isoprenoid cytokinins,” <i>Phytochemistry</i>, vol. 150. Elsevier, pp. 1–11, 2018.","ista":"Kubiasová K, Mik V, Nisler J, Hönig M, Husičková A, Spíchal L, Pěkná Z, Šamajová O, Doležal K, Plíhal O, Benková E, Strnad M, Plíhalová L. 2018. Design, synthesis and perception of fluorescently labeled isoprenoid cytokinins. Phytochemistry. 150, 1–11.","short":"K. Kubiasová, V. Mik, J. Nisler, M. Hönig, A. Husičková, L. Spíchal, Z. Pěkná, O. Šamajová, K. Doležal, O. Plíhal, E. Benková, M. Strnad, L. Plíhalová, Phytochemistry 150 (2018) 1–11.","ama":"Kubiasová K, Mik V, Nisler J, et al. Design, synthesis and perception of fluorescently labeled isoprenoid cytokinins. <i>Phytochemistry</i>. 2018;150:1-11. doi:<a href=\"https://doi.org/10.1016/j.phytochem.2018.02.015\">10.1016/j.phytochem.2018.02.015</a>","mla":"Kubiasová, Karolina, et al. “Design, Synthesis and Perception of Fluorescently Labeled Isoprenoid Cytokinins.” <i>Phytochemistry</i>, vol. 150, Elsevier, 2018, pp. 1–11, doi:<a href=\"https://doi.org/10.1016/j.phytochem.2018.02.015\">10.1016/j.phytochem.2018.02.015</a>."},"publication_status":"published","author":[{"first_name":"Karolina","last_name":"Kubiasová","full_name":"Kubiasová, Karolina"},{"full_name":"Mik, Václav","last_name":"Mik","first_name":"Václav"},{"first_name":"Jaroslav","last_name":"Nisler","full_name":"Nisler, Jaroslav"},{"first_name":"Martin","last_name":"Hönig","full_name":"Hönig, Martin"},{"first_name":"Alexandra","last_name":"Husičková","full_name":"Husičková, Alexandra"},{"last_name":"Spíchal","full_name":"Spíchal, Lukáš","first_name":"Lukáš"},{"first_name":"Zuzana","last_name":"Pěkná","full_name":"Pěkná, Zuzana"},{"last_name":"Šamajová","full_name":"Šamajová, Olga","first_name":"Olga"},{"first_name":"Karel","last_name":"Doležal","full_name":"Doležal, Karel"},{"first_name":"Ondřej","full_name":"Plíhal, Ondřej","last_name":"Plíhal"},{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","first_name":"Eva","orcid":"0000-0002-8510-9739","full_name":"Benková, Eva","last_name":"Benková"},{"first_name":"Miroslav","last_name":"Strnad","full_name":"Strnad, Miroslav"},{"first_name":"Lucie","full_name":"Plíhalová, Lucie","last_name":"Plíhalová"}],"abstract":[{"text":"Isoprenoid cytokinins play a number of crucial roles in the regulation of plant growth and development. To study cytokinin receptor properties in plants, we designed and prepared fluorescent derivatives of 6-[(3-methylbut-2-en-1-yl)amino]purine (N6-isopentenyladenine, iP) with several fluorescent labels attached to the C2 or N9 atom of the purine moiety via a 2- or 6-carbon linker. The fluorescent labels included dansyl (DS), fluorescein (FC), 7-nitrobenzofurazan (NBD), rhodamine B (RhoB), coumarin (Cou), 7-(diethylamino)coumarin (DEAC) and cyanine 5 dye (Cy5). All prepared compounds were screened for affinity for the Arabidopsis thaliana cytokinin receptor (CRE1/AHK4). Although the attachment of the fluorescent labels to iP via the linkers mostly disrupted binding to the receptor, several fluorescent derivatives interacted well. For this reason, three derivatives, two rhodamine B and one 4-chloro-7-nitrobenzofurazan labeled iP were tested for their interaction with CRE1/AHK4 and Zea mays cytokinin receptors in detail. We further showed that the three derivatives were able to activate transcription of cytokinin response regulator ARR5 in Arabidopsis seedlings. The activity of fluorescently labeled cytokinins was compared with corresponding 6-dimethylaminopurine fluorescently labeled negative controls. Selected rhodamine B C2-labeled compounds 17, 18 and 4-chloro-7-nitrobenzofurazan N9-labeled compound 28 and their respective negative controls (19, 20 and 29, respectively) were used for in planta staining experiments in Arabidopsis thaliana cell suspension culture using live cell confocal microscopy.","lang":"eng"}],"article_processing_charge":"No","volume":150,"date_updated":"2023-09-11T12:53:11Z","publist_id":"7422","oa_version":"None","quality_controlled":"1","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","acknowledgement":"This work was supported by the Ministry of Education Youth and Sports, Czech Republic (grant LO1204 from the National Program of Sustainability I and Agricultural Research ) and by Czech Science Foundation grants 16-04184S , 501/10/1450 and 13-39982S and by IGA projects IGA_PrF_2018_033 and IGA_PrF_2018_023 . We would like to thank Jarmila Balonová, Olga Hustáková and Miroslava Šubová for their skillful technical assistance and Mgr. Tomáš Pospíšil, Ph.D. for his measurement of 1 H NMR and analysis of some 2D NMR spectral data. \r\n","_id":"407","date_published":"2018-06-01T00:00:00Z","month":"06","language":[{"iso":"eng"}],"scopus_import":"1","publisher":"Elsevier","department":[{"_id":"EvBe"}],"date_created":"2018-12-11T11:46:18Z","type":"journal_article","day":"01","status":"public","intvolume":"       150","page":"1-11","publication":"Phytochemistry"},{"ddc":["575"],"isi":1,"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"title":"Cup-shaped Cotyledon1 (CUC1) and CU2 regulate cytokinin homeostasis to determine ovule number in arabidopsis","external_id":{"isi":["000448163900015"]},"doi":"10.1093/jxb/ery281","year":"2018","acknowledgement":"This work was funded by the Ministry of Education, Youth and Sports of the Czech Republic through the National Program of Sustainability (grant no. LO1204).","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","oa_version":"Published Version","quality_controlled":"1","_id":"42","publist_id":"8012","volume":69,"oa":1,"date_updated":"2023-09-11T12:52:03Z","article_processing_charge":"No","author":[{"full_name":"Cucinotta, Mara","last_name":"Cucinotta","first_name":"Mara"},{"first_name":"Silvia","last_name":"Manrique","full_name":"Manrique, Silvia"},{"id":"33A3C818-F248-11E8-B48F-1D18A9856A87","first_name":"Candela","orcid":"0000-0003-1923-2410","full_name":"Cuesta, Candela","last_name":"Cuesta"},{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","first_name":"Eva","orcid":"0000-0002-8510-9739","full_name":"Benková, Eva","last_name":"Benková"},{"first_name":"Ondřej","full_name":"Novák, Ondřej","last_name":"Novák"},{"first_name":"Lucia","last_name":"Colombo","full_name":"Colombo, Lucia"}],"abstract":[{"text":"Seeds derive from ovules upon fertilization and therefore the total number of ovules determines the final seed yield, a fundamental trait in crop plants. Among the factors that co-ordinate the process of ovule formation, the transcription factors CUP-SHAPED COTYLEDON 1 (CUC1) and CUC2 and the hormone cytokinin (CK) have a particularly prominent role. Indeed, the absence of both CUC1 and CUC2 causes a severe reduction in ovule number, a phenotype that can be rescued by CK treatment. In this study, we combined CK quantification with an integrative genome-wide target identification approach to select Arabidopsis genes regulated by CUCs that are also involved in CK metabolism. We focused our attention on the functional characterization of UDP-GLUCOSYL TRANSFERASE 85A3 (UGT85A3) and UGT73C1, which are up-regulated in the absence of CUC1 and CUC2 and encode enzymes able to catalyse CK inactivation by O-glucosylation. Our results demonstrate a role for these UGTs as a link between CUCs and CK homeostasis, and highlight the importance of CUCs and CKs in the determination of seed yield.","lang":"eng"}],"publication_status":"published","citation":{"apa":"Cucinotta, M., Manrique, S., Cuesta, C., Benková, E., Novák, O., &#38; Colombo, L. (2018). Cup-shaped Cotyledon1 (CUC1) and CU2 regulate cytokinin homeostasis to determine ovule number in arabidopsis. <i>Journal of Experimental Botany</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/jxb/ery281\">https://doi.org/10.1093/jxb/ery281</a>","ieee":"M. Cucinotta, S. Manrique, C. Cuesta, E. Benková, O. Novák, and L. Colombo, “Cup-shaped Cotyledon1 (CUC1) and CU2 regulate cytokinin homeostasis to determine ovule number in arabidopsis,” <i>Journal of Experimental Botany</i>, vol. 69, no. 21. Oxford University Press, pp. 5169–5176, 2018.","chicago":"Cucinotta, Mara, Silvia Manrique, Candela Cuesta, Eva Benková, Ondřej Novák, and Lucia Colombo. “Cup-Shaped Cotyledon1 (CUC1) and CU2 Regulate Cytokinin Homeostasis to Determine Ovule Number in Arabidopsis.” <i>Journal of Experimental Botany</i>. Oxford University Press, 2018. <a href=\"https://doi.org/10.1093/jxb/ery281\">https://doi.org/10.1093/jxb/ery281</a>.","ama":"Cucinotta M, Manrique S, Cuesta C, Benková E, Novák O, Colombo L. Cup-shaped Cotyledon1 (CUC1) and CU2 regulate cytokinin homeostasis to determine ovule number in arabidopsis. <i>Journal of Experimental Botany</i>. 2018;69(21):5169-5176. doi:<a href=\"https://doi.org/10.1093/jxb/ery281\">10.1093/jxb/ery281</a>","mla":"Cucinotta, Mara, et al. “Cup-Shaped Cotyledon1 (CUC1) and CU2 Regulate Cytokinin Homeostasis to Determine Ovule Number in Arabidopsis.” <i>Journal of Experimental Botany</i>, vol. 69, no. 21, Oxford University Press, 2018, pp. 5169–76, doi:<a href=\"https://doi.org/10.1093/jxb/ery281\">10.1093/jxb/ery281</a>.","short":"M. Cucinotta, S. Manrique, C. Cuesta, E. Benková, O. Novák, L. Colombo, Journal of Experimental Botany 69 (2018) 5169–5176.","ista":"Cucinotta M, Manrique S, Cuesta C, Benková E, Novák O, Colombo L. 2018. Cup-shaped Cotyledon1 (CUC1) and CU2 regulate cytokinin homeostasis to determine ovule number in arabidopsis. Journal of Experimental Botany. 69(21), 5169–5176."},"date_created":"2018-12-11T11:44:19Z","file":[{"file_size":1292128,"file_name":"2018_JournalExperimBotany_Cucinotta.pdf","checksum":"ca3b6711040b1662488aeb3d1f961f13","date_created":"2018-12-17T10:44:16Z","access_level":"open_access","date_updated":"2020-07-14T12:46:25Z","relation":"main_file","content_type":"application/pdf","file_id":"5691","creator":"dernst"}],"department":[{"_id":"EvBe"}],"has_accepted_license":"1","language":[{"iso":"eng"}],"publisher":"Oxford University Press","scopus_import":"1","date_published":"2018-07-26T00:00:00Z","month":"07","file_date_updated":"2020-07-14T12:46:25Z","page":"5169 - 5176","issue":"21","publication":"Journal of Experimental Botany","status":"public","intvolume":"        69","type":"journal_article","day":"26"},{"author":[{"first_name":"Daniel","orcid":"0000-0002-6862-1247","full_name":"Von Wangenheim, Daniel","last_name":"Von Wangenheim","id":"49E91952-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Hauschild","full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522","first_name":"Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Fendrych, Matyas","last_name":"Fendrych","orcid":"0000-0002-9767-8699","first_name":"Matyas","id":"43905548-F248-11E8-B48F-1D18A9856A87"},{"id":"419EECCC-F248-11E8-B48F-1D18A9856A87","full_name":"Barone, Vanessa","last_name":"Barone","orcid":"0000-0003-2676-3367","first_name":"Vanessa"},{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","first_name":"Eva","orcid":"0000-0002-8510-9739","last_name":"Benková","full_name":"Benková, Eva"},{"first_name":"Jirí","last_name":"Friml","full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87"}],"abstract":[{"text":"Roots navigate through soil integrating environmental signals to orient their growth. The Arabidopsis root is a widely used model for developmental, physiological and cell biological studies. Live imaging greatly aids these efforts, but the horizontal sample position and continuous root tip displacement present significant difficulties. Here, we develop a confocal microscope setup for vertical sample mounting and integrated directional illumination. We present TipTracker – a custom software for automatic tracking of diverse moving objects usable on various microscope setups. Combined, this enables observation of root tips growing along the natural gravity vector over prolonged periods of time, as well as the ability to induce rapid gravity or light stimulation. We also track migrating cells in the developing zebrafish embryo, demonstrating the utility of this system in the acquisition of high-resolution data sets of dynamic samples. We provide detailed descriptions of the tools enabling the easy implementation on other microscopes.","lang":"eng"}],"citation":{"short":"D. von Wangenheim, R. Hauschild, M. Fendrych, V. Barone, E. Benková, J. Friml, ELife 6 (2017).","ista":"von Wangenheim D, Hauschild R, Fendrych M, Barone V, Benková E, Friml J. 2017. Live tracking of moving samples in confocal microscopy for vertically grown roots. eLife. 6, e26792.","mla":"von Wangenheim, Daniel, et al. “Live Tracking of Moving Samples in Confocal Microscopy for Vertically Grown Roots.” <i>ELife</i>, vol. 6, e26792, eLife Sciences Publications, 2017, doi:<a href=\"https://doi.org/10.7554/eLife.26792\">10.7554/eLife.26792</a>.","ama":"von Wangenheim D, Hauschild R, Fendrych M, Barone V, Benková E, Friml J. Live tracking of moving samples in confocal microscopy for vertically grown roots. <i>eLife</i>. 2017;6. doi:<a href=\"https://doi.org/10.7554/eLife.26792\">10.7554/eLife.26792</a>","chicago":"Wangenheim, Daniel von, Robert Hauschild, Matyas Fendrych, Vanessa Barone, Eva Benková, and Jiří Friml. “Live Tracking of Moving Samples in Confocal Microscopy for Vertically Grown Roots.” <i>ELife</i>. eLife Sciences Publications, 2017. <a href=\"https://doi.org/10.7554/eLife.26792\">https://doi.org/10.7554/eLife.26792</a>.","apa":"von Wangenheim, D., Hauschild, R., Fendrych, M., Barone, V., Benková, E., &#38; Friml, J. (2017). Live tracking of moving samples in confocal microscopy for vertically grown roots. <i>ELife</i>. eLife Sciences Publications. <a href=\"https://doi.org/10.7554/eLife.26792\">https://doi.org/10.7554/eLife.26792</a>","ieee":"D. von Wangenheim, R. Hauschild, M. Fendrych, V. Barone, E. Benková, and J. Friml, “Live tracking of moving samples in confocal microscopy for vertically grown roots,” <i>eLife</i>, vol. 6. eLife Sciences Publications, 2017."},"publication_status":"published","quality_controlled":"1","project":[{"grant_number":"291734","call_identifier":"FP7","_id":"25681D80-B435-11E9-9278-68D0E5697425","name":"International IST Postdoc Fellowship Programme"},{"_id":"2572ED28-B435-11E9-9278-68D0E5697425","name":"Molecular basis of root growth inhibition by auxin","call_identifier":"FWF","grant_number":"M02128"},{"grant_number":"I 1774-B16","_id":"2542D156-B435-11E9-9278-68D0E5697425","name":"Hormone cross-talk drives nutrient dependent plant development","call_identifier":"FWF"},{"call_identifier":"FP7","name":"Polarity and subcellular dynamics in plants","_id":"25716A02-B435-11E9-9278-68D0E5697425","grant_number":"282300"}],"oa_version":"Published Version","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","acknowledgement":"Funding: Marie Curie Actions (FP7/2007-2013 no 291734) to Daniel von Wangenheim; Austrian Science Fund (M 2128-B21) to Matyáš Fendrych; Austrian Science Fund (FWF01_I1774S) to Eva Benková; European Research Council (FP7/2007-2013 no 282300) to Jiří Friml. \r\nThe authors are grateful to the Miba Machine Shop at IST Austria for their contribution to the microscope setup and to Yvonne Kemper for reading, understanding and correcting the manuscript.\r\n#BioimagingFacility","_id":"946","article_processing_charge":"Yes","volume":6,"oa":1,"publist_id":"6471","date_updated":"2025-05-07T11:12:33Z","title":"Live tracking of moving samples in confocal microscopy for vertically grown roots","external_id":{"isi":["000404728300001"]},"pubrep_id":"847","ec_funded":1,"doi":"10.7554/eLife.26792","year":"2017","acknowledged_ssus":[{"_id":"M-Shop"},{"_id":"Bio"}],"ddc":["570"],"related_material":{"record":[{"id":"5566","relation":"popular_science","status":"public"}]},"isi":1,"article_number":"e26792","tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"status":"public","intvolume":"         6","type":"journal_article","day":"19","file_date_updated":"2020-07-14T12:48:15Z","publication":"eLife","language":[{"iso":"eng"}],"scopus_import":"1","publisher":"eLife Sciences Publications","date_published":"2017-06-19T00:00:00Z","month":"06","file":[{"content_type":"application/pdf","relation":"main_file","creator":"system","file_id":"5315","file_name":"IST-2017-847-v1+1_elife-26792-v2.pdf","file_size":19581847,"date_created":"2018-12-12T10:17:57Z","checksum":"9af3398cb0d81f99d79016a616df22e9","date_updated":"2020-07-14T12:48:15Z","access_level":"open_access"}],"date_created":"2018-12-11T11:49:21Z","department":[{"_id":"JiFr"},{"_id":"Bio"},{"_id":"CaHe"},{"_id":"EvBe"}],"has_accepted_license":"1"},{"doi":"10.1016/j.gde.2017.03.010","year":"2017","pubrep_id":"1017","title":"Spatiotemporal mechanisms of root branching","external_id":{"pmid":["28391060"],"isi":["000404880400013"]},"isi":1,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)"},"ddc":["575"],"publication_status":"published","citation":{"short":"K. Ötvös, E. Benková, Current Opinion in Genetics &#38; Development 45 (2017) 82–89.","ista":"Ötvös K, Benková E. 2017. Spatiotemporal mechanisms of root branching. Current Opinion in Genetics &#38; Development. 45, 82–89.","ama":"Ötvös K, Benková E. Spatiotemporal mechanisms of root branching. <i>Current Opinion in Genetics &#38; Development</i>. 2017;45:82-89. doi:<a href=\"https://doi.org/10.1016/j.gde.2017.03.010\">10.1016/j.gde.2017.03.010</a>","mla":"Ötvös, Krisztina, and Eva Benková. “Spatiotemporal Mechanisms of Root Branching.” <i>Current Opinion in Genetics &#38; Development</i>, vol. 45, Elsevier, 2017, pp. 82–89, doi:<a href=\"https://doi.org/10.1016/j.gde.2017.03.010\">10.1016/j.gde.2017.03.010</a>.","chicago":"Ötvös, Krisztina, and Eva Benková. “Spatiotemporal Mechanisms of Root Branching.” <i>Current Opinion in Genetics &#38; Development</i>. Elsevier, 2017. <a href=\"https://doi.org/10.1016/j.gde.2017.03.010\">https://doi.org/10.1016/j.gde.2017.03.010</a>.","apa":"Ötvös, K., &#38; Benková, E. (2017). Spatiotemporal mechanisms of root branching. <i>Current Opinion in Genetics &#38; Development</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.gde.2017.03.010\">https://doi.org/10.1016/j.gde.2017.03.010</a>","ieee":"K. Ötvös and E. Benková, “Spatiotemporal mechanisms of root branching,” <i>Current Opinion in Genetics &#38; Development</i>, vol. 45. Elsevier, pp. 82–89, 2017."},"author":[{"id":"29B901B0-F248-11E8-B48F-1D18A9856A87","first_name":"Krisztina","orcid":"0000-0002-5503-4983","full_name":"Ötvös, Krisztina","last_name":"Ötvös"},{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","first_name":"Eva","full_name":"Benková, Eva","last_name":"Benková","orcid":"0000-0002-8510-9739"}],"abstract":[{"text":"The fundamental tasks of the root system are, besides anchoring, mediating interactions between plant and soil and providing the plant with water and nutrients. The architecture of the root system is controlled by endogenous mechanisms that constantly integrate environmental signals, such as availability of nutrients and water. Extremely important for efficient soil exploitation and survival under less favorable conditions is the developmental flexibility of the root system that is largely determined by its postembryonic branching capacity. Modulation of initiation and outgrowth of lateral roots provides roots with an exceptional plasticity, allows optimal adjustment to underground heterogeneity, and enables effective soil exploitation and use of resources. Here we discuss recent advances in understanding the molecular mechanisms that shape the plant root system and integrate external cues to adapt to the changing environment.","lang":"eng"}],"volume":45,"publist_id":"6394","date_updated":"2023-09-22T09:48:15Z","oa":1,"article_processing_charge":"No","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","project":[{"grant_number":"I 1774-B16","_id":"2542D156-B435-11E9-9278-68D0E5697425","name":"Hormone cross-talk drives nutrient dependent plant development","call_identifier":"FWF"}],"quality_controlled":"1","oa_version":"Submitted Version","pmid":1,"_id":"1004","publication_identifier":{"issn":["0959437X"]},"date_published":"2017-08-01T00:00:00Z","month":"08","language":[{"iso":"eng"}],"publisher":"Elsevier","scopus_import":"1","department":[{"_id":"EvBe"}],"has_accepted_license":"1","file":[{"creator":"dernst","file_id":"6336","relation":"main_file","content_type":"application/pdf","success":1,"access_level":"open_access","date_updated":"2019-04-17T08:00:36Z","date_created":"2019-04-17T08:00:36Z","file_size":364133,"file_name":"Otvos_Benkova_CurOpDevBiol_2017.pdf"}],"date_created":"2018-12-11T11:49:38Z","type":"journal_article","day":"01","status":"public","intvolume":"        45","page":"82 - 89","file_date_updated":"2019-04-17T08:00:36Z","publication":"Current Opinion in Genetics & Development"}]