---
_id: '804'
abstract:
- lang: eng
text: Polysaccharides (carbohydrates) are key regulators of a large number of cell
biological processes. However, precise biochemical or genetic manipulation of
these often complex structures is laborious and hampers experimental structure–function
studies. Molecular Dynamics (MD) simulations provide a valuable alternative tool
to generate and test hypotheses on saccharide function. Yet, currently used MD
force fields often overestimate the aggregation propensity of polysaccharides,
affecting the usability of those simulations. Here we tested MARTINI, a popular
coarse-grained (CG) force field for biological macromolecules, for its ability
to accurately represent molecular forces between saccharides. To this end, we
calculated a thermodynamic solution property, the second virial coefficient of
the osmotic pressure (B22). Comparison with light scattering experiments revealed
a nonphysical aggregation of a prototypical polysaccharide in MARTINI, pointing
at an imbalance of the nonbonded solute–solute, solute–water, and water–water
interactions. This finding also applies to smaller oligosaccharides which were
all found to aggregate in simulations even at moderate concentrations, well below
their solubility limit. Finally, we explored the influence of the Lennard-Jones
(LJ) interaction between saccharide molecules and propose a simple scaling of
the LJ interaction strength that makes MARTINI more reliable for the simulation
of saccharides.
acknowledged_ssus:
- _id: ScienComp
acknowledgement: P.S.S. was supported by research fellowship 2811/1-1 from the German
Research Foundation (DFG), and M.S. was supported by EMBO Long Term Fellowship ALTF
187-2013 and Grant GC65-32 from the Interdisciplinary Centre for Mathematical and
Computational Modelling (ICM), University of Warsaw, Poland. The authors thank Antje
Potthast, Marek Cieplak, Tomasz Włodarski, and Damien Thompson for fruitful discussions
and the IST Austria Scientific Computing Facility for support.
article_processing_charge: No
author:
- first_name: Philipp S
full_name: Schmalhorst, Philipp S
id: 309D50DA-F248-11E8-B48F-1D18A9856A87
last_name: Schmalhorst
orcid: 0000-0002-5795-0133
- first_name: Felix
full_name: Deluweit, Felix
last_name: Deluweit
- first_name: Roger
full_name: Scherrers, Roger
last_name: Scherrers
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
- first_name: Mateusz K
full_name: Sikora, Mateusz K
id: 2F74BCDE-F248-11E8-B48F-1D18A9856A87
last_name: Sikora
citation:
ama: Schmalhorst PS, Deluweit F, Scherrers R, Heisenberg C-PJ, Sikora MK. Overcoming
the limitations of the MARTINI force field in simulations of polysaccharides.
Journal of Chemical Theory and Computation. 2017;13(10):5039-5053. doi:10.1021/acs.jctc.7b00374
apa: Schmalhorst, P. S., Deluweit, F., Scherrers, R., Heisenberg, C.-P. J., &
Sikora, M. K. (2017). Overcoming the limitations of the MARTINI force field in
simulations of polysaccharides. Journal of Chemical Theory and Computation.
American Chemical Society. https://doi.org/10.1021/acs.jctc.7b00374
chicago: Schmalhorst, Philipp S, Felix Deluweit, Roger Scherrers, Carl-Philipp J
Heisenberg, and Mateusz K Sikora. “Overcoming the Limitations of the MARTINI Force
Field in Simulations of Polysaccharides.” Journal of Chemical Theory and Computation.
American Chemical Society, 2017. https://doi.org/10.1021/acs.jctc.7b00374.
ieee: P. S. Schmalhorst, F. Deluweit, R. Scherrers, C.-P. J. Heisenberg, and M.
K. Sikora, “Overcoming the limitations of the MARTINI force field in simulations
of polysaccharides,” Journal of Chemical Theory and Computation, vol. 13,
no. 10. American Chemical Society, pp. 5039–5053, 2017.
ista: Schmalhorst PS, Deluweit F, Scherrers R, Heisenberg C-PJ, Sikora MK. 2017.
Overcoming the limitations of the MARTINI force field in simulations of polysaccharides.
Journal of Chemical Theory and Computation. 13(10), 5039–5053.
mla: Schmalhorst, Philipp S., et al. “Overcoming the Limitations of the MARTINI
Force Field in Simulations of Polysaccharides.” Journal of Chemical Theory
and Computation, vol. 13, no. 10, American Chemical Society, 2017, pp. 5039–53,
doi:10.1021/acs.jctc.7b00374.
short: P.S. Schmalhorst, F. Deluweit, R. Scherrers, C.-P.J. Heisenberg, M.K. Sikora,
Journal of Chemical Theory and Computation 13 (2017) 5039–5053.
date_created: 2018-12-11T11:48:35Z
date_published: 2017-10-10T00:00:00Z
date_updated: 2023-09-27T10:58:45Z
day: '10'
department:
- _id: CaHe
doi: 10.1021/acs.jctc.7b00374
external_id:
isi:
- '000412965700036'
intvolume: ' 13'
isi: 1
issue: '10'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://arxiv.org/abs/1704.03773
month: '10'
oa: 1
oa_version: Submitted Version
page: 5039 - 5053
publication: Journal of Chemical Theory and Computation
publication_identifier:
issn:
- '15499618'
publication_status: published
publisher: American Chemical Society
publist_id: '6847'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Overcoming the limitations of the MARTINI force field in simulations of polysaccharides
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 13
year: '2017'
...
---
_id: '676'
abstract:
- lang: eng
text: The segregation of different cell types into distinct tissues is a fundamental
process in metazoan development. Differences in cell adhesion and cortex tension
are commonly thought to drive cell sorting by regulating tissue surface tension
(TST). However, the role that differential TST plays in cell segregation within
the developing embryo is as yet unclear. Here, we have analyzed the role of differential
TST for germ layer progenitor cell segregation during zebrafish gastrulation.
Contrary to previous observations that differential TST drives germ layer progenitor
cell segregation in vitro, we show that germ layers display indistinguishable
TST within the gastrulating embryo, arguing against differential TST driving germ
layer progenitor cell segregation in vivo. We further show that the osmolarity
of the interstitial fluid (IF) is an important factor that influences germ layer
TST in vivo, and that lower osmolarity of the IF compared with standard cell culture
medium can explain why germ layers display differential TST in culture but not
in vivo. Finally, we show that directed migration of mesendoderm progenitors is
required for germ layer progenitor cell segregation and germ layer formation.
article_processing_charge: No
article_type: original
author:
- first_name: Gabriel
full_name: Krens, Gabriel
id: 2B819732-F248-11E8-B48F-1D18A9856A87
last_name: Krens
orcid: 0000-0003-4761-5996
- first_name: Jim
full_name: Veldhuis, Jim
last_name: Veldhuis
- first_name: Vanessa
full_name: Barone, Vanessa
id: 419EECCC-F248-11E8-B48F-1D18A9856A87
last_name: Barone
orcid: 0000-0003-2676-3367
- first_name: Daniel
full_name: Capek, Daniel
id: 31C42484-F248-11E8-B48F-1D18A9856A87
last_name: Capek
orcid: 0000-0001-5199-9940
- first_name: Jean-Léon
full_name: Maître, Jean-Léon
id: 48F1E0D8-F248-11E8-B48F-1D18A9856A87
last_name: Maître
orcid: 0000-0002-3688-1474
- first_name: Wayne
full_name: Brodland, Wayne
last_name: Brodland
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Krens G, Veldhuis J, Barone V, et al. Interstitial fluid osmolarity modulates
the action of differential tissue surface tension in progenitor cell segregation
during gastrulation. Development. 2017;144(10):1798-1806. doi:10.1242/dev.144964
apa: Krens, G., Veldhuis, J., Barone, V., Capek, D., Maître, J.-L., Brodland, W.,
& Heisenberg, C.-P. J. (2017). Interstitial fluid osmolarity modulates the
action of differential tissue surface tension in progenitor cell segregation during
gastrulation. Development. Company of Biologists. https://doi.org/10.1242/dev.144964
chicago: Krens, Gabriel, Jim Veldhuis, Vanessa Barone, Daniel Capek, Jean-Léon Maître,
Wayne Brodland, and Carl-Philipp J Heisenberg. “Interstitial Fluid Osmolarity
Modulates the Action of Differential Tissue Surface Tension in Progenitor Cell
Segregation during Gastrulation.” Development. Company of Biologists, 2017.
https://doi.org/10.1242/dev.144964.
ieee: G. Krens et al., “Interstitial fluid osmolarity modulates the action
of differential tissue surface tension in progenitor cell segregation during gastrulation,”
Development, vol. 144, no. 10. Company of Biologists, pp. 1798–1806, 2017.
ista: Krens G, Veldhuis J, Barone V, Capek D, Maître J-L, Brodland W, Heisenberg
C-PJ. 2017. Interstitial fluid osmolarity modulates the action of differential
tissue surface tension in progenitor cell segregation during gastrulation. Development.
144(10), 1798–1806.
mla: Krens, Gabriel, et al. “Interstitial Fluid Osmolarity Modulates the Action
of Differential Tissue Surface Tension in Progenitor Cell Segregation during Gastrulation.”
Development, vol. 144, no. 10, Company of Biologists, 2017, pp. 1798–806,
doi:10.1242/dev.144964.
short: G. Krens, J. Veldhuis, V. Barone, D. Capek, J.-L. Maître, W. Brodland, C.-P.J.
Heisenberg, Development 144 (2017) 1798–1806.
date_created: 2018-12-11T11:47:52Z
date_published: 2017-05-15T00:00:00Z
date_updated: 2024-03-25T23:30:13Z
day: '15'
ddc:
- '570'
department:
- _id: Bio
- _id: CaHe
doi: 10.1242/dev.144964
external_id:
pmid:
- '28512197'
file:
- access_level: open_access
checksum: bc25125fb664706cdf180e061429f91d
content_type: application/pdf
creator: dernst
date_created: 2019-09-24T06:56:22Z
date_updated: 2020-07-14T12:47:39Z
file_id: '6905'
file_name: 2017_Development_Krens.pdf
file_size: 8194516
relation: main_file
file_date_updated: 2020-07-14T12:47:39Z
has_accepted_license: '1'
intvolume: ' 144'
issue: '10'
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
page: 1798 - 1806
pmid: 1
publication: Development
publication_identifier:
issn:
- '09501991'
publication_status: published
publisher: Company of Biologists
publist_id: '7047'
quality_controlled: '1'
related_material:
record:
- id: '961'
relation: dissertation_contains
status: public
- id: '50'
relation: dissertation_contains
status: public
scopus_import: 1
status: public
title: Interstitial fluid osmolarity modulates the action of differential tissue surface
tension in progenitor cell segregation during gastrulation
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 144
year: '2017'
...
---
_id: '678'
abstract:
- lang: eng
text: The seminal observation that mechanical signals can elicit changes in biochemical
signalling within cells, a process commonly termed mechanosensation and mechanotransduction,
has revolutionized our understanding of the role of cell mechanics in various
fundamental biological processes, such as cell motility, adhesion, proliferation
and differentiation. In this Review, we will discuss how the interplay and feedback
between mechanical and biochemical signals control tissue morphogenesis and cell
fate specification in embryonic development.
author:
- first_name: Nicoletta
full_name: Petridou, Nicoletta
id: 2A003F6C-F248-11E8-B48F-1D18A9856A87
last_name: Petridou
orcid: 0000-0002-8451-1195
- first_name: Zoltan P
full_name: Spiro, Zoltan P
id: 426AD026-F248-11E8-B48F-1D18A9856A87
last_name: Spiro
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Petridou N, Spiro ZP, Heisenberg C-PJ. Multiscale force sensing in development.
Nature Cell Biology. 2017;19(6):581-588. doi:10.1038/ncb3524
apa: Petridou, N., Spiro, Z. P., & Heisenberg, C.-P. J. (2017). Multiscale force
sensing in development. Nature Cell Biology. Nature Publishing Group. https://doi.org/10.1038/ncb3524
chicago: Petridou, Nicoletta, Zoltan P Spiro, and Carl-Philipp J Heisenberg. “Multiscale
Force Sensing in Development.” Nature Cell Biology. Nature Publishing Group,
2017. https://doi.org/10.1038/ncb3524.
ieee: N. Petridou, Z. P. Spiro, and C.-P. J. Heisenberg, “Multiscale force sensing
in development,” Nature Cell Biology, vol. 19, no. 6. Nature Publishing
Group, pp. 581–588, 2017.
ista: Petridou N, Spiro ZP, Heisenberg C-PJ. 2017. Multiscale force sensing in development.
Nature Cell Biology. 19(6), 581–588.
mla: Petridou, Nicoletta, et al. “Multiscale Force Sensing in Development.” Nature
Cell Biology, vol. 19, no. 6, Nature Publishing Group, 2017, pp. 581–88, doi:10.1038/ncb3524.
short: N. Petridou, Z.P. Spiro, C.-P.J. Heisenberg, Nature Cell Biology 19 (2017)
581–588.
date_created: 2018-12-11T11:47:53Z
date_published: 2017-05-31T00:00:00Z
date_updated: 2021-01-12T08:08:59Z
day: '31'
department:
- _id: CaHe
doi: 10.1038/ncb3524
intvolume: ' 19'
issue: '6'
language:
- iso: eng
month: '05'
oa_version: None
page: 581 - 588
project:
- _id: 25236028-B435-11E9-9278-68D0E5697425
grant_number: ALTF534-2016
name: The generation and function of anisotropic tissue tension in zebrafish epiboly
(EMBO Fellowship)
publication: Nature Cell Biology
publication_identifier:
issn:
- '14657392'
publication_status: published
publisher: Nature Publishing Group
publist_id: '7040'
quality_controlled: '1'
scopus_import: 1
status: public
title: Multiscale force sensing in development
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 19
year: '2017'
...
---
_id: '686'
abstract:
- lang: eng
text: Tissues are thought to behave like fluids with a given surface tension. Differences
in tissue surface tension (TST) have been proposed to trigger cell sorting and
tissue envelopment. D'Arcy Thompson in his seminal book ‘On Growth and Form’ has
introduced this concept of differential TST as a key physical mechanism dictating
tissue formation and organization within the developing organism. Over the past
century, many studies have picked up the concept of differential TST and analyzed
the role and cell biological basis of TST in development, underlining the importance
and influence of this concept in developmental biology.
author:
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: 'Heisenberg C-PJ. D’Arcy Thompson’s ‘on growth and form’: From soap bubbles
to tissue self organization. Mechanisms of Development. 2017;145:32-37.
doi:10.1016/j.mod.2017.03.006'
apa: 'Heisenberg, C.-P. J. (2017). D’Arcy Thompson’s ‘on growth and form’: From
soap bubbles to tissue self organization. Mechanisms of Development. Elsevier.
https://doi.org/10.1016/j.mod.2017.03.006'
chicago: 'Heisenberg, Carl-Philipp J. “D’Arcy Thompson’s ‘on Growth and Form’: From
Soap Bubbles to Tissue Self Organization.” Mechanisms of Development. Elsevier,
2017. https://doi.org/10.1016/j.mod.2017.03.006.'
ieee: 'C.-P. J. Heisenberg, “D’Arcy Thompson’s ‘on growth and form’: From soap bubbles
to tissue self organization,” Mechanisms of Development, vol. 145. Elsevier,
pp. 32–37, 2017.'
ista: 'Heisenberg C-PJ. 2017. D’Arcy Thompson’s ‘on growth and form’: From soap
bubbles to tissue self organization. Mechanisms of Development. 145, 32–37.'
mla: 'Heisenberg, Carl-Philipp J. “D’Arcy Thompson’s ‘on Growth and Form’: From
Soap Bubbles to Tissue Self Organization.” Mechanisms of Development, vol.
145, Elsevier, 2017, pp. 32–37, doi:10.1016/j.mod.2017.03.006.'
short: C.-P.J. Heisenberg, Mechanisms of Development 145 (2017) 32–37.
date_created: 2018-12-11T11:47:55Z
date_published: 2017-06-01T00:00:00Z
date_updated: 2021-01-12T08:09:23Z
day: '01'
department:
- _id: CaHe
doi: 10.1016/j.mod.2017.03.006
intvolume: ' 145'
language:
- iso: eng
month: '06'
oa_version: None
page: 32 - 37
publication: Mechanisms of Development
publication_identifier:
issn:
- '09254773'
publication_status: published
publisher: Elsevier
publist_id: '7024'
quality_controlled: '1'
scopus_import: 1
status: public
title: 'D''Arcy Thompson''s ‘on growth and form’: From soap bubbles to tissue self
organization'
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 145
year: '2017'
...
---
_id: '728'
abstract:
- lang: eng
text: During animal development, cell-fate-specific changes in gene expression can
modify the material properties of a tissue and drive tissue morphogenesis. While
mechanistic insights into the genetic control of tissue-shaping events are beginning
to emerge, how tissue morphogenesis and mechanics can reciprocally impact cell-fate
specification remains relatively unexplored. Here we review recent findings reporting
how multicellular morphogenetic events and their underlying mechanical forces
can feed back into gene regulatory pathways to specify cell fate. We further discuss
emerging techniques that allow for the direct measurement and manipulation of
mechanical signals in vivo, offering unprecedented access to study mechanotransduction
during development. Examination of the mechanical control of cell fate during
tissue morphogenesis will pave the way to an integrated understanding of the design
principles that underlie robust tissue patterning in embryonic development.
article_processing_charge: No
author:
- first_name: Chii
full_name: Chan, Chii
last_name: Chan
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
- first_name: Takashi
full_name: Hiiragi, Takashi
last_name: Hiiragi
citation:
ama: Chan C, Heisenberg C-PJ, Hiiragi T. Coordination of morphogenesis and cell
fate specification in development. Current Biology. 2017;27(18):R1024-R1035.
doi:10.1016/j.cub.2017.07.010
apa: Chan, C., Heisenberg, C.-P. J., & Hiiragi, T. (2017). Coordination of morphogenesis
and cell fate specification in development. Current Biology. Cell Press.
https://doi.org/10.1016/j.cub.2017.07.010
chicago: Chan, Chii, Carl-Philipp J Heisenberg, and Takashi Hiiragi. “Coordination
of Morphogenesis and Cell Fate Specification in Development.” Current Biology.
Cell Press, 2017. https://doi.org/10.1016/j.cub.2017.07.010.
ieee: C. Chan, C.-P. J. Heisenberg, and T. Hiiragi, “Coordination of morphogenesis
and cell fate specification in development,” Current Biology, vol. 27,
no. 18. Cell Press, pp. R1024–R1035, 2017.
ista: Chan C, Heisenberg C-PJ, Hiiragi T. 2017. Coordination of morphogenesis and
cell fate specification in development. Current Biology. 27(18), R1024–R1035.
mla: Chan, Chii, et al. “Coordination of Morphogenesis and Cell Fate Specification
in Development.” Current Biology, vol. 27, no. 18, Cell Press, 2017, pp.
R1024–35, doi:10.1016/j.cub.2017.07.010.
short: C. Chan, C.-P.J. Heisenberg, T. Hiiragi, Current Biology 27 (2017) R1024–R1035.
date_created: 2018-12-11T11:48:11Z
date_published: 2017-09-18T00:00:00Z
date_updated: 2023-09-28T11:33:21Z
day: '18'
department:
- _id: CaHe
doi: 10.1016/j.cub.2017.07.010
external_id:
isi:
- '000411581800019'
intvolume: ' 27'
isi: 1
issue: '18'
language:
- iso: eng
month: '09'
oa_version: None
page: R1024 - R1035
publication: Current Biology
publication_identifier:
issn:
- '09609822'
publication_status: published
publisher: Cell Press
publist_id: '6949'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Coordination of morphogenesis and cell fate specification in development
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 27
year: '2017'
...
---
_id: '729'
abstract:
- lang: eng
text: The cellular mechanisms allowing tissues to efficiently regenerate are not
fully understood. In this issue of Developmental Cell, Cao et al. (2017)) discover
that during zebrafish heart regeneration, epicardial cells at the leading edge
of regenerating tissue undergo endoreplication, possibly due to increased tissue
tension, thereby boosting their regenerative capacity.
article_processing_charge: No
author:
- first_name: Zoltan P
full_name: Spiro, Zoltan P
id: 426AD026-F248-11E8-B48F-1D18A9856A87
last_name: Spiro
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Spiro ZP, Heisenberg C-PJ. Regeneration tensed up polyploidy takes the lead.
Developmental Cell. 2017;42(6):559-560. doi:10.1016/j.devcel.2017.09.008
apa: Spiro, Z. P., & Heisenberg, C.-P. J. (2017). Regeneration tensed up polyploidy
takes the lead. Developmental Cell. Cell Press. https://doi.org/10.1016/j.devcel.2017.09.008
chicago: Spiro, Zoltan P, and Carl-Philipp J Heisenberg. “Regeneration Tensed up
Polyploidy Takes the Lead.” Developmental Cell. Cell Press, 2017. https://doi.org/10.1016/j.devcel.2017.09.008.
ieee: Z. P. Spiro and C.-P. J. Heisenberg, “Regeneration tensed up polyploidy takes
the lead,” Developmental Cell, vol. 42, no. 6. Cell Press, pp. 559–560,
2017.
ista: Spiro ZP, Heisenberg C-PJ. 2017. Regeneration tensed up polyploidy takes the
lead. Developmental Cell. 42(6), 559–560.
mla: Spiro, Zoltan P., and Carl-Philipp J. Heisenberg. “Regeneration Tensed up Polyploidy
Takes the Lead.” Developmental Cell, vol. 42, no. 6, Cell Press, 2017,
pp. 559–60, doi:10.1016/j.devcel.2017.09.008.
short: Z.P. Spiro, C.-P.J. Heisenberg, Developmental Cell 42 (2017) 559–560.
date_created: 2018-12-11T11:48:11Z
date_published: 2017-01-01T00:00:00Z
date_updated: 2023-09-28T11:32:49Z
day: '01'
department:
- _id: CaHe
doi: 10.1016/j.devcel.2017.09.008
external_id:
isi:
- '000411582800003'
intvolume: ' 42'
isi: 1
issue: '6'
language:
- iso: eng
month: '01'
oa_version: None
page: 559 - 560
publication: Developmental Cell
publication_identifier:
issn:
- '15345807'
publication_status: published
publisher: Cell Press
publist_id: '6948'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Regeneration tensed up polyploidy takes the lead
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 42
year: '2017'
...
---
_id: '735'
abstract:
- lang: eng
text: Cell-cell contact formation constitutes an essential step in evolution, leading
to the differentiation of specialized cell types. However, remarkably little is
known about whether and how the interplay between contact formation and fate specification
affects development. Here, we identify a positive feedback loop between cell-cell
contact duration, morphogen signaling, and mesendoderm cell-fate specification
during zebrafish gastrulation. We show that long-lasting cell-cell contacts enhance
the competence of prechordal plate (ppl) progenitor cells to respond to Nodal
signaling, required for ppl cell-fate specification. We further show that Nodal
signaling promotes ppl cell-cell contact duration, generating a positive feedback
loop between ppl cell-cell contact duration and cell-fate specification. Finally,
by combining mathematical modeling and experimentation, we show that this feedback
determines whether anterior axial mesendoderm cells become ppl or, instead, turn
into endoderm. Thus, the interdependent activities of cell-cell signaling and
contact formation control fate diversification within the developing embryo.
article_processing_charge: No
author:
- first_name: Vanessa
full_name: Barone, Vanessa
id: 419EECCC-F248-11E8-B48F-1D18A9856A87
last_name: Barone
orcid: 0000-0003-2676-3367
- first_name: Moritz
full_name: Lang, Moritz
id: 29E0800A-F248-11E8-B48F-1D18A9856A87
last_name: Lang
- first_name: Gabriel
full_name: Krens, Gabriel
id: 2B819732-F248-11E8-B48F-1D18A9856A87
last_name: Krens
orcid: 0000-0003-4761-5996
- first_name: Saurabh
full_name: Pradhan, Saurabh
last_name: Pradhan
- first_name: Shayan
full_name: Shamipour, Shayan
id: 40B34FE2-F248-11E8-B48F-1D18A9856A87
last_name: Shamipour
- first_name: Keisuke
full_name: Sako, Keisuke
id: 3BED66BE-F248-11E8-B48F-1D18A9856A87
last_name: Sako
orcid: 0000-0002-6453-8075
- first_name: Mateusz K
full_name: Sikora, Mateusz K
id: 2F74BCDE-F248-11E8-B48F-1D18A9856A87
last_name: Sikora
- first_name: Calin C
full_name: Guet, Calin C
id: 47F8433E-F248-11E8-B48F-1D18A9856A87
last_name: Guet
orcid: 0000-0001-6220-2052
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Barone V, Lang M, Krens G, et al. An effective feedback loop between cell-cell
contact duration and morphogen signaling determines cell fate. Developmental
Cell. 2017;43(2):198-211. doi:10.1016/j.devcel.2017.09.014
apa: Barone, V., Lang, M., Krens, G., Pradhan, S., Shamipour, S., Sako, K., … Heisenberg,
C.-P. J. (2017). An effective feedback loop between cell-cell contact duration
and morphogen signaling determines cell fate. Developmental Cell. Cell
Press. https://doi.org/10.1016/j.devcel.2017.09.014
chicago: Barone, Vanessa, Moritz Lang, Gabriel Krens, Saurabh Pradhan, Shayan Shamipour,
Keisuke Sako, Mateusz K Sikora, Calin C Guet, and Carl-Philipp J Heisenberg. “An
Effective Feedback Loop between Cell-Cell Contact Duration and Morphogen Signaling
Determines Cell Fate.” Developmental Cell. Cell Press, 2017. https://doi.org/10.1016/j.devcel.2017.09.014.
ieee: V. Barone et al., “An effective feedback loop between cell-cell contact
duration and morphogen signaling determines cell fate,” Developmental Cell,
vol. 43, no. 2. Cell Press, pp. 198–211, 2017.
ista: Barone V, Lang M, Krens G, Pradhan S, Shamipour S, Sako K, Sikora MK, Guet
CC, Heisenberg C-PJ. 2017. An effective feedback loop between cell-cell contact
duration and morphogen signaling determines cell fate. Developmental Cell. 43(2),
198–211.
mla: Barone, Vanessa, et al. “An Effective Feedback Loop between Cell-Cell Contact
Duration and Morphogen Signaling Determines Cell Fate.” Developmental Cell,
vol. 43, no. 2, Cell Press, 2017, pp. 198–211, doi:10.1016/j.devcel.2017.09.014.
short: V. Barone, M. Lang, G. Krens, S. Pradhan, S. Shamipour, K. Sako, M.K. Sikora,
C.C. Guet, C.-P.J. Heisenberg, Developmental Cell 43 (2017) 198–211.
date_created: 2018-12-11T11:48:13Z
date_published: 2017-10-23T00:00:00Z
date_updated: 2024-03-25T23:30:21Z
day: '23'
department:
- _id: CaHe
- _id: CaGu
- _id: GaTk
doi: 10.1016/j.devcel.2017.09.014
ec_funded: 1
external_id:
isi:
- '000413443700011'
intvolume: ' 43'
isi: 1
issue: '2'
language:
- iso: eng
month: '10'
oa_version: None
page: 198 - 211
project:
- _id: 25681D80-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '291734'
name: International IST Postdoc Fellowship Programme
- _id: 252DD2A6-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I2058
name: 'Cell segregation in gastrulation: the role of cell fate specification'
publication: Developmental Cell
publication_identifier:
issn:
- '15345807'
publication_status: published
publisher: Cell Press
publist_id: '6934'
quality_controlled: '1'
related_material:
record:
- id: '961'
relation: dissertation_contains
status: public
- id: '8350'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: An effective feedback loop between cell-cell contact duration and morphogen
signaling determines cell fate
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 43
year: '2017'
...
---
_id: '661'
abstract:
- lang: eng
text: During embryonic development, mechanical forces are essential for cellular
rearrangements driving tissue morphogenesis. Here, we show that in the early zebrafish
embryo, friction forces are generated at the interface between anterior axial
mesoderm (prechordal plate, ppl) progenitors migrating towards the animal pole
and neurectoderm progenitors moving in the opposite direction towards the vegetal
pole of the embryo. These friction forces lead to global rearrangement of cells
within the neurectoderm and determine the position of the neural anlage. Using
a combination of experiments and simulations, we show that this process depends
on hydrodynamic coupling between neurectoderm and ppl as a result of E-cadherin-mediated
adhesion between those tissues. Our data thus establish the emergence of friction
forces at the interface between moving tissues as a critical force-generating
process shaping the embryo.
acknowledged_ssus:
- _id: SSU
author:
- first_name: Michael
full_name: Smutny, Michael
id: 3FE6E4E8-F248-11E8-B48F-1D18A9856A87
last_name: Smutny
orcid: 0000-0002-5920-9090
- first_name: Zsuzsa
full_name: Ákos, Zsuzsa
last_name: Ákos
- first_name: Silvia
full_name: Grigolon, Silvia
last_name: Grigolon
- first_name: Shayan
full_name: Shamipour, Shayan
id: 40B34FE2-F248-11E8-B48F-1D18A9856A87
last_name: Shamipour
- first_name: Verena
full_name: Ruprecht, Verena
last_name: Ruprecht
- first_name: Daniel
full_name: Capek, Daniel
id: 31C42484-F248-11E8-B48F-1D18A9856A87
last_name: Capek
orcid: 0000-0001-5199-9940
- first_name: Martin
full_name: Behrndt, Martin
id: 3ECECA3A-F248-11E8-B48F-1D18A9856A87
last_name: Behrndt
- first_name: Ekaterina
full_name: Papusheva, Ekaterina
id: 41DB591E-F248-11E8-B48F-1D18A9856A87
last_name: Papusheva
- first_name: Masazumi
full_name: Tada, Masazumi
last_name: Tada
- first_name: Björn
full_name: Hof, Björn
id: 3A374330-F248-11E8-B48F-1D18A9856A87
last_name: Hof
orcid: 0000-0003-2057-2754
- first_name: Tamás
full_name: Vicsek, Tamás
last_name: Vicsek
- first_name: Guillaume
full_name: Salbreux, Guillaume
last_name: Salbreux
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Smutny M, Ákos Z, Grigolon S, et al. Friction forces position the neural anlage.
Nature Cell Biology. 2017;19:306-317. doi:10.1038/ncb3492
apa: Smutny, M., Ákos, Z., Grigolon, S., Shamipour, S., Ruprecht, V., Capek, D.,
… Heisenberg, C.-P. J. (2017). Friction forces position the neural anlage. Nature
Cell Biology. Nature Publishing Group. https://doi.org/10.1038/ncb3492
chicago: Smutny, Michael, Zsuzsa Ákos, Silvia Grigolon, Shayan Shamipour, Verena
Ruprecht, Daniel Capek, Martin Behrndt, et al. “Friction Forces Position the Neural
Anlage.” Nature Cell Biology. Nature Publishing Group, 2017. https://doi.org/10.1038/ncb3492.
ieee: M. Smutny et al., “Friction forces position the neural anlage,” Nature
Cell Biology, vol. 19. Nature Publishing Group, pp. 306–317, 2017.
ista: Smutny M, Ákos Z, Grigolon S, Shamipour S, Ruprecht V, Capek D, Behrndt M,
Papusheva E, Tada M, Hof B, Vicsek T, Salbreux G, Heisenberg C-PJ. 2017. Friction
forces position the neural anlage. Nature Cell Biology. 19, 306–317.
mla: Smutny, Michael, et al. “Friction Forces Position the Neural Anlage.” Nature
Cell Biology, vol. 19, Nature Publishing Group, 2017, pp. 306–17, doi:10.1038/ncb3492.
short: M. Smutny, Z. Ákos, S. Grigolon, S. Shamipour, V. Ruprecht, D. Capek, M.
Behrndt, E. Papusheva, M. Tada, B. Hof, T. Vicsek, G. Salbreux, C.-P.J. Heisenberg,
Nature Cell Biology 19 (2017) 306–317.
date_created: 2018-12-11T11:47:46Z
date_published: 2017-03-27T00:00:00Z
date_updated: 2024-03-25T23:30:21Z
day: '27'
department:
- _id: CaHe
- _id: BjHo
- _id: Bio
doi: 10.1038/ncb3492
ec_funded: 1
external_id:
pmid:
- '28346437'
intvolume: ' 19'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://europepmc.org/articles/pmc5635970
month: '03'
oa: 1
oa_version: Submitted Version
page: 306 - 317
pmid: 1
project:
- _id: 25152F3A-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '306589'
name: Decoding the complexity of turbulence at its origin
- _id: 252ABD0A-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I 930-B20
name: Control of Epithelial Cell Layer Spreading in Zebrafish
publication: Nature Cell Biology
publication_identifier:
issn:
- '14657392'
publication_status: published
publisher: Nature Publishing Group
publist_id: '7074'
quality_controlled: '1'
related_material:
record:
- id: '50'
relation: dissertation_contains
status: public
- id: '8350'
relation: dissertation_contains
status: public
scopus_import: 1
status: public
title: Friction forces position the neural anlage
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 19
year: '2017'
...
---
_id: '946'
abstract:
- lang: eng
text: Roots navigate through soil integrating environmental signals to orient their
growth. The Arabidopsis root is a widely used model for developmental, physiological
and cell biological studies. Live imaging greatly aids these efforts, but the
horizontal sample position and continuous root tip displacement present significant
difficulties. Here, we develop a confocal microscope setup for vertical sample
mounting and integrated directional illumination. We present TipTracker – a custom
software for automatic tracking of diverse moving objects usable on various microscope
setups. Combined, this enables observation of root tips growing along the natural
gravity vector over prolonged periods of time, as well as the ability to induce
rapid gravity or light stimulation. We also track migrating cells in the developing
zebrafish embryo, demonstrating the utility of this system in the acquisition
of high-resolution data sets of dynamic samples. We provide detailed descriptions
of the tools enabling the easy implementation on other microscopes.
acknowledged_ssus:
- _id: M-Shop
- _id: Bio
acknowledgement: "Funding: Marie Curie Actions (FP7/2007-2013 no 291734) to Daniel
von Wangenheim; Austrian Science Fund (M 2128-B21) to Matyáš Fendrych; Austrian
Science Fund (FWF01_I1774S) to Eva Benková; European Research Council (FP7/2007-2013
no 282300) to Jiří Friml. \r\nThe authors are grateful to the Miba Machine Shop
at IST Austria for their contribution to the microscope setup and to Yvonne Kemper
for reading, understanding and correcting the manuscript.\r\n#BioimagingFacility"
article_number: e26792
article_processing_charge: Yes
author:
- first_name: Daniel
full_name: Von Wangenheim, Daniel
id: 49E91952-F248-11E8-B48F-1D18A9856A87
last_name: Von Wangenheim
orcid: 0000-0002-6862-1247
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Matyas
full_name: Fendrych, Matyas
id: 43905548-F248-11E8-B48F-1D18A9856A87
last_name: Fendrych
orcid: 0000-0002-9767-8699
- first_name: Vanessa
full_name: Barone, Vanessa
id: 419EECCC-F248-11E8-B48F-1D18A9856A87
last_name: Barone
orcid: 0000-0003-2676-3367
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: von Wangenheim D, Hauschild R, Fendrych M, Barone V, Benková E, Friml J. Live
tracking of moving samples in confocal microscopy for vertically grown roots.
eLife. 2017;6. doi:10.7554/eLife.26792
apa: von Wangenheim, D., Hauschild, R., Fendrych, M., Barone, V., Benková, E., &
Friml, J. (2017). Live tracking of moving samples in confocal microscopy for vertically
grown roots. ELife. eLife Sciences Publications. https://doi.org/10.7554/eLife.26792
chicago: Wangenheim, Daniel von, Robert Hauschild, Matyas Fendrych, Vanessa Barone,
Eva Benková, and Jiří Friml. “Live Tracking of Moving Samples in Confocal Microscopy
for Vertically Grown Roots.” ELife. eLife Sciences Publications, 2017.
https://doi.org/10.7554/eLife.26792.
ieee: D. von Wangenheim, R. Hauschild, M. Fendrych, V. Barone, E. Benková, and J.
Friml, “Live tracking of moving samples in confocal microscopy for vertically
grown roots,” eLife, vol. 6. eLife Sciences Publications, 2017.
ista: von Wangenheim D, Hauschild R, Fendrych M, Barone V, Benková E, Friml J. 2017.
Live tracking of moving samples in confocal microscopy for vertically grown roots.
eLife. 6, e26792.
mla: von Wangenheim, Daniel, et al. “Live Tracking of Moving Samples in Confocal
Microscopy for Vertically Grown Roots.” ELife, vol. 6, e26792, eLife Sciences
Publications, 2017, doi:10.7554/eLife.26792.
short: D. von Wangenheim, R. Hauschild, M. Fendrych, V. Barone, E. Benková, J. Friml,
ELife 6 (2017).
date_created: 2018-12-11T11:49:21Z
date_published: 2017-06-19T00:00:00Z
date_updated: 2025-05-07T11:12:33Z
day: '19'
ddc:
- '570'
department:
- _id: JiFr
- _id: Bio
- _id: CaHe
- _id: EvBe
doi: 10.7554/eLife.26792
ec_funded: 1
external_id:
isi:
- '000404728300001'
file:
- access_level: open_access
checksum: 9af3398cb0d81f99d79016a616df22e9
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:17:57Z
date_updated: 2020-07-14T12:48:15Z
file_id: '5315'
file_name: IST-2017-847-v1+1_elife-26792-v2.pdf
file_size: 19581847
relation: main_file
file_date_updated: 2020-07-14T12:48:15Z
has_accepted_license: '1'
intvolume: ' 6'
isi: 1
language:
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month: '06'
oa: 1
oa_version: Published Version
project:
- _id: 25681D80-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '291734'
name: International IST Postdoc Fellowship Programme
- _id: 2572ED28-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: M02128
name: Molecular basis of root growth inhibition by auxin
- _id: 2542D156-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I 1774-B16
name: Hormone cross-talk drives nutrient dependent plant development
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: eLife
publication_status: published
publisher: eLife Sciences Publications
publist_id: '6471'
pubrep_id: '847'
quality_controlled: '1'
related_material:
record:
- id: '5566'
relation: popular_science
status: public
scopus_import: '1'
status: public
title: Live tracking of moving samples in confocal microscopy for vertically grown
roots
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 6
year: '2017'
...
---
_id: '961'
abstract:
- lang: eng
text: Cell-cell contact formation constitutes the first step in the emergence of multicellularity in
evolution, thereby allowing the differentiation of specialized cell types. In metazoan
development, cell-cell contact formation is thought to influence cell fate specification,
and cell fate specification has been implicated in cell-cell contact
formation. However, remarkably little is yet known about whether and how the
interaction and feedback between cell-cell contact formation and cell fate specification
affect development. Here we identify a positive feedback loop between cell-cell contact duration, morphogen signaling and
mesendoderm cell fate specification during zebrafish gastrulation. We show that long
lasting cell-cell contacts enhance the competence of prechordal plate (ppl) progenitor
cells to respond to Nodal signaling, required for proper ppl cell fate specification. We further
show that Nodal signalling romotes ppl cell-cell contact duration, thereby generating an
effective positive feedback loop between ppl cell-cell contact duration and cell fate
specification. Finally, by using a combination of theoretical modeling and experimentation,
we show that this feedback loop determines whether anterior axial mesendoderm cells
become ppl progenitors or, instead, turn into endoderm progenitors. Our findings reveal
that the gene regulatory networks leading to cell fate diversification within the developing
embryo are controlled by the interdependent activities of cell-cell signaling and contact
formation.
acknowledgement: "Many people accompanied me during this trip: I would not have reached
my destination nor \r\nenjoyed the travelling without them. First of all, thanks
to CP. Thanks for making me part of \r\nyour team, always full of diverse, interesting
and incredibly competent people and thanks for \r\nall the good science I witnessed
\ and participated in. It has been a \r\nblast, an incredibly \r\nexciting
\ one! Thanks to JLo, for teaching me how to master my pipettes and
\ showing me \r\nthat science is a lot of fun. Many, many thanks to Gabby for teaching
me basically everything \r\nabout zebrafish and being always there to advice,
\ sugge\r\nst, support...and play fussball! \r\nThank you to Julien, for the
critical eye on things, Pedro, for all the invaluable feedback and \r\nthe amazing
kicker matches, and Keisuke, for showing me the light, and to the three of them
\r\ntogether for all the good laughs we\r\nhad. My start in Vienna would
\ have been a lot more \r\ndifficult without you guys. Also it would not
\ have been possible without Elena and Inês: \r\nthanks for helping setting
\ up this lab and for the dinners in Gugging. Thanks to Martin, for
\r\nhelping me understand \r\nthe physics behind biology. Thanks to Philipp,
\ for the interest and \r\nadvice, and to Michael, for the Viennise take on things.
Thanks to Julia, for putting up with \r\nbeing our technician and becoming a friend
in the process. And now to the newest members \r\nof th\r\ne lab. Thanks to Daniel
for the enthusiasm and the neverending energy and for all your \r\nhelp over the
years: thank you! To Jana, for showing me that one doesn’t give up, no matter \r\nwhat.
\ To Shayan, for being such a motivated student. To Matt, for helping
\ out\r\nwith coding \r\nand for finding punk solutions to data analysis problems.
Thanks to all the members of the \r\nlab, Verena, Hitoshi, Silvia, Conny, Karla,
Nicoletta, Zoltan, Peng, Benoit, Roland, Yuuta and \r\nFeyza, for the wonderful
\ atmosphere in the lab. Many than\r\nks to Koni and Deborah: doing \r\nexperiments
would have been much more difficult without your help. Special thanks to Katjia
\r\nfor setting up an amazing imaging facility and for building the best
\ team, Robert, Nasser, \r\nAnna and Doreen: thank you for putting up w\r\nith
all the late sortings and for helping with all \r\nthe technical problems. Thanks
to Eva, Verena and Matthias for keeping the fish happy. Big \r\nthanks to Harald
Janovjak for being a present and helpful committee member over the years \r\nand
\ to Patrick Lemaire f\r\nor the helpful insight and extremely interesting
\ discussion we had \r\nabout the project. Also, this journey would not
\ have been the same without all the friends \r\nthat I met in Dresden and
then in Vienna: Daniele, Claire, Kuba, Steffi, Harold, Dejan, Irene, \r\nFab\r\nienne,
Hande, Tiago, Marianne, Jon, Srdjan, Branca, Uli, Murat, Alex, Conny, Christoph,
\r\nCaro, Simone, Barbara, Felipe, Dama, Jose, Hubert and many others that filled
my days with \r\nfun and support. A special thank to my family, always close even
if they are \r\nkilometers away. \r\nGrazie ai miei fratelli, Nunzio e William,
\ e alla mia mamma, per essermi sempre vicini pur \r\nvivendo a chilometri
di distanza. And, last but not least, thanks to Moritz, for putting up with \r\nthe
crazy life of a scientist, the living apart for\r\nso long, never knowing when things
are going \r\nto happen. Thanks for being a great partner and my number one fan!"
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Vanessa
full_name: Barone, Vanessa
id: 419EECCC-F248-11E8-B48F-1D18A9856A87
last_name: Barone
orcid: 0000-0003-2676-3367
citation:
ama: 'Barone V. Cell adhesion and cell fate: An effective feedback loop during zebrafish
gastrulation. 2017. doi:10.15479/AT:ISTA:th_825'
apa: 'Barone, V. (2017). Cell adhesion and cell fate: An effective feedback loop
during zebrafish gastrulation. Institute of Science and Technology Austria.
https://doi.org/10.15479/AT:ISTA:th_825'
chicago: 'Barone, Vanessa. “Cell Adhesion and Cell Fate: An Effective Feedback Loop
during Zebrafish Gastrulation.” Institute of Science and Technology Austria, 2017.
https://doi.org/10.15479/AT:ISTA:th_825.'
ieee: 'V. Barone, “Cell adhesion and cell fate: An effective feedback loop during
zebrafish gastrulation,” Institute of Science and Technology Austria, 2017.'
ista: 'Barone V. 2017. Cell adhesion and cell fate: An effective feedback loop during
zebrafish gastrulation. Institute of Science and Technology Austria.'
mla: 'Barone, Vanessa. Cell Adhesion and Cell Fate: An Effective Feedback Loop
during Zebrafish Gastrulation. Institute of Science and Technology Austria,
2017, doi:10.15479/AT:ISTA:th_825.'
short: 'V. Barone, Cell Adhesion and Cell Fate: An Effective Feedback Loop during
Zebrafish Gastrulation, Institute of Science and Technology Austria, 2017.'
date_created: 2018-12-11T11:49:25Z
date_published: 2017-03-01T00:00:00Z
date_updated: 2023-09-27T14:16:45Z
day: '01'
ddc:
- '570'
- '590'
degree_awarded: PhD
department:
- _id: CaHe
doi: 10.15479/AT:ISTA:th_825
file:
- access_level: closed
checksum: 242f88c87f2cf267bf05049fa26a687b
content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document
creator: dernst
date_created: 2019-04-05T08:36:52Z
date_updated: 2020-07-14T12:48:16Z
file_id: '6205'
file_name: 2017_Barone_thesis_final.docx
file_size: 14497822
relation: source_file
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checksum: ba5b0613ed8bade73a409acdd880fb8a
content_type: application/pdf
creator: dernst
date_created: 2019-04-05T08:36:52Z
date_updated: 2020-07-14T12:48:16Z
file_id: '6206'
file_name: 2017_Barone_thesis_.pdf
file_size: 14995941
relation: main_file
file_date_updated: 2020-07-14T12:48:16Z
has_accepted_license: '1'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
page: '109'
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
publist_id: '6444'
pubrep_id: '825'
related_material:
record:
- id: '1100'
relation: part_of_dissertation
status: public
- id: '1537'
relation: part_of_dissertation
status: public
- id: '1912'
relation: part_of_dissertation
status: public
- id: '2926'
relation: part_of_dissertation
status: public
- id: '3246'
relation: part_of_dissertation
status: public
- id: '676'
relation: part_of_dissertation
status: public
- id: '735'
relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
title: 'Cell adhesion and cell fate: An effective feedback loop during zebrafish gastrulation'
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2017'
...
---
_id: '1025'
abstract:
- lang: eng
text: Many organ surfaces are covered by a protective epithelial-cell layer. It
emerges that such layers are maintained by cell stretching that triggers cell
division mediated by the force-sensitive ion-channel protein Piezo1. See Letter
p.118
article_processing_charge: No
author:
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: 'Heisenberg C-PJ. Cell biology: Stretched divisions. Nature. 2017;543(7643):43-44.
doi:10.1038/nature21502'
apa: 'Heisenberg, C.-P. J. (2017). Cell biology: Stretched divisions. Nature.
Nature Publishing Group. https://doi.org/10.1038/nature21502'
chicago: 'Heisenberg, Carl-Philipp J. “Cell Biology: Stretched Divisions.” Nature.
Nature Publishing Group, 2017. https://doi.org/10.1038/nature21502.'
ieee: 'C.-P. J. Heisenberg, “Cell biology: Stretched divisions,” Nature,
vol. 543, no. 7643. Nature Publishing Group, pp. 43–44, 2017.'
ista: 'Heisenberg C-PJ. 2017. Cell biology: Stretched divisions. Nature. 543(7643),
43–44.'
mla: 'Heisenberg, Carl-Philipp J. “Cell Biology: Stretched Divisions.” Nature,
vol. 543, no. 7643, Nature Publishing Group, 2017, pp. 43–44, doi:10.1038/nature21502.'
short: C.-P.J. Heisenberg, Nature 543 (2017) 43–44.
date_created: 2018-12-11T11:49:45Z
date_published: 2017-03-02T00:00:00Z
date_updated: 2023-09-22T09:26:59Z
day: '02'
department:
- _id: CaHe
doi: 10.1038/nature21502
external_id:
isi:
- '000395671500025'
intvolume: ' 543'
isi: 1
issue: '7643'
language:
- iso: eng
month: '03'
oa_version: None
page: 43 - 44
publication: Nature
publication_identifier:
issn:
- '00280836'
publication_status: published
publisher: Nature Publishing Group
publist_id: '6367'
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Cell biology: Stretched divisions'
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 543
year: '2017'
...
---
_id: '1096'
author:
- first_name: Cornelia
full_name: Schwayer, Cornelia
id: 3436488C-F248-11E8-B48F-1D18A9856A87
last_name: Schwayer
orcid: 0000-0001-5130-2226
- first_name: Mateusz K
full_name: Sikora, Mateusz K
id: 2F74BCDE-F248-11E8-B48F-1D18A9856A87
last_name: Sikora
- first_name: Jana
full_name: Slovakova, Jana
id: 30F3F2F0-F248-11E8-B48F-1D18A9856A87
last_name: Slovakova
- first_name: Roland
full_name: Kardos, Roland
id: 4039350E-F248-11E8-B48F-1D18A9856A87
last_name: Kardos
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Schwayer C, Sikora MK, Slovakova J, Kardos R, Heisenberg C-PJ. Actin rings
of power. Developmental Cell. 2016;37(6):493-506. doi:10.1016/j.devcel.2016.05.024
apa: Schwayer, C., Sikora, M. K., Slovakova, J., Kardos, R., & Heisenberg, C.-P.
J. (2016). Actin rings of power. Developmental Cell. Cell Press. https://doi.org/10.1016/j.devcel.2016.05.024
chicago: Schwayer, Cornelia, Mateusz K Sikora, Jana Slovakova, Roland Kardos, and
Carl-Philipp J Heisenberg. “Actin Rings of Power.” Developmental Cell.
Cell Press, 2016. https://doi.org/10.1016/j.devcel.2016.05.024.
ieee: C. Schwayer, M. K. Sikora, J. Slovakova, R. Kardos, and C.-P. J. Heisenberg,
“Actin rings of power,” Developmental Cell, vol. 37, no. 6. Cell Press,
pp. 493–506, 2016.
ista: Schwayer C, Sikora MK, Slovakova J, Kardos R, Heisenberg C-PJ. 2016. Actin
rings of power. Developmental Cell. 37(6), 493–506.
mla: Schwayer, Cornelia, et al. “Actin Rings of Power.” Developmental Cell,
vol. 37, no. 6, Cell Press, 2016, pp. 493–506, doi:10.1016/j.devcel.2016.05.024.
short: C. Schwayer, M.K. Sikora, J. Slovakova, R. Kardos, C.-P.J. Heisenberg, Developmental
Cell 37 (2016) 493–506.
date_created: 2018-12-11T11:50:07Z
date_published: 2016-06-20T00:00:00Z
date_updated: 2023-09-07T12:56:41Z
day: '20'
department:
- _id: CaHe
doi: 10.1016/j.devcel.2016.05.024
intvolume: ' 37'
issue: '6'
language:
- iso: eng
month: '06'
oa_version: None
page: 493 - 506
publication: Developmental Cell
publication_status: published
publisher: Cell Press
publist_id: '6279'
quality_controlled: '1'
related_material:
record:
- id: '7186'
relation: part_of_dissertation
status: public
scopus_import: 1
status: public
title: Actin rings of power
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 37
year: '2016'
...
---
_id: '1100'
abstract:
- lang: eng
text: During metazoan development, the temporal pattern of morphogen signaling is
critical for organizing cell fates in space and time. Yet, tools for temporally
controlling morphogen signaling within the embryo are still scarce. Here, we developed
a photoactivatable Nodal receptor to determine how the temporal pattern of Nodal
signaling affects cell fate specification during zebrafish gastrulation. By using
this receptor to manipulate the duration of Nodal signaling in vivo by light,
we show that extended Nodal signaling within the organizer promotes prechordal
plate specification and suppresses endoderm differentiation. Endoderm differentiation
is suppressed by extended Nodal signaling inducing expression of the transcriptional
repressor goosecoid (gsc) in prechordal plate progenitors, which in turn restrains
Nodal signaling from upregulating the endoderm differentiation gene sox17 within
these cells. Thus, optogenetic manipulation of Nodal signaling identifies a critical
role of Nodal signaling duration for organizer cell fate specification during
gastrulation.
acknowledged_ssus:
- _id: SSU
acknowledgement: 'We are grateful to members of the C.-P.H. and H.J. labs for discussions,
R. Hauschild and the different Scientific Service Units at IST Austria for technical
help, M. Dravecka for performing initial experiments, A. Schier for reading an earlier
version of the manuscript, K.W. Rogers for technical help, and C. Hill, A. Bruce,
and L. Solnica-Krezel for sending plasmids. This work was supported by grants from
the Austrian Science Foundation (FWF): (T560-B17) and (I 812-B12) to V.R. and C.-P.H.,
and from the European Union (EU FP7): (6275) to H.J. A.I.-P. is supported by a Ramon
Areces fellowship.'
author:
- first_name: Keisuke
full_name: Sako, Keisuke
id: 3BED66BE-F248-11E8-B48F-1D18A9856A87
last_name: Sako
orcid: 0000-0002-6453-8075
- first_name: Saurabh
full_name: Pradhan, Saurabh
last_name: Pradhan
- first_name: Vanessa
full_name: Barone, Vanessa
id: 419EECCC-F248-11E8-B48F-1D18A9856A87
last_name: Barone
orcid: 0000-0003-2676-3367
- first_name: Álvaro
full_name: Inglés Prieto, Álvaro
id: 2A9DB292-F248-11E8-B48F-1D18A9856A87
last_name: Inglés Prieto
orcid: 0000-0002-5409-8571
- first_name: Patrick
full_name: Mueller, Patrick
last_name: Mueller
- first_name: Verena
full_name: Ruprecht, Verena
id: 4D71A03A-F248-11E8-B48F-1D18A9856A87
last_name: Ruprecht
orcid: 0000-0003-4088-8633
- first_name: Daniel
full_name: Capek, Daniel
id: 31C42484-F248-11E8-B48F-1D18A9856A87
last_name: Capek
orcid: 0000-0001-5199-9940
- first_name: Sanjeev
full_name: Galande, Sanjeev
last_name: Galande
- first_name: Harald L
full_name: Janovjak, Harald L
id: 33BA6C30-F248-11E8-B48F-1D18A9856A87
last_name: Janovjak
orcid: 0000-0002-8023-9315
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Sako K, Pradhan S, Barone V, et al. Optogenetic control of nodal signaling
reveals a temporal pattern of nodal signaling regulating cell fate specification
during gastrulation. Cell Reports. 2016;16(3):866-877. doi:10.1016/j.celrep.2016.06.036
apa: Sako, K., Pradhan, S., Barone, V., Inglés Prieto, Á., Mueller, P., Ruprecht,
V., … Heisenberg, C.-P. J. (2016). Optogenetic control of nodal signaling reveals
a temporal pattern of nodal signaling regulating cell fate specification during
gastrulation. Cell Reports. Cell Press. https://doi.org/10.1016/j.celrep.2016.06.036
chicago: Sako, Keisuke, Saurabh Pradhan, Vanessa Barone, Álvaro Inglés Prieto, Patrick
Mueller, Verena Ruprecht, Daniel Capek, Sanjeev Galande, Harald L Janovjak, and
Carl-Philipp J Heisenberg. “Optogenetic Control of Nodal Signaling Reveals a Temporal
Pattern of Nodal Signaling Regulating Cell Fate Specification during Gastrulation.”
Cell Reports. Cell Press, 2016. https://doi.org/10.1016/j.celrep.2016.06.036.
ieee: K. Sako et al., “Optogenetic control of nodal signaling reveals a temporal
pattern of nodal signaling regulating cell fate specification during gastrulation,”
Cell Reports, vol. 16, no. 3. Cell Press, pp. 866–877, 2016.
ista: Sako K, Pradhan S, Barone V, Inglés Prieto Á, Mueller P, Ruprecht V, Capek
D, Galande S, Janovjak HL, Heisenberg C-PJ. 2016. Optogenetic control of nodal
signaling reveals a temporal pattern of nodal signaling regulating cell fate specification
during gastrulation. Cell Reports. 16(3), 866–877.
mla: Sako, Keisuke, et al. “Optogenetic Control of Nodal Signaling Reveals a Temporal
Pattern of Nodal Signaling Regulating Cell Fate Specification during Gastrulation.”
Cell Reports, vol. 16, no. 3, Cell Press, 2016, pp. 866–77, doi:10.1016/j.celrep.2016.06.036.
short: K. Sako, S. Pradhan, V. Barone, Á. Inglés Prieto, P. Mueller, V. Ruprecht,
D. Capek, S. Galande, H.L. Janovjak, C.-P.J. Heisenberg, Cell Reports 16 (2016)
866–877.
date_created: 2018-12-11T11:50:08Z
date_published: 2016-07-19T00:00:00Z
date_updated: 2024-03-25T23:30:13Z
day: '19'
ddc:
- '570'
- '576'
department:
- _id: CaHe
- _id: HaJa
doi: 10.1016/j.celrep.2016.06.036
ec_funded: 1
file:
- access_level: open_access
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:11:04Z
date_updated: 2018-12-12T10:11:04Z
file_id: '4857'
file_name: IST-2017-754-v1+1_1-s2.0-S2211124716307768-main.pdf
file_size: 3921947
relation: main_file
file_date_updated: 2018-12-12T10:11:04Z
has_accepted_license: '1'
intvolume: ' 16'
issue: '3'
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
page: 866 - 877
project:
- _id: 2529486C-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: T 560-B17
name: Cell- and Tissue Mechanics in Zebrafish Germ Layer Formation
- _id: 2527D5CC-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I 812-B12
name: Cell Cortex and Germ Layer Formation in Zebrafish Gastrulation
- _id: 25548C20-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '303564'
name: Microbial Ion Channels for Synthetic Neurobiology
publication: Cell Reports
publication_status: published
publisher: Cell Press
publist_id: '6275'
pubrep_id: '754'
quality_controlled: '1'
related_material:
record:
- id: '961'
relation: dissertation_contains
status: public
- id: '50'
relation: dissertation_contains
status: public
scopus_import: 1
status: public
title: Optogenetic control of nodal signaling reveals a temporal pattern of nodal
signaling regulating cell fate specification during gastrulation
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 16
year: '2016'
...
---
_id: '1239'
abstract:
- lang: eng
text: Nonadherent polarized cells have been observed to have a pearlike, elongated
shape. Using a minimal model that describes the cell cortex as a thin layer of
contractile active gel, we show that the anisotropy of active stresses, controlled
by cortical viscosity and filament ordering, can account for this morphology.
The predicted shapes can be determined from the flow pattern only; they prove
to be independent of the mechanism at the origin of the cortical flow, and are
only weakly sensitive to the cytoplasmic rheology. In the case of actin flows
resulting from a contractile instability, we propose a phase diagram of three-dimensional
cell shapes that encompasses nonpolarized spherical, elongated, as well as oblate
shapes, all of which have been observed in experiment.
acknowledgement: 'V. R. acknowledges support by the Austrian Science Fund (FWF): (Grant
No. T560-B17).'
article_number: '028102'
author:
- first_name: Andrew
full_name: Callan Jones, Andrew
last_name: Callan Jones
- first_name: Verena
full_name: Ruprecht, Verena
id: 4D71A03A-F248-11E8-B48F-1D18A9856A87
last_name: Ruprecht
orcid: 0000-0003-4088-8633
- first_name: Stefan
full_name: Wieser, Stefan
id: 355AA5A0-F248-11E8-B48F-1D18A9856A87
last_name: Wieser
orcid: 0000-0002-2670-2217
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
- first_name: Raphaël
full_name: Voituriez, Raphaël
last_name: Voituriez
citation:
ama: Callan Jones A, Ruprecht V, Wieser S, Heisenberg C-PJ, Voituriez R. Cortical
flow-driven shapes of nonadherent cells. Physical Review Letters. 2016;116(2).
doi:10.1103/PhysRevLett.116.028102
apa: Callan Jones, A., Ruprecht, V., Wieser, S., Heisenberg, C.-P. J., & Voituriez,
R. (2016). Cortical flow-driven shapes of nonadherent cells. Physical Review
Letters. American Physical Society. https://doi.org/10.1103/PhysRevLett.116.028102
chicago: Callan Jones, Andrew, Verena Ruprecht, Stefan Wieser, Carl-Philipp J Heisenberg,
and Raphaël Voituriez. “Cortical Flow-Driven Shapes of Nonadherent Cells.” Physical
Review Letters. American Physical Society, 2016. https://doi.org/10.1103/PhysRevLett.116.028102.
ieee: A. Callan Jones, V. Ruprecht, S. Wieser, C.-P. J. Heisenberg, and R. Voituriez,
“Cortical flow-driven shapes of nonadherent cells,” Physical Review Letters,
vol. 116, no. 2. American Physical Society, 2016.
ista: Callan Jones A, Ruprecht V, Wieser S, Heisenberg C-PJ, Voituriez R. 2016.
Cortical flow-driven shapes of nonadherent cells. Physical Review Letters. 116(2),
028102.
mla: Callan Jones, Andrew, et al. “Cortical Flow-Driven Shapes of Nonadherent Cells.”
Physical Review Letters, vol. 116, no. 2, 028102, American Physical Society,
2016, doi:10.1103/PhysRevLett.116.028102.
short: A. Callan Jones, V. Ruprecht, S. Wieser, C.-P.J. Heisenberg, R. Voituriez,
Physical Review Letters 116 (2016).
date_created: 2018-12-11T11:50:53Z
date_published: 2016-01-15T00:00:00Z
date_updated: 2021-01-12T06:49:19Z
day: '15'
department:
- _id: CaHe
doi: 10.1103/PhysRevLett.116.028102
intvolume: ' 116'
issue: '2'
language:
- iso: eng
month: '01'
oa_version: None
project:
- _id: 2529486C-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: T 560-B17
name: Cell- and Tissue Mechanics in Zebrafish Germ Layer Formation
publication: Physical Review Letters
publication_status: published
publisher: American Physical Society
publist_id: '6095'
quality_controlled: '1'
scopus_import: 1
status: public
title: Cortical flow-driven shapes of nonadherent cells
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 116
year: '2016'
...
---
_id: '1249'
abstract:
- lang: eng
text: 'Actin and myosin assemble into a thin layer of a highly dynamic network underneath
the membrane of eukaryotic cells. This network generates the forces that drive
cell- and tissue-scale morphogenetic processes. The effective material properties
of this active network determine large-scale deformations and other morphogenetic
events. For example, the characteristic time of stress relaxation (the Maxwell
time τM) in the actomyosin sets the timescale of large-scale deformation of the
cortex. Similarly, the characteristic length of stress propagation (the hydrodynamic
length λ) sets the length scale of slow deformations, and a large hydrodynamic
length is a prerequisite for long-ranged cortical flows. Here we introduce a method
to determine physical parameters of the actomyosin cortical layer in vivo directly
from laser ablation experiments. For this we investigate the cortical response
to laser ablation in the one-cell-stage Caenorhabditis elegans embryo and in the
gastrulating zebrafish embryo. These responses can be interpreted using a coarse-grained
physical description of the cortex in terms of a two-dimensional thin film of
an active viscoelastic gel. To determine the Maxwell time τM, the hydrodynamic
length λ, the ratio of active stress ζΔμ, and per-area friction γ, we evaluated
the response to laser ablation in two different ways: by quantifying flow and
density fields as a function of space and time, and by determining the time evolution
of the shape of the ablated region. Importantly, both methods provide best-fit
physical parameters that are in close agreement with each other and that are similar
to previous estimates in the two systems. Our method provides an accurate and
robust means for measuring physical parameters of the actomyosin cortical layer.
It can be useful for investigations of actomyosin mechanics at the cellular-scale,
but also for providing insights into the active mechanics processes that govern
tissue-scale morphogenesis.'
acknowledgement: S.W.G. acknowledges support by grant no. 281903 from the European
Research Council and by grant No. GR-7271/2-1 from the Deutsche Forschungsgemeinschaft.
S.W.G. and C.-P.H. acknowledge support through a grant from the Fonds zur Förderung
der Wissenschaftlichen Forschung and the Deutsche Forschungsgemeinschaft (No. I930-B20).
We are grateful to Daniel Dickinson for providing the LP133 C. elegans strain. We
thank G. Salbreux, V. K. Krishnamurthy, and J. S. Bois for fruitful discussions.
author:
- first_name: Arnab
full_name: Saha, Arnab
last_name: Saha
- first_name: Masatoshi
full_name: Nishikawa, Masatoshi
last_name: Nishikawa
- first_name: Martin
full_name: Behrndt, Martin
id: 3ECECA3A-F248-11E8-B48F-1D18A9856A87
last_name: Behrndt
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
- first_name: Frank
full_name: Julicher, Frank
last_name: Julicher
- first_name: Stephan
full_name: Grill, Stephan
last_name: Grill
citation:
ama: Saha A, Nishikawa M, Behrndt M, Heisenberg C-PJ, Julicher F, Grill S. Determining
physical properties of the cell cortex. Biophysical Journal. 2016;110(6):1421-1429.
doi:10.1016/j.bpj.2016.02.013
apa: Saha, A., Nishikawa, M., Behrndt, M., Heisenberg, C.-P. J., Julicher, F., &
Grill, S. (2016). Determining physical properties of the cell cortex. Biophysical
Journal. Biophysical Society. https://doi.org/10.1016/j.bpj.2016.02.013
chicago: Saha, Arnab, Masatoshi Nishikawa, Martin Behrndt, Carl-Philipp J Heisenberg,
Frank Julicher, and Stephan Grill. “Determining Physical Properties of the Cell
Cortex.” Biophysical Journal. Biophysical Society, 2016. https://doi.org/10.1016/j.bpj.2016.02.013.
ieee: A. Saha, M. Nishikawa, M. Behrndt, C.-P. J. Heisenberg, F. Julicher, and S.
Grill, “Determining physical properties of the cell cortex,” Biophysical Journal,
vol. 110, no. 6. Biophysical Society, pp. 1421–1429, 2016.
ista: Saha A, Nishikawa M, Behrndt M, Heisenberg C-PJ, Julicher F, Grill S. 2016.
Determining physical properties of the cell cortex. Biophysical Journal. 110(6),
1421–1429.
mla: Saha, Arnab, et al. “Determining Physical Properties of the Cell Cortex.” Biophysical
Journal, vol. 110, no. 6, Biophysical Society, 2016, pp. 1421–29, doi:10.1016/j.bpj.2016.02.013.
short: A. Saha, M. Nishikawa, M. Behrndt, C.-P.J. Heisenberg, F. Julicher, S. Grill,
Biophysical Journal 110 (2016) 1421–1429.
date_created: 2018-12-11T11:50:56Z
date_published: 2016-03-29T00:00:00Z
date_updated: 2021-01-12T06:49:23Z
day: '29'
ddc:
- '572'
- '576'
department:
- _id: CaHe
doi: 10.1016/j.bpj.2016.02.013
file:
- access_level: open_access
checksum: c408cf2e25a25c8d711cffea524bda55
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:10:54Z
date_updated: 2020-07-14T12:44:41Z
file_id: '4845'
file_name: IST-2016-706-v1+1_1-s2.0-S0006349516001582-main.pdf
file_size: 1965645
relation: main_file
file_date_updated: 2020-07-14T12:44:41Z
has_accepted_license: '1'
intvolume: ' 110'
issue: '6'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
page: 1421 - 1429
project:
- _id: 252ABD0A-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I 930-B20
name: Control of Epithelial Cell Layer Spreading in Zebrafish
publication: Biophysical Journal
publication_status: published
publisher: Biophysical Society
publist_id: '6079'
pubrep_id: '706'
quality_controlled: '1'
scopus_import: 1
status: public
title: Determining physical properties of the cell cortex
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 110
year: '2016'
...
---
_id: '1271'
abstract:
- lang: eng
text: 'Background: High directional persistence is often assumed to enhance the
efficiency of chemotactic migration. Yet, cells in vivo usually display meandering
trajectories with relatively low directional persistence, and the control and
function of directional persistence during cell migration in three-dimensional
environments are poorly understood. Results: Here, we use mesendoderm progenitors
migrating during zebrafish gastrulation as a model system to investigate the control
of directional persistence during migration in vivo. We show that progenitor cells
alternate persistent run phases with tumble phases that result in cell reorientation.
Runs are characterized by the formation of directed actin-rich protrusions and
tumbles by enhanced blebbing. Increasing the proportion of actin-rich protrusions
or blebs leads to longer or shorter run phases, respectively. Importantly, both
reducing and increasing run phases result in larger spatial dispersion of the
cells, indicative of reduced migration precision. A physical model quantitatively
recapitulating the migratory behavior of mesendoderm progenitors indicates that
the ratio of tumbling to run times, and thus the specific degree of directional
persistence of migration, are critical for optimizing migration precision. Conclusions:
Together, our experiments and model provide mechanistic insight into the control
of migration directionality for cells moving in three-dimensional environments
that combine different protrusion types, whereby the proportion of blebs to actin-rich
protrusions determines the directional persistence and precision of movement by
regulating the ratio of tumbling to run times.'
acknowledged_ssus:
- _id: LifeSc
acknowledgement: "We thank K. Lee, C. Norden, A. Webb, and the members of the Paluch
lab for\r\ncomments on the manuscript. We are grateful to P. Rørth and Peter Dieterich\r\nfor
discussions, S. Ares, Y. Arboleda-Estudillo and S. Schneider for technical help,\r\nM.
Biro for help with programming, and the BIOTEC/MPI-CBG and IST zebrafish\r\nand
imaging facilities for help and advice at various stages of this project. This work
was supported by the Max Planck Society, the Medical Research Council UK (core funding
to the MRC LMCB), and by grants from the Polish Ministry of Science and Higher Education
(454/N-MPG/2009/0) to EKP, the Deutsche Forschungsgemeinschaft (HE 3231/6-1 and
PA 1590/1-1) to CPH and EKP, a A*Star JCO career development award (12302FG010)
to WY and a Damon Runyon fellowship award to ADM (DRG 2157-12). This work was also
supported by the Francis Crick Institute which receives its core funding from Cancer
Research UK (FC001317), the UK Medical Research Council (FC001317), and the Wellcome
Trust (FC001317) to GS."
article_number: '74'
author:
- first_name: Alba
full_name: Diz Muñoz, Alba
last_name: Diz Muñoz
- first_name: Pawel
full_name: Romanczuk, Pawel
last_name: Romanczuk
- first_name: Weimiao
full_name: Yu, Weimiao
last_name: Yu
- first_name: Martin
full_name: Bergert, Martin
last_name: Bergert
- first_name: Kenzo
full_name: Ivanovitch, Kenzo
last_name: Ivanovitch
- first_name: Guillame
full_name: Salbreux, Guillame
last_name: Salbreux
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
- first_name: Ewa
full_name: Paluch, Ewa
last_name: Paluch
citation:
ama: Diz Muñoz A, Romanczuk P, Yu W, et al. Steering cell migration by alternating
blebs and actin-rich protrusions. BMC Biology. 2016;14(1). doi:10.1186/s12915-016-0294-x
apa: Diz Muñoz, A., Romanczuk, P., Yu, W., Bergert, M., Ivanovitch, K., Salbreux,
G., … Paluch, E. (2016). Steering cell migration by alternating blebs and actin-rich
protrusions. BMC Biology. BioMed Central. https://doi.org/10.1186/s12915-016-0294-x
chicago: Diz Muñoz, Alba, Pawel Romanczuk, Weimiao Yu, Martin Bergert, Kenzo Ivanovitch,
Guillame Salbreux, Carl-Philipp J Heisenberg, and Ewa Paluch. “Steering Cell Migration
by Alternating Blebs and Actin-Rich Protrusions.” BMC Biology. BioMed Central,
2016. https://doi.org/10.1186/s12915-016-0294-x.
ieee: A. Diz Muñoz et al., “Steering cell migration by alternating blebs
and actin-rich protrusions,” BMC Biology, vol. 14, no. 1. BioMed Central,
2016.
ista: Diz Muñoz A, Romanczuk P, Yu W, Bergert M, Ivanovitch K, Salbreux G, Heisenberg
C-PJ, Paluch E. 2016. Steering cell migration by alternating blebs and actin-rich
protrusions. BMC Biology. 14(1), 74.
mla: Diz Muñoz, Alba, et al. “Steering Cell Migration by Alternating Blebs and Actin-Rich
Protrusions.” BMC Biology, vol. 14, no. 1, 74, BioMed Central, 2016, doi:10.1186/s12915-016-0294-x.
short: A. Diz Muñoz, P. Romanczuk, W. Yu, M. Bergert, K. Ivanovitch, G. Salbreux,
C.-P.J. Heisenberg, E. Paluch, BMC Biology 14 (2016).
date_created: 2018-12-11T11:51:04Z
date_published: 2016-09-02T00:00:00Z
date_updated: 2021-01-12T06:49:32Z
day: '02'
ddc:
- '572'
- '576'
department:
- _id: CaHe
doi: 10.1186/s12915-016-0294-x
file:
- access_level: open_access
checksum: 0bfa484ac69a0a560fb9a4589aeda7f6
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:13:20Z
date_updated: 2020-07-14T12:44:42Z
file_id: '5002'
file_name: IST-2016-695-v1+1_s12915-016-0294-x.pdf
file_size: 1875695
relation: main_file
file_date_updated: 2020-07-14T12:44:42Z
has_accepted_license: '1'
intvolume: ' 14'
issue: '1'
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
project:
- _id: 252064B8-B435-11E9-9278-68D0E5697425
grant_number: HE_3231/6-1
name: Analysis of the Formation and Function of Different Cell Protusion Types During
Cell Migration in Vivo
publication: BMC Biology
publication_status: published
publisher: BioMed Central
publist_id: '6049'
pubrep_id: '695'
quality_controlled: '1'
scopus_import: 1
status: public
title: Steering cell migration by alternating blebs and actin-rich protrusions
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 14
year: '2016'
...
---
_id: '1275'
article_number: '139802'
author:
- first_name: Andrew
full_name: Callan Jones, Andrew
last_name: Callan Jones
- first_name: Verena
full_name: Ruprecht, Verena
id: 4D71A03A-F248-11E8-B48F-1D18A9856A87
last_name: Ruprecht
orcid: 0000-0003-4088-8633
- first_name: Stefan
full_name: Wieser, Stefan
id: 355AA5A0-F248-11E8-B48F-1D18A9856A87
last_name: Wieser
orcid: 0000-0002-2670-2217
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
- first_name: Raphaël
full_name: Voituriez, Raphaël
last_name: Voituriez
citation:
ama: Callan Jones A, Ruprecht V, Wieser S, Heisenberg C-PJ, Voituriez R. Callan-Jones
et al. Reply. Physical Review Letters. 2016;117(13). doi:10.1103/PhysRevLett.117.139802
apa: Callan Jones, A., Ruprecht, V., Wieser, S., Heisenberg, C.-P. J., & Voituriez,
R. (2016). Callan-Jones et al. Reply. Physical Review Letters. American
Physical Society. https://doi.org/10.1103/PhysRevLett.117.139802
chicago: Callan Jones, Andrew, Verena Ruprecht, Stefan Wieser, Carl-Philipp J Heisenberg,
and Raphaël Voituriez. “Callan-Jones et Al. Reply.” Physical Review Letters.
American Physical Society, 2016. https://doi.org/10.1103/PhysRevLett.117.139802.
ieee: A. Callan Jones, V. Ruprecht, S. Wieser, C.-P. J. Heisenberg, and R. Voituriez,
“Callan-Jones et al. Reply,” Physical Review Letters, vol. 117, no. 13.
American Physical Society, 2016.
ista: Callan Jones A, Ruprecht V, Wieser S, Heisenberg C-PJ, Voituriez R. 2016.
Callan-Jones et al. Reply. Physical Review Letters. 117(13), 139802.
mla: Callan Jones, Andrew, et al. “Callan-Jones et Al. Reply.” Physical Review
Letters, vol. 117, no. 13, 139802, American Physical Society, 2016, doi:10.1103/PhysRevLett.117.139802.
short: A. Callan Jones, V. Ruprecht, S. Wieser, C.-P.J. Heisenberg, R. Voituriez,
Physical Review Letters 117 (2016).
date_created: 2018-12-11T11:51:05Z
date_published: 2016-09-22T00:00:00Z
date_updated: 2021-01-12T06:49:33Z
day: '22'
department:
- _id: CaHe
doi: 10.1103/PhysRevLett.117.139802
intvolume: ' 117'
issue: '13'
language:
- iso: eng
month: '09'
oa_version: None
publication: Physical Review Letters
publication_status: published
publisher: American Physical Society
publist_id: '6041'
quality_controlled: '1'
scopus_import: 1
status: public
title: Callan-Jones et al. Reply
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 117
year: '2016'
...
---
_id: '802'
abstract:
- lang: eng
text: Glycoinositolphosphoceramides (GIPCs) are complex sphingolipids present at
the plasma membrane of various eukaryotes with the important exception of mammals.
In fungi, these glycosphingolipids commonly contain an alpha-mannose residue (Man)
linked at position 2 of the inositol. However, several pathogenic fungi additionally
synthesize zwitterionic GIPCs carrying an alpha-glucosamine residue (GlcN) at
this position. In the human pathogen Aspergillus fumigatus, the GlcNalpha1,2IPC
core (where IPC is inositolphosphoceramide) is elongated to Manalpha1,3Manalpha1,6GlcNalpha1,2IPC,
which is the most abundant GIPC synthesized by this fungus. In this study, we
identified an A. fumigatus N-acetylglucosaminyltransferase, named GntA, and demonstrate
its involvement in the initiation of zwitterionic GIPC biosynthesis. Targeted
deletion of the gene encoding GntA in A. fumigatus resulted in complete absence
of zwitterionic GIPC; a phenotype that could be reverted by episomal expression
of GntA in the mutant. The N-acetylhexosaminyltransferase activity of GntA was
substantiated by production of N-acetylhexosamine-IPC in the yeast Saccharomyces
cerevisiae upon GntA expression. Using an in vitro assay, GntA was furthermore
shown to use UDP-N-acetylglucosamine as donor substrate to generate a glycolipid
product resistant to saponification and to digestion by phosphatidylinositol-phospholipase
C as expected for GlcNAcalpha1,2IPC. Finally, as the enzymes involved in mannosylation
of IPC, GntA was localized to the Golgi apparatus, the site of IPC synthesis.
author:
- first_name: Jakob
full_name: Engel, Jakob
last_name: Engel
- first_name: Philipp S
full_name: Schmalhorst, Philipp S
id: 309D50DA-F248-11E8-B48F-1D18A9856A87
last_name: Schmalhorst
orcid: 0000-0002-5795-0133
- first_name: Anke
full_name: Kruger, Anke
last_name: Kruger
- first_name: Christina
full_name: Muller, Christina
last_name: Muller
- first_name: Falk
full_name: Buettner, Falk
last_name: Buettner
- first_name: Françoise
full_name: Routier, Françoise
last_name: Routier
citation:
ama: Engel J, Schmalhorst PS, Kruger A, Muller C, Buettner F, Routier F. Characterization
of an N-acetylglucosaminyltransferase involved in Aspergillus fumigatus zwitterionic
glycoinositolphosphoceramide biosynthesis. Glycobiology. 2015;25(12):1423-1430.
doi:10.1093/glycob/cwv059
apa: Engel, J., Schmalhorst, P. S., Kruger, A., Muller, C., Buettner, F., &
Routier, F. (2015). Characterization of an N-acetylglucosaminyltransferase involved
in Aspergillus fumigatus zwitterionic glycoinositolphosphoceramide biosynthesis.
Glycobiology. Oxford University Press. https://doi.org/10.1093/glycob/cwv059
chicago: Engel, Jakob, Philipp S Schmalhorst, Anke Kruger, Christina Muller, Falk
Buettner, and Françoise Routier. “Characterization of an N-Acetylglucosaminyltransferase
Involved in Aspergillus Fumigatus Zwitterionic Glycoinositolphosphoceramide Biosynthesis.”
Glycobiology. Oxford University Press, 2015. https://doi.org/10.1093/glycob/cwv059.
ieee: J. Engel, P. S. Schmalhorst, A. Kruger, C. Muller, F. Buettner, and F. Routier,
“Characterization of an N-acetylglucosaminyltransferase involved in Aspergillus
fumigatus zwitterionic glycoinositolphosphoceramide biosynthesis,” Glycobiology,
vol. 25, no. 12. Oxford University Press, pp. 1423–1430, 2015.
ista: Engel J, Schmalhorst PS, Kruger A, Muller C, Buettner F, Routier F. 2015.
Characterization of an N-acetylglucosaminyltransferase involved in Aspergillus
fumigatus zwitterionic glycoinositolphosphoceramide biosynthesis. Glycobiology.
25(12), 1423–1430.
mla: Engel, Jakob, et al. “Characterization of an N-Acetylglucosaminyltransferase
Involved in Aspergillus Fumigatus Zwitterionic Glycoinositolphosphoceramide Biosynthesis.”
Glycobiology, vol. 25, no. 12, Oxford University Press, 2015, pp. 1423–30,
doi:10.1093/glycob/cwv059.
short: J. Engel, P.S. Schmalhorst, A. Kruger, C. Muller, F. Buettner, F. Routier,
Glycobiology 25 (2015) 1423–1430.
date_created: 2018-12-11T11:48:35Z
date_published: 2015-12-01T00:00:00Z
date_updated: 2021-01-12T08:16:33Z
day: '01'
department:
- _id: CaHe
doi: 10.1093/glycob/cwv059
external_id:
pmid:
- '26306635'
intvolume: ' 25'
issue: '12'
language:
- iso: eng
month: '12'
oa_version: None
page: 1423 - 1430
pmid: 1
publication: Glycobiology
publication_status: published
publisher: Oxford University Press
publist_id: '6851'
quality_controlled: '1'
scopus_import: 1
status: public
title: Characterization of an N-acetylglucosaminyltransferase involved in Aspergillus
fumigatus zwitterionic glycoinositolphosphoceramide biosynthesis
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 25
year: '2015'
...
---
_id: '1817'
abstract:
- lang: eng
text: 'Vertebrates have a unique 3D body shape in which correct tissue and organ
shape and alignment are essential for function. For example, vision requires the
lens to be centred in the eye cup which must in turn be correctly positioned in
the head. Tissue morphogenesis depends on force generation, force transmission
through the tissue, and response of tissues and extracellular matrix to force.
Although a century ago D''Arcy Thompson postulated that terrestrial animal body
shapes are conditioned by gravity, there has been no animal model directly demonstrating
how the aforementioned mechano-morphogenetic processes are coordinated to generate
a body shape that withstands gravity. Here we report a unique medaka fish (Oryzias
latipes) mutant, hirame (hir), which is sensitive to deformation by gravity. hir
embryos display a markedly flattened body caused by mutation of YAP, a nuclear
executor of Hippo signalling that regulates organ size. We show that actomyosin-mediated
tissue tension is reduced in hir embryos, leading to tissue flattening and tissue
misalignment, both of which contribute to body flattening. By analysing YAP function
in 3D spheroids of human cells, we identify the Rho GTPase activating protein
ARHGAP18 as an effector of YAP in controlling tissue tension. Together, these
findings reveal a previously unrecognised function of YAP in regulating tissue
shape and alignment required for proper 3D body shape. Understanding this morphogenetic
function of YAP could facilitate the use of embryonic stem cells to generate complex
organs requiring correct alignment of multiple tissues. '
author:
- first_name: Sean
full_name: Porazinski, Sean
last_name: Porazinski
- first_name: Huijia
full_name: Wang, Huijia
last_name: Wang
- first_name: Yoichi
full_name: Asaoka, Yoichi
last_name: Asaoka
- first_name: Martin
full_name: Behrndt, Martin
id: 3ECECA3A-F248-11E8-B48F-1D18A9856A87
last_name: Behrndt
- first_name: Tatsuo
full_name: Miyamoto, Tatsuo
last_name: Miyamoto
- first_name: Hitoshi
full_name: Morita, Hitoshi
id: 4C6E54C6-F248-11E8-B48F-1D18A9856A87
last_name: Morita
- first_name: Shoji
full_name: Hata, Shoji
last_name: Hata
- first_name: Takashi
full_name: Sasaki, Takashi
last_name: Sasaki
- first_name: Gabriel
full_name: Krens, Gabriel
id: 2B819732-F248-11E8-B48F-1D18A9856A87
last_name: Krens
orcid: 0000-0003-4761-5996
- first_name: Yumi
full_name: Osada, Yumi
last_name: Osada
- first_name: Satoshi
full_name: Asaka, Satoshi
last_name: Asaka
- first_name: Akihiro
full_name: Momoi, Akihiro
last_name: Momoi
- first_name: Sarah
full_name: Linton, Sarah
last_name: Linton
- first_name: Joel
full_name: Miesfeld, Joel
last_name: Miesfeld
- first_name: Brian
full_name: Link, Brian
last_name: Link
- first_name: Takeshi
full_name: Senga, Takeshi
last_name: Senga
- first_name: Atahualpa
full_name: Castillo Morales, Atahualpa
last_name: Castillo Morales
- first_name: Araxi
full_name: Urrutia, Araxi
last_name: Urrutia
- first_name: Nobuyoshi
full_name: Shimizu, Nobuyoshi
last_name: Shimizu
- first_name: Hideaki
full_name: Nagase, Hideaki
last_name: Nagase
- first_name: Shinya
full_name: Matsuura, Shinya
last_name: Matsuura
- first_name: Stefan
full_name: Bagby, Stefan
last_name: Bagby
- first_name: Hisato
full_name: Kondoh, Hisato
last_name: Kondoh
- first_name: Hiroshi
full_name: Nishina, Hiroshi
last_name: Nishina
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
- first_name: Makoto
full_name: Furutani Seiki, Makoto
last_name: Furutani Seiki
citation:
ama: Porazinski S, Wang H, Asaoka Y, et al. YAP is essential for tissue tension
to ensure vertebrate 3D body shape. Nature. 2015;521(7551):217-221. doi:10.1038/nature14215
apa: Porazinski, S., Wang, H., Asaoka, Y., Behrndt, M., Miyamoto, T., Morita, H.,
… Furutani Seiki, M. (2015). YAP is essential for tissue tension to ensure vertebrate
3D body shape. Nature. Nature Publishing Group. https://doi.org/10.1038/nature14215
chicago: Porazinski, Sean, Huijia Wang, Yoichi Asaoka, Martin Behrndt, Tatsuo Miyamoto,
Hitoshi Morita, Shoji Hata, et al. “YAP Is Essential for Tissue Tension to Ensure
Vertebrate 3D Body Shape.” Nature. Nature Publishing Group, 2015. https://doi.org/10.1038/nature14215.
ieee: S. Porazinski et al., “YAP is essential for tissue tension to ensure
vertebrate 3D body shape,” Nature, vol. 521, no. 7551. Nature Publishing
Group, pp. 217–221, 2015.
ista: Porazinski S, Wang H, Asaoka Y, Behrndt M, Miyamoto T, Morita H, Hata S, Sasaki
T, Krens G, Osada Y, Asaka S, Momoi A, Linton S, Miesfeld J, Link B, Senga T,
Castillo Morales A, Urrutia A, Shimizu N, Nagase H, Matsuura S, Bagby S, Kondoh
H, Nishina H, Heisenberg C-PJ, Furutani Seiki M. 2015. YAP is essential for tissue
tension to ensure vertebrate 3D body shape. Nature. 521(7551), 217–221.
mla: Porazinski, Sean, et al. “YAP Is Essential for Tissue Tension to Ensure Vertebrate
3D Body Shape.” Nature, vol. 521, no. 7551, Nature Publishing Group, 2015,
pp. 217–21, doi:10.1038/nature14215.
short: S. Porazinski, H. Wang, Y. Asaoka, M. Behrndt, T. Miyamoto, H. Morita, S.
Hata, T. Sasaki, G. Krens, Y. Osada, S. Asaka, A. Momoi, S. Linton, J. Miesfeld,
B. Link, T. Senga, A. Castillo Morales, A. Urrutia, N. Shimizu, H. Nagase, S.
Matsuura, S. Bagby, H. Kondoh, H. Nishina, C.-P.J. Heisenberg, M. Furutani Seiki,
Nature 521 (2015) 217–221.
date_created: 2018-12-11T11:54:10Z
date_published: 2015-03-16T00:00:00Z
date_updated: 2021-01-12T06:53:23Z
day: '16'
department:
- _id: CaHe
doi: 10.1038/nature14215
external_id:
pmid:
- '25778702'
intvolume: ' 521'
issue: '7551'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4720436/
month: '03'
oa: 1
oa_version: Submitted Version
page: 217 - 221
pmid: 1
publication: Nature
publication_status: published
publisher: Nature Publishing Group
publist_id: '5289'
quality_controlled: '1'
scopus_import: 1
status: public
title: YAP is essential for tissue tension to ensure vertebrate 3D body shape
type: journal_article
user_id: 2EBD1598-F248-11E8-B48F-1D18A9856A87
volume: 521
year: '2015'
...
---
_id: '1537'
abstract:
- lang: eng
text: 3D amoeboid cell migration is central to many developmental and disease-related
processes such as cancer metastasis. Here, we identify a unique prototypic amoeboid
cell migration mode in early zebrafish embryos, termed stable-bleb migration.
Stable-bleb cells display an invariant polarized balloon-like shape with exceptional
migration speed and persistence. Progenitor cells can be reversibly transformed
into stable-bleb cells irrespective of their primary fate and motile characteristics
by increasing myosin II activity through biochemical or mechanical stimuli. Using
a combination of theory and experiments, we show that, in stable-bleb cells, cortical
contractility fluctuations trigger a stochastic switch into amoeboid motility,
and a positive feedback between cortical flows and gradients in contractility
maintains stable-bleb cell polarization. We further show that rearward cortical
flows drive stable-bleb cell migration in various adhesive and non-adhesive environments,
unraveling a highly versatile amoeboid migration phenotype.
acknowledged_ssus:
- _id: SSU
acknowledgement: 'We would like to thank R. Hausschild and E. Papusheva for technical
assistance and the service facilities at the IST Austria for continuous support.
The caRhoA plasmid was a kind gift of T. Kudoh and A. Takesono. We thank M. Piel
and E. Paluch for exchanging unpublished data. '
author:
- first_name: Verena
full_name: Ruprecht, Verena
id: 4D71A03A-F248-11E8-B48F-1D18A9856A87
last_name: Ruprecht
orcid: 0000-0003-4088-8633
- first_name: Stefan
full_name: Wieser, Stefan
id: 355AA5A0-F248-11E8-B48F-1D18A9856A87
last_name: Wieser
orcid: 0000-0002-2670-2217
- first_name: Andrew
full_name: Callan Jones, Andrew
last_name: Callan Jones
- first_name: Michael
full_name: Smutny, Michael
id: 3FE6E4E8-F248-11E8-B48F-1D18A9856A87
last_name: Smutny
orcid: 0000-0002-5920-9090
- first_name: Hitoshi
full_name: Morita, Hitoshi
id: 4C6E54C6-F248-11E8-B48F-1D18A9856A87
last_name: Morita
- first_name: Keisuke
full_name: Sako, Keisuke
id: 3BED66BE-F248-11E8-B48F-1D18A9856A87
last_name: Sako
orcid: 0000-0002-6453-8075
- first_name: Vanessa
full_name: Barone, Vanessa
id: 419EECCC-F248-11E8-B48F-1D18A9856A87
last_name: Barone
orcid: 0000-0003-2676-3367
- first_name: Monika
full_name: Ritsch Marte, Monika
last_name: Ritsch Marte
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
- first_name: Raphaël
full_name: Voituriez, Raphaël
last_name: Voituriez
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Ruprecht V, Wieser S, Callan Jones A, et al. Cortical contractility triggers
a stochastic switch to fast amoeboid cell motility. Cell. 2015;160(4):673-685.
doi:10.1016/j.cell.2015.01.008
apa: Ruprecht, V., Wieser, S., Callan Jones, A., Smutny, M., Morita, H., Sako, K.,
… Heisenberg, C.-P. J. (2015). Cortical contractility triggers a stochastic switch
to fast amoeboid cell motility. Cell. Cell Press. https://doi.org/10.1016/j.cell.2015.01.008
chicago: Ruprecht, Verena, Stefan Wieser, Andrew Callan Jones, Michael Smutny, Hitoshi
Morita, Keisuke Sako, Vanessa Barone, et al. “Cortical Contractility Triggers
a Stochastic Switch to Fast Amoeboid Cell Motility.” Cell. Cell Press,
2015. https://doi.org/10.1016/j.cell.2015.01.008.
ieee: V. Ruprecht et al., “Cortical contractility triggers a stochastic switch
to fast amoeboid cell motility,” Cell, vol. 160, no. 4. Cell Press, pp.
673–685, 2015.
ista: Ruprecht V, Wieser S, Callan Jones A, Smutny M, Morita H, Sako K, Barone V,
Ritsch Marte M, Sixt MK, Voituriez R, Heisenberg C-PJ. 2015. Cortical contractility
triggers a stochastic switch to fast amoeboid cell motility. Cell. 160(4), 673–685.
mla: Ruprecht, Verena, et al. “Cortical Contractility Triggers a Stochastic Switch
to Fast Amoeboid Cell Motility.” Cell, vol. 160, no. 4, Cell Press, 2015,
pp. 673–85, doi:10.1016/j.cell.2015.01.008.
short: V. Ruprecht, S. Wieser, A. Callan Jones, M. Smutny, H. Morita, K. Sako, V.
Barone, M. Ritsch Marte, M.K. Sixt, R. Voituriez, C.-P.J. Heisenberg, Cell 160
(2015) 673–685.
date_created: 2018-12-11T11:52:35Z
date_published: 2015-02-12T00:00:00Z
date_updated: 2023-09-07T12:05:08Z
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doi: 10.1016/j.cell.2015.01.008
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title: Cortical contractility triggers a stochastic switch to fast amoeboid cell motility
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