---
_id: '3791'
abstract:
- lang: eng
text: During the development of multicellular organisms, cell fate specification
is followed by the sorting of different cell types into distinct domains from
where the different tissues and organs are formed. Cell sorting involves both
the segregation of a mixed population of cells with different fates and properties
into distinct domains, and the active maintenance of their segregated state. Because
of its biological importance and apparent resemblance to fluid segregation in
physics, cell sorting was extensively studied by both biologists and physicists
over the last decades. Different theories were developed that try to explain cell
sorting on the basis of the physical properties of the constituent cells. However,
only recently the molecular and cellular mechanisms that control the physical
properties driving cell sorting, have begun to be unraveled. In this review, we
will provide an overview of different cell-sorting processes in development and
discuss how these processes can be explained by the different sorting theories,
and how these theories in turn can be connected to the molecular and cellular
mechanisms driving these processes.
alternative_title:
- Current Topics in Developmental Biology
article_processing_charge: No
author:
- first_name: Gabriel
full_name: Krens, Gabriel
id: 2B819732-F248-11E8-B48F-1D18A9856A87
last_name: Krens
orcid: 0000-0003-4761-5996
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: 'Krens G, Heisenberg C-PJ. Cell sorting in development. In: Labouesse M, ed.
Forces and Tension in Development. Vol 95. Elsevier; 2011:189-213. doi:10.1016/B978-0-12-385065-2.00006-2'
apa: Krens, G., & Heisenberg, C.-P. J. (2011). Cell sorting in development.
In M. Labouesse (Ed.), Forces and Tension in Development (Vol. 95, pp.
189–213). Elsevier. https://doi.org/10.1016/B978-0-12-385065-2.00006-2
chicago: Krens, Gabriel, and Carl-Philipp J Heisenberg. “Cell Sorting in Development.”
In Forces and Tension in Development, edited by Michel Labouesse, 95:189–213.
Elsevier, 2011. https://doi.org/10.1016/B978-0-12-385065-2.00006-2.
ieee: G. Krens and C.-P. J. Heisenberg, “Cell sorting in development,” in Forces
and Tension in Development, vol. 95, M. Labouesse, Ed. Elsevier, 2011, pp.
189–213.
ista: 'Krens G, Heisenberg C-PJ. 2011.Cell sorting in development. In: Forces and
Tension in Development. Current Topics in Developmental Biology, vol. 95, 189–213.'
mla: Krens, Gabriel, and Carl-Philipp J. Heisenberg. “Cell Sorting in Development.”
Forces and Tension in Development, edited by Michel Labouesse, vol. 95,
Elsevier, 2011, pp. 189–213, doi:10.1016/B978-0-12-385065-2.00006-2.
short: G. Krens, C.-P.J. Heisenberg, in:, M. Labouesse (Ed.), Forces and Tension
in Development, Elsevier, 2011, pp. 189–213.
date_created: 2018-12-11T12:05:11Z
date_published: 2011-01-01T00:00:00Z
date_updated: 2021-01-12T07:52:13Z
day: '01'
department:
- _id: CaHe
doi: 10.1016/B978-0-12-385065-2.00006-2
editor:
- first_name: Michel
full_name: Labouesse, Michel
last_name: Labouesse
intvolume: ' 95'
language:
- iso: eng
month: '01'
oa_version: None
page: 189 - 213
publication: Forces and Tension in Development
publication_status: published
publisher: Elsevier
publist_id: '2436'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Cell sorting in development
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 95
year: '2011'
...
---
_id: '3788'
abstract:
- lang: eng
text: Cell sorting is a widespread phenomenon pivotal to the early development of
multicellular organisms. In vitro cell sorting studies have been instrumental
in revealing the cellular properties driving this process. However, these studies
have as yet been limited to two-dimensional analysis of three-dimensional cell
sorting events. Here we describe a method to record the sorting of primary zebrafish
ectoderm and mesoderm germ layer progenitor cells in three dimensions over time,
and quantitatively analyze their sorting behavior using an order parameter related
to heterotypic interface length. We investigate the cell population size dependence
of sorted aggregates and find that the germ layer progenitor cells engulfed in
the final configuration display a relationship between total interfacial length
and system size according to a simple geometrical argument, subject to a finite-size
effect.
author:
- first_name: Abigail
full_name: Klopper, Abigail
last_name: Klopper
- first_name: Gabriel
full_name: Krens, Gabriel
id: 2B819732-F248-11E8-B48F-1D18A9856A87
last_name: Krens
orcid: 0000-0003-4761-5996
- first_name: Stephan
full_name: Grill, Stephan
last_name: Grill
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: 'Klopper A, Krens G, Grill S, Heisenberg C-PJ. Finite-size corrections to scaling
behavior in sorted cell aggregates. The European Physical Journal E: Soft Matter
and Biological Physics. 2010;33(2):99-103. doi:10.1140/epje/i2010-10642-y'
apa: 'Klopper, A., Krens, G., Grill, S., & Heisenberg, C.-P. J. (2010). Finite-size
corrections to scaling behavior in sorted cell aggregates. The European Physical
Journal E: Soft Matter and Biological Physics. Springer. https://doi.org/10.1140/epje/i2010-10642-y'
chicago: 'Klopper, Abigail, Gabriel Krens, Stephan Grill, and Carl-Philipp J Heisenberg.
“Finite-Size Corrections to Scaling Behavior in Sorted Cell Aggregates.” The
European Physical Journal E: Soft Matter and Biological Physics. Springer,
2010. https://doi.org/10.1140/epje/i2010-10642-y.'
ieee: 'A. Klopper, G. Krens, S. Grill, and C.-P. J. Heisenberg, “Finite-size corrections
to scaling behavior in sorted cell aggregates,” The European Physical Journal
E: Soft Matter and Biological Physics, vol. 33, no. 2. Springer, pp. 99–103,
2010.'
ista: 'Klopper A, Krens G, Grill S, Heisenberg C-PJ. 2010. Finite-size corrections
to scaling behavior in sorted cell aggregates. The European Physical Journal E:
Soft Matter and Biological Physics. 33(2), 99–103.'
mla: 'Klopper, Abigail, et al. “Finite-Size Corrections to Scaling Behavior in Sorted
Cell Aggregates.” The European Physical Journal E: Soft Matter and Biological
Physics, vol. 33, no. 2, Springer, 2010, pp. 99–103, doi:10.1140/epje/i2010-10642-y.'
short: 'A. Klopper, G. Krens, S. Grill, C.-P.J. Heisenberg, The European Physical
Journal E: Soft Matter and Biological Physics 33 (2010) 99–103.'
date_created: 2018-12-11T12:05:10Z
date_published: 2010-09-18T00:00:00Z
date_updated: 2021-01-12T07:52:12Z
day: '18'
department:
- _id: CaHe
doi: 10.1140/epje/i2010-10642-y
intvolume: ' 33'
issue: '2'
language:
- iso: eng
month: '09'
oa_version: None
page: 99 - 103
publication: 'The European Physical Journal E: Soft Matter and Biological Physics'
publication_status: published
publisher: Springer
publist_id: '2439'
scopus_import: 1
status: public
title: Finite-size corrections to scaling behavior in sorted cell aggregates
type: journal_article
user_id: 2EBD1598-F248-11E8-B48F-1D18A9856A87
volume: 33
year: '2010'
...
---
_id: '3789'
abstract:
- lang: eng
text: 'The development of multicellular organisms is dependent on the tight coordination
between tissue growth and morphogenesis. The stereotypical orientation of cell
divisions has been proposed to be a fundamental mechanism by which proliferating
and growing tissues take shape. However, the actual contribution of stereotypical
division orientation (SDO) to tissue morphogenesis is unclear. In zebrafish, cell
divisions with stereotypical orientation have been implicated in both body-axis
elongation and neural rod formation [1, 2], although there is little direct evidence
for a critical function of SDO in either of these processes. Here we show that
SDO is required for formation of the neural rod midline during neurulation but
dispensable for elongation of the body axis during gastrulation. Our data indicate
that SDO during both gastrulation and neurulation is dependent on the noncanonical
Wnt receptor Frizzled 7 (Fz7) and that interfering with cell division orientation
leads to severe defects in neural rod midline formation but not body-axis elongation.
These findings suggest a novel function for Fz7-controlled cell division orientation
in neural rod midline formation during neurulation. '
acknowledgement: "This work was supported by grants from the Fundacion Caja Madrid
to E.Q.H. and the Institute of Science and Technology Austria, the Max-Planck-Society,
and the Deutsche Forschungsgemeinschaft to C.P.H.\r\nWe are grateful to Jon Clarke,
Andy Oates, and Garrett Greenan for reading earlier versions of this manuscript.
We thank J. Peychl, H. Ibarra, and P. Pitrone for excellent assistance and advice
in multi-photon microscopy and D. White for assistance during the image-processing
steps. We also thank D. Panhans for technical assistance, the whole Heisenberg laboratory
for useful comments and discussions, and E. Lehmann, J. Hückmann, and G. Junghans
for excellent fish care. "
author:
- first_name: Elena
full_name: Quesada-Hernández, Elena
id: EA35229E-E909-11E9-8DF8-C90C5D5AF86E
last_name: Quesada-Hernández
- first_name: Luca
full_name: Caneparo, Luca
last_name: Caneparo
- first_name: Sylvia
full_name: Schneider, Sylvia
id: 1FAC36B0-E90A-11E9-9D2F-EF31CE0C9C2F
last_name: Schneider
- first_name: Sylke
full_name: Winkler, Sylke
last_name: Winkler
- first_name: Michael
full_name: Liebling, Michael
last_name: Liebling
- first_name: Scott
full_name: Fraser, Scott
last_name: Fraser
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Quesada-Hernández E, Caneparo L, Schneider S, et al. Stereotypical cell division
orientation controls neural rod midline formation in zebrafish. Current Biology.
2010;20(21):1966-1972. doi:10.1016/j.cub.2010.10.009
apa: Quesada-Hernández, E., Caneparo, L., Schneider, S., Winkler, S., Liebling,
M., Fraser, S., & Heisenberg, C.-P. J. (2010). Stereotypical cell division
orientation controls neural rod midline formation in zebrafish. Current Biology.
Cell Press. https://doi.org/10.1016/j.cub.2010.10.009
chicago: Quesada-Hernández, Elena, Luca Caneparo, Sylvia Schneider, Sylke Winkler,
Michael Liebling, Scott Fraser, and Carl-Philipp J Heisenberg. “Stereotypical
Cell Division Orientation Controls Neural Rod Midline Formation in Zebrafish.”
Current Biology. Cell Press, 2010. https://doi.org/10.1016/j.cub.2010.10.009.
ieee: E. Quesada-Hernández et al., “Stereotypical cell division orientation
controls neural rod midline formation in zebrafish,” Current Biology, vol.
20, no. 21. Cell Press, pp. 1966–1972, 2010.
ista: Quesada-Hernández E, Caneparo L, Schneider S, Winkler S, Liebling M, Fraser
S, Heisenberg C-PJ. 2010. Stereotypical cell division orientation controls neural
rod midline formation in zebrafish. Current Biology. 20(21), 1966–1972.
mla: Quesada-Hernández, Elena, et al. “Stereotypical Cell Division Orientation Controls
Neural Rod Midline Formation in Zebrafish.” Current Biology, vol. 20, no.
21, Cell Press, 2010, pp. 1966–72, doi:10.1016/j.cub.2010.10.009.
short: E. Quesada-Hernández, L. Caneparo, S. Schneider, S. Winkler, M. Liebling,
S. Fraser, C.-P.J. Heisenberg, Current Biology 20 (2010) 1966–1972.
date_created: 2018-12-11T12:05:11Z
date_published: 2010-11-09T00:00:00Z
date_updated: 2021-01-12T07:52:12Z
day: '09'
department:
- _id: CaHe
doi: 10.1016/j.cub.2010.10.009
intvolume: ' 20'
issue: '21'
language:
- iso: eng
month: '11'
oa_version: None
page: 1966 - 1972
publication: Current Biology
publication_status: published
publisher: Cell Press
publist_id: '2438'
quality_controlled: '1'
scopus_import: 1
status: public
title: Stereotypical cell division orientation controls neural rod midline formation
in zebrafish
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 20
year: '2010'
...
---
_id: '3790'
abstract:
- lang: eng
text: Cell shape and motility are primarily controlled by cellular mechanics. The
attachment of the plasma membrane to the underlying actomyosin cortex has been
proposed to be important for cellular processes involving membrane deformation.
However, little is known about the actual function of membrane-to-cortex attachment
(MCA) in cell protrusion formation and migration, in particular in the context
of the developing embryo. Here, we use a multidisciplinary approach to study MCA
in zebrafish mesoderm and endoderm (mesendoderm) germ layer progenitor cells,
which migrate using a combination of different protrusion types, namely, lamellipodia,
filopodia, and blebs, during zebrafish gastrulation. By interfering with the activity
of molecules linking the cortex to the membrane and measuring resulting changes
in MCA by atomic force microscopy, we show that reducing MCA in mesendoderm progenitors
increases the proportion of cellular blebs and reduces the directionality of cell
migration. We propose that MCA is a key parameter controlling the relative proportions
of different cell protrusion types in mesendoderm progenitors, and thus is key
in controlling directed migration during gastrulation.
acknowledgement: "We would like to thank A. G. Clark, S. Grill, A. Oates, E. Raz,
L. Rohde, and M. Zerial for reading earlier versions of the manuscript. We are grateful
to W. Zachariae, Y. Arboleda-Estudillo, S. Schneider, P. Stockinger, D. Panhans,
M. Biro, J. C. Olaya, and the BIOTEC/MPI-CBG zebrafish and imaging facilities for
help and advice at various stages of this project and to J. Helenius for help with
programming. This work was supported by grants from the Boehringer Ingelheim Fonds
to MK, the Polish Ministry of Science and Higher Education to E. P., and the Deutsche
Forschungsgemeinschaft (HE 3231/6-1 and PA 1590/1-1) to CPH and EP.\r\n"
article_number: e1000544
author:
- first_name: Alba
full_name: Diz Muñoz, Alba
last_name: Diz Muñoz
- first_name: Michael
full_name: Krieg, Michael
last_name: Krieg
- first_name: Martin
full_name: Bergert, Martin
last_name: Bergert
- first_name: Itziar
full_name: Ibarlucea Benitez, Itziar
last_name: Ibarlucea Benitez
- first_name: Daniel
full_name: Müller, Daniel
last_name: Müller
- first_name: Ewa
full_name: Paluch, Ewa
last_name: Paluch
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Diz Muñoz A, Krieg M, Bergert M, et al. Control of directed cell migration
in vivo by membrane-to-cortex attachment. PLoS Biology. 2010;8(11). doi:10.1371/journal.pbio.1000544
apa: Diz Muñoz, A., Krieg, M., Bergert, M., Ibarlucea Benitez, I., Müller, D., Paluch,
E., & Heisenberg, C.-P. J. (2010). Control of directed cell migration in vivo
by membrane-to-cortex attachment. PLoS Biology. Public Library of Science.
https://doi.org/10.1371/journal.pbio.1000544
chicago: Diz Muñoz, Alba, Michael Krieg, Martin Bergert, Itziar Ibarlucea Benitez,
Daniel Müller, Ewa Paluch, and Carl-Philipp J Heisenberg. “Control of Directed
Cell Migration in Vivo by Membrane-to-Cortex Attachment.” PLoS Biology.
Public Library of Science, 2010. https://doi.org/10.1371/journal.pbio.1000544.
ieee: A. Diz Muñoz et al., “Control of directed cell migration in vivo by
membrane-to-cortex attachment,” PLoS Biology, vol. 8, no. 11. Public Library
of Science, 2010.
ista: Diz Muñoz A, Krieg M, Bergert M, Ibarlucea Benitez I, Müller D, Paluch E,
Heisenberg C-PJ. 2010. Control of directed cell migration in vivo by membrane-to-cortex
attachment. PLoS Biology. 8(11), e1000544.
mla: Diz Muñoz, Alba, et al. “Control of Directed Cell Migration in Vivo by Membrane-to-Cortex
Attachment.” PLoS Biology, vol. 8, no. 11, e1000544, Public Library of
Science, 2010, doi:10.1371/journal.pbio.1000544.
short: A. Diz Muñoz, M. Krieg, M. Bergert, I. Ibarlucea Benitez, D. Müller, E. Paluch,
C.-P.J. Heisenberg, PLoS Biology 8 (2010).
date_created: 2018-12-11T12:05:11Z
date_published: 2010-11-30T00:00:00Z
date_updated: 2021-01-12T07:52:13Z
day: '30'
ddc:
- '576'
department:
- _id: CaHe
doi: 10.1371/journal.pbio.1000544
file:
- access_level: open_access
checksum: 52d18c90ca6b02234cea5e8b399b7f46
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:08:24Z
date_updated: 2020-07-14T12:46:16Z
file_id: '4685'
file_name: IST-2015-365-v1+1_journal.pbio.1000544.pdf
file_size: 799506
relation: main_file
file_date_updated: 2020-07-14T12:46:16Z
has_accepted_license: '1'
intvolume: ' 8'
issue: '11'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '11'
oa: 1
oa_version: Published Version
publication: PLoS Biology
publication_status: published
publisher: Public Library of Science
publist_id: '2437'
pubrep_id: '365'
quality_controlled: '1'
scopus_import: 1
status: public
title: Control of directed cell migration in vivo by membrane-to-cortex attachment
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 8
year: '2010'
...
---
_id: '3962'
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Holger
full_name: Pflicke, Holger
id: CAA57A9A-5B61-11E9-B130-E0C1E1F2C83D
last_name: Pflicke
citation:
ama: Pflicke H. Dendritic cell migration across basement membranes in the skin.
2010.
apa: Pflicke, H. (2010). Dendritic cell migration across basement membranes
in the skin. Institute of Science and Technology Austria.
chicago: Pflicke, Holger. “ Dendritic Cell Migration across Basement Membranes
in the Skin.” Institute of Science and Technology Austria, 2010.
ieee: H. Pflicke, “ Dendritic cell migration across basement membranes in the skin,”
Institute of Science and Technology Austria, 2010.
ista: Pflicke H. 2010. Dendritic cell migration across basement membranes in the
skin. Institute of Science and Technology Austria.
mla: Pflicke, Holger. Dendritic Cell Migration across Basement Membranes in
the Skin. Institute of Science and Technology Austria, 2010.
short: H. Pflicke, Dendritic Cell Migration across Basement Membranes in the Skin,
Institute of Science and Technology Austria, 2010.
date_created: 2018-12-11T12:06:08Z
date_published: 2010-07-01T00:00:00Z
date_updated: 2023-09-07T11:28:47Z
day: '01'
degree_awarded: PhD
department:
- _id: CaHe
- _id: GradSch
language:
- iso: eng
month: '07'
oa_version: None
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
publist_id: '2165'
status: public
supervisor:
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
title: "\uFEFF\uFEFFDendritic cell migration across basement membranes in the skin"
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2010'
...
---
_id: '4157'
abstract:
- lang: eng
text: Integrin- and cadherin-mediated adhesion is central for cell and tissue morphogenesis,
allowing cells and tissues to change shape without loosing integrity. Studies
predominantly in cell culture showed that mechanosensation through adhesion structures
is achieved by force-mediated modulation of their molecular composition. The specific
molecular composition of adhesion sites in turn determines their signalling activity
and dynamic reorganization. Here, we will review how adhesion sites respond to
mecanical stimuli, and how spatially and temporally regulated signalling from
different adhesion sites controls cell migration and tissue morphogenesis.
acknowledged_ssus:
- _id: Bio
author:
- first_name: Ekaterina
full_name: Papusheva, Ekaterina
id: 41DB591E-F248-11E8-B48F-1D18A9856A87
last_name: Papusheva
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: 'Papusheva E, Heisenberg C-PJ. Spatial organization of adhesion: force-dependent
regulation and function in tissue morphogenesis. EMBO Journal. 2010;29(16):2753-2768.
doi:10.1038/emboj.2010.182'
apa: 'Papusheva, E., & Heisenberg, C.-P. J. (2010). Spatial organization of
adhesion: force-dependent regulation and function in tissue morphogenesis. EMBO
Journal. Wiley-Blackwell. https://doi.org/10.1038/emboj.2010.182'
chicago: 'Papusheva, Ekaterina, and Carl-Philipp J Heisenberg. “Spatial Organization
of Adhesion: Force-Dependent Regulation and Function in Tissue Morphogenesis.”
EMBO Journal. Wiley-Blackwell, 2010. https://doi.org/10.1038/emboj.2010.182.'
ieee: 'E. Papusheva and C.-P. J. Heisenberg, “Spatial organization of adhesion:
force-dependent regulation and function in tissue morphogenesis,” EMBO Journal,
vol. 29, no. 16. Wiley-Blackwell, pp. 2753–2768, 2010.'
ista: 'Papusheva E, Heisenberg C-PJ. 2010. Spatial organization of adhesion: force-dependent
regulation and function in tissue morphogenesis. EMBO Journal. 29(16), 2753–2768.'
mla: 'Papusheva, Ekaterina, and Carl-Philipp J. Heisenberg. “Spatial Organization
of Adhesion: Force-Dependent Regulation and Function in Tissue Morphogenesis.”
EMBO Journal, vol. 29, no. 16, Wiley-Blackwell, 2010, pp. 2753–68, doi:10.1038/emboj.2010.182.'
short: E. Papusheva, C.-P.J. Heisenberg, EMBO Journal 29 (2010) 2753–2768.
date_created: 2018-12-11T12:07:17Z
date_published: 2010-08-18T00:00:00Z
date_updated: 2021-01-12T07:54:55Z
day: '18'
department:
- _id: Bio
- _id: CaHe
doi: 10.1038/emboj.2010.182
external_id:
pmid:
- '20717145'
intvolume: ' 29'
issue: '16'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2924654/
month: '08'
oa: 1
oa_version: Submitted Version
page: 2753 - 2768
pmid: 1
publication: EMBO Journal
publication_status: published
publisher: Wiley-Blackwell
publist_id: '1962'
quality_controlled: '1'
scopus_import: 1
status: public
title: 'Spatial organization of adhesion: force-dependent regulation and function
in tissue morphogenesis'
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 29
year: '2010'
...