[{"issue":"2","author":[{"full_name":"Maiuri, Paolo","last_name":"Maiuri","first_name":"Paolo"},{"last_name":"Rupprecht","first_name":"Jean","full_name":"Rupprecht, Jean"},{"id":"355AA5A0-F248-11E8-B48F-1D18A9856A87","first_name":"Stefan","last_name":"Wieser","orcid":"0000-0002-2670-2217","full_name":"Wieser, Stefan"},{"id":"4D71A03A-F248-11E8-B48F-1D18A9856A87","full_name":"Ruprecht, Verena","orcid":"0000-0003-4088-8633","last_name":"Ruprecht","first_name":"Verena"},{"first_name":"Olivier","last_name":"Bénichou","full_name":"Bénichou, Olivier"},{"last_name":"Carpi","first_name":"Nicolas","full_name":"Carpi, Nicolas"},{"first_name":"Mathieu","last_name":"Coppey","full_name":"Coppey, Mathieu"},{"last_name":"De Beco","first_name":"Simon","full_name":"De Beco, Simon"},{"first_name":"Nir","last_name":"Gov","full_name":"Gov, Nir"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566"},{"first_name":"Carolina","last_name":"Lage Crespo","full_name":"Lage Crespo, Carolina"},{"last_name":"Lautenschlaeger","first_name":"Franziska","full_name":"Lautenschlaeger, Franziska"},{"full_name":"Le Berre, Maël","first_name":"Maël","last_name":"Le Berre"},{"full_name":"Lennon Duménil, Ana","last_name":"Lennon Duménil","first_name":"Ana"},{"full_name":"Raab, Matthew","first_name":"Matthew","last_name":"Raab"},{"full_name":"Thiam, Hawa","last_name":"Thiam","first_name":"Hawa"},{"first_name":"Matthieu","last_name":"Piel","full_name":"Piel, Matthieu"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179","last_name":"Sixt","first_name":"Michael K"},{"first_name":"Raphaël","last_name":"Voituriez","full_name":"Voituriez, Raphaël"}],"scopus_import":1,"publication":"Cell","_id":"1553","intvolume":"       161","title":"Actin flows mediate a universal coupling between cell speed and cell persistence","month":"04","date_created":"2018-12-11T11:52:41Z","department":[{"_id":"MiSi"},{"_id":"CaHe"}],"project":[{"_id":"2529486C-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","grant_number":"T 560-B17","name":"Cell- and Tissue Mechanics in Zebrafish Germ Layer Formation"},{"grant_number":"281556","name":"Cytoskeletal force generation and force transduction of migrating leukocytes (EU)","_id":"25A603A2-B435-11E9-9278-68D0E5697425","call_identifier":"FP7"},{"grant_number":"RGP0058/2011","name":"Cell migration in complex environments: from in vivo experiments to theoretical models","_id":"25ABD200-B435-11E9-9278-68D0E5697425"}],"oa_version":"None","publication_status":"published","language":[{"iso":"eng"}],"ec_funded":1,"quality_controlled":"1","page":"374 - 386","publisher":"Cell Press","type":"journal_article","date_published":"2015-04-09T00:00:00Z","year":"2015","citation":{"mla":"Maiuri, Paolo, et al. “Actin Flows Mediate a Universal Coupling between Cell Speed and Cell Persistence.” <i>Cell</i>, vol. 161, no. 2, Cell Press, 2015, pp. 374–86, doi:<a href=\"https://doi.org/10.1016/j.cell.2015.01.056\">10.1016/j.cell.2015.01.056</a>.","short":"P. Maiuri, J. Rupprecht, S. Wieser, V. Ruprecht, O. Bénichou, N. Carpi, M. Coppey, S. De Beco, N. Gov, C.-P.J. Heisenberg, C. Lage Crespo, F. Lautenschlaeger, M. Le Berre, A. Lennon Duménil, M. Raab, H. Thiam, M. Piel, M.K. Sixt, R. Voituriez, Cell 161 (2015) 374–386.","ista":"Maiuri P, Rupprecht J, Wieser S, Ruprecht V, Bénichou O, Carpi N, Coppey M, De Beco S, Gov N, Heisenberg C-PJ, Lage Crespo C, Lautenschlaeger F, Le Berre M, Lennon Duménil A, Raab M, Thiam H, Piel M, Sixt MK, Voituriez R. 2015. Actin flows mediate a universal coupling between cell speed and cell persistence. Cell. 161(2), 374–386.","ama":"Maiuri P, Rupprecht J, Wieser S, et al. Actin flows mediate a universal coupling between cell speed and cell persistence. <i>Cell</i>. 2015;161(2):374-386. doi:<a href=\"https://doi.org/10.1016/j.cell.2015.01.056\">10.1016/j.cell.2015.01.056</a>","apa":"Maiuri, P., Rupprecht, J., Wieser, S., Ruprecht, V., Bénichou, O., Carpi, N., … Voituriez, R. (2015). Actin flows mediate a universal coupling between cell speed and cell persistence. <i>Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cell.2015.01.056\">https://doi.org/10.1016/j.cell.2015.01.056</a>","ieee":"P. Maiuri <i>et al.</i>, “Actin flows mediate a universal coupling between cell speed and cell persistence,” <i>Cell</i>, vol. 161, no. 2. Cell Press, pp. 374–386, 2015.","chicago":"Maiuri, Paolo, Jean Rupprecht, Stefan Wieser, Verena Ruprecht, Olivier Bénichou, Nicolas Carpi, Mathieu Coppey, et al. “Actin Flows Mediate a Universal Coupling between Cell Speed and Cell Persistence.” <i>Cell</i>. Cell Press, 2015. <a href=\"https://doi.org/10.1016/j.cell.2015.01.056\">https://doi.org/10.1016/j.cell.2015.01.056</a>."},"date_updated":"2021-01-12T06:51:33Z","publist_id":"5618","abstract":[{"text":"Cell movement has essential functions in development, immunity, and cancer. Various cell migration patterns have been reported, but no general rule has emerged so far. Here, we show on the basis of experimental data in vitro and in vivo that cell persistence, which quantifies the straightness of trajectories, is robustly coupled to cell migration speed. We suggest that this universal coupling constitutes a generic law of cell migration, which originates in the advection of polarity cues by an actin cytoskeleton undergoing flows at the cellular scale. Our analysis relies on a theoretical model that we validate by measuring the persistence of cells upon modulation of actin flow speeds and upon optogenetic manipulation of the binding of an actin regulator to actin filaments. Beyond the quantitative prediction of the coupling, the model yields a generic phase diagram of cellular trajectories, which recapitulates the full range of observed migration patterns.","lang":"eng"}],"day":"09","doi":"10.1016/j.cell.2015.01.056","status":"public","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","volume":161},{"year":"2015","citation":{"mla":"Gómez Sicilia, Àngel, et al. “An Exploration of the Universe of Polyglutamine Structures.” <i>PLoS Computational Biology</i>, vol. 11, no. 10, e1004541, Public Library of Science, 2015, doi:<a href=\"https://doi.org/10.1371/journal.pcbi.1004541\">10.1371/journal.pcbi.1004541</a>.","short":"À. Gómez Sicilia, M.K. Sikora, M. Cieplak, M. Carrión Vázquez, PLoS Computational Biology 11 (2015).","ista":"Gómez Sicilia À, Sikora MK, Cieplak M, Carrión Vázquez M. 2015. An exploration of the universe of polyglutamine structures. PLoS Computational Biology. 11(10), e1004541.","apa":"Gómez Sicilia, À., Sikora, M. K., Cieplak, M., &#38; Carrión Vázquez, M. (2015). An exploration of the universe of polyglutamine structures. <i>PLoS Computational Biology</i>. Public Library of Science. <a href=\"https://doi.org/10.1371/journal.pcbi.1004541\">https://doi.org/10.1371/journal.pcbi.1004541</a>","ama":"Gómez Sicilia À, Sikora MK, Cieplak M, Carrión Vázquez M. An exploration of the universe of polyglutamine structures. <i>PLoS Computational Biology</i>. 2015;11(10). doi:<a href=\"https://doi.org/10.1371/journal.pcbi.1004541\">10.1371/journal.pcbi.1004541</a>","ieee":"À. Gómez Sicilia, M. K. Sikora, M. Cieplak, and M. Carrión Vázquez, “An exploration of the universe of polyglutamine structures,” <i>PLoS Computational Biology</i>, vol. 11, no. 10. Public Library of Science, 2015.","chicago":"Gómez Sicilia, Àngel, Mateusz K Sikora, Marek Cieplak, and Mariano Carrión Vázquez. “An Exploration of the Universe of Polyglutamine Structures.” <i>PLoS Computational Biology</i>. Public Library of Science, 2015. <a href=\"https://doi.org/10.1371/journal.pcbi.1004541\">https://doi.org/10.1371/journal.pcbi.1004541</a>."},"date_updated":"2023-02-23T14:05:55Z","abstract":[{"lang":"eng","text":"Deposits of misfolded proteins in the human brain are associated with the development of many neurodegenerative diseases. Recent studies show that these proteins have common traits even at the monomer level. Among them, a polyglutamine region that is present in huntingtin is known to exhibit a correlation between the length of the chain and the severity as well as the earliness of the onset of Huntington disease. Here, we apply bias exchange molecular dynamics to generate structures of polyglutamine expansions of several lengths and characterize the resulting independent conformations. We compare the properties of these conformations to those of the standard proteins, as well as to other homopolymeric tracts. We find that, similar to the previously studied polyvaline chains, the set of possible transient folds is much broader than the set of known-to-date folds, although the conformations have different structures. We show that the mechanical stability is not related to any simple geometrical characteristics of the structures. We demonstrate that long polyglutamine expansions result in higher mechanical stability than the shorter ones. They also have a longer life span and are substantially more prone to form knotted structures. The knotted region has an average length of 35 residues, similar to the typical threshold for most polyglutamine-related diseases. Similarly, changes in shape and mechanical stability appear once the total length of the peptide exceeds this threshold of 35 glutamine residues. We suggest that knotted conformers may also harm the cellular machinery and thus lead to disease."}],"day":"23","doi":"10.1371/journal.pcbi.1004541","ddc":["570"],"acknowledgement":"We acknowledge the support by the EU Joint Programme in Neurodegenerative Diseases (JPND AC14/00037) project. The project is supported through the following funding organisations under the aegis of JPND—www.jpnd.eu: Ireland, HRB; Poland, National Science Centre; and Spain, ISCIII. ","volume":11,"issue":"10","author":[{"last_name":"Gómez Sicilia","first_name":"Àngel","full_name":"Gómez Sicilia, Àngel"},{"last_name":"Sikora","first_name":"Mateusz K","full_name":"Sikora, Mateusz K","id":"2F74BCDE-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Cieplak, Marek","last_name":"Cieplak","first_name":"Marek"},{"full_name":"Carrión Vázquez, Mariano","last_name":"Carrión Vázquez","first_name":"Mariano"}],"scopus_import":1,"_id":"1566","intvolume":"        11","pubrep_id":"478","title":"An exploration of the universe of polyglutamine structures","date_created":"2018-12-11T11:52:45Z","department":[{"_id":"CaHe"}],"publication_status":"published","file_date_updated":"2020-07-14T12:45:02Z","quality_controlled":"1","publisher":"Public Library of Science","type":"journal_article","date_published":"2015-10-23T00:00:00Z","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"publist_id":"5605","oa":1,"status":"public","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","related_material":{"record":[{"status":"public","id":"9714","relation":"research_data"}]},"file":[{"access_level":"open_access","relation":"main_file","creator":"system","file_id":"5207","checksum":"8b67d729be663bfc9af04bfd94459655","file_size":1412511,"date_created":"2018-12-12T10:16:21Z","file_name":"IST-2016-478-v1+1_journal.pcbi.1004541.pdf","content_type":"application/pdf","date_updated":"2020-07-14T12:45:02Z"}],"has_accepted_license":"1","publication":"PLoS Computational Biology","article_number":"e1004541","month":"10","oa_version":"Published Version","language":[{"iso":"eng"}]},{"volume":161,"status":"public","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","doi":"10.1016/j.cell.2015.04.009","day":"23","abstract":[{"lang":"eng","text":"In animal embryos, morphogen gradients determine tissue patterning and morphogenesis. Shyer et al. provide evidence that, during vertebrate gut formation, tissue folding generates graded activity of signals required for subsequent steps of gut growth and differentiation, thereby revealing an intriguing link between tissue morphogenesis and morphogen gradient formation."}],"publist_id":"5590","date_updated":"2022-08-25T13:56:10Z","year":"2015","citation":{"ista":"Bollenbach MT, Heisenberg C-PJ. 2015. Gradients are shaping up. Cell. 161(3), 431–432.","short":"M.T. Bollenbach, C.-P.J. Heisenberg, Cell 161 (2015) 431–432.","mla":"Bollenbach, Mark Tobias, and Carl-Philipp J. Heisenberg. “Gradients Are Shaping Up.” <i>Cell</i>, vol. 161, no. 3, Cell Press, 2015, pp. 431–32, doi:<a href=\"https://doi.org/10.1016/j.cell.2015.04.009\">10.1016/j.cell.2015.04.009</a>.","ieee":"M. T. Bollenbach and C.-P. J. Heisenberg, “Gradients are shaping up,” <i>Cell</i>, vol. 161, no. 3. Cell Press, pp. 431–432, 2015.","chicago":"Bollenbach, Mark Tobias, and Carl-Philipp J Heisenberg. “Gradients Are Shaping Up.” <i>Cell</i>. Cell Press, 2015. <a href=\"https://doi.org/10.1016/j.cell.2015.04.009\">https://doi.org/10.1016/j.cell.2015.04.009</a>.","ama":"Bollenbach MT, Heisenberg C-PJ. Gradients are shaping up. <i>Cell</i>. 2015;161(3):431-432. doi:<a href=\"https://doi.org/10.1016/j.cell.2015.04.009\">10.1016/j.cell.2015.04.009</a>","apa":"Bollenbach, M. T., &#38; Heisenberg, C.-P. J. (2015). Gradients are shaping up. <i>Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cell.2015.04.009\">https://doi.org/10.1016/j.cell.2015.04.009</a>"},"date_published":"2015-04-23T00:00:00Z","type":"journal_article","publisher":"Cell Press","page":"431 - 432","quality_controlled":"1","language":[{"iso":"eng"}],"oa_version":"None","publication_status":"published","article_processing_charge":"No","date_created":"2018-12-11T11:52:50Z","department":[{"_id":"ToBo"},{"_id":"CaHe"}],"title":"Gradients are shaping up","month":"04","intvolume":"       161","_id":"1581","publication":"Cell","scopus_import":"1","author":[{"orcid":"0000-0003-4398-476X","full_name":"Bollenbach, Mark Tobias","first_name":"Mark Tobias","last_name":"Bollenbach","id":"3E6DB97A-F248-11E8-B48F-1D18A9856A87"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","last_name":"Heisenberg","first_name":"Carl-Philipp J"}],"issue":"3"},{"doi":"10.1371/journal.pcbi.1004541.s001","day":"23","date_published":"2015-10-23T00:00:00Z","type":"research_data_reference","date_updated":"2023-02-23T10:04:35Z","citation":{"short":"À. Gómez Sicilia, M.K. Sikora, M. Cieplak, M. Carrión Vázquez, (2015).","mla":"Gómez Sicilia, Àngel, et al. <i>An Exploration of the Universe of Polyglutamine Structures - Submission to PLOS Journals</i>. Public Library of Science , 2015, doi:<a href=\"https://doi.org/10.1371/journal.pcbi.1004541.s001\">10.1371/journal.pcbi.1004541.s001</a>.","ista":"Gómez Sicilia À, Sikora MK, Cieplak M, Carrión Vázquez M. 2015. An exploration of the universe of polyglutamine structures - submission to PLOS journals, Public Library of Science , <a href=\"https://doi.org/10.1371/journal.pcbi.1004541.s001\">10.1371/journal.pcbi.1004541.s001</a>.","apa":"Gómez Sicilia, À., Sikora, M. K., Cieplak, M., &#38; Carrión Vázquez, M. (2015). An exploration of the universe of polyglutamine structures - submission to PLOS journals. Public Library of Science . <a href=\"https://doi.org/10.1371/journal.pcbi.1004541.s001\">https://doi.org/10.1371/journal.pcbi.1004541.s001</a>","ama":"Gómez Sicilia À, Sikora MK, Cieplak M, Carrión Vázquez M. An exploration of the universe of polyglutamine structures - submission to PLOS journals. 2015. doi:<a href=\"https://doi.org/10.1371/journal.pcbi.1004541.s001\">10.1371/journal.pcbi.1004541.s001</a>","chicago":"Gómez Sicilia, Àngel, Mateusz K Sikora, Marek Cieplak, and Mariano Carrión Vázquez. “An Exploration of the Universe of Polyglutamine Structures - Submission to PLOS Journals.” Public Library of Science , 2015. <a href=\"https://doi.org/10.1371/journal.pcbi.1004541.s001\">https://doi.org/10.1371/journal.pcbi.1004541.s001</a>.","ieee":"À. Gómez Sicilia, M. K. Sikora, M. Cieplak, and M. Carrión Vázquez, “An exploration of the universe of polyglutamine structures - submission to PLOS journals.” Public Library of Science , 2015."},"year":"2015","user_id":"6785fbc1-c503-11eb-8a32-93094b40e1cf","status":"public","related_material":{"record":[{"id":"1566","relation":"used_in_publication","status":"public"}]},"title":"An exploration of the universe of polyglutamine structures - submission to PLOS journals","month":"10","oa_version":"Published Version","date_created":"2021-07-23T12:05:28Z","article_processing_charge":"No","department":[{"_id":"CaHe"}],"author":[{"full_name":"Gómez Sicilia, Àngel","first_name":"Àngel","last_name":"Gómez Sicilia"},{"id":"2F74BCDE-F248-11E8-B48F-1D18A9856A87","full_name":"Sikora, Mateusz K","last_name":"Sikora","first_name":"Mateusz K"},{"full_name":"Cieplak, Marek","last_name":"Cieplak","first_name":"Marek"},{"full_name":"Carrión Vázquez, Mariano","first_name":"Mariano","last_name":"Carrión Vázquez"}],"_id":"9714","publisher":"Public Library of Science "},{"language":[{"iso":"eng"}],"keyword":["Developmental Biology","Embryology","General Medicine","Pediatrics","Perinatology","and Child Health"],"publication":"Congenital Anomalies","month":"02","oa_version":"None","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","status":"public","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1111/cga.12039"}],"date_published":"2014-02-01T00:00:00Z","type":"journal_article","oa":1,"publication_identifier":{"issn":["0914-3505"]},"page":"1-7","quality_controlled":"1","article_type":"original","publisher":"Wiley","author":[{"last_name":"Hashimoto","first_name":"Masakazu","full_name":"Hashimoto, Masakazu"},{"id":"4C6E54C6-F248-11E8-B48F-1D18A9856A87","first_name":"Hitoshi","last_name":"Morita","full_name":"Morita, Hitoshi"},{"last_name":"Ueno","first_name":"Naoto","full_name":"Ueno, Naoto"}],"issue":"1","pmid":1,"_id":"10815","scopus_import":"1","title":"Molecular and cellular mechanisms of development underlying congenital diseases","intvolume":"        54","publication_status":"published","department":[{"_id":"CaHe"}],"date_created":"2022-03-04T08:17:25Z","article_processing_charge":"No","acknowledgement":"The authors thank all the members of the Division of Morphogenesis, National Institute for Basic Biology, for their contributions to the research, their encouragement, and helpful discussions, particularly Dr M. Suzuki for his critical reading of the manuscript. We also thank the Model Animal Research and Spectrography and Bioimaging Facilities, NIBB Core Research Facilities, for technical support. M.H. was supported by a research fellowship from the Japan Society for the Promotion of Science (JSPS). Our work introduced in this review was supported by a Grant-in-Aid for Scientific Research on Innovative Areas from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT), Japan, to N.U.","volume":54,"external_id":{"pmid":["24666178"]},"date_updated":"2022-03-04T08:26:05Z","year":"2014","citation":{"ista":"Hashimoto M, Morita H, Ueno N. 2014. Molecular and cellular mechanisms of development underlying congenital diseases. Congenital Anomalies. 54(1), 1–7.","mla":"Hashimoto, Masakazu, et al. “Molecular and Cellular Mechanisms of Development Underlying Congenital Diseases.” <i>Congenital Anomalies</i>, vol. 54, no. 1, Wiley, 2014, pp. 1–7, doi:<a href=\"https://doi.org/10.1111/cga.12039\">10.1111/cga.12039</a>.","short":"M. Hashimoto, H. Morita, N. Ueno, Congenital Anomalies 54 (2014) 1–7.","ieee":"M. Hashimoto, H. Morita, and N. Ueno, “Molecular and cellular mechanisms of development underlying congenital diseases,” <i>Congenital Anomalies</i>, vol. 54, no. 1. Wiley, pp. 1–7, 2014.","chicago":"Hashimoto, Masakazu, Hitoshi Morita, and Naoto Ueno. “Molecular and Cellular Mechanisms of Development Underlying Congenital Diseases.” <i>Congenital Anomalies</i>. Wiley, 2014. <a href=\"https://doi.org/10.1111/cga.12039\">https://doi.org/10.1111/cga.12039</a>.","apa":"Hashimoto, M., Morita, H., &#38; Ueno, N. (2014). Molecular and cellular mechanisms of development underlying congenital diseases. <i>Congenital Anomalies</i>. Wiley. <a href=\"https://doi.org/10.1111/cga.12039\">https://doi.org/10.1111/cga.12039</a>","ama":"Hashimoto M, Morita H, Ueno N. Molecular and cellular mechanisms of development underlying congenital diseases. <i>Congenital Anomalies</i>. 2014;54(1):1-7. doi:<a href=\"https://doi.org/10.1111/cga.12039\">10.1111/cga.12039</a>"},"abstract":[{"lang":"eng","text":"In the last several decades, developmental biology has clarified the molecular mechanisms of embryogenesis and organogenesis. In particular, it has demonstrated that the “tool-kit genes” essential for regulating developmental processes are not only highly conserved among species, but are also used as systems at various times and places in an organism to control distinct developmental events. Therefore, mutations in many of these tool-kit genes may cause congenital diseases involving morphological abnormalities. This link between genes and abnormal morphological phenotypes underscores the importance of understanding how cells behave and contribute to morphogenesis as a result of gene function. Recent improvements in live imaging and in quantitative analyses of cellular dynamics will advance our understanding of the cellular pathogenesis of congenital diseases associated with aberrant morphologies. In these studies, it is critical to select an appropriate model organism for the particular phenomenon of interest."}],"doi":"10.1111/cga.12039","day":"01"},{"title":"Theoretical tests of the mechanical protection strategy in protein nanomechanics","month":"05","intvolume":"        82","oa_version":"None","publication_status":"published","department":[{"_id":"CaHe"}],"date_created":"2018-12-11T11:54:34Z","author":[{"full_name":"Chwastyk, Mateusz","last_name":"Chwastyk","first_name":"Mateusz"},{"first_name":"Albert","last_name":"Galera Prat","full_name":"Galera Prat, Albert"},{"full_name":"Sikora, Mateusz K","last_name":"Sikora","first_name":"Mateusz K","id":"2F74BCDE-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Gómez Sicilia, Àngel","last_name":"Gómez Sicilia","first_name":"Àngel"},{"first_name":"Mariano","last_name":"Carrión Vázquez","full_name":"Carrión Vázquez, Mariano"},{"last_name":"Cieplak","first_name":"Marek","full_name":"Cieplak, Marek"}],"issue":"5","publication":"Proteins: Structure, Function and Bioinformatics","_id":"1891","scopus_import":1,"publisher":"Wiley-Blackwell","language":[{"iso":"eng"}],"page":"717 - 726","abstract":[{"lang":"eng","text":"We provide theoretical tests of a novel experimental technique to determine mechanostability of proteins based on stretching a mechanically protected protein by single-molecule force spectroscopy. This technique involves stretching a homogeneous or heterogeneous chain of reference proteins (single-molecule markers) in which one of them acts as host to the guest protein under study. The guest protein is grafted into the host through genetic engineering. It is expected that unraveling of the host precedes the unraveling of the guest removing ambiguities in the reading of the force-extension patterns of the guest protein. We study examples of such systems within a coarse-grained structure-based model. We consider systems with various ratios of mechanostability for the host and guest molecules and compare them to experimental results involving cohesin I as the guest molecule. For a comparison, we also study the force-displacement patterns in proteins that are linked in a serial fashion. We find that the mechanostability of the guest is similar to that of the isolated or serially linked protein. We also demonstrate that the ideal configuration of this strategy would be one in which the host is much more mechanostable than the single-molecule markers. We finally show that it is troublesome to use the highly stable cystine knot proteins as a host to graft a guest in stretching studies because this would involve a cleaving procedure."}],"publist_id":"5204","doi":"10.1002/prot.24436","day":"01","date_published":"2014-05-01T00:00:00Z","type":"journal_article","date_updated":"2021-01-12T06:53:52Z","year":"2014","citation":{"ista":"Chwastyk M, Galera Prat A, Sikora MK, Gómez Sicilia À, Carrión Vázquez M, Cieplak M. 2014. Theoretical tests of the mechanical protection strategy in protein nanomechanics. Proteins: Structure, Function and Bioinformatics. 82(5), 717–726.","mla":"Chwastyk, Mateusz, et al. “Theoretical Tests of the Mechanical Protection Strategy in Protein Nanomechanics.” <i>Proteins: Structure, Function and Bioinformatics</i>, vol. 82, no. 5, Wiley-Blackwell, 2014, pp. 717–26, doi:<a href=\"https://doi.org/10.1002/prot.24436\">10.1002/prot.24436</a>.","short":"M. Chwastyk, A. Galera Prat, M.K. Sikora, À. Gómez Sicilia, M. Carrión Vázquez, M. Cieplak, Proteins: Structure, Function and Bioinformatics 82 (2014) 717–726.","chicago":"Chwastyk, Mateusz, Albert Galera Prat, Mateusz K Sikora, Àngel Gómez Sicilia, Mariano Carrión Vázquez, and Marek Cieplak. “Theoretical Tests of the Mechanical Protection Strategy in Protein Nanomechanics.” <i>Proteins: Structure, Function and Bioinformatics</i>. Wiley-Blackwell, 2014. <a href=\"https://doi.org/10.1002/prot.24436\">https://doi.org/10.1002/prot.24436</a>.","ieee":"M. Chwastyk, A. Galera Prat, M. K. Sikora, À. Gómez Sicilia, M. Carrión Vázquez, and M. Cieplak, “Theoretical tests of the mechanical protection strategy in protein nanomechanics,” <i>Proteins: Structure, Function and Bioinformatics</i>, vol. 82, no. 5. Wiley-Blackwell, pp. 717–726, 2014.","ama":"Chwastyk M, Galera Prat A, Sikora MK, Gómez Sicilia À, Carrión Vázquez M, Cieplak M. Theoretical tests of the mechanical protection strategy in protein nanomechanics. <i>Proteins: Structure, Function and Bioinformatics</i>. 2014;82(5):717-726. doi:<a href=\"https://doi.org/10.1002/prot.24436\">10.1002/prot.24436</a>","apa":"Chwastyk, M., Galera Prat, A., Sikora, M. K., Gómez Sicilia, À., Carrión Vázquez, M., &#38; Cieplak, M. (2014). Theoretical tests of the mechanical protection strategy in protein nanomechanics. <i>Proteins: Structure, Function and Bioinformatics</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1002/prot.24436\">https://doi.org/10.1002/prot.24436</a>"},"status":"public","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","volume":82,"acknowledgement":"Grant Nr. 2011/01/N/ST3/02475"},{"day":"31","doi":"10.1038/ncb2913","publist_id":"5195","abstract":[{"text":"Epithelial cell layers need to be tightly regulated to maintain their integrity and correct function. Cell integration into epithelial sheets is now shown to depend on the N-WASP-regulated stabilization of cortical F-actin, which generates distinct patterns of apical-lateral contractility at E-cadherin-based cell-cell junctions.","lang":"eng"}],"year":"2014","citation":{"mla":"Behrndt, Martin, and Carl-Philipp J. Heisenberg. “Lateral Junction Dynamics Lead the Way Out.” <i>Nature Cell Biology</i>, vol. 16, no. 2, Nature Publishing Group, 2014, pp. 127–29, doi:<a href=\"https://doi.org/10.1038/ncb2913\">10.1038/ncb2913</a>.","short":"M. Behrndt, C.-P.J. Heisenberg, Nature Cell Biology 16 (2014) 127–129.","ista":"Behrndt M, Heisenberg C-PJ. 2014. Lateral junction dynamics lead the way out. Nature Cell Biology. 16(2), 127–129.","apa":"Behrndt, M., &#38; Heisenberg, C.-P. J. (2014). Lateral junction dynamics lead the way out. <i>Nature Cell Biology</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/ncb2913\">https://doi.org/10.1038/ncb2913</a>","ama":"Behrndt M, Heisenberg C-PJ. Lateral junction dynamics lead the way out. <i>Nature Cell Biology</i>. 2014;16(2):127-129. doi:<a href=\"https://doi.org/10.1038/ncb2913\">10.1038/ncb2913</a>","chicago":"Behrndt, Martin, and Carl-Philipp J Heisenberg. “Lateral Junction Dynamics Lead the Way Out.” <i>Nature Cell Biology</i>. Nature Publishing Group, 2014. <a href=\"https://doi.org/10.1038/ncb2913\">https://doi.org/10.1038/ncb2913</a>.","ieee":"M. Behrndt and C.-P. J. Heisenberg, “Lateral junction dynamics lead the way out,” <i>Nature Cell Biology</i>, vol. 16, no. 2. Nature Publishing Group, pp. 127–129, 2014."},"date_updated":"2021-01-12T06:53:56Z","type":"journal_article","date_published":"2014-01-31T00:00:00Z","volume":16,"status":"public","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"CaHe"}],"date_created":"2018-12-11T11:54:37Z","publication_status":"published","oa_version":"None","intvolume":"        16","month":"01","title":"Lateral junction dynamics lead the way out","scopus_import":1,"_id":"1900","publication":"Nature Cell Biology","issue":"2","author":[{"full_name":"Behrndt, Martin","first_name":"Martin","last_name":"Behrndt","id":"3ECECA3A-F248-11E8-B48F-1D18A9856A87"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","last_name":"Heisenberg"}],"publisher":"Nature Publishing Group","quality_controlled":"1","page":"127 - 129","language":[{"iso":"eng"}]},{"day":"22","doi":"10.1016/j.devcel.2014.11.003","abstract":[{"text":"Kupffer's vesicle (KV) is the zebrafish organ of laterality, patterning the embryo along its left-right (LR) axis. Regional differences in cell shape within the lumen-lining KV epithelium are essential for its LR patterning function. However, the processes by which KV cells acquire their characteristic shapes are largely unknown. Here, we show that the notochord induces regional differences in cell shape within KV by triggering extracellular matrix (ECM) accumulation adjacent to anterior-dorsal (AD) regions of KV. This localized ECM deposition restricts apical expansion of lumen-lining epithelial cells in AD regions of KV during lumen growth. Our study provides mechanistic insight into the processes by which KV translates global embryonic patterning into regional cell shape differences required for its LR symmetry-breaking function.","lang":"eng"}],"citation":{"ama":"Compagnon J, Barone V, Rajshekar S, et al. The notochord breaks bilateral symmetry by controlling cell shapes in the Zebrafish laterality organ. <i>Developmental Cell</i>. 2014;31(6):774-783. doi:<a href=\"https://doi.org/10.1016/j.devcel.2014.11.003\">10.1016/j.devcel.2014.11.003</a>","apa":"Compagnon, J., Barone, V., Rajshekar, S., Kottmeier, R., Pranjic-Ferscha, K., Behrndt, M., &#38; Heisenberg, C.-P. J. (2014). The notochord breaks bilateral symmetry by controlling cell shapes in the Zebrafish laterality organ. <i>Developmental Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.devcel.2014.11.003\">https://doi.org/10.1016/j.devcel.2014.11.003</a>","chicago":"Compagnon, Julien, Vanessa Barone, Srivarsha Rajshekar, Rita Kottmeier, Kornelija Pranjic-Ferscha, Martin Behrndt, and Carl-Philipp J Heisenberg. “The Notochord Breaks Bilateral Symmetry by Controlling Cell Shapes in the Zebrafish Laterality Organ.” <i>Developmental Cell</i>. Cell Press, 2014. <a href=\"https://doi.org/10.1016/j.devcel.2014.11.003\">https://doi.org/10.1016/j.devcel.2014.11.003</a>.","ieee":"J. Compagnon <i>et al.</i>, “The notochord breaks bilateral symmetry by controlling cell shapes in the Zebrafish laterality organ,” <i>Developmental Cell</i>, vol. 31, no. 6. Cell Press, pp. 774–783, 2014.","mla":"Compagnon, Julien, et al. “The Notochord Breaks Bilateral Symmetry by Controlling Cell Shapes in the Zebrafish Laterality Organ.” <i>Developmental Cell</i>, vol. 31, no. 6, Cell Press, 2014, pp. 774–83, doi:<a href=\"https://doi.org/10.1016/j.devcel.2014.11.003\">10.1016/j.devcel.2014.11.003</a>.","short":"J. Compagnon, V. Barone, S. Rajshekar, R. Kottmeier, K. Pranjic-Ferscha, M. Behrndt, C.-P.J. Heisenberg, Developmental Cell 31 (2014) 774–783.","ista":"Compagnon J, Barone V, Rajshekar S, Kottmeier R, Pranjic-Ferscha K, Behrndt M, Heisenberg C-PJ. 2014. The notochord breaks bilateral symmetry by controlling cell shapes in the Zebrafish laterality organ. Developmental Cell. 31(6), 774–783."},"year":"2014","date_updated":"2023-09-07T12:05:08Z","external_id":{"pmid":["25535919"]},"volume":31,"acknowledgement":"We are grateful to members of the C.-P.H. lab, M. Concha, D. Siekhaus, and J. Vermot for comments on the manuscript and to M. Furutani-Seiki for sharing reagents. This work was supported by the Institute of Science and Technology Austria and an Alexander von Humboldt Foundation fellowship to J.C.","article_processing_charge":"No","department":[{"_id":"CaHe"}],"date_created":"2018-12-11T11:54:41Z","publication_status":"published","intvolume":"        31","title":"The notochord breaks bilateral symmetry by controlling cell shapes in the Zebrafish laterality organ","scopus_import":"1","_id":"1912","pmid":1,"issue":"6","author":[{"last_name":"Compagnon","first_name":"Julien","full_name":"Compagnon, Julien","id":"2E3E0988-F248-11E8-B48F-1D18A9856A87"},{"id":"419EECCC-F248-11E8-B48F-1D18A9856A87","full_name":"Barone, Vanessa","orcid":"0000-0003-2676-3367","last_name":"Barone","first_name":"Vanessa"},{"first_name":"Srivarsha","last_name":"Rajshekar","full_name":"Rajshekar, Srivarsha"},{"full_name":"Kottmeier, Rita","first_name":"Rita","last_name":"Kottmeier"},{"id":"4362B3C2-F248-11E8-B48F-1D18A9856A87","first_name":"Kornelija","last_name":"Pranjic-Ferscha","full_name":"Pranjic-Ferscha, Kornelija"},{"id":"3ECECA3A-F248-11E8-B48F-1D18A9856A87","full_name":"Behrndt, Martin","first_name":"Martin","last_name":"Behrndt"},{"last_name":"Heisenberg","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87"}],"publisher":"Cell Press","quality_controlled":"1","page":"774 - 783","oa":1,"publist_id":"5182","type":"journal_article","date_published":"2014-12-22T00:00:00Z","main_file_link":[{"url":"https://www.ncbi.nlm.nih.gov/pubmed/25535919","open_access":"1"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","status":"public","related_material":{"record":[{"status":"public","id":"961","relation":"dissertation_contains"}]},"oa_version":"Published Version","month":"12","publication":"Developmental Cell","language":[{"iso":"eng"}]},{"month":"06","article_number":"065005","oa_version":"Published Version","publication":"New Journal of Physics","has_accepted_license":"1","language":[{"iso":"eng"}],"publist_id":"5171","oa":1,"date_published":"2014-06-01T00:00:00Z","type":"journal_article","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"status":"public","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","file":[{"file_id":"5202","creator":"system","access_level":"open_access","relation":"main_file","date_updated":"2020-07-14T12:45:21Z","file_name":"IST-2016-429-v1+1_document.pdf","content_type":"application/pdf","date_created":"2018-12-12T10:16:16Z","checksum":"8dbe81ec656bf1264d8889bda9b2b985","file_size":941387}],"title":"Active elastic thin shell theory for cellular deformations","pubrep_id":"429","intvolume":"        16","publication_status":"published","date_created":"2018-12-11T11:54:44Z","department":[{"_id":"CaHe"}],"author":[{"last_name":"Berthoumieux","first_name":"Hélène","full_name":"Berthoumieux, Hélène"},{"id":"48F1E0D8-F248-11E8-B48F-1D18A9856A87","full_name":"Maître, Jean-Léon","orcid":"0000-0002-3688-1474","last_name":"Maître","first_name":"Jean-Léon"},{"full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","last_name":"Heisenberg","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Paluch, Ewa","first_name":"Ewa","last_name":"Paluch"},{"first_name":"Frank","last_name":"Julicher","full_name":"Julicher, Frank"},{"first_name":"Guillaume","last_name":"Salbreux","full_name":"Salbreux, Guillaume"}],"_id":"1923","scopus_import":1,"publisher":"IOP Publishing Ltd.","file_date_updated":"2020-07-14T12:45:21Z","quality_controlled":"1","abstract":[{"text":"We derive the equations for a thin, axisymmetric elastic shell subjected to an internal active stress giving rise to active tension and moments within the shell. We discuss the stability of a cylindrical elastic shell and its response to a localized change in internal active stress. This description is relevant to describe the cellular actomyosin cortex, a thin shell at the cell surface behaving elastically at a short timescale and subjected to active internal forces arising from myosin molecular motor activity. We show that the recent observations of cell deformation following detachment of adherent cells (Maître J-L et al 2012 Science 338 253-6) are well accounted for by this mechanical description. The actin cortex elastic and bending moduli can be obtained from a quantitative analysis of cell shapes observed in these experiments. Our approach thus provides a non-invasive, imaging-based method for the extraction of cellular physical parameters.","lang":"eng"}],"doi":"10.1088/1367-2630/16/6/065005","day":"01","date_updated":"2021-01-12T06:54:06Z","citation":{"chicago":"Berthoumieux, Hélène, Jean-Léon Maître, Carl-Philipp J Heisenberg, Ewa Paluch, Frank Julicher, and Guillaume Salbreux. “Active Elastic Thin Shell Theory for Cellular Deformations.” <i>New Journal of Physics</i>. IOP Publishing Ltd., 2014. <a href=\"https://doi.org/10.1088/1367-2630/16/6/065005\">https://doi.org/10.1088/1367-2630/16/6/065005</a>.","ieee":"H. Berthoumieux, J.-L. Maître, C.-P. J. Heisenberg, E. Paluch, F. Julicher, and G. Salbreux, “Active elastic thin shell theory for cellular deformations,” <i>New Journal of Physics</i>, vol. 16. IOP Publishing Ltd., 2014.","ama":"Berthoumieux H, Maître J-L, Heisenberg C-PJ, Paluch E, Julicher F, Salbreux G. Active elastic thin shell theory for cellular deformations. <i>New Journal of Physics</i>. 2014;16. doi:<a href=\"https://doi.org/10.1088/1367-2630/16/6/065005\">10.1088/1367-2630/16/6/065005</a>","apa":"Berthoumieux, H., Maître, J.-L., Heisenberg, C.-P. J., Paluch, E., Julicher, F., &#38; Salbreux, G. (2014). Active elastic thin shell theory for cellular deformations. <i>New Journal of Physics</i>. IOP Publishing Ltd. <a href=\"https://doi.org/10.1088/1367-2630/16/6/065005\">https://doi.org/10.1088/1367-2630/16/6/065005</a>","ista":"Berthoumieux H, Maître J-L, Heisenberg C-PJ, Paluch E, Julicher F, Salbreux G. 2014. Active elastic thin shell theory for cellular deformations. New Journal of Physics. 16, 065005.","mla":"Berthoumieux, Hélène, et al. “Active Elastic Thin Shell Theory for Cellular Deformations.” <i>New Journal of Physics</i>, vol. 16, 065005, IOP Publishing Ltd., 2014, doi:<a href=\"https://doi.org/10.1088/1367-2630/16/6/065005\">10.1088/1367-2630/16/6/065005</a>.","short":"H. Berthoumieux, J.-L. Maître, C.-P.J. Heisenberg, E. Paluch, F. Julicher, G. Salbreux, New Journal of Physics 16 (2014)."},"year":"2014","ddc":["570"],"volume":16},{"publisher":"IOP Publishing","article_type":"original","file_date_updated":"2020-07-14T12:45:21Z","publication_status":"published","article_processing_charge":"No","department":[{"_id":"CaHe"},{"_id":"MiSi"}],"date_created":"2018-12-11T11:54:45Z","title":"A single-molecule approach to explore binding uptake and transport of cancer cell targeting nanotubes","intvolume":"        25","_id":"1925","scopus_import":1,"author":[{"last_name":"Lamprecht","first_name":"Constanze","full_name":"Lamprecht, Constanze"},{"last_name":"Plochberger","first_name":"Birgit","full_name":"Plochberger, Birgit"},{"id":"4D71A03A-F248-11E8-B48F-1D18A9856A87","first_name":"Verena","last_name":"Ruprecht","orcid":"0000-0003-4088-8633","full_name":"Ruprecht, Verena"},{"full_name":"Wieser, Stefan","orcid":"0000-0002-2670-2217","last_name":"Wieser","first_name":"Stefan","id":"355AA5A0-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Rankl","first_name":"Christian","full_name":"Rankl, Christian"},{"first_name":"Elena","last_name":"Heister","full_name":"Heister, Elena"},{"full_name":"Unterauer, Barbara","last_name":"Unterauer","first_name":"Barbara"},{"full_name":"Brameshuber, Mario","first_name":"Mario","last_name":"Brameshuber"},{"first_name":"Jürgen","last_name":"Danzberger","full_name":"Danzberger, Jürgen"},{"full_name":"Lukanov, Petar","last_name":"Lukanov","first_name":"Petar"},{"first_name":"Emmanuel","last_name":"Flahaut","full_name":"Flahaut, Emmanuel"},{"first_name":"Gerhard","last_name":"Schütz","full_name":"Schütz, Gerhard"},{"full_name":"Hinterdorfer, Peter","first_name":"Peter","last_name":"Hinterdorfer"},{"full_name":"Ebner, Andreas","last_name":"Ebner","first_name":"Andreas"}],"issue":"12","acknowledgement":"This work was supported by EC grant Marie Curie RTN-CT-2006-035616, CARBIO 'Carbon nanotubes for biomedical applications' and Austrian FFG grant mnt-era.net 823980, 'IntelliTip'.\r\n","volume":25,"ddc":["570"],"doi":"10.1088/0957-4484/25/12/125704","day":"28","abstract":[{"text":"In the past decade carbon nanotubes (CNTs) have been widely studied as a potential drug-delivery system, especially with functionality for cellular targeting. Yet, little is known about the actual process of docking to cell receptors and transport dynamics after internalization. Here we performed single-particle studies of folic acid (FA) mediated CNT binding to human carcinoma cells and their transport inside the cytosol. In particular, we employed molecular recognition force spectroscopy, an atomic force microscopy based method, to visualize and quantify docking of FA functionalized CNTs to FA binding receptors in terms of binding probability and binding force. We then traced individual fluorescently labeled, FA functionalized CNTs after specific uptake, and created a dynamic 'roadmap' that clearly showed trajectories of directed diffusion and areas of nanotube confinement in the cytosol. Our results demonstrate the potential of a single-molecule approach for investigation of drug-delivery vehicles and their targeting capacity.","lang":"eng"}],"date_updated":"2021-01-12T06:54:07Z","citation":{"apa":"Lamprecht, C., Plochberger, B., Ruprecht, V., Wieser, S., Rankl, C., Heister, E., … Ebner, A. (2014). A single-molecule approach to explore binding uptake and transport of cancer cell targeting nanotubes. <i>Nanotechnology</i>. IOP Publishing. <a href=\"https://doi.org/10.1088/0957-4484/25/12/125704\">https://doi.org/10.1088/0957-4484/25/12/125704</a>","ama":"Lamprecht C, Plochberger B, Ruprecht V, et al. A single-molecule approach to explore binding uptake and transport of cancer cell targeting nanotubes. <i>Nanotechnology</i>. 2014;25(12). doi:<a href=\"https://doi.org/10.1088/0957-4484/25/12/125704\">10.1088/0957-4484/25/12/125704</a>","ieee":"C. Lamprecht <i>et al.</i>, “A single-molecule approach to explore binding uptake and transport of cancer cell targeting nanotubes,” <i>Nanotechnology</i>, vol. 25, no. 12. IOP Publishing, 2014.","chicago":"Lamprecht, Constanze, Birgit Plochberger, Verena Ruprecht, Stefan Wieser, Christian Rankl, Elena Heister, Barbara Unterauer, et al. “A Single-Molecule Approach to Explore Binding Uptake and Transport of Cancer Cell Targeting Nanotubes.” <i>Nanotechnology</i>. IOP Publishing, 2014. <a href=\"https://doi.org/10.1088/0957-4484/25/12/125704\">https://doi.org/10.1088/0957-4484/25/12/125704</a>.","short":"C. Lamprecht, B. Plochberger, V. Ruprecht, S. Wieser, C. Rankl, E. Heister, B. Unterauer, M. Brameshuber, J. Danzberger, P. Lukanov, E. Flahaut, G. Schütz, P. Hinterdorfer, A. Ebner, Nanotechnology 25 (2014).","mla":"Lamprecht, Constanze, et al. “A Single-Molecule Approach to Explore Binding Uptake and Transport of Cancer Cell Targeting Nanotubes.” <i>Nanotechnology</i>, vol. 25, no. 12, 125704, IOP Publishing, 2014, doi:<a href=\"https://doi.org/10.1088/0957-4484/25/12/125704\">10.1088/0957-4484/25/12/125704</a>.","ista":"Lamprecht C, Plochberger B, Ruprecht V, Wieser S, Rankl C, Heister E, Unterauer B, Brameshuber M, Danzberger J, Lukanov P, Flahaut E, Schütz G, Hinterdorfer P, Ebner A. 2014. A single-molecule approach to explore binding uptake and transport of cancer cell targeting nanotubes. Nanotechnology. 25(12), 125704."},"year":"2014","language":[{"iso":"eng"}],"oa_version":"Submitted Version","month":"03","article_number":"125704","publication":"Nanotechnology","has_accepted_license":"1","file":[{"file_id":"7856","creator":"dernst","access_level":"open_access","relation":"main_file","date_updated":"2020-07-14T12:45:21Z","content_type":"application/pdf","file_name":"2014_Nanotechnology_Lamprecht.pdf","date_created":"2020-05-15T09:21:19Z","checksum":"df4e03d225a19179e7790f6d87a12332","file_size":3804152}],"status":"public","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publist_id":"5169","oa":1,"date_published":"2014-03-28T00:00:00Z","type":"journal_article"},{"language":[{"iso":"eng"}],"quality_controlled":"1","page":"1 - 12","publisher":"Wiley-Blackwell","issue":"1","author":[{"id":"31C42484-F248-11E8-B48F-1D18A9856A87","full_name":"Capek, Daniel","orcid":"0000-0001-5199-9940","last_name":"Capek","first_name":"Daniel"},{"full_name":"Metscher, Brian","last_name":"Metscher","first_name":"Brian"},{"last_name":"Müller","first_name":"Gerd","full_name":"Müller, Gerd"}],"scopus_import":1,"_id":"2248","publication":"Journal of Experimental Zoology Part B: Molecular and Developmental Evolution","intvolume":"       322","month":"01","title":"Thumbs down: A molecular-morphogenetic approach to avian digit homology","date_created":"2018-12-11T11:56:33Z","department":[{"_id":"CaHe"}],"publication_status":"published","oa_version":"None","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","status":"public","volume":322,"type":"journal_article","date_published":"2014-01-01T00:00:00Z","citation":{"ama":"Capek D, Metscher B, Müller G. Thumbs down: A molecular-morphogenetic approach to avian digit homology. <i>Journal of Experimental Zoology Part B: Molecular and Developmental Evolution</i>. 2014;322(1):1-12. doi:<a href=\"https://doi.org/10.1002/jez.b.22545\">10.1002/jez.b.22545</a>","apa":"Capek, D., Metscher, B., &#38; Müller, G. (2014). Thumbs down: A molecular-morphogenetic approach to avian digit homology. <i>Journal of Experimental Zoology Part B: Molecular and Developmental Evolution</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1002/jez.b.22545\">https://doi.org/10.1002/jez.b.22545</a>","chicago":"Capek, Daniel, Brian Metscher, and Gerd Müller. “Thumbs down: A Molecular-Morphogenetic Approach to Avian Digit Homology.” <i>Journal of Experimental Zoology Part B: Molecular and Developmental Evolution</i>. Wiley-Blackwell, 2014. <a href=\"https://doi.org/10.1002/jez.b.22545\">https://doi.org/10.1002/jez.b.22545</a>.","ieee":"D. Capek, B. Metscher, and G. Müller, “Thumbs down: A molecular-morphogenetic approach to avian digit homology,” <i>Journal of Experimental Zoology Part B: Molecular and Developmental Evolution</i>, vol. 322, no. 1. Wiley-Blackwell, pp. 1–12, 2014.","short":"D. Capek, B. Metscher, G. Müller, Journal of Experimental Zoology Part B: Molecular and Developmental Evolution 322 (2014) 1–12.","mla":"Capek, Daniel, et al. “Thumbs down: A Molecular-Morphogenetic Approach to Avian Digit Homology.” <i>Journal of Experimental Zoology Part B: Molecular and Developmental Evolution</i>, vol. 322, no. 1, Wiley-Blackwell, 2014, pp. 1–12, doi:<a href=\"https://doi.org/10.1002/jez.b.22545\">10.1002/jez.b.22545</a>.","ista":"Capek D, Metscher B, Müller G. 2014. Thumbs down: A molecular-morphogenetic approach to avian digit homology. Journal of Experimental Zoology Part B: Molecular and Developmental Evolution. 322(1), 1–12."},"year":"2014","date_updated":"2021-01-12T06:56:16Z","publist_id":"4701","abstract":[{"lang":"eng","text":"Avian forelimb digit homology remains one of the standard themes in comparative biology and EvoDevo research. In order to resolve the apparent contradictions between embryological and paleontological evidence a variety of hypotheses have been presented in recent years. The proposals range from excluding birds from the dinosaur clade, to assignments of homology by different criteria, or even assuming a hexadactyl tetrapod limb ground state. At present two approaches prevail: the frame shift hypothesis and the pyramid reduction hypothesis. While the former postulates a homeotic shift of digit identities, the latter argues for a gradual bilateral reduction of phalanges and digits. Here we present a new model that integrates elements from both hypotheses with the existing experimental and fossil evidence. We start from the main feature common to both earlier concepts, the initiating ontogenetic event: reduction and loss of the anterior-most digit. It is proposed that a concerted mechanism of molecular regulation and developmental mechanics is capable of shifting the boundaries of hoxD expression in embryonic forelimb buds as well as changing the digit phenotypes. Based on a distinction between positional (topological) and compositional (phenotypic) homology criteria, we argue that the identity of the avian digits is II, III, IV, despite a partially altered phenotype. Finally, we introduce an alternative digit reduction scheme that reconciles the current fossil evidence with the presented molecular-morphogenetic model. Our approach identifies specific experiments that allow to test whether gene expression can be shifted and digit phenotypes can be altered by induced digit loss or digit gain."}],"publication_identifier":{"issn":["15525007"]},"day":"01","doi":"10.1002/jez.b.22545"},{"date_published":"2014-08-22T00:00:00Z","type":"book_chapter","publication_identifier":{"isbn":["9781493911639","9781493911646"],"eissn":["1940-6029"],"issn":["1064-3745"]},"place":"New York, NY","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","status":"public","publication":"Tissue Morphogenesis","oa_version":"None","month":"08","language":[{"iso":"eng"}],"date_updated":"2023-09-05T14:12:00Z","year":"2014","citation":{"chicago":"Smutny, Michael, Martin Behrndt, Pedro Campinho, Verena Ruprecht, and Carl-Philipp J Heisenberg. “UV Laser Ablation to Measure Cell and Tissue-Generated Forces in the Zebrafish Embryo in Vivo and Ex Vivo.” In <i>Tissue Morphogenesis</i>, edited by Celeste Nelson, 1189:219–35. Methods in Molecular Biology. New York, NY: Springer, 2014. <a href=\"https://doi.org/10.1007/978-1-4939-1164-6_15\">https://doi.org/10.1007/978-1-4939-1164-6_15</a>.","ieee":"M. Smutny, M. Behrndt, P. Campinho, V. Ruprecht, and C.-P. J. Heisenberg, “UV laser ablation to measure cell and tissue-generated forces in the zebrafish embryo in vivo and ex vivo,” in <i>Tissue Morphogenesis</i>, vol. 1189, C. Nelson, Ed. New York, NY: Springer, 2014, pp. 219–235.","apa":"Smutny, M., Behrndt, M., Campinho, P., Ruprecht, V., &#38; Heisenberg, C.-P. J. (2014). UV laser ablation to measure cell and tissue-generated forces in the zebrafish embryo in vivo and ex vivo. In C. Nelson (Ed.), <i>Tissue Morphogenesis</i> (Vol. 1189, pp. 219–235). New York, NY: Springer. <a href=\"https://doi.org/10.1007/978-1-4939-1164-6_15\">https://doi.org/10.1007/978-1-4939-1164-6_15</a>","ama":"Smutny M, Behrndt M, Campinho P, Ruprecht V, Heisenberg C-PJ. UV laser ablation to measure cell and tissue-generated forces in the zebrafish embryo in vivo and ex vivo. In: Nelson C, ed. <i>Tissue Morphogenesis</i>. Vol 1189. Methods in Molecular Biology. New York, NY: Springer; 2014:219-235. doi:<a href=\"https://doi.org/10.1007/978-1-4939-1164-6_15\">10.1007/978-1-4939-1164-6_15</a>","ista":"Smutny M, Behrndt M, Campinho P, Ruprecht V, Heisenberg C-PJ. 2014.UV laser ablation to measure cell and tissue-generated forces in the zebrafish embryo in vivo and ex vivo. In: Tissue Morphogenesis. vol. 1189, 219–235.","short":"M. Smutny, M. Behrndt, P. Campinho, V. Ruprecht, C.-P.J. Heisenberg, in:, C. Nelson (Ed.), Tissue Morphogenesis, Springer, New York, NY, 2014, pp. 219–235.","mla":"Smutny, Michael, et al. “UV Laser Ablation to Measure Cell and Tissue-Generated Forces in the Zebrafish Embryo in Vivo and Ex Vivo.” <i>Tissue Morphogenesis</i>, edited by Celeste Nelson, vol. 1189, Springer, 2014, pp. 219–35, doi:<a href=\"https://doi.org/10.1007/978-1-4939-1164-6_15\">10.1007/978-1-4939-1164-6_15</a>."},"external_id":{"pmid":["25245697"]},"doi":"10.1007/978-1-4939-1164-6_15","day":"22","abstract":[{"text":"Mechanically coupled cells can generate forces driving cell and tissue morphogenesis during development. Visualization and measuring of these forces is of major importance to better understand the complexity of the biomechanic processes that shape cells and tissues. Here, we describe how UV laser ablation can be utilized to quantitatively assess mechanical tension in different tissues of the developing zebrafish and in cultures of primary germ layer progenitor cells ex vivo.","lang":"eng"}],"volume":1189,"pmid":1,"_id":"6178","author":[{"id":"3FE6E4E8-F248-11E8-B48F-1D18A9856A87","first_name":"Michael","last_name":"Smutny","orcid":"0000-0002-5920-9090","full_name":"Smutny, Michael"},{"full_name":"Behrndt, Martin","last_name":"Behrndt","first_name":"Martin","id":"3ECECA3A-F248-11E8-B48F-1D18A9856A87"},{"id":"3AFBBC42-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8526-5416","full_name":"Campinho, Pedro","first_name":"Pedro","last_name":"Campinho"},{"id":"4D71A03A-F248-11E8-B48F-1D18A9856A87","full_name":"Ruprecht, Verena","orcid":"0000-0003-4088-8633","last_name":"Ruprecht","first_name":"Verena"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566"}],"publication_status":"published","date_created":"2019-03-26T08:55:59Z","article_processing_charge":"No","department":[{"_id":"CaHe"}],"title":"UV laser ablation to measure cell and tissue-generated forces in the zebrafish embryo in vivo and ex vivo","intvolume":"      1189","page":"219-235","quality_controlled":"1","series_title":"Methods in Molecular Biology","publisher":"Springer","editor":[{"first_name":"Celeste","last_name":"Nelson","full_name":"Nelson, Celeste"}]},{"language":[{"iso":"eng"}],"page":"91","publisher":"IST Austria","author":[{"id":"3ECECA3A-F248-11E8-B48F-1D18A9856A87","first_name":"Martin","last_name":"Behrndt","full_name":"Behrndt, Martin"}],"_id":"1403","alternative_title":["IST Austria Thesis"],"month":"08","title":"Forces driving epithelial spreading in zebrafish epiboly","oa_version":"None","acknowledged_ssus":[{"_id":"SSU"}],"publication_status":"published","department":[{"_id":"CaHe"}],"date_created":"2018-12-11T11:51:49Z","status":"public","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","related_material":{"record":[{"relation":"part_of_dissertation","id":"2282","status":"public"},{"status":"public","id":"2950","relation":"part_of_dissertation"},{"status":"public","relation":"part_of_dissertation","id":"3373"}]},"date_published":"2014-08-01T00:00:00Z","type":"dissertation","date_updated":"2023-10-17T12:16:58Z","year":"2014","citation":{"ama":"Behrndt M. Forces driving epithelial spreading in zebrafish epiboly. 2014.","apa":"Behrndt, M. (2014). <i>Forces driving epithelial spreading in zebrafish epiboly</i>. IST Austria.","chicago":"Behrndt, Martin. “Forces Driving Epithelial Spreading in Zebrafish Epiboly.” IST Austria, 2014.","ieee":"M. Behrndt, “Forces driving epithelial spreading in zebrafish epiboly,” IST Austria, 2014.","mla":"Behrndt, Martin. <i>Forces Driving Epithelial Spreading in Zebrafish Epiboly</i>. IST Austria, 2014.","short":"M. Behrndt, Forces Driving Epithelial Spreading in Zebrafish Epiboly, IST Austria, 2014.","ista":"Behrndt M. 2014. Forces driving epithelial spreading in zebrafish epiboly. IST Austria."},"abstract":[{"lang":"eng","text":"A variety of developmental and disease related processes depend on epithelial cell sheet spreading. In order to gain insight into the biophysical mechanism(s) underlying the tissue morphogenesis we studied the spreading of an epithelium during the early development of the zebrafish embryo. In zebrafish epiboly the enveloping cell layer (EVL), a simple squamous epithelium, spreads over the yolk cell to completely engulf it at the end of gastrulation. Previous studies have proposed that an actomyosin ring forming within the yolk syncytial layer (YSL) acts as purse string that through constriction along its circumference pulls on the margin of the EVL. Direct biophysical evidence for this hypothesis has however been missing. The aim of the thesis was to understand how the actomyosin ring may generate pulling forces onto the EVL and what cellular mechanism(s) may facilitate the spreading of the epithelium. Using laser ablation to measure cortical tension within the actomyosin ring we found an anisotropic tension distribution, which was highest along the circumference of the ring. However the low degree of anisotropy was incompatible with the actomyosin ring functioning as a purse string only. Additionally, we observed retrograde cortical flow from vegetal parts of the ring into the EVL margin. Interpreting the experimental data using a theoretical distribution that models  the tissues as active viscous gels led us to proposen that the actomyosin ring has a twofold contribution to EVL epiboly. It not only acts as a purse string through constriction along its circumference, but in addition constriction along the width of the ring generates pulling forces through friction-resisted cortical flow. Moreover, when rendering the purse string mechanism unproductive EVL epiboly proceeded normally indicating that the flow-friction mechanism is sufficient to drive the process. Aiming to understand what cellular mechanism(s) may facilitate the spreading of the epithelium we found that tension-oriented EVL cell divisions limit tissue anisotropy by releasing tension along the division axis and promote epithelial spreading. Notably, EVL cells undergo ectopic cell fusion in conditions in which oriented-cell division is impaired or the epithelium is mechanically challenged. Taken together our study of EVL epiboly suggests a novel mechanism of force generation for actomyosin rings through friction-resisted cortical flow and highlights the importance of tension-oriented cell divisions in epithelial morphogenesis."}],"supervisor":[{"id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566"}],"publist_id":"5804","day":"01"},{"ddc":["570"],"volume":23,"external_id":{"pmid":["23885883"]},"date_updated":"2021-01-12T06:57:40Z","year":"2013","citation":{"chicago":"Maître, Jean-Léon, and Carl-Philipp J Heisenberg. “Three Functions of Cadherins in Cell Adhesion.” <i>Current Biology</i>. Cell Press, 2013. <a href=\"https://doi.org/10.1016/j.cub.2013.06.019\">https://doi.org/10.1016/j.cub.2013.06.019</a>.","ieee":"J.-L. Maître and C.-P. J. Heisenberg, “Three functions of cadherins in cell adhesion,” <i>Current Biology</i>, vol. 23, no. 14. Cell Press, pp. R626–R633, 2013.","ama":"Maître J-L, Heisenberg C-PJ. Three functions of cadherins in cell adhesion. <i>Current Biology</i>. 2013;23(14):R626-R633. doi:<a href=\"https://doi.org/10.1016/j.cub.2013.06.019\">10.1016/j.cub.2013.06.019</a>","apa":"Maître, J.-L., &#38; Heisenberg, C.-P. J. (2013). Three functions of cadherins in cell adhesion. <i>Current Biology</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cub.2013.06.019\">https://doi.org/10.1016/j.cub.2013.06.019</a>","ista":"Maître J-L, Heisenberg C-PJ. 2013. Three functions of cadherins in cell adhesion. Current Biology. 23(14), R626–R633.","mla":"Maître, Jean-Léon, and Carl-Philipp J. Heisenberg. “Three Functions of Cadherins in Cell Adhesion.” <i>Current Biology</i>, vol. 23, no. 14, Cell Press, 2013, pp. R626–33, doi:<a href=\"https://doi.org/10.1016/j.cub.2013.06.019\">10.1016/j.cub.2013.06.019</a>.","short":"J.-L. Maître, C.-P.J. Heisenberg, Current Biology 23 (2013) R626–R633."},"abstract":[{"lang":"eng","text":"Cadherins are transmembrane proteins that mediate cell–cell adhesion in animals. By regulating contact formation and stability, cadherins play a crucial role in tissue morphogenesis and homeostasis. Here, we review the three major  unctions of cadherins in cell–cell contact formation and stability. Two of those functions lead to a decrease in interfacial ension at the forming cell–cell contact, thereby promoting contact expansion — first, by providing adhesion tension that lowers interfacial tension at the cell–cell contact, and second, by signaling to the actomyosin cytoskeleton in order to reduce cortex tension and thus interfacial tension at the contact. The third function of cadherins in cell–cell contact formation is to stabilize the contact by resisting mechanical forces that pull on the contact."}],"doi":"10.1016/j.cub.2013.06.019","day":"22","file_date_updated":"2020-07-14T12:45:41Z","page":"R626 - R633","quality_controlled":"1","publisher":"Cell Press","author":[{"last_name":"Maître","first_name":"Jean-Léon","full_name":"Maître, Jean-Léon","orcid":"0000-0002-3688-1474","id":"48F1E0D8-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87"}],"issue":"14","pmid":1,"_id":"2469","scopus_import":1,"title":"Three functions of cadherins in cell adhesion","intvolume":"        23","publication_status":"published","department":[{"_id":"CaHe"}],"date_created":"2018-12-11T11:57:51Z","status":"public","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","file":[{"content_type":"application/pdf","file_name":"2013_CurrentBiology_Maitre.pdf","date_updated":"2020-07-14T12:45:41Z","checksum":"6a424b2f007b41d4955a9135793b2162","file_size":247320,"date_created":"2019-01-24T15:40:22Z","creator":"dernst","file_id":"5881","access_level":"open_access","relation":"main_file"}],"date_published":"2013-07-22T00:00:00Z","type":"journal_article","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"publist_id":"4433","oa":1,"language":[{"iso":"eng"}],"publication":"Current Biology","has_accepted_license":"1","month":"07","oa_version":"Published Version"},{"publisher":"Cell Press","language":[{"iso":"eng"}],"quality_controlled":"1","page":"948 - 962","intvolume":"       153","month":"05","title":"Forces in tissue morphogenesis and patterning","date_created":"2018-12-11T11:59:50Z","department":[{"_id":"CaHe"}],"publication_status":"published","oa_version":"None","issue":"5","author":[{"id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","last_name":"Heisenberg"},{"first_name":"Yohanns","last_name":"Bellaïche","full_name":"Bellaïche, Yohanns"}],"scopus_import":1,"publication":"Cell","_id":"2833","status":"public","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","acknowledgement":"C.-P.H. is supported by the Institute of Science and Technology Austria and grants from the Deutsche Forschungsgemeinschaft (DFG) and Fonds zur Förderung der wissenschaftlichen Forschung (FWF).","volume":153,"publist_id":"3966","abstract":[{"text":"During development, mechanical forces cause changes in size, shape, number, position, and gene expression of cells. They are therefore integral to any morphogenetic processes. Force generation by actin-myosin networks and force transmission through adhesive complexes are two self-organizing phenomena driving tissue morphogenesis. Coordination and integration of forces by long-range force transmission and mechanosensing of cells within tissues produce large-scale tissue shape changes. Extrinsic mechanical forces also control tissue patterning by modulating cell fate specification and differentiation. Thus, the interplay between tissue mechanics and biochemical signaling orchestrates tissue morphogenesis and patterning in development.","lang":"eng"}],"day":"23","doi":"10.1016/j.cell.2013.05.008","type":"journal_article","date_published":"2013-05-23T00:00:00Z","year":"2013","citation":{"short":"C.-P.J. Heisenberg, Y. Bellaïche, Cell 153 (2013) 948–962.","mla":"Heisenberg, Carl-Philipp J., and Yohanns Bellaïche. “Forces in Tissue Morphogenesis and Patterning.” <i>Cell</i>, vol. 153, no. 5, Cell Press, 2013, pp. 948–62, doi:<a href=\"https://doi.org/10.1016/j.cell.2013.05.008\">10.1016/j.cell.2013.05.008</a>.","ista":"Heisenberg C-PJ, Bellaïche Y. 2013. Forces in tissue morphogenesis and patterning. Cell. 153(5), 948–962.","apa":"Heisenberg, C.-P. J., &#38; Bellaïche, Y. (2013). Forces in tissue morphogenesis and patterning. <i>Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cell.2013.05.008\">https://doi.org/10.1016/j.cell.2013.05.008</a>","ama":"Heisenberg C-PJ, Bellaïche Y. Forces in tissue morphogenesis and patterning. <i>Cell</i>. 2013;153(5):948-962. doi:<a href=\"https://doi.org/10.1016/j.cell.2013.05.008\">10.1016/j.cell.2013.05.008</a>","ieee":"C.-P. J. Heisenberg and Y. Bellaïche, “Forces in tissue morphogenesis and patterning,” <i>Cell</i>, vol. 153, no. 5. Cell Press, pp. 948–962, 2013.","chicago":"Heisenberg, Carl-Philipp J, and Yohanns Bellaïche. “Forces in Tissue Morphogenesis and Patterning.” <i>Cell</i>. Cell Press, 2013. <a href=\"https://doi.org/10.1016/j.cell.2013.05.008\">https://doi.org/10.1016/j.cell.2013.05.008</a>."},"date_updated":"2021-01-12T07:00:04Z"},{"scopus_import":1,"_id":"2841","publication":"Developmental Cell","issue":"6","author":[{"full_name":"Morita, Hitoshi","last_name":"Morita","first_name":"Hitoshi","id":"4C6E54C6-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","last_name":"Heisenberg","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87"}],"date_created":"2018-12-11T11:59:52Z","department":[{"_id":"CaHe"}],"publication_status":"published","oa_version":"None","intvolume":"        24","month":"05","title":"Holding on and letting go: Cadherin turnover in cell intercalation","quality_controlled":"1","page":"567 - 569","language":[{"iso":"eng"}],"publisher":"Cell Press","citation":{"apa":"Morita, H., &#38; Heisenberg, C.-P. J. (2013). Holding on and letting go: Cadherin turnover in cell intercalation. <i>Developmental Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.devcel.2013.03.007\">https://doi.org/10.1016/j.devcel.2013.03.007</a>","ama":"Morita H, Heisenberg C-PJ. Holding on and letting go: Cadherin turnover in cell intercalation. <i>Developmental Cell</i>. 2013;24(6):567-569. doi:<a href=\"https://doi.org/10.1016/j.devcel.2013.03.007\">10.1016/j.devcel.2013.03.007</a>","ieee":"H. Morita and C.-P. J. Heisenberg, “Holding on and letting go: Cadherin turnover in cell intercalation,” <i>Developmental Cell</i>, vol. 24, no. 6. Cell Press, pp. 567–569, 2013.","chicago":"Morita, Hitoshi, and Carl-Philipp J Heisenberg. “Holding on and Letting Go: Cadherin Turnover in Cell Intercalation.” <i>Developmental Cell</i>. Cell Press, 2013. <a href=\"https://doi.org/10.1016/j.devcel.2013.03.007\">https://doi.org/10.1016/j.devcel.2013.03.007</a>.","mla":"Morita, Hitoshi, and Carl-Philipp J. Heisenberg. “Holding on and Letting Go: Cadherin Turnover in Cell Intercalation.” <i>Developmental Cell</i>, vol. 24, no. 6, Cell Press, 2013, pp. 567–69, doi:<a href=\"https://doi.org/10.1016/j.devcel.2013.03.007\">10.1016/j.devcel.2013.03.007</a>.","short":"H. Morita, C.-P.J. Heisenberg, Developmental Cell 24 (2013) 567–569.","ista":"Morita H, Heisenberg C-PJ. 2013. Holding on and letting go: Cadherin turnover in cell intercalation. Developmental Cell. 24(6), 567–569."},"year":"2013","date_updated":"2021-01-12T07:00:09Z","type":"journal_article","date_published":"2013-05-25T00:00:00Z","day":"25","doi":"10.1016/j.devcel.2013.03.007","publist_id":"3956","abstract":[{"text":"In zebrafish early development, blastoderm cells undergo extensive radial intercalations, triggering the spreading of the blastoderm over the yolk cell and thereby initiating embryonic body axis formation. Now reporting in Developmental Cell, Song et al. (2013) demonstrate a critical function for EGF-dependent E-cadherin endocytosis in promoting blastoderm cell intercalations.","lang":"eng"}],"volume":24,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","status":"public"},{"abstract":[{"lang":"eng","text":"Motile cilia perform crucial functions during embryonic development and throughout adult life. Development of organs containing motile cilia involves regulation of cilia formation (ciliogenesis) and formation of a luminal space (lumenogenesis) in which cilia generate fluid flows. Control of ciliogenesis and lumenogenesis is not yet fully understood, and it remains unclear whether these processes are coupled. In the zebrafish embryo, lethal giant larvae 2 (lgl2) is expressed prominently in ciliated organs. Lgl proteins are involved in establishing cell polarity and have been implicated in vesicle trafficking. Here, we identified a role for Lgl2 in development of ciliated epithelia in Kupffer's vesicle, which directs left-right asymmetry of the embryo; the otic vesicles, which give rise to the inner ear; and the pronephric ducts of the kidney. Using Kupffer's vesicle as a model ciliated organ, we found that depletion of Lgl2 disrupted lumen formation and reduced cilia number and length. Immunofluorescence and time-lapse imaging of Kupffer's vesicle morphogenesis in Lgl2-deficient embryos suggested cell adhesion defects and revealed loss of the adherens junction component E-cadherin at lateral membranes. Genetic interaction experiments indicate that Lgl2 interacts with Rab11a to regulate E-cadherin and mediate lumen formation that is uncoupled from cilia formation. These results uncover new roles and interactions for Lgl2 that are crucial for both lumenogenesis and ciliogenesis and indicate that these processes are genetically separable in zebrafish."}],"day":"01","doi":"10.1242/dev.087130","external_id":{"pmid":["23482490"]},"citation":{"ama":"Tay H, Schulze S, Compagnon J, et al. Lethal giant larvae 2 regulates development of the ciliated organ Kupffer’s vesicle. <i>Development</i>. 2013;140(7):1550-1559. doi:<a href=\"https://doi.org/10.1242/dev.087130\">10.1242/dev.087130</a>","apa":"Tay, H., Schulze, S., Compagnon, J., Foley, F., Heisenberg, C.-P. J., Yost, H. J., … Amack, J. (2013). Lethal giant larvae 2 regulates development of the ciliated organ Kupffer’s vesicle. <i>Development</i>. Company of Biologists. <a href=\"https://doi.org/10.1242/dev.087130\">https://doi.org/10.1242/dev.087130</a>","chicago":"Tay, Hwee, Sabrina Schulze, Julien Compagnon, Fiona Foley, Carl-Philipp J Heisenberg, H Joseph Yost, Salim Abdelilah Seyfried, and Jeffrey Amack. “Lethal Giant Larvae 2 Regulates Development of the Ciliated Organ Kupffer’s Vesicle.” <i>Development</i>. Company of Biologists, 2013. <a href=\"https://doi.org/10.1242/dev.087130\">https://doi.org/10.1242/dev.087130</a>.","ieee":"H. Tay <i>et al.</i>, “Lethal giant larvae 2 regulates development of the ciliated organ Kupffer’s vesicle,” <i>Development</i>, vol. 140, no. 7. Company of Biologists, pp. 1550–1559, 2013.","mla":"Tay, Hwee, et al. “Lethal Giant Larvae 2 Regulates Development of the Ciliated Organ Kupffer’s Vesicle.” <i>Development</i>, vol. 140, no. 7, Company of Biologists, 2013, pp. 1550–59, doi:<a href=\"https://doi.org/10.1242/dev.087130\">10.1242/dev.087130</a>.","short":"H. Tay, S. Schulze, J. Compagnon, F. Foley, C.-P.J. Heisenberg, H.J. Yost, S. Abdelilah Seyfried, J. Amack, Development 140 (2013) 1550–1559.","ista":"Tay H, Schulze S, Compagnon J, Foley F, Heisenberg C-PJ, Yost HJ, Abdelilah Seyfried S, Amack J. 2013. Lethal giant larvae 2 regulates development of the ciliated organ Kupffer’s vesicle. Development. 140(7), 1550–1559."},"year":"2013","date_updated":"2021-01-12T07:00:20Z","acknowledgement":"Deposited in PMC for release after 12 months. We thank members of the Amack lab for helpful discussions and Mahendra Sonawane for donating reagents.","volume":140,"intvolume":"       140","title":"Lethal giant larvae 2 regulates development of the ciliated organ Kupffer’s vesicle","date_created":"2018-12-11T11:59:59Z","department":[{"_id":"CaHe"}],"publication_status":"published","issue":"7","author":[{"full_name":"Tay, Hwee","last_name":"Tay","first_name":"Hwee"},{"full_name":"Schulze, Sabrina","first_name":"Sabrina","last_name":"Schulze"},{"id":"2E3E0988-F248-11E8-B48F-1D18A9856A87","full_name":"Compagnon, Julien","last_name":"Compagnon","first_name":"Julien"},{"last_name":"Foley","first_name":"Fiona","full_name":"Foley, Fiona"},{"orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87"},{"first_name":"H Joseph","last_name":"Yost","full_name":"Yost, H Joseph"},{"first_name":"Salim","last_name":"Abdelilah Seyfried","full_name":"Abdelilah Seyfried, Salim"},{"first_name":"Jeffrey","last_name":"Amack","full_name":"Amack, Jeffrey"}],"scopus_import":1,"pmid":1,"_id":"2862","publisher":"Company of Biologists","quality_controlled":"1","page":"1550 - 1559","publist_id":"3927","oa":1,"type":"journal_article","date_published":"2013-04-01T00:00:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","status":"public","main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3596994/","open_access":"1"}],"month":"04","oa_version":"Submitted Version","publication":"Development","language":[{"iso":"eng"}]},{"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","status":"public","volume":29,"publist_id":"3877","doi":"10.1051/medsci/2013292011","day":"01","date_published":"2013-02-01T00:00:00Z","type":"journal_article","date_updated":"2021-01-12T07:00:28Z","year":"2013","citation":{"ista":"Maître J-L, Berthoumieux H, Krens G, Salbreux G, Julicher F, Paluch E, Heisenberg C-PJ. 2013. Cell adhesion mechanics of zebrafish gastrulation. Medecine Sciences. 29(2), 147–150.","mla":"Maître, Jean-Léon, et al. “Cell Adhesion Mechanics of Zebrafish Gastrulation.” <i>Medecine Sciences</i>, vol. 29, no. 2, Éditions Médicales et Scientifiques, 2013, pp. 147–50, doi:<a href=\"https://doi.org/10.1051/medsci/2013292011\">10.1051/medsci/2013292011</a>.","short":"J.-L. Maître, H. Berthoumieux, G. Krens, G. Salbreux, F. Julicher, E. Paluch, C.-P.J. Heisenberg, Medecine Sciences 29 (2013) 147–150.","ieee":"J.-L. Maître <i>et al.</i>, “Cell adhesion mechanics of zebrafish gastrulation,” <i>Medecine Sciences</i>, vol. 29, no. 2. Éditions Médicales et Scientifiques, pp. 147–150, 2013.","chicago":"Maître, Jean-Léon, Hélène Berthoumieux, Gabriel Krens, Guillaume Salbreux, Frank Julicher, Ewa Paluch, and Carl-Philipp J Heisenberg. “Cell Adhesion Mechanics of Zebrafish Gastrulation.” <i>Medecine Sciences</i>. Éditions Médicales et Scientifiques, 2013. <a href=\"https://doi.org/10.1051/medsci/2013292011\">https://doi.org/10.1051/medsci/2013292011</a>.","apa":"Maître, J.-L., Berthoumieux, H., Krens, G., Salbreux, G., Julicher, F., Paluch, E., &#38; Heisenberg, C.-P. J. (2013). Cell adhesion mechanics of zebrafish gastrulation. <i>Medecine Sciences</i>. Éditions Médicales et Scientifiques. <a href=\"https://doi.org/10.1051/medsci/2013292011\">https://doi.org/10.1051/medsci/2013292011</a>","ama":"Maître J-L, Berthoumieux H, Krens G, et al. Cell adhesion mechanics of zebrafish gastrulation. <i>Medecine Sciences</i>. 2013;29(2):147-150. doi:<a href=\"https://doi.org/10.1051/medsci/2013292011\">10.1051/medsci/2013292011</a>"},"publisher":"Éditions Médicales et Scientifiques","language":[{"iso":"eng"}],"page":"147 - 150","quality_controlled":"1","title":"Cell adhesion mechanics of zebrafish gastrulation","month":"02","intvolume":"        29","publication_status":"published","oa_version":"None","date_created":"2018-12-11T12:00:08Z","project":[{"_id":"252064B8-B435-11E9-9278-68D0E5697425","grant_number":"HE_3231/6-1","name":"Analysis of the Formation and Function of Different Cell Protusion Types During Cell Migration in Vivo"},{"grant_number":"I 812-B12","name":"Cell Cortex and Germ Layer Formation in Zebrafish Gastrulation","_id":"2527D5CC-B435-11E9-9278-68D0E5697425","call_identifier":"FWF"}],"department":[{"_id":"CaHe"}],"author":[{"id":"48F1E0D8-F248-11E8-B48F-1D18A9856A87","full_name":"Maître, Jean-Léon","orcid":"0000-0002-3688-1474","last_name":"Maître","first_name":"Jean-Léon"},{"first_name":"Hélène","last_name":"Berthoumieux","full_name":"Berthoumieux, Hélène"},{"id":"2B819732-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4761-5996","full_name":"Krens, Gabriel","first_name":"Gabriel","last_name":"Krens"},{"last_name":"Salbreux","first_name":"Guillaume","full_name":"Salbreux, Guillaume"},{"full_name":"Julicher, Frank","last_name":"Julicher","first_name":"Frank"},{"last_name":"Paluch","first_name":"Ewa","full_name":"Paluch, Ewa"},{"last_name":"Heisenberg","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87"}],"issue":"2","_id":"2884","publication":"Medecine Sciences","scopus_import":1},{"oa_version":"None","publication_status":"published","department":[{"_id":"CaHe"}],"date_created":"2018-12-11T12:00:20Z","title":"Anthrax toxin receptor 2a controls mitotic spindle positioning","month":"01","intvolume":"        15","publication":"Nature Cell Biology","_id":"2918","scopus_import":1,"author":[{"full_name":"Castanon, Irinka","first_name":"Irinka","last_name":"Castanon"},{"full_name":"Abrami, Laurence","first_name":"Laurence","last_name":"Abrami"},{"full_name":"Holtzer, Laurent","first_name":"Laurent","last_name":"Holtzer"},{"last_name":"Heisenberg","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Van Der Goot","first_name":"Françoise","full_name":"Van Der Goot, Françoise"},{"first_name":"Marcos","last_name":"González Gaitán","full_name":"González Gaitán, Marcos"}],"issue":"1","publisher":"Nature Publishing Group","page":"28 - 39","quality_controlled":"1","language":[{"iso":"eng"}],"doi":"10.1038/ncb2632","day":"01","abstract":[{"text":"Oriented mitosis is essential during tissue morphogenesis. The Wnt/planar cell polarity (Wnt/PCP) pathway orients mitosis in a number of developmental systems, including dorsal epiblast cell divisions along the animal-vegetal (A-V) axis during zebrafish gastrulation. How Wnt signalling orients the mitotic plane is, however, unknown. Here we show that, in dorsal epiblast cells, anthrax toxin receptor 2a (Antxr2a) accumulates in a polarized cortical cap, which is aligned with the embryonic A-V axis and forecasts the division plane. Filamentous actin (F-actin) also forms an A-V polarized cap, which depends on Wnt/PCP and its effectors RhoA and Rock2. Antxr2a is recruited to the cap by interacting with actin. Antxr2a also interacts with RhoA and together they activate the diaphanous-related formin zDia2. Mechanistically, Antxr2a functions as a Wnt-dependent polarized determinant, which, through the action of RhoA and zDia2, exerts torque on the spindle to align it with the A-V axis.\r\n","lang":"eng"}],"publist_id":"3819","date_updated":"2021-01-12T07:00:41Z","year":"2013","citation":{"ista":"Castanon I, Abrami L, Holtzer L, Heisenberg C-PJ, Van Der Goot F, González Gaitán M. 2013. Anthrax toxin receptor 2a controls mitotic spindle positioning. Nature Cell Biology. 15(1), 28–39.","mla":"Castanon, Irinka, et al. “Anthrax Toxin Receptor 2a Controls Mitotic Spindle Positioning.” <i>Nature Cell Biology</i>, vol. 15, no. 1, Nature Publishing Group, 2013, pp. 28–39, doi:<a href=\"https://doi.org/10.1038/ncb2632\">10.1038/ncb2632</a>.","short":"I. Castanon, L. Abrami, L. Holtzer, C.-P.J. Heisenberg, F. Van Der Goot, M. González Gaitán, Nature Cell Biology 15 (2013) 28–39.","ieee":"I. Castanon, L. Abrami, L. Holtzer, C.-P. J. Heisenberg, F. Van Der Goot, and M. González Gaitán, “Anthrax toxin receptor 2a controls mitotic spindle positioning,” <i>Nature Cell Biology</i>, vol. 15, no. 1. Nature Publishing Group, pp. 28–39, 2013.","chicago":"Castanon, Irinka, Laurence Abrami, Laurent Holtzer, Carl-Philipp J Heisenberg, Françoise Van Der Goot, and Marcos González Gaitán. “Anthrax Toxin Receptor 2a Controls Mitotic Spindle Positioning.” <i>Nature Cell Biology</i>. Nature Publishing Group, 2013. <a href=\"https://doi.org/10.1038/ncb2632\">https://doi.org/10.1038/ncb2632</a>.","apa":"Castanon, I., Abrami, L., Holtzer, L., Heisenberg, C.-P. J., Van Der Goot, F., &#38; González Gaitán, M. (2013). Anthrax toxin receptor 2a controls mitotic spindle positioning. <i>Nature Cell Biology</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/ncb2632\">https://doi.org/10.1038/ncb2632</a>","ama":"Castanon I, Abrami L, Holtzer L, Heisenberg C-PJ, Van Der Goot F, González Gaitán M. Anthrax toxin receptor 2a controls mitotic spindle positioning. <i>Nature Cell Biology</i>. 2013;15(1):28-39. doi:<a href=\"https://doi.org/10.1038/ncb2632\">10.1038/ncb2632</a>"},"date_published":"2013-01-01T00:00:00Z","type":"journal_article","volume":15,"acknowledgement":"This work was supported by the SNSF, the Swiss SystemsX.ch initiative and LipidX-2008/011 (M.G-G. and F.G.v.d.G.), by the Fondation SANTE-Vaduz/Aide au Soutien des Nouvelles Thérapies (F.G.v.d.G.) and by the ERC, the NCCR Frontiers in Genetics and Chemical Biology programmes and the Polish–Swiss research program (M.G-G.).","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","status":"public"},{"publisher":"Wiley-Blackwell","page":"1 - 3","quality_controlled":"1","title":"Neurulation coordinating cell polarisation and lumen formation","intvolume":"        32","publication_status":"published","date_created":"2018-12-11T12:00:20Z","department":[{"_id":"CaHe"}],"author":[{"first_name":"Julien","last_name":"Compagnon","full_name":"Compagnon, Julien","id":"2E3E0988-F248-11E8-B48F-1D18A9856A87"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J","last_name":"Heisenberg","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J"}],"issue":"1","pmid":1,"_id":"2920","scopus_import":1,"volume":32,"abstract":[{"lang":"eng","text":"Cell polarisation in development is a common and fundamental process underlying embryo patterning and morphogenesis, and has been extensively studied over the past years. Our current knowledge of cell polarisation in development is predominantly based on studies that have analysed polarisation of single cells, such as eggs, or cellular aggregates with a stable polarising interface, such as cultured epithelial cells (St Johnston and Ahringer, 2010). However, in embryonic development, particularly of vertebrates, cell polarisation processes often encompass large numbers of cells that are placed within moving and proliferating tissues, and undergo mesenchymal-to-epithelial transitions with a highly complex spatiotemporal choreography. How such intricate cell polarisation processes in embryonic development are achieved has only started to be analysed. By using live imaging of neurulation in the transparent zebrafish embryo, Buckley et al (2012) now describe a novel polarisation strategy by which cells assemble an apical domain in the part of their cell body that intersects with the midline of the forming neural rod. This mechanism, along with the previously described mirror-symmetric divisions (Tawk et al, 2007), is thought to trigger formation of both neural rod midline and lumen."}],"doi":"10.1038/emboj.2012.325","day":"09","external_id":{"pmid":["23211745"]},"date_updated":"2021-01-12T07:00:42Z","year":"2013","citation":{"ama":"Compagnon J, Heisenberg C-PJ. Neurulation coordinating cell polarisation and lumen formation. <i>EMBO Journal</i>. 2013;32(1):1-3. doi:<a href=\"https://doi.org/10.1038/emboj.2012.325\">10.1038/emboj.2012.325</a>","apa":"Compagnon, J., &#38; Heisenberg, C.-P. J. (2013). Neurulation coordinating cell polarisation and lumen formation. <i>EMBO Journal</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1038/emboj.2012.325\">https://doi.org/10.1038/emboj.2012.325</a>","ieee":"J. Compagnon and C.-P. J. Heisenberg, “Neurulation coordinating cell polarisation and lumen formation,” <i>EMBO Journal</i>, vol. 32, no. 1. Wiley-Blackwell, pp. 1–3, 2013.","chicago":"Compagnon, Julien, and Carl-Philipp J Heisenberg. “Neurulation Coordinating Cell Polarisation and Lumen Formation.” <i>EMBO Journal</i>. Wiley-Blackwell, 2013. <a href=\"https://doi.org/10.1038/emboj.2012.325\">https://doi.org/10.1038/emboj.2012.325</a>.","short":"J. Compagnon, C.-P.J. Heisenberg, EMBO Journal 32 (2013) 1–3.","mla":"Compagnon, Julien, and Carl-Philipp J. Heisenberg. “Neurulation Coordinating Cell Polarisation and Lumen Formation.” <i>EMBO Journal</i>, vol. 32, no. 1, Wiley-Blackwell, 2013, pp. 1–3, doi:<a href=\"https://doi.org/10.1038/emboj.2012.325\">10.1038/emboj.2012.325</a>.","ista":"Compagnon J, Heisenberg C-PJ. 2013. Neurulation coordinating cell polarisation and lumen formation. EMBO Journal. 32(1), 1–3."},"language":[{"iso":"eng"}],"month":"01","oa_version":"Submitted Version","publication":"EMBO Journal","status":"public","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","main_file_link":[{"open_access":"1","url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3545307/"}],"publist_id":"3817","oa":1,"date_published":"2013-01-09T00:00:00Z","type":"journal_article"}]