[{"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","doi":"10.1016/j.devcel.2017.09.014","day":"23","publication":"Developmental Cell","ec_funded":1,"language":[{"iso":"eng"}],"publication_identifier":{"issn":["15345807"]},"department":[{"_id":"CaHe"},{"_id":"CaGu"},{"_id":"GaTk"}],"year":"2017","project":[{"call_identifier":"FP7","_id":"25681D80-B435-11E9-9278-68D0E5697425","name":"International IST Postdoc Fellowship Programme","grant_number":"291734"},{"call_identifier":"FWF","grant_number":"I2058","_id":"252DD2A6-B435-11E9-9278-68D0E5697425","name":"Cell segregation in gastrulation: the role of cell fate specification"}],"title":"An effective feedback loop between cell-cell contact duration and morphogen signaling determines cell fate","status":"public","month":"10","isi":1,"publist_id":"6934","oa_version":"None","date_updated":"2024-03-25T23:30:21Z","related_material":{"record":[{"id":"961","relation":"dissertation_contains","status":"public"},{"id":"8350","relation":"dissertation_contains","status":"public"}]},"type":"journal_article","publisher":"Cell Press","citation":{"short":"V. Barone, M. Lang, G. Krens, S. Pradhan, S. Shamipour, K. Sako, M.K. Sikora, C.C. Guet, C.-P.J. Heisenberg, Developmental Cell 43 (2017) 198–211.","mla":"Barone, Vanessa, et al. “An Effective Feedback Loop between Cell-Cell Contact Duration and Morphogen Signaling Determines Cell Fate.” <i>Developmental Cell</i>, vol. 43, no. 2, Cell Press, 2017, pp. 198–211, doi:<a href=\"https://doi.org/10.1016/j.devcel.2017.09.014\">10.1016/j.devcel.2017.09.014</a>.","ama":"Barone V, Lang M, Krens G, et al. An effective feedback loop between cell-cell contact duration and morphogen signaling determines cell fate. <i>Developmental Cell</i>. 2017;43(2):198-211. doi:<a href=\"https://doi.org/10.1016/j.devcel.2017.09.014\">10.1016/j.devcel.2017.09.014</a>","ista":"Barone V, Lang M, Krens G, Pradhan S, Shamipour S, Sako K, Sikora MK, Guet CC, Heisenberg C-PJ. 2017. An effective feedback loop between cell-cell contact duration and morphogen signaling determines cell fate. Developmental Cell. 43(2), 198–211.","apa":"Barone, V., Lang, M., Krens, G., Pradhan, S., Shamipour, S., Sako, K., … Heisenberg, C.-P. J. (2017). An effective feedback loop between cell-cell contact duration and morphogen signaling determines cell fate. <i>Developmental Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.devcel.2017.09.014\">https://doi.org/10.1016/j.devcel.2017.09.014</a>","ieee":"V. Barone <i>et al.</i>, “An effective feedback loop between cell-cell contact duration and morphogen signaling determines cell fate,” <i>Developmental Cell</i>, vol. 43, no. 2. Cell Press, pp. 198–211, 2017.","chicago":"Barone, Vanessa, Moritz Lang, Gabriel Krens, Saurabh Pradhan, Shayan Shamipour, Keisuke Sako, Mateusz K Sikora, Calin C Guet, and Carl-Philipp J Heisenberg. “An Effective Feedback Loop between Cell-Cell Contact Duration and Morphogen Signaling Determines Cell Fate.” <i>Developmental Cell</i>. Cell Press, 2017. <a href=\"https://doi.org/10.1016/j.devcel.2017.09.014\">https://doi.org/10.1016/j.devcel.2017.09.014</a>."},"article_processing_charge":"No","_id":"735","quality_controlled":"1","issue":"2","author":[{"first_name":"Vanessa","full_name":"Barone, Vanessa","id":"419EECCC-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2676-3367","last_name":"Barone"},{"last_name":"Lang","full_name":"Lang, Moritz","first_name":"Moritz","id":"29E0800A-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Krens","id":"2B819732-F248-11E8-B48F-1D18A9856A87","first_name":"Gabriel","full_name":"Krens, Gabriel","orcid":"0000-0003-4761-5996"},{"last_name":"Pradhan","first_name":"Saurabh","full_name":"Pradhan, Saurabh"},{"full_name":"Shamipour, Shayan","first_name":"Shayan","id":"40B34FE2-F248-11E8-B48F-1D18A9856A87","last_name":"Shamipour"},{"last_name":"Sako","first_name":"Keisuke","full_name":"Sako, Keisuke","id":"3BED66BE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6453-8075"},{"last_name":"Sikora","first_name":"Mateusz K","id":"2F74BCDE-F248-11E8-B48F-1D18A9856A87","full_name":"Sikora, Mateusz K"},{"last_name":"Guet","orcid":"0000-0001-6220-2052","first_name":"Calin C","id":"47F8433E-F248-11E8-B48F-1D18A9856A87","full_name":"Guet, Calin C"},{"orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg"}],"page":"198 - 211","volume":43,"date_published":"2017-10-23T00:00:00Z","intvolume":"        43","scopus_import":"1","abstract":[{"lang":"eng","text":"Cell-cell contact formation constitutes an essential step in evolution, leading to the differentiation of specialized cell types. However, remarkably little is known about whether and how the interplay between contact formation and fate specification affects development. Here, we identify a positive feedback loop between cell-cell contact duration, morphogen signaling, and mesendoderm cell-fate specification during zebrafish gastrulation. We show that long-lasting cell-cell contacts enhance the competence of prechordal plate (ppl) progenitor cells to respond to Nodal signaling, required for ppl cell-fate specification. We further show that Nodal signaling promotes ppl cell-cell contact duration, generating a positive feedback loop between ppl cell-cell contact duration and cell-fate specification. Finally, by combining mathematical modeling and experimentation, we show that this feedback determines whether anterior axial mesendoderm cells become ppl or, instead, turn into endoderm. Thus, the interdependent activities of cell-cell signaling and contact formation control fate diversification within the developing embryo."}],"date_created":"2018-12-11T11:48:13Z","external_id":{"isi":["000413443700011"]},"publication_status":"published"},{"tmp":{"image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"file":[{"creator":"dernst","checksum":"64897b0c5373f22273f598e4672c60ff","date_created":"2019-01-18T13:45:40Z","access_level":"open_access","file_id":"5852","relation":"main_file","file_name":"2017_Cell_Samwer.pdf","file_size":17666637,"content_type":"application/pdf","date_updated":"2020-07-14T12:48:08Z"}],"isi":1,"ddc":["570"],"month":"08","status":"public","date_updated":"2023-09-27T10:59:14Z","publist_id":"6848","oa_version":"Published Version","citation":{"ama":"Samwer M, Schneider M, Hoefler R, et al. DNA cross-bridging shapes a single nucleus from a set of mitotic chromosomes. <i>Cell</i>. 2017;170(5):956-972. doi:<a href=\"https://doi.org/10.1016/j.cell.2017.07.038\">10.1016/j.cell.2017.07.038</a>","ista":"Samwer M, Schneider M, Hoefler R, Schmalhorst PS, Jude J, Zuber J, Gerlic D. 2017. DNA cross-bridging shapes a single nucleus from a set of mitotic chromosomes. Cell. 170(5), 956–972.","mla":"Samwer, Matthias, et al. “DNA Cross-Bridging Shapes a Single Nucleus from a Set of Mitotic Chromosomes.” <i>Cell</i>, vol. 170, no. 5, Cell Press, 2017, pp. 956–72, doi:<a href=\"https://doi.org/10.1016/j.cell.2017.07.038\">10.1016/j.cell.2017.07.038</a>.","short":"M. Samwer, M. Schneider, R. Hoefler, P.S. Schmalhorst, J. Jude, J. Zuber, D. Gerlic, Cell 170 (2017) 956–972.","chicago":"Samwer, Matthias, Maximilian Schneider, Rudolf Hoefler, Philipp S Schmalhorst, Julian Jude, Johannes Zuber, and Daniel Gerlic. “DNA Cross-Bridging Shapes a Single Nucleus from a Set of Mitotic Chromosomes.” <i>Cell</i>. Cell Press, 2017. <a href=\"https://doi.org/10.1016/j.cell.2017.07.038\">https://doi.org/10.1016/j.cell.2017.07.038</a>.","ieee":"M. Samwer <i>et al.</i>, “DNA cross-bridging shapes a single nucleus from a set of mitotic chromosomes,” <i>Cell</i>, vol. 170, no. 5. Cell Press, pp. 956–972, 2017.","apa":"Samwer, M., Schneider, M., Hoefler, R., Schmalhorst, P. S., Jude, J., Zuber, J., &#38; Gerlic, D. (2017). DNA cross-bridging shapes a single nucleus from a set of mitotic chromosomes. <i>Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cell.2017.07.038\">https://doi.org/10.1016/j.cell.2017.07.038</a>"},"has_accepted_license":"1","publisher":"Cell Press","article_processing_charge":"No","type":"journal_article","intvolume":"       170","date_published":"2017-08-24T00:00:00Z","page":"956 - 972","volume":170,"author":[{"full_name":"Samwer, Matthias","first_name":"Matthias","last_name":"Samwer"},{"full_name":"Schneider, Maximilian","first_name":"Maximilian","last_name":"Schneider"},{"first_name":"Rudolf","full_name":"Hoefler, Rudolf","last_name":"Hoefler"},{"full_name":"Schmalhorst, Philipp S","id":"309D50DA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-5795-0133","first_name":"Philipp S","last_name":"Schmalhorst"},{"last_name":"Jude","full_name":"Jude, Julian","first_name":"Julian"},{"full_name":"Zuber, Johannes","first_name":"Johannes","last_name":"Zuber"},{"last_name":"Gerlic","first_name":"Daniel","full_name":"Gerlic, Daniel"}],"_id":"803","issue":"5","license":"https://creativecommons.org/licenses/by-nc-nd/4.0/","quality_controlled":"1","file_date_updated":"2020-07-14T12:48:08Z","abstract":[{"text":"Eukaryotic cells store their chromosomes in a single nucleus. This is important to maintain genomic integrity, as chromosomes packaged into separate nuclei (micronuclei) are prone to massive DNA damage. During mitosis, higher eukaryotes disassemble their nucleus and release individualized chromosomes for segregation. How numerous chromosomes subsequently reform a single nucleus has remained unclear. Using image-based screening of human cells, we identified barrier-to-autointegration factor (BAF) as a key factor guiding membranes to form a single nucleus. Unexpectedly, nuclear assembly does not require BAF?s association with inner nuclear membrane proteins but instead relies on BAF?s ability to bridge distant DNA sites. Live-cell imaging and in vitro reconstitution showed that BAF enriches around the mitotic chromosome ensemble to induce a densely cross-bridged chromatin layer that is mechanically stiff and limits membranes to the surface. Our study reveals that BAF-mediated changes in chromosome mechanics underlie nuclear assembly with broad implications for proper genome function.","lang":"eng"}],"date_created":"2018-12-11T11:48:35Z","external_id":{"isi":["000408372400014"]},"publication_status":"published","scopus_import":"1","publication":"Cell","day":"24","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","doi":"10.1016/j.cell.2017.07.038","publication_identifier":{"issn":["00928674"]},"language":[{"iso":"eng"}],"oa":1,"acknowledged_ssus":[{"_id":"Bio"}],"year":"2017","department":[{"_id":"CaHe"}],"title":"DNA cross-bridging shapes a single nucleus from a set of mitotic chromosomes"},{"acknowledgement":"P.S.S. was supported by research fellowship 2811/1-1 from the German Research Foundation (DFG), and M.S. was supported by EMBO Long Term Fellowship ALTF 187-2013 and Grant GC65-32 from the  Interdisciplinary Centre for Mathematical and Computational Modelling (ICM), University of Warsaw, Poland. The authors thank Antje Potthast, Marek Cieplak, Tomasz Włodarski, and Damien Thompson for fruitful discussions and the IST Austria Scientific Computing Facility for support.","department":[{"_id":"CaHe"}],"year":"2017","acknowledged_ssus":[{"_id":"ScienComp"}],"main_file_link":[{"open_access":"1","url":"https://arxiv.org/abs/1704.03773"}],"title":"Overcoming the limitations of the MARTINI force field in simulations of polysaccharides","doi":"10.1021/acs.jctc.7b00374","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","day":"10","publication":"Journal of Chemical Theory and Computation","oa":1,"publication_identifier":{"issn":["15499618"]},"language":[{"iso":"eng"}],"quality_controlled":"1","issue":"10","_id":"804","volume":13,"page":"5039 - 5053","author":[{"last_name":"Schmalhorst","orcid":"0000-0002-5795-0133","id":"309D50DA-F248-11E8-B48F-1D18A9856A87","full_name":"Schmalhorst, Philipp S","first_name":"Philipp S"},{"last_name":"Deluweit","full_name":"Deluweit, Felix","first_name":"Felix"},{"last_name":"Scherrers","full_name":"Scherrers, Roger","first_name":"Roger"},{"last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566"},{"id":"2F74BCDE-F248-11E8-B48F-1D18A9856A87","first_name":"Mateusz K","full_name":"Sikora, Mateusz K","last_name":"Sikora"}],"date_published":"2017-10-10T00:00:00Z","intvolume":"        13","scopus_import":"1","publication_status":"published","external_id":{"isi":["000412965700036"]},"date_created":"2018-12-11T11:48:35Z","abstract":[{"text":"Polysaccharides (carbohydrates) are key regulators of a large number of cell biological processes. However, precise biochemical or genetic manipulation of these often complex structures is laborious and hampers experimental structure–function studies. Molecular Dynamics (MD) simulations provide a valuable alternative tool to generate and test hypotheses on saccharide function. Yet, currently used MD force fields often overestimate the aggregation propensity of polysaccharides, affecting the usability of those simulations. Here we tested MARTINI, a popular coarse-grained (CG) force field for biological macromolecules, for its ability to accurately represent molecular forces between saccharides. To this end, we calculated a thermodynamic solution property, the second virial coefficient of the osmotic pressure (B22). Comparison with light scattering experiments revealed a nonphysical aggregation of a prototypical polysaccharide in MARTINI, pointing at an imbalance of the nonbonded solute–solute, solute–water, and water–water interactions. This finding also applies to smaller oligosaccharides which were all found to aggregate in simulations even at moderate concentrations, well below their solubility limit. Finally, we explored the influence of the Lennard-Jones (LJ) interaction between saccharide molecules and propose a simple scaling of the LJ interaction strength that makes MARTINI more reliable for the simulation of saccharides.","lang":"eng"}],"publist_id":"6847","oa_version":"Submitted Version","date_updated":"2023-09-27T10:58:45Z","status":"public","month":"10","isi":1,"type":"journal_article","article_processing_charge":"No","publisher":"American Chemical Society","citation":{"ama":"Schmalhorst PS, Deluweit F, Scherrers R, Heisenberg C-PJ, Sikora MK. Overcoming the limitations of the MARTINI force field in simulations of polysaccharides. <i>Journal of Chemical Theory and Computation</i>. 2017;13(10):5039-5053. doi:<a href=\"https://doi.org/10.1021/acs.jctc.7b00374\">10.1021/acs.jctc.7b00374</a>","ista":"Schmalhorst PS, Deluweit F, Scherrers R, Heisenberg C-PJ, Sikora MK. 2017. Overcoming the limitations of the MARTINI force field in simulations of polysaccharides. Journal of Chemical Theory and Computation. 13(10), 5039–5053.","short":"P.S. Schmalhorst, F. Deluweit, R. Scherrers, C.-P.J. Heisenberg, M.K. Sikora, Journal of Chemical Theory and Computation 13 (2017) 5039–5053.","mla":"Schmalhorst, Philipp S., et al. “Overcoming the Limitations of the MARTINI Force Field in Simulations of Polysaccharides.” <i>Journal of Chemical Theory and Computation</i>, vol. 13, no. 10, American Chemical Society, 2017, pp. 5039–53, doi:<a href=\"https://doi.org/10.1021/acs.jctc.7b00374\">10.1021/acs.jctc.7b00374</a>.","chicago":"Schmalhorst, Philipp S, Felix Deluweit, Roger Scherrers, Carl-Philipp J Heisenberg, and Mateusz K Sikora. “Overcoming the Limitations of the MARTINI Force Field in Simulations of Polysaccharides.” <i>Journal of Chemical Theory and Computation</i>. American Chemical Society, 2017. <a href=\"https://doi.org/10.1021/acs.jctc.7b00374\">https://doi.org/10.1021/acs.jctc.7b00374</a>.","ieee":"P. S. Schmalhorst, F. Deluweit, R. Scherrers, C.-P. J. Heisenberg, and M. K. Sikora, “Overcoming the limitations of the MARTINI force field in simulations of polysaccharides,” <i>Journal of Chemical Theory and Computation</i>, vol. 13, no. 10. American Chemical Society, pp. 5039–5053, 2017.","apa":"Schmalhorst, P. S., Deluweit, F., Scherrers, R., Heisenberg, C.-P. J., &#38; Sikora, M. K. (2017). Overcoming the limitations of the MARTINI force field in simulations of polysaccharides. <i>Journal of Chemical Theory and Computation</i>. American Chemical Society. <a href=\"https://doi.org/10.1021/acs.jctc.7b00374\">https://doi.org/10.1021/acs.jctc.7b00374</a>"}},{"volume":19,"author":[{"id":"3FE6E4E8-F248-11E8-B48F-1D18A9856A87","full_name":"Smutny, Michael","first_name":"Michael","orcid":"0000-0002-5920-9090","last_name":"Smutny"},{"first_name":"Zsuzsa","full_name":"Ákos, Zsuzsa","last_name":"Ákos"},{"last_name":"Grigolon","full_name":"Grigolon, Silvia","first_name":"Silvia"},{"last_name":"Shamipour","id":"40B34FE2-F248-11E8-B48F-1D18A9856A87","full_name":"Shamipour, Shayan","first_name":"Shayan"},{"full_name":"Ruprecht, Verena","first_name":"Verena","last_name":"Ruprecht"},{"first_name":"Daniel","orcid":"0000-0001-5199-9940","id":"31C42484-F248-11E8-B48F-1D18A9856A87","full_name":"Capek, Daniel","last_name":"Capek"},{"full_name":"Behrndt, Martin","first_name":"Martin","id":"3ECECA3A-F248-11E8-B48F-1D18A9856A87","last_name":"Behrndt"},{"last_name":"Papusheva","id":"41DB591E-F248-11E8-B48F-1D18A9856A87","first_name":"Ekaterina","full_name":"Papusheva, Ekaterina"},{"last_name":"Tada","full_name":"Tada, Masazumi","first_name":"Masazumi"},{"full_name":"Hof, Björn","first_name":"Björn","id":"3A374330-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2057-2754","last_name":"Hof"},{"full_name":"Vicsek, Tamás","first_name":"Tamás","last_name":"Vicsek"},{"first_name":"Guillaume","full_name":"Salbreux, Guillaume","last_name":"Salbreux"},{"last_name":"Heisenberg","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J"}],"page":"306 - 317","intvolume":"        19","date_published":"2017-03-27T00:00:00Z","_id":"661","quality_controlled":"1","abstract":[{"lang":"eng","text":"During embryonic development, mechanical forces are essential for cellular rearrangements driving tissue morphogenesis. Here, we show that in the early zebrafish embryo, friction forces are generated at the interface between anterior axial mesoderm (prechordal plate, ppl) progenitors migrating towards the animal pole and neurectoderm progenitors moving in the opposite direction towards the vegetal pole of the embryo. These friction forces lead to global rearrangement of cells within the neurectoderm and determine the position of the neural anlage. Using a combination of experiments and simulations, we show that this process depends on hydrodynamic coupling between neurectoderm and ppl as a result of E-cadherin-mediated adhesion between those tissues. Our data thus establish the emergence of friction forces at the interface between moving tissues as a critical force-generating process shaping the embryo."}],"publication_status":"published","external_id":{"pmid":["28346437"]},"date_created":"2018-12-11T11:47:46Z","scopus_import":1,"related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"50"},{"status":"public","id":"8350","relation":"dissertation_contains"}]},"status":"public","month":"03","oa_version":"Submitted Version","publist_id":"7074","date_updated":"2024-03-25T23:30:21Z","publisher":"Nature Publishing Group","citation":{"ieee":"M. Smutny <i>et al.</i>, “Friction forces position the neural anlage,” <i>Nature Cell Biology</i>, vol. 19. Nature Publishing Group, pp. 306–317, 2017.","apa":"Smutny, M., Ákos, Z., Grigolon, S., Shamipour, S., Ruprecht, V., Capek, D., … Heisenberg, C.-P. J. (2017). Friction forces position the neural anlage. <i>Nature Cell Biology</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/ncb3492\">https://doi.org/10.1038/ncb3492</a>","chicago":"Smutny, Michael, Zsuzsa Ákos, Silvia Grigolon, Shayan Shamipour, Verena Ruprecht, Daniel Capek, Martin Behrndt, et al. “Friction Forces Position the Neural Anlage.” <i>Nature Cell Biology</i>. Nature Publishing Group, 2017. <a href=\"https://doi.org/10.1038/ncb3492\">https://doi.org/10.1038/ncb3492</a>.","short":"M. Smutny, Z. Ákos, S. Grigolon, S. Shamipour, V. Ruprecht, D. Capek, M. Behrndt, E. Papusheva, M. Tada, B. Hof, T. Vicsek, G. Salbreux, C.-P.J. Heisenberg, Nature Cell Biology 19 (2017) 306–317.","mla":"Smutny, Michael, et al. “Friction Forces Position the Neural Anlage.” <i>Nature Cell Biology</i>, vol. 19, Nature Publishing Group, 2017, pp. 306–17, doi:<a href=\"https://doi.org/10.1038/ncb3492\">10.1038/ncb3492</a>.","ista":"Smutny M, Ákos Z, Grigolon S, Shamipour S, Ruprecht V, Capek D, Behrndt M, Papusheva E, Tada M, Hof B, Vicsek T, Salbreux G, Heisenberg C-PJ. 2017. Friction forces position the neural anlage. Nature Cell Biology. 19, 306–317.","ama":"Smutny M, Ákos Z, Grigolon S, et al. Friction forces position the neural anlage. <i>Nature Cell Biology</i>. 2017;19:306-317. doi:<a href=\"https://doi.org/10.1038/ncb3492\">10.1038/ncb3492</a>"},"type":"journal_article","acknowledged_ssus":[{"_id":"SSU"}],"pmid":1,"department":[{"_id":"CaHe"},{"_id":"BjHo"},{"_id":"Bio"}],"year":"2017","project":[{"call_identifier":"FP7","grant_number":"306589","_id":"25152F3A-B435-11E9-9278-68D0E5697425","name":"Decoding the complexity of turbulence at its origin"},{"call_identifier":"FWF","grant_number":"I 930-B20","_id":"252ABD0A-B435-11E9-9278-68D0E5697425","name":"Control of Epithelial Cell Layer Spreading in Zebrafish"}],"title":"Friction forces position the neural anlage","main_file_link":[{"url":"https://europepmc.org/articles/pmc5635970","open_access":"1"}],"day":"27","publication":"Nature Cell Biology","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","doi":"10.1038/ncb3492","language":[{"iso":"eng"}],"publication_identifier":{"issn":["14657392"]},"oa":1,"ec_funded":1},{"article_type":"original","publication_identifier":{"issn":["09501991"]},"language":[{"iso":"eng"}],"oa":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","doi":"10.1242/dev.144964","day":"15","publication":"Development","title":"Interstitial fluid osmolarity modulates the action of differential tissue surface tension in progenitor cell segregation during gastrulation","department":[{"_id":"Bio"},{"_id":"CaHe"}],"year":"2017","pmid":1,"type":"journal_article","publisher":"Company of Biologists","has_accepted_license":"1","citation":{"chicago":"Krens, Gabriel, Jim Veldhuis, Vanessa Barone, Daniel Capek, Jean-Léon Maître, Wayne Brodland, and Carl-Philipp J Heisenberg. “Interstitial Fluid Osmolarity Modulates the Action of Differential Tissue Surface Tension in Progenitor Cell Segregation during Gastrulation.” <i>Development</i>. Company of Biologists, 2017. <a href=\"https://doi.org/10.1242/dev.144964\">https://doi.org/10.1242/dev.144964</a>.","apa":"Krens, G., Veldhuis, J., Barone, V., Capek, D., Maître, J.-L., Brodland, W., &#38; Heisenberg, C.-P. J. (2017). Interstitial fluid osmolarity modulates the action of differential tissue surface tension in progenitor cell segregation during gastrulation. <i>Development</i>. Company of Biologists. <a href=\"https://doi.org/10.1242/dev.144964\">https://doi.org/10.1242/dev.144964</a>","ieee":"G. Krens <i>et al.</i>, “Interstitial fluid osmolarity modulates the action of differential tissue surface tension in progenitor cell segregation during gastrulation,” <i>Development</i>, vol. 144, no. 10. Company of Biologists, pp. 1798–1806, 2017.","ama":"Krens G, Veldhuis J, Barone V, et al. Interstitial fluid osmolarity modulates the action of differential tissue surface tension in progenitor cell segregation during gastrulation. <i>Development</i>. 2017;144(10):1798-1806. doi:<a href=\"https://doi.org/10.1242/dev.144964\">10.1242/dev.144964</a>","ista":"Krens G, Veldhuis J, Barone V, Capek D, Maître J-L, Brodland W, Heisenberg C-PJ. 2017. Interstitial fluid osmolarity modulates the action of differential tissue surface tension in progenitor cell segregation during gastrulation. Development. 144(10), 1798–1806.","short":"G. Krens, J. Veldhuis, V. Barone, D. Capek, J.-L. Maître, W. Brodland, C.-P.J. Heisenberg, Development 144 (2017) 1798–1806.","mla":"Krens, Gabriel, et al. “Interstitial Fluid Osmolarity Modulates the Action of Differential Tissue Surface Tension in Progenitor Cell Segregation during Gastrulation.” <i>Development</i>, vol. 144, no. 10, Company of Biologists, 2017, pp. 1798–806, doi:<a href=\"https://doi.org/10.1242/dev.144964\">10.1242/dev.144964</a>."},"article_processing_charge":"No","month":"05","status":"public","ddc":["570"],"oa_version":"Published Version","publist_id":"7047","date_updated":"2024-03-25T23:30:13Z","file":[{"creator":"dernst","checksum":"bc25125fb664706cdf180e061429f91d","date_created":"2019-09-24T06:56:22Z","access_level":"open_access","file_id":"6905","relation":"main_file","file_name":"2017_Development_Krens.pdf","file_size":8194516,"content_type":"application/pdf","date_updated":"2020-07-14T12:47:39Z"}],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"related_material":{"record":[{"status":"public","id":"961","relation":"dissertation_contains"},{"relation":"dissertation_contains","id":"50","status":"public"}]},"scopus_import":1,"abstract":[{"lang":"eng","text":"The segregation of different cell types into distinct tissues is a fundamental process in metazoan development. Differences in cell adhesion and cortex tension are commonly thought to drive cell sorting by regulating tissue surface tension (TST). However, the role that differential TST plays in cell segregation within the developing embryo is as yet unclear. Here, we have analyzed the role of differential TST for germ layer progenitor cell segregation during zebrafish gastrulation. Contrary to previous observations that differential TST drives germ layer progenitor cell segregation in vitro, we show that germ layers display indistinguishable TST within the gastrulating embryo, arguing against differential TST driving germ layer progenitor cell segregation in vivo. We further show that the osmolarity of the interstitial fluid (IF) is an important factor that influences germ layer TST in vivo, and that lower osmolarity of the IF compared with standard cell culture medium can explain why germ layers display differential TST in culture but not in vivo. Finally, we show that directed migration of mesendoderm progenitors is required for germ layer progenitor cell segregation and germ layer formation."}],"file_date_updated":"2020-07-14T12:47:39Z","external_id":{"pmid":["28512197"]},"publication_status":"published","date_created":"2018-12-11T11:47:52Z","_id":"676","license":"https://creativecommons.org/licenses/by/4.0/","issue":"10","quality_controlled":"1","author":[{"id":"2B819732-F248-11E8-B48F-1D18A9856A87","first_name":"Gabriel","orcid":"0000-0003-4761-5996","full_name":"Krens, Gabriel","last_name":"Krens"},{"last_name":"Veldhuis","full_name":"Veldhuis, Jim","first_name":"Jim"},{"full_name":"Barone, Vanessa","first_name":"Vanessa","orcid":"0000-0003-2676-3367","id":"419EECCC-F248-11E8-B48F-1D18A9856A87","last_name":"Barone"},{"last_name":"Capek","first_name":"Daniel","id":"31C42484-F248-11E8-B48F-1D18A9856A87","full_name":"Capek, Daniel","orcid":"0000-0001-5199-9940"},{"last_name":"Maître","id":"48F1E0D8-F248-11E8-B48F-1D18A9856A87","first_name":"Jean-Léon","orcid":"0000-0002-3688-1474","full_name":"Maître, Jean-Léon"},{"full_name":"Brodland, Wayne","first_name":"Wayne","last_name":"Brodland"},{"last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J"}],"volume":144,"page":"1798 - 1806","date_published":"2017-05-15T00:00:00Z","intvolume":"       144"},{"date_updated":"2021-01-12T08:08:59Z","doi":"10.1038/ncb3524","publist_id":"7040","oa_version":"None","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","status":"public","month":"05","publication":"Nature Cell Biology","day":"31","type":"journal_article","citation":{"mla":"Petridou, Nicoletta, et al. “Multiscale Force Sensing in Development.” <i>Nature Cell Biology</i>, vol. 19, no. 6, Nature Publishing Group, 2017, pp. 581–88, doi:<a href=\"https://doi.org/10.1038/ncb3524\">10.1038/ncb3524</a>.","short":"N. Petridou, Z.P. Spiro, C.-P.J. Heisenberg, Nature Cell Biology 19 (2017) 581–588.","ama":"Petridou N, Spiro ZP, Heisenberg C-PJ. Multiscale force sensing in development. <i>Nature Cell Biology</i>. 2017;19(6):581-588. doi:<a href=\"https://doi.org/10.1038/ncb3524\">10.1038/ncb3524</a>","ista":"Petridou N, Spiro ZP, Heisenberg C-PJ. 2017. Multiscale force sensing in development. Nature Cell Biology. 19(6), 581–588.","ieee":"N. Petridou, Z. P. Spiro, and C.-P. J. Heisenberg, “Multiscale force sensing in development,” <i>Nature Cell Biology</i>, vol. 19, no. 6. Nature Publishing Group, pp. 581–588, 2017.","apa":"Petridou, N., Spiro, Z. P., &#38; Heisenberg, C.-P. J. (2017). Multiscale force sensing in development. <i>Nature Cell Biology</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/ncb3524\">https://doi.org/10.1038/ncb3524</a>","chicago":"Petridou, Nicoletta, Zoltan P Spiro, and Carl-Philipp J Heisenberg. “Multiscale Force Sensing in Development.” <i>Nature Cell Biology</i>. Nature Publishing Group, 2017. <a href=\"https://doi.org/10.1038/ncb3524\">https://doi.org/10.1038/ncb3524</a>."},"language":[{"iso":"eng"}],"publication_identifier":{"issn":["14657392"]},"publisher":"Nature Publishing Group","project":[{"_id":"25236028-B435-11E9-9278-68D0E5697425","name":"The generation and function of anisotropic tissue tension in zebrafish epiboly (EMBO Fellowship)","grant_number":"ALTF534-2016"}],"quality_controlled":"1","issue":"6","_id":"678","year":"2017","department":[{"_id":"CaHe"}],"intvolume":"        19","date_published":"2017-05-31T00:00:00Z","volume":19,"page":"581 - 588","author":[{"last_name":"Petridou","first_name":"Nicoletta","id":"2A003F6C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8451-1195","full_name":"Petridou, Nicoletta"},{"last_name":"Spiro","full_name":"Spiro, Zoltan P","first_name":"Zoltan P","id":"426AD026-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","last_name":"Heisenberg"}],"scopus_import":1,"date_created":"2018-12-11T11:47:53Z","publication_status":"published","title":"Multiscale force sensing in development","abstract":[{"text":"The seminal observation that mechanical signals can elicit changes in biochemical signalling within cells, a process commonly termed mechanosensation and mechanotransduction, has revolutionized our understanding of the role of cell mechanics in various fundamental biological processes, such as cell motility, adhesion, proliferation and differentiation. In this Review, we will discuss how the interplay and feedback between mechanical and biochemical signals control tissue morphogenesis and cell fate specification in embryonic development.","lang":"eng"}]},{"language":[{"iso":"eng"}],"publication_identifier":{"issn":["09254773"]},"publisher":"Elsevier","citation":{"chicago":"Heisenberg, Carl-Philipp J. “D’Arcy Thompson’s ‘on Growth and Form’: From Soap Bubbles to Tissue Self Organization.” <i>Mechanisms of Development</i>. Elsevier, 2017. <a href=\"https://doi.org/10.1016/j.mod.2017.03.006\">https://doi.org/10.1016/j.mod.2017.03.006</a>.","apa":"Heisenberg, C.-P. J. (2017). D’Arcy Thompson’s ‘on growth and form’: From soap bubbles to tissue self organization. <i>Mechanisms of Development</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.mod.2017.03.006\">https://doi.org/10.1016/j.mod.2017.03.006</a>","ieee":"C.-P. J. Heisenberg, “D’Arcy Thompson’s ‘on growth and form’: From soap bubbles to tissue self organization,” <i>Mechanisms of Development</i>, vol. 145. Elsevier, pp. 32–37, 2017.","ama":"Heisenberg C-PJ. D’Arcy Thompson’s ‘on growth and form’: From soap bubbles to tissue self organization. <i>Mechanisms of Development</i>. 2017;145:32-37. doi:<a href=\"https://doi.org/10.1016/j.mod.2017.03.006\">10.1016/j.mod.2017.03.006</a>","ista":"Heisenberg C-PJ. 2017. D’Arcy Thompson’s ‘on growth and form’: From soap bubbles to tissue self organization. Mechanisms of Development. 145, 32–37.","mla":"Heisenberg, Carl-Philipp J. “D’Arcy Thompson’s ‘on Growth and Form’: From Soap Bubbles to Tissue Self Organization.” <i>Mechanisms of Development</i>, vol. 145, Elsevier, 2017, pp. 32–37, doi:<a href=\"https://doi.org/10.1016/j.mod.2017.03.006\">10.1016/j.mod.2017.03.006</a>.","short":"C.-P.J. Heisenberg, Mechanisms of Development 145 (2017) 32–37."},"type":"journal_article","day":"01","publication":"Mechanisms of Development","publist_id":"7024","oa_version":"None","doi":"10.1016/j.mod.2017.03.006","date_updated":"2021-01-12T08:09:23Z","month":"06","status":"public","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication_status":"published","date_created":"2018-12-11T11:47:55Z","title":"D'Arcy Thompson's ‘on growth and form’: From soap bubbles to tissue self organization","abstract":[{"lang":"eng","text":"Tissues are thought to behave like fluids with a given surface tension. Differences in tissue surface tension (TST) have been proposed to trigger cell sorting and tissue envelopment. D'Arcy Thompson in his seminal book ‘On Growth and Form’ has introduced this concept of differential TST as a key physical mechanism dictating tissue formation and organization within the developing organism. Over the past century, many studies have picked up the concept of differential TST and analyzed the role and cell biological basis of TST in development, underlining the importance and influence of this concept in developmental biology."}],"scopus_import":1,"author":[{"last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566"}],"page":"32 - 37","volume":145,"intvolume":"       145","date_published":"2017-06-01T00:00:00Z","quality_controlled":"1","department":[{"_id":"CaHe"}],"_id":"686","year":"2017"},{"title":"Cell biology: Stretched divisions","year":"2017","department":[{"_id":"CaHe"}],"language":[{"iso":"eng"}],"publication_identifier":{"issn":["00280836"]},"doi":"10.1038/nature21502","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","publication":"Nature","day":"02","scopus_import":"1","publication_status":"published","external_id":{"isi":["000395671500025"]},"date_created":"2018-12-11T11:49:45Z","abstract":[{"text":"Many organ surfaces are covered by a protective epithelial-cell layer. It emerges that such layers are maintained by cell stretching that triggers cell division mediated by the force-sensitive ion-channel protein Piezo1. See Letter p.118","lang":"eng"}],"quality_controlled":"1","issue":"7643","_id":"1025","intvolume":"       543","date_published":"2017-03-02T00:00:00Z","volume":543,"author":[{"last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J"}],"page":"43 - 44","type":"journal_article","article_processing_charge":"No","citation":{"chicago":"Heisenberg, Carl-Philipp J. “Cell Biology: Stretched Divisions.” <i>Nature</i>. Nature Publishing Group, 2017. <a href=\"https://doi.org/10.1038/nature21502\">https://doi.org/10.1038/nature21502</a>.","apa":"Heisenberg, C.-P. J. (2017). Cell biology: Stretched divisions. <i>Nature</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/nature21502\">https://doi.org/10.1038/nature21502</a>","ieee":"C.-P. J. Heisenberg, “Cell biology: Stretched divisions,” <i>Nature</i>, vol. 543, no. 7643. Nature Publishing Group, pp. 43–44, 2017.","ista":"Heisenberg C-PJ. 2017. Cell biology: Stretched divisions. Nature. 543(7643), 43–44.","ama":"Heisenberg C-PJ. Cell biology: Stretched divisions. <i>Nature</i>. 2017;543(7643):43-44. doi:<a href=\"https://doi.org/10.1038/nature21502\">10.1038/nature21502</a>","mla":"Heisenberg, Carl-Philipp J. “Cell Biology: Stretched Divisions.” <i>Nature</i>, vol. 543, no. 7643, Nature Publishing Group, 2017, pp. 43–44, doi:<a href=\"https://doi.org/10.1038/nature21502\">10.1038/nature21502</a>.","short":"C.-P.J. Heisenberg, Nature 543 (2017) 43–44."},"publisher":"Nature Publishing Group","date_updated":"2023-09-22T09:26:59Z","oa_version":"None","publist_id":"6367","isi":1,"status":"public","month":"03"},{"publisher":"Cell Press","has_accepted_license":"1","citation":{"ieee":"H. Morita, S. Grigolon, M. Bock, G. Krens, G. Salbreux, and C.-P. J. Heisenberg, “The physical basis of coordinated tissue spreading in zebrafish gastrulation,” <i>Developmental Cell</i>, vol. 40, no. 4. Cell Press, pp. 354–366, 2017.","apa":"Morita, H., Grigolon, S., Bock, M., Krens, G., Salbreux, G., &#38; Heisenberg, C.-P. J. (2017). The physical basis of coordinated tissue spreading in zebrafish gastrulation. <i>Developmental Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.devcel.2017.01.010\">https://doi.org/10.1016/j.devcel.2017.01.010</a>","chicago":"Morita, Hitoshi, Silvia Grigolon, Martin Bock, Gabriel Krens, Guillaume Salbreux, and Carl-Philipp J Heisenberg. “The Physical Basis of Coordinated Tissue Spreading in Zebrafish Gastrulation.” <i>Developmental Cell</i>. Cell Press, 2017. <a href=\"https://doi.org/10.1016/j.devcel.2017.01.010\">https://doi.org/10.1016/j.devcel.2017.01.010</a>.","mla":"Morita, Hitoshi, et al. “The Physical Basis of Coordinated Tissue Spreading in Zebrafish Gastrulation.” <i>Developmental Cell</i>, vol. 40, no. 4, Cell Press, 2017, pp. 354–66, doi:<a href=\"https://doi.org/10.1016/j.devcel.2017.01.010\">10.1016/j.devcel.2017.01.010</a>.","short":"H. Morita, S. Grigolon, M. Bock, G. Krens, G. Salbreux, C.-P.J. Heisenberg, Developmental Cell 40 (2017) 354–366.","ama":"Morita H, Grigolon S, Bock M, Krens G, Salbreux G, Heisenberg C-PJ. The physical basis of coordinated tissue spreading in zebrafish gastrulation. <i>Developmental Cell</i>. 2017;40(4):354-366. doi:<a href=\"https://doi.org/10.1016/j.devcel.2017.01.010\">10.1016/j.devcel.2017.01.010</a>","ista":"Morita H, Grigolon S, Bock M, Krens G, Salbreux G, Heisenberg C-PJ. 2017. The physical basis of coordinated tissue spreading in zebrafish gastrulation. Developmental Cell. 40(4), 354–366."},"article_processing_charge":"No","type":"journal_article","file":[{"file_size":6866187,"content_type":"application/pdf","date_updated":"2018-12-12T10:10:57Z","file_name":"IST-2017-869-v1+1_1-s2.0-S1534580717300370-main.pdf","relation":"main_file","file_id":"4849","date_created":"2018-12-12T10:10:57Z","access_level":"open_access","creator":"system"}],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"status":"public","month":"02","ddc":["572","597"],"isi":1,"publist_id":"6320","oa_version":"Published Version","date_updated":"2023-09-20T12:06:27Z","abstract":[{"text":"Embryo morphogenesis relies on highly coordinated movements of different tissues. However, remarkably little is known about how tissues coordinate their movements to shape the embryo. In zebrafish embryogenesis, coordinated tissue movements first become apparent during “doming,” when the blastoderm begins to spread over the yolk sac, a process involving coordinated epithelial surface cell layer expansion and mesenchymal deep cell intercalations. Here, we find that active surface cell expansion represents the key process coordinating tissue movements during doming. By using a combination of theory and experiments, we show that epithelial surface cells not only trigger blastoderm expansion by reducing tissue surface tension, but also drive blastoderm thinning by inducing tissue contraction through radial deep cell intercalations. Thus, coordinated tissue expansion and thinning during doming relies on surface cells simultaneously controlling tissue surface tension and radial tissue contraction.","lang":"eng"}],"file_date_updated":"2018-12-12T10:10:57Z","date_created":"2018-12-11T11:49:58Z","external_id":{"isi":["000395368300007"]},"publication_status":"published","scopus_import":"1","page":"354 - 366","author":[{"full_name":"Morita, Hitoshi","first_name":"Hitoshi","id":"4C6E54C6-F248-11E8-B48F-1D18A9856A87","last_name":"Morita"},{"full_name":"Grigolon, Silvia","first_name":"Silvia","last_name":"Grigolon"},{"full_name":"Bock, Martin","first_name":"Martin","last_name":"Bock"},{"last_name":"Krens","id":"2B819732-F248-11E8-B48F-1D18A9856A87","first_name":"Gabriel","full_name":"Krens, Gabriel","orcid":"0000-0003-4761-5996"},{"last_name":"Salbreux","full_name":"Salbreux, Guillaume","first_name":"Guillaume"},{"full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","last_name":"Heisenberg"}],"volume":40,"date_published":"2017-02-27T00:00:00Z","intvolume":"        40","_id":"1067","quality_controlled":"1","issue":"4","language":[{"iso":"eng"}],"publication_identifier":{"issn":["15345807"]},"oa":1,"ec_funded":1,"pubrep_id":"869","day":"27","publication":"Developmental Cell","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","doi":"10.1016/j.devcel.2017.01.010","title":"The physical basis of coordinated tissue spreading in zebrafish gastrulation","acknowledged_ssus":[{"_id":"PreCl"}],"department":[{"_id":"CaHe"}],"year":"2017","project":[{"name":"Developing High-Throughput Bioassays for Human Cancers in Zebrafish","_id":"2524F500-B435-11E9-9278-68D0E5697425","grant_number":"201439","call_identifier":"FP7"}]},{"ec_funded":1,"article_number":"e26792","language":[{"iso":"eng"}],"oa":1,"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","doi":"10.7554/eLife.26792","pubrep_id":"847","day":"19","publication":"eLife","title":"Live tracking of moving samples in confocal microscopy for vertically grown roots","department":[{"_id":"JiFr"},{"_id":"Bio"},{"_id":"CaHe"},{"_id":"EvBe"}],"year":"2017","acknowledgement":"Funding: Marie Curie Actions (FP7/2007-2013 no 291734) to Daniel von Wangenheim; Austrian Science Fund (M 2128-B21) to Matyáš Fendrych; Austrian Science Fund (FWF01_I1774S) to Eva Benková; European Research Council (FP7/2007-2013 no 282300) to Jiří Friml. \r\nThe authors are grateful to the Miba Machine Shop at IST Austria for their contribution to the microscope setup and to Yvonne Kemper for reading, understanding and correcting the manuscript.\r\n#BioimagingFacility","project":[{"grant_number":"291734","_id":"25681D80-B435-11E9-9278-68D0E5697425","name":"International IST Postdoc Fellowship Programme","call_identifier":"FP7"},{"_id":"2572ED28-B435-11E9-9278-68D0E5697425","name":"Molecular basis of root growth inhibition by auxin","grant_number":"M02128","call_identifier":"FWF"},{"_id":"2542D156-B435-11E9-9278-68D0E5697425","name":"Hormone cross-talk drives nutrient dependent plant development","grant_number":"I 1774-B16","call_identifier":"FWF"},{"grant_number":"282300","name":"Polarity and subcellular dynamics in plants","_id":"25716A02-B435-11E9-9278-68D0E5697425","call_identifier":"FP7"}],"acknowledged_ssus":[{"_id":"M-Shop"},{"_id":"Bio"}],"type":"journal_article","has_accepted_license":"1","publisher":"eLife Sciences Publications","citation":{"chicago":"Wangenheim, Daniel von, Robert Hauschild, Matyas Fendrych, Vanessa Barone, Eva Benková, and Jiří Friml. “Live Tracking of Moving Samples in Confocal Microscopy for Vertically Grown Roots.” <i>ELife</i>. eLife Sciences Publications, 2017. <a href=\"https://doi.org/10.7554/eLife.26792\">https://doi.org/10.7554/eLife.26792</a>.","ieee":"D. von Wangenheim, R. Hauschild, M. Fendrych, V. Barone, E. Benková, and J. Friml, “Live tracking of moving samples in confocal microscopy for vertically grown roots,” <i>eLife</i>, vol. 6. eLife Sciences Publications, 2017.","apa":"von Wangenheim, D., Hauschild, R., Fendrych, M., Barone, V., Benková, E., &#38; Friml, J. (2017). Live tracking of moving samples in confocal microscopy for vertically grown roots. <i>ELife</i>. eLife Sciences Publications. <a href=\"https://doi.org/10.7554/eLife.26792\">https://doi.org/10.7554/eLife.26792</a>","ista":"von Wangenheim D, Hauschild R, Fendrych M, Barone V, Benková E, Friml J. 2017. Live tracking of moving samples in confocal microscopy for vertically grown roots. eLife. 6, e26792.","ama":"von Wangenheim D, Hauschild R, Fendrych M, Barone V, Benková E, Friml J. Live tracking of moving samples in confocal microscopy for vertically grown roots. <i>eLife</i>. 2017;6. doi:<a href=\"https://doi.org/10.7554/eLife.26792\">10.7554/eLife.26792</a>","mla":"von Wangenheim, Daniel, et al. “Live Tracking of Moving Samples in Confocal Microscopy for Vertically Grown Roots.” <i>ELife</i>, vol. 6, e26792, eLife Sciences Publications, 2017, doi:<a href=\"https://doi.org/10.7554/eLife.26792\">10.7554/eLife.26792</a>.","short":"D. von Wangenheim, R. Hauschild, M. Fendrych, V. Barone, E. Benková, J. Friml, ELife 6 (2017)."},"article_processing_charge":"Yes","month":"06","status":"public","isi":1,"ddc":["570"],"publist_id":"6471","oa_version":"Published Version","date_updated":"2025-05-07T11:12:33Z","file":[{"file_id":"5315","date_created":"2018-12-12T10:17:57Z","access_level":"open_access","checksum":"9af3398cb0d81f99d79016a616df22e9","creator":"system","file_size":19581847,"content_type":"application/pdf","date_updated":"2020-07-14T12:48:15Z","file_name":"IST-2017-847-v1+1_elife-26792-v2.pdf","relation":"main_file"}],"related_material":{"record":[{"status":"public","id":"5566","relation":"popular_science"}]},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"scopus_import":"1","file_date_updated":"2020-07-14T12:48:15Z","abstract":[{"text":"Roots navigate through soil integrating environmental signals to orient their growth. The Arabidopsis root is a widely used model for developmental, physiological and cell biological studies. Live imaging greatly aids these efforts, but the horizontal sample position and continuous root tip displacement present significant difficulties. Here, we develop a confocal microscope setup for vertical sample mounting and integrated directional illumination. We present TipTracker – a custom software for automatic tracking of diverse moving objects usable on various microscope setups. Combined, this enables observation of root tips growing along the natural gravity vector over prolonged periods of time, as well as the ability to induce rapid gravity or light stimulation. We also track migrating cells in the developing zebrafish embryo, demonstrating the utility of this system in the acquisition of high-resolution data sets of dynamic samples. We provide detailed descriptions of the tools enabling the easy implementation on other microscopes.","lang":"eng"}],"publication_status":"published","external_id":{"isi":["000404728300001"]},"date_created":"2018-12-11T11:49:21Z","_id":"946","quality_controlled":"1","author":[{"orcid":"0000-0002-6862-1247","first_name":"Daniel","id":"49E91952-F248-11E8-B48F-1D18A9856A87","full_name":"Von Wangenheim, Daniel","last_name":"Von Wangenheim"},{"last_name":"Hauschild","first_name":"Robert","full_name":"Hauschild, Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9843-3522"},{"last_name":"Fendrych","first_name":"Matyas","id":"43905548-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-9767-8699","full_name":"Fendrych, Matyas"},{"full_name":"Barone, Vanessa","orcid":"0000-0003-2676-3367","id":"419EECCC-F248-11E8-B48F-1D18A9856A87","first_name":"Vanessa","last_name":"Barone"},{"orcid":"0000-0002-8510-9739","full_name":"Benková, Eva","first_name":"Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","last_name":"Benková"},{"full_name":"Friml, Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jirí","orcid":"0000-0002-8302-7596","last_name":"Friml"}],"volume":6,"intvolume":"         6","date_published":"2017-06-19T00:00:00Z"},{"acknowledgement":"Many people accompanied me during this trip: I would not have reached my destination nor \r\nenjoyed the travelling without them. First of all, thanks to CP. Thanks for making me part of \r\nyour team, always full of diverse, interesting and incredibly competent people and thanks for \r\nall  the  good  science  I  witnessed  and  participated  in.  It  has  been  a \r\nblast,  an  incredibly \r\nexciting  one!  Thanks  to  JLo,  for  teaching  me  how  to  master  my  pipettes  and  showing  me \r\nthat science is a lot of fun. Many, many thanks to Gabby for teaching me basically everything \r\nabout  zebrafish  and  being  always  there  to  advice,  sugge\r\nst,  support...and  play  fussball! \r\nThank you to Julien, for the critical eye on things, Pedro, for all the invaluable feedback and \r\nthe amazing kicker matches, and Keisuke, for showing me the light, and to the three of them \r\ntogether  for  all  the  good  laughs  we\r\nhad.  My  start  in  Vienna  would  have  been  a  lot  more \r\ndifficult  without  you  guys.  Also  it  would  not  have  been  possible  without  Elena  and  Inês: \r\nthanks  for  helping  setting  up  this  lab  and  for  the  dinners  in  Gugging.  Thanks  to  Martin,  for \r\nhelping  me  understand \r\nthe  physics  behind  biology.  Thanks  to  Philipp,  for  the  interest  and \r\nadvice, and to Michael, for the Viennise take on things. Thanks to Julia, for putting up with \r\nbeing our technician and becoming a friend in the process. And now to the newest members \r\nof th\r\ne lab. Thanks to Daniel for the enthusiasm and the neverending energy and for all your \r\nhelp over the years: thank you! To Jana, for showing me that one doesn’t give up, no matter \r\nwhat.  To  Shayan,  for  being  such  a  motivated  student.  To  Matt,  for  helping  out\r\nwith  coding \r\nand for finding punk solutions to data analysis problems. Thanks to all the members of the \r\nlab, Verena, Hitoshi, Silvia, Conny, Karla, Nicoletta, Zoltan, Peng, Benoit, Roland, Yuuta and \r\nFeyza,  for  the  wonderful  atmosphere  in  the  lab.  Many  than\r\nks  to  Koni  and  Deborah:  doing \r\nexperiments would have been much more difficult without your help. Special thanks to Katjia \r\nfor  setting  up  an  amazing  imaging  facility  and  for  building  the  best  team,  Robert,  Nasser, \r\nAnna and Doreen: thank you for putting up w\r\nith all the late sortings and for helping with all \r\nthe technical problems. Thanks to Eva, Verena and Matthias for keeping the fish happy. Big \r\nthanks to Harald Janovjak for being a present and helpful committee member over the years \r\nand  to  Patrick  Lemaire  f\r\nor  the  helpful  insight  and  extremely  interesting  discussion  we  had \r\nabout  the  project.  Also,  this  journey  would  not  have  been  the  same  without  all  the  friends \r\nthat I met in Dresden and then in Vienna: Daniele, Claire, Kuba, Steffi, Harold, Dejan, Irene, \r\nFab\r\nienne, Hande, Tiago, Marianne, Jon, Srdjan, Branca, Uli, Murat, Alex, Conny, Christoph, \r\nCaro, Simone, Barbara, Felipe, Dama, Jose, Hubert and many others that filled my days with \r\nfun and support. A special thank to my family, always close even if they are \r\nkilometers away. \r\nGrazie  ai  miei  fratelli,  Nunzio  e  William,  e  alla  mia  mamma,  per  essermi  sempre  vicini  pur \r\nvivendo a chilometri di distanza. And, last but not least, thanks to Moritz, for putting up with \r\nthe crazy life of a scientist, the living apart for\r\nso long, never knowing when things are going \r\nto happen. Thanks for being a great partner and my number one fan!","department":[{"_id":"CaHe"}],"year":"2017","alternative_title":["ISTA Thesis"],"title":"Cell adhesion and cell fate: An effective feedback loop during zebrafish gastrulation","day":"01","supervisor":[{"full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg"}],"pubrep_id":"825","doi":"10.15479/AT:ISTA:th_825","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","oa":1,"language":[{"iso":"eng"}],"publication_identifier":{"issn":["2663-337X"]},"degree_awarded":"PhD","author":[{"last_name":"Barone","id":"419EECCC-F248-11E8-B48F-1D18A9856A87","first_name":"Vanessa","full_name":"Barone, Vanessa","orcid":"0000-0003-2676-3367"}],"page":"109","date_published":"2017-03-01T00:00:00Z","_id":"961","date_created":"2018-12-11T11:49:25Z","publication_status":"published","file_date_updated":"2020-07-14T12:48:16Z","abstract":[{"text":"Cell-cell  contact  formation  constitutes  the  first  step  in  the  emergence  of  multicellularity  in evolution, thereby  allowing  the  differentiation  of  specialized  cell  types.  In  metazoan development, cell-cell contact formation is thought to influence cell fate specification, and cell   fate   specification   has   been   implicated   in   cell-cell  contact formation.   However, remarkably little is yet known about whether and how the interaction and feedback between cell-cell contact formation and cell fate specification affect development. Here we identify a positive  feedback  loop  between  cell-cell  contact  duration,  morphogen  signaling  and mesendoderm  cell  fate  specification  during  zebrafish  gastrulation.  We  show  that  long lasting cell-cell contacts enhance the competence of prechordal plate (ppl) progenitor cells to  respond  to  Nodal  signaling,  required  for  proper  ppl  cell  fate  specification.  We  further show  that  Nodal  signalling  romotes  ppl  cell-cell  contact  duration,  thereby  generating  an effective  positive  feedback  loop  between  ppl  cell-cell  contact  duration  and  cell  fate specification. Finally, by using a combination of theoretical modeling and experimentation, we  show  that  this  feedback  loop  determines  whether  anterior  axial  mesendoderm  cells become  ppl  progenitors  or,  instead,  turn  into  endoderm  progenitors.  Our  findings  reveal that  the  gene  regulatory  networks  leading  to  cell  fate  diversification  within  the  developing embryo  are  controlled  by  the  interdependent  activities  of  cell-cell  signaling  and  contact formation.","lang":"eng"}],"file":[{"file_name":"2017_Barone_thesis_final.docx","file_size":14497822,"content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","date_updated":"2020-07-14T12:48:16Z","relation":"source_file","file_id":"6205","creator":"dernst","checksum":"242f88c87f2cf267bf05049fa26a687b","date_created":"2019-04-05T08:36:52Z","access_level":"closed"},{"relation":"main_file","file_size":14995941,"date_updated":"2020-07-14T12:48:16Z","content_type":"application/pdf","file_name":"2017_Barone_thesis_.pdf","date_created":"2019-04-05T08:36:52Z","access_level":"open_access","creator":"dernst","checksum":"ba5b0613ed8bade73a409acdd880fb8a","file_id":"6206"}],"related_material":{"record":[{"relation":"part_of_dissertation","id":"1100","status":"public"},{"relation":"part_of_dissertation","id":"1537","status":"public"},{"id":"1912","relation":"part_of_dissertation","status":"public"},{"status":"public","id":"2926","relation":"part_of_dissertation"},{"status":"public","id":"3246","relation":"part_of_dissertation"},{"status":"public","id":"676","relation":"part_of_dissertation"},{"relation":"part_of_dissertation","id":"735","status":"public"}]},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"publist_id":"6444","oa_version":"Published Version","date_updated":"2023-09-27T14:16:45Z","month":"03","status":"public","ddc":["570","590"],"article_processing_charge":"No","has_accepted_license":"1","publisher":"Institute of Science and Technology Austria","citation":{"ama":"Barone V. Cell adhesion and cell fate: An effective feedback loop during zebrafish gastrulation. 2017. doi:<a href=\"https://doi.org/10.15479/AT:ISTA:th_825\">10.15479/AT:ISTA:th_825</a>","ista":"Barone V. 2017. Cell adhesion and cell fate: An effective feedback loop during zebrafish gastrulation. Institute of Science and Technology Austria.","mla":"Barone, Vanessa. <i>Cell Adhesion and Cell Fate: An Effective Feedback Loop during Zebrafish Gastrulation</i>. Institute of Science and Technology Austria, 2017, doi:<a href=\"https://doi.org/10.15479/AT:ISTA:th_825\">10.15479/AT:ISTA:th_825</a>.","short":"V. Barone, Cell Adhesion and Cell Fate: An Effective Feedback Loop during Zebrafish Gastrulation, Institute of Science and Technology Austria, 2017.","chicago":"Barone, Vanessa. “Cell Adhesion and Cell Fate: An Effective Feedback Loop during Zebrafish Gastrulation.” Institute of Science and Technology Austria, 2017. <a href=\"https://doi.org/10.15479/AT:ISTA:th_825\">https://doi.org/10.15479/AT:ISTA:th_825</a>.","ieee":"V. Barone, “Cell adhesion and cell fate: An effective feedback loop during zebrafish gastrulation,” Institute of Science and Technology Austria, 2017.","apa":"Barone, V. (2017). <i>Cell adhesion and cell fate: An effective feedback loop during zebrafish gastrulation</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT:ISTA:th_825\">https://doi.org/10.15479/AT:ISTA:th_825</a>"},"type":"dissertation"},{"oa_version":"None","publist_id":"6095","doi":"10.1103/PhysRevLett.116.028102","date_updated":"2021-01-12T06:49:19Z","month":"01","status":"public","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","day":"15","publication":"Physical Review Letters","article_number":"028102","type":"journal_article","language":[{"iso":"eng"}],"publisher":"American Physical Society","citation":{"ista":"Callan Jones A, Ruprecht V, Wieser S, Heisenberg C-PJ, Voituriez R. 2016. Cortical flow-driven shapes of nonadherent cells. Physical Review Letters. 116(2), 028102.","ama":"Callan Jones A, Ruprecht V, Wieser S, Heisenberg C-PJ, Voituriez R. Cortical flow-driven shapes of nonadherent cells. <i>Physical Review Letters</i>. 2016;116(2). doi:<a href=\"https://doi.org/10.1103/PhysRevLett.116.028102\">10.1103/PhysRevLett.116.028102</a>","short":"A. Callan Jones, V. Ruprecht, S. Wieser, C.-P.J. Heisenberg, R. Voituriez, Physical Review Letters 116 (2016).","mla":"Callan Jones, Andrew, et al. “Cortical Flow-Driven Shapes of Nonadherent Cells.” <i>Physical Review Letters</i>, vol. 116, no. 2, 028102, American Physical Society, 2016, doi:<a href=\"https://doi.org/10.1103/PhysRevLett.116.028102\">10.1103/PhysRevLett.116.028102</a>.","chicago":"Callan Jones, Andrew, Verena Ruprecht, Stefan Wieser, Carl-Philipp J Heisenberg, and Raphaël Voituriez. “Cortical Flow-Driven Shapes of Nonadherent Cells.” <i>Physical Review Letters</i>. American Physical Society, 2016. <a href=\"https://doi.org/10.1103/PhysRevLett.116.028102\">https://doi.org/10.1103/PhysRevLett.116.028102</a>.","ieee":"A. Callan Jones, V. Ruprecht, S. Wieser, C.-P. J. Heisenberg, and R. Voituriez, “Cortical flow-driven shapes of nonadherent cells,” <i>Physical Review Letters</i>, vol. 116, no. 2. American Physical Society, 2016.","apa":"Callan Jones, A., Ruprecht, V., Wieser, S., Heisenberg, C.-P. J., &#38; Voituriez, R. (2016). Cortical flow-driven shapes of nonadherent cells. <i>Physical Review Letters</i>. American Physical Society. <a href=\"https://doi.org/10.1103/PhysRevLett.116.028102\">https://doi.org/10.1103/PhysRevLett.116.028102</a>"},"acknowledgement":"V. R. acknowledges support by the Austrian Science Fund (FWF): (Grant No. T560-B17).","quality_controlled":"1","issue":"2","project":[{"call_identifier":"FWF","_id":"2529486C-B435-11E9-9278-68D0E5697425","name":"Cell- and Tissue Mechanics in Zebrafish Germ Layer Formation","grant_number":"T 560-B17"}],"department":[{"_id":"CaHe"}],"year":"2016","_id":"1239","volume":116,"author":[{"full_name":"Callan Jones, Andrew","first_name":"Andrew","last_name":"Callan Jones"},{"orcid":"0000-0003-4088-8633","id":"4D71A03A-F248-11E8-B48F-1D18A9856A87","full_name":"Ruprecht, Verena","first_name":"Verena","last_name":"Ruprecht"},{"first_name":"Stefan","full_name":"Wieser, Stefan","id":"355AA5A0-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-2670-2217","last_name":"Wieser"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","last_name":"Heisenberg"},{"full_name":"Voituriez, Raphaël","first_name":"Raphaël","last_name":"Voituriez"}],"intvolume":"       116","date_published":"2016-01-15T00:00:00Z","scopus_import":1,"date_created":"2018-12-11T11:50:53Z","publication_status":"published","title":"Cortical flow-driven shapes of nonadherent cells","abstract":[{"lang":"eng","text":"Nonadherent polarized cells have been observed to have a pearlike, elongated shape. Using a minimal model that describes the cell cortex as a thin layer of contractile active gel, we show that the anisotropy of active stresses, controlled by cortical viscosity and filament ordering, can account for this morphology. The predicted shapes can be determined from the flow pattern only; they prove to be independent of the mechanism at the origin of the cortical flow, and are only weakly sensitive to the cytoplasmic rheology. In the case of actin flows resulting from a contractile instability, we propose a phase diagram of three-dimensional cell shapes that encompasses nonpolarized spherical, elongated, as well as oblate shapes, all of which have been observed in experiment."}]},{"oa":1,"language":[{"iso":"eng"}],"doi":"10.1016/j.bpj.2016.02.013","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","publication":"Biophysical Journal","day":"29","pubrep_id":"706","title":"Determining physical properties of the cell cortex","project":[{"call_identifier":"FWF","grant_number":"I 930-B20","name":"Control of Epithelial Cell Layer Spreading in Zebrafish","_id":"252ABD0A-B435-11E9-9278-68D0E5697425"}],"acknowledgement":"S.W.G. acknowledges support by grant no. 281903 from the European Research Council and by grant No. GR-7271/2-1 from the Deutsche Forschungsgemeinschaft. S.W.G. and C.-P.H. acknowledge support through a grant from the Fonds zur Förderung der Wissenschaftlichen Forschung and the Deutsche Forschungsgemeinschaft (No. I930-B20). We are grateful to Daniel Dickinson for providing the LP133 C. elegans strain. We thank G. Salbreux, V. K. Krishnamurthy, and J. S. Bois for fruitful discussions.","year":"2016","department":[{"_id":"CaHe"}],"type":"journal_article","citation":{"chicago":"Saha, Arnab, Masatoshi Nishikawa, Martin Behrndt, Carl-Philipp J Heisenberg, Frank Julicher, and Stephan Grill. “Determining Physical Properties of the Cell Cortex.” <i>Biophysical Journal</i>. Biophysical Society, 2016. <a href=\"https://doi.org/10.1016/j.bpj.2016.02.013\">https://doi.org/10.1016/j.bpj.2016.02.013</a>.","ieee":"A. Saha, M. Nishikawa, M. Behrndt, C.-P. J. Heisenberg, F. Julicher, and S. Grill, “Determining physical properties of the cell cortex,” <i>Biophysical Journal</i>, vol. 110, no. 6. Biophysical Society, pp. 1421–1429, 2016.","apa":"Saha, A., Nishikawa, M., Behrndt, M., Heisenberg, C.-P. J., Julicher, F., &#38; Grill, S. (2016). Determining physical properties of the cell cortex. <i>Biophysical Journal</i>. Biophysical Society. <a href=\"https://doi.org/10.1016/j.bpj.2016.02.013\">https://doi.org/10.1016/j.bpj.2016.02.013</a>","ama":"Saha A, Nishikawa M, Behrndt M, Heisenberg C-PJ, Julicher F, Grill S. Determining physical properties of the cell cortex. <i>Biophysical Journal</i>. 2016;110(6):1421-1429. doi:<a href=\"https://doi.org/10.1016/j.bpj.2016.02.013\">10.1016/j.bpj.2016.02.013</a>","ista":"Saha A, Nishikawa M, Behrndt M, Heisenberg C-PJ, Julicher F, Grill S. 2016. Determining physical properties of the cell cortex. Biophysical Journal. 110(6), 1421–1429.","mla":"Saha, Arnab, et al. “Determining Physical Properties of the Cell Cortex.” <i>Biophysical Journal</i>, vol. 110, no. 6, Biophysical Society, 2016, pp. 1421–29, doi:<a href=\"https://doi.org/10.1016/j.bpj.2016.02.013\">10.1016/j.bpj.2016.02.013</a>.","short":"A. Saha, M. Nishikawa, M. Behrndt, C.-P.J. Heisenberg, F. Julicher, S. Grill, Biophysical Journal 110 (2016) 1421–1429."},"publisher":"Biophysical Society","has_accepted_license":"1","date_updated":"2021-01-12T06:49:23Z","publist_id":"6079","oa_version":"Published Version","ddc":["572","576"],"month":"03","status":"public","tmp":{"image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"file":[{"relation":"main_file","date_updated":"2020-07-14T12:44:41Z","content_type":"application/pdf","file_size":1965645,"file_name":"IST-2016-706-v1+1_1-s2.0-S0006349516001582-main.pdf","access_level":"open_access","date_created":"2018-12-12T10:10:54Z","checksum":"c408cf2e25a25c8d711cffea524bda55","creator":"system","file_id":"4845"}],"scopus_import":1,"date_created":"2018-12-11T11:50:56Z","publication_status":"published","abstract":[{"text":"Actin and myosin assemble into a thin layer of a highly dynamic network underneath the membrane of eukaryotic cells. This network generates the forces that drive cell- and tissue-scale morphogenetic processes. The effective material properties of this active network determine large-scale deformations and other morphogenetic events. For example, the characteristic time of stress relaxation (the Maxwell time τM) in the actomyosin sets the timescale of large-scale deformation of the cortex. Similarly, the characteristic length of stress propagation (the hydrodynamic length λ) sets the length scale of slow deformations, and a large hydrodynamic length is a prerequisite for long-ranged cortical flows. Here we introduce a method to determine physical parameters of the actomyosin cortical layer in vivo directly from laser ablation experiments. For this we investigate the cortical response to laser ablation in the one-cell-stage Caenorhabditis elegans embryo and in the gastrulating zebrafish embryo. These responses can be interpreted using a coarse-grained physical description of the cortex in terms of a two-dimensional thin film of an active viscoelastic gel. To determine the Maxwell time τM, the hydrodynamic length λ, the ratio of active stress ζΔμ, and per-area friction γ, we evaluated the response to laser ablation in two different ways: by quantifying flow and density fields as a function of space and time, and by determining the time evolution of the shape of the ablated region. Importantly, both methods provide best-fit physical parameters that are in close agreement with each other and that are similar to previous estimates in the two systems. Our method provides an accurate and robust means for measuring physical parameters of the actomyosin cortical layer. It can be useful for investigations of actomyosin mechanics at the cellular-scale, but also for providing insights into the active mechanics processes that govern tissue-scale morphogenesis.","lang":"eng"}],"file_date_updated":"2020-07-14T12:44:41Z","issue":"6","quality_controlled":"1","_id":"1249","intvolume":"       110","date_published":"2016-03-29T00:00:00Z","page":"1421 - 1429","author":[{"first_name":"Arnab","full_name":"Saha, Arnab","last_name":"Saha"},{"last_name":"Nishikawa","first_name":"Masatoshi","full_name":"Nishikawa, Masatoshi"},{"last_name":"Behrndt","first_name":"Martin","id":"3ECECA3A-F248-11E8-B48F-1D18A9856A87","full_name":"Behrndt, Martin"},{"first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg"},{"first_name":"Frank","full_name":"Julicher, Frank","last_name":"Julicher"},{"full_name":"Grill, Stephan","first_name":"Stephan","last_name":"Grill"}],"volume":110},{"citation":{"chicago":"Diz Muñoz, Alba, Pawel Romanczuk, Weimiao Yu, Martin Bergert, Kenzo Ivanovitch, Guillame Salbreux, Carl-Philipp J Heisenberg, and Ewa Paluch. “Steering Cell Migration by Alternating Blebs and Actin-Rich Protrusions.” <i>BMC Biology</i>. BioMed Central, 2016. <a href=\"https://doi.org/10.1186/s12915-016-0294-x\">https://doi.org/10.1186/s12915-016-0294-x</a>.","ieee":"A. Diz Muñoz <i>et al.</i>, “Steering cell migration by alternating blebs and actin-rich protrusions,” <i>BMC Biology</i>, vol. 14, no. 1. BioMed Central, 2016.","apa":"Diz Muñoz, A., Romanczuk, P., Yu, W., Bergert, M., Ivanovitch, K., Salbreux, G., … Paluch, E. (2016). Steering cell migration by alternating blebs and actin-rich protrusions. <i>BMC Biology</i>. BioMed Central. <a href=\"https://doi.org/10.1186/s12915-016-0294-x\">https://doi.org/10.1186/s12915-016-0294-x</a>","ista":"Diz Muñoz A, Romanczuk P, Yu W, Bergert M, Ivanovitch K, Salbreux G, Heisenberg C-PJ, Paluch E. 2016. Steering cell migration by alternating blebs and actin-rich protrusions. BMC Biology. 14(1), 74.","ama":"Diz Muñoz A, Romanczuk P, Yu W, et al. Steering cell migration by alternating blebs and actin-rich protrusions. <i>BMC Biology</i>. 2016;14(1). doi:<a href=\"https://doi.org/10.1186/s12915-016-0294-x\">10.1186/s12915-016-0294-x</a>","mla":"Diz Muñoz, Alba, et al. “Steering Cell Migration by Alternating Blebs and Actin-Rich Protrusions.” <i>BMC Biology</i>, vol. 14, no. 1, 74, BioMed Central, 2016, doi:<a href=\"https://doi.org/10.1186/s12915-016-0294-x\">10.1186/s12915-016-0294-x</a>.","short":"A. Diz Muñoz, P. Romanczuk, W. Yu, M. Bergert, K. Ivanovitch, G. Salbreux, C.-P.J. Heisenberg, E. Paluch, BMC Biology 14 (2016)."},"has_accepted_license":"1","publisher":"BioMed Central","type":"journal_article","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"file":[{"date_created":"2018-12-12T10:13:20Z","access_level":"open_access","checksum":"0bfa484ac69a0a560fb9a4589aeda7f6","creator":"system","file_id":"5002","relation":"main_file","file_size":1875695,"date_updated":"2020-07-14T12:44:42Z","content_type":"application/pdf","file_name":"IST-2016-695-v1+1_s12915-016-0294-x.pdf"}],"ddc":["572","576"],"status":"public","month":"09","date_updated":"2021-01-12T06:49:32Z","publist_id":"6049","oa_version":"Published Version","abstract":[{"lang":"eng","text":"Background: High directional persistence is often assumed to enhance the efficiency of chemotactic migration. Yet, cells in vivo usually display meandering trajectories with relatively low directional persistence, and the control and function of directional persistence during cell migration in three-dimensional environments are poorly understood. Results: Here, we use mesendoderm progenitors migrating during zebrafish gastrulation as a model system to investigate the control of directional persistence during migration in vivo. We show that progenitor cells alternate persistent run phases with tumble phases that result in cell reorientation. Runs are characterized by the formation of directed actin-rich protrusions and tumbles by enhanced blebbing. Increasing the proportion of actin-rich protrusions or blebs leads to longer or shorter run phases, respectively. Importantly, both reducing and increasing run phases result in larger spatial dispersion of the cells, indicative of reduced migration precision. A physical model quantitatively recapitulating the migratory behavior of mesendoderm progenitors indicates that the ratio of tumbling to run times, and thus the specific degree of directional persistence of migration, are critical for optimizing migration precision. Conclusions: Together, our experiments and model provide mechanistic insight into the control of migration directionality for cells moving in three-dimensional environments that combine different protrusion types, whereby the proportion of blebs to actin-rich protrusions determines the directional persistence and precision of movement by regulating the ratio of tumbling to run times."}],"file_date_updated":"2020-07-14T12:44:42Z","date_created":"2018-12-11T11:51:04Z","publication_status":"published","scopus_import":1,"date_published":"2016-09-02T00:00:00Z","intvolume":"        14","volume":14,"author":[{"last_name":"Diz Muñoz","full_name":"Diz Muñoz, Alba","first_name":"Alba"},{"last_name":"Romanczuk","full_name":"Romanczuk, Pawel","first_name":"Pawel"},{"last_name":"Yu","first_name":"Weimiao","full_name":"Yu, Weimiao"},{"last_name":"Bergert","full_name":"Bergert, Martin","first_name":"Martin"},{"last_name":"Ivanovitch","first_name":"Kenzo","full_name":"Ivanovitch, Kenzo"},{"first_name":"Guillame","full_name":"Salbreux, Guillame","last_name":"Salbreux"},{"full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J","last_name":"Heisenberg"},{"first_name":"Ewa","full_name":"Paluch, Ewa","last_name":"Paluch"}],"_id":"1271","quality_controlled":"1","issue":"1","language":[{"iso":"eng"}],"oa":1,"article_number":"74","pubrep_id":"695","publication":"BMC Biology","day":"02","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","doi":"10.1186/s12915-016-0294-x","title":"Steering cell migration by alternating blebs and actin-rich protrusions","acknowledged_ssus":[{"_id":"LifeSc"}],"year":"2016","department":[{"_id":"CaHe"}],"project":[{"grant_number":"HE_3231/6-1","_id":"252064B8-B435-11E9-9278-68D0E5697425","name":"Analysis of the Formation and Function of Different Cell Protusion Types During Cell Migration in Vivo"}],"acknowledgement":"We thank K. Lee, C. Norden, A. Webb, and the members of the Paluch lab for\r\ncomments on the manuscript. We are grateful to P. Rørth and Peter Dieterich\r\nfor discussions, S. Ares, Y. Arboleda-Estudillo and S. Schneider for technical help,\r\nM. Biro for help with programming, and the BIOTEC/MPI-CBG and IST zebrafish\r\nand imaging facilities for help and advice at various stages of this project. This work was supported by the Max Planck Society, the Medical Research Council UK (core funding to the MRC LMCB), and by grants from the Polish Ministry of Science and Higher Education (454/N-MPG/2009/0) to EKP, the Deutsche Forschungsgemeinschaft (HE 3231/6-1 and PA 1590/1-1) to CPH and EKP, a A*Star JCO career development award (12302FG010) to WY and a Damon Runyon fellowship award to ADM (DRG 2157-12). This work was also supported by the Francis Crick Institute which receives its core funding from Cancer Research UK (FC001317), the UK Medical Research Council (FC001317), and the Wellcome Trust (FC001317) to GS."},{"volume":117,"author":[{"last_name":"Callan Jones","full_name":"Callan Jones, Andrew","first_name":"Andrew"},{"last_name":"Ruprecht","full_name":"Ruprecht, Verena","first_name":"Verena","id":"4D71A03A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4088-8633"},{"orcid":"0000-0002-2670-2217","full_name":"Wieser, Stefan","first_name":"Stefan","id":"355AA5A0-F248-11E8-B48F-1D18A9856A87","last_name":"Wieser"},{"last_name":"Heisenberg","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J"},{"last_name":"Voituriez","first_name":"Raphaël","full_name":"Voituriez, Raphaël"}],"date_published":"2016-09-22T00:00:00Z","intvolume":"       117","department":[{"_id":"CaHe"}],"year":"2016","_id":"1275","quality_controlled":"1","issue":"13","title":"Callan-Jones et al. Reply","date_created":"2018-12-11T11:51:05Z","publication_status":"published","scopus_import":1,"day":"22","publication":"Physical Review Letters","month":"09","status":"public","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","publist_id":"6041","oa_version":"None","date_updated":"2021-01-12T06:49:33Z","doi":"10.1103/PhysRevLett.117.139802","language":[{"iso":"eng"}],"publisher":"American Physical Society","citation":{"apa":"Callan Jones, A., Ruprecht, V., Wieser, S., Heisenberg, C.-P. J., &#38; Voituriez, R. (2016). Callan-Jones et al. Reply. <i>Physical Review Letters</i>. American Physical Society. <a href=\"https://doi.org/10.1103/PhysRevLett.117.139802\">https://doi.org/10.1103/PhysRevLett.117.139802</a>","ieee":"A. Callan Jones, V. Ruprecht, S. Wieser, C.-P. J. Heisenberg, and R. Voituriez, “Callan-Jones et al. Reply,” <i>Physical Review Letters</i>, vol. 117, no. 13. American Physical Society, 2016.","chicago":"Callan Jones, Andrew, Verena Ruprecht, Stefan Wieser, Carl-Philipp J Heisenberg, and Raphaël Voituriez. “Callan-Jones et Al. Reply.” <i>Physical Review Letters</i>. American Physical Society, 2016. <a href=\"https://doi.org/10.1103/PhysRevLett.117.139802\">https://doi.org/10.1103/PhysRevLett.117.139802</a>.","mla":"Callan Jones, Andrew, et al. “Callan-Jones et Al. Reply.” <i>Physical Review Letters</i>, vol. 117, no. 13, 139802, American Physical Society, 2016, doi:<a href=\"https://doi.org/10.1103/PhysRevLett.117.139802\">10.1103/PhysRevLett.117.139802</a>.","short":"A. Callan Jones, V. Ruprecht, S. Wieser, C.-P.J. Heisenberg, R. Voituriez, Physical Review Letters 117 (2016).","ama":"Callan Jones A, Ruprecht V, Wieser S, Heisenberg C-PJ, Voituriez R. Callan-Jones et al. Reply. <i>Physical Review Letters</i>. 2016;117(13). doi:<a href=\"https://doi.org/10.1103/PhysRevLett.117.139802\">10.1103/PhysRevLett.117.139802</a>","ista":"Callan Jones A, Ruprecht V, Wieser S, Heisenberg C-PJ, Voituriez R. 2016. Callan-Jones et al. Reply. Physical Review Letters. 117(13), 139802."},"type":"journal_article","article_number":"139802"},{"title":"Actin rings of power","date_created":"2018-12-11T11:50:07Z","publication_status":"published","scopus_import":1,"page":"493 - 506","author":[{"last_name":"Schwayer","id":"3436488C-F248-11E8-B48F-1D18A9856A87","first_name":"Cornelia","full_name":"Schwayer, Cornelia","orcid":"0000-0001-5130-2226"},{"id":"2F74BCDE-F248-11E8-B48F-1D18A9856A87","first_name":"Mateusz K","full_name":"Sikora, Mateusz K","last_name":"Sikora"},{"id":"30F3F2F0-F248-11E8-B48F-1D18A9856A87","full_name":"Slovakova, Jana","first_name":"Jana","last_name":"Slovakova"},{"last_name":"Kardos","id":"4039350E-F248-11E8-B48F-1D18A9856A87","full_name":"Kardos, Roland","first_name":"Roland"},{"orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg"}],"volume":37,"intvolume":"        37","date_published":"2016-06-20T00:00:00Z","department":[{"_id":"CaHe"}],"year":"2016","_id":"1096","quality_controlled":"1","issue":"6","publisher":"Cell Press","language":[{"iso":"eng"}],"citation":{"chicago":"Schwayer, Cornelia, Mateusz K Sikora, Jana Slovakova, Roland Kardos, and Carl-Philipp J Heisenberg. “Actin Rings of Power.” <i>Developmental Cell</i>. Cell Press, 2016. <a href=\"https://doi.org/10.1016/j.devcel.2016.05.024\">https://doi.org/10.1016/j.devcel.2016.05.024</a>.","ieee":"C. Schwayer, M. K. Sikora, J. Slovakova, R. Kardos, and C.-P. J. Heisenberg, “Actin rings of power,” <i>Developmental Cell</i>, vol. 37, no. 6. Cell Press, pp. 493–506, 2016.","apa":"Schwayer, C., Sikora, M. K., Slovakova, J., Kardos, R., &#38; Heisenberg, C.-P. J. (2016). Actin rings of power. <i>Developmental Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.devcel.2016.05.024\">https://doi.org/10.1016/j.devcel.2016.05.024</a>","ama":"Schwayer C, Sikora MK, Slovakova J, Kardos R, Heisenberg C-PJ. Actin rings of power. <i>Developmental Cell</i>. 2016;37(6):493-506. doi:<a href=\"https://doi.org/10.1016/j.devcel.2016.05.024\">10.1016/j.devcel.2016.05.024</a>","ista":"Schwayer C, Sikora MK, Slovakova J, Kardos R, Heisenberg C-PJ. 2016. Actin rings of power. Developmental Cell. 37(6), 493–506.","short":"C. Schwayer, M.K. Sikora, J. Slovakova, R. Kardos, C.-P.J. Heisenberg, Developmental Cell 37 (2016) 493–506.","mla":"Schwayer, Cornelia, et al. “Actin Rings of Power.” <i>Developmental Cell</i>, vol. 37, no. 6, Cell Press, 2016, pp. 493–506, doi:<a href=\"https://doi.org/10.1016/j.devcel.2016.05.024\">10.1016/j.devcel.2016.05.024</a>."},"type":"journal_article","related_material":{"record":[{"status":"public","relation":"part_of_dissertation","id":"7186"}]},"day":"20","publication":"Developmental Cell","month":"06","status":"public","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","oa_version":"None","publist_id":"6279","date_updated":"2023-09-07T12:56:41Z","doi":"10.1016/j.devcel.2016.05.024"},{"ddc":["570","576"],"month":"07","status":"public","date_updated":"2024-03-25T23:30:13Z","publist_id":"6275","oa_version":"Published Version","related_material":{"record":[{"status":"public","id":"961","relation":"dissertation_contains"},{"status":"public","id":"50","relation":"dissertation_contains"}]},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"file":[{"access_level":"open_access","date_created":"2018-12-12T10:11:04Z","creator":"system","file_id":"4857","relation":"main_file","date_updated":"2018-12-12T10:11:04Z","content_type":"application/pdf","file_size":3921947,"file_name":"IST-2017-754-v1+1_1-s2.0-S2211124716307768-main.pdf"}],"type":"journal_article","citation":{"apa":"Sako, K., Pradhan, S., Barone, V., Inglés Prieto, Á., Mueller, P., Ruprecht, V., … Heisenberg, C.-P. J. (2016). Optogenetic control of nodal signaling reveals a temporal pattern of nodal signaling regulating cell fate specification during gastrulation. <i>Cell Reports</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.celrep.2016.06.036\">https://doi.org/10.1016/j.celrep.2016.06.036</a>","ieee":"K. Sako <i>et al.</i>, “Optogenetic control of nodal signaling reveals a temporal pattern of nodal signaling regulating cell fate specification during gastrulation,” <i>Cell Reports</i>, vol. 16, no. 3. Cell Press, pp. 866–877, 2016.","chicago":"Sako, Keisuke, Saurabh Pradhan, Vanessa Barone, Álvaro Inglés Prieto, Patrick Mueller, Verena Ruprecht, Daniel Capek, Sanjeev Galande, Harald L Janovjak, and Carl-Philipp J Heisenberg. “Optogenetic Control of Nodal Signaling Reveals a Temporal Pattern of Nodal Signaling Regulating Cell Fate Specification during Gastrulation.” <i>Cell Reports</i>. Cell Press, 2016. <a href=\"https://doi.org/10.1016/j.celrep.2016.06.036\">https://doi.org/10.1016/j.celrep.2016.06.036</a>.","mla":"Sako, Keisuke, et al. “Optogenetic Control of Nodal Signaling Reveals a Temporal Pattern of Nodal Signaling Regulating Cell Fate Specification during Gastrulation.” <i>Cell Reports</i>, vol. 16, no. 3, Cell Press, 2016, pp. 866–77, doi:<a href=\"https://doi.org/10.1016/j.celrep.2016.06.036\">10.1016/j.celrep.2016.06.036</a>.","short":"K. Sako, S. Pradhan, V. Barone, Á. Inglés Prieto, P. Mueller, V. Ruprecht, D. Capek, S. Galande, H.L. Janovjak, C.-P.J. Heisenberg, Cell Reports 16 (2016) 866–877.","ista":"Sako K, Pradhan S, Barone V, Inglés Prieto Á, Mueller P, Ruprecht V, Capek D, Galande S, Janovjak HL, Heisenberg C-PJ. 2016. Optogenetic control of nodal signaling reveals a temporal pattern of nodal signaling regulating cell fate specification during gastrulation. Cell Reports. 16(3), 866–877.","ama":"Sako K, Pradhan S, Barone V, et al. Optogenetic control of nodal signaling reveals a temporal pattern of nodal signaling regulating cell fate specification during gastrulation. <i>Cell Reports</i>. 2016;16(3):866-877. doi:<a href=\"https://doi.org/10.1016/j.celrep.2016.06.036\">10.1016/j.celrep.2016.06.036</a>"},"has_accepted_license":"1","publisher":"Cell Press","_id":"1100","quality_controlled":"1","issue":"3","intvolume":"        16","date_published":"2016-07-19T00:00:00Z","author":[{"last_name":"Sako","id":"3BED66BE-F248-11E8-B48F-1D18A9856A87","first_name":"Keisuke","full_name":"Sako, Keisuke","orcid":"0000-0002-6453-8075"},{"full_name":"Pradhan, Saurabh","first_name":"Saurabh","last_name":"Pradhan"},{"id":"419EECCC-F248-11E8-B48F-1D18A9856A87","full_name":"Barone, Vanessa","orcid":"0000-0003-2676-3367","first_name":"Vanessa","last_name":"Barone"},{"last_name":"Inglés Prieto","full_name":"Inglés Prieto, Álvaro","orcid":"0000-0002-5409-8571","id":"2A9DB292-F248-11E8-B48F-1D18A9856A87","first_name":"Álvaro"},{"first_name":"Patrick","full_name":"Mueller, Patrick","last_name":"Mueller"},{"id":"4D71A03A-F248-11E8-B48F-1D18A9856A87","full_name":"Ruprecht, Verena","orcid":"0000-0003-4088-8633","first_name":"Verena","last_name":"Ruprecht"},{"orcid":"0000-0001-5199-9940","full_name":"Capek, Daniel","first_name":"Daniel","id":"31C42484-F248-11E8-B48F-1D18A9856A87","last_name":"Capek"},{"last_name":"Galande","full_name":"Galande, Sanjeev","first_name":"Sanjeev"},{"last_name":"Janovjak","full_name":"Janovjak, Harald L","orcid":"0000-0002-8023-9315","id":"33BA6C30-F248-11E8-B48F-1D18A9856A87","first_name":"Harald L"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","last_name":"Heisenberg"}],"page":"866 - 877","volume":16,"scopus_import":1,"abstract":[{"text":"During metazoan development, the temporal pattern of morphogen signaling is critical for organizing cell fates in space and time. Yet, tools for temporally controlling morphogen signaling within the embryo are still scarce. Here, we developed a photoactivatable Nodal receptor to determine how the temporal pattern of Nodal signaling affects cell fate specification during zebrafish gastrulation. By using this receptor to manipulate the duration of Nodal signaling in vivo by light, we show that extended Nodal signaling within the organizer promotes prechordal plate specification and suppresses endoderm differentiation. Endoderm differentiation is suppressed by extended Nodal signaling inducing expression of the transcriptional repressor goosecoid (gsc) in prechordal plate progenitors, which in turn restrains Nodal signaling from upregulating the endoderm differentiation gene sox17 within these cells. Thus, optogenetic manipulation of Nodal signaling identifies a critical role of Nodal signaling duration for organizer cell fate specification during gastrulation.","lang":"eng"}],"file_date_updated":"2018-12-12T10:11:04Z","publication_status":"published","date_created":"2018-12-11T11:50:08Z","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","doi":"10.1016/j.celrep.2016.06.036","pubrep_id":"754","publication":"Cell Reports","day":"19","ec_funded":1,"language":[{"iso":"eng"}],"oa":1,"year":"2016","department":[{"_id":"CaHe"},{"_id":"HaJa"}],"project":[{"call_identifier":"FWF","name":"Cell- and Tissue Mechanics in Zebrafish Germ Layer Formation","_id":"2529486C-B435-11E9-9278-68D0E5697425","grant_number":"T 560-B17"},{"grant_number":"I 812-B12","name":"Cell Cortex and Germ Layer Formation in Zebrafish Gastrulation","_id":"2527D5CC-B435-11E9-9278-68D0E5697425","call_identifier":"FWF"},{"call_identifier":"FP7","grant_number":"303564","_id":"25548C20-B435-11E9-9278-68D0E5697425","name":"Microbial Ion Channels for Synthetic Neurobiology"}],"acknowledgement":"We are grateful to members of the C.-P.H. and H.J. labs for discussions, R. Hauschild and the different Scientific Service Units at IST Austria for technical help, M. Dravecka for performing initial experiments, A. Schier for reading an earlier version of the manuscript, K.W. Rogers for technical help, and C. Hill, A. Bruce, and L. Solnica-Krezel for sending plasmids. This work was supported by grants from the Austrian Science Foundation (FWF): (T560-B17) and (I 812-B12) to V.R. and C.-P.H., and from the European Union (EU FP7): (6275) to H.J. A.I.-P. is supported by a Ramon Areces fellowship.","acknowledged_ssus":[{"_id":"SSU"}],"title":"Optogenetic control of nodal signaling reveals a temporal pattern of nodal signaling regulating cell fate specification during gastrulation"},{"date_published":"2015-12-01T00:00:00Z","intvolume":"        25","page":"1423 - 1430","author":[{"last_name":"Engel","first_name":"Jakob","full_name":"Engel, Jakob"},{"first_name":"Philipp S","orcid":"0000-0002-5795-0133","id":"309D50DA-F248-11E8-B48F-1D18A9856A87","full_name":"Schmalhorst, Philipp S","last_name":"Schmalhorst"},{"last_name":"Kruger","first_name":"Anke","full_name":"Kruger, Anke"},{"last_name":"Muller","first_name":"Christina","full_name":"Muller, Christina"},{"last_name":"Buettner","full_name":"Buettner, Falk","first_name":"Falk"},{"last_name":"Routier","first_name":"Françoise","full_name":"Routier, Françoise"}],"volume":25,"pmid":1,"quality_controlled":"1","issue":"12","_id":"802","year":"2015","department":[{"_id":"CaHe"}],"publication_status":"published","external_id":{"pmid":["26306635"]},"date_created":"2018-12-11T11:48:35Z","title":"Characterization of an N-acetylglucosaminyltransferase involved in Aspergillus fumigatus zwitterionic glycoinositolphosphoceramide biosynthesis","abstract":[{"text":"Glycoinositolphosphoceramides (GIPCs) are complex sphingolipids present at the plasma membrane of various eukaryotes with the important exception of mammals. In fungi, these glycosphingolipids commonly contain an alpha-mannose residue (Man) linked at position 2 of the inositol. However, several pathogenic fungi additionally synthesize zwitterionic GIPCs carrying an alpha-glucosamine residue (GlcN) at this position. In the human pathogen Aspergillus fumigatus, the GlcNalpha1,2IPC core (where IPC is inositolphosphoceramide) is elongated to Manalpha1,3Manalpha1,6GlcNalpha1,2IPC, which is the most abundant GIPC synthesized by this fungus. In this study, we identified an A. fumigatus N-acetylglucosaminyltransferase, named GntA, and demonstrate its involvement in the initiation of zwitterionic GIPC biosynthesis. Targeted deletion of the gene encoding GntA in A. fumigatus resulted in complete absence of zwitterionic GIPC; a phenotype that could be reverted by episomal expression of GntA in the mutant. The N-acetylhexosaminyltransferase activity of GntA was substantiated by production of N-acetylhexosamine-IPC in the yeast Saccharomyces cerevisiae upon GntA expression. Using an in vitro assay, GntA was furthermore shown to use UDP-N-acetylglucosamine as donor substrate to generate a glycolipid product resistant to saponification and to digestion by phosphatidylinositol-phospholipase C as expected for GlcNAcalpha1,2IPC. Finally, as the enzymes involved in mannosylation of IPC, GntA was localized to the Golgi apparatus, the site of IPC synthesis.","lang":"eng"}],"scopus_import":1,"publication":"Glycobiology","day":"01","doi":"10.1093/glycob/cwv059","date_updated":"2021-01-12T08:16:33Z","publist_id":"6851","oa_version":"None","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","month":"12","status":"public","citation":{"apa":"Engel, J., Schmalhorst, P. S., Kruger, A., Muller, C., Buettner, F., &#38; Routier, F. (2015). Characterization of an N-acetylglucosaminyltransferase involved in Aspergillus fumigatus zwitterionic glycoinositolphosphoceramide biosynthesis. <i>Glycobiology</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/glycob/cwv059\">https://doi.org/10.1093/glycob/cwv059</a>","ieee":"J. Engel, P. S. Schmalhorst, A. Kruger, C. Muller, F. Buettner, and F. Routier, “Characterization of an N-acetylglucosaminyltransferase involved in Aspergillus fumigatus zwitterionic glycoinositolphosphoceramide biosynthesis,” <i>Glycobiology</i>, vol. 25, no. 12. Oxford University Press, pp. 1423–1430, 2015.","chicago":"Engel, Jakob, Philipp S Schmalhorst, Anke Kruger, Christina Muller, Falk Buettner, and Françoise Routier. “Characterization of an N-Acetylglucosaminyltransferase Involved in Aspergillus Fumigatus Zwitterionic Glycoinositolphosphoceramide Biosynthesis.” <i>Glycobiology</i>. Oxford University Press, 2015. <a href=\"https://doi.org/10.1093/glycob/cwv059\">https://doi.org/10.1093/glycob/cwv059</a>.","short":"J. Engel, P.S. Schmalhorst, A. Kruger, C. Muller, F. Buettner, F. Routier, Glycobiology 25 (2015) 1423–1430.","mla":"Engel, Jakob, et al. “Characterization of an N-Acetylglucosaminyltransferase Involved in Aspergillus Fumigatus Zwitterionic Glycoinositolphosphoceramide Biosynthesis.” <i>Glycobiology</i>, vol. 25, no. 12, Oxford University Press, 2015, pp. 1423–30, doi:<a href=\"https://doi.org/10.1093/glycob/cwv059\">10.1093/glycob/cwv059</a>.","ama":"Engel J, Schmalhorst PS, Kruger A, Muller C, Buettner F, Routier F. Characterization of an N-acetylglucosaminyltransferase involved in Aspergillus fumigatus zwitterionic glycoinositolphosphoceramide biosynthesis. <i>Glycobiology</i>. 2015;25(12):1423-1430. doi:<a href=\"https://doi.org/10.1093/glycob/cwv059\">10.1093/glycob/cwv059</a>","ista":"Engel J, Schmalhorst PS, Kruger A, Muller C, Buettner F, Routier F. 2015. Characterization of an N-acetylglucosaminyltransferase involved in Aspergillus fumigatus zwitterionic glycoinositolphosphoceramide biosynthesis. Glycobiology. 25(12), 1423–1430."},"language":[{"iso":"eng"}],"publisher":"Oxford University Press","type":"journal_article"},{"title":"Cortical contractility triggers a stochastic switch to fast amoeboid cell motility","year":"2015","department":[{"_id":"CaHe"},{"_id":"MiSi"}],"project":[{"grant_number":"T 560-B17","_id":"2529486C-B435-11E9-9278-68D0E5697425","name":"Cell- and Tissue Mechanics in Zebrafish Germ Layer Formation","call_identifier":"FWF"},{"call_identifier":"FWF","grant_number":"I 812-B12","_id":"2527D5CC-B435-11E9-9278-68D0E5697425","name":"Cell Cortex and Germ Layer Formation in Zebrafish Gastrulation"}],"acknowledgement":"We would like to thank R. Hausschild and E. Papusheva for technical assistance and the service facilities at the IST Austria for continuous support. The caRhoA plasmid was a kind gift of T. Kudoh and A. Takesono. We thank M. Piel and E. Paluch for exchanging unpublished data. ","acknowledged_ssus":[{"_id":"SSU"}],"language":[{"iso":"eng"}],"oa":1,"user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","doi":"10.1016/j.cell.2015.01.008","pubrep_id":"484","publication":"Cell","day":"12","scopus_import":1,"abstract":[{"lang":"eng","text":"3D amoeboid cell migration is central to many developmental and disease-related processes such as cancer metastasis. Here, we identify a unique prototypic amoeboid cell migration mode in early zebrafish embryos, termed stable-bleb migration. Stable-bleb cells display an invariant polarized balloon-like shape with exceptional migration speed and persistence. Progenitor cells can be reversibly transformed into stable-bleb cells irrespective of their primary fate and motile characteristics by increasing myosin II activity through biochemical or mechanical stimuli. Using a combination of theory and experiments, we show that, in stable-bleb cells, cortical contractility fluctuations trigger a stochastic switch into amoeboid motility, and a positive feedback between cortical flows and gradients in contractility maintains stable-bleb cell polarization. We further show that rearward cortical flows drive stable-bleb cell migration in various adhesive and non-adhesive environments, unraveling a highly versatile amoeboid migration phenotype."}],"file_date_updated":"2020-07-14T12:45:01Z","publication_status":"published","date_created":"2018-12-11T11:52:35Z","_id":"1537","quality_controlled":"1","issue":"4","date_published":"2015-02-12T00:00:00Z","intvolume":"       160","page":"673 - 685","volume":160,"author":[{"last_name":"Ruprecht","full_name":"Ruprecht, Verena","orcid":"0000-0003-4088-8633","id":"4D71A03A-F248-11E8-B48F-1D18A9856A87","first_name":"Verena"},{"last_name":"Wieser","orcid":"0000-0002-2670-2217","full_name":"Wieser, Stefan","first_name":"Stefan","id":"355AA5A0-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Callan Jones","first_name":"Andrew","full_name":"Callan Jones, Andrew"},{"last_name":"Smutny","first_name":"Michael","id":"3FE6E4E8-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-5920-9090","full_name":"Smutny, Michael"},{"last_name":"Morita","id":"4C6E54C6-F248-11E8-B48F-1D18A9856A87","first_name":"Hitoshi","full_name":"Morita, Hitoshi"},{"last_name":"Sako","first_name":"Keisuke","full_name":"Sako, Keisuke","id":"3BED66BE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6453-8075"},{"last_name":"Barone","first_name":"Vanessa","full_name":"Barone, Vanessa","id":"419EECCC-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2676-3367"},{"last_name":"Ritsch Marte","full_name":"Ritsch Marte, Monika","first_name":"Monika"},{"full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K","last_name":"Sixt"},{"last_name":"Voituriez","first_name":"Raphaël","full_name":"Voituriez, Raphaël"},{"last_name":"Heisenberg","first_name":"Carl-Philipp J","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87"}],"type":"journal_article","citation":{"ama":"Ruprecht V, Wieser S, Callan Jones A, et al. Cortical contractility triggers a stochastic switch to fast amoeboid cell motility. <i>Cell</i>. 2015;160(4):673-685. doi:<a href=\"https://doi.org/10.1016/j.cell.2015.01.008\">10.1016/j.cell.2015.01.008</a>","ista":"Ruprecht V, Wieser S, Callan Jones A, Smutny M, Morita H, Sako K, Barone V, Ritsch Marte M, Sixt MK, Voituriez R, Heisenberg C-PJ. 2015. Cortical contractility triggers a stochastic switch to fast amoeboid cell motility. Cell. 160(4), 673–685.","short":"V. Ruprecht, S. Wieser, A. Callan Jones, M. Smutny, H. Morita, K. Sako, V. Barone, M. Ritsch Marte, M.K. Sixt, R. Voituriez, C.-P.J. Heisenberg, Cell 160 (2015) 673–685.","mla":"Ruprecht, Verena, et al. “Cortical Contractility Triggers a Stochastic Switch to Fast Amoeboid Cell Motility.” <i>Cell</i>, vol. 160, no. 4, Cell Press, 2015, pp. 673–85, doi:<a href=\"https://doi.org/10.1016/j.cell.2015.01.008\">10.1016/j.cell.2015.01.008</a>.","chicago":"Ruprecht, Verena, Stefan Wieser, Andrew Callan Jones, Michael Smutny, Hitoshi Morita, Keisuke Sako, Vanessa Barone, et al. “Cortical Contractility Triggers a Stochastic Switch to Fast Amoeboid Cell Motility.” <i>Cell</i>. Cell Press, 2015. <a href=\"https://doi.org/10.1016/j.cell.2015.01.008\">https://doi.org/10.1016/j.cell.2015.01.008</a>.","apa":"Ruprecht, V., Wieser, S., Callan Jones, A., Smutny, M., Morita, H., Sako, K., … Heisenberg, C.-P. J. (2015). Cortical contractility triggers a stochastic switch to fast amoeboid cell motility. <i>Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cell.2015.01.008\">https://doi.org/10.1016/j.cell.2015.01.008</a>","ieee":"V. Ruprecht <i>et al.</i>, “Cortical contractility triggers a stochastic switch to fast amoeboid cell motility,” <i>Cell</i>, vol. 160, no. 4. Cell Press, pp. 673–685, 2015."},"publisher":"Cell Press","has_accepted_license":"1","ddc":["570"],"month":"02","status":"public","date_updated":"2023-09-07T12:05:08Z","publist_id":"5634","oa_version":"Published Version","related_material":{"record":[{"status":"public","id":"961","relation":"dissertation_contains"}]},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"file":[{"file_id":"5003","date_created":"2018-12-12T10:13:21Z","access_level":"open_access","checksum":"228d3edf40627d897b3875088a0ac51f","creator":"system","file_size":4362653,"content_type":"application/pdf","date_updated":"2020-07-14T12:45:01Z","file_name":"IST-2016-484-v1+1_1-s2.0-S0092867415000094-main.pdf","relation":"main_file"}]},{"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","month":"04","status":"public","doi":"10.1016/j.cell.2015.01.056","date_updated":"2021-01-12T06:51:33Z","publist_id":"5618","oa_version":"None","publication":"Cell","day":"09","ec_funded":1,"type":"journal_article","citation":{"apa":"Maiuri, P., Rupprecht, J., Wieser, S., Ruprecht, V., Bénichou, O., Carpi, N., … Voituriez, R. (2015). Actin flows mediate a universal coupling between cell speed and cell persistence. <i>Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cell.2015.01.056\">https://doi.org/10.1016/j.cell.2015.01.056</a>","ieee":"P. Maiuri <i>et al.</i>, “Actin flows mediate a universal coupling between cell speed and cell persistence,” <i>Cell</i>, vol. 161, no. 2. Cell Press, pp. 374–386, 2015.","chicago":"Maiuri, Paolo, Jean Rupprecht, Stefan Wieser, Verena Ruprecht, Olivier Bénichou, Nicolas Carpi, Mathieu Coppey, et al. “Actin Flows Mediate a Universal Coupling between Cell Speed and Cell Persistence.” <i>Cell</i>. Cell Press, 2015. <a href=\"https://doi.org/10.1016/j.cell.2015.01.056\">https://doi.org/10.1016/j.cell.2015.01.056</a>.","mla":"Maiuri, Paolo, et al. “Actin Flows Mediate a Universal Coupling between Cell Speed and Cell Persistence.” <i>Cell</i>, vol. 161, no. 2, Cell Press, 2015, pp. 374–86, doi:<a href=\"https://doi.org/10.1016/j.cell.2015.01.056\">10.1016/j.cell.2015.01.056</a>.","short":"P. Maiuri, J. Rupprecht, S. Wieser, V. Ruprecht, O. Bénichou, N. Carpi, M. Coppey, S. De Beco, N. Gov, C.-P.J. Heisenberg, C. Lage Crespo, F. Lautenschlaeger, M. Le Berre, A. Lennon Duménil, M. Raab, H. Thiam, M. Piel, M.K. Sixt, R. Voituriez, Cell 161 (2015) 374–386.","ama":"Maiuri P, Rupprecht J, Wieser S, et al. Actin flows mediate a universal coupling between cell speed and cell persistence. <i>Cell</i>. 2015;161(2):374-386. doi:<a href=\"https://doi.org/10.1016/j.cell.2015.01.056\">10.1016/j.cell.2015.01.056</a>","ista":"Maiuri P, Rupprecht J, Wieser S, Ruprecht V, Bénichou O, Carpi N, Coppey M, De Beco S, Gov N, Heisenberg C-PJ, Lage Crespo C, Lautenschlaeger F, Le Berre M, Lennon Duménil A, Raab M, Thiam H, Piel M, Sixt MK, Voituriez R. 2015. Actin flows mediate a universal coupling between cell speed and cell persistence. Cell. 161(2), 374–386."},"language":[{"iso":"eng"}],"publisher":"Cell Press","year":"2015","_id":"1553","department":[{"_id":"MiSi"},{"_id":"CaHe"}],"project":[{"call_identifier":"FWF","grant_number":"T 560-B17","name":"Cell- and Tissue Mechanics in Zebrafish Germ Layer Formation","_id":"2529486C-B435-11E9-9278-68D0E5697425"},{"grant_number":"281556","_id":"25A603A2-B435-11E9-9278-68D0E5697425","name":"Cytoskeletal force generation and force transduction of migrating leukocytes (EU)","call_identifier":"FP7"},{"_id":"25ABD200-B435-11E9-9278-68D0E5697425","name":"Cell migration in complex environments: from in vivo experiments to theoretical models","grant_number":"RGP0058/2011"}],"issue":"2","quality_controlled":"1","intvolume":"       161","date_published":"2015-04-09T00:00:00Z","author":[{"first_name":"Paolo","full_name":"Maiuri, Paolo","last_name":"Maiuri"},{"first_name":"Jean","full_name":"Rupprecht, Jean","last_name":"Rupprecht"},{"id":"355AA5A0-F248-11E8-B48F-1D18A9856A87","first_name":"Stefan","orcid":"0000-0002-2670-2217","full_name":"Wieser, Stefan","last_name":"Wieser"},{"first_name":"Verena","full_name":"Ruprecht, Verena","orcid":"0000-0003-4088-8633","id":"4D71A03A-F248-11E8-B48F-1D18A9856A87","last_name":"Ruprecht"},{"first_name":"Olivier","full_name":"Bénichou, Olivier","last_name":"Bénichou"},{"last_name":"Carpi","full_name":"Carpi, Nicolas","first_name":"Nicolas"},{"first_name":"Mathieu","full_name":"Coppey, Mathieu","last_name":"Coppey"},{"last_name":"De Beco","first_name":"Simon","full_name":"De Beco, Simon"},{"last_name":"Gov","first_name":"Nir","full_name":"Gov, Nir"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","last_name":"Heisenberg"},{"full_name":"Lage Crespo, Carolina","first_name":"Carolina","last_name":"Lage Crespo"},{"first_name":"Franziska","full_name":"Lautenschlaeger, Franziska","last_name":"Lautenschlaeger"},{"last_name":"Le Berre","first_name":"Maël","full_name":"Le Berre, Maël"},{"first_name":"Ana","full_name":"Lennon Duménil, Ana","last_name":"Lennon Duménil"},{"last_name":"Raab","first_name":"Matthew","full_name":"Raab, Matthew"},{"last_name":"Thiam","first_name":"Hawa","full_name":"Thiam, Hawa"},{"first_name":"Matthieu","full_name":"Piel, Matthieu","last_name":"Piel"},{"last_name":"Sixt","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K"},{"full_name":"Voituriez, Raphaël","first_name":"Raphaël","last_name":"Voituriez"}],"page":"374 - 386","volume":161,"scopus_import":1,"abstract":[{"text":"Cell movement has essential functions in development, immunity, and cancer. Various cell migration patterns have been reported, but no general rule has emerged so far. Here, we show on the basis of experimental data in vitro and in vivo that cell persistence, which quantifies the straightness of trajectories, is robustly coupled to cell migration speed. We suggest that this universal coupling constitutes a generic law of cell migration, which originates in the advection of polarity cues by an actin cytoskeleton undergoing flows at the cellular scale. Our analysis relies on a theoretical model that we validate by measuring the persistence of cells upon modulation of actin flow speeds and upon optogenetic manipulation of the binding of an actin regulator to actin filaments. Beyond the quantitative prediction of the coupling, the model yields a generic phase diagram of cellular trajectories, which recapitulates the full range of observed migration patterns.","lang":"eng"}],"title":"Actin flows mediate a universal coupling between cell speed and cell persistence","publication_status":"published","date_created":"2018-12-11T11:52:41Z"}]