---
_id: '4449'
abstract:
- lang: eng
text: Embedded software is software that interacts with physical processes. As em-
bedded systems increasingly permeate our daily lives on all levels, from micros-
copic devices to international networks, the cost-efficient development of reliable
embedded software is one of the grand challenges in computer science today. The
purpose of the workshop is to bring together researchers in all areas of computer
science that are traditionally distinct but relevant to embedded software develop-
ment, and to incubate a research community in this way. The workshop aims to cover
all aspects of the design and implementation of embedded software, inclu- ding
operating systems and middleware, programming languages and compilers, modeling
and validation, software engineering and programming methodologies, scheduling
and execution time analysis, networking and fault tolerance, as well as application
areas, such as embedded control, real-time signal processing, and telecommunications.
alternative_title:
- LNCS
article_processing_charge: No
citation:
ama: 'Henzinger TA, ed. EMSOFT: Embedded Software. Vol 2211. ACM; 2001. doi:10.1007/3-540-45449-7'
apa: 'Henzinger, T. A. (Ed.). (2001). EMSOFT: Embedded Software (Vol. 2211).
Presented at the EMSOFT 2001: Embedded Software, Tahoe City, CA, USA: ACM. https://doi.org/10.1007/3-540-45449-7'
chicago: 'Henzinger, Thomas A, ed. EMSOFT: Embedded Software. Vol. 2211.
ACM, 2001. https://doi.org/10.1007/3-540-45449-7.'
ieee: 'T. A. Henzinger, Ed., EMSOFT: Embedded Software, vol. 2211. ACM, 2001.'
ista: 'Henzinger TA ed. 2001. EMSOFT: Embedded Software, ACM,p.'
mla: 'Henzinger, Thomas A., editor. EMSOFT: Embedded Software. Vol. 2211,
ACM, 2001, doi:10.1007/3-540-45449-7.'
short: 'T.A. Henzinger, ed., EMSOFT: Embedded Software, ACM, 2001.'
conference:
end_date: 2001-10-10
location: Tahoe City, CA, USA
name: 'EMSOFT 2001: Embedded Software'
start_date: 2001-10-08
date_created: 2018-12-11T12:08:54Z
date_published: 2001-09-26T00:00:00Z
date_updated: 2023-05-10T09:53:17Z
day: '26'
doi: 10.1007/3-540-45449-7
editor:
- first_name: Thomas A
full_name: Henzinger, Thomas A
id: 40876CD8-F248-11E8-B48F-1D18A9856A87
last_name: Henzinger
orcid: 0000-0002-2985-7724
extern: '1'
intvolume: ' 2211'
language:
- iso: eng
month: '09'
oa_version: None
publication_identifier:
isbn:
- '9783540426738'
publication_status: published
publisher: ACM
publist_id: '283'
quality_controlled: '1'
status: public
title: 'EMSOFT: Embedded Software'
type: conference_editor
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 2211
year: '2001'
...
---
_id: '4475'
abstract:
- lang: eng
text: We provide an overview of the current status of HYTECH, and reflect on some
of the lessons learned from our experiences with the tool. HYTECH is a symbolic
model checker for mixed discrete-continuous systems that are modeled as automata
with piecewise-constant polyhedral differential inclusions. The use of a formal
input language and automated procedures for state-space traversal lay the foundation
for formally verifying properties of hybrid dynamical systems. We describe some
recent experiences analyzing three hybrid systems. We point out the successes
and limitations of the tool. The analysis procedure has been extended in a number
of ways to address some of the tool's shortcomings. We evaluate these extensions,
and conclude with some desiderata for verification tools for hybrid systems.
article_processing_charge: No
author:
- first_name: Thomas A
full_name: Henzinger, Thomas A
id: 40876CD8-F248-11E8-B48F-1D18A9856A87
last_name: Henzinger
orcid: 0000−0002−2985−7724
- first_name: Joerg
full_name: Preussig, Joerg
last_name: Preussig
- first_name: Howard
full_name: Wong Toi, Howard
last_name: Wong Toi
citation:
ama: 'Henzinger TA, Preussig J, Wong Toi H. Some lessons from the HYTECH experience.
In: Proceedings of the 40th IEEE Conference on Decision and Control. Vol
3. IEEE; 2001:2887-2892. doi:10.1109/.2001.980714'
apa: 'Henzinger, T. A., Preussig, J., & Wong Toi, H. (2001). Some lessons from
the HYTECH experience. In Proceedings of the 40th IEEE Conference on Decision
and Control (Vol. 3, pp. 2887–2892). Orlando, FL, USA: IEEE. https://doi.org/10.1109/.2001.980714'
chicago: Henzinger, Thomas A, Joerg Preussig, and Howard Wong Toi. “Some Lessons
from the HYTECH Experience.” In Proceedings of the 40th IEEE Conference on
Decision and Control, 3:2887–92. IEEE, 2001. https://doi.org/10.1109/.2001.980714.
ieee: T. A. Henzinger, J. Preussig, and H. Wong Toi, “Some lessons from the HYTECH
experience,” in Proceedings of the 40th IEEE Conference on Decision and Control,
Orlando, FL, USA, 2001, vol. 3, pp. 2887–2892.
ista: 'Henzinger TA, Preussig J, Wong Toi H. 2001. Some lessons from the HYTECH
experience. Proceedings of the 40th IEEE Conference on Decision and Control. CDC:
Decision and Control vol. 3, 2887–2892.'
mla: Henzinger, Thomas A., et al. “Some Lessons from the HYTECH Experience.” Proceedings
of the 40th IEEE Conference on Decision and Control, vol. 3, IEEE, 2001, pp.
2887–92, doi:10.1109/.2001.980714.
short: T.A. Henzinger, J. Preussig, H. Wong Toi, in:, Proceedings of the 40th IEEE
Conference on Decision and Control, IEEE, 2001, pp. 2887–2892.
conference:
end_date: 2001-12-07
location: Orlando, FL, USA
name: 'CDC: Decision and Control'
start_date: 2001-12-04
date_created: 2018-12-11T12:09:02Z
date_published: 2001-05-01T00:00:00Z
date_updated: 2023-05-10T09:47:20Z
day: '01'
doi: 10.1109/.2001.980714
extern: '1'
intvolume: ' 3'
language:
- iso: eng
month: '05'
oa_version: None
page: 2887 - 2892
publication: Proceedings of the 40th IEEE Conference on Decision and Control
publication_identifier:
isbn:
- '0780370619'
publication_status: published
publisher: IEEE
publist_id: '253'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Some lessons from the HYTECH experience
type: conference
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 3
year: '2001'
...
---
_id: '4477'
abstract:
- lang: eng
text: The assume-guarantee paradigm is a powerful divide-and-conquer mechanism for
decomposing a verification task about a system into subtasks about the individual
components of the system. The key to assume-guarantee reasoning is to consider
each component not in isolation, but in conjunction with assumptions about the
context of the component. Assume-guarantee principles are known for purely concurrent
contexts, which constrain the input data of a component, as well as for purely
sequential contexts, which constrain the entry configurations of a component.
We present a model for hierarchical system design which permits the arbitrary
nesting of parallel as well as serial composition, and which supports an assume-guarantee
principle for mixed parallel-serial contexts. Our model also supports both discrete
and continuous processes, and is therefore well-suited for the modeling and analysis
of embedded software systems which interact with real-world environments. Using
an example of two cooperating robots, we show refinement between a high-level
model which specifies continuous timing constraints and an implementation which
relies on discrete sampling.
acknowledgement: Support for this research was provided in part by the AFOSR MURI
grant F49620- 00-1-0327, and the DARPA SEC grant F33615-C-98-3614, the MARCO GSRC
grant 98-DT-660, the NSF ITR grant CCR-0085949.
alternative_title:
- LNCS
article_processing_charge: No
author:
- first_name: Thomas A
full_name: Henzinger, Thomas A
id: 40876CD8-F248-11E8-B48F-1D18A9856A87
last_name: Henzinger
orcid: 0000−0002−2985−7724
- first_name: Marius
full_name: Minea, Marius
last_name: Minea
- first_name: Vinayak
full_name: Prabhu, Vinayak
last_name: Prabhu
citation:
ama: 'Henzinger TA, Minea M, Prabhu V. Assume-guarantee reasoning for hierarchical
hybrid systems. In: Proceedings of the 4th International Workshop on Hybrid
Systems. Vol 2034. Springer; 2001:275-290. doi:10.1007/3-540-45351-2_24'
apa: 'Henzinger, T. A., Minea, M., & Prabhu, V. (2001). Assume-guarantee reasoning
for hierarchical hybrid systems. In Proceedings of the 4th International Workshop
on Hybrid Systems (Vol. 2034, pp. 275–290). Rome, Italy: Springer. https://doi.org/10.1007/3-540-45351-2_24'
chicago: Henzinger, Thomas A, Marius Minea, and Vinayak Prabhu. “Assume-Guarantee
Reasoning for Hierarchical Hybrid Systems.” In Proceedings of the 4th International
Workshop on Hybrid Systems, 2034:275–90. Springer, 2001. https://doi.org/10.1007/3-540-45351-2_24.
ieee: T. A. Henzinger, M. Minea, and V. Prabhu, “Assume-guarantee reasoning for
hierarchical hybrid systems,” in Proceedings of the 4th International Workshop
on Hybrid Systems, Rome, Italy, 2001, vol. 2034, pp. 275–290.
ista: 'Henzinger TA, Minea M, Prabhu V. 2001. Assume-guarantee reasoning for hierarchical
hybrid systems. Proceedings of the 4th International Workshop on Hybrid Systems.
HSCC: Hybrid Systems - Computation and Control, LNCS, vol. 2034, 275–290.'
mla: Henzinger, Thomas A., et al. “Assume-Guarantee Reasoning for Hierarchical Hybrid
Systems.” Proceedings of the 4th International Workshop on Hybrid Systems,
vol. 2034, Springer, 2001, pp. 275–90, doi:10.1007/3-540-45351-2_24.
short: T.A. Henzinger, M. Minea, V. Prabhu, in:, Proceedings of the 4th International
Workshop on Hybrid Systems, Springer, 2001, pp. 275–290.
conference:
end_date: 2001-03-30
location: Rome, Italy
name: 'HSCC: Hybrid Systems - Computation and Control'
start_date: 2001-03-28
date_created: 2018-12-11T12:09:03Z
date_published: 2001-03-14T00:00:00Z
date_updated: 2023-05-09T14:47:37Z
day: '14'
doi: 10.1007/3-540-45351-2_24
extern: '1'
intvolume: ' 2034'
language:
- iso: eng
month: '03'
oa_version: None
page: 275 - 290
publication: Proceedings of the 4th International Workshop on Hybrid Systems
publication_identifier:
isbn:
- '9783540418665'
publication_status: published
publisher: Springer
publist_id: '250'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Assume-guarantee reasoning for hierarchical hybrid systems
type: conference
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 2034
year: '2001'
...
---
_id: '4478'
abstract:
- lang: eng
text: Giotto is a principled, tool-supported design methodology for implementing
embedded control systems on platforms of possibly distributed sensors, actuators,
CPUs, and networks. Giotto is based on the principle that time-triggered task
invocations plus time-triggered mode switches can form the abstract essence of
programming real-time control systems. Giotto consists of a programming language
with a formal semantics, and a retargetable compiler and runtime library. Giotto
supports the automation of control system design by strictly separating platform-independent
functionality and timing concerns from platform-dependent scheduling and communication
issues. The time-triggered predictability of Giotto makes it particularly suitable
for safety-critical applications with hard real-time constraints. We illustrate
the platform-independence and time-triggered execution of Giotto by coordinating
a heterogeneous flock of Intel x86 robots and Lego Mindstorms robots.
acknowledgement: We thank Rupak Majumdar for implementing a prototype Giotto compiler
for Lego Mindstorms robots. We thank Dmitry Derevyanko and Winthrop Williams for
building our Intel x86 robots. We thank Edward Lee and Xiaojun Liu for help with
a Ptolemy II [4] implementation of Giotto. This research was supported in part by
the DARPA SEC grant F33615-C-98-3614, the DARPA MoBIES grant F33615- 00-C-1703,
the MARCO GSRC grant 98-DT-660, the AFOSR MURI grant F49620-00-1-0327, and the NSF
ITR grant CCR-0085949.
article_processing_charge: No
author:
- first_name: Thomas A
full_name: Henzinger, Thomas A
id: 40876CD8-F248-11E8-B48F-1D18A9856A87
last_name: Henzinger
orcid: 0000−0002−2985−7724
- first_name: Benjamin
full_name: Horowitz, Benjamin
last_name: Horowitz
- first_name: Christoph
full_name: Kirsch, Christoph
last_name: Kirsch
citation:
ama: 'Henzinger TA, Horowitz B, Kirsch C. Embedded control systems development with
Giotto. In: Proceedings of the 2nd ACM SIGPLAN Workshop on Languages, Compilers
and Tools for Embedded Systems. ACM; 2001:64-72. doi:10.1145/384197.384208'
apa: 'Henzinger, T. A., Horowitz, B., & Kirsch, C. (2001). Embedded control
systems development with Giotto. In Proceedings of the 2nd ACM SIGPLAN workshop
on Languages, compilers and tools for embedded systems (pp. 64–72). New York,
NY, United States: ACM. https://doi.org/10.1145/384197.384208'
chicago: Henzinger, Thomas A, Benjamin Horowitz, and Christoph Kirsch. “Embedded
Control Systems Development with Giotto.” In Proceedings of the 2nd ACM SIGPLAN
Workshop on Languages, Compilers and Tools for Embedded Systems, 64–72. ACM,
2001. https://doi.org/10.1145/384197.384208.
ieee: T. A. Henzinger, B. Horowitz, and C. Kirsch, “Embedded control systems development
with Giotto,” in Proceedings of the 2nd ACM SIGPLAN workshop on Languages,
compilers and tools for embedded systems, New York, NY, United States, 2001,
pp. 64–72.
ista: 'Henzinger TA, Horowitz B, Kirsch C. 2001. Embedded control systems development
with Giotto. Proceedings of the 2nd ACM SIGPLAN workshop on Languages, compilers
and tools for embedded systems. LCTES: Languages, Compilers, and Tools for Embedded
Systems, 64–72.'
mla: Henzinger, Thomas A., et al. “Embedded Control Systems Development with Giotto.”
Proceedings of the 2nd ACM SIGPLAN Workshop on Languages, Compilers and Tools
for Embedded Systems, ACM, 2001, pp. 64–72, doi:10.1145/384197.384208.
short: T.A. Henzinger, B. Horowitz, C. Kirsch, in:, Proceedings of the 2nd ACM SIGPLAN
Workshop on Languages, Compilers and Tools for Embedded Systems, ACM, 2001, pp.
64–72.
conference:
location: New York, NY, United States
name: 'LCTES: Languages, Compilers, and Tools for Embedded Systems'
date_created: 2018-12-11T12:09:03Z
date_published: 2001-06-01T00:00:00Z
date_updated: 2023-05-10T09:37:20Z
day: '01'
doi: 10.1145/384197.384208
extern: '1'
language:
- iso: eng
month: '06'
oa_version: None
page: 64 - 72
publication: Proceedings of the 2nd ACM SIGPLAN workshop on Languages, compilers and
tools for embedded systems
publication_identifier:
isbn:
- '9781581134254'
publication_status: published
publisher: ACM
publist_id: '251'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Embedded control systems development with Giotto
type: conference
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
year: '2001'
...
---
_id: '4479'
abstract:
- lang: eng
text: Giotto provides an abstract programmer’s model for the implementation of embedded
control systems with hard real-time constraints. A typical control application
consists of periodic software tasks together with a mode switching logic for enabling
and disabling tasks. Giotto specifies time-triggered sensor readings, task invocations,
and mode switches independent of any implementation platform. Giotto can be annotated
with platform constraints such as task-to-host mappings, and task and communication
schedules. The annotations are directives for the Giotto compiler, but they do
not alter the functionality and timing of a Giotto program. By separating the
platform-independent from the platform-dependent concerns, Giotto enables a great
deal of flexibility in choosing control platforms as well as a great deal of automation
in the validation and synthesis of control software. The time-triggered nature
of Giotto achieves timing predictability, which makes Giotto particularly suitable
for safety-critical applications.
acknowledgement: This research was supported in part by the DARPA SEC grant F33615-C-98-3614
and by the MARCO GSRC grant 98-DT-660.
alternative_title:
- LNCS
article_processing_charge: No
author:
- first_name: Thomas A
full_name: Henzinger, Thomas A
id: 40876CD8-F248-11E8-B48F-1D18A9856A87
last_name: Henzinger
orcid: 0000−0002−2985−7724
- first_name: Benjamin
full_name: Horowitz, Benjamin
last_name: Horowitz
- first_name: Christoph
full_name: Kirsch, Christoph
last_name: Kirsch
citation:
ama: 'Henzinger TA, Horowitz B, Kirsch C. Giotto: A time-triggered language for
embedded programming. In: Proceedings of the 1st International Workshop on
Embedded Software. Vol 2211. ACM; 2001:166-184. doi:10.1007/3-540-45449-7_12'
apa: 'Henzinger, T. A., Horowitz, B., & Kirsch, C. (2001). Giotto: A time-triggered
language for embedded programming. In Proceedings of the 1st International
Workshop on Embedded Software (Vol. 2211, pp. 166–184). Tahoe City, CA, USA:
ACM. https://doi.org/10.1007/3-540-45449-7_12'
chicago: 'Henzinger, Thomas A, Benjamin Horowitz, and Christoph Kirsch. “Giotto:
A Time-Triggered Language for Embedded Programming.” In Proceedings of the
1st International Workshop on Embedded Software, 2211:166–84. ACM, 2001. https://doi.org/10.1007/3-540-45449-7_12.'
ieee: 'T. A. Henzinger, B. Horowitz, and C. Kirsch, “Giotto: A time-triggered language
for embedded programming,” in Proceedings of the 1st International Workshop
on Embedded Software, Tahoe City, CA, USA, 2001, vol. 2211, pp. 166–184.'
ista: 'Henzinger TA, Horowitz B, Kirsch C. 2001. Giotto: A time-triggered language
for embedded programming. Proceedings of the 1st International Workshop on Embedded
Software. EMSOFT: Embedded Software , LNCS, vol. 2211, 166–184.'
mla: 'Henzinger, Thomas A., et al. “Giotto: A Time-Triggered Language for Embedded
Programming.” Proceedings of the 1st International Workshop on Embedded Software,
vol. 2211, ACM, 2001, pp. 166–84, doi:10.1007/3-540-45449-7_12.'
short: T.A. Henzinger, B. Horowitz, C. Kirsch, in:, Proceedings of the 1st International
Workshop on Embedded Software, ACM, 2001, pp. 166–184.
conference:
end_date: 2001-10-10
location: Tahoe City, CA, USA
name: 'EMSOFT: Embedded Software '
start_date: 2001-10-08
date_created: 2018-12-11T12:09:04Z
date_published: 2001-09-26T00:00:00Z
date_updated: 2023-05-10T09:42:10Z
day: '26'
doi: 10.1007/3-540-45449-7_12
extern: '1'
intvolume: ' 2211'
language:
- iso: eng
month: '09'
oa_version: None
page: 166 - 184
publication: Proceedings of the 1st International Workshop on Embedded Software
publication_identifier:
isbn:
- '9783540426738'
publication_status: published
publisher: ACM
publist_id: '252'
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Giotto: A time-triggered language for embedded programming'
type: conference
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 2211
year: '2001'
...
---
_id: '2494'
abstract:
- lang: eng
text: 'This chapter focuses on metabotropic glutamate receptors (mGluRs). These
receptors are linked to several intracellular signal transduction mechanisms via
G-protein and are implicated in diverse functions of the mammalian central nervous
system (CNS). These functions include mediation of slow excitatory and inhibitory
responses; regulation of calcium channels, potassium channels, and non-selective
cation channels; inhibition and facilitation of transmitter release; induction
of long-term potentiation and long-term depression; and regulation of neuronal
development. Functional diversity of the metabotropic glutamate receptor is reflected
in molecular diversity of receptor subtypes. The sites of action of mGluRs are
widely found throughout different membrane compartments of neuronal and glial
cells. The chapter reviews the differential subcellular localization of metabotropic
glutamate receptors in relation to transmitter release sites. Patterns of subcellular
localization of mGluRs are different among three subgroups: group I and III mGluRs
are mainly localized to somatodendritic and axonal domains of neurons, respectively,
while group II mGluRs are extensively localized to both domains as well as to
glial cell processes.'
alternative_title:
- Handbook of Chemical Neuroanatomy
article_processing_charge: No
author:
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Noboru
full_name: Mizuno, Noboru
last_name: Mizuno
citation:
ama: 'Shigemoto R, Mizuno N. Chapter III Metabotropic glutamate receptors - immunocytochemical
and in situ hybridization analyses. In: Glutamate. Vol 18. Elsevier; 2000:63-98.
doi:10.1016/S0924-8196(00)80044-5'
apa: Shigemoto, R., & Mizuno, N. (2000). Chapter III Metabotropic glutamate
receptors - immunocytochemical and in situ hybridization analyses. In Glutamate
(Vol. 18, pp. 63–98). Elsevier. https://doi.org/10.1016/S0924-8196(00)80044-5
chicago: Shigemoto, Ryuichi, and Noboru Mizuno. “Chapter III Metabotropic Glutamate
Receptors - Immunocytochemical and in Situ Hybridization Analyses.” In Glutamate,
18:63–98. Elsevier, 2000. https://doi.org/10.1016/S0924-8196(00)80044-5.
ieee: R. Shigemoto and N. Mizuno, “Chapter III Metabotropic glutamate receptors
- immunocytochemical and in situ hybridization analyses,” in Glutamate,
vol. 18, Elsevier, 2000, pp. 63–98.
ista: 'Shigemoto R, Mizuno N. 2000.Chapter III Metabotropic glutamate receptors
- immunocytochemical and in situ hybridization analyses. In: Glutamate. Handbook
of Chemical Neuroanatomy, vol. 18, 63–98.'
mla: Shigemoto, Ryuichi, and Noboru Mizuno. “Chapter III Metabotropic Glutamate
Receptors - Immunocytochemical and in Situ Hybridization Analyses.” Glutamate,
vol. 18, Elsevier, 2000, pp. 63–98, doi:10.1016/S0924-8196(00)80044-5.
short: R. Shigemoto, N. Mizuno, in:, Glutamate, Elsevier, 2000, pp. 63–98.
date_created: 2018-12-11T11:58:00Z
date_published: 2000-01-01T00:00:00Z
date_updated: 2023-05-03T12:27:40Z
day: '01'
doi: 10.1016/S0924-8196(00)80044-5
extern: '1'
intvolume: ' 18'
language:
- iso: eng
month: '01'
oa_version: None
page: 63 - 98
publication: Glutamate
publication_identifier:
eissn:
- 0924-8196
publication_status: published
publisher: Elsevier
publist_id: '4407'
quality_controlled: '1'
status: public
title: Chapter III Metabotropic glutamate receptors - immunocytochemical and in situ
hybridization analyses
type: book_chapter
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 18
year: '2000'
...
---
_id: '2598'
abstract:
- lang: eng
text: "There are many types of cerebellar ataxia, including ataxia due to congenital
or metabolic disorders and a paraneoplastic form in patients with gynecologic
cancer, breast cancer, lung cancer, or Hodgkin's disease.1 This paraneoplastic
syndrome is the only type of cerebellar ataxia associated with autoantibodies
against neuronal antigens. Often, the neuronal antigens are aberrantly expressed
by the tumor cells.2-4 The antineuronal autoantibodies are believed to cause cerebellar
ataxia, but this is unproved.5,6 In Hodgkin's disease, the lymphoma precedes the
ataxia by months to years in 80 percent of patients, and ataxia often occurs during
a prolonged complete remission.4 Among patients with this type of ataxia, 30 percent
have anti–Purkinje-cell antibodies, some of which have the features of the neuronal
antibody anti-Tr.4,7\r\n\r\nWe identified a new autoantibody in two patients with
severe cerebellar ataxia that developed while they were in remission from Hodgkin's
disease. The antibody reacts specifically with the metabotropic glutamate receptor
mGluR1 in mouse brain. Metabotropic glutamate receptors belong to a large family
of cell-surface receptors that transmit signals into the cell by coupling to guanine
nucleotide-binding proteins (G proteins) in the cytoplasm. Purified IgG from the
serum of both patients blocked the glutamate-stimulated formation of inositol
phosphates in Chinese-hamster-ovary (CHO) cells that expressed mGluR1α, and the
injection of IgG from serum or cerebrospinal fluid into the cerebellar subarachnoid
space of mice caused severe, reversible ataxia. These results indicate that antineuronal
autoantibodies can cause disease of the central nervous system by blocking neuronal
receptors."
acknowledgement: "Supported in part by the Ministry of Education, Science, and Culture
of Japan (Dr. Nakanishi and Dr. Shigemoto); CREST of Japan Science and Technology
Corporation (Dr. Shigemoto); the Life Sciences Foundation (Dr. De Zeeuw); NWO (Dr.
De Zeeuw and Dr. De Leeuw); and the Human Frontier Science Program (Dr. De Zeeuw
and Dr. Shigemoto).\r\nDrs. Sillevis Smitt and Kinoshita contributed equally to
the article. We are indebted to A. Aiba for providing mGluR1-deficient mice; to
T. Maruyama for providing human mGluR1-expressing CHO cells; to H. Jingami for helpful
discussion; to A. Uesugi for photographic assistance; to M. van den Bent and C.
Gaillard for clinical information on the patients; and to J. van der Burg, S.K.E.
Koekkoek, K.J. Reus, and C. Vermeer for technical assistance."
article_processing_charge: No
article_type: original
author:
- first_name: Peter
full_name: Sillevis Smitt, Peter
last_name: Sillevis Smitt
- first_name: Ayae
full_name: Kinoshita, Ayae
last_name: Kinoshita
- first_name: Bertie
full_name: De Leeuw, Bertie
last_name: De Leeuw
- first_name: Wiebe
full_name: Moll, Wiebe
last_name: Moll
- first_name: Michiel
full_name: Coesmans, Michiel
last_name: Coesmans
- first_name: Dick
full_name: Jaarsma, Dick
last_name: Jaarsma
- first_name: Sonja
full_name: Henzen Logmans, Sonja
last_name: Henzen Logmans
- first_name: Charles
full_name: Vecht, Charles
last_name: Vecht
- first_name: Chris
full_name: De Zeeuw, Chris
last_name: De Zeeuw
- first_name: Naotaka
full_name: Sekiyama, Naotaka
last_name: Sekiyama
- first_name: Shigetada
full_name: Nakanishi, Shigetada
last_name: Nakanishi
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
citation:
ama: Sillevis Smitt P, Kinoshita A, De Leeuw B, et al. Paraneoplastic cerebellar
ataxia due to autoantibodies against a glutamate receptor. New England Journal
of Medicine. 2000;342(1):21-27. doi:10.1056/NEJM200001063420104
apa: Sillevis Smitt, P., Kinoshita, A., De Leeuw, B., Moll, W., Coesmans, M., Jaarsma,
D., … Shigemoto, R. (2000). Paraneoplastic cerebellar ataxia due to autoantibodies
against a glutamate receptor. New England Journal of Medicine. Massachussetts
Medical Society. https://doi.org/10.1056/NEJM200001063420104
chicago: Sillevis Smitt, Peter, Ayae Kinoshita, Bertie De Leeuw, Wiebe Moll, Michiel
Coesmans, Dick Jaarsma, Sonja Henzen Logmans, et al. “Paraneoplastic Cerebellar
Ataxia Due to Autoantibodies against a Glutamate Receptor.” New England Journal
of Medicine. Massachussetts Medical Society, 2000. https://doi.org/10.1056/NEJM200001063420104.
ieee: P. Sillevis Smitt et al., “Paraneoplastic cerebellar ataxia due to
autoantibodies against a glutamate receptor,” New England Journal of Medicine,
vol. 342, no. 1. Massachussetts Medical Society, pp. 21–27, 2000.
ista: Sillevis Smitt P, Kinoshita A, De Leeuw B, Moll W, Coesmans M, Jaarsma D,
Henzen Logmans S, Vecht C, De Zeeuw C, Sekiyama N, Nakanishi S, Shigemoto R. 2000.
Paraneoplastic cerebellar ataxia due to autoantibodies against a glutamate receptor.
New England Journal of Medicine. 342(1), 21–27.
mla: Sillevis Smitt, Peter, et al. “Paraneoplastic Cerebellar Ataxia Due to Autoantibodies
against a Glutamate Receptor.” New England Journal of Medicine, vol. 342,
no. 1, Massachussetts Medical Society, 2000, pp. 21–27, doi:10.1056/NEJM200001063420104.
short: P. Sillevis Smitt, A. Kinoshita, B. De Leeuw, W. Moll, M. Coesmans, D. Jaarsma,
S. Henzen Logmans, C. Vecht, C. De Zeeuw, N. Sekiyama, S. Nakanishi, R. Shigemoto,
New England Journal of Medicine 342 (2000) 21–27.
date_created: 2018-12-11T11:58:35Z
date_published: 2000-01-06T00:00:00Z
date_updated: 2023-05-03T11:14:19Z
day: '06'
doi: 10.1056/NEJM200001063420104
extern: '1'
external_id:
pmid:
- '10620645'
intvolume: ' 342'
issue: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.nejm.org/doi/full/10.1056/nejm200001063420104
month: '01'
oa: 1
oa_version: None
page: 21 - 27
pmid: 1
publication: New England Journal of Medicine
publication_identifier:
issn:
- 0028-4793
publication_status: published
publisher: Massachussetts Medical Society
publist_id: '4300'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Paraneoplastic cerebellar ataxia due to autoantibodies against a glutamate
receptor
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 342
year: '2000'
...
---
_id: '2599'
abstract:
- lang: eng
text: 'The synaptic relationship between substance P (SP) and its receptor, i.e.,
neurokinin-1 receptor (NK1R), was examined in the striatum of the rat by confocal
laser-scanning microscopy and electron microscopy. For confocal laser-scanning
microscopy, triple-immunofluorescence histochemistry was performed to label NK1R,
SP, and vesicular acetylcholine transporter (a specific marker for cholinergic
neurons). In electron microscopic double- immunolabeling study, immunoreactivity
for NK1R was detected with the silver- intensified gold method, while immunoreactivity
for SP was detected with peroxidase immunohistochemistry. Simultaneous immunolabeling
of NK1R and SP revealed significant mismatch at the synaptic level: although some
SP- immunopositive axon terminals were in synaptic contact with NK1R- immunopositive
sites of plasma membrane, NK1R-immunoreactivity was observed at both synaptic
and non-synaptic sites of plasma membrane. Thus, SP released from the sites remote
from NK1Rs might diffuse in the extracellular fluid to act, as a paracrine neurotransmitter,
on NK1Rs distant from its releasing site. SP neurotransmission in the striatum
might occur not only synaptically but also extrasynaptically. The SP-NK1R system
might constitute an association system within the striatum.'
acknowledgement: The authors are grateful for the photographic help of Ms. Yue-Ping Yuan, Mr. Akira Uesugi, Ms. Keiko Oka-moto,
Mr. Nobuyuki Kobayashi, and Mr. Hideki Itabashi. The authors also express their
gratitudes for the support of Dr. Kajitaro Morita in Morita Clinic of Internal Medicine
and Pediatrics, Kadoma, Osaka, Japan
article_processing_charge: No
article_type: original
author:
- first_name: Jin
full_name: Li, Jin
last_name: Li
- first_name: Dan
full_name: Wang, Dan
last_name: Wang
- first_name: Takeshi
full_name: Kaneko, Takeshi
last_name: Kaneko
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Sakashi
full_name: Nomura, Sakashi
last_name: Nomura
- first_name: Noboru
full_name: Mizuno, Noboru
last_name: Mizuno
citation:
ama: 'Li J, Wang D, Kaneko T, Shigemoto R, Nomura S, Mizuno N. Relationship between
neurokinin-1 receptor and substance P in the striatum: Light and electron microscopic
immunohistochemical study in the rat. Journal of Comparative Neurology.
2000;418(2):156-163. doi:10.1002/(SICI)1096-9861(20000306)418:2<156::AID-CNE3>3.0.CO;2-Z'
apa: 'Li, J., Wang, D., Kaneko, T., Shigemoto, R., Nomura, S., & Mizuno, N.
(2000). Relationship between neurokinin-1 receptor and substance P in the striatum:
Light and electron microscopic immunohistochemical study in the rat. Journal
of Comparative Neurology. Wiley-Blackwell. https://doi.org/10.1002/(SICI)1096-9861(20000306)418:2<156::AID-CNE3>3.0.CO;2-Z'
chicago: 'Li, Jin, Dan Wang, Takeshi Kaneko, Ryuichi Shigemoto, Sakashi Nomura,
and Noboru Mizuno. “Relationship between Neurokinin-1 Receptor and Substance P
in the Striatum: Light and Electron Microscopic Immunohistochemical Study in the
Rat.” Journal of Comparative Neurology. Wiley-Blackwell, 2000. https://doi.org/10.1002/(SICI)1096-9861(20000306)418:2<156::AID-CNE3>3.0.CO;2-Z.'
ieee: 'J. Li, D. Wang, T. Kaneko, R. Shigemoto, S. Nomura, and N. Mizuno, “Relationship
between neurokinin-1 receptor and substance P in the striatum: Light and electron
microscopic immunohistochemical study in the rat,” Journal of Comparative Neurology,
vol. 418, no. 2. Wiley-Blackwell, pp. 156–163, 2000.'
ista: 'Li J, Wang D, Kaneko T, Shigemoto R, Nomura S, Mizuno N. 2000. Relationship
between neurokinin-1 receptor and substance P in the striatum: Light and electron
microscopic immunohistochemical study in the rat. Journal of Comparative Neurology.
418(2), 156–163.'
mla: 'Li, Jin, et al. “Relationship between Neurokinin-1 Receptor and Substance
P in the Striatum: Light and Electron Microscopic Immunohistochemical Study in
the Rat.” Journal of Comparative Neurology, vol. 418, no. 2, Wiley-Blackwell,
2000, pp. 156–63, doi:10.1002/(SICI)1096-9861(20000306)418:2<156::AID-CNE3>3.0.CO;2-Z.'
short: J. Li, D. Wang, T. Kaneko, R. Shigemoto, S. Nomura, N. Mizuno, Journal of
Comparative Neurology 418 (2000) 156–163.
date_created: 2018-12-11T11:58:36Z
date_published: 2000-03-06T00:00:00Z
date_updated: 2023-05-03T10:56:44Z
day: '06'
doi: 10.1002/(SICI)1096-9861(20000306)418:2<156::AID-CNE3>3.0.CO;2-Z
extern: '1'
external_id:
pmid:
- '10701441'
intvolume: ' 418'
issue: '2'
language:
- iso: eng
month: '03'
oa_version: None
page: 156 - 163
pmid: 1
publication: Journal of Comparative Neurology
publication_identifier:
issn:
- 0021-9967
publication_status: published
publisher: Wiley-Blackwell
publist_id: '4299'
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Relationship between neurokinin-1 receptor and substance P in the striatum:
Light and electron microscopic immunohistochemical study in the rat'
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 418
year: '2000'
...
---
_id: '2600'
abstract:
- lang: eng
text: 'The synaptic relationship between substance P (SP) and its receptor, i.e.
neurokinin-1 receptor (NK1R), was examined in the superficial laminae of the caudal
subnucleus of the spinal trigeminal nucleus (medullary dorsal horn; MDH) of the
rat. For confocal laser-scanning microscopy, double-immunofluorescence histochemistry
for NK1 and SP was performed. In electron microscopic double-immunolabeling study,
immunoreactivity for NK1R was detected with the silver-intensified gold method,
while immunoreactivity for SP was detected with peroxidase immunohistochemistry.
SP-immunoreactive axon terminals were observed to be in synaptic (mostly asymmetric)
contact with NK1R-immunoreactive neuronal profiles in lamina I and lamina IIo.
Although some SP-immunoreactive axon terminals were in synaptic contact with NK1R-immunoreactive
sites of plasma membranes, NK1R-immunoreactivity was observed at both synaptic
and non-synaptic sites of plasma membrane. Thus, SP released from axon terminals
might not only act on NK1Rs facing the SP-containing axon terminals, but also
diffuse in the extracellular fluid for distances larger than the synaptic cleft
to act on NK1Rs at some distances from the synaptic sites. '
article_processing_charge: No
article_type: original
author:
- first_name: Jin
full_name: Li, Jin
last_name: Li
- first_name: Dan
full_name: Wang, Dan
last_name: Wang
- first_name: Takeshi
full_name: Kaneko, Takeshi
last_name: Kaneko
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Sakashi
full_name: Nomura, Sakashi
last_name: Nomura
- first_name: Noboru
full_name: Mizuno, Noboru
last_name: Mizuno
citation:
ama: 'Li J, Wang D, Kaneko T, Shigemoto R, Nomura S, Mizuno N. The relationship
between neurokinin-1 receptor and substance P in the medullary dorsal horn: A
light and electron microscopic immunohistochemical study in the rat. Neuroscience
Research. 2000;36(4):327-334. doi:10.1016/S0168-0102(00)00095-X'
apa: 'Li, J., Wang, D., Kaneko, T., Shigemoto, R., Nomura, S., & Mizuno, N.
(2000). The relationship between neurokinin-1 receptor and substance P in the
medullary dorsal horn: A light and electron microscopic immunohistochemical study
in the rat. Neuroscience Research. Elsevier. https://doi.org/10.1016/S0168-0102(00)00095-X'
chicago: 'Li, Jin, Dan Wang, Takeshi Kaneko, Ryuichi Shigemoto, Sakashi Nomura,
and Noboru Mizuno. “The Relationship between Neurokinin-1 Receptor and Substance
P in the Medullary Dorsal Horn: A Light and Electron Microscopic Immunohistochemical
Study in the Rat.” Neuroscience Research. Elsevier, 2000. https://doi.org/10.1016/S0168-0102(00)00095-X.'
ieee: 'J. Li, D. Wang, T. Kaneko, R. Shigemoto, S. Nomura, and N. Mizuno, “The relationship
between neurokinin-1 receptor and substance P in the medullary dorsal horn: A
light and electron microscopic immunohistochemical study in the rat,” Neuroscience
Research, vol. 36, no. 4. Elsevier, pp. 327–334, 2000.'
ista: 'Li J, Wang D, Kaneko T, Shigemoto R, Nomura S, Mizuno N. 2000. The relationship
between neurokinin-1 receptor and substance P in the medullary dorsal horn: A
light and electron microscopic immunohistochemical study in the rat. Neuroscience
Research. 36(4), 327–334.'
mla: 'Li, Jin, et al. “The Relationship between Neurokinin-1 Receptor and Substance
P in the Medullary Dorsal Horn: A Light and Electron Microscopic Immunohistochemical
Study in the Rat.” Neuroscience Research, vol. 36, no. 4, Elsevier, 2000,
pp. 327–34, doi:10.1016/S0168-0102(00)00095-X.'
short: J. Li, D. Wang, T. Kaneko, R. Shigemoto, S. Nomura, N. Mizuno, Neuroscience
Research 36 (2000) 327–334.
date_created: 2018-12-11T11:58:36Z
date_published: 2000-04-01T00:00:00Z
date_updated: 2023-05-03T10:47:06Z
day: '01'
doi: 10.1016/S0168-0102(00)00095-X
extern: '1'
external_id:
pmid:
- '10771111'
intvolume: ' 36'
issue: '4'
language:
- iso: eng
month: '04'
oa_version: None
page: 327 - 334
pmid: 1
publication: Neuroscience Research
publication_identifier:
issn:
- 0168-0102
publication_status: published
publisher: Elsevier
publist_id: '4298'
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'The relationship between neurokinin-1 receptor and substance P in the medullary
dorsal horn: A light and electron microscopic immunohistochemical study in the rat'
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 36
year: '2000'
...
---
_id: '2601'
abstract:
- lang: eng
text: Targeted deletion of metabotropic glutamate receptor-subtype 1 (mGluR1) gene
can cause defects in development and function in the cerebellum. We introduced
the mGluR1α transgene into mGluR1-null mutant [mGluR1 (-/-)] mice with a Purkinje
cell (PC)-specific promoter. mGluR1-rescue mice showed normal cerebellar long-term
depression and regression of multiple climbing fiber innervation, events significantly
impaired in mGluR1 (-/-) mice. The impaired motor coordination was rescued by
this transgene, in a dose-dependent manner. We propose that mGluR1 in PCs is a
key molecule for normal synapse formation, synaptic plasticity, and motor control
in the cerebellum.
article_processing_charge: No
article_type: original
author:
- first_name: Taeko
full_name: Ichise, Taeko
last_name: Ichise
- first_name: Masanobu
full_name: Kano, Masanobu
last_name: Kano
- first_name: Kouichi
full_name: Hashimoto, Kouichi
last_name: Hashimoto
- first_name: Dai
full_name: Yanagihara, Dai
last_name: Yanagihara
- first_name: Kazuki
full_name: Nakao, Kazuki
last_name: Nakao
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Motoya
full_name: Katsuki, Motoya
last_name: Katsuki
- first_name: Atsu
full_name: Aiba, Atsu
last_name: Aiba
citation:
ama: Ichise T, Kano M, Hashimoto K, et al. mGluR1 in cerebellar Purkinje cells essential
for long-term depression, synapse elimination, and motor coordination. Science.
2000;288(5472):1832-1835. doi:10.1126/science.288.5472.1832
apa: Ichise, T., Kano, M., Hashimoto, K., Yanagihara, D., Nakao, K., Shigemoto,
R., … Aiba, A. (2000). mGluR1 in cerebellar Purkinje cells essential for long-term
depression, synapse elimination, and motor coordination. Science. American
Association for the Advancement of Science. https://doi.org/10.1126/science.288.5472.1832
chicago: Ichise, Taeko, Masanobu Kano, Kouichi Hashimoto, Dai Yanagihara, Kazuki
Nakao, Ryuichi Shigemoto, Motoya Katsuki, and Atsu Aiba. “MGluR1 in Cerebellar
Purkinje Cells Essential for Long-Term Depression, Synapse Elimination, and Motor
Coordination.” Science. American Association for the Advancement of Science,
2000. https://doi.org/10.1126/science.288.5472.1832.
ieee: T. Ichise et al., “mGluR1 in cerebellar Purkinje cells essential for
long-term depression, synapse elimination, and motor coordination,” Science,
vol. 288, no. 5472. American Association for the Advancement of Science, pp. 1832–1835,
2000.
ista: Ichise T, Kano M, Hashimoto K, Yanagihara D, Nakao K, Shigemoto R, Katsuki
M, Aiba A. 2000. mGluR1 in cerebellar Purkinje cells essential for long-term depression,
synapse elimination, and motor coordination. Science. 288(5472), 1832–1835.
mla: Ichise, Taeko, et al. “MGluR1 in Cerebellar Purkinje Cells Essential for Long-Term
Depression, Synapse Elimination, and Motor Coordination.” Science, vol.
288, no. 5472, American Association for the Advancement of Science, 2000, pp.
1832–35, doi:10.1126/science.288.5472.1832.
short: T. Ichise, M. Kano, K. Hashimoto, D. Yanagihara, K. Nakao, R. Shigemoto,
M. Katsuki, A. Aiba, Science 288 (2000) 1832–1835.
date_created: 2018-12-11T11:58:36Z
date_published: 2000-06-09T00:00:00Z
date_updated: 2023-05-03T09:53:38Z
day: '09'
doi: 10.1126/science.288.5472.1832
extern: '1'
external_id:
pmid:
- '10846166 '
intvolume: ' 288'
issue: '5472'
language:
- iso: eng
month: '06'
oa_version: None
page: 1832 - 1835
pmid: 1
publication: Science
publication_identifier:
issn:
- 0036-8075
publication_status: published
publisher: American Association for the Advancement of Science
publist_id: '4297'
quality_controlled: '1'
scopus_import: '1'
status: public
title: mGluR1 in cerebellar Purkinje cells essential for long-term depression, synapse
elimination, and motor coordination
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 288
year: '2000'
...
---
_id: '2602'
abstract:
- lang: eng
text: Although presynaptic localization of mGluR7 is well established, the mechanism
by which the receptor may control Ca2+ channels in neurons is still unknown. We
show here that cultured cerebellar granule cells express native metabotropic glutamate
receptor type 7 (mGluR7) in neuritic processes, whereas transfected mGluR7 was
also expressed in cell bodies. This allowed us to study the effect of the transfected
receptor on somatic Ca2+ channels. In transfected neurons, mGuR7 selectively inhibited
P/Q-type Ca2+ channels. The effect was mimicked by GTPγS and blocked by pertussis
toxin (PTX) or a selective antibody raised against the G-protein αo subunit, indicating
the involvement of a G(o)-like protein. The mGuR7 effect did not display the characteristics
of a direct interaction between G-protein βγ subunits and the α1A Ca2+ channel
subunit, but was abolished by quenching βγ subunits with specific intracellular
peptides. Intracellular dialysis of G-protein βγ subunits did not mimic the action
of mGluR7, suggesting that both G-protein βγ and αo subunits were required to
mediate the effect. Inhibition of phospholipase C (PLC) blocked the inhibitory
action of mGluR7, suggesting that a coincident activation of PLC by the G-protein
βγ with αo subunits was required. The Ca2+ chelator BAPTA, as well as inhibition
of either the inositol trisphosphate (IP3) receptor or protein kinase C (PKC)
abolished the mGluR7 effect. Moreover, activation of native mGluR7 induced a PTX-dependent
IP3 formation. These results indicated that IP3-mediated intracellular Ca2+ release
was required for PKC-dependent inhibition of the Ca2+ channels. Possible control
of synaptic transmission by the present mechanisms is discussed.
acknowledgement: This work was supported by Centre National de la Recherche Scientifique
and grants from Association Française contre les Myopathies, Fondation pour la Recherche
Médicale, Bayer (France), and Hoechst-Marrion-Roussel (FRHMR1/9702). We thank J.
P. Pin and F. Ango for constructive discussion of this work. We also thank Dr. J.
Saugstad (Atlanta, GA) for the rat mGluR7a cDNA, J. M. Sabatier (Marseille, France)
for the synthesis of the 68 AA peptide, V. Homburger (Montpellier, France) for the
anti-Gαo antibody, and B. Mouillac (Montpellier, France) for the anti-cMyc monoclonal
antibody.
article_processing_charge: No
article_type: original
author:
- first_name: Julie
full_name: Perroy, Julie
last_name: Perroy
- first_name: Laurent
full_name: Prezèau, Laurent
last_name: Prezèau
- first_name: Michel
full_name: De Waard, Michel
last_name: De Waard
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Joël
full_name: Bockaërt, Joël
last_name: Bockaërt
- first_name: Laurent
full_name: Fagni, Laurent
last_name: Fagni
citation:
ama: Perroy J, Prezèau L, De Waard M, Shigemoto R, Bockaërt J, Fagni L. Selective
blockade of P/Q-type calcium channels by the metabotropic glutamate receptor type
7 involves a phospholipase C pathway in neurons. Journal of Neuroscience.
2000;20(21):7896-7904. doi:10.1523/JNEUROSCI.20-21-07896.2000
apa: Perroy, J., Prezèau, L., De Waard, M., Shigemoto, R., Bockaërt, J., & Fagni,
L. (2000). Selective blockade of P/Q-type calcium channels by the metabotropic
glutamate receptor type 7 involves a phospholipase C pathway in neurons. Journal
of Neuroscience. Society for Neuroscience. https://doi.org/10.1523/JNEUROSCI.20-21-07896.2000
chicago: Perroy, Julie, Laurent Prezèau, Michel De Waard, Ryuichi Shigemoto, Joël
Bockaërt, and Laurent Fagni. “Selective Blockade of P/Q-Type Calcium Channels
by the Metabotropic Glutamate Receptor Type 7 Involves a Phospholipase C Pathway
in Neurons.” Journal of Neuroscience. Society for Neuroscience, 2000. https://doi.org/10.1523/JNEUROSCI.20-21-07896.2000.
ieee: J. Perroy, L. Prezèau, M. De Waard, R. Shigemoto, J. Bockaërt, and L. Fagni,
“Selective blockade of P/Q-type calcium channels by the metabotropic glutamate
receptor type 7 involves a phospholipase C pathway in neurons,” Journal of
Neuroscience, vol. 20, no. 21. Society for Neuroscience, pp. 7896–7904, 2000.
ista: Perroy J, Prezèau L, De Waard M, Shigemoto R, Bockaërt J, Fagni L. 2000. Selective
blockade of P/Q-type calcium channels by the metabotropic glutamate receptor type
7 involves a phospholipase C pathway in neurons. Journal of Neuroscience. 20(21),
7896–7904.
mla: Perroy, Julie, et al. “Selective Blockade of P/Q-Type Calcium Channels by the
Metabotropic Glutamate Receptor Type 7 Involves a Phospholipase C Pathway in Neurons.”
Journal of Neuroscience, vol. 20, no. 21, Society for Neuroscience, 2000,
pp. 7896–904, doi:10.1523/JNEUROSCI.20-21-07896.2000.
short: J. Perroy, L. Prezèau, M. De Waard, R. Shigemoto, J. Bockaërt, L. Fagni,
Journal of Neuroscience 20 (2000) 7896–7904.
date_created: 2018-12-11T11:58:37Z
date_published: 2000-11-01T00:00:00Z
date_updated: 2023-05-03T09:48:17Z
day: '01'
doi: 10.1523/JNEUROSCI.20-21-07896.2000
extern: '1'
external_id:
pmid:
- '11050109'
intvolume: ' 20'
issue: '21'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6772734/
month: '11'
oa: 1
oa_version: Published Version
page: 7896 - 7904
pmid: 1
publication: Journal of Neuroscience
publication_identifier:
issn:
- 0270-6474
publication_status: published
publisher: Society for Neuroscience
publist_id: '4296'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Selective blockade of P/Q-type calcium channels by the metabotropic glutamate
receptor type 7 involves a phospholipase C pathway in neurons
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 20
year: '2000'
...
---
_id: '2603'
abstract:
- lang: eng
text: Aggregation of neurotransmitter receptors at pre- and postsynaptic structures
is crucial for efficient neuronal communication. In contrast to the wealth of
information about postsynaptic specializations, little is known about the molecular
organization of presynaptic membrane proteins. We show here that the metabotropic
glutamate receptor mGluR7a, which localizes specifically to presynaptic active
zones, interacts in vitro and in vivo with PICK1. Coexpression in heterologous
systems induces coclustering dependent upon the extreme C terminus of mGluR7a
and the PDZ domain of PICK1. mGluR7a and PICK1 localize to excitatory synapses
in hippocampal neurons. Furthermore, whereas transfected mGluR7a clusters at presynaptic
sites, mGluR7aΔ3 lacking the PICK1 binding site targets to axons but does not
cluster. These results suggest that PICK1 is a component of the presynaptic machinery
involved in mGlUR7a aggregation and in modulation of glutamate neurotransmission.
acknowledgement: 'We thank Drs. S. Nakanishi, K. Moriyoshi, V. I. Gelfand, and P.
J. Conn for gifts of cDNAs and antibodies, A. S. Serpinskaya and H. Wu for excellent
preparation of neuron cultures, and H. J. Chung for help in immunoblots. This work
was supported by the Pew Chari-table Trust and National Institutes of Health grants
NS33184 and NS39286 (A. M. C.), K02MH01152 and NIDA DA10309 (P. W.), and a fellowship
from IPSEN Foundation (H. B.). '
article_processing_charge: No
article_type: original
author:
- first_name: Hélène
full_name: Boudin, Hélène
last_name: Boudin
- first_name: Andrew
full_name: Doan, Andrew
last_name: Doan
- first_name: Jun
full_name: Xia, Jun
last_name: Xia
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Richard
full_name: Huganir, Richard
last_name: Huganir
- first_name: Paul
full_name: Worley, Paul
last_name: Worley
- first_name: Ann
full_name: Craig, Ann
last_name: Craig
citation:
ama: Boudin H, Doan A, Xia J, et al. Presynaptic clustering of mGluR7a requires
the PICK1 PDZ domain binding site. Neuron. 2000;28(2):485-497. doi:10.1016/S0896-6273(00)00127-6
apa: Boudin, H., Doan, A., Xia, J., Shigemoto, R., Huganir, R., Worley, P., &
Craig, A. (2000). Presynaptic clustering of mGluR7a requires the PICK1 PDZ domain
binding site. Neuron. Elsevier. https://doi.org/10.1016/S0896-6273(00)00127-6
chicago: Boudin, Hélène, Andrew Doan, Jun Xia, Ryuichi Shigemoto, Richard Huganir,
Paul Worley, and Ann Craig. “Presynaptic Clustering of MGluR7a Requires the PICK1
PDZ Domain Binding Site.” Neuron. Elsevier, 2000. https://doi.org/10.1016/S0896-6273(00)00127-6.
ieee: H. Boudin et al., “Presynaptic clustering of mGluR7a requires the PICK1
PDZ domain binding site,” Neuron, vol. 28, no. 2. Elsevier, pp. 485–497,
2000.
ista: Boudin H, Doan A, Xia J, Shigemoto R, Huganir R, Worley P, Craig A. 2000.
Presynaptic clustering of mGluR7a requires the PICK1 PDZ domain binding site.
Neuron. 28(2), 485–497.
mla: Boudin, Hélène, et al. “Presynaptic Clustering of MGluR7a Requires the PICK1
PDZ Domain Binding Site.” Neuron, vol. 28, no. 2, Elsevier, 2000, pp. 485–97,
doi:10.1016/S0896-6273(00)00127-6.
short: H. Boudin, A. Doan, J. Xia, R. Shigemoto, R. Huganir, P. Worley, A. Craig,
Neuron 28 (2000) 485–497.
date_created: 2018-12-11T11:58:37Z
date_published: 2000-11-01T00:00:00Z
date_updated: 2023-05-03T09:41:55Z
day: '01'
doi: 10.1016/S0896-6273(00)00127-6
extern: '1'
external_id:
pmid:
- '11144358'
intvolume: ' 28'
issue: '2'
language:
- iso: eng
month: '11'
oa_version: None
page: 485 - 497
pmid: 1
publication: Neuron
publication_identifier:
issn:
- 0896-6273
publication_status: published
publisher: Elsevier
publist_id: '4295'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Presynaptic clustering of mGluR7a requires the PICK1 PDZ domain binding site
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 28
year: '2000'
...
---
_id: '2710'
abstract:
- lang: eng
text: In this paper we describe an intrinsically geometric way of producing magnetic
fields on §3 and $\R^3$ for which the corresponding Dirac operators have a non-trivial
kernel. In many cases we are able to compute the dimension of the kernel. In particular
we can give examples where the kernel has any given dimension. This generalizes
the examples of Loss and Yau (Commun. Math. Phys. 104 (1986) 283-290).
acknowledgement: L. Erdös was supported by the N.S.F. grant DMS-9970323. J. P. Solovej
was supported in parts by the EU TMR-grant FMRX-CT 96-0001 by MaPhySto — Centre
for Mathematical Physics and Stochastics, funded by a grant from The Danish National
Research Foundation and by a grant from the Danish Natural Science Research Council.
alternative_title:
- AMS/IP Studies in Advanced Mathematics
article_processing_charge: No
arxiv: 1
author:
- first_name: László
full_name: Erdös, László
id: 4DBD5372-F248-11E8-B48F-1D18A9856A87
last_name: Erdös
orcid: 0000-0001-5366-9603
citation:
ama: 'Erdös L. The kernel of Dirac operators on S3 and R3. In: Differential Equations
and Mathematical Physics. Vol 16. American Mathematical Society; 2000:111-119.
doi:10.1090/amsip/016'
apa: Erdös, L. (2000). The kernel of Dirac operators on S3 and R3. In Differential
Equations and Mathematical Physics (Vol. 16, pp. 111–119). American Mathematical
Society. https://doi.org/10.1090/amsip/016
chicago: Erdös, László. “The Kernel of Dirac Operators on S3 and R3.” In Differential
Equations and Mathematical Physics, 16:111–19. American Mathematical Society,
2000. https://doi.org/10.1090/amsip/016.
ieee: L. Erdös, “The kernel of Dirac operators on S3 and R3,” in Differential
Equations and Mathematical Physics, vol. 16, American Mathematical Society,
2000, pp. 111–119.
ista: 'Erdös L. 2000.The kernel of Dirac operators on S3 and R3. In: Differential
Equations and Mathematical Physics. AMS/IP Studies in Advanced Mathematics, vol.
16, 111–119.'
mla: Erdös, László. “The Kernel of Dirac Operators on S3 and R3.” Differential
Equations and Mathematical Physics, vol. 16, American Mathematical Society,
2000, pp. 111–19, doi:10.1090/amsip/016.
short: L. Erdös, in:, Differential Equations and Mathematical Physics, American
Mathematical Society, 2000, pp. 111–119.
date_created: 2018-12-11T11:59:12Z
date_published: 2000-01-01T00:00:00Z
date_updated: 2023-05-03T09:37:03Z
day: '01'
doi: 10.1090/amsip/016
extern: '1'
external_id:
arxiv:
- math-ph/0001036
intvolume: ' 16'
language:
- iso: eng
month: '01'
oa_version: Preprint
page: 111 - 119
publication: Differential Equations and Mathematical Physics
publication_identifier:
isbn:
- '9780821821572'
publication_status: published
publisher: American Mathematical Society
publist_id: '4186'
quality_controlled: '1'
status: public
title: The kernel of Dirac operators on S3 and R3
type: book_chapter
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 16
year: '2000'
...
---
_id: '2731'
abstract:
- lang: eng
text: We study the time evolution of a quantum particle in a Gaussian random environment.
We show that in the weak coupling limit the Wigner distribution of the wave function
converges to a solution of a linear Boltzmann equation globally in time. The Boltzmann
collision kernel is given by the Born approximation of the quantum differential
scattering cross section.
acknowledgement: Partially supported by U.S. National Science Foundation grants DMS-9403462,
9703752. We would like to thank H. Spohn for his several comments and discussions
on this project. Part of this work was done during the time when L. E. visited the
Erwin Schrödinger Institute in Vienna and when both authors visited the Center of
Theoretical Sciences in Taiwan. We thank them for the hospitality and the support
of this work.
article_processing_charge: No
article_type: original
arxiv: 1
author:
- first_name: László
full_name: Erdös, László
id: 4DBD5372-F248-11E8-B48F-1D18A9856A87
last_name: Erdös
orcid: 0000-0001-5366-9603
- first_name: Horng
full_name: Yau, Horng
last_name: Yau
citation:
ama: Erdös L, Yau H. Linear Boltzmann equation as the weak coupling limit of a random
Schrödinger equation. Communications on Pure and Applied Mathematics. 2000;53(6):667-735.
doi:10.1002/(SICI)1097-0312(200006)53:6<667::AID-CPA1>3.0.CO;2-5
apa: Erdös, L., & Yau, H. (2000). Linear Boltzmann equation as the weak coupling
limit of a random Schrödinger equation. Communications on Pure and Applied
Mathematics. Wiley-Blackwell. https://doi.org/10.1002/(SICI)1097-0312(200006)53:6<667::AID-CPA1>3.0.CO;2-5
chicago: Erdös, László, and Horng Yau. “Linear Boltzmann Equation as the Weak Coupling
Limit of a Random Schrödinger Equation.” Communications on Pure and Applied
Mathematics. Wiley-Blackwell, 2000. https://doi.org/10.1002/(SICI)1097-0312(200006)53:6<667::AID-CPA1>3.0.CO;2-5.
ieee: L. Erdös and H. Yau, “Linear Boltzmann equation as the weak coupling limit
of a random Schrödinger equation,” Communications on Pure and Applied Mathematics,
vol. 53, no. 6. Wiley-Blackwell, pp. 667–735, 2000.
ista: Erdös L, Yau H. 2000. Linear Boltzmann equation as the weak coupling limit
of a random Schrödinger equation. Communications on Pure and Applied Mathematics.
53(6), 667–735.
mla: Erdös, László, and Horng Yau. “Linear Boltzmann Equation as the Weak Coupling
Limit of a Random Schrödinger Equation.” Communications on Pure and Applied
Mathematics, vol. 53, no. 6, Wiley-Blackwell, 2000, pp. 667–735, doi:10.1002/(SICI)1097-0312(200006)53:6<667::AID-CPA1>3.0.CO;2-5.
short: L. Erdös, H. Yau, Communications on Pure and Applied Mathematics 53 (2000)
667–735.
date_created: 2018-12-11T11:59:18Z
date_published: 2000-06-01T00:00:00Z
date_updated: 2023-05-03T09:09:04Z
day: '01'
doi: 10.1002/(SICI)1097-0312(200006)53:6<667::AID-CPA1>3.0.CO;2-5
extern: '1'
external_id:
arxiv:
- math-ph/9901020
intvolume: ' 53'
issue: '6'
language:
- iso: eng
month: '06'
oa_version: Preprint
page: 667 - 735
publication: Communications on Pure and Applied Mathematics
publication_identifier:
issn:
- 0010-3640
publication_status: published
publisher: Wiley-Blackwell
publist_id: '4161'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Linear Boltzmann equation as the weak coupling limit of a random Schrödinger
equation
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 53
year: '2000'
...
---
_id: '2732'
abstract:
- lang: eng
text: 'We consider a quantum particle moving in a harmonic exterior potential and
linearly coupled to a heat bath of quantum oscillators. Caldeira and Leggett derived
the Fokker Planck equation with friction for the Wigner distribution of the particle
in the large-temperature limit: however, their (nonrigorous) derivation was not
free of criticism, especially since the limiting equation is not of Lindblad form.
In this paper we recover the correct form of their result in a rigorous way. We
also point out that the source of the diffusion is physically restrictive under
this scaling. We investigate the model at a fixed temperature and in the large-time
limit, where the origin of the diffusion is a cumulative effect of many resonant
collisions. We obtain a heat equation with a friction term for the radial process
in phase space and we prove the Einstein relation in this case.'
acknowledgement: The authors are indebted to H. Spohn for discussions. F.C. and L.E.
were partially supported by the Erwin Schrödinger Institute in Vienna (Austria)
during their visit, and they thank this institution for its hospitality. This work
was supported by the TMR-Network ``Asymptotic Methods in Kinetic Theory'' number
ERB FMBX CT97 0157 (F.C., F.F., and P.A.M.) and by NSF Grant DMS-9970323 (L.E.).
article_processing_charge: No
article_type: original
author:
- first_name: François
full_name: Castella, François
last_name: Castella
- first_name: László
full_name: Erdös, László
id: 4DBD5372-F248-11E8-B48F-1D18A9856A87
last_name: Erdös
orcid: 0000-0001-5366-9603
- first_name: Florian
full_name: Frommlet, Florian
last_name: Frommlet
- first_name: Peter
full_name: Markowich, Peter
last_name: Markowich
citation:
ama: Castella F, Erdös L, Frommlet F, Markowich P. Fokker-Planck equations as scaling
limits of reversible quantum systems. Journal of Statistical Physics. 2000;100(3-4):543-601.
doi:10.1023/A:1018667323830
apa: Castella, F., Erdös, L., Frommlet, F., & Markowich, P. (2000). Fokker-Planck
equations as scaling limits of reversible quantum systems. Journal of Statistical
Physics. Springer. https://doi.org/10.1023/A:1018667323830
chicago: Castella, François, László Erdös, Florian Frommlet, and Peter Markowich.
“Fokker-Planck Equations as Scaling Limits of Reversible Quantum Systems.” Journal
of Statistical Physics. Springer, 2000. https://doi.org/10.1023/A:1018667323830.
ieee: F. Castella, L. Erdös, F. Frommlet, and P. Markowich, “Fokker-Planck equations
as scaling limits of reversible quantum systems,” Journal of Statistical Physics,
vol. 100, no. 3–4. Springer, pp. 543–601, 2000.
ista: Castella F, Erdös L, Frommlet F, Markowich P. 2000. Fokker-Planck equations
as scaling limits of reversible quantum systems. Journal of Statistical Physics.
100(3–4), 543–601.
mla: Castella, François, et al. “Fokker-Planck Equations as Scaling Limits of Reversible
Quantum Systems.” Journal of Statistical Physics, vol. 100, no. 3–4, Springer,
2000, pp. 543–601, doi:10.1023/A:1018667323830.
short: F. Castella, L. Erdös, F. Frommlet, P. Markowich, Journal of Statistical
Physics 100 (2000) 543–601.
date_created: 2018-12-11T11:59:18Z
date_published: 2000-01-01T00:00:00Z
date_updated: 2023-05-03T09:02:11Z
day: '01'
doi: 10.1023/A:1018667323830
extern: '1'
intvolume: ' 100'
issue: 3-4
language:
- iso: eng
month: '01'
oa_version: None
page: 543 - 601
publication: Journal of Statistical Physics
publication_identifier:
issn:
- 0022-4715
publication_status: published
publisher: Springer
publist_id: '4160'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Fokker-Planck equations as scaling limits of reversible quantum systems
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 100
year: '2000'
...
---
_id: '2733'
abstract:
- lang: eng
text: The Li-Yau semiclassical lower bound for the sum of the first N eigenvalues
of the Dirichlet–Laplacian is extended to Dirichlet–Laplacians with constant magnetic
fields. Our method involves a new diamagnetic inequality for constant magnetic
fields.
article_processing_charge: No
article_type: original
author:
- first_name: László
full_name: Erdös, László
id: 4DBD5372-F248-11E8-B48F-1D18A9856A87
last_name: Erdös
orcid: 0000-0001-5366-9603
- first_name: Michael
full_name: Loss, Michael
last_name: Loss
- first_name: Vitali
full_name: Vougalter, Vitali
last_name: Vougalter
citation:
ama: Erdös L, Loss M, Vougalter V. Diamagnetic behavior of sums Dirichlet eigenvalues.
Annales de l’Institut Fourier. 2000;50(3):891-907. doi:10.5802/aif.1777
apa: Erdös, L., Loss, M., & Vougalter, V. (2000). Diamagnetic behavior of sums
Dirichlet eigenvalues. Annales de l’Institut Fourier. Association des Annales
de l’Institut Fourier. https://doi.org/10.5802/aif.1777
chicago: Erdös, László, Michael Loss, and Vitali Vougalter. “Diamagnetic Behavior
of Sums Dirichlet Eigenvalues.” Annales de l’Institut Fourier. Association
des Annales de l’Institut Fourier, 2000. https://doi.org/10.5802/aif.1777.
ieee: L. Erdös, M. Loss, and V. Vougalter, “Diamagnetic behavior of sums Dirichlet
eigenvalues,” Annales de l’Institut Fourier, vol. 50, no. 3. Association
des Annales de l’Institut Fourier, pp. 891–907, 2000.
ista: Erdös L, Loss M, Vougalter V. 2000. Diamagnetic behavior of sums Dirichlet
eigenvalues. Annales de l’Institut Fourier. 50(3), 891–907.
mla: Erdös, László, et al. “Diamagnetic Behavior of Sums Dirichlet Eigenvalues.”
Annales de l’Institut Fourier, vol. 50, no. 3, Association des Annales
de l’Institut Fourier, 2000, pp. 891–907, doi:10.5802/aif.1777.
short: L. Erdös, M. Loss, V. Vougalter, Annales de l’Institut Fourier 50 (2000)
891–907.
date_created: 2018-12-11T11:59:19Z
date_published: 2000-01-01T00:00:00Z
date_updated: 2023-05-03T08:56:17Z
day: '01'
doi: 10.5802/aif.1777
extern: '1'
intvolume: ' 50'
issue: '3'
language:
- iso: eng
month: '01'
oa_version: None
page: 891 - 907
publication: Annales de l'Institut Fourier
publication_identifier:
issn:
- 0373-0956
publication_status: published
publisher: Association des Annales de l'Institut Fourier
publist_id: '4159'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Diamagnetic behavior of sums Dirichlet eigenvalues
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 50
year: '2000'
...
---
_id: '3149'
abstract:
- lang: eng
text: The prohormone convertases (PCs) are an evolutionarily ancient group of proteases
required for the maturation of neuropeptide and peptide hormone precursors. In
Drosophila melanogaster, the homolog of prohormone convertase 2, dPC2 (amontillado),
is required for normal hatching behavior, and immunoblotting data indicate that
flies express 80- and 75-kDa forms of this protein. Because mouse PC2 (mPC2) requires
7B2, a helper protein for productive maturation, we searched the fly data base
for the 7B2 signature motif PPNPCP and identified an expressed sequence tag clone
encoding the entire open reading frame for this protein. dPC2 and d7B2 cDNAs were
subcloned into expression vectors for transfection into HEK-293 cells; mPC2 and
rat 7B2 were used as controls. Although active mPC2 was detected in medium in
the presence of either d7B2 or r7B2, dPC2 showed no proteolytic activity upon
coexpression of either d7B2 or r7B2. Labeling experiments showed that dPC2 was
synthesized but not secreted from HEK-293 cells. However, when dPC2 and either
d7B2 or r7B2 were coexpressed in Drosophila S2 cells, abundant immunoreactive
dPC2 was secreted into the medium, coincident with the appearance of PC2 activity.
Expression and secretion of dPC2 enzyme activity thus appears to require insect
cell-specific posttranslational processing events. The significant differences
in the cell biology of the insect and mammalian enzymes, with 7B2 absolutely required
for secretion of dPC2 and zymogen conversion occurring intracellularly in the
case of dPC2 but not mPC2, support the idea that the Drosophila enzyme has specific
requirements for maturation and secretion that can be met only in insect cells.
acknowledgement: This work was supported by National Institutes of Health Grants DK49703
(to I. L.), NS21749 (to P. H. T.), and GM39697 (to R. S. F.). The costs of publication
of this article were defrayed in part by the payment of page charges. This article
must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section
1734 solely to indicate this fact. 10749852. We thank members of the Lindberg laboratory
and Laurent Muller for helpful comments, Bin Tu for construction of the C. elegans
PC2 expression vector, and Joelle Finley for assistance with cell culture.
article_processing_charge: No
article_type: original
author:
- first_name: Jae
full_name: Hwang, Jae
last_name: Hwang
- first_name: Daria E
full_name: Siekhaus, Daria E
id: 3D224B9E-F248-11E8-B48F-1D18A9856A87
last_name: Siekhaus
orcid: 0000-0001-8323-8353
- first_name: Robert
full_name: Fuller, Robert
last_name: Fuller
- first_name: Paul
full_name: Taghert, Paul
last_name: Taghert
- first_name: Iris
full_name: Lindberg, Iris
last_name: Lindberg
citation:
ama: 'Hwang J, Siekhaus DE, Fuller R, Taghert P, Lindberg I. Interaction of Drosophila
melanogaster prohormone convertase 2 and 7B2: Insect cell specific processing
and secretion. Journal of Biological Chemistry. 2000;275(23):17886-17893.
doi:10.1074/jbc.M000032200 '
apa: 'Hwang, J., Siekhaus, D. E., Fuller, R., Taghert, P., & Lindberg, I. (2000).
Interaction of Drosophila melanogaster prohormone convertase 2 and 7B2: Insect
cell specific processing and secretion. Journal of Biological Chemistry.
American Society for Biochemistry and Molecular Biology. https://doi.org/10.1074/jbc.M000032200 '
chicago: 'Hwang, Jae, Daria E Siekhaus, Robert Fuller, Paul Taghert, and Iris Lindberg.
“Interaction of Drosophila Melanogaster Prohormone Convertase 2 and 7B2: Insect
Cell Specific Processing and Secretion.” Journal of Biological Chemistry.
American Society for Biochemistry and Molecular Biology, 2000. https://doi.org/10.1074/jbc.M000032200 .'
ieee: 'J. Hwang, D. E. Siekhaus, R. Fuller, P. Taghert, and I. Lindberg, “Interaction
of Drosophila melanogaster prohormone convertase 2 and 7B2: Insect cell specific
processing and secretion,” Journal of Biological Chemistry, vol. 275, no.
23. American Society for Biochemistry and Molecular Biology, pp. 17886–17893,
2000.'
ista: 'Hwang J, Siekhaus DE, Fuller R, Taghert P, Lindberg I. 2000. Interaction
of Drosophila melanogaster prohormone convertase 2 and 7B2: Insect cell specific
processing and secretion. Journal of Biological Chemistry. 275(23), 17886–17893.'
mla: 'Hwang, Jae, et al. “Interaction of Drosophila Melanogaster Prohormone Convertase
2 and 7B2: Insect Cell Specific Processing and Secretion.” Journal of Biological
Chemistry, vol. 275, no. 23, American Society for Biochemistry and Molecular
Biology, 2000, pp. 17886–93, doi:10.1074/jbc.M000032200 .'
short: J. Hwang, D.E. Siekhaus, R. Fuller, P. Taghert, I. Lindberg, Journal of Biological
Chemistry 275 (2000) 17886–17893.
date_created: 2018-12-11T12:01:40Z
date_published: 2000-06-09T00:00:00Z
date_updated: 2023-05-03T08:47:13Z
day: '09'
doi: '10.1074/jbc.M000032200 '
extern: '1'
external_id:
pmid:
- '10749852'
intvolume: ' 275'
issue: '23'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.sciencedirect.com/science/article/pii/S0021925819833215?via%3Dihub
month: '06'
oa: 1
oa_version: Published Version
page: 17886 - 17893
pmid: 1
publication: Journal of Biological Chemistry
publication_identifier:
issn:
- 0021-9258
publication_status: published
publisher: American Society for Biochemistry and Molecular Biology
publist_id: '3546'
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Interaction of Drosophila melanogaster prohormone convertase 2 and 7B2: Insect
cell specific processing and secretion'
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 275
year: '2000'
...
---
_id: '11126'
abstract:
- lang: eng
text: Nuclear import of the two uracil-rich small nuclear ribonucleoprotein (U snRNP)
components U1A and U2B′′ is mediated by unusually long and complex nuclear localization
signals (NLSs). Here we investigate nuclear import of U1A and U2B′′ in vitro and
demonstrate that it occurs by an active, saturable process. Several lines of evidence
suggest that import of the two proteins occurs by an import mechanism different
to those characterized previously. No cross competition is seen with a variety
of previously studied NLSs. In contrast to import mediated by members of the importin-β
family of nucleocytoplasmic transport receptors, U1A/U2B′′ import is not inhibited
by either nonhydrolyzable guanosine triphosphate (GTP) analogues or by a mutant
of the GTPase Ran that is incapable of GTP hydrolysis. Adenosine triphosphate
is capable of supporting U1A and U2B′′ import, whereas neither nonhydrolyzable
adenosine triphosphate analogues nor GTP can do so. U1A and U2B′′ import in vitro
does not require the addition of soluble cytosolic proteins, but a factor or factors
required for U1A and U2B′′ import remains tightly associated with the nuclear
fraction of conventionally permeabilized cells. This activity can be solubilized
in the presence of elevated MgCl2. These data suggest that U1A and U2B′′ import
into the nucleus occurs by a hitherto uncharacterized mechanism.
article_processing_charge: No
article_type: original
author:
- first_name: Martin W
full_name: HETZER, Martin W
id: 86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed
last_name: HETZER
orcid: 0000-0002-2111-992X
- first_name: Iain W.
full_name: Mattaj, Iain W.
last_name: Mattaj
citation:
ama: Hetzer M, Mattaj IW. An Atp-dependent, Ran-independent mechanism for nuclear
import of the U1a and U2b′′ spliceosome proteins. Journal of Cell Biology.
2000;148(2):293-304. doi:10.1083/jcb.148.2.293
apa: Hetzer, M., & Mattaj, I. W. (2000). An Atp-dependent, Ran-independent mechanism
for nuclear import of the U1a and U2b′′ spliceosome proteins. Journal of Cell
Biology. Rockefeller University Press. https://doi.org/10.1083/jcb.148.2.293
chicago: Hetzer, Martin, and Iain W. Mattaj. “An Atp-Dependent, Ran-Independent
Mechanism for Nuclear Import of the U1a and U2b′′ Spliceosome Proteins.” Journal
of Cell Biology. Rockefeller University Press, 2000. https://doi.org/10.1083/jcb.148.2.293.
ieee: M. Hetzer and I. W. Mattaj, “An Atp-dependent, Ran-independent mechanism for
nuclear import of the U1a and U2b′′ spliceosome proteins,” Journal of Cell
Biology, vol. 148, no. 2. Rockefeller University Press, pp. 293–304, 2000.
ista: Hetzer M, Mattaj IW. 2000. An Atp-dependent, Ran-independent mechanism for
nuclear import of the U1a and U2b′′ spliceosome proteins. Journal of Cell Biology.
148(2), 293–304.
mla: Hetzer, Martin, and Iain W. Mattaj. “An Atp-Dependent, Ran-Independent Mechanism
for Nuclear Import of the U1a and U2b′′ Spliceosome Proteins.” Journal of Cell
Biology, vol. 148, no. 2, Rockefeller University Press, 2000, pp. 293–304,
doi:10.1083/jcb.148.2.293.
short: M. Hetzer, I.W. Mattaj, Journal of Cell Biology 148 (2000) 293–304.
date_created: 2022-04-07T07:57:49Z
date_published: 2000-01-24T00:00:00Z
date_updated: 2022-07-18T08:58:29Z
day: '24'
doi: 10.1083/jcb.148.2.293
extern: '1'
external_id:
pmid:
- '10648562'
intvolume: ' 148'
issue: '2'
keyword:
- Cell Biology
language:
- iso: eng
month: '01'
oa_version: None
page: 293-304
pmid: 1
publication: Journal of Cell Biology
publication_identifier:
eissn:
- 1540-8140
issn:
- 0021-9525
publication_status: published
publisher: Rockefeller University Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: An Atp-dependent, Ran-independent mechanism for nuclear import of the U1a and
U2b′′ spliceosome proteins
type: journal_article
user_id: 72615eeb-f1f3-11ec-aa25-d4573ddc34fd
volume: 148
year: '2000'
...
---
_id: '11127'
abstract:
- lang: eng
text: Nuclear formation in Xenopus egg extracts requires cytosol and is inhibited
by GTPγS, indicating a requirement for GTPase activity. Nuclear envelope (NE)
vesicle fusion is extensively inhibited by GTPγS and two mutant forms of the Ran
GTPase, Q69L and T24N. Depletion of either Ran or RCC1, the exchange factor for
Ran, from the assembly reaction also inhibits this step of NE formation. Ran depletion
can be complemented by the addition of Ran loaded with either GTP or GDP but not
with GTPγS. RCC1 depletion is only complemented by RCC1 itself or by RanGTP. Thus,
generation of RanGTP by RCC1 and GTP hydrolysis by Ran are both required for the
extensive membrane fusion events that lead to NE formation.
article_processing_charge: No
article_type: original
author:
- first_name: Martin W
full_name: HETZER, Martin W
id: 86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed
last_name: HETZER
orcid: 0000-0002-2111-992X
- first_name: Daniel
full_name: Bilbao-Cortés, Daniel
last_name: Bilbao-Cortés
- first_name: Tobias C
full_name: Walther, Tobias C
last_name: Walther
- first_name: Oliver J
full_name: Gruss, Oliver J
last_name: Gruss
- first_name: Iain W
full_name: Mattaj, Iain W
last_name: Mattaj
citation:
ama: Hetzer M, Bilbao-Cortés D, Walther TC, Gruss OJ, Mattaj IW. GTP hydrolysis
by Ran is required for nuclear envelope assembly. Molecular Cell. 2000;5(6):1013-1024.
doi:10.1016/s1097-2765(00)80266-x
apa: Hetzer, M., Bilbao-Cortés, D., Walther, T. C., Gruss, O. J., & Mattaj,
I. W. (2000). GTP hydrolysis by Ran is required for nuclear envelope assembly.
Molecular Cell. Elsevier. https://doi.org/10.1016/s1097-2765(00)80266-x
chicago: Hetzer, Martin, Daniel Bilbao-Cortés, Tobias C Walther, Oliver J Gruss,
and Iain W Mattaj. “GTP Hydrolysis by Ran Is Required for Nuclear Envelope Assembly.”
Molecular Cell. Elsevier, 2000. https://doi.org/10.1016/s1097-2765(00)80266-x.
ieee: M. Hetzer, D. Bilbao-Cortés, T. C. Walther, O. J. Gruss, and I. W. Mattaj,
“GTP hydrolysis by Ran is required for nuclear envelope assembly,” Molecular
Cell, vol. 5, no. 6. Elsevier, pp. 1013–1024, 2000.
ista: Hetzer M, Bilbao-Cortés D, Walther TC, Gruss OJ, Mattaj IW. 2000. GTP hydrolysis
by Ran is required for nuclear envelope assembly. Molecular Cell. 5(6), 1013–1024.
mla: Hetzer, Martin, et al. “GTP Hydrolysis by Ran Is Required for Nuclear Envelope
Assembly.” Molecular Cell, vol. 5, no. 6, Elsevier, 2000, pp. 1013–24,
doi:10.1016/s1097-2765(00)80266-x.
short: M. Hetzer, D. Bilbao-Cortés, T.C. Walther, O.J. Gruss, I.W. Mattaj, Molecular
Cell 5 (2000) 1013–1024.
date_created: 2022-04-07T07:57:59Z
date_published: 2000-06-01T00:00:00Z
date_updated: 2022-07-18T08:58:31Z
day: '01'
doi: 10.1016/s1097-2765(00)80266-x
extern: '1'
external_id:
pmid:
- '10911995'
intvolume: ' 5'
issue: '6'
keyword:
- Cell Biology
- Molecular Biology
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1016/S1097-2765(00)80266-X
month: '06'
oa: 1
oa_version: Published Version
page: 1013-1024
pmid: 1
publication: Molecular Cell
publication_identifier:
issn:
- 1097-2765
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: GTP hydrolysis by Ran is required for nuclear envelope assembly
type: journal_article
user_id: 72615eeb-f1f3-11ec-aa25-d4573ddc34fd
volume: 5
year: '2000'
...
---
_id: '11683'
abstract:
- lang: eng
text: The vertex connectivity κ of a graph is the smallest number of vertices whose
deletion separates the graph or makes it trivial. We present the fastest known
deterministic algorithm for finding the vertex connectivity and a corresponding
separator. The time for a digraph having n vertices and m edges is O(min{κ3 +
n, κn}m); for an undirected graph the term m can be replaced by κn. A randomized
algorithm finds κ with error probability 1/2 in time O(nm). If the vertices have
nonnegative weights the weighted vertex connectivity is found in time O(κ1nmlog(n2/m))
where κ1 ≤ m/n is the unweighted vertex connectivity or in expected time O(nmlog(n2/m))
with error probability 1/2. The main algorithm combines two previous vertex connectivity
algorithms and a generalization of the preflow-push algorithm of Hao and Orlin
(1994, J. Algorithms17, 424–446) that computes edge connectivity.
article_processing_charge: No
article_type: original
author:
- first_name: Monika H
full_name: Henzinger, Monika H
id: 540c9bbd-f2de-11ec-812d-d04a5be85630
last_name: Henzinger
orcid: 0000-0002-5008-6530
- first_name: Satish
full_name: Rao, Satish
last_name: Rao
- first_name: Harold N.
full_name: Gabow, Harold N.
last_name: Gabow
citation:
ama: 'Henzinger MH, Rao S, Gabow HN. Computing vertex connectivity: New bounds from
old techniques. Journal of Algorithms. 2000;34(2):222-250. doi:10.1006/jagm.1999.1055'
apa: 'Henzinger, M. H., Rao, S., & Gabow, H. N. (2000). Computing vertex connectivity:
New bounds from old techniques. Journal of Algorithms. Elsevier. https://doi.org/10.1006/jagm.1999.1055'
chicago: 'Henzinger, Monika H, Satish Rao, and Harold N. Gabow. “Computing Vertex
Connectivity: New Bounds from Old Techniques.” Journal of Algorithms. Elsevier,
2000. https://doi.org/10.1006/jagm.1999.1055.'
ieee: 'M. H. Henzinger, S. Rao, and H. N. Gabow, “Computing vertex connectivity:
New bounds from old techniques,” Journal of Algorithms, vol. 34, no. 2.
Elsevier, pp. 222–250, 2000.'
ista: 'Henzinger MH, Rao S, Gabow HN. 2000. Computing vertex connectivity: New bounds
from old techniques. Journal of Algorithms. 34(2), 222–250.'
mla: 'Henzinger, Monika H., et al. “Computing Vertex Connectivity: New Bounds from
Old Techniques.” Journal of Algorithms, vol. 34, no. 2, Elsevier, 2000,
pp. 222–50, doi:10.1006/jagm.1999.1055.'
short: M.H. Henzinger, S. Rao, H.N. Gabow, Journal of Algorithms 34 (2000) 222–250.
date_created: 2022-07-28T08:56:10Z
date_published: 2000-02-01T00:00:00Z
date_updated: 2022-09-12T09:06:48Z
day: '01'
doi: 10.1006/jagm.1999.1055
extern: '1'
intvolume: ' 34'
issue: '2'
keyword:
- Computational Theory and Mathematics
- Computational Mathematics
- Control and Optimization
language:
- iso: eng
month: '02'
oa_version: None
page: 222-250
publication: Journal of Algorithms
publication_identifier:
issn:
- 0196-6774
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Computing vertex connectivity: New bounds from old techniques'
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 34
year: '2000'
...