---
_id: '2792'
abstract:
- lang: eng
text: Transition to turbulence in pipe flow has posed a riddle in fluid dynamics
since the pioneering experiments of Reynolds[1]. Although the laminar flow is
linearly stable for all flow rates, practical pipe flows become turbulent at large
enough flow speeds. Turbulence arises suddenly and fully without distinct steps
and without a clear critical point. The complexity of this problem has puzzled
mathematicians, physicists and engineers for more than a century and no satisfactory
explanation of this problem has been given. In a very recent theoretical approach
it has been suggested that unstable solutions of the Navier Stokes equations may
hold the key to understanding this problem. In numerical studies such unstable
states have been identified as exact solutions for the idealized case of a pipe
with periodic boundary conditions[2, 3]. These solutions have the form of waves
extending through the entire pipe and travelling in the streamwise direction at
a phase speed close to the bulk velocity of the fluid. With the aid of a recently
developed high-speed stereoscopic Particle Image Velocimetry (PIV) system, we
were able to observe transients of such unstable solutions in turbulent pipe flow[4].
author:
- first_name: Björn
full_name: Björn Hof
id: 3A374330-F248-11E8-B48F-1D18A9856A87
last_name: Hof
orcid: 0000-0003-2057-2754
- first_name: Casimir
full_name: van Doorne, Casimir W
last_name: Van Doorne
- first_name: Jerry
full_name: Westerweel, Jerry
last_name: Westerweel
- first_name: Frans
full_name: Nieuwstadt, Frans T
last_name: Nieuwstadt
citation:
ama: Hof B, Van Doorne C, Westerweel J, Nieuwstadt F. Observation of nonlinear travelling
waves in turbulent pipe flow. Fluid Mechanics and its Applications. 2006;78:109-114.
doi:10.1007/1-4020-4159-4_11
apa: Hof, B., Van Doorne, C., Westerweel, J., & Nieuwstadt, F. (2006). Observation
of nonlinear travelling waves in turbulent pipe flow. Fluid Mechanics and Its
Applications. Springer. https://doi.org/10.1007/1-4020-4159-4_11
chicago: Hof, Björn, Casimir Van Doorne, Jerry Westerweel, and Frans Nieuwstadt.
“Observation of Nonlinear Travelling Waves in Turbulent Pipe Flow.” Fluid Mechanics
and Its Applications. Springer, 2006. https://doi.org/10.1007/1-4020-4159-4_11.
ieee: B. Hof, C. Van Doorne, J. Westerweel, and F. Nieuwstadt, “Observation of nonlinear
travelling waves in turbulent pipe flow,” Fluid Mechanics and its Applications,
vol. 78. Springer, pp. 109–114, 2006.
ista: Hof B, Van Doorne C, Westerweel J, Nieuwstadt F. 2006. Observation of nonlinear
travelling waves in turbulent pipe flow. Fluid Mechanics and its Applications.
78, 109–114.
mla: Hof, Björn, et al. “Observation of Nonlinear Travelling Waves in Turbulent
Pipe Flow.” Fluid Mechanics and Its Applications, vol. 78, Springer, 2006,
pp. 109–14, doi:10.1007/1-4020-4159-4_11.
short: B. Hof, C. Van Doorne, J. Westerweel, F. Nieuwstadt, Fluid Mechanics and
Its Applications 78 (2006) 109–114.
date_created: 2018-12-11T11:59:37Z
date_published: 2006-01-18T00:00:00Z
date_updated: 2021-01-12T06:59:45Z
day: '18'
doi: 10.1007/1-4020-4159-4_11
extern: 1
intvolume: ' 78'
month: '01'
page: 109 - 114
publication: Fluid Mechanics and its Applications
publication_status: published
publisher: Springer
publist_id: '4097'
quality_controlled: 0
status: public
title: Observation of nonlinear travelling waves in turbulent pipe flow
type: journal_article
volume: 78
year: '2006'
...
---
_id: '2894'
abstract:
- lang: eng
text: IL-10 is a potent anti-inflammatory and immunomodulatory cytokine, exerting
major effects in the degree and quality of the immune response. Using a newly
generated IL-10 reporter mouse model, which easily allows the study of IL-10 expression
from each allele in a single cell, we report here for the first time that IL-10
is predominantly monoallelic expressed in CD4+ T cells. Furthermore, we have compelling
evidence that this expression pattern is not due to parental imprinting, allelic
exclusion, or strong allelic bias. Instead, our results support a stochastic regulation
mechanism, in which the probability to initiate allelic transcription depends
on the strength of TCR signaling and subsequent capacity to overcome restrictions
imposed by chromatin hypoacetylation. In vivo Ag-experienced T cells show a higher
basal probability to transcribe IL-10 when compared with naive cells, yet still
show mostly monoallelic IL-10 expression. Finally, statistical analysis on allelic
expression data shows transcriptional independence between both alleles. We conclude
that CD4+ T cells have a low probability for IL-10 allelic activation resulting
in a predominantly monoallelic expression pattern, and that IL-10 expression appears
to be stochastically regulated by controlling the frequency of expressing cells,
rather than absolute protein levels per cell.
author:
- first_name: Dinis
full_name: Calado, Dinis P
last_name: Calado
- first_name: Tiago
full_name: Tiago Paixao
id: 2C5658E6-F248-11E8-B48F-1D18A9856A87
last_name: Paixao
orcid: 0000-0003-2361-3953
- first_name: Dan
full_name: Holmberg, Dan
last_name: Holmberg
- first_name: Matthias
full_name: Haury, Matthias
last_name: Haury
citation:
ama: Calado D, Paixao T, Holmberg D, Haury M. Stochastic Monoallelic Expression
of IL 10 in T Cells. Journal of Immunology. 2006;177(8):5358-5364. doi:10.4049/jimmunol.177.8.5358
apa: Calado, D., Paixao, T., Holmberg, D., & Haury, M. (2006). Stochastic Monoallelic
Expression of IL 10 in T Cells. Journal of Immunology. American Association
of Immunologists. https://doi.org/10.4049/jimmunol.177.8.5358
chicago: Calado, Dinis, Tiago Paixao, Dan Holmberg, and Matthias Haury. “Stochastic
Monoallelic Expression of IL 10 in T Cells.” Journal of Immunology. American
Association of Immunologists, 2006. https://doi.org/10.4049/jimmunol.177.8.5358 .
ieee: D. Calado, T. Paixao, D. Holmberg, and M. Haury, “Stochastic Monoallelic Expression
of IL 10 in T Cells,” Journal of Immunology, vol. 177, no. 8. American
Association of Immunologists, pp. 5358–5364, 2006.
ista: Calado D, Paixao T, Holmberg D, Haury M. 2006. Stochastic Monoallelic Expression
of IL 10 in T Cells. Journal of Immunology. 177(8), 5358–5364.
mla: Calado, Dinis, et al. “Stochastic Monoallelic Expression of IL 10 in T Cells.”
Journal of Immunology, vol. 177, no. 8, American Association of Immunologists,
2006, pp. 5358–64, doi:10.4049/jimmunol.177.8.5358
.
short: D. Calado, T. Paixao, D. Holmberg, M. Haury, Journal of Immunology 177 (2006)
5358–5364.
date_created: 2018-12-11T12:00:11Z
date_published: 2006-01-01T00:00:00Z
date_updated: 2021-01-12T07:00:32Z
day: '01'
doi: '10.4049/jimmunol.177.8.5358 '
extern: 1
intvolume: ' 177'
issue: '8'
month: '01'
page: 5358 - 5364
publication: Journal of Immunology
publication_status: published
publisher: American Association of Immunologists
publist_id: '3864'
quality_controlled: 0
status: public
title: Stochastic Monoallelic Expression of IL 10 in T Cells
type: journal_article
volume: 177
year: '2006'
...
---
_id: '2921'
abstract:
- lang: eng
text: Most binocular stereo algorithms assume that all scene elements are visible
from both cameras. Scene elements that are visible from only one camera, known
as occlusions, pose an important challenge for stereo. Occlusions are important
for segmentation, because they appear near discontinuities. However, stereo algorithms
tend to ignore occlusions because of their difficulty. One reason is that occlusions
require the input images to be treated symmetrically, which complicates the problem
formulation. Worse, certain depth maps imply physically impossible scene configurations,
and must be excluded from the output. In this chapter we approach the problem
of binocular stereo with occlusions from an energy minimization viewpoint. We
begin by reviewing traditional stereo methods that do not handle occlusions. If
occlusions are ignored, it is easy to formulate the stereo problem as a pixel
labeling problem, which leads to an energy function that is common in early vision.
This kind of energy function can he minimized using graph cuts, which is a combinatorial
optimization technique that has proven to be very effective for low-level vision
problems. Motivated by this, we have designed two graph cut stereo algorithms
that are designed to handle occlusions. These algorithms produce promising experimental
results on real data with ground truth.
author:
- first_name: Vladimir
full_name: Vladimir Kolmogorov
id: 3D50B0BA-F248-11E8-B48F-1D18A9856A87
last_name: Kolmogorov
- first_name: Ramin
full_name: Zabih, Ramin
last_name: Zabih
citation:
ama: 'Kolmogorov V, Zabih R. Graph cut algorithms for binocular stereo with occlusions.
In: Handbook of Mathematical Models in Computer Vision. Springer; 2006:423-427.
doi:10.1007/0-387-28831-7_26'
apa: Kolmogorov, V., & Zabih, R. (2006). Graph cut algorithms for binocular
stereo with occlusions. In Handbook of Mathematical Models in Computer Vision
(pp. 423–427). Springer. https://doi.org/10.1007/0-387-28831-7_26
chicago: Kolmogorov, Vladimir, and Ramin Zabih. “Graph Cut Algorithms for Binocular
Stereo with Occlusions.” In Handbook of Mathematical Models in Computer Vision,
423–27. Springer, 2006. https://doi.org/10.1007/0-387-28831-7_26.
ieee: V. Kolmogorov and R. Zabih, “Graph cut algorithms for binocular stereo with
occlusions,” in Handbook of Mathematical Models in Computer Vision, Springer,
2006, pp. 423–427.
ista: 'Kolmogorov V, Zabih R. 2006.Graph cut algorithms for binocular stereo with
occlusions. In: Handbook of Mathematical Models in Computer Vision. , 423–427.'
mla: Kolmogorov, Vladimir, and Ramin Zabih. “Graph Cut Algorithms for Binocular
Stereo with Occlusions.” Handbook of Mathematical Models in Computer Vision,
Springer, 2006, pp. 423–27, doi:10.1007/0-387-28831-7_26.
short: V. Kolmogorov, R. Zabih, in:, Handbook of Mathematical Models in Computer
Vision, Springer, 2006, pp. 423–427.
date_created: 2018-12-11T12:00:21Z
date_published: 2006-01-01T00:00:00Z
date_updated: 2021-01-12T07:00:42Z
day: '01'
doi: 10.1007/0-387-28831-7_26
extern: 1
month: '01'
page: 423 - 427
publication: Handbook of Mathematical Models in Computer Vision
publication_status: published
publisher: Springer
publist_id: '3816'
quality_controlled: 0
status: public
title: Graph cut algorithms for binocular stereo with occlusions
type: book_chapter
year: '2006'
...
---
_id: '3002'
abstract:
- lang: eng
text: Arabidopsis thaliana is currently the most important model organism for basic
molecular plant research. It is also a favourable model for developmental biology,
as its embryogenesis follows a nearly invariant pattern of cell divisions and
cell type specifications. Study of embryogenesis can involve genetic, physiological
or biochemical approaches, but is always limited by the inaccessibility of the
embryos which develop deep inside maternal tissue. Thus, for developmental studies,
there is an increasing demand for methods which allow embryogenesis under artificial
conditions, providing better accessibility to experimental manipulation. In this
chapter, we address theoretical aspects of embryo culture, give some thoughts
on which embryo culture system is suited best for which application and finally
discuss three current methods which have been successfully used in Arabidopsis
embryo culture. © 2006 Springer-Verlag Berlin Heidelberg.
alternative_title:
- Plant Cell Monographs
author:
- first_name: Michael
full_name: Sauer, Michael
last_name: Sauer
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: 'Sauer M, Friml J. In vitro culture of Arabidopsis embryos. In: Mujib A, Šamaj
J, eds. Somatic Embryogenesis. Vol 2. Springer; 2006:343-354. doi:10.1007/7089_020'
apa: Sauer, M., & Friml, J. (2006). In vitro culture of Arabidopsis embryos.
In A. Mujib & J. Šamaj (Eds.), Somatic Embryogenesis (Vol. 2, pp. 343–354).
Springer. https://doi.org/10.1007/7089_020
chicago: Sauer, Michael, and Jiří Friml. “In Vitro Culture of Arabidopsis Embryos.”
In Somatic Embryogenesis, edited by Abdul Mujib and Jozef Šamaj, 2:343–54.
Springer, 2006. https://doi.org/10.1007/7089_020.
ieee: M. Sauer and J. Friml, “In vitro culture of Arabidopsis embryos,” in Somatic
Embryogenesis, vol. 2, A. Mujib and J. Šamaj, Eds. Springer, 2006, pp. 343–354.
ista: 'Sauer M, Friml J. 2006.In vitro culture of Arabidopsis embryos. In: Somatic
Embryogenesis. Plant Cell Monographs, vol. 2, 343–354.'
mla: Sauer, Michael, and Jiří Friml. “In Vitro Culture of Arabidopsis Embryos.”
Somatic Embryogenesis, edited by Abdul Mujib and Jozef Šamaj, vol. 2, Springer,
2006, pp. 343–54, doi:10.1007/7089_020.
short: M. Sauer, J. Friml, in:, A. Mujib, J. Šamaj (Eds.), Somatic Embryogenesis,
Springer, 2006, pp. 343–354.
date_created: 2018-12-11T12:00:48Z
date_published: 2006-01-01T00:00:00Z
date_updated: 2021-01-12T07:40:23Z
day: '01'
doi: 10.1007/7089_020
editor:
- first_name: Abdul
full_name: Mujib, Abdul
last_name: Mujib
- first_name: Jozef
full_name: Šamaj, Jozef
last_name: Šamaj
extern: 1
intvolume: ' 2'
month: '01'
page: 343 - 354
publication: Somatic Embryogenesis
publication_status: published
publisher: Springer
publist_id: '3699'
quality_controlled: 0
status: public
title: In vitro culture of Arabidopsis embryos
type: book_chapter
volume: 2
year: '2006'
...
---
_id: '3005'
author:
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Philip
full_name: Benfey, Philip
last_name: Benfey
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Malcolm
full_name: Bennett, Malcolm
last_name: Bennett
- first_name: Thomas
full_name: Berleth, Thomas
last_name: Berleth
- first_name: Niko
full_name: Geldner, Niko
last_name: Geldner
- first_name: Markus
full_name: Grebe, Markus
last_name: Grebe
- first_name: Marcus
full_name: Heisler, Marcus
last_name: Heisler
- first_name: Jan
full_name: Hejátko, Jan
last_name: Hejátko
- first_name: Gerd
full_name: Jürgens, Gerd
last_name: Jürgens
- first_name: Thomas
full_name: Laux, Thomas
last_name: Laux
- first_name: Keith
full_name: Lindsey, Keith
last_name: Lindsey
- first_name: Wolfgang
full_name: Lukowitz, Wolfgang
last_name: Lukowitz
- first_name: Christian
full_name: Luschnig, Christian
last_name: Luschnig
- first_name: Remko
full_name: Offringa, Remko
last_name: Offringa
- first_name: Ben
full_name: Scheres, Ben
last_name: Scheres
- first_name: Ranjan
full_name: Swarup, Ranjan
last_name: Swarup
- first_name: Ramón
full_name: Torres Ruiz, Ramón
last_name: Torres Ruiz
- first_name: Dolf
full_name: Weijers, Dolf
last_name: Weijers
- first_name: Eva
full_name: Zažímalová, Eva
last_name: Zažímalová
citation:
ama: 'Friml J, Benfey P, Benková E, et al. Apical-basal polarity: Why plant cells
don’t stand on their heads. Trends in Plant Science. 2006;11(1):12-14.
doi:10.1016/j.tplants.2005.11.010'
apa: 'Friml, J., Benfey, P., Benková, E., Bennett, M., Berleth, T., Geldner, N.,
… Zažímalová, E. (2006). Apical-basal polarity: Why plant cells don’t stand on
their heads. Trends in Plant Science. Cell Press. https://doi.org/10.1016/j.tplants.2005.11.010'
chicago: 'Friml, Jiří, Philip Benfey, Eva Benková, Malcolm Bennett, Thomas Berleth,
Niko Geldner, Markus Grebe, et al. “Apical-Basal Polarity: Why Plant Cells Don’t
Stand on Their Heads.” Trends in Plant Science. Cell Press, 2006. https://doi.org/10.1016/j.tplants.2005.11.010.'
ieee: 'J. Friml et al., “Apical-basal polarity: Why plant cells don’t stand
on their heads,” Trends in Plant Science, vol. 11, no. 1. Cell Press, pp.
12–14, 2006.'
ista: 'Friml J, Benfey P, Benková E, Bennett M, Berleth T, Geldner N, Grebe M, Heisler
M, Hejátko J, Jürgens G, Laux T, Lindsey K, Lukowitz W, Luschnig C, Offringa R,
Scheres B, Swarup R, Torres Ruiz R, Weijers D, Zažímalová E. 2006. Apical-basal
polarity: Why plant cells don’t stand on their heads. Trends in Plant Science.
11(1), 12–14.'
mla: 'Friml, Jiří, et al. “Apical-Basal Polarity: Why Plant Cells Don’t Stand on
Their Heads.” Trends in Plant Science, vol. 11, no. 1, Cell Press, 2006,
pp. 12–14, doi:10.1016/j.tplants.2005.11.010.'
short: J. Friml, P. Benfey, E. Benková, M. Bennett, T. Berleth, N. Geldner, M. Grebe,
M. Heisler, J. Hejátko, G. Jürgens, T. Laux, K. Lindsey, W. Lukowitz, C. Luschnig,
R. Offringa, B. Scheres, R. Swarup, R. Torres Ruiz, D. Weijers, E. Zažímalová,
Trends in Plant Science 11 (2006) 12–14.
date_created: 2018-12-11T12:00:49Z
date_published: 2006-01-01T00:00:00Z
date_updated: 2021-01-12T07:40:24Z
day: '01'
doi: 10.1016/j.tplants.2005.11.010
extern: '1'
intvolume: ' 11'
issue: '1'
language:
- iso: eng
month: '01'
oa_version: None
page: 12 - 14
publication: Trends in Plant Science
publication_status: published
publisher: Cell Press
publist_id: '3697'
status: public
title: 'Apical-basal polarity: Why plant cells don''t stand on their heads'
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 11
year: '2006'
...
---
_id: '3006'
abstract:
- lang: eng
text: 'Dividing plant cells perform a remarkable task of building a new cell wall
within the cytoplasm in a few minutes. A long-standing paradigm claims that this
primordial cell wall, known as the cell plate, is generated by delivery of newly
synthesized material from Golgi apparatus-originated secretory vesicles. Here,
we show that, in diverse plant species, cell surface material, including plasma
membrane proteins, cell wall components, and exogenously applied endocytic tracers,
is rapidly delivered to the forming cell plate. Importantly, this occurs even
when de novo protein synthesis is blocked. In addition, cytokinesis-specific syntaxin
KNOLLE as well as plasma membrane (PM) resident proteins localize to endosomes
that fuse to initiate the cell plate. The rate of endocytosis is strongly enhanced
during cell plate formation, and its genetic or pharmacological inhibition leads
to cytokinesis defects. Our results reveal that endocytic delivery of cell surface
material significantly contributes to cell plate formation during plant cytokinesis. '
author:
- first_name: Pankaj
full_name: Dhonukshe, Pankaj
last_name: Dhonukshe
- first_name: František
full_name: Baluška, František
last_name: Baluška
- first_name: Markus
full_name: Schlicht, Markus
last_name: Schlicht
- first_name: Andrej
full_name: Hlavacka, Andrej
last_name: Hlavacka
- first_name: Jozef
full_name: Šamaj, Jozef
last_name: Šamaj
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Theodorus
full_name: Gadella, Theodorus W
last_name: Gadella
citation:
ama: Dhonukshe P, Baluška F, Schlicht M, et al. Endocytosis of cell surface material
mediates cell plate formation during plant cytokinesis. Developmental Cell.
2006;10(1):137-150. doi:10.1016/j.devcel.2005.11.015
apa: Dhonukshe, P., Baluška, F., Schlicht, M., Hlavacka, A., Šamaj, J., Friml, J.,
& Gadella, T. (2006). Endocytosis of cell surface material mediates cell plate
formation during plant cytokinesis. Developmental Cell. Cell Press. https://doi.org/10.1016/j.devcel.2005.11.015
chicago: Dhonukshe, Pankaj, František Baluška, Markus Schlicht, Andrej Hlavacka,
Jozef Šamaj, Jiří Friml, and Theodorus Gadella. “Endocytosis of Cell Surface Material
Mediates Cell Plate Formation during Plant Cytokinesis.” Developmental Cell.
Cell Press, 2006. https://doi.org/10.1016/j.devcel.2005.11.015.
ieee: P. Dhonukshe et al., “Endocytosis of cell surface material mediates
cell plate formation during plant cytokinesis,” Developmental Cell, vol.
10, no. 1. Cell Press, pp. 137–150, 2006.
ista: Dhonukshe P, Baluška F, Schlicht M, Hlavacka A, Šamaj J, Friml J, Gadella
T. 2006. Endocytosis of cell surface material mediates cell plate formation during
plant cytokinesis. Developmental Cell. 10(1), 137–150.
mla: Dhonukshe, Pankaj, et al. “Endocytosis of Cell Surface Material Mediates Cell
Plate Formation during Plant Cytokinesis.” Developmental Cell, vol. 10,
no. 1, Cell Press, 2006, pp. 137–50, doi:10.1016/j.devcel.2005.11.015.
short: P. Dhonukshe, F. Baluška, M. Schlicht, A. Hlavacka, J. Šamaj, J. Friml, T.
Gadella, Developmental Cell 10 (2006) 137–150.
date_created: 2018-12-11T12:00:49Z
date_published: 2006-01-01T00:00:00Z
date_updated: 2021-01-12T07:40:24Z
day: '01'
doi: 10.1016/j.devcel.2005.11.015
extern: 1
intvolume: ' 10'
issue: '1'
month: '01'
page: 137 - 150
publication: Developmental Cell
publication_status: published
publisher: Cell Press
publist_id: '3696'
quality_controlled: 0
status: public
title: Endocytosis of cell surface material mediates cell plate formation during plant
cytokinesis
type: journal_article
volume: 10
year: '2006'
...
---
_id: '3007'
abstract:
- lang: eng
text: Root gravitropism describes the orientation of root growth along the gravity
vector and is mediated by differential cell elongation in the root meristem. This
response requires the coordinated, asymmetric distribution of the phytohormone
auxin within the root meristem, and depends on the concerted activities of PIN
proteins and AUX1 - members of the auxin transport pathway. Here, we show that
intracellular trafficking and proteasome activity combine to control PIN2 degradation
during root gravitropism. Following gravi-stimulation, proteasome-dependent variations
in PIN2 localization and degradation at the upper and lower sides of the root
result in asymmetric distribution of PIN2. Ubiquitination of PIN2 occurs in a
proteasome-dependent manner, indicating that the proteasome is involved in the
control of PIN2 turnover. Stabilization of PIN2 affects its abundance and distribution,
and leads to defects in auxin distribution and gravitropic responses. We describe
the effects of auxin on PIN2 localization and protein levels, indicating that
redistribution of auxin during the gravitropic response may be involved in the
regulation of PIN2 protein.
author:
- first_name: Lindy
full_name: Abas, Lindy
last_name: Abas
- first_name: René
full_name: Benjamins, René
last_name: Benjamins
- first_name: Nenad
full_name: Malenica, Nenad
last_name: Malenica
- first_name: Tomasz
full_name: Paciorek, Tomasz
last_name: Paciorek
- first_name: Justyna
full_name: Wiśniewska, Justyna
last_name: Wiśniewska
- first_name: Jeanette
full_name: Moulinier-Anzola, Jeanette C
last_name: Moulinier Anzola
- first_name: Tobias
full_name: Sieberer, Tobias
last_name: Sieberer
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Christian
full_name: Luschnig, Christian
last_name: Luschnig
citation:
ama: Abas L, Benjamins R, Malenica N, et al. Intracellular trafficking and proteolysis
of the Arabidopsis auxin-efflux facilitator PIN2 are involved in root gravitropism.
Nature Cell Biology. 2006;8(3):249-256. doi:10.1038/ncb1369
apa: Abas, L., Benjamins, R., Malenica, N., Paciorek, T., Wiśniewska, J., Moulinier
Anzola, J., … Luschnig, C. (2006). Intracellular trafficking and proteolysis of
the Arabidopsis auxin-efflux facilitator PIN2 are involved in root gravitropism.
Nature Cell Biology. Nature Publishing Group. https://doi.org/10.1038/ncb1369
chicago: Abas, Lindy, René Benjamins, Nenad Malenica, Tomasz Paciorek, Justyna Wiśniewska,
Jeanette Moulinier Anzola, Tobias Sieberer, Jiří Friml, and Christian Luschnig.
“Intracellular Trafficking and Proteolysis of the Arabidopsis Auxin-Efflux Facilitator
PIN2 Are Involved in Root Gravitropism.” Nature Cell Biology. Nature Publishing
Group, 2006. https://doi.org/10.1038/ncb1369.
ieee: L. Abas et al., “Intracellular trafficking and proteolysis of the Arabidopsis
auxin-efflux facilitator PIN2 are involved in root gravitropism,” Nature Cell
Biology, vol. 8, no. 3. Nature Publishing Group, pp. 249–256, 2006.
ista: Abas L, Benjamins R, Malenica N, Paciorek T, Wiśniewska J, Moulinier Anzola
J, Sieberer T, Friml J, Luschnig C. 2006. Intracellular trafficking and proteolysis
of the Arabidopsis auxin-efflux facilitator PIN2 are involved in root gravitropism.
Nature Cell Biology. 8(3), 249–256.
mla: Abas, Lindy, et al. “Intracellular Trafficking and Proteolysis of the Arabidopsis
Auxin-Efflux Facilitator PIN2 Are Involved in Root Gravitropism.” Nature Cell
Biology, vol. 8, no. 3, Nature Publishing Group, 2006, pp. 249–56, doi:10.1038/ncb1369.
short: L. Abas, R. Benjamins, N. Malenica, T. Paciorek, J. Wiśniewska, J. Moulinier
Anzola, T. Sieberer, J. Friml, C. Luschnig, Nature Cell Biology 8 (2006) 249–256.
date_created: 2018-12-11T12:00:50Z
date_published: 2006-03-01T00:00:00Z
date_updated: 2021-01-12T07:40:25Z
day: '01'
doi: 10.1038/ncb1369
extern: 1
intvolume: ' 8'
issue: '3'
month: '03'
page: 249 - 256
publication: Nature Cell Biology
publication_status: published
publisher: Nature Publishing Group
publist_id: '3694'
quality_controlled: 0
status: public
title: Intracellular trafficking and proteolysis of the Arabidopsis auxin-efflux facilitator
PIN2 are involved in root gravitropism
type: journal_article
volume: 8
year: '2006'
...
---
_id: '3008'
abstract:
- lang: eng
text: Plants and some animals have a profound capacity to regenerate organs from
adult tissues. Molecular mechanisms for regeneration have, however, been largely
unexplored. Here we investigate a local regeneration response in Arabidopsis roots.
Laser-induced wounding disrupts the flow of auxin-a cell-fate-instructive plant
hormone-in root tips, and we demonstrate that resulting cell-fate changes require
the PLETHORA, SHORTROOT, and SCARECROW transcription factors. These transcription
factors regulate the expression and polar position of PIN auxin efflux-facilitating
membrane proteins to reconstitute auxin transport in renewed root tips. Thus,
a regeneration mechanism using embryonic root stem-cell patterning factors first
responds to and subsequently stabilizes a new hormone distribution.
author:
- first_name: Jian
full_name: Xu, Jian
last_name: Xu
- first_name: Hugo
full_name: Hofhuis, Hugo
last_name: Hofhuis
- first_name: Renze
full_name: Heidstra, Renze
last_name: Heidstra
- first_name: Michael
full_name: Sauer, Michael
last_name: Sauer
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Ben
full_name: Scheres, Ben
last_name: Scheres
citation:
ama: Xu J, Hofhuis H, Heidstra R, Sauer M, Friml J, Scheres B. A molecular framework
for plant regeneration. Science. 2006;311(5759):385-388. doi:10.1126/science.1121790
apa: Xu, J., Hofhuis, H., Heidstra, R., Sauer, M., Friml, J., & Scheres, B.
(2006). A molecular framework for plant regeneration. Science. American
Association for the Advancement of Science. https://doi.org/10.1126/science.1121790
chicago: Xu, Jian, Hugo Hofhuis, Renze Heidstra, Michael Sauer, Jiří Friml, and
Ben Scheres. “A Molecular Framework for Plant Regeneration.” Science. American
Association for the Advancement of Science, 2006. https://doi.org/10.1126/science.1121790.
ieee: J. Xu, H. Hofhuis, R. Heidstra, M. Sauer, J. Friml, and B. Scheres, “A molecular
framework for plant regeneration,” Science, vol. 311, no. 5759. American
Association for the Advancement of Science, pp. 385–388, 2006.
ista: Xu J, Hofhuis H, Heidstra R, Sauer M, Friml J, Scheres B. 2006. A molecular
framework for plant regeneration. Science. 311(5759), 385–388.
mla: Xu, Jian, et al. “A Molecular Framework for Plant Regeneration.” Science,
vol. 311, no. 5759, American Association for the Advancement of Science, 2006,
pp. 385–88, doi:10.1126/science.1121790.
short: J. Xu, H. Hofhuis, R. Heidstra, M. Sauer, J. Friml, B. Scheres, Science 311
(2006) 385–388.
date_created: 2018-12-11T12:00:50Z
date_published: 2006-01-20T00:00:00Z
date_updated: 2021-01-12T07:40:25Z
day: '20'
doi: 10.1126/science.1121790
extern: 1
intvolume: ' 311'
issue: '5759'
month: '01'
page: 385 - 388
publication: Science
publication_status: published
publisher: American Association for the Advancement of Science
publist_id: '3695'
quality_controlled: 0
status: public
title: A molecular framework for plant regeneration
type: journal_article
volume: 311
year: '2006'
...
---
_id: '3009'
author:
- first_name: Tomasz
full_name: Paciorek, Tomasz
last_name: Paciorek
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Paciorek T, Friml J. Auxin signaling. Journal of Cell Science. 2006;119(7):1199-1202.
doi:10.1242/jcs.02910
apa: Paciorek, T., & Friml, J. (2006). Auxin signaling. Journal of Cell Science.
Company of Biologists. https://doi.org/10.1242/jcs.02910
chicago: Paciorek, Tomasz, and Jiří Friml. “Auxin Signaling.” Journal of Cell
Science. Company of Biologists, 2006. https://doi.org/10.1242/jcs.02910.
ieee: T. Paciorek and J. Friml, “Auxin signaling,” Journal of Cell Science,
vol. 119, no. 7. Company of Biologists, pp. 1199–1202, 2006.
ista: Paciorek T, Friml J. 2006. Auxin signaling. Journal of Cell Science. 119(7),
1199–1202.
mla: Paciorek, Tomasz, and Jiří Friml. “Auxin Signaling.” Journal of Cell Science,
vol. 119, no. 7, Company of Biologists, 2006, pp. 1199–202, doi:10.1242/jcs.02910.
short: T. Paciorek, J. Friml, Journal of Cell Science 119 (2006) 1199–1202.
date_created: 2018-12-11T12:00:50Z
date_published: 2006-01-01T00:00:00Z
date_updated: 2021-01-12T07:40:25Z
day: '01'
doi: 10.1242/jcs.02910
extern: '1'
external_id:
pmid:
- ' 16554435'
intvolume: ' 119'
issue: '7'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pubmed/16554435
month: '01'
oa: 1
oa_version: Published Version
page: 1199 - 1202
pmid: 1
publication: Journal of Cell Science
publication_status: published
publisher: Company of Biologists
publist_id: '3693'
quality_controlled: '1'
status: public
title: Auxin signaling
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 119
year: '2006'
...
---
_id: '3010'
abstract:
- lang: eng
text: The formation of the leaf vascular pattern has fascinated biologists for centuries.
In the early leaf primordium, complex networks of procambial cells emerge from
homogeneous subepidermal tissue. The molecular nature of the underlying positional
information is unknown, but various lines of evidence implicate gradually restricted
transport routes of the plant hormone auxin in defining sites of procambium formation.
Here we show that a crucial member of the AtPIN family of auxin-efflux-associated
proteins, AtPIN1, is expressed prior to pre-procambial and procambial cell fate
markers in domains that become restricted toward sites of procambium formation.
Subcellular AtPIN1 polarity indicates that auxin is directed to distinct "convergence
points" in the epidermis, from where it defines the positions of major veins.
Integrated polarities in all emerging veins indicate auxin drainage toward pre-existing
veins, but veins display divergent polarities as they become connected at both
ends. Auxin application and transport inhibition reveal that convergence point
positioning and AtPIN1 expression domain dynamics are self-organizing, auxin-transport-dependent
processes. We derive a model for self-regulated, reiterative patterning of all
vein orders and postulate at its onset a common epidermal auxin-focusing mechanism
for major-vein positioning and phyllotactic patterning.
author:
- first_name: Enrico
full_name: Scarpella, Enrico
last_name: Scarpella
- first_name: Danielle
full_name: Marcos, Danielle
last_name: Marcos
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Thomas
full_name: Berleth, Thomas
last_name: Berleth
citation:
ama: Scarpella E, Marcos D, Friml J, Berleth T. Control of leaf vascular patterning
by polar auxin transport. Genes and Development. 2006;20(8):1015-1027.
doi:10.1101/gad.1402406
apa: Scarpella, E., Marcos, D., Friml, J., & Berleth, T. (2006). Control of
leaf vascular patterning by polar auxin transport. Genes and Development.
Cold Spring Harbor Laboratory Press. https://doi.org/10.1101/gad.1402406
chicago: Scarpella, Enrico, Danielle Marcos, Jiří Friml, and Thomas Berleth. “Control
of Leaf Vascular Patterning by Polar Auxin Transport.” Genes and Development.
Cold Spring Harbor Laboratory Press, 2006. https://doi.org/10.1101/gad.1402406.
ieee: E. Scarpella, D. Marcos, J. Friml, and T. Berleth, “Control of leaf vascular
patterning by polar auxin transport,” Genes and Development, vol. 20, no.
8. Cold Spring Harbor Laboratory Press, pp. 1015–1027, 2006.
ista: Scarpella E, Marcos D, Friml J, Berleth T. 2006. Control of leaf vascular
patterning by polar auxin transport. Genes and Development. 20(8), 1015–1027.
mla: Scarpella, Enrico, et al. “Control of Leaf Vascular Patterning by Polar Auxin
Transport.” Genes and Development, vol. 20, no. 8, Cold Spring Harbor Laboratory
Press, 2006, pp. 1015–27, doi:10.1101/gad.1402406.
short: E. Scarpella, D. Marcos, J. Friml, T. Berleth, Genes and Development 20 (2006)
1015–1027.
date_created: 2018-12-11T12:00:51Z
date_published: 2006-04-15T00:00:00Z
date_updated: 2021-01-12T07:40:26Z
day: '15'
doi: 10.1101/gad.1402406
extern: 1
intvolume: ' 20'
issue: '8'
month: '04'
page: 1015 - 1027
publication: Genes and Development
publication_status: published
publisher: Cold Spring Harbor Laboratory Press
publist_id: '3692'
quality_controlled: 0
status: public
title: Control of leaf vascular patterning by polar auxin transport
type: journal_article
volume: 20
year: '2006'
...
---
_id: '3011'
abstract:
- lang: eng
text: Polar flow of the phytohormone auxin requires plasma membrane‐associated PIN
proteins and underlies multiple developmental processes in plants. Here we address
the importance of the polarity of subcellular PIN localization for the directionality
of auxin transport in Arabidopsis thaliana. Expression of different PINs in the
root epidermis revealed the importance of PIN polar positions for directional
auxin flow and root gravitropic growth. Interfering with sequence-embedded polarity
signals directly demonstrates that PIN polarity is a primary factor in determining
the direction of auxin flow in meristematic tissues. This finding provides a crucial
piece in the puzzle of how auxin flow can be redirected via rapid changes in PIN
polarity.
author:
- first_name: Justyna
full_name: Wiśniewska, Justyna
last_name: Wiśniewska
- first_name: Jian
full_name: Xu, Jian
last_name: Xu
- first_name: Daniela
full_name: Seifertová, Daniela
last_name: Seifertová
- first_name: Philip
full_name: Brewer, Philip B
last_name: Brewer
- first_name: Kamil
full_name: Růžička, Kamil
last_name: Růžička
- first_name: Ikram
full_name: Blilou, Ikram
last_name: Blilou
- first_name: David
full_name: Rouquié, David
last_name: Rouquié
- first_name: Eva
full_name: Eva Benková
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Ben
full_name: Scheres, Ben
last_name: Scheres
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Wiśniewska J, Xu J, Seifertová D, et al. Polar PIN localization directs auxin
flow in plants. Science. 2006;312(5775). doi:10.1126/science.1121356
apa: Wiśniewska, J., Xu, J., Seifertová, D., Brewer, P., Růžička, K., Blilou, I.,
… Friml, J. (2006). Polar PIN localization directs auxin flow in plants. Science.
American Association for the Advancement of Science. https://doi.org/10.1126/science.1121356
chicago: Wiśniewska, Justyna, Jian Xu, Daniela Seifertová, Philip Brewer, Kamil
Růžička, Ikram Blilou, David Rouquié, Eva Benková, Ben Scheres, and Jiří Friml.
“Polar PIN Localization Directs Auxin Flow in Plants.” Science. American
Association for the Advancement of Science, 2006. https://doi.org/10.1126/science.1121356.
ieee: J. Wiśniewska et al., “Polar PIN localization directs auxin flow in
plants,” Science, vol. 312, no. 5775. American Association for the Advancement
of Science, 2006.
ista: Wiśniewska J, Xu J, Seifertová D, Brewer P, Růžička K, Blilou I, Rouquié D,
Benková E, Scheres B, Friml J. 2006. Polar PIN localization directs auxin flow
in plants. Science. 312(5775).
mla: Wiśniewska, Justyna, et al. “Polar PIN Localization Directs Auxin Flow in Plants.”
Science, vol. 312, no. 5775, American Association for the Advancement of
Science, 2006, doi:10.1126/science.1121356.
short: J. Wiśniewska, J. Xu, D. Seifertová, P. Brewer, K. Růžička, I. Blilou, D.
Rouquié, E. Benková, B. Scheres, J. Friml, Science 312 (2006).
date_created: 2018-12-11T12:00:51Z
date_published: 2006-05-12T00:00:00Z
date_updated: 2021-01-12T07:40:27Z
day: '12'
doi: 10.1126/science.1121356
extern: 1
intvolume: ' 312'
issue: '5775'
month: '05'
publication: Science
publication_status: published
publisher: American Association for the Advancement of Science
publist_id: '3691'
quality_controlled: 0
status: public
title: Polar PIN localization directs auxin flow in plants
type: journal_article
volume: 312
year: '2006'
...
---
_id: '3012'
abstract:
- lang: eng
text: Intercellular flow of the phytohormone auxin underpins multiple developmental
processes in plants. Plant-specific pin-formed (PIN) proteins and several phosphoglycoprotein
(PGP) transporters are crucial factors in auxin transport-related development,
yet the molecular function of PINs remains unknown. Here, we show that PINs mediate
auxin efflux from mammalian and yeast cells without needing additional plant-specific
factors. Conditional gain-of-function alleles and quantitative measurements of
auxin accumulation in Arabidopsis and tobacco cultured cells revealed that the
action of PINs in auxin efflux is distinct from PGP, rate-limiting, specific to
auxins, and sensitive to auxin transport inhibitors. This suggests a direct involvement
of PINs in catalyzing cellular auxin efflux.
author:
- first_name: Jan
full_name: Petrášek, Jan
last_name: Petrášek
- first_name: Jozef
full_name: Mravec, Jozef
last_name: Mravec
- first_name: Rodolphe
full_name: Bouchard, Rodolphe
last_name: Bouchard
- first_name: Joshua
full_name: Blakeslee, Joshua
last_name: Blakeslee
- first_name: Melinda F
full_name: Melinda Abas
id: 3CFB3B1C-F248-11E8-B48F-1D18A9856A87
last_name: Abas
- first_name: Daniela
full_name: Seifertová, Daniela
last_name: Seifertová
- first_name: Justyna
full_name: Wiśniewska, Justyna
last_name: Wiśniewska
- first_name: Zerihun
full_name: Tadele, Zerihun
last_name: Tadele
- first_name: Martin
full_name: Kubeš, Martin
last_name: Kubeš
- first_name: Milada
full_name: Čovanová, Milada
last_name: Čovanová
- first_name: Pankaj
full_name: Dhonukshe, Pankaj
last_name: Dhonukshe
- first_name: Petr
full_name: Skůpa, Petr
last_name: Skůpa
- first_name: Eva
full_name: Eva Benková
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Lucie
full_name: Perry, Lucie
last_name: Perry
- first_name: Pavel
full_name: Křeček, Pavel
last_name: Křeček
- first_name: Ok
full_name: Lee, Ok Ran
last_name: Lee
- first_name: Gerald
full_name: Fink, Gerald R
last_name: Fink
- first_name: Markus
full_name: Geisler, Markus
last_name: Geisler
- first_name: Angus
full_name: Murphy, Angus S
last_name: Murphy
- first_name: Christian
full_name: Luschnig, Christian
last_name: Luschnig
- first_name: Eva
full_name: Zažímalová, Eva
last_name: Zažímalová
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Petrášek J, Mravec J, Bouchard R, et al. PIN proteins perform a rate-limiting
function in cellular auxin efflux. Science. 2006;312(5775):914-918. doi:10.1126/science.1123542
apa: Petrášek, J., Mravec, J., Bouchard, R., Blakeslee, J., Abas, M. F., Seifertová,
D., … Friml, J. (2006). PIN proteins perform a rate-limiting function in cellular
auxin efflux. Science. American Association for the Advancement of Science.
https://doi.org/10.1126/science.1123542
chicago: Petrášek, Jan, Jozef Mravec, Rodolphe Bouchard, Joshua Blakeslee, Melinda
F Abas, Daniela Seifertová, Justyna Wiśniewska, et al. “PIN Proteins Perform a
Rate-Limiting Function in Cellular Auxin Efflux.” Science. American Association
for the Advancement of Science, 2006. https://doi.org/10.1126/science.1123542.
ieee: J. Petrášek et al., “PIN proteins perform a rate-limiting function
in cellular auxin efflux,” Science, vol. 312, no. 5775. American Association
for the Advancement of Science, pp. 914–918, 2006.
ista: Petrášek J, Mravec J, Bouchard R, Blakeslee J, Abas MF, Seifertová D, Wiśniewska
J, Tadele Z, Kubeš M, Čovanová M, Dhonukshe P, Skůpa P, Benková E, Perry L, Křeček
P, Lee O, Fink G, Geisler M, Murphy A, Luschnig C, Zažímalová E, Friml J. 2006.
PIN proteins perform a rate-limiting function in cellular auxin efflux. Science.
312(5775), 914–918.
mla: Petrášek, Jan, et al. “PIN Proteins Perform a Rate-Limiting Function in Cellular
Auxin Efflux.” Science, vol. 312, no. 5775, American Association for the
Advancement of Science, 2006, pp. 914–18, doi:10.1126/science.1123542.
short: J. Petrášek, J. Mravec, R. Bouchard, J. Blakeslee, M.F. Abas, D. Seifertová,
J. Wiśniewska, Z. Tadele, M. Kubeš, M. Čovanová, P. Dhonukshe, P. Skůpa, E. Benková,
L. Perry, P. Křeček, O. Lee, G. Fink, M. Geisler, A. Murphy, C. Luschnig, E. Zažímalová,
J. Friml, Science 312 (2006) 914–918.
date_created: 2018-12-11T12:00:51Z
date_published: 2006-05-12T00:00:00Z
date_updated: 2021-01-12T07:40:27Z
day: '12'
doi: 10.1126/science.1123542
extern: 1
intvolume: ' 312'
issue: '5775'
month: '05'
page: 914 - 918
publication: Science
publication_status: published
publisher: American Association for the Advancement of Science
publist_id: '3690'
quality_controlled: 0
status: public
title: PIN proteins perform a rate-limiting function in cellular auxin efflux
type: journal_article
volume: 312
year: '2006'
...
---
_id: '3013'
abstract:
- lang: eng
text: 'There is a growing demand for methods that allow rapid and reliable in situ
localization of proteins in plant cells. The immunocytochemistry protocol presented
here can be used routinely to observe protein localization patterns in tissue
sections of various plant species. This protocol is especially suitable for plant
species with more-complex tissue architecture (such as maize, Zea mays), which
makes it difficult to use an easier whole-mount procedure for protein localization.
To facilitate the antibody-antigen reaction, it is necessary to include a wax-embedding
and tissue-sectioning step. The protocol consists of the following procedures:
chemical fixation of tissue, dehydration, wax embedding, sectioning, dewaxing,
rehydration, blocking and antibody incubation. The detailed protocol, recommended
controls and troubleshooting are presented here, along with examples of applications.'
author:
- first_name: Tomasz
full_name: Paciorek, Tomasz
last_name: Paciorek
- first_name: Michael
full_name: Sauer, Michael
last_name: Sauer
- first_name: Jozef
full_name: Balla, Jozef
last_name: Balla
- first_name: Justyna
full_name: Wiśniewska, Justyna
last_name: Wiśniewska
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Paciorek T, Sauer M, Balla J, Wiśniewska J, Friml J. Immunocytochemical technique
for protein localization in sections of plant tissues. Nature Protocols.
2006;1(1):104-107. doi:10.1038/nprot.2006.16
apa: Paciorek, T., Sauer, M., Balla, J., Wiśniewska, J., & Friml, J. (2006).
Immunocytochemical technique for protein localization in sections of plant tissues.
Nature Protocols. Nature Publishing Group. https://doi.org/10.1038/nprot.2006.16
chicago: Paciorek, Tomasz, Michael Sauer, Jozef Balla, Justyna Wiśniewska, and Jiří
Friml. “Immunocytochemical Technique for Protein Localization in Sections of Plant
Tissues.” Nature Protocols. Nature Publishing Group, 2006. https://doi.org/10.1038/nprot.2006.16.
ieee: T. Paciorek, M. Sauer, J. Balla, J. Wiśniewska, and J. Friml, “Immunocytochemical
technique for protein localization in sections of plant tissues,” Nature Protocols,
vol. 1, no. 1. Nature Publishing Group, pp. 104–107, 2006.
ista: Paciorek T, Sauer M, Balla J, Wiśniewska J, Friml J. 2006. Immunocytochemical
technique for protein localization in sections of plant tissues. Nature Protocols.
1(1), 104–107.
mla: Paciorek, Tomasz, et al. “Immunocytochemical Technique for Protein Localization
in Sections of Plant Tissues.” Nature Protocols, vol. 1, no. 1, Nature
Publishing Group, 2006, pp. 104–07, doi:10.1038/nprot.2006.16.
short: T. Paciorek, M. Sauer, J. Balla, J. Wiśniewska, J. Friml, Nature Protocols
1 (2006) 104–107.
date_created: 2018-12-11T12:00:52Z
date_published: 2006-06-01T00:00:00Z
date_updated: 2021-01-12T07:40:27Z
day: '01'
doi: 10.1038/nprot.2006.16
extern: 1
intvolume: ' 1'
issue: '1'
month: '06'
page: 104 - 107
publication: Nature Protocols
publication_status: published
publisher: Nature Publishing Group
publist_id: '3689'
quality_controlled: 0
status: public
title: Immunocytochemical technique for protein localization in sections of plant
tissues
type: journal_article
volume: 1
year: '2006'
...
---
_id: '3014'
abstract:
- lang: eng
text: Plant biology is currently confronted with an overflow of expression profile
data provided by high-throughput microarray transcription analyses. However, the
tissue and cellular resolution of these techniques is limited. Thus, it is still
necessary to examine the expression pattern of selected candidate genes at a cellular
level. Here we present an in situ mRNA hybridization method that is routinely
used in the analysis of gene expression patterns. The protocol is optimized for
mRNA localizations in sectioned tissue of Arabidopsis seedlings including embryos,
roots, hypocotyls, young primary leaves and flowers. The detailed protocol, recommended
controls and troubleshooting are presented along with examples of application.
The total time for the process is 10 days.
author:
- first_name: Philip
full_name: Brewer, Philip B
last_name: Brewer
- first_name: Marcus
full_name: Heisler, Marcus G
last_name: Heisler
- first_name: Jan
full_name: Hejátko, Jan
last_name: Hejátko
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Eva
full_name: Eva Benková
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
citation:
ama: Brewer P, Heisler M, Hejátko J, Friml J, Benková E. In situ hybridization for
mRNA detection in Arabidopsis tissue sections. Nature Protocols. 2006;1(3):1462-1467.
doi:10.1038/nprot.2006.226
apa: Brewer, P., Heisler, M., Hejátko, J., Friml, J., & Benková, E. (2006).
In situ hybridization for mRNA detection in Arabidopsis tissue sections. Nature
Protocols. Nature Publishing Group. https://doi.org/10.1038/nprot.2006.226
chicago: Brewer, Philip, Marcus Heisler, Jan Hejátko, Jiří Friml, and Eva Benková.
“In Situ Hybridization for MRNA Detection in Arabidopsis Tissue Sections.” Nature
Protocols. Nature Publishing Group, 2006. https://doi.org/10.1038/nprot.2006.226.
ieee: P. Brewer, M. Heisler, J. Hejátko, J. Friml, and E. Benková, “In situ hybridization
for mRNA detection in Arabidopsis tissue sections,” Nature Protocols, vol.
1, no. 3. Nature Publishing Group, pp. 1462–1467, 2006.
ista: Brewer P, Heisler M, Hejátko J, Friml J, Benková E. 2006. In situ hybridization
for mRNA detection in Arabidopsis tissue sections. Nature Protocols. 1(3), 1462–1467.
mla: Brewer, Philip, et al. “In Situ Hybridization for MRNA Detection in Arabidopsis
Tissue Sections.” Nature Protocols, vol. 1, no. 3, Nature Publishing Group,
2006, pp. 1462–67, doi:10.1038/nprot.2006.226.
short: P. Brewer, M. Heisler, J. Hejátko, J. Friml, E. Benková, Nature Protocols
1 (2006) 1462–1467.
date_created: 2018-12-11T12:00:52Z
date_published: 2006-08-01T00:00:00Z
date_updated: 2021-01-12T07:40:28Z
day: '01'
doi: 10.1038/nprot.2006.226
extern: 1
intvolume: ' 1'
issue: '3'
month: '08'
page: 1462 - 1467
publication: Nature Protocols
publication_status: published
publisher: Nature Publishing Group
publist_id: '3687'
quality_controlled: 0
status: public
title: In situ hybridization for mRNA detection in Arabidopsis tissue sections
type: journal_article
volume: 1
year: '2006'
...
---
_id: '3015'
abstract:
- lang: eng
text: 'As the field of plant molecular biology is swiftly advancing, a need has
been created for methods that allow rapid and reliable in situ localization of
proteins in plant cells. Here we describe a whole-mount ''immunolocalization''
technique for various plant tissues, including roots, hypocotyls, cotyledons,
young primary leaves and embryos of Arabidopsis thaliana and other species. The
detailed protocol, recommended controls and troubleshooting are presented, along
with examples of applications. The protocol consists of five main procedures:
tissue fixation, tissue permeation, blocking, primary and secondary antibody incubation.
Notably, the first procedure (tissue fixation) includes several steps (4-12) that
are absolutely necessary for protein localization in hypocotyls, cotyledons and
young primary leaves but should be omitted for other tissues. The protocol is
usually done in 3 days, but could also be completed in 2 days.'
author:
- first_name: Michael
full_name: Sauer, Michael
last_name: Sauer
- first_name: Tomasz
full_name: Paciorek, Tomasz
last_name: Paciorek
- first_name: Eva
full_name: Eva Benková
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Sauer M, Paciorek T, Benková E, Friml J. Immunocytochemical techniques for
whole mount in situ protein localization in plants. Nature Protocols. 2006;1(1):98-103.
doi:10.1038/nprot.2006.15
apa: Sauer, M., Paciorek, T., Benková, E., & Friml, J. (2006). Immunocytochemical
techniques for whole mount in situ protein localization in plants. Nature Protocols.
Nature Publishing Group. https://doi.org/10.1038/nprot.2006.15
chicago: Sauer, Michael, Tomasz Paciorek, Eva Benková, and Jiří Friml. “Immunocytochemical
Techniques for Whole Mount in Situ Protein Localization in Plants.” Nature
Protocols. Nature Publishing Group, 2006. https://doi.org/10.1038/nprot.2006.15.
ieee: M. Sauer, T. Paciorek, E. Benková, and J. Friml, “Immunocytochemical techniques
for whole mount in situ protein localization in plants,” Nature Protocols,
vol. 1, no. 1. Nature Publishing Group, pp. 98–103, 2006.
ista: Sauer M, Paciorek T, Benková E, Friml J. 2006. Immunocytochemical techniques
for whole mount in situ protein localization in plants. Nature Protocols. 1(1),
98–103.
mla: Sauer, Michael, et al. “Immunocytochemical Techniques for Whole Mount in Situ
Protein Localization in Plants.” Nature Protocols, vol. 1, no. 1, Nature
Publishing Group, 2006, pp. 98–103, doi:10.1038/nprot.2006.15.
short: M. Sauer, T. Paciorek, E. Benková, J. Friml, Nature Protocols 1 (2006) 98–103.
date_created: 2018-12-11T12:00:52Z
date_published: 2006-06-01T00:00:00Z
date_updated: 2021-01-12T07:40:28Z
day: '01'
doi: 10.1038/nprot.2006.15
extern: 1
intvolume: ' 1'
issue: '1'
month: '06'
page: 98 - 103
publication: Nature Protocols
publication_status: published
publisher: Nature Publishing Group
publist_id: '3688'
quality_controlled: 0
status: public
title: Immunocytochemical techniques for whole mount in situ protein localization
in plants
type: journal_article
volume: 1
year: '2006'
...
---
_id: '3016'
abstract:
- lang: eng
text: Plant development is characterized by a profound ability to regenerate and
form tissues with new axes of polarity. An unsolved question concerns how the
position within a tissue and cues from neighboring cells are integrated to specify
the polarity of individual cells. The canalization hypothesis proposes a feedback
effect of the phytohormone auxin on the directionality of intercellular auxin
flow as a means to polarize tissues. Here we identify a cellular and molecular
mechanism for canalization. Local auxin application, wounding, or auxin accumulation
during de novo organ formation lead to rearrangements in the subcellular polar
localization of PIN auxin transport components. This auxin effect on PIN polarity
is cell-specific, does not depend on PIN transcription, and involves the Aux/IAA-ARF
(indole-3-acetic acid-auxin response factor) signaling pathway. Our data suggest
that auxin acts as polarizing cue, which links individual cell polarity with tissue
and organ polarity through control of PIN polar targeting. This feedback regulation
provides a conceptual framework for polarization during multiple regenerative
and patterning processes in plants.
article_processing_charge: No
author:
- first_name: Michael
full_name: Sauer, Michael
last_name: Sauer
- first_name: Jozef
full_name: Balla, Jozef
last_name: Balla
- first_name: Christian
full_name: Luschnig, Christian
last_name: Luschnig
- first_name: Justyna
full_name: Wiśniewska, Justyna
last_name: Wiśniewska
- first_name: Vilém
full_name: Reinöhl, Vilém
last_name: Reinöhl
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
citation:
ama: Sauer M, Balla J, Luschnig C, et al. Canalization of auxin flow by Aux/IAA-ARF-dependent
feedback regulation of PIN polarity. Genes and Development. 2006;20(20):2902-2911.
doi:10.1101/gad.390806
apa: Sauer, M., Balla, J., Luschnig, C., Wiśniewska, J., Reinöhl, V., Friml, J.,
& Benková, E. (2006). Canalization of auxin flow by Aux/IAA-ARF-dependent
feedback regulation of PIN polarity. Genes and Development. Cold Spring
Harbor Laboratory Press. https://doi.org/10.1101/gad.390806
chicago: Sauer, Michael, Jozef Balla, Christian Luschnig, Justyna Wiśniewska, Vilém
Reinöhl, Jiří Friml, and Eva Benková. “Canalization of Auxin Flow by Aux/IAA-ARF-Dependent
Feedback Regulation of PIN Polarity.” Genes and Development. Cold Spring
Harbor Laboratory Press, 2006. https://doi.org/10.1101/gad.390806.
ieee: M. Sauer et al., “Canalization of auxin flow by Aux/IAA-ARF-dependent
feedback regulation of PIN polarity,” Genes and Development, vol. 20, no.
20. Cold Spring Harbor Laboratory Press, pp. 2902–2911, 2006.
ista: Sauer M, Balla J, Luschnig C, Wiśniewska J, Reinöhl V, Friml J, Benková E.
2006. Canalization of auxin flow by Aux/IAA-ARF-dependent feedback regulation
of PIN polarity. Genes and Development. 20(20), 2902–2911.
mla: Sauer, Michael, et al. “Canalization of Auxin Flow by Aux/IAA-ARF-Dependent
Feedback Regulation of PIN Polarity.” Genes and Development, vol. 20, no.
20, Cold Spring Harbor Laboratory Press, 2006, pp. 2902–11, doi:10.1101/gad.390806.
short: M. Sauer, J. Balla, C. Luschnig, J. Wiśniewska, V. Reinöhl, J. Friml, E.
Benková, Genes and Development 20 (2006) 2902–2911.
date_created: 2018-12-11T12:00:53Z
date_published: 2006-10-15T00:00:00Z
date_updated: 2021-11-16T07:53:09Z
day: '15'
doi: 10.1101/gad.390806
extern: '1'
intvolume: ' 20'
issue: '20'
language:
- iso: eng
month: '10'
oa_version: None
page: 2902 - 2911
publication: Genes and Development
publication_status: published
publisher: Cold Spring Harbor Laboratory Press
publist_id: '3686'
related_material:
link:
- relation: erratum
url: http://genesdev.cshlp.org/content/21/11/1431.short
status: public
title: Canalization of auxin flow by Aux/IAA-ARF-dependent feedback regulation of
PIN polarity
type: journal_article
user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9
volume: 20
year: '2006'
...
---
_id: '3017'
abstract:
- lang: eng
text: The plant hormone auxin plays crucial roles in regulating plant growth development,
including embryo and root patterning, organ formation, vascular tissue differentiation
and growth responses to environmental stimuli. Asymmetric auxin distribution patterns
have been observed within tissues, and these so-called auxin gradients change
dynamically during different developmental processes. Most auxin is synthesized
in the shoot and distributed directionally throughout the plant. This polar auxin
transport is mediated by auxin influx and efflux facilitators, whose subcellular
polar localizations guide the direction of auxin flow. The polar localization
of PIN auxin efflux carriers changes in response to developmental and external
cues in order to channel auxin flow in a regulated manner for organized growth.
Auxin itself modulates the expression and subcellular localization of PIN proteins,
contributing to a complex pattern of feedback regulation. Here we review the available
information mainly from studies of a model plant, Arabidopsis thaliana, on the
generation of auxin gradients, the regulation of polar auxin transport and further
downstream cellular events.
author:
- first_name: Hirokazu
full_name: Tanaka, Hirokazu
last_name: Tanaka
- first_name: Pankaj
full_name: Dhonukshe, Pankaj
last_name: Dhonukshe
- first_name: Philip
full_name: Brewer, Philip
last_name: Brewer
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: 'Tanaka H, Dhonukshe P, Brewer P, Friml J. Spatiotemporal asymmetric auxin
distribution: A means to coordinate plant development. Cellular and Molecular
Life Sciences. 2006;63(23):2738-2754. doi:10.1007/s00018-006-6116-5'
apa: 'Tanaka, H., Dhonukshe, P., Brewer, P., & Friml, J. (2006). Spatiotemporal
asymmetric auxin distribution: A means to coordinate plant development. Cellular
and Molecular Life Sciences. Birkhäuser. https://doi.org/10.1007/s00018-006-6116-5'
chicago: 'Tanaka, Hirokazu, Pankaj Dhonukshe, Philip Brewer, and Jiří Friml. “Spatiotemporal
Asymmetric Auxin Distribution: A Means to Coordinate Plant Development.” Cellular
and Molecular Life Sciences. Birkhäuser, 2006. https://doi.org/10.1007/s00018-006-6116-5.'
ieee: 'H. Tanaka, P. Dhonukshe, P. Brewer, and J. Friml, “Spatiotemporal asymmetric
auxin distribution: A means to coordinate plant development,” Cellular and
Molecular Life Sciences, vol. 63, no. 23. Birkhäuser, pp. 2738–2754, 2006.'
ista: 'Tanaka H, Dhonukshe P, Brewer P, Friml J. 2006. Spatiotemporal asymmetric
auxin distribution: A means to coordinate plant development. Cellular and Molecular
Life Sciences. 63(23), 2738–2754.'
mla: 'Tanaka, Hirokazu, et al. “Spatiotemporal Asymmetric Auxin Distribution: A
Means to Coordinate Plant Development.” Cellular and Molecular Life Sciences,
vol. 63, no. 23, Birkhäuser, 2006, pp. 2738–54, doi:10.1007/s00018-006-6116-5.'
short: H. Tanaka, P. Dhonukshe, P. Brewer, J. Friml, Cellular and Molecular Life
Sciences 63 (2006) 2738–2754.
date_created: 2018-12-11T12:00:53Z
date_published: 2006-12-01T00:00:00Z
date_updated: 2021-01-12T07:40:29Z
day: '01'
doi: 10.1007/s00018-006-6116-5
extern: '1'
intvolume: ' 63'
issue: '23'
language:
- iso: eng
month: '12'
oa_version: None
page: 2738 - 2754
publication: Cellular and Molecular Life Sciences
publication_status: published
publisher: Birkhäuser
publist_id: '3685'
quality_controlled: '1'
status: public
title: 'Spatiotemporal asymmetric auxin distribution: A means to coordinate plant
development'
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 63
year: '2006'
...
---
_id: '3018'
abstract:
- lang: eng
text: The directional flow of the plant hormone auxin mediates multiple developmental
processes, including patterning and tropisms. Apical and basal plasma membrane
localization of AUXIN-RESISTANT1 (AUX1) and PIN-FORMED1 (PIN1) auxin transport
components underpins the directionality of intercellular auxin flow in Arabidopsis
thaliana roots. Here, we examined the mechanism of polar trafficking of AUX1.
Real-time live cell analysis along with subcellular markers revealed that AUX1
resides at the apical plasma membrane of protophloem cells and at highly dynamic
subpopulations of Golgi apparatus and endosomes in all cell types. Plasma membrane
and intracellular pools of AUX1 are interconnected by actin-dependent constitutive
trafficking, which is not sensitive to the vesicle trafficking inhibitor brefeldin
A. AUX1 subcellular dynamics are not influenced by the auxin influx inhibitor
NOA but are blocked by the auxin efflux inhibitors TIBA and PBA. Furthermore,
auxin transport inhibitors and interference with the sterol composition of membranes
disrupt polar AUX1 distribution at the plasma membrane. Compared with PIN1 trafficking,
AUX1 dynamics display different sensitivities to trafficking inhibitors and are
independent of the endosomal trafficking regulator ARF GEF GNOM. Hence, AUX1 uses
a novel trafficking pathway in plants that is distinct from PIN trafficking, providing
an additional mechanism for the fine regulation of auxin transport.
author:
- first_name: Jürgen
full_name: Kleine-Vehn, Jürgen
last_name: Kleine Vehn
- first_name: Pankaj
full_name: Dhonukshe, Pankaj
last_name: Dhonukshe
- first_name: Ranjan
full_name: Swarup, Ranjan
last_name: Swarup
- first_name: Malcolm
full_name: Bennett, Malcolm
last_name: Bennett
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Kleine Vehn J, Dhonukshe P, Swarup R, Bennett M, Friml J. Subcellular trafficking
of the Arabidopsis auxin influx carrier AUX1 uses a novel pathway distinct from
PIN1. Plant Cell. 2006;18(11):3171-3181. doi:10.1105/tpc.106.042770
apa: Kleine Vehn, J., Dhonukshe, P., Swarup, R., Bennett, M., & Friml, J. (2006).
Subcellular trafficking of the Arabidopsis auxin influx carrier AUX1 uses a novel
pathway distinct from PIN1. Plant Cell. American Society of Plant Biologists.
https://doi.org/10.1105/tpc.106.042770
chicago: Kleine Vehn, Jürgen, Pankaj Dhonukshe, Ranjan Swarup, Malcolm Bennett,
and Jiří Friml. “Subcellular Trafficking of the Arabidopsis Auxin Influx Carrier
AUX1 Uses a Novel Pathway Distinct from PIN1.” Plant Cell. American Society
of Plant Biologists, 2006. https://doi.org/10.1105/tpc.106.042770.
ieee: J. Kleine Vehn, P. Dhonukshe, R. Swarup, M. Bennett, and J. Friml, “Subcellular
trafficking of the Arabidopsis auxin influx carrier AUX1 uses a novel pathway
distinct from PIN1,” Plant Cell, vol. 18, no. 11. American Society of Plant
Biologists, pp. 3171–3181, 2006.
ista: Kleine Vehn J, Dhonukshe P, Swarup R, Bennett M, Friml J. 2006. Subcellular
trafficking of the Arabidopsis auxin influx carrier AUX1 uses a novel pathway
distinct from PIN1. Plant Cell. 18(11), 3171–3181.
mla: Kleine Vehn, Jürgen, et al. “Subcellular Trafficking of the Arabidopsis Auxin
Influx Carrier AUX1 Uses a Novel Pathway Distinct from PIN1.” Plant Cell,
vol. 18, no. 11, American Society of Plant Biologists, 2006, pp. 3171–81, doi:10.1105/tpc.106.042770.
short: J. Kleine Vehn, P. Dhonukshe, R. Swarup, M. Bennett, J. Friml, Plant Cell
18 (2006) 3171–3181.
date_created: 2018-12-11T12:00:53Z
date_published: 2006-11-01T00:00:00Z
date_updated: 2021-01-12T07:40:29Z
day: '01'
doi: 10.1105/tpc.106.042770
extern: 1
intvolume: ' 18'
issue: '11'
month: '11'
page: 3171 - 3181
publication: Plant Cell
publication_status: published
publisher: American Society of Plant Biologists
publist_id: '3684'
quality_controlled: 0
status: public
title: Subcellular trafficking of the Arabidopsis auxin influx carrier AUX1 uses a
novel pathway distinct from PIN1
type: journal_article
volume: 18
year: '2006'
...
---
_id: '3020'
abstract:
- lang: eng
text: High throughput microarray transcription analyses provide us with the expression
profiles for large amounts of plant genes. However, their tissue and cellular
resolution is limited. Thus, for detailed functional analysis, it is still necessary
to examine the expression pattern of selected candidate genes at a cellular level.
Here, we present an in situ mRNA hybridization method that is routinely used for
the analysis of plant gene expression patterns. The protocol is optimized for
whole mount mRNA localizations in Arabidopsis seedling tissues including embryos,
roots, hypocotyls and young primary leaves. It can also be used for comparable
tissues in other species. Part of the protocol can also be automated and performed
by a liquid handling robot. Here we present a detailed protocol, recommended controls
and troubleshooting, along with examples of several applications. The total time
to carry out the entire procedure is ∼7 d, depending on the tissue used.
author:
- first_name: Jan
full_name: Hejátko, Jan
last_name: Hejátko
- first_name: Ikram
full_name: Blilou, Ikram
last_name: Blilou
- first_name: Philip
full_name: Brewer, Philip B
last_name: Brewer
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Ben
full_name: Scheres, Ben
last_name: Scheres
- first_name: Eva
full_name: Eva Benková
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
citation:
ama: Hejátko J, Blilou I, Brewer P, Friml J, Scheres B, Benková E. In situ hybridization
technique for mRNA detection in whole mount Arabidopsis samples. Nature Protocols.
2006;1(4):1939-1946. doi:10.1038/nprot.2006.333
apa: Hejátko, J., Blilou, I., Brewer, P., Friml, J., Scheres, B., & Benková,
E. (2006). In situ hybridization technique for mRNA detection in whole mount Arabidopsis
samples. Nature Protocols. Nature Publishing Group. https://doi.org/10.1038/nprot.2006.333
chicago: Hejátko, Jan, Ikram Blilou, Philip Brewer, Jiří Friml, Ben Scheres, and
Eva Benková. “In Situ Hybridization Technique for MRNA Detection in Whole Mount
Arabidopsis Samples.” Nature Protocols. Nature Publishing Group, 2006.
https://doi.org/10.1038/nprot.2006.333.
ieee: J. Hejátko, I. Blilou, P. Brewer, J. Friml, B. Scheres, and E. Benková, “In
situ hybridization technique for mRNA detection in whole mount Arabidopsis samples,”
Nature Protocols, vol. 1, no. 4. Nature Publishing Group, pp. 1939–1946,
2006.
ista: Hejátko J, Blilou I, Brewer P, Friml J, Scheres B, Benková E. 2006. In situ
hybridization technique for mRNA detection in whole mount Arabidopsis samples.
Nature Protocols. 1(4), 1939–1946.
mla: Hejátko, Jan, et al. “In Situ Hybridization Technique for MRNA Detection in
Whole Mount Arabidopsis Samples.” Nature Protocols, vol. 1, no. 4, Nature
Publishing Group, 2006, pp. 1939–46, doi:10.1038/nprot.2006.333.
short: J. Hejátko, I. Blilou, P. Brewer, J. Friml, B. Scheres, E. Benková, Nature
Protocols 1 (2006) 1939–1946.
date_created: 2018-12-11T12:00:54Z
date_published: 2006-11-01T00:00:00Z
date_updated: 2021-01-12T07:40:30Z
day: '01'
doi: 10.1038/nprot.2006.333
extern: 1
intvolume: ' 1'
issue: '4'
month: '11'
page: 1939 - 1946
publication: Nature Protocols
publication_status: published
publisher: Nature Publishing Group
publist_id: '3683'
quality_controlled: 0
status: public
title: In situ hybridization technique for mRNA detection in whole mount Arabidopsis
samples
type: journal_article
volume: 1
year: '2006'
...
---
_id: '3152'
abstract:
- lang: eng
text: The basic concepts of the molecular machinery that mediates cell migration
have been gleaned from cell culture systems. However, the three-dimensional environment
within an organism presents migrating cells with a much greater challenge. They
must move between and among other cells while interpreting multiple attractive
and repulsive cues to choose their proper path. They must coordinate their cell
adhesion with their surroundings and know when to start and stop moving. New insights
into the control of these remaining mysteries have emerged from genetic dissection
and live imaging of germ cell migration in Drosophila, zebrafish, and mouse embryos.
In this review, we first describe germ cell migration in cellular and mechanistic
detail in these different model systems. We then compare these systems to highlight
the emerging principles. Finally, we contrast the migration of germ cells with
that of immune and cancer cells to outline the conserved and different mechanisms.
author:
- first_name: Prabhat
full_name: Kunwar, Prabhat S
last_name: Kunwar
- first_name: Daria E
full_name: Daria Siekhaus
id: 3D224B9E-F248-11E8-B48F-1D18A9856A87
last_name: Siekhaus
orcid: 0000-0001-8323-8353
- first_name: Ruth
full_name: Lehmann, Ruth
last_name: Lehmann
citation:
ama: Kunwar P, Siekhaus DE, Lehmann R. In vivo migration A germ cell perspective.
Annual Review of Cell and Developmental Biology. 2006;22:237-265. doi:10.1146/annurev.cellbio.22.010305.103337
apa: Kunwar, P., Siekhaus, D. E., & Lehmann, R. (2006). In vivo migration A
germ cell perspective. Annual Review of Cell and Developmental Biology.
Annual Reviews. https://doi.org/10.1146/annurev.cellbio.22.010305.103337
chicago: Kunwar, Prabhat, Daria E Siekhaus, and Ruth Lehmann. “In Vivo Migration
A Germ Cell Perspective.” Annual Review of Cell and Developmental Biology.
Annual Reviews, 2006. https://doi.org/10.1146/annurev.cellbio.22.010305.103337.
ieee: P. Kunwar, D. E. Siekhaus, and R. Lehmann, “In vivo migration A germ cell
perspective,” Annual Review of Cell and Developmental Biology, vol. 22.
Annual Reviews, pp. 237–265, 2006.
ista: Kunwar P, Siekhaus DE, Lehmann R. 2006. In vivo migration A germ cell perspective.
Annual Review of Cell and Developmental Biology. 22, 237–265.
mla: Kunwar, Prabhat, et al. “In Vivo Migration A Germ Cell Perspective.” Annual
Review of Cell and Developmental Biology, vol. 22, Annual Reviews, 2006, pp.
237–65, doi:10.1146/annurev.cellbio.22.010305.103337.
short: P. Kunwar, D.E. Siekhaus, R. Lehmann, Annual Review of Cell and Developmental
Biology 22 (2006) 237–265.
date_created: 2018-12-11T12:01:42Z
date_published: 2006-06-14T00:00:00Z
date_updated: 2021-01-12T07:41:25Z
day: '14'
doi: 10.1146/annurev.cellbio.22.010305.103337
extern: 1
intvolume: ' 22'
month: '06'
page: 237 - 265
publication: Annual Review of Cell and Developmental Biology
publication_status: published
publisher: Annual Reviews
publist_id: '3543'
quality_controlled: 0
status: public
title: In vivo migration A germ cell perspective
type: journal_article
volume: 22
year: '2006'
...