---
_id: '2662'
abstract:
- lang: eng
text: G-protein-coupled inwardly rectifying K+ channels (Kir3 channels) coupled
to metabotropic GABAB receptors are essential for the control of neuronal excitation.
To determine the distribution of Kir3 channels and their spatial relationship
to GABAB receptors on hippocampal pyramidal cells, we used a high-resolution immunocytochemical
approach. Immunoreactivity for the Kir3.2 subunit was most abundant postsynaptically
and localized to the extrasynaptic plasma membrane of dendritic shafts and spines
of principal cells. Quantitative analysis of immunogold particles for Kir3.2 revealed
an enrichment of the protein around putative glutamatergic synapses on dendritic
spines, similar to that of GABA B1. Consistent with this observation, a high degree
of coclustering of Kir3.2 and GABAB1 was revealed around excitatory synapses by
the highly sensitive SDS-digested freeze-fracture replica immunolabeling. In contrast,
in dendritic shafts receptors and channels were found to be mainly segregated.
These results suggest that Kir3.2-containing K+ channels on dendritic spines preferentially
mediate the effect of GABA, whereas channels on dendritic shafts are likely to
be activated by other neurotransmitters as well. Thus, Kir3 channels, localized
to different subcellular compartments of hippocampal principal cells, appear to
be differentially involved in synaptic integration in pyramidal cell dendrites.
author:
- first_name: Ákos
full_name: Kulik, Ákos
last_name: Kulik
- first_name: Imre
full_name: Vida, Imre
last_name: Vida
- first_name: Yugo
full_name: Fukazawa, Yugo
last_name: Fukazawa
- first_name: Nicole
full_name: Guetg, Nicole
last_name: Guetg
- first_name: Yu
full_name: Kasugai, Yu
last_name: Kasugai
- first_name: Cheryl
full_name: Marker, Cheryl L
last_name: Marker
- first_name: Franck
full_name: Rigato, Franck
last_name: Rigato
- first_name: Bernhard
full_name: Bettler, Bernhard
last_name: Bettler
- first_name: Kevin
full_name: Wickman, Kevin D
last_name: Wickman
- first_name: Michael
full_name: Frotscher, Michael
last_name: Frotscher
- first_name: Ryuichi
full_name: Ryuichi Shigemoto
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
citation:
ama: Kulik Á, Vida I, Fukazawa Y, et al. Compartment-dependent colocalization of
Kir3.2-containing K+ channels and GABAB receptors in hippocampal pyramidal cells.
Journal of Neuroscience. 2006;26(16):4289-4297. doi:10.1523/JNEUROSCI.4178-05.2006
apa: Kulik, Á., Vida, I., Fukazawa, Y., Guetg, N., Kasugai, Y., Marker, C., … Shigemoto,
R. (2006). Compartment-dependent colocalization of Kir3.2-containing K+ channels
and GABAB receptors in hippocampal pyramidal cells. Journal of Neuroscience.
Society for Neuroscience. https://doi.org/10.1523/JNEUROSCI.4178-05.2006
chicago: Kulik, Ákos, Imre Vida, Yugo Fukazawa, Nicole Guetg, Yu Kasugai, Cheryl
Marker, Franck Rigato, et al. “Compartment-Dependent Colocalization of Kir3.2-Containing
K+ Channels and GABAB Receptors in Hippocampal Pyramidal Cells.” Journal of
Neuroscience. Society for Neuroscience, 2006. https://doi.org/10.1523/JNEUROSCI.4178-05.2006.
ieee: Á. Kulik et al., “Compartment-dependent colocalization of Kir3.2-containing
K+ channels and GABAB receptors in hippocampal pyramidal cells,” Journal of
Neuroscience, vol. 26, no. 16. Society for Neuroscience, pp. 4289–4297, 2006.
ista: Kulik Á, Vida I, Fukazawa Y, Guetg N, Kasugai Y, Marker C, Rigato F, Bettler
B, Wickman K, Frotscher M, Shigemoto R. 2006. Compartment-dependent colocalization
of Kir3.2-containing K+ channels and GABAB receptors in hippocampal pyramidal
cells. Journal of Neuroscience. 26(16), 4289–4297.
mla: Kulik, Ákos, et al. “Compartment-Dependent Colocalization of Kir3.2-Containing
K+ Channels and GABAB Receptors in Hippocampal Pyramidal Cells.” Journal of
Neuroscience, vol. 26, no. 16, Society for Neuroscience, 2006, pp. 4289–97,
doi:10.1523/JNEUROSCI.4178-05.2006.
short: Á. Kulik, I. Vida, Y. Fukazawa, N. Guetg, Y. Kasugai, C. Marker, F. Rigato,
B. Bettler, K. Wickman, M. Frotscher, R. Shigemoto, Journal of Neuroscience 26
(2006) 4289–4297.
date_created: 2018-12-11T11:58:56Z
date_published: 2006-04-19T00:00:00Z
date_updated: 2021-01-12T06:58:54Z
day: '19'
doi: 10.1523/JNEUROSCI.4178-05.2006
extern: 1
intvolume: ' 26'
issue: '16'
month: '04'
page: 4289 - 4297
publication: Journal of Neuroscience
publication_status: published
publisher: Society for Neuroscience
publist_id: '4235'
quality_controlled: 0
status: public
title: Compartment-dependent colocalization of Kir3.2-containing K+ channels and GABAB
receptors in hippocampal pyramidal cells
type: journal_article
volume: 26
year: '2006'
...
---
_id: '2663'
abstract:
- lang: eng
text: The rocker mice are hereditary ataxic mutants that carry a point mutation
in the gene encoding the CaV2.1 (P/Q-type) Ca2+ channel α1 subunit, and show the
mildest symptoms among the reported CaV2.1 mutant mice. We studied the basic characteristics
of the rocker mutant Ca2+ channel and their impacts on excitatory synaptic transmission
in cerebellar Purkinje cells (PCs). In acutely dissociated PC somas, the rocker
mutant channel showed a moderate reduction in Ca2+ channel current density, whereas
its kinetics and voltage dependency of gating remained nearly normal. Despite
the small changes in channel function, synaptic transmission in the parallel fiber
(PF)-PC synapses was severely impaired. The climbing fiber inputs onto PCs showed
a moderate impairment but could elicit normal complex spikes. Presynaptic function
of the PF-PC synapses, however, was unexpectedly almost normal in terms of paired-pulse
facilitation, sensitivity to extracellular Ca2+ concentration and glutamate concentration
in synaptic clefts. Electron microscopic analyses including freeze-fracture replica
labeling revealed that both the number and density of postsynaptic α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic
acid (AMPA) receptors substantially decreased without gross structural changes
of the PF-PC synapses. We also observed an abnormal arborization of PC dendrites
in young adult rocker mice (∼ 1 month old). These lines of evidence suggest that
even a moderate dysfunction of CaV2.1 Ca2+ channel can cause substantial changes
in postsynaptic molecular composition of the PF-PC synapses and dendritic structure
of PCs.
author:
- first_name: Takashi
full_name: Kodama, Takashi
last_name: Kodama
- first_name: Yuko
full_name: Itsukaichi-Nishida, Yuko
last_name: Itsukaichi Nishida
- first_name: Yugo
full_name: Fukazawa, Yugo
last_name: Fukazawa
- first_name: Minoru
full_name: Wakamori, Minoru
last_name: Wakamori
- first_name: Mariko
full_name: Miyata, Mariko
last_name: Miyata
- first_name: Elek
full_name: Molnár, Elek
last_name: Molnár
- first_name: Yasuo
full_name: Mori, Yasuo
last_name: Mori
- first_name: Ryuichi
full_name: Ryuichi Shigemoto
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Keiji
full_name: Imoto, Keiji
last_name: Imoto
citation:
ama: Kodama T, Itsukaichi Nishida Y, Fukazawa Y, et al. A CaV2.1 calcium channel
mutation rocker reduces the number of postsynaptic AMPA receptors in parallel
fiber-Purkinje cell synapses. European Journal of Neuroscience. 2006;24(11):2993-3007.
doi:10.1111/j.1460-9568.2006.05191.x
apa: Kodama, T., Itsukaichi Nishida, Y., Fukazawa, Y., Wakamori, M., Miyata, M.,
Molnár, E., … Imoto, K. (2006). A CaV2.1 calcium channel mutation rocker reduces
the number of postsynaptic AMPA receptors in parallel fiber-Purkinje cell synapses.
European Journal of Neuroscience. Wiley-Blackwell. https://doi.org/10.1111/j.1460-9568.2006.05191.x
chicago: Kodama, Takashi, Yuko Itsukaichi Nishida, Yugo Fukazawa, Minoru Wakamori,
Mariko Miyata, Elek Molnár, Yasuo Mori, Ryuichi Shigemoto, and Keiji Imoto. “A
CaV2.1 Calcium Channel Mutation Rocker Reduces the Number of Postsynaptic AMPA
Receptors in Parallel Fiber-Purkinje Cell Synapses.” European Journal of Neuroscience.
Wiley-Blackwell, 2006. https://doi.org/10.1111/j.1460-9568.2006.05191.x.
ieee: T. Kodama et al., “A CaV2.1 calcium channel mutation rocker reduces
the number of postsynaptic AMPA receptors in parallel fiber-Purkinje cell synapses,”
European Journal of Neuroscience, vol. 24, no. 11. Wiley-Blackwell, pp.
2993–3007, 2006.
ista: Kodama T, Itsukaichi Nishida Y, Fukazawa Y, Wakamori M, Miyata M, Molnár E,
Mori Y, Shigemoto R, Imoto K. 2006. A CaV2.1 calcium channel mutation rocker reduces
the number of postsynaptic AMPA receptors in parallel fiber-Purkinje cell synapses.
European Journal of Neuroscience. 24(11), 2993–3007.
mla: Kodama, Takashi, et al. “A CaV2.1 Calcium Channel Mutation Rocker Reduces the
Number of Postsynaptic AMPA Receptors in Parallel Fiber-Purkinje Cell Synapses.”
European Journal of Neuroscience, vol. 24, no. 11, Wiley-Blackwell, 2006,
pp. 2993–3007, doi:10.1111/j.1460-9568.2006.05191.x.
short: T. Kodama, Y. Itsukaichi Nishida, Y. Fukazawa, M. Wakamori, M. Miyata, E.
Molnár, Y. Mori, R. Shigemoto, K. Imoto, European Journal of Neuroscience 24 (2006)
2993–3007.
date_created: 2018-12-11T11:58:56Z
date_published: 2006-12-01T00:00:00Z
date_updated: 2021-01-12T06:58:54Z
day: '01'
doi: 10.1111/j.1460-9568.2006.05191.x
extern: 1
intvolume: ' 24'
issue: '11'
month: '12'
page: 2993 - 3007
publication: European Journal of Neuroscience
publication_status: published
publisher: Wiley-Blackwell
publist_id: '4233'
quality_controlled: 0
status: public
title: A CaV2.1 calcium channel mutation rocker reduces the number of postsynaptic
AMPA receptors in parallel fiber-Purkinje cell synapses
type: journal_article
volume: 24
year: '2006'
...
---
_id: '2664'
abstract:
- lang: eng
text: Metabotropic glutamate receptors (mGlus) are a family of G-protein-coupled
receptors activated by the neurotransmitter glutamate. Molecular cloning has revealed
eight different subtypes (mGlu1-8) with distinct molecular and pharmacological
properties. Multiplicity in this receptor family is further generated through
alternative splicing. mGlus activate a multitude of signalling pathways important
for modulating neuronal excitability, synaptic plasticity and feedback regulation
of neurotransmitter release. In this review, we summarize anatomical findings
(from our work and that of other laboratories) describing their distribution in
the central nervous system. Recent evidence regarding the localization of these
receptors in peripheral tissues will also be examined. The distinct regional,
cellular and subcellular distribution of mGlus in the brain will be discussed
in view of their relationship to neurotransmitter release sites and of possible
functional implications.
author:
- first_name: Francesco
full_name: Ferraguti, Francesco
last_name: Ferraguti
- first_name: Ryuichi
full_name: Ryuichi Shigemoto
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
citation:
ama: Ferraguti F, Shigemoto R. Metabotropic glutamate receptors. Cell and Tissue
Research. 2006;326(2):483-504. doi:10.1007/s00441-006-0266-5
apa: Ferraguti, F., & Shigemoto, R. (2006). Metabotropic glutamate receptors.
Cell and Tissue Research. Springer. https://doi.org/10.1007/s00441-006-0266-5
chicago: Ferraguti, Francesco, and Ryuichi Shigemoto. “Metabotropic Glutamate Receptors.”
Cell and Tissue Research. Springer, 2006. https://doi.org/10.1007/s00441-006-0266-5.
ieee: F. Ferraguti and R. Shigemoto, “Metabotropic glutamate receptors,” Cell
and Tissue Research, vol. 326, no. 2. Springer, pp. 483–504, 2006.
ista: Ferraguti F, Shigemoto R. 2006. Metabotropic glutamate receptors. Cell and
Tissue Research. 326(2), 483–504.
mla: Ferraguti, Francesco, and Ryuichi Shigemoto. “Metabotropic Glutamate Receptors.”
Cell and Tissue Research, vol. 326, no. 2, Springer, 2006, pp. 483–504,
doi:10.1007/s00441-006-0266-5.
short: F. Ferraguti, R. Shigemoto, Cell and Tissue Research 326 (2006) 483–504.
date_created: 2018-12-11T11:58:57Z
date_published: 2006-11-01T00:00:00Z
date_updated: 2020-07-14T12:45:44Z
day: '01'
doi: 10.1007/s00441-006-0266-5
extern: 1
intvolume: ' 326'
issue: '2'
month: '11'
page: 483 - 504
publication: Cell and Tissue Research
publication_status: published
publisher: Springer
publist_id: '4234'
quality_controlled: 0
status: public
title: Metabotropic glutamate receptors
type: review
volume: 326
year: '2006'
...
---
_id: '2745'
abstract:
- lang: eng
text: 'We consider the dynamics of N boson systems interacting through a pair potential
N -1 V a (x i -x j ) where V a (x)=a -3 V(x/a). We denote the solution to the
N-particle Schrödinger equation by Ψ N, t . Recall that the Gross-Pitaevskii (GP)
equation is a nonlinear Schrödinger equation and the GP hierarchy is an infinite
BBGKY hierarchy of equations so that if u t solves the GP equation, then the family
of k-particle density matrices [InlineMediaObject not available: see fulltext.]
solves the GP hierarchy. Under the assumption that a = Nε for 0 < ε < 3/5,
we prove that as N→∞ the limit points of the k-particle density matrices of Ψ
N, t are solutions of the GP hierarchy with the coupling constant in the nonlinear
term of the GP equation given by ∫ V (x)dx. The uniqueness of the solutions of
this hierarchy remains an open question.'
author:
- first_name: Alexander
full_name: Elgart, Alexander
last_name: Elgart
- first_name: László
full_name: László Erdös
id: 4DBD5372-F248-11E8-B48F-1D18A9856A87
last_name: Erdös
orcid: 0000-0001-5366-9603
- first_name: Benjamin
full_name: Schlein, Benjamin
last_name: Schlein
- first_name: Horng
full_name: Yau, Horng-Tzer
last_name: Yau
citation:
ama: Elgart A, Erdös L, Schlein B, Yau H. Gross-Pitaevskii equation as the mean
field limit of weakly coupled bosons. Archive for Rational Mechanics and Analysis.
2006;179(2):265-283. doi:10.1007/s00205-005-0388-z
apa: Elgart, A., Erdös, L., Schlein, B., & Yau, H. (2006). Gross-Pitaevskii
equation as the mean field limit of weakly coupled bosons. Archive for Rational
Mechanics and Analysis. Springer. https://doi.org/10.1007/s00205-005-0388-z
chicago: Elgart, Alexander, László Erdös, Benjamin Schlein, and Horng Yau. “Gross-Pitaevskii
Equation as the Mean Field Limit of Weakly Coupled Bosons.” Archive for Rational
Mechanics and Analysis. Springer, 2006. https://doi.org/10.1007/s00205-005-0388-z.
ieee: A. Elgart, L. Erdös, B. Schlein, and H. Yau, “Gross-Pitaevskii equation as
the mean field limit of weakly coupled bosons,” Archive for Rational Mechanics
and Analysis, vol. 179, no. 2. Springer, pp. 265–283, 2006.
ista: Elgart A, Erdös L, Schlein B, Yau H. 2006. Gross-Pitaevskii equation as the
mean field limit of weakly coupled bosons. Archive for Rational Mechanics and
Analysis. 179(2), 265–283.
mla: Elgart, Alexander, et al. “Gross-Pitaevskii Equation as the Mean Field Limit
of Weakly Coupled Bosons.” Archive for Rational Mechanics and Analysis,
vol. 179, no. 2, Springer, 2006, pp. 265–83, doi:10.1007/s00205-005-0388-z.
short: A. Elgart, L. Erdös, B. Schlein, H. Yau, Archive for Rational Mechanics and
Analysis 179 (2006) 265–283.
date_created: 2018-12-11T11:59:22Z
date_published: 2006-02-01T00:00:00Z
date_updated: 2021-01-12T06:59:25Z
day: '01'
doi: 10.1007/s00205-005-0388-z
extern: 1
intvolume: ' 179'
issue: '2'
month: '02'
page: 265 - 283
publication: Archive for Rational Mechanics and Analysis
publication_status: published
publisher: Springer
publist_id: '4147'
quality_controlled: 0
status: public
title: Gross-Pitaevskii equation as the mean field limit of weakly coupled bosons
type: journal_article
volume: 179
year: '2006'
...
---
_id: '2746'
abstract:
- lang: eng
text: We consider random Schrödinger equations on Rd or Zd for d ≥ 3 with uncorrelated,
identically distributed random potential. Denote by λ the coupling constant and
ψt the solution with initial data ψ0.
alternative_title:
- LNP
author:
- first_name: László
full_name: László Erdös
id: 4DBD5372-F248-11E8-B48F-1D18A9856A87
last_name: Erdös
orcid: 0000-0001-5366-9603
- first_name: Manfred
full_name: Salmhofer, Manfred
last_name: Salmhofer
- first_name: Horng
full_name: Yau, Horng-Tzer
last_name: Yau
citation:
ama: 'Erdös L, Salmhofer M, Yau H. Towards the quantum Brownian motion. In: Vol
690. World Scientific Publishing; 2006:233-257. doi:10.1007/3-540-34273-7_18'
apa: 'Erdös, L., Salmhofer, M., & Yau, H. (2006). Towards the quantum Brownian
motion (Vol. 690, pp. 233–257). Presented at the QMath: Mathematical Results in
Quantum Physics, World Scientific Publishing. https://doi.org/10.1007/3-540-34273-7_18'
chicago: Erdös, László, Manfred Salmhofer, and Horng Yau. “Towards the Quantum Brownian
Motion,” 690:233–57. World Scientific Publishing, 2006. https://doi.org/10.1007/3-540-34273-7_18.
ieee: 'L. Erdös, M. Salmhofer, and H. Yau, “Towards the quantum Brownian motion,”
presented at the QMath: Mathematical Results in Quantum Physics, 2006, vol. 690,
pp. 233–257.'
ista: 'Erdös L, Salmhofer M, Yau H. 2006. Towards the quantum Brownian motion. QMath:
Mathematical Results in Quantum Physics, LNP, vol. 690, 233–257.'
mla: Erdös, László, et al. Towards the Quantum Brownian Motion. Vol. 690,
World Scientific Publishing, 2006, pp. 233–57, doi:10.1007/3-540-34273-7_18.
short: L. Erdös, M. Salmhofer, H. Yau, in:, World Scientific Publishing, 2006, pp.
233–257.
conference:
name: 'QMath: Mathematical Results in Quantum Physics'
date_created: 2018-12-11T11:59:23Z
date_published: 2006-01-01T00:00:00Z
date_updated: 2021-01-12T06:59:25Z
day: '01'
doi: 10.1007/3-540-34273-7_18
extern: 1
intvolume: ' 690'
month: '01'
page: 233 - 257
publication_status: published
publisher: World Scientific Publishing
publist_id: '4146'
quality_controlled: 0
status: public
title: Towards the quantum Brownian motion
type: conference
volume: 690
year: '2006'
...
---
_id: '2747'
abstract:
- lang: eng
text: Consider a system of N bosons on the three-dimensional unit torus interacting
via a pair potential N 2V(N(x i - x j)) where x = (x i, . . ., x N) denotes the
positions of the particles. Suppose that the initial data ψ N,0 satisfies the
condition 〈ψ N,0, H 2 Nψ N,0) ≤ C N 2 where H N is the Hamiltonian of the Bose
system. This condition is satisfied if ψ N,0 = W Nφ N,t where W N is an approximate
ground state to H N and φ N,0 is regular. Let ψ N,t denote the solution to the
Schrödinger equation with Hamiltonian H N. Gross and Pitaevskii proposed to model
the dynamics of such a system by a nonlinear Schrödinger equation, the Gross-Pitaevskii
(GP) equation. The GP hierarchy is an infinite BBGKY hierarchy of equations so
that if u t solves the GP equation, then the family of k-particle density matrices
⊗ k |u t?〉 〈 t | solves the GP hierarchy. We prove that as N → ∞ the limit points
of the k-particle density matrices of ψ N,t are solutions of the GP hierarchy.
Our analysis requires that the N-boson dynamics be described by a modified Hamiltonian
that cuts off the pair interactions whenever at least three particles come into
a region with diameter much smaller than the typical interparticle distance. Our
proof can be extended to a modified Hamiltonian that only forbids at least n particles
from coming close together for any fixed n.
author:
- first_name: László
full_name: László Erdös
id: 4DBD5372-F248-11E8-B48F-1D18A9856A87
last_name: Erdös
orcid: 0000-0001-5366-9603
- first_name: Benjamin
full_name: Schlein, Benjamin
last_name: Schlein
- first_name: Horng
full_name: Yau, Horng-Tzer
last_name: Yau
citation:
ama: Erdös L, Schlein B, Yau H. Derivation of the Gross-Pitaevskii hierarchy for
the dynamics of Bose-Einstein condensate. Communications on Pure and Applied
Mathematics. 2006;59(12):1659-1741. doi:10.1002/cpa.20123
apa: Erdös, L., Schlein, B., & Yau, H. (2006). Derivation of the Gross-Pitaevskii
hierarchy for the dynamics of Bose-Einstein condensate. Communications on Pure
and Applied Mathematics. Wiley-Blackwell. https://doi.org/10.1002/cpa.20123
chicago: Erdös, László, Benjamin Schlein, and Horng Yau. “Derivation of the Gross-Pitaevskii
Hierarchy for the Dynamics of Bose-Einstein Condensate.” Communications on
Pure and Applied Mathematics. Wiley-Blackwell, 2006. https://doi.org/10.1002/cpa.20123.
ieee: L. Erdös, B. Schlein, and H. Yau, “Derivation of the Gross-Pitaevskii hierarchy
for the dynamics of Bose-Einstein condensate,” Communications on Pure and Applied
Mathematics, vol. 59, no. 12. Wiley-Blackwell, pp. 1659–1741, 2006.
ista: Erdös L, Schlein B, Yau H. 2006. Derivation of the Gross-Pitaevskii hierarchy
for the dynamics of Bose-Einstein condensate. Communications on Pure and Applied
Mathematics. 59(12), 1659–1741.
mla: Erdös, László, et al. “Derivation of the Gross-Pitaevskii Hierarchy for the
Dynamics of Bose-Einstein Condensate.” Communications on Pure and Applied Mathematics,
vol. 59, no. 12, Wiley-Blackwell, 2006, pp. 1659–741, doi:10.1002/cpa.20123.
short: L. Erdös, B. Schlein, H. Yau, Communications on Pure and Applied Mathematics
59 (2006) 1659–1741.
date_created: 2018-12-11T11:59:23Z
date_published: 2006-12-01T00:00:00Z
date_updated: 2021-01-12T06:59:26Z
day: '01'
doi: 10.1002/cpa.20123
extern: 1
intvolume: ' 59'
issue: '12'
month: '12'
page: 1659 - 1741
publication: Communications on Pure and Applied Mathematics
publication_status: published
publisher: Wiley-Blackwell
publist_id: '4145'
quality_controlled: 0
status: public
title: Derivation of the Gross-Pitaevskii hierarchy for the dynamics of Bose-Einstein
condensate
type: journal_article
volume: 59
year: '2006'
...
---
_id: '2791'
abstract:
- lang: eng
text: Generally, the motion of fluids is smooth and laminar at low speeds but becomes
highly disordered and turbulent as the velocity increases. The transition from
laminar to turbulent flow can involve a sequence of instabilities in which the
system realizes progressively more complicated states, or it can occur suddenly.
Once the transition has taken place, it is generally assumed that, under steady
conditions, the turbulent state will persist indefinitely. The flow of a fluid
down a straight pipe provides a ubiquitous example of a shear flow undergoing
a sudden transition from laminar to turbulent motion. Extensive calculations and
experimental studies have shown that, at relatively low flow rates, turbulence
in pipes is transient, and is characterized by an exponential distribution of
lifetimes. They also suggest that for Reynolds numbers exceeding a critical value
the lifetime diverges (that is, becomes infinitely large), marking a change from
transient to persistent turbulence. Here we present experimental data and numerical
calculations covering more than two decades of lifetimes, showing that the lifetime
does not in fact diverge but rather increases exponentially with the Reynolds
number. This implies that turbulence in pipes is only a transient event (contrary
to the commonly accepted view), and that the turbulent and laminar states remain
dynamically connected, suggesting avenues for turbulence control.
author:
- first_name: Björn
full_name: Björn Hof
id: 3A374330-F248-11E8-B48F-1D18A9856A87
last_name: Hof
orcid: 0000-0003-2057-2754
- first_name: Jerry
full_name: Westerweel, Jerry
last_name: Westerweel
- first_name: Tobias
full_name: Schneider, Tobias M
last_name: Schneider
- first_name: Bruno
full_name: Eckhardt, Bruno
last_name: Eckhardt
citation:
ama: Hof B, Westerweel J, Schneider T, Eckhardt B. Finite lifetime of turbulence
in shear flows. Nature. 2006;443(7107):59-62. doi:10.1038/nature05089
apa: Hof, B., Westerweel, J., Schneider, T., & Eckhardt, B. (2006). Finite lifetime
of turbulence in shear flows. Nature. Nature Publishing Group. https://doi.org/10.1038/nature05089
chicago: Hof, Björn, Jerry Westerweel, Tobias Schneider, and Bruno Eckhardt. “Finite
Lifetime of Turbulence in Shear Flows.” Nature. Nature Publishing Group,
2006. https://doi.org/10.1038/nature05089.
ieee: B. Hof, J. Westerweel, T. Schneider, and B. Eckhardt, “Finite lifetime of
turbulence in shear flows,” Nature, vol. 443, no. 7107. Nature Publishing
Group, pp. 59–62, 2006.
ista: Hof B, Westerweel J, Schneider T, Eckhardt B. 2006. Finite lifetime of turbulence
in shear flows. Nature. 443(7107), 59–62.
mla: Hof, Björn, et al. “Finite Lifetime of Turbulence in Shear Flows.” Nature,
vol. 443, no. 7107, Nature Publishing Group, 2006, pp. 59–62, doi:10.1038/nature05089.
short: B. Hof, J. Westerweel, T. Schneider, B. Eckhardt, Nature 443 (2006) 59–62.
date_created: 2018-12-11T11:59:37Z
date_published: 2006-09-07T00:00:00Z
date_updated: 2021-01-12T06:59:44Z
day: '07'
doi: 10.1038/nature05089
extern: 1
intvolume: ' 443'
issue: '7107'
month: '09'
page: 59 - 62
publication: Nature
publication_status: published
publisher: Nature Publishing Group
publist_id: '4098'
quality_controlled: 0
status: public
title: Finite lifetime of turbulence in shear flows
type: journal_article
volume: 443
year: '2006'
...
---
_id: '2792'
abstract:
- lang: eng
text: Transition to turbulence in pipe flow has posed a riddle in fluid dynamics
since the pioneering experiments of Reynolds[1]. Although the laminar flow is
linearly stable for all flow rates, practical pipe flows become turbulent at large
enough flow speeds. Turbulence arises suddenly and fully without distinct steps
and without a clear critical point. The complexity of this problem has puzzled
mathematicians, physicists and engineers for more than a century and no satisfactory
explanation of this problem has been given. In a very recent theoretical approach
it has been suggested that unstable solutions of the Navier Stokes equations may
hold the key to understanding this problem. In numerical studies such unstable
states have been identified as exact solutions for the idealized case of a pipe
with periodic boundary conditions[2, 3]. These solutions have the form of waves
extending through the entire pipe and travelling in the streamwise direction at
a phase speed close to the bulk velocity of the fluid. With the aid of a recently
developed high-speed stereoscopic Particle Image Velocimetry (PIV) system, we
were able to observe transients of such unstable solutions in turbulent pipe flow[4].
author:
- first_name: Björn
full_name: Björn Hof
id: 3A374330-F248-11E8-B48F-1D18A9856A87
last_name: Hof
orcid: 0000-0003-2057-2754
- first_name: Casimir
full_name: van Doorne, Casimir W
last_name: Van Doorne
- first_name: Jerry
full_name: Westerweel, Jerry
last_name: Westerweel
- first_name: Frans
full_name: Nieuwstadt, Frans T
last_name: Nieuwstadt
citation:
ama: Hof B, Van Doorne C, Westerweel J, Nieuwstadt F. Observation of nonlinear travelling
waves in turbulent pipe flow. Fluid Mechanics and its Applications. 2006;78:109-114.
doi:10.1007/1-4020-4159-4_11
apa: Hof, B., Van Doorne, C., Westerweel, J., & Nieuwstadt, F. (2006). Observation
of nonlinear travelling waves in turbulent pipe flow. Fluid Mechanics and Its
Applications. Springer. https://doi.org/10.1007/1-4020-4159-4_11
chicago: Hof, Björn, Casimir Van Doorne, Jerry Westerweel, and Frans Nieuwstadt.
“Observation of Nonlinear Travelling Waves in Turbulent Pipe Flow.” Fluid Mechanics
and Its Applications. Springer, 2006. https://doi.org/10.1007/1-4020-4159-4_11.
ieee: B. Hof, C. Van Doorne, J. Westerweel, and F. Nieuwstadt, “Observation of nonlinear
travelling waves in turbulent pipe flow,” Fluid Mechanics and its Applications,
vol. 78. Springer, pp. 109–114, 2006.
ista: Hof B, Van Doorne C, Westerweel J, Nieuwstadt F. 2006. Observation of nonlinear
travelling waves in turbulent pipe flow. Fluid Mechanics and its Applications.
78, 109–114.
mla: Hof, Björn, et al. “Observation of Nonlinear Travelling Waves in Turbulent
Pipe Flow.” Fluid Mechanics and Its Applications, vol. 78, Springer, 2006,
pp. 109–14, doi:10.1007/1-4020-4159-4_11.
short: B. Hof, C. Van Doorne, J. Westerweel, F. Nieuwstadt, Fluid Mechanics and
Its Applications 78 (2006) 109–114.
date_created: 2018-12-11T11:59:37Z
date_published: 2006-01-18T00:00:00Z
date_updated: 2021-01-12T06:59:45Z
day: '18'
doi: 10.1007/1-4020-4159-4_11
extern: 1
intvolume: ' 78'
month: '01'
page: 109 - 114
publication: Fluid Mechanics and its Applications
publication_status: published
publisher: Springer
publist_id: '4097'
quality_controlled: 0
status: public
title: Observation of nonlinear travelling waves in turbulent pipe flow
type: journal_article
volume: 78
year: '2006'
...
---
_id: '2894'
abstract:
- lang: eng
text: IL-10 is a potent anti-inflammatory and immunomodulatory cytokine, exerting
major effects in the degree and quality of the immune response. Using a newly
generated IL-10 reporter mouse model, which easily allows the study of IL-10 expression
from each allele in a single cell, we report here for the first time that IL-10
is predominantly monoallelic expressed in CD4+ T cells. Furthermore, we have compelling
evidence that this expression pattern is not due to parental imprinting, allelic
exclusion, or strong allelic bias. Instead, our results support a stochastic regulation
mechanism, in which the probability to initiate allelic transcription depends
on the strength of TCR signaling and subsequent capacity to overcome restrictions
imposed by chromatin hypoacetylation. In vivo Ag-experienced T cells show a higher
basal probability to transcribe IL-10 when compared with naive cells, yet still
show mostly monoallelic IL-10 expression. Finally, statistical analysis on allelic
expression data shows transcriptional independence between both alleles. We conclude
that CD4+ T cells have a low probability for IL-10 allelic activation resulting
in a predominantly monoallelic expression pattern, and that IL-10 expression appears
to be stochastically regulated by controlling the frequency of expressing cells,
rather than absolute protein levels per cell.
author:
- first_name: Dinis
full_name: Calado, Dinis P
last_name: Calado
- first_name: Tiago
full_name: Tiago Paixao
id: 2C5658E6-F248-11E8-B48F-1D18A9856A87
last_name: Paixao
orcid: 0000-0003-2361-3953
- first_name: Dan
full_name: Holmberg, Dan
last_name: Holmberg
- first_name: Matthias
full_name: Haury, Matthias
last_name: Haury
citation:
ama: Calado D, Paixao T, Holmberg D, Haury M. Stochastic Monoallelic Expression
of IL 10 in T Cells. Journal of Immunology. 2006;177(8):5358-5364. doi:10.4049/jimmunol.177.8.5358
apa: Calado, D., Paixao, T., Holmberg, D., & Haury, M. (2006). Stochastic Monoallelic
Expression of IL 10 in T Cells. Journal of Immunology. American Association
of Immunologists. https://doi.org/10.4049/jimmunol.177.8.5358
chicago: Calado, Dinis, Tiago Paixao, Dan Holmberg, and Matthias Haury. “Stochastic
Monoallelic Expression of IL 10 in T Cells.” Journal of Immunology. American
Association of Immunologists, 2006. https://doi.org/10.4049/jimmunol.177.8.5358 .
ieee: D. Calado, T. Paixao, D. Holmberg, and M. Haury, “Stochastic Monoallelic Expression
of IL 10 in T Cells,” Journal of Immunology, vol. 177, no. 8. American
Association of Immunologists, pp. 5358–5364, 2006.
ista: Calado D, Paixao T, Holmberg D, Haury M. 2006. Stochastic Monoallelic Expression
of IL 10 in T Cells. Journal of Immunology. 177(8), 5358–5364.
mla: Calado, Dinis, et al. “Stochastic Monoallelic Expression of IL 10 in T Cells.”
Journal of Immunology, vol. 177, no. 8, American Association of Immunologists,
2006, pp. 5358–64, doi:10.4049/jimmunol.177.8.5358
.
short: D. Calado, T. Paixao, D. Holmberg, M. Haury, Journal of Immunology 177 (2006)
5358–5364.
date_created: 2018-12-11T12:00:11Z
date_published: 2006-01-01T00:00:00Z
date_updated: 2021-01-12T07:00:32Z
day: '01'
doi: '10.4049/jimmunol.177.8.5358 '
extern: 1
intvolume: ' 177'
issue: '8'
month: '01'
page: 5358 - 5364
publication: Journal of Immunology
publication_status: published
publisher: American Association of Immunologists
publist_id: '3864'
quality_controlled: 0
status: public
title: Stochastic Monoallelic Expression of IL 10 in T Cells
type: journal_article
volume: 177
year: '2006'
...
---
_id: '2921'
abstract:
- lang: eng
text: Most binocular stereo algorithms assume that all scene elements are visible
from both cameras. Scene elements that are visible from only one camera, known
as occlusions, pose an important challenge for stereo. Occlusions are important
for segmentation, because they appear near discontinuities. However, stereo algorithms
tend to ignore occlusions because of their difficulty. One reason is that occlusions
require the input images to be treated symmetrically, which complicates the problem
formulation. Worse, certain depth maps imply physically impossible scene configurations,
and must be excluded from the output. In this chapter we approach the problem
of binocular stereo with occlusions from an energy minimization viewpoint. We
begin by reviewing traditional stereo methods that do not handle occlusions. If
occlusions are ignored, it is easy to formulate the stereo problem as a pixel
labeling problem, which leads to an energy function that is common in early vision.
This kind of energy function can he minimized using graph cuts, which is a combinatorial
optimization technique that has proven to be very effective for low-level vision
problems. Motivated by this, we have designed two graph cut stereo algorithms
that are designed to handle occlusions. These algorithms produce promising experimental
results on real data with ground truth.
author:
- first_name: Vladimir
full_name: Vladimir Kolmogorov
id: 3D50B0BA-F248-11E8-B48F-1D18A9856A87
last_name: Kolmogorov
- first_name: Ramin
full_name: Zabih, Ramin
last_name: Zabih
citation:
ama: 'Kolmogorov V, Zabih R. Graph cut algorithms for binocular stereo with occlusions.
In: Handbook of Mathematical Models in Computer Vision. Springer; 2006:423-427.
doi:10.1007/0-387-28831-7_26'
apa: Kolmogorov, V., & Zabih, R. (2006). Graph cut algorithms for binocular
stereo with occlusions. In Handbook of Mathematical Models in Computer Vision
(pp. 423–427). Springer. https://doi.org/10.1007/0-387-28831-7_26
chicago: Kolmogorov, Vladimir, and Ramin Zabih. “Graph Cut Algorithms for Binocular
Stereo with Occlusions.” In Handbook of Mathematical Models in Computer Vision,
423–27. Springer, 2006. https://doi.org/10.1007/0-387-28831-7_26.
ieee: V. Kolmogorov and R. Zabih, “Graph cut algorithms for binocular stereo with
occlusions,” in Handbook of Mathematical Models in Computer Vision, Springer,
2006, pp. 423–427.
ista: 'Kolmogorov V, Zabih R. 2006.Graph cut algorithms for binocular stereo with
occlusions. In: Handbook of Mathematical Models in Computer Vision. , 423–427.'
mla: Kolmogorov, Vladimir, and Ramin Zabih. “Graph Cut Algorithms for Binocular
Stereo with Occlusions.” Handbook of Mathematical Models in Computer Vision,
Springer, 2006, pp. 423–27, doi:10.1007/0-387-28831-7_26.
short: V. Kolmogorov, R. Zabih, in:, Handbook of Mathematical Models in Computer
Vision, Springer, 2006, pp. 423–427.
date_created: 2018-12-11T12:00:21Z
date_published: 2006-01-01T00:00:00Z
date_updated: 2021-01-12T07:00:42Z
day: '01'
doi: 10.1007/0-387-28831-7_26
extern: 1
month: '01'
page: 423 - 427
publication: Handbook of Mathematical Models in Computer Vision
publication_status: published
publisher: Springer
publist_id: '3816'
quality_controlled: 0
status: public
title: Graph cut algorithms for binocular stereo with occlusions
type: book_chapter
year: '2006'
...
---
_id: '3002'
abstract:
- lang: eng
text: Arabidopsis thaliana is currently the most important model organism for basic
molecular plant research. It is also a favourable model for developmental biology,
as its embryogenesis follows a nearly invariant pattern of cell divisions and
cell type specifications. Study of embryogenesis can involve genetic, physiological
or biochemical approaches, but is always limited by the inaccessibility of the
embryos which develop deep inside maternal tissue. Thus, for developmental studies,
there is an increasing demand for methods which allow embryogenesis under artificial
conditions, providing better accessibility to experimental manipulation. In this
chapter, we address theoretical aspects of embryo culture, give some thoughts
on which embryo culture system is suited best for which application and finally
discuss three current methods which have been successfully used in Arabidopsis
embryo culture. © 2006 Springer-Verlag Berlin Heidelberg.
alternative_title:
- Plant Cell Monographs
author:
- first_name: Michael
full_name: Sauer, Michael
last_name: Sauer
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: 'Sauer M, Friml J. In vitro culture of Arabidopsis embryos. In: Mujib A, Šamaj
J, eds. Somatic Embryogenesis. Vol 2. Springer; 2006:343-354. doi:10.1007/7089_020'
apa: Sauer, M., & Friml, J. (2006). In vitro culture of Arabidopsis embryos.
In A. Mujib & J. Šamaj (Eds.), Somatic Embryogenesis (Vol. 2, pp. 343–354).
Springer. https://doi.org/10.1007/7089_020
chicago: Sauer, Michael, and Jiří Friml. “In Vitro Culture of Arabidopsis Embryos.”
In Somatic Embryogenesis, edited by Abdul Mujib and Jozef Šamaj, 2:343–54.
Springer, 2006. https://doi.org/10.1007/7089_020.
ieee: M. Sauer and J. Friml, “In vitro culture of Arabidopsis embryos,” in Somatic
Embryogenesis, vol. 2, A. Mujib and J. Šamaj, Eds. Springer, 2006, pp. 343–354.
ista: 'Sauer M, Friml J. 2006.In vitro culture of Arabidopsis embryos. In: Somatic
Embryogenesis. Plant Cell Monographs, vol. 2, 343–354.'
mla: Sauer, Michael, and Jiří Friml. “In Vitro Culture of Arabidopsis Embryos.”
Somatic Embryogenesis, edited by Abdul Mujib and Jozef Šamaj, vol. 2, Springer,
2006, pp. 343–54, doi:10.1007/7089_020.
short: M. Sauer, J. Friml, in:, A. Mujib, J. Šamaj (Eds.), Somatic Embryogenesis,
Springer, 2006, pp. 343–354.
date_created: 2018-12-11T12:00:48Z
date_published: 2006-01-01T00:00:00Z
date_updated: 2021-01-12T07:40:23Z
day: '01'
doi: 10.1007/7089_020
editor:
- first_name: Abdul
full_name: Mujib, Abdul
last_name: Mujib
- first_name: Jozef
full_name: Šamaj, Jozef
last_name: Šamaj
extern: 1
intvolume: ' 2'
month: '01'
page: 343 - 354
publication: Somatic Embryogenesis
publication_status: published
publisher: Springer
publist_id: '3699'
quality_controlled: 0
status: public
title: In vitro culture of Arabidopsis embryos
type: book_chapter
volume: 2
year: '2006'
...
---
_id: '3005'
author:
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Philip
full_name: Benfey, Philip
last_name: Benfey
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Malcolm
full_name: Bennett, Malcolm
last_name: Bennett
- first_name: Thomas
full_name: Berleth, Thomas
last_name: Berleth
- first_name: Niko
full_name: Geldner, Niko
last_name: Geldner
- first_name: Markus
full_name: Grebe, Markus
last_name: Grebe
- first_name: Marcus
full_name: Heisler, Marcus
last_name: Heisler
- first_name: Jan
full_name: Hejátko, Jan
last_name: Hejátko
- first_name: Gerd
full_name: Jürgens, Gerd
last_name: Jürgens
- first_name: Thomas
full_name: Laux, Thomas
last_name: Laux
- first_name: Keith
full_name: Lindsey, Keith
last_name: Lindsey
- first_name: Wolfgang
full_name: Lukowitz, Wolfgang
last_name: Lukowitz
- first_name: Christian
full_name: Luschnig, Christian
last_name: Luschnig
- first_name: Remko
full_name: Offringa, Remko
last_name: Offringa
- first_name: Ben
full_name: Scheres, Ben
last_name: Scheres
- first_name: Ranjan
full_name: Swarup, Ranjan
last_name: Swarup
- first_name: Ramón
full_name: Torres Ruiz, Ramón
last_name: Torres Ruiz
- first_name: Dolf
full_name: Weijers, Dolf
last_name: Weijers
- first_name: Eva
full_name: Zažímalová, Eva
last_name: Zažímalová
citation:
ama: 'Friml J, Benfey P, Benková E, et al. Apical-basal polarity: Why plant cells
don’t stand on their heads. Trends in Plant Science. 2006;11(1):12-14.
doi:10.1016/j.tplants.2005.11.010'
apa: 'Friml, J., Benfey, P., Benková, E., Bennett, M., Berleth, T., Geldner, N.,
… Zažímalová, E. (2006). Apical-basal polarity: Why plant cells don’t stand on
their heads. Trends in Plant Science. Cell Press. https://doi.org/10.1016/j.tplants.2005.11.010'
chicago: 'Friml, Jiří, Philip Benfey, Eva Benková, Malcolm Bennett, Thomas Berleth,
Niko Geldner, Markus Grebe, et al. “Apical-Basal Polarity: Why Plant Cells Don’t
Stand on Their Heads.” Trends in Plant Science. Cell Press, 2006. https://doi.org/10.1016/j.tplants.2005.11.010.'
ieee: 'J. Friml et al., “Apical-basal polarity: Why plant cells don’t stand
on their heads,” Trends in Plant Science, vol. 11, no. 1. Cell Press, pp.
12–14, 2006.'
ista: 'Friml J, Benfey P, Benková E, Bennett M, Berleth T, Geldner N, Grebe M, Heisler
M, Hejátko J, Jürgens G, Laux T, Lindsey K, Lukowitz W, Luschnig C, Offringa R,
Scheres B, Swarup R, Torres Ruiz R, Weijers D, Zažímalová E. 2006. Apical-basal
polarity: Why plant cells don’t stand on their heads. Trends in Plant Science.
11(1), 12–14.'
mla: 'Friml, Jiří, et al. “Apical-Basal Polarity: Why Plant Cells Don’t Stand on
Their Heads.” Trends in Plant Science, vol. 11, no. 1, Cell Press, 2006,
pp. 12–14, doi:10.1016/j.tplants.2005.11.010.'
short: J. Friml, P. Benfey, E. Benková, M. Bennett, T. Berleth, N. Geldner, M. Grebe,
M. Heisler, J. Hejátko, G. Jürgens, T. Laux, K. Lindsey, W. Lukowitz, C. Luschnig,
R. Offringa, B. Scheres, R. Swarup, R. Torres Ruiz, D. Weijers, E. Zažímalová,
Trends in Plant Science 11 (2006) 12–14.
date_created: 2018-12-11T12:00:49Z
date_published: 2006-01-01T00:00:00Z
date_updated: 2021-01-12T07:40:24Z
day: '01'
doi: 10.1016/j.tplants.2005.11.010
extern: '1'
intvolume: ' 11'
issue: '1'
language:
- iso: eng
month: '01'
oa_version: None
page: 12 - 14
publication: Trends in Plant Science
publication_status: published
publisher: Cell Press
publist_id: '3697'
status: public
title: 'Apical-basal polarity: Why plant cells don''t stand on their heads'
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 11
year: '2006'
...
---
_id: '3006'
abstract:
- lang: eng
text: 'Dividing plant cells perform a remarkable task of building a new cell wall
within the cytoplasm in a few minutes. A long-standing paradigm claims that this
primordial cell wall, known as the cell plate, is generated by delivery of newly
synthesized material from Golgi apparatus-originated secretory vesicles. Here,
we show that, in diverse plant species, cell surface material, including plasma
membrane proteins, cell wall components, and exogenously applied endocytic tracers,
is rapidly delivered to the forming cell plate. Importantly, this occurs even
when de novo protein synthesis is blocked. In addition, cytokinesis-specific syntaxin
KNOLLE as well as plasma membrane (PM) resident proteins localize to endosomes
that fuse to initiate the cell plate. The rate of endocytosis is strongly enhanced
during cell plate formation, and its genetic or pharmacological inhibition leads
to cytokinesis defects. Our results reveal that endocytic delivery of cell surface
material significantly contributes to cell plate formation during plant cytokinesis. '
author:
- first_name: Pankaj
full_name: Dhonukshe, Pankaj
last_name: Dhonukshe
- first_name: František
full_name: Baluška, František
last_name: Baluška
- first_name: Markus
full_name: Schlicht, Markus
last_name: Schlicht
- first_name: Andrej
full_name: Hlavacka, Andrej
last_name: Hlavacka
- first_name: Jozef
full_name: Šamaj, Jozef
last_name: Šamaj
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Theodorus
full_name: Gadella, Theodorus W
last_name: Gadella
citation:
ama: Dhonukshe P, Baluška F, Schlicht M, et al. Endocytosis of cell surface material
mediates cell plate formation during plant cytokinesis. Developmental Cell.
2006;10(1):137-150. doi:10.1016/j.devcel.2005.11.015
apa: Dhonukshe, P., Baluška, F., Schlicht, M., Hlavacka, A., Šamaj, J., Friml, J.,
& Gadella, T. (2006). Endocytosis of cell surface material mediates cell plate
formation during plant cytokinesis. Developmental Cell. Cell Press. https://doi.org/10.1016/j.devcel.2005.11.015
chicago: Dhonukshe, Pankaj, František Baluška, Markus Schlicht, Andrej Hlavacka,
Jozef Šamaj, Jiří Friml, and Theodorus Gadella. “Endocytosis of Cell Surface Material
Mediates Cell Plate Formation during Plant Cytokinesis.” Developmental Cell.
Cell Press, 2006. https://doi.org/10.1016/j.devcel.2005.11.015.
ieee: P. Dhonukshe et al., “Endocytosis of cell surface material mediates
cell plate formation during plant cytokinesis,” Developmental Cell, vol.
10, no. 1. Cell Press, pp. 137–150, 2006.
ista: Dhonukshe P, Baluška F, Schlicht M, Hlavacka A, Šamaj J, Friml J, Gadella
T. 2006. Endocytosis of cell surface material mediates cell plate formation during
plant cytokinesis. Developmental Cell. 10(1), 137–150.
mla: Dhonukshe, Pankaj, et al. “Endocytosis of Cell Surface Material Mediates Cell
Plate Formation during Plant Cytokinesis.” Developmental Cell, vol. 10,
no. 1, Cell Press, 2006, pp. 137–50, doi:10.1016/j.devcel.2005.11.015.
short: P. Dhonukshe, F. Baluška, M. Schlicht, A. Hlavacka, J. Šamaj, J. Friml, T.
Gadella, Developmental Cell 10 (2006) 137–150.
date_created: 2018-12-11T12:00:49Z
date_published: 2006-01-01T00:00:00Z
date_updated: 2021-01-12T07:40:24Z
day: '01'
doi: 10.1016/j.devcel.2005.11.015
extern: 1
intvolume: ' 10'
issue: '1'
month: '01'
page: 137 - 150
publication: Developmental Cell
publication_status: published
publisher: Cell Press
publist_id: '3696'
quality_controlled: 0
status: public
title: Endocytosis of cell surface material mediates cell plate formation during plant
cytokinesis
type: journal_article
volume: 10
year: '2006'
...
---
_id: '3007'
abstract:
- lang: eng
text: Root gravitropism describes the orientation of root growth along the gravity
vector and is mediated by differential cell elongation in the root meristem. This
response requires the coordinated, asymmetric distribution of the phytohormone
auxin within the root meristem, and depends on the concerted activities of PIN
proteins and AUX1 - members of the auxin transport pathway. Here, we show that
intracellular trafficking and proteasome activity combine to control PIN2 degradation
during root gravitropism. Following gravi-stimulation, proteasome-dependent variations
in PIN2 localization and degradation at the upper and lower sides of the root
result in asymmetric distribution of PIN2. Ubiquitination of PIN2 occurs in a
proteasome-dependent manner, indicating that the proteasome is involved in the
control of PIN2 turnover. Stabilization of PIN2 affects its abundance and distribution,
and leads to defects in auxin distribution and gravitropic responses. We describe
the effects of auxin on PIN2 localization and protein levels, indicating that
redistribution of auxin during the gravitropic response may be involved in the
regulation of PIN2 protein.
author:
- first_name: Lindy
full_name: Abas, Lindy
last_name: Abas
- first_name: René
full_name: Benjamins, René
last_name: Benjamins
- first_name: Nenad
full_name: Malenica, Nenad
last_name: Malenica
- first_name: Tomasz
full_name: Paciorek, Tomasz
last_name: Paciorek
- first_name: Justyna
full_name: Wiśniewska, Justyna
last_name: Wiśniewska
- first_name: Jeanette
full_name: Moulinier-Anzola, Jeanette C
last_name: Moulinier Anzola
- first_name: Tobias
full_name: Sieberer, Tobias
last_name: Sieberer
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Christian
full_name: Luschnig, Christian
last_name: Luschnig
citation:
ama: Abas L, Benjamins R, Malenica N, et al. Intracellular trafficking and proteolysis
of the Arabidopsis auxin-efflux facilitator PIN2 are involved in root gravitropism.
Nature Cell Biology. 2006;8(3):249-256. doi:10.1038/ncb1369
apa: Abas, L., Benjamins, R., Malenica, N., Paciorek, T., Wiśniewska, J., Moulinier
Anzola, J., … Luschnig, C. (2006). Intracellular trafficking and proteolysis of
the Arabidopsis auxin-efflux facilitator PIN2 are involved in root gravitropism.
Nature Cell Biology. Nature Publishing Group. https://doi.org/10.1038/ncb1369
chicago: Abas, Lindy, René Benjamins, Nenad Malenica, Tomasz Paciorek, Justyna Wiśniewska,
Jeanette Moulinier Anzola, Tobias Sieberer, Jiří Friml, and Christian Luschnig.
“Intracellular Trafficking and Proteolysis of the Arabidopsis Auxin-Efflux Facilitator
PIN2 Are Involved in Root Gravitropism.” Nature Cell Biology. Nature Publishing
Group, 2006. https://doi.org/10.1038/ncb1369.
ieee: L. Abas et al., “Intracellular trafficking and proteolysis of the Arabidopsis
auxin-efflux facilitator PIN2 are involved in root gravitropism,” Nature Cell
Biology, vol. 8, no. 3. Nature Publishing Group, pp. 249–256, 2006.
ista: Abas L, Benjamins R, Malenica N, Paciorek T, Wiśniewska J, Moulinier Anzola
J, Sieberer T, Friml J, Luschnig C. 2006. Intracellular trafficking and proteolysis
of the Arabidopsis auxin-efflux facilitator PIN2 are involved in root gravitropism.
Nature Cell Biology. 8(3), 249–256.
mla: Abas, Lindy, et al. “Intracellular Trafficking and Proteolysis of the Arabidopsis
Auxin-Efflux Facilitator PIN2 Are Involved in Root Gravitropism.” Nature Cell
Biology, vol. 8, no. 3, Nature Publishing Group, 2006, pp. 249–56, doi:10.1038/ncb1369.
short: L. Abas, R. Benjamins, N. Malenica, T. Paciorek, J. Wiśniewska, J. Moulinier
Anzola, T. Sieberer, J. Friml, C. Luschnig, Nature Cell Biology 8 (2006) 249–256.
date_created: 2018-12-11T12:00:50Z
date_published: 2006-03-01T00:00:00Z
date_updated: 2021-01-12T07:40:25Z
day: '01'
doi: 10.1038/ncb1369
extern: 1
intvolume: ' 8'
issue: '3'
month: '03'
page: 249 - 256
publication: Nature Cell Biology
publication_status: published
publisher: Nature Publishing Group
publist_id: '3694'
quality_controlled: 0
status: public
title: Intracellular trafficking and proteolysis of the Arabidopsis auxin-efflux facilitator
PIN2 are involved in root gravitropism
type: journal_article
volume: 8
year: '2006'
...
---
_id: '3008'
abstract:
- lang: eng
text: Plants and some animals have a profound capacity to regenerate organs from
adult tissues. Molecular mechanisms for regeneration have, however, been largely
unexplored. Here we investigate a local regeneration response in Arabidopsis roots.
Laser-induced wounding disrupts the flow of auxin-a cell-fate-instructive plant
hormone-in root tips, and we demonstrate that resulting cell-fate changes require
the PLETHORA, SHORTROOT, and SCARECROW transcription factors. These transcription
factors regulate the expression and polar position of PIN auxin efflux-facilitating
membrane proteins to reconstitute auxin transport in renewed root tips. Thus,
a regeneration mechanism using embryonic root stem-cell patterning factors first
responds to and subsequently stabilizes a new hormone distribution.
author:
- first_name: Jian
full_name: Xu, Jian
last_name: Xu
- first_name: Hugo
full_name: Hofhuis, Hugo
last_name: Hofhuis
- first_name: Renze
full_name: Heidstra, Renze
last_name: Heidstra
- first_name: Michael
full_name: Sauer, Michael
last_name: Sauer
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Ben
full_name: Scheres, Ben
last_name: Scheres
citation:
ama: Xu J, Hofhuis H, Heidstra R, Sauer M, Friml J, Scheres B. A molecular framework
for plant regeneration. Science. 2006;311(5759):385-388. doi:10.1126/science.1121790
apa: Xu, J., Hofhuis, H., Heidstra, R., Sauer, M., Friml, J., & Scheres, B.
(2006). A molecular framework for plant regeneration. Science. American
Association for the Advancement of Science. https://doi.org/10.1126/science.1121790
chicago: Xu, Jian, Hugo Hofhuis, Renze Heidstra, Michael Sauer, Jiří Friml, and
Ben Scheres. “A Molecular Framework for Plant Regeneration.” Science. American
Association for the Advancement of Science, 2006. https://doi.org/10.1126/science.1121790.
ieee: J. Xu, H. Hofhuis, R. Heidstra, M. Sauer, J. Friml, and B. Scheres, “A molecular
framework for plant regeneration,” Science, vol. 311, no. 5759. American
Association for the Advancement of Science, pp. 385–388, 2006.
ista: Xu J, Hofhuis H, Heidstra R, Sauer M, Friml J, Scheres B. 2006. A molecular
framework for plant regeneration. Science. 311(5759), 385–388.
mla: Xu, Jian, et al. “A Molecular Framework for Plant Regeneration.” Science,
vol. 311, no. 5759, American Association for the Advancement of Science, 2006,
pp. 385–88, doi:10.1126/science.1121790.
short: J. Xu, H. Hofhuis, R. Heidstra, M. Sauer, J. Friml, B. Scheres, Science 311
(2006) 385–388.
date_created: 2018-12-11T12:00:50Z
date_published: 2006-01-20T00:00:00Z
date_updated: 2021-01-12T07:40:25Z
day: '20'
doi: 10.1126/science.1121790
extern: 1
intvolume: ' 311'
issue: '5759'
month: '01'
page: 385 - 388
publication: Science
publication_status: published
publisher: American Association for the Advancement of Science
publist_id: '3695'
quality_controlled: 0
status: public
title: A molecular framework for plant regeneration
type: journal_article
volume: 311
year: '2006'
...
---
_id: '3009'
author:
- first_name: Tomasz
full_name: Paciorek, Tomasz
last_name: Paciorek
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Paciorek T, Friml J. Auxin signaling. Journal of Cell Science. 2006;119(7):1199-1202.
doi:10.1242/jcs.02910
apa: Paciorek, T., & Friml, J. (2006). Auxin signaling. Journal of Cell Science.
Company of Biologists. https://doi.org/10.1242/jcs.02910
chicago: Paciorek, Tomasz, and Jiří Friml. “Auxin Signaling.” Journal of Cell
Science. Company of Biologists, 2006. https://doi.org/10.1242/jcs.02910.
ieee: T. Paciorek and J. Friml, “Auxin signaling,” Journal of Cell Science,
vol. 119, no. 7. Company of Biologists, pp. 1199–1202, 2006.
ista: Paciorek T, Friml J. 2006. Auxin signaling. Journal of Cell Science. 119(7),
1199–1202.
mla: Paciorek, Tomasz, and Jiří Friml. “Auxin Signaling.” Journal of Cell Science,
vol. 119, no. 7, Company of Biologists, 2006, pp. 1199–202, doi:10.1242/jcs.02910.
short: T. Paciorek, J. Friml, Journal of Cell Science 119 (2006) 1199–1202.
date_created: 2018-12-11T12:00:50Z
date_published: 2006-01-01T00:00:00Z
date_updated: 2021-01-12T07:40:25Z
day: '01'
doi: 10.1242/jcs.02910
extern: '1'
external_id:
pmid:
- ' 16554435'
intvolume: ' 119'
issue: '7'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pubmed/16554435
month: '01'
oa: 1
oa_version: Published Version
page: 1199 - 1202
pmid: 1
publication: Journal of Cell Science
publication_status: published
publisher: Company of Biologists
publist_id: '3693'
quality_controlled: '1'
status: public
title: Auxin signaling
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 119
year: '2006'
...
---
_id: '3010'
abstract:
- lang: eng
text: The formation of the leaf vascular pattern has fascinated biologists for centuries.
In the early leaf primordium, complex networks of procambial cells emerge from
homogeneous subepidermal tissue. The molecular nature of the underlying positional
information is unknown, but various lines of evidence implicate gradually restricted
transport routes of the plant hormone auxin in defining sites of procambium formation.
Here we show that a crucial member of the AtPIN family of auxin-efflux-associated
proteins, AtPIN1, is expressed prior to pre-procambial and procambial cell fate
markers in domains that become restricted toward sites of procambium formation.
Subcellular AtPIN1 polarity indicates that auxin is directed to distinct "convergence
points" in the epidermis, from where it defines the positions of major veins.
Integrated polarities in all emerging veins indicate auxin drainage toward pre-existing
veins, but veins display divergent polarities as they become connected at both
ends. Auxin application and transport inhibition reveal that convergence point
positioning and AtPIN1 expression domain dynamics are self-organizing, auxin-transport-dependent
processes. We derive a model for self-regulated, reiterative patterning of all
vein orders and postulate at its onset a common epidermal auxin-focusing mechanism
for major-vein positioning and phyllotactic patterning.
author:
- first_name: Enrico
full_name: Scarpella, Enrico
last_name: Scarpella
- first_name: Danielle
full_name: Marcos, Danielle
last_name: Marcos
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Thomas
full_name: Berleth, Thomas
last_name: Berleth
citation:
ama: Scarpella E, Marcos D, Friml J, Berleth T. Control of leaf vascular patterning
by polar auxin transport. Genes and Development. 2006;20(8):1015-1027.
doi:10.1101/gad.1402406
apa: Scarpella, E., Marcos, D., Friml, J., & Berleth, T. (2006). Control of
leaf vascular patterning by polar auxin transport. Genes and Development.
Cold Spring Harbor Laboratory Press. https://doi.org/10.1101/gad.1402406
chicago: Scarpella, Enrico, Danielle Marcos, Jiří Friml, and Thomas Berleth. “Control
of Leaf Vascular Patterning by Polar Auxin Transport.” Genes and Development.
Cold Spring Harbor Laboratory Press, 2006. https://doi.org/10.1101/gad.1402406.
ieee: E. Scarpella, D. Marcos, J. Friml, and T. Berleth, “Control of leaf vascular
patterning by polar auxin transport,” Genes and Development, vol. 20, no.
8. Cold Spring Harbor Laboratory Press, pp. 1015–1027, 2006.
ista: Scarpella E, Marcos D, Friml J, Berleth T. 2006. Control of leaf vascular
patterning by polar auxin transport. Genes and Development. 20(8), 1015–1027.
mla: Scarpella, Enrico, et al. “Control of Leaf Vascular Patterning by Polar Auxin
Transport.” Genes and Development, vol. 20, no. 8, Cold Spring Harbor Laboratory
Press, 2006, pp. 1015–27, doi:10.1101/gad.1402406.
short: E. Scarpella, D. Marcos, J. Friml, T. Berleth, Genes and Development 20 (2006)
1015–1027.
date_created: 2018-12-11T12:00:51Z
date_published: 2006-04-15T00:00:00Z
date_updated: 2021-01-12T07:40:26Z
day: '15'
doi: 10.1101/gad.1402406
extern: 1
intvolume: ' 20'
issue: '8'
month: '04'
page: 1015 - 1027
publication: Genes and Development
publication_status: published
publisher: Cold Spring Harbor Laboratory Press
publist_id: '3692'
quality_controlled: 0
status: public
title: Control of leaf vascular patterning by polar auxin transport
type: journal_article
volume: 20
year: '2006'
...
---
_id: '3011'
abstract:
- lang: eng
text: Polar flow of the phytohormone auxin requires plasma membrane‐associated PIN
proteins and underlies multiple developmental processes in plants. Here we address
the importance of the polarity of subcellular PIN localization for the directionality
of auxin transport in Arabidopsis thaliana. Expression of different PINs in the
root epidermis revealed the importance of PIN polar positions for directional
auxin flow and root gravitropic growth. Interfering with sequence-embedded polarity
signals directly demonstrates that PIN polarity is a primary factor in determining
the direction of auxin flow in meristematic tissues. This finding provides a crucial
piece in the puzzle of how auxin flow can be redirected via rapid changes in PIN
polarity.
author:
- first_name: Justyna
full_name: Wiśniewska, Justyna
last_name: Wiśniewska
- first_name: Jian
full_name: Xu, Jian
last_name: Xu
- first_name: Daniela
full_name: Seifertová, Daniela
last_name: Seifertová
- first_name: Philip
full_name: Brewer, Philip B
last_name: Brewer
- first_name: Kamil
full_name: Růžička, Kamil
last_name: Růžička
- first_name: Ikram
full_name: Blilou, Ikram
last_name: Blilou
- first_name: David
full_name: Rouquié, David
last_name: Rouquié
- first_name: Eva
full_name: Eva Benková
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Ben
full_name: Scheres, Ben
last_name: Scheres
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Wiśniewska J, Xu J, Seifertová D, et al. Polar PIN localization directs auxin
flow in plants. Science. 2006;312(5775). doi:10.1126/science.1121356
apa: Wiśniewska, J., Xu, J., Seifertová, D., Brewer, P., Růžička, K., Blilou, I.,
… Friml, J. (2006). Polar PIN localization directs auxin flow in plants. Science.
American Association for the Advancement of Science. https://doi.org/10.1126/science.1121356
chicago: Wiśniewska, Justyna, Jian Xu, Daniela Seifertová, Philip Brewer, Kamil
Růžička, Ikram Blilou, David Rouquié, Eva Benková, Ben Scheres, and Jiří Friml.
“Polar PIN Localization Directs Auxin Flow in Plants.” Science. American
Association for the Advancement of Science, 2006. https://doi.org/10.1126/science.1121356.
ieee: J. Wiśniewska et al., “Polar PIN localization directs auxin flow in
plants,” Science, vol. 312, no. 5775. American Association for the Advancement
of Science, 2006.
ista: Wiśniewska J, Xu J, Seifertová D, Brewer P, Růžička K, Blilou I, Rouquié D,
Benková E, Scheres B, Friml J. 2006. Polar PIN localization directs auxin flow
in plants. Science. 312(5775).
mla: Wiśniewska, Justyna, et al. “Polar PIN Localization Directs Auxin Flow in Plants.”
Science, vol. 312, no. 5775, American Association for the Advancement of
Science, 2006, doi:10.1126/science.1121356.
short: J. Wiśniewska, J. Xu, D. Seifertová, P. Brewer, K. Růžička, I. Blilou, D.
Rouquié, E. Benková, B. Scheres, J. Friml, Science 312 (2006).
date_created: 2018-12-11T12:00:51Z
date_published: 2006-05-12T00:00:00Z
date_updated: 2021-01-12T07:40:27Z
day: '12'
doi: 10.1126/science.1121356
extern: 1
intvolume: ' 312'
issue: '5775'
month: '05'
publication: Science
publication_status: published
publisher: American Association for the Advancement of Science
publist_id: '3691'
quality_controlled: 0
status: public
title: Polar PIN localization directs auxin flow in plants
type: journal_article
volume: 312
year: '2006'
...
---
_id: '3012'
abstract:
- lang: eng
text: Intercellular flow of the phytohormone auxin underpins multiple developmental
processes in plants. Plant-specific pin-formed (PIN) proteins and several phosphoglycoprotein
(PGP) transporters are crucial factors in auxin transport-related development,
yet the molecular function of PINs remains unknown. Here, we show that PINs mediate
auxin efflux from mammalian and yeast cells without needing additional plant-specific
factors. Conditional gain-of-function alleles and quantitative measurements of
auxin accumulation in Arabidopsis and tobacco cultured cells revealed that the
action of PINs in auxin efflux is distinct from PGP, rate-limiting, specific to
auxins, and sensitive to auxin transport inhibitors. This suggests a direct involvement
of PINs in catalyzing cellular auxin efflux.
author:
- first_name: Jan
full_name: Petrášek, Jan
last_name: Petrášek
- first_name: Jozef
full_name: Mravec, Jozef
last_name: Mravec
- first_name: Rodolphe
full_name: Bouchard, Rodolphe
last_name: Bouchard
- first_name: Joshua
full_name: Blakeslee, Joshua
last_name: Blakeslee
- first_name: Melinda F
full_name: Melinda Abas
id: 3CFB3B1C-F248-11E8-B48F-1D18A9856A87
last_name: Abas
- first_name: Daniela
full_name: Seifertová, Daniela
last_name: Seifertová
- first_name: Justyna
full_name: Wiśniewska, Justyna
last_name: Wiśniewska
- first_name: Zerihun
full_name: Tadele, Zerihun
last_name: Tadele
- first_name: Martin
full_name: Kubeš, Martin
last_name: Kubeš
- first_name: Milada
full_name: Čovanová, Milada
last_name: Čovanová
- first_name: Pankaj
full_name: Dhonukshe, Pankaj
last_name: Dhonukshe
- first_name: Petr
full_name: Skůpa, Petr
last_name: Skůpa
- first_name: Eva
full_name: Eva Benková
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Lucie
full_name: Perry, Lucie
last_name: Perry
- first_name: Pavel
full_name: Křeček, Pavel
last_name: Křeček
- first_name: Ok
full_name: Lee, Ok Ran
last_name: Lee
- first_name: Gerald
full_name: Fink, Gerald R
last_name: Fink
- first_name: Markus
full_name: Geisler, Markus
last_name: Geisler
- first_name: Angus
full_name: Murphy, Angus S
last_name: Murphy
- first_name: Christian
full_name: Luschnig, Christian
last_name: Luschnig
- first_name: Eva
full_name: Zažímalová, Eva
last_name: Zažímalová
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Petrášek J, Mravec J, Bouchard R, et al. PIN proteins perform a rate-limiting
function in cellular auxin efflux. Science. 2006;312(5775):914-918. doi:10.1126/science.1123542
apa: Petrášek, J., Mravec, J., Bouchard, R., Blakeslee, J., Abas, M. F., Seifertová,
D., … Friml, J. (2006). PIN proteins perform a rate-limiting function in cellular
auxin efflux. Science. American Association for the Advancement of Science.
https://doi.org/10.1126/science.1123542
chicago: Petrášek, Jan, Jozef Mravec, Rodolphe Bouchard, Joshua Blakeslee, Melinda
F Abas, Daniela Seifertová, Justyna Wiśniewska, et al. “PIN Proteins Perform a
Rate-Limiting Function in Cellular Auxin Efflux.” Science. American Association
for the Advancement of Science, 2006. https://doi.org/10.1126/science.1123542.
ieee: J. Petrášek et al., “PIN proteins perform a rate-limiting function
in cellular auxin efflux,” Science, vol. 312, no. 5775. American Association
for the Advancement of Science, pp. 914–918, 2006.
ista: Petrášek J, Mravec J, Bouchard R, Blakeslee J, Abas MF, Seifertová D, Wiśniewska
J, Tadele Z, Kubeš M, Čovanová M, Dhonukshe P, Skůpa P, Benková E, Perry L, Křeček
P, Lee O, Fink G, Geisler M, Murphy A, Luschnig C, Zažímalová E, Friml J. 2006.
PIN proteins perform a rate-limiting function in cellular auxin efflux. Science.
312(5775), 914–918.
mla: Petrášek, Jan, et al. “PIN Proteins Perform a Rate-Limiting Function in Cellular
Auxin Efflux.” Science, vol. 312, no. 5775, American Association for the
Advancement of Science, 2006, pp. 914–18, doi:10.1126/science.1123542.
short: J. Petrášek, J. Mravec, R. Bouchard, J. Blakeslee, M.F. Abas, D. Seifertová,
J. Wiśniewska, Z. Tadele, M. Kubeš, M. Čovanová, P. Dhonukshe, P. Skůpa, E. Benková,
L. Perry, P. Křeček, O. Lee, G. Fink, M. Geisler, A. Murphy, C. Luschnig, E. Zažímalová,
J. Friml, Science 312 (2006) 914–918.
date_created: 2018-12-11T12:00:51Z
date_published: 2006-05-12T00:00:00Z
date_updated: 2021-01-12T07:40:27Z
day: '12'
doi: 10.1126/science.1123542
extern: 1
intvolume: ' 312'
issue: '5775'
month: '05'
page: 914 - 918
publication: Science
publication_status: published
publisher: American Association for the Advancement of Science
publist_id: '3690'
quality_controlled: 0
status: public
title: PIN proteins perform a rate-limiting function in cellular auxin efflux
type: journal_article
volume: 312
year: '2006'
...
---
_id: '3013'
abstract:
- lang: eng
text: 'There is a growing demand for methods that allow rapid and reliable in situ
localization of proteins in plant cells. The immunocytochemistry protocol presented
here can be used routinely to observe protein localization patterns in tissue
sections of various plant species. This protocol is especially suitable for plant
species with more-complex tissue architecture (such as maize, Zea mays), which
makes it difficult to use an easier whole-mount procedure for protein localization.
To facilitate the antibody-antigen reaction, it is necessary to include a wax-embedding
and tissue-sectioning step. The protocol consists of the following procedures:
chemical fixation of tissue, dehydration, wax embedding, sectioning, dewaxing,
rehydration, blocking and antibody incubation. The detailed protocol, recommended
controls and troubleshooting are presented here, along with examples of applications.'
author:
- first_name: Tomasz
full_name: Paciorek, Tomasz
last_name: Paciorek
- first_name: Michael
full_name: Sauer, Michael
last_name: Sauer
- first_name: Jozef
full_name: Balla, Jozef
last_name: Balla
- first_name: Justyna
full_name: Wiśniewska, Justyna
last_name: Wiśniewska
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Paciorek T, Sauer M, Balla J, Wiśniewska J, Friml J. Immunocytochemical technique
for protein localization in sections of plant tissues. Nature Protocols.
2006;1(1):104-107. doi:10.1038/nprot.2006.16
apa: Paciorek, T., Sauer, M., Balla, J., Wiśniewska, J., & Friml, J. (2006).
Immunocytochemical technique for protein localization in sections of plant tissues.
Nature Protocols. Nature Publishing Group. https://doi.org/10.1038/nprot.2006.16
chicago: Paciorek, Tomasz, Michael Sauer, Jozef Balla, Justyna Wiśniewska, and Jiří
Friml. “Immunocytochemical Technique for Protein Localization in Sections of Plant
Tissues.” Nature Protocols. Nature Publishing Group, 2006. https://doi.org/10.1038/nprot.2006.16.
ieee: T. Paciorek, M. Sauer, J. Balla, J. Wiśniewska, and J. Friml, “Immunocytochemical
technique for protein localization in sections of plant tissues,” Nature Protocols,
vol. 1, no. 1. Nature Publishing Group, pp. 104–107, 2006.
ista: Paciorek T, Sauer M, Balla J, Wiśniewska J, Friml J. 2006. Immunocytochemical
technique for protein localization in sections of plant tissues. Nature Protocols.
1(1), 104–107.
mla: Paciorek, Tomasz, et al. “Immunocytochemical Technique for Protein Localization
in Sections of Plant Tissues.” Nature Protocols, vol. 1, no. 1, Nature
Publishing Group, 2006, pp. 104–07, doi:10.1038/nprot.2006.16.
short: T. Paciorek, M. Sauer, J. Balla, J. Wiśniewska, J. Friml, Nature Protocols
1 (2006) 104–107.
date_created: 2018-12-11T12:00:52Z
date_published: 2006-06-01T00:00:00Z
date_updated: 2021-01-12T07:40:27Z
day: '01'
doi: 10.1038/nprot.2006.16
extern: 1
intvolume: ' 1'
issue: '1'
month: '06'
page: 104 - 107
publication: Nature Protocols
publication_status: published
publisher: Nature Publishing Group
publist_id: '3689'
quality_controlled: 0
status: public
title: Immunocytochemical technique for protein localization in sections of plant
tissues
type: journal_article
volume: 1
year: '2006'
...