---
_id: '3067'
abstract:
- lang: eng
text: Remarkable progress in various techniques of in vivo fluorescence microscopy
has brought an urgent need for reliable markers for tracking cellular structures
and processes. The goal of this manuscript is to describe unexplored effects of
the FM (Fei Mao) styryl dyes, which are widely used probes that label processes
of endocytosis and vesicle trafficking in eukaryotic cells. Although there are
few reports on the effect of styryl dyes on membrane fluidity and the activity
of mammalian receptors, FM dyes have been considered as reliable tools for tracking
of plant endocytosis. Using plasma membrane-localized transporters for the plant
hormone auxin in tobacco BY-2 and Arabidopsis thaliana cell suspensions, we show
that routinely used concentrations of FM 4-64 and FM 5-95 trigger transient re-localization
of these proteins, and FM 1-43 affects their activity. The active process of re-localization
is blocked neither by inhibitors of endocytosis nor by cytoskeletal drugs. It
does not occur in A. thaliana roots and depends on the degree of hydrophobicity
(lipophilicity) of a particular FM dye. Our results emphasize the need for circumspection
during in vivo studies of membrane proteins performed using simultaneous labelling
with FM dyes.
author:
- first_name: Adriana
full_name: Jelínková, Adriana
last_name: Jelínková
- first_name: Kateřina
full_name: Malínská, Kateřina
last_name: Malínská
- first_name: Sibu
full_name: Sibu Simon
id: 4542EF9A-F248-11E8-B48F-1D18A9856A87
last_name: Simon
orcid: 0000-0002-1998-6741
- first_name: Jürgen
full_name: Kleine-Vehn, Jürgen
last_name: Kleine Vehn
- first_name: Markéta
full_name: Pařezová, Markéta
last_name: Pařezová
- first_name: Přemysl
full_name: Pejchar, Přemysl
last_name: Pejchar
- first_name: Martin
full_name: Kubeš, Martin
last_name: Kubeš
- first_name: Jan
full_name: Martinec, Jan
last_name: Martinec
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Eva
full_name: Zažímalová, Eva
last_name: Zažímalová
- first_name: Jan
full_name: Petrášek, Jan
last_name: Petrášek
citation:
ama: 'Jelínková A, Malínská K, Simon S, et al. Probing plant membranes with FM dyes:
Tracking dragging or blocking? Plant Journal. 2010;61(5):883-892. doi:10.1111/j.1365-313X.2009.04102.x'
apa: 'Jelínková, A., Malínská, K., Simon, S., Kleine Vehn, J., Pařezová, M., Pejchar,
P., … Petrášek, J. (2010). Probing plant membranes with FM dyes: Tracking dragging
or blocking? Plant Journal. Wiley-Blackwell. https://doi.org/10.1111/j.1365-313X.2009.04102.x'
chicago: 'Jelínková, Adriana, Kateřina Malínská, Sibu Simon, Jürgen Kleine Vehn,
Markéta Pařezová, Přemysl Pejchar, Martin Kubeš, et al. “Probing Plant Membranes
with FM Dyes: Tracking Dragging or Blocking?” Plant Journal. Wiley-Blackwell,
2010. https://doi.org/10.1111/j.1365-313X.2009.04102.x.'
ieee: 'A. Jelínková et al., “Probing plant membranes with FM dyes: Tracking
dragging or blocking?,” Plant Journal, vol. 61, no. 5. Wiley-Blackwell,
pp. 883–892, 2010.'
ista: 'Jelínková A, Malínská K, Simon S, Kleine Vehn J, Pařezová M, Pejchar P, Kubeš
M, Martinec J, Friml J, Zažímalová E, Petrášek J. 2010. Probing plant membranes
with FM dyes: Tracking dragging or blocking? Plant Journal. 61(5), 883–892.'
mla: 'Jelínková, Adriana, et al. “Probing Plant Membranes with FM Dyes: Tracking
Dragging or Blocking?” Plant Journal, vol. 61, no. 5, Wiley-Blackwell,
2010, pp. 883–92, doi:10.1111/j.1365-313X.2009.04102.x.'
short: A. Jelínková, K. Malínská, S. Simon, J. Kleine Vehn, M. Pařezová, P. Pejchar,
M. Kubeš, J. Martinec, J. Friml, E. Zažímalová, J. Petrášek, Plant Journal 61
(2010) 883–892.
date_created: 2018-12-11T12:01:10Z
date_published: 2010-03-01T00:00:00Z
date_updated: 2021-01-12T07:40:49Z
day: '01'
doi: 10.1111/j.1365-313X.2009.04102.x
extern: 1
intvolume: ' 61'
issue: '5'
month: '03'
page: 883 - 892
publication: Plant Journal
publication_status: published
publisher: Wiley-Blackwell
publist_id: '3635'
quality_controlled: 0
status: public
title: 'Probing plant membranes with FM dyes: Tracking dragging or blocking?'
type: journal_article
volume: 61
year: '2010'
...
---
_id: '3068'
abstract:
- lang: eng
text: Differential distribution of the plant hormone auxin within tissues mediates
a variety of developmental processes. Cellular auxin levels are determined by
metabolic processes including synthesis, degradation, and (de)conjugation, as
well as by auxin transport across the plasma membrane. Whereas transport of free
auxins such as naturally occurring indole-3-acetic acid (IAA) is well characterized,
little is known about the transport of auxin precursors and metabolites. Here,
we identify amutation in the ABCG37 gene of Arabidopsis that causes the polar
auxin transport inhibitor sensitive1 (pis1) phenotype manifested by hypersensitivity
to auxinic compounds. ABCG37 encodes the pleiotropic drug resistance transporter
that transports a range of synthetic auxinic compounds as well as the endogenous
auxin precursor indole-3-butyric acid (IBA), but not free IAA. ABCG37 and its
homolog ABCG36 act redundantly at outermost root plasma membranes and,unlike established
IAA transporters from the PIN and ABCB families, transport IBA out of the cells.
Our findings explore possible novel modes of regulating auxin homeostasis and
plant development by means of directional transport of the auxin precursor IBA
and presumably also other auxin metabolites.
author:
- first_name: Kamil
full_name: Růžička, Kamil
last_name: Růžička
- first_name: Lucia
full_name: Strader, Lucia C
last_name: Strader
- first_name: Aurélien
full_name: Bailly, Aurélien
last_name: Bailly
- first_name: Haibing
full_name: Yang, Haibing
last_name: Yang
- first_name: Joshua
full_name: Blakeslee, Joshua
last_name: Blakeslee
- first_name: Łukasz
full_name: Łangowski, Łukasz
last_name: Łangowski
- first_name: Eliška
full_name: Nejedlá, Eliška
last_name: Nejedlá
- first_name: Hironori
full_name: Fujita, Hironori
last_name: Fujita
- first_name: Hironori
full_name: Itoh, Hironori
last_name: Itoh
- first_name: Kunihiko
full_name: Syōno, Kunihiko
last_name: Syōno
- first_name: Jan
full_name: Hejátko, Jan
last_name: Hejátko
- first_name: William
full_name: Gray, William M
last_name: Gray
- first_name: Enrico
full_name: Martinoia, Enrico
last_name: Martinoia
- first_name: Markus
full_name: Geisler, Markus
last_name: Geisler
- first_name: Bonnie
full_name: Bartel, Bonnie
last_name: Bartel
- first_name: Angus
full_name: Murphy, Angus S
last_name: Murphy
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Růžička K, Strader L, Bailly A, et al. Arabidopsis PIS1 encodes the ABCG37
transporter of auxinic compounds including the auxin precursor indole 3 butyric
acid. PNAS. 2010;107(23):10749-10753. doi:10.1073/pnas.1005878107
apa: Růžička, K., Strader, L., Bailly, A., Yang, H., Blakeslee, J., Łangowski, Ł.,
… Friml, J. (2010). Arabidopsis PIS1 encodes the ABCG37 transporter of auxinic
compounds including the auxin precursor indole 3 butyric acid. PNAS. National
Academy of Sciences. https://doi.org/10.1073/pnas.1005878107
chicago: Růžička, Kamil, Lucia Strader, Aurélien Bailly, Haibing Yang, Joshua Blakeslee,
Łukasz Łangowski, Eliška Nejedlá, et al. “Arabidopsis PIS1 Encodes the ABCG37
Transporter of Auxinic Compounds Including the Auxin Precursor Indole 3 Butyric
Acid.” PNAS. National Academy of Sciences, 2010. https://doi.org/10.1073/pnas.1005878107.
ieee: K. Růžička et al., “Arabidopsis PIS1 encodes the ABCG37 transporter
of auxinic compounds including the auxin precursor indole 3 butyric acid,” PNAS,
vol. 107, no. 23. National Academy of Sciences, pp. 10749–10753, 2010.
ista: Růžička K, Strader L, Bailly A, Yang H, Blakeslee J, Łangowski Ł, Nejedlá
E, Fujita H, Itoh H, Syōno K, Hejátko J, Gray W, Martinoia E, Geisler M, Bartel
B, Murphy A, Friml J. 2010. Arabidopsis PIS1 encodes the ABCG37 transporter of
auxinic compounds including the auxin precursor indole 3 butyric acid. PNAS. 107(23),
10749–10753.
mla: Růžička, Kamil, et al. “Arabidopsis PIS1 Encodes the ABCG37 Transporter of
Auxinic Compounds Including the Auxin Precursor Indole 3 Butyric Acid.” PNAS,
vol. 107, no. 23, National Academy of Sciences, 2010, pp. 10749–53, doi:10.1073/pnas.1005878107.
short: K. Růžička, L. Strader, A. Bailly, H. Yang, J. Blakeslee, Ł. Łangowski, E.
Nejedlá, H. Fujita, H. Itoh, K. Syōno, J. Hejátko, W. Gray, E. Martinoia, M. Geisler,
B. Bartel, A. Murphy, J. Friml, PNAS 107 (2010) 10749–10753.
date_created: 2018-12-11T12:01:11Z
date_published: 2010-06-08T00:00:00Z
date_updated: 2021-01-12T07:40:49Z
day: '08'
doi: 10.1073/pnas.1005878107
extern: 1
intvolume: ' 107'
issue: '23'
month: '06'
page: 10749 - 10753
publication: PNAS
publication_status: published
publisher: National Academy of Sciences
publist_id: '3633'
quality_controlled: 0
status: public
title: Arabidopsis PIS1 encodes the ABCG37 transporter of auxinic compounds including
the auxin precursor indole 3 butyric acid
type: journal_article
volume: 107
year: '2010'
...
---
_id: '3069'
abstract:
- lang: eng
text: The stem cell niche in the root meristem is critical for the development of
the plant root system. The plant hormone auxin acts as a versatile trigger in
many developmental processes, including the regulation of root growth, but its
role in the control of the stem cell activity remains largely unclear. Here we
show that local auxin levels, determined by biosynthesis and intercellular transport,
mediate maintenance or differentiation of distal stem cells in the Arabidopsis
thaliana roots. Genetic analysis shows that auxin acts upstream of the major regulators
of the stem cell activity, the homeodomain transcription factor WOX5, and the
AP-2 transcription factor PLETHORA. Auxin signaling for differentiation of distal
stem cells requires the transcriptional repressor IAA17/AXR3 as well as the ARF10
and ARF16 auxin response factors. ARF10 and ARF16 activities repress the WOX5
transcription and restrict it to the quiescent center, where WOX5, in turn, is
needed for the activity of PLETHORA. Our investigations reveal that long-distance
auxin signals act upstream of the short-range network of transcriptional factors
to mediate the differentiation of distal stem cells in roots.
author:
- first_name: Zhaojun
full_name: Ding, Zhaojun
last_name: Ding
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Ding Z, Friml J. Auxin regulates distal stem cell differentiation in Arabidopsis
roots. PNAS. 2010;107(26):12046-12051. doi:10.1073/pnas.1000672107
apa: Ding, Z., & Friml, J. (2010). Auxin regulates distal stem cell differentiation
in Arabidopsis roots. PNAS. National Academy of Sciences. https://doi.org/10.1073/pnas.1000672107
chicago: Ding, Zhaojun, and Jiří Friml. “Auxin Regulates Distal Stem Cell Differentiation
in Arabidopsis Roots.” PNAS. National Academy of Sciences, 2010. https://doi.org/10.1073/pnas.1000672107.
ieee: Z. Ding and J. Friml, “Auxin regulates distal stem cell differentiation in
Arabidopsis roots,” PNAS, vol. 107, no. 26. National Academy of Sciences,
pp. 12046–12051, 2010.
ista: Ding Z, Friml J. 2010. Auxin regulates distal stem cell differentiation in
Arabidopsis roots. PNAS. 107(26), 12046–12051.
mla: Ding, Zhaojun, and Jiří Friml. “Auxin Regulates Distal Stem Cell Differentiation
in Arabidopsis Roots.” PNAS, vol. 107, no. 26, National Academy of Sciences,
2010, pp. 12046–51, doi:10.1073/pnas.1000672107.
short: Z. Ding, J. Friml, PNAS 107 (2010) 12046–12051.
date_created: 2018-12-11T12:01:11Z
date_published: 2010-06-29T00:00:00Z
date_updated: 2021-01-12T07:40:50Z
day: '29'
doi: 10.1073/pnas.1000672107
extern: 1
intvolume: ' 107'
issue: '26'
month: '06'
page: 12046 - 12051
publication: PNAS
publication_status: published
publisher: National Academy of Sciences
publist_id: '3632'
quality_controlled: 0
status: public
title: Auxin regulates distal stem cell differentiation in Arabidopsis roots
type: journal_article
volume: 107
year: '2010'
...
---
_id: '3070'
abstract:
- lang: eng
text: Division of the Arabidopsis zygote defines two fundamentally different developmental
domains, the proembryo and suspensor. The resulting boundary separates domain-specific
gene expression, and a signal originating from the proembryo instructs the suspensor
to generate the root stem cell niche. While root induction is known to require
the phytohormone auxin and the Auxin Response Factor MONOPTEROS, it has remained
largely elusive how the two domains involved in this process are initially specified.
Here, we show that the GATA factor HANABA TARANU (HAN) is required to position
the inductive proembryo boundary. Mutations in HAN cause a coordinated apical
shift of gene expression patterns, revealing that HAN regulates transcription
in the basal proembryo. Key auxin transporters are affected as early as the 8
cell stage, resulting in apical redistribution of auxin. Remarkably, han embryos
eventually organize a root independent of MONOPTEROS and the suspensor around
a new boundary marked by the auxin maximum.
author:
- first_name: Tal
full_name: Nawy, Tal
last_name: Nawy
- first_name: Martin
full_name: Bayer, Martin
last_name: Bayer
- first_name: Jozef
full_name: Mravec, Jozef
last_name: Mravec
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Kenneth
full_name: Birnbaum, Kenneth D
last_name: Birnbaum
- first_name: Wolfgang
full_name: Lukowitz, Wolfgang
last_name: Lukowitz
citation:
ama: Nawy T, Bayer M, Mravec J, Friml J, Birnbaum K, Lukowitz W. The GATA factor
HANABA TARANU is required to position the proembryo boundary in the early Arabidopsis
embryo. Developmental Cell. 2010;19(1):103-113. doi:10.1016/j.devcel.2010.06.004
apa: Nawy, T., Bayer, M., Mravec, J., Friml, J., Birnbaum, K., & Lukowitz, W.
(2010). The GATA factor HANABA TARANU is required to position the proembryo boundary
in the early Arabidopsis embryo. Developmental Cell. Cell Press. https://doi.org/10.1016/j.devcel.2010.06.004
chicago: Nawy, Tal, Martin Bayer, Jozef Mravec, Jiří Friml, Kenneth Birnbaum, and
Wolfgang Lukowitz. “The GATA Factor HANABA TARANU Is Required to Position the
Proembryo Boundary in the Early Arabidopsis Embryo.” Developmental Cell.
Cell Press, 2010. https://doi.org/10.1016/j.devcel.2010.06.004.
ieee: T. Nawy, M. Bayer, J. Mravec, J. Friml, K. Birnbaum, and W. Lukowitz, “The
GATA factor HANABA TARANU is required to position the proembryo boundary in the
early Arabidopsis embryo,” Developmental Cell, vol. 19, no. 1. Cell Press,
pp. 103–113, 2010.
ista: Nawy T, Bayer M, Mravec J, Friml J, Birnbaum K, Lukowitz W. 2010. The GATA
factor HANABA TARANU is required to position the proembryo boundary in the early
Arabidopsis embryo. Developmental Cell. 19(1), 103–113.
mla: Nawy, Tal, et al. “The GATA Factor HANABA TARANU Is Required to Position the
Proembryo Boundary in the Early Arabidopsis Embryo.” Developmental Cell,
vol. 19, no. 1, Cell Press, 2010, pp. 103–13, doi:10.1016/j.devcel.2010.06.004.
short: T. Nawy, M. Bayer, J. Mravec, J. Friml, K. Birnbaum, W. Lukowitz, Developmental
Cell 19 (2010) 103–113.
date_created: 2018-12-11T12:01:11Z
date_published: 2010-07-01T00:00:00Z
date_updated: 2021-01-12T07:40:50Z
day: '01'
doi: 10.1016/j.devcel.2010.06.004
extern: 1
intvolume: ' 19'
issue: '1'
month: '07'
page: 103 - 113
publication: Developmental Cell
publication_status: published
publisher: Cell Press
publist_id: '3631'
quality_controlled: 0
status: public
title: The GATA factor HANABA TARANU is required to position the proembryo boundary
in the early Arabidopsis embryo
type: journal_article
volume: 19
year: '2010'
...
---
_id: '3071'
abstract:
- lang: eng
text: Plant vacuoles are essential multifunctional organelles largely distinct from
similar organelles in other eukaryotes. Embryo protein storage vacuoles and the
lytic vacuoles that perform a general degradation function are the best characterized,
but little is known about the biogenesis and transition between these vacuolar
types. Here, we designed a fluorescent marker- based forward genetic screen in
Arabidopsis thaliana and identified a protein affected trafficking2 (pat2) mutant,
whose lytic vacuoles display altered morphology and accumulation of proteins.
Unlike other mutants affecting the vacuole, pat2 is specifically defective in
the biogenesis, identity, and function of lytic vacuoles but shows normal sorting
of proteins to storage vacuoles. PAT2 encodes a putative β-subunit of adaptor
protein complex 3 (AP-3) that can partially complement the corresponding yeast
mutant. Manipulations of the putative AP-3 β adaptin functions suggest a plant-specific
role for the evolutionarily conserved AP-3 β in mediating lytic vacuole performance
and transition of storage into the lytic vacuoles independently of the main prevacuolar
compartment-based trafficking route.
author:
- first_name: Elena
full_name: Feraru, Elena
last_name: Feraru
- first_name: Tomasz
full_name: Paciorek, Tomasz
last_name: Paciorek
- first_name: Mugurel
full_name: Feraru, Mugurel I
last_name: Feraru
- first_name: Marta
full_name: Zwiewka, Marta
last_name: Zwiewka
- first_name: Ruth
full_name: De Groodt, Ruth
last_name: De Groodt
- first_name: Riet
full_name: De Rycke, Riet M
last_name: De Rycke
- first_name: Jürgen
full_name: Kleine-Vehn, Jürgen
last_name: Kleine Vehn
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Feraru E, Paciorek T, Feraru M, et al. The AP 3 β adaptin mediates the biogenesis
and function of lytic vacuoles in Arabidopsis. Plant Cell. 2010;22(8):2812-2824.
doi:10.1105/tpc.110.075424
apa: Feraru, E., Paciorek, T., Feraru, M., Zwiewka, M., De Groodt, R., De Rycke,
R., … Friml, J. (2010). The AP 3 β adaptin mediates the biogenesis and function
of lytic vacuoles in Arabidopsis. Plant Cell. American Society of Plant
Biologists. https://doi.org/10.1105/tpc.110.075424
chicago: Feraru, Elena, Tomasz Paciorek, Mugurel Feraru, Marta Zwiewka, Ruth De
Groodt, Riet De Rycke, Jürgen Kleine Vehn, and Jiří Friml. “The AP 3 β Adaptin
Mediates the Biogenesis and Function of Lytic Vacuoles in Arabidopsis.” Plant
Cell. American Society of Plant Biologists, 2010. https://doi.org/10.1105/tpc.110.075424.
ieee: E. Feraru et al., “The AP 3 β adaptin mediates the biogenesis and function
of lytic vacuoles in Arabidopsis,” Plant Cell, vol. 22, no. 8. American
Society of Plant Biologists, pp. 2812–2824, 2010.
ista: Feraru E, Paciorek T, Feraru M, Zwiewka M, De Groodt R, De Rycke R, Kleine
Vehn J, Friml J. 2010. The AP 3 β adaptin mediates the biogenesis and function
of lytic vacuoles in Arabidopsis. Plant Cell. 22(8), 2812–2824.
mla: Feraru, Elena, et al. “The AP 3 β Adaptin Mediates the Biogenesis and Function
of Lytic Vacuoles in Arabidopsis.” Plant Cell, vol. 22, no. 8, American
Society of Plant Biologists, 2010, pp. 2812–24, doi:10.1105/tpc.110.075424.
short: E. Feraru, T. Paciorek, M. Feraru, M. Zwiewka, R. De Groodt, R. De Rycke,
J. Kleine Vehn, J. Friml, Plant Cell 22 (2010) 2812–2824.
date_created: 2018-12-11T12:01:12Z
date_published: 2010-08-01T00:00:00Z
date_updated: 2021-01-12T07:40:51Z
day: '01'
doi: 10.1105/tpc.110.075424
extern: 1
intvolume: ' 22'
issue: '8'
month: '08'
page: 2812 - 2824
publication: Plant Cell
publication_status: published
publisher: American Society of Plant Biologists
publist_id: '3630'
quality_controlled: 0
status: public
title: The AP 3 β adaptin mediates the biogenesis and function of lytic vacuoles in
Arabidopsis
type: journal_article
volume: 22
year: '2010'
...
---
_id: '3072'
abstract:
- lang: eng
text: Development of plants and their adaptive capacity towards ever‐changing environmental
conditions largely depend on the spatial distribution of the plant hormone auxin.
At the cellular level, various internal and external signals are translated into
specific changes in the polar, subcellular localization of auxin transporters
from the PIN family thereby directing and redirecting the intercellular fluxes
of auxin. The current model of polar targeting of PIN proteins towards different
plasma membrane domains encompasses apolar secretion of newly synthesized PINs
followed by endocytosis and recycling back to the plasma membrane in a polarized
manner. In this review, we follow the subcellular march of the PINs and highlight
the cellular and molecular mechanisms behind polar foraging and subcellular trafficking
pathways. Also, the entry points for different signals and regulations including
by auxin itself will be discussed within the context of morphological and developmental
consequences of polar targeting and subcellular trafficking.
author:
- first_name: Wim
full_name: Grunewald, Wim
last_name: Grunewald
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: 'Grunewald W, Friml J. The march of the PINs: Developmental plasticity by dynamic
polar targeting in plant cells. EMBO Journal. 2010;29(16):2700-2714. doi:10.1038/emboj.2010.181'
apa: 'Grunewald, W., & Friml, J. (2010). The march of the PINs: Developmental
plasticity by dynamic polar targeting in plant cells. EMBO Journal. Wiley-Blackwell.
https://doi.org/10.1038/emboj.2010.181'
chicago: 'Grunewald, Wim, and Jiří Friml. “The March of the PINs: Developmental
Plasticity by Dynamic Polar Targeting in Plant Cells.” EMBO Journal. Wiley-Blackwell,
2010. https://doi.org/10.1038/emboj.2010.181.'
ieee: 'W. Grunewald and J. Friml, “The march of the PINs: Developmental plasticity
by dynamic polar targeting in plant cells,” EMBO Journal, vol. 29, no.
16. Wiley-Blackwell, pp. 2700–2714, 2010.'
ista: 'Grunewald W, Friml J. 2010. The march of the PINs: Developmental plasticity
by dynamic polar targeting in plant cells. EMBO Journal. 29(16), 2700–2714.'
mla: 'Grunewald, Wim, and Jiří Friml. “The March of the PINs: Developmental Plasticity
by Dynamic Polar Targeting in Plant Cells.” EMBO Journal, vol. 29, no.
16, Wiley-Blackwell, 2010, pp. 2700–14, doi:10.1038/emboj.2010.181.'
short: W. Grunewald, J. Friml, EMBO Journal 29 (2010) 2700–2714.
date_created: 2018-12-11T12:01:12Z
date_published: 2010-08-18T00:00:00Z
date_updated: 2021-01-12T07:40:51Z
day: '18'
doi: 10.1038/emboj.2010.181
extern: '1'
external_id:
pmid:
- '20717140'
intvolume: ' 29'
issue: '16'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2924653/
month: '08'
oa: 1
oa_version: Published Version
page: 2700 - 2714
pmid: 1
publication: EMBO Journal
publication_status: published
publisher: Wiley-Blackwell
publist_id: '3629'
quality_controlled: '1'
status: public
title: 'The march of the PINs: Developmental plasticity by dynamic polar targeting
in plant cells'
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 29
year: '2010'
...
---
_id: '3073'
abstract:
- lang: eng
text: Polar membrane cargo delivery is crucial for establishing cell polarity and
for directional transport processes. In plants, polar trafficking mediates the
dynamic asymmetric distribution of PIN FORMED (PIN) carriers, which drive polar
cell-to-cell transport of the hormone auxin, thereby generating auxin maxima and
minima that control development. The Arabidopsis PINOID (PID) protein kinase instructs
apical PIN localization by phosphorylating PINs. Here, we identified the PID homologs
WAG1 and WAG2 as new PIN polarity regulators. We show that the AGC3 kinases PID,
WAG1 and WAG2, and not other plant AGC kinases, instruct recruitment of PINs into
the apical recycling pathway by phosphorylating the middle serine in three conserved
TPRXS(N/S) motifs within the PIN central hydrophilic loop. Our results put forward
a model by which apolarly localized PID, WAG1 and WAG2 phosphorylate PINs at the
plasma membrane after default non-polar PIN secretion, and trigger endocytosis-dependent
apical PIN recycling. This phosphorylation-triggered apical PIN recycling competes
with ARF-GEF GNOM-dependent basal recycling to promote apical PIN localization.
In planta, expression domains of PID, WAG1 and WAG2 correlate with apical localization
of PINs in those cell types, indicating the importance of these kinases for apical
PIN localization. Our data show that by directing polar PIN localization and PIN-mediated
polar auxin transport, the three AGC3 kinases redundantly regulate cotyledon development,
root meristem size and gravitropic response, indicating their involvement in both
programmed and adaptive plant development.
article_processing_charge: No
author:
- first_name: Pankaj
full_name: Dhonukshe, Pankaj
last_name: Dhonukshe
- first_name: Fang
full_name: Huang, Fang
last_name: Huang
- first_name: Carlos
full_name: Galván Ampudia, Carlos
last_name: Galván Ampudia
- first_name: Ari
full_name: Mähönen, Ari
last_name: Mähönen
- first_name: Jürgen
full_name: Kleine Vehn, Jürgen
last_name: Kleine Vehn
- first_name: Jian
full_name: Xu, Jian
last_name: Xu
- first_name: Ab
full_name: Quint, Ab
last_name: Quint
- first_name: Kalika
full_name: Prasad, Kalika
last_name: Prasad
- first_name: Jiřĺ
full_name: Friml, Jiřĺ
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Ben
full_name: Scheres, Ben
last_name: Scheres
- first_name: Remko
full_name: Offringa, Remko
last_name: Offringa
citation:
ama: Dhonukshe P, Huang F, Galván Ampudia C, et al. Plasma membrane-bound AGC3 kinases
phosphorylate PIN auxin carriers at TPRXS(N/S) motifs to direct apical PIN recycling.
Development. 2010;137(19):3245-3255. doi:10.1242/dev.052456
apa: Dhonukshe, P., Huang, F., Galván Ampudia, C., Mähönen, A., Kleine Vehn, J.,
Xu, J., … Offringa, R. (2010). Plasma membrane-bound AGC3 kinases phosphorylate
PIN auxin carriers at TPRXS(N/S) motifs to direct apical PIN recycling. Development.
Company of Biologists. https://doi.org/10.1242/dev.052456
chicago: Dhonukshe, Pankaj, Fang Huang, Carlos Galván Ampudia, Ari Mähönen, Jürgen
Kleine Vehn, Jian Xu, Ab Quint, et al. “Plasma Membrane-Bound AGC3 Kinases Phosphorylate
PIN Auxin Carriers at TPRXS(N/S) Motifs to Direct Apical PIN Recycling.” Development.
Company of Biologists, 2010. https://doi.org/10.1242/dev.052456.
ieee: P. Dhonukshe et al., “Plasma membrane-bound AGC3 kinases phosphorylate
PIN auxin carriers at TPRXS(N/S) motifs to direct apical PIN recycling,” Development,
vol. 137, no. 19. Company of Biologists, pp. 3245–3255, 2010.
ista: Dhonukshe P, Huang F, Galván Ampudia C, Mähönen A, Kleine Vehn J, Xu J, Quint
A, Prasad K, Friml J, Scheres B, Offringa R. 2010. Plasma membrane-bound AGC3
kinases phosphorylate PIN auxin carriers at TPRXS(N/S) motifs to direct apical
PIN recycling. Development. 137(19), 3245–3255.
mla: Dhonukshe, Pankaj, et al. “Plasma Membrane-Bound AGC3 Kinases Phosphorylate
PIN Auxin Carriers at TPRXS(N/S) Motifs to Direct Apical PIN Recycling.” Development,
vol. 137, no. 19, Company of Biologists, 2010, pp. 3245–55, doi:10.1242/dev.052456.
short: P. Dhonukshe, F. Huang, C. Galván Ampudia, A. Mähönen, J. Kleine Vehn, J.
Xu, A. Quint, K. Prasad, J. Friml, B. Scheres, R. Offringa, Development 137 (2010)
3245–3255.
date_created: 2018-12-11T12:01:12Z
date_published: 2010-10-01T00:00:00Z
date_updated: 2021-01-12T07:40:52Z
day: '01'
doi: 10.1242/dev.052456
extern: '1'
intvolume: ' 137'
issue: '19'
language:
- iso: eng
month: '10'
oa_version: None
page: 3245 - 3255
publication: Development
publication_status: published
publisher: Company of Biologists
publist_id: '3627'
quality_controlled: '1'
related_material:
link:
- relation: erratum
url: https://doi.org/10.1242/dev.127415
status: public
title: Plasma membrane-bound AGC3 kinases phosphorylate PIN auxin carriers at TPRXS(N/S)
motifs to direct apical PIN recycling
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 137
year: '2010'
...
---
_id: '3074'
abstract:
- lang: eng
text: Auxin is an essential phytohormone that regulates many aspects of plant development.
To identify new genes that function in auxin signaling, we performed a genetic
screen for Arabidopsis thaliana mutants with an alteration in the expression of
the auxin-responsive reporter DR5rev:GFP (for green fluorescent protein). One
of the mutants recovered in this screen, called weak auxin response1 (wxr1), has
a defect in auxin response and exhibits a variety of auxin-related growth defects
in the root. Polar auxin transport is reduced in wxr1 seedlings, resulting in
auxin accumulation in the hypocotyl and cotyledons and a reduction in auxin levels
in the root apex. In addition, the levels of the PIN auxin transport proteins
are reduced in the wxr1 root. We also show that WXR1 is ROOT UV-B SENSITIVE2 (RUS2),
a member of the broadly conserved DUF647 domain protein family found in diverse
eukaryotic organisms. Our data indicate that RUS2/WXR1 is required for auxin transport
and to maintain the normal levels of PIN proteins in the root.
author:
- first_name: Lei
full_name: Ge, Lei
last_name: Ge
- first_name: Wendy
full_name: Peer, Wendy A
last_name: Peer
- first_name: Stéphanie
full_name: Robert, Stéphanie
last_name: Robert
- first_name: Ranjan
full_name: Swarup, Ranjan
last_name: Swarup
- first_name: Songqing
full_name: Ye, Songqing
last_name: Ye
- first_name: Michael
full_name: Prigge, Michael J
last_name: Prigge
- first_name: Jerry
full_name: Cohen, Jerry D
last_name: Cohen
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Angus
full_name: Murphy, Angus S
last_name: Murphy
- first_name: Ding
full_name: Tang, Ding
last_name: Tang
- first_name: Mark
full_name: Estelle, Mark A
last_name: Estelle
citation:
ama: Ge L, Peer W, Robert S, et al. Arabidopsis ROOT UVB SENSITIVE2 WEAK AUXIN RESPONSE1
is required for polar auxin transport. Plant Cell. 2010;22(6):1749-1761.
doi:10.1105/tpc.110.074195
apa: Ge, L., Peer, W., Robert, S., Swarup, R., Ye, S., Prigge, M., … Estelle, M.
(2010). Arabidopsis ROOT UVB SENSITIVE2 WEAK AUXIN RESPONSE1 is required for polar
auxin transport. Plant Cell. American Society of Plant Biologists. https://doi.org/10.1105/tpc.110.074195
chicago: Ge, Lei, Wendy Peer, Stéphanie Robert, Ranjan Swarup, Songqing Ye, Michael
Prigge, Jerry Cohen, et al. “Arabidopsis ROOT UVB SENSITIVE2 WEAK AUXIN RESPONSE1
Is Required for Polar Auxin Transport.” Plant Cell. American Society of
Plant Biologists, 2010. https://doi.org/10.1105/tpc.110.074195.
ieee: L. Ge et al., “Arabidopsis ROOT UVB SENSITIVE2 WEAK AUXIN RESPONSE1
is required for polar auxin transport,” Plant Cell, vol. 22, no. 6. American
Society of Plant Biologists, pp. 1749–1761, 2010.
ista: Ge L, Peer W, Robert S, Swarup R, Ye S, Prigge M, Cohen J, Friml J, Murphy
A, Tang D, Estelle M. 2010. Arabidopsis ROOT UVB SENSITIVE2 WEAK AUXIN RESPONSE1
is required for polar auxin transport. Plant Cell. 22(6), 1749–1761.
mla: Ge, Lei, et al. “Arabidopsis ROOT UVB SENSITIVE2 WEAK AUXIN RESPONSE1 Is Required
for Polar Auxin Transport.” Plant Cell, vol. 22, no. 6, American Society
of Plant Biologists, 2010, pp. 1749–61, doi:10.1105/tpc.110.074195.
short: L. Ge, W. Peer, S. Robert, R. Swarup, S. Ye, M. Prigge, J. Cohen, J. Friml,
A. Murphy, D. Tang, M. Estelle, Plant Cell 22 (2010) 1749–1761.
date_created: 2018-12-11T12:01:13Z
date_published: 2010-06-01T00:00:00Z
date_updated: 2021-01-12T07:40:52Z
day: '01'
doi: 10.1105/tpc.110.074195
extern: 1
intvolume: ' 22'
issue: '6'
month: '06'
page: 1749 - 1761
publication: Plant Cell
publication_status: published
publisher: American Society of Plant Biologists
publist_id: '3628'
quality_controlled: 0
status: public
title: Arabidopsis ROOT UVB SENSITIVE2 WEAK AUXIN RESPONSE1 is required for polar
auxin transport
type: journal_article
volume: 22
year: '2010'
...
---
_id: '3075'
abstract:
- lang: eng
text: |2-
Spatial distribution of the plant hormone auxin regulates multiple aspects of plant development. These self-regulating auxin gradients are established by the action of PIN auxin transporters, whose activity is regulated by their constitutive cycling between the plasma membrane and endosomes. Here, we show that auxin signaling by the auxin receptor AUXIN-BINDING PROTEIN 1 (ABP1) inhibits the clathrin-mediated internalization of PIN proteins. ABP1 acts as a positive factor in clathrin recruitment to the plasma membrane, thereby promoting endocytosis. Auxin binding to ABP1 interferes with this action and leads to the inhibition of clathrin-mediated endocytosis. Our study demonstrates that ABP1 mediates a nontranscriptional auxin signaling that regulates the evolutionarily conserved process of clathrin-mediated endocytosis and suggests that this signaling may be essential for the developmentally important feedback of auxin on its own transport.
author:
- first_name: Stéphanie
full_name: Robert, Stéphanie
last_name: Robert
- first_name: Jürgen
full_name: Kleine-Vehn, Jürgen
last_name: Kleine Vehn
- first_name: Elke
full_name: Barbez, Elke
last_name: Barbez
- first_name: Michael
full_name: Sauer, Michael
last_name: Sauer
- first_name: Tomasz
full_name: Paciorek, Tomasz
last_name: Paciorek
- first_name: Pawel
full_name: Pawel Baster
id: 3028BD74-F248-11E8-B48F-1D18A9856A87
last_name: Baster
- first_name: Steffen
full_name: Vanneste, Steffen
last_name: Vanneste
- first_name: Jing
full_name: Zhang, Jing
last_name: Zhang
- first_name: Sibu
full_name: Sibu Simon
id: 4542EF9A-F248-11E8-B48F-1D18A9856A87
last_name: Simon
orcid: 0000-0002-1998-6741
- first_name: Milada
full_name: Čovanová, Milada
last_name: Čovanová
- first_name: Kenichiro
full_name: Hayashi, Kenichiro
last_name: Hayashi
- first_name: Pankaj
full_name: Dhonukshe, Pankaj
last_name: Dhonukshe
- first_name: Zhenbiao
full_name: Yang, Zhenbiao
last_name: Yang
- first_name: Sebastian
full_name: Bednarek, Sebastian Y
last_name: Bednarek
- first_name: Alan
full_name: Jones, Alan M
last_name: Jones
- first_name: Christian
full_name: Luschnig, Christian
last_name: Luschnig
- first_name: Fernando
full_name: Aniento, Fernando
last_name: Aniento
- first_name: Eva
full_name: Zažímalová, Eva
last_name: Zažímalová
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Robert S, Kleine Vehn J, Barbez E, et al. ABP1 mediates auxin inhibition of
clathrin-dependent endocytosis in Arabidopsis. Cell. 2010;143(1):111-121.
doi:10.1016/j.cell.2010.09.027
apa: Robert, S., Kleine Vehn, J., Barbez, E., Sauer, M., Paciorek, T., Baster, P.,
… Friml, J. (2010). ABP1 mediates auxin inhibition of clathrin-dependent endocytosis
in Arabidopsis. Cell. Cell Press. https://doi.org/10.1016/j.cell.2010.09.027
chicago: Robert, Stéphanie, Jürgen Kleine Vehn, Elke Barbez, Michael Sauer, Tomasz
Paciorek, Pawel Baster, Steffen Vanneste, et al. “ABP1 Mediates Auxin Inhibition
of Clathrin-Dependent Endocytosis in Arabidopsis.” Cell. Cell Press, 2010.
https://doi.org/10.1016/j.cell.2010.09.027.
ieee: S. Robert et al., “ABP1 mediates auxin inhibition of clathrin-dependent
endocytosis in Arabidopsis,” Cell, vol. 143, no. 1. Cell Press, pp. 111–121,
2010.
ista: Robert S, Kleine Vehn J, Barbez E, Sauer M, Paciorek T, Baster P, Vanneste
S, Zhang J, Simon S, Čovanová M, Hayashi K, Dhonukshe P, Yang Z, Bednarek S, Jones
A, Luschnig C, Aniento F, Zažímalová E, Friml J. 2010. ABP1 mediates auxin inhibition
of clathrin-dependent endocytosis in Arabidopsis. Cell. 143(1), 111–121.
mla: Robert, Stéphanie, et al. “ABP1 Mediates Auxin Inhibition of Clathrin-Dependent
Endocytosis in Arabidopsis.” Cell, vol. 143, no. 1, Cell Press, 2010, pp.
111–21, doi:10.1016/j.cell.2010.09.027.
short: S. Robert, J. Kleine Vehn, E. Barbez, M. Sauer, T. Paciorek, P. Baster, S.
Vanneste, J. Zhang, S. Simon, M. Čovanová, K. Hayashi, P. Dhonukshe, Z. Yang,
S. Bednarek, A. Jones, C. Luschnig, F. Aniento, E. Zažímalová, J. Friml, Cell
143 (2010) 111–121.
date_created: 2018-12-11T12:01:13Z
date_published: 2010-10-01T00:00:00Z
date_updated: 2021-01-12T07:40:52Z
day: '01'
doi: 10.1016/j.cell.2010.09.027
extern: 1
intvolume: ' 143'
issue: '1'
month: '10'
page: 111 - 121
publication: Cell
publication_status: published
publisher: Cell Press
publist_id: '3626'
quality_controlled: 0
status: public
title: ABP1 mediates auxin inhibition of clathrin-dependent endocytosis in Arabidopsis
type: journal_article
volume: 143
year: '2010'
...
---
_id: '3076'
abstract:
- lang: eng
text: Auxin is a multifunctional hormone essential for plant development and pattern
formation. A nuclear auxin-signaling system controlling auxin-induced gene expression
is well established, but cytoplasmic auxin signaling, as in its coordination of
cell polarization, is unexplored. We found a cytoplasmic auxin-signaling mechanism
that modulates the interdigitated growth of Arabidopsis leaf epidermal pavement
cells (PCs), which develop interdigitated lobes and indentations to form a puzzle-piece
shape in a two-dimensional plane. PC interdigitation is compromised in leaves
deficient in either auxin biosynthesis or its export mediated by PINFORMED 1 localized
at the lobe tip. Auxin coordinately activates two Rho GTPases, ROP2 and ROP6,
which promote the formation of complementary lobes and indentations, respectively.
Activation of these ROPs by auxin occurs within 30 s and depends on AUXIN-BINDING
PROTEIN 1. These findings reveal Rho GTPase-based auxin-signaling mechanisms,
which modulate the spatial coordination of cell expansion across a field of cells.
author:
- first_name: Tongda
full_name: Xu, Tongda
last_name: Xu
- first_name: Mingzhang
full_name: Wen, Mingzhang
last_name: Wen
- first_name: Shingo
full_name: Nagawa, Shingo
last_name: Nagawa
- first_name: Ying
full_name: Fu, Ying
last_name: Fu
- first_name: Jin
full_name: Chen, Jin-Gui
last_name: Chen
- first_name: Ming
full_name: Wu, Ming-Jing
last_name: Wu
- first_name: Catherine
full_name: Perrot-Rechenmann, Catherine
last_name: Perrot Rechenmann
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Alan
full_name: Jones, Alan M
last_name: Jones
- first_name: Zhenbiao
full_name: Yang, Zhenbiao
last_name: Yang
citation:
ama: Xu T, Wen M, Nagawa S, et al. Cell surface- and Rho GTPase-based auxin signaling
controls cellular interdigitation in Arabidopsis. Cell. 2010;143(1):99-110.
doi:10.1016/j.cell.2010.09.003
apa: Xu, T., Wen, M., Nagawa, S., Fu, Y., Chen, J., Wu, M., … Yang, Z. (2010). Cell
surface- and Rho GTPase-based auxin signaling controls cellular interdigitation
in Arabidopsis. Cell. Cell Press. https://doi.org/10.1016/j.cell.2010.09.003
chicago: Xu, Tongda, Mingzhang Wen, Shingo Nagawa, Ying Fu, Jin Chen, Ming Wu, Catherine
Perrot Rechenmann, Jiří Friml, Alan Jones, and Zhenbiao Yang. “Cell Surface- and
Rho GTPase-Based Auxin Signaling Controls Cellular Interdigitation in Arabidopsis.”
Cell. Cell Press, 2010. https://doi.org/10.1016/j.cell.2010.09.003.
ieee: T. Xu et al., “Cell surface- and Rho GTPase-based auxin signaling controls
cellular interdigitation in Arabidopsis,” Cell, vol. 143, no. 1. Cell Press,
pp. 99–110, 2010.
ista: Xu T, Wen M, Nagawa S, Fu Y, Chen J, Wu M, Perrot Rechenmann C, Friml J, Jones
A, Yang Z. 2010. Cell surface- and Rho GTPase-based auxin signaling controls cellular
interdigitation in Arabidopsis. Cell. 143(1), 99–110.
mla: Xu, Tongda, et al. “Cell Surface- and Rho GTPase-Based Auxin Signaling Controls
Cellular Interdigitation in Arabidopsis.” Cell, vol. 143, no. 1, Cell Press,
2010, pp. 99–110, doi:10.1016/j.cell.2010.09.003.
short: T. Xu, M. Wen, S. Nagawa, Y. Fu, J. Chen, M. Wu, C. Perrot Rechenmann, J.
Friml, A. Jones, Z. Yang, Cell 143 (2010) 99–110.
date_created: 2018-12-11T12:01:14Z
date_published: 2010-10-01T00:00:00Z
date_updated: 2021-01-12T07:40:53Z
day: '01'
doi: 10.1016/j.cell.2010.09.003
extern: 1
intvolume: ' 143'
issue: '1'
month: '10'
page: 99 - 110
publication: Cell
publication_status: published
publisher: Cell Press
publist_id: '3625'
quality_controlled: 0
status: public
title: Cell surface- and Rho GTPase-based auxin signaling controls cellular interdigitation
in Arabidopsis
type: journal_article
volume: 143
year: '2010'
...
---
_id: '3077'
author:
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Angharad
full_name: Jones, Angharad
last_name: Jones
citation:
ama: 'Friml J, Jones A. Endoplasmic reticulum: The rising compartment in auxin biology.
Plant Physiology. 2010;154(2):458-462. doi:10.1104/pp.110.161380'
apa: 'Friml, J., & Jones, A. (2010). Endoplasmic reticulum: The rising compartment
in auxin biology. Plant Physiology. American Society of Plant Biologists.
https://doi.org/10.1104/pp.110.161380'
chicago: 'Friml, Jiří, and Angharad Jones. “Endoplasmic Reticulum: The Rising Compartment
in Auxin Biology.” Plant Physiology. American Society of Plant Biologists,
2010. https://doi.org/10.1104/pp.110.161380.'
ieee: 'J. Friml and A. Jones, “Endoplasmic reticulum: The rising compartment in
auxin biology,” Plant Physiology, vol. 154, no. 2. American Society of
Plant Biologists, pp. 458–462, 2010.'
ista: 'Friml J, Jones A. 2010. Endoplasmic reticulum: The rising compartment in
auxin biology. Plant Physiology. 154(2), 458–462.'
mla: 'Friml, Jiří, and Angharad Jones. “Endoplasmic Reticulum: The Rising Compartment
in Auxin Biology.” Plant Physiology, vol. 154, no. 2, American Society
of Plant Biologists, 2010, pp. 458–62, doi:10.1104/pp.110.161380.'
short: J. Friml, A. Jones, Plant Physiology 154 (2010) 458–462.
date_created: 2018-12-11T12:01:14Z
date_published: 2010-10-01T00:00:00Z
date_updated: 2021-01-12T07:40:53Z
day: '01'
doi: 10.1104/pp.110.161380
extern: '1'
external_id:
pmid:
- '20921163'
intvolume: ' 154'
issue: '2'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pubmed/20921163
month: '10'
oa: 1
oa_version: Published Version
page: 458 - 462
pmid: 1
publication: Plant Physiology
publication_status: published
publisher: American Society of Plant Biologists
publist_id: '3624'
status: public
title: 'Endoplasmic reticulum: The rising compartment in auxin biology'
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 154
year: '2010'
...
---
_id: '3078'
abstract:
- lang: eng
text: Rapid advances in the field of plant biology, especially in plant cell biology,
have created the need for methods that allow the localization of proteins in situ
at subcellular resolution. Although in many cases recombinant proteins with fluorescent
proteins can fulfill this task, antibody-based immunological detection of proteins
is a complementary technique, which avoids the risk of inducing side effects by
a fusion protein, such as misexpression, mistargeting, altered stability, or toxicity.
Moreover, recombinant protein techniques are applicable only to a rather limited
set of model plants. The immunolocalization protocols presented here can be used
to display protein localization patterns in different tissues of various plant
species. This chapter describes a whole mount immunolocalization protocol, which
has been extensively used in Arabidopsis roots and some above-ground tissues,
and that also works in other species. Additionally, for bulky or hard tissue types,
a variation of this protocol for paraffin-embedded sections is given.
alternative_title:
- Methods in Molecular Biology
author:
- first_name: Michael
full_name: Sauer, Michael
last_name: Sauer
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: 'Sauer M, Friml J. Immunolocalization of proteins in plants . In: Hennig L,
Köhler C, eds. Plant Developmental Biology. Vol 655. Humana Press; 2010:253-263.
doi:10.1007/978-1-60761-765-5_17'
apa: Sauer, M., & Friml, J. (2010). Immunolocalization of proteins in plants
. In L. Hennig & C. Köhler (Eds.), Plant Developmental Biology (Vol.
655, pp. 253–263). Humana Press. https://doi.org/10.1007/978-1-60761-765-5_17
chicago: Sauer, Michael, and Jiří Friml. “Immunolocalization of Proteins in Plants
.” In Plant Developmental Biology, edited by Lars Hennig and Claudia Köhler,
655:253–63. Humana Press, 2010. https://doi.org/10.1007/978-1-60761-765-5_17.
ieee: M. Sauer and J. Friml, “Immunolocalization of proteins in plants ,” in Plant
Developmental Biology, vol. 655, L. Hennig and C. Köhler, Eds. Humana Press,
2010, pp. 253–263.
ista: 'Sauer M, Friml J. 2010.Immunolocalization of proteins in plants . In: Plant
Developmental Biology. Methods in Molecular Biology, vol. 655, 253–263.'
mla: Sauer, Michael, and Jiří Friml. “Immunolocalization of Proteins in Plants .”
Plant Developmental Biology, edited by Lars Hennig and Claudia Köhler,
vol. 655, Humana Press, 2010, pp. 253–63, doi:10.1007/978-1-60761-765-5_17.
short: M. Sauer, J. Friml, in:, L. Hennig, C. Köhler (Eds.), Plant Developmental
Biology, Humana Press, 2010, pp. 253–263.
date_created: 2018-12-11T12:01:14Z
date_published: 2010-08-12T00:00:00Z
date_updated: 2021-01-12T07:40:53Z
day: '12'
doi: 10.1007/978-1-60761-765-5_17
editor:
- first_name: Lars
full_name: Hennig, Lars
last_name: Hennig
- first_name: Claudia
full_name: Köhler, Claudia
last_name: Köhler
extern: 1
intvolume: ' 655'
month: '08'
page: 253 - 263
publication: Plant Developmental Biology
publication_status: published
publisher: Humana Press
publist_id: '3623'
quality_controlled: 0
status: public
title: 'Immunolocalization of proteins in plants '
type: book_chapter
volume: 655
year: '2010'
...
---
_id: '3079'
abstract:
- lang: eng
text: Plant development is exceptionally flexible as manifested by its potential
for organogenesis and regeneration, which are processes involving rearrangements
of tissue polarities. Fundamental questions concern how individual cells can polarize
in a coordinated manner to integrate into the multicellular context. In canalization
models, the signaling molecule auxin acts as a polarizing cue, and feedback on
the intercellular auxin flow is key for synchronized polarity rearrangements.
We provide a novel mechanistic framework for canalization, based on up-to-date
experimental data and minimal, biologically plausible assumptions. Our model combines
the intracellular auxin signaling for expression of PINFORMED (PIN) auxin transporters
and the theoretical postulation of extracellular auxin signaling for modulation
of PIN subcellular dynamics. Computer simulations faithfully and robustly recapitulated
the experimentally observed patterns of tissue polarity and asymmetric auxin distribution
during formation and regeneration of vascular systems and during the competitive
regulation of shoot branching by apical dominance. Additionally, our model generated
new predictions that could be experimentally validated, highlighting a mechanistically
conceivable explanation for the PIN polarization and canalization of the auxin
flow in plants.
author:
- first_name: Krzysztof T
full_name: Krzysztof Wabnik
id: 4DE369A4-F248-11E8-B48F-1D18A9856A87
last_name: Wabnik
orcid: 0000-0001-7263-0560
- first_name: Jürgen
full_name: Kleine-Vehn, Jürgen
last_name: Kleine Vehn
- first_name: Jozef
full_name: Balla, Jozef
last_name: Balla
- first_name: Michael
full_name: Sauer, Michael
last_name: Sauer
- first_name: Satoshi
full_name: Naramoto, Satoshi
last_name: Naramoto
- first_name: Vilém
full_name: Reinöhl, Vilém
last_name: Reinöhl
- first_name: Roeland
full_name: Merks, Roeland M
last_name: Merks
- first_name: Willy
full_name: Govaerts, Willy J
last_name: Govaerts
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Wabnik KT, Kleine Vehn J, Balla J, et al. Emergence of tissue polarization
from synergy of intracellular and extracellular auxin signaling. Molecular
Systems Biology. 2010;6. doi:10.1038/msb.2010.103
apa: Wabnik, K. T., Kleine Vehn, J., Balla, J., Sauer, M., Naramoto, S., Reinöhl,
V., … Friml, J. (2010). Emergence of tissue polarization from synergy of intracellular
and extracellular auxin signaling. Molecular Systems Biology. Nature Publishing
Group. https://doi.org/10.1038/msb.2010.103
chicago: Wabnik, Krzysztof T, Jürgen Kleine Vehn, Jozef Balla, Michael Sauer, Satoshi
Naramoto, Vilém Reinöhl, Roeland Merks, Willy Govaerts, and Jiří Friml. “Emergence
of Tissue Polarization from Synergy of Intracellular and Extracellular Auxin Signaling.”
Molecular Systems Biology. Nature Publishing Group, 2010. https://doi.org/10.1038/msb.2010.103.
ieee: K. T. Wabnik et al., “Emergence of tissue polarization from synergy
of intracellular and extracellular auxin signaling,” Molecular Systems Biology,
vol. 6. Nature Publishing Group, 2010.
ista: Wabnik KT, Kleine Vehn J, Balla J, Sauer M, Naramoto S, Reinöhl V, Merks R,
Govaerts W, Friml J. 2010. Emergence of tissue polarization from synergy of intracellular
and extracellular auxin signaling. Molecular Systems Biology. 6.
mla: Wabnik, Krzysztof T., et al. “Emergence of Tissue Polarization from Synergy
of Intracellular and Extracellular Auxin Signaling.” Molecular Systems Biology,
vol. 6, Nature Publishing Group, 2010, doi:10.1038/msb.2010.103.
short: K.T. Wabnik, J. Kleine Vehn, J. Balla, M. Sauer, S. Naramoto, V. Reinöhl,
R. Merks, W. Govaerts, J. Friml, Molecular Systems Biology 6 (2010).
date_created: 2018-12-11T12:01:15Z
date_published: 2010-12-21T00:00:00Z
date_updated: 2021-01-12T07:40:54Z
day: '21'
doi: 10.1038/msb.2010.103
extern: 1
intvolume: ' 6'
month: '12'
publication: Molecular Systems Biology
publication_status: published
publisher: Nature Publishing Group
publist_id: '3622'
quality_controlled: 0
status: public
title: Emergence of tissue polarization from synergy of intracellular and extracellular
auxin signaling
type: journal_article
volume: 6
year: '2010'
...
---
_id: '3080'
abstract:
- lang: eng
text: Auxin is an essential plant-specific regulator of patterning processes that
also controls directional growth of roots and shoots. In response to gravity stimulation,
the PIN3 auxin transporter polarizes to the bottomside of gravity-sensing root
cells, presumably redirecting the auxin flux toward the lower side of the root
and triggering gravitropic bending. By combining live-cell imaging techniques
with pharmacological and genetic approaches, we demonstrate that PIN3 polarization
does not require secretion of de novo synthesized proteins or protein degradation,
but instead involves rapid, transient stimulation of PIN endocytosis, presumably
via a clathrin-dependent pathway. Moreover, gravity-induced PIN3 polarization
requires the activity of the guanine nucleotide exchange factors for ARF GTPases
(ARF-GEF) GNOM-dependent polar-targeting path-ways and might involve endosome-based
PIN3 translocation from one cell side to another. Our data suggest that gravity
perception acts at several instances of PIN3 trafficking, ultimately leading to
the polarization of PIN3, which presumably aligns auxin fluxes with gravity vector
and mediates downstream root gravitropic response.
author:
- first_name: Jürgen
full_name: Kleine-Vehn, Jürgen
last_name: Kleine Vehn
- first_name: Zhaojun
full_name: Ding, Zhaojun
last_name: Ding
- first_name: Angharad
full_name: Jones, Angharad R
last_name: Jones
- first_name: Masao
full_name: Tasaka, Masao
last_name: Tasaka
- first_name: Miyo
full_name: Morita, Miyo T
last_name: Morita
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Kleine Vehn J, Ding Z, Jones A, Tasaka M, Morita M, Friml J. Gravity induced
PIN transcytosis for polarization of auxin fluxes in gravity sensing root cells.
PNAS. 2010;107(51):22344-22349. doi:10.1073/pnas.1013145107
apa: Kleine Vehn, J., Ding, Z., Jones, A., Tasaka, M., Morita, M., & Friml,
J. (2010). Gravity induced PIN transcytosis for polarization of auxin fluxes in
gravity sensing root cells. PNAS. National Academy of Sciences. https://doi.org/10.1073/pnas.1013145107
chicago: Kleine Vehn, Jürgen, Zhaojun Ding, Angharad Jones, Masao Tasaka, Miyo Morita,
and Jiří Friml. “Gravity Induced PIN Transcytosis for Polarization of Auxin Fluxes
in Gravity Sensing Root Cells.” PNAS. National Academy of Sciences, 2010.
https://doi.org/10.1073/pnas.1013145107.
ieee: J. Kleine Vehn, Z. Ding, A. Jones, M. Tasaka, M. Morita, and J. Friml, “Gravity
induced PIN transcytosis for polarization of auxin fluxes in gravity sensing root
cells,” PNAS, vol. 107, no. 51. National Academy of Sciences, pp. 22344–22349,
2010.
ista: Kleine Vehn J, Ding Z, Jones A, Tasaka M, Morita M, Friml J. 2010. Gravity
induced PIN transcytosis for polarization of auxin fluxes in gravity sensing root
cells. PNAS. 107(51), 22344–22349.
mla: Kleine Vehn, Jürgen, et al. “Gravity Induced PIN Transcytosis for Polarization
of Auxin Fluxes in Gravity Sensing Root Cells.” PNAS, vol. 107, no. 51,
National Academy of Sciences, 2010, pp. 22344–49, doi:10.1073/pnas.1013145107.
short: J. Kleine Vehn, Z. Ding, A. Jones, M. Tasaka, M. Morita, J. Friml, PNAS 107
(2010) 22344–22349.
date_created: 2018-12-11T12:01:15Z
date_published: 2010-12-21T00:00:00Z
date_updated: 2021-01-12T07:40:55Z
day: '21'
doi: 10.1073/pnas.1013145107
extern: 1
intvolume: ' 107'
issue: '51'
month: '12'
page: 22344 - 22349
publication: PNAS
publication_status: published
publisher: National Academy of Sciences
publist_id: '3620'
quality_controlled: 0
status: public
title: Gravity induced PIN transcytosis for polarization of auxin fluxes in gravity
sensing root cells
type: journal_article
volume: 107
year: '2010'
...
---
_id: '3081'
abstract:
- lang: eng
text: Endocytosis is crucial for various cellular functions and development of multicellular
organisms. In mammals and yeast, ADP-ribosylation factor (ARF) GTPases, key components
of vesicle formation, and their regulators ARF-guanine nucleotide exchange factors
(GEFs) and ARF-GTPase-activating protein (GAPs) mediate endocytosis. A similar
role has not been established in plants,mainly because of the lack of the canonical
ARF and ARF-GEF components that are involved in endocytosis in other eukaryotes.
In this study, we revealed a regulatory mechanism of endocytosis in plants based
on ARF GTPase activity.Weidentified that ARF-GEFGNOMand ARF-GAP VASCULAR NETWORK
DEFECTIVE 3 (VAN3), both of which are involved in polar auxin transport-dependent
morphogenesis, localize at the plasma membranes as well as in intracellular structures.
Variable angle epifluorescence microscopy revealed that GNOM and VAN3 localize
to partially overlapping discrete foci at the plasmamembranes that are regularly
associated with the endocytic vesicle coat clathrin. Genetic studies revealed
that GNOM and VAN3 activities are required for endocytosis and internalization
of plasma membrane proteins, including PIN-FORMED auxin transporters. These findings
identified ARF GTPase-based regulatory mechanisms for endocytosis in plants. GNOMand
VAN3 previously were proposed to function solely at the recycling endosomes and
trans-Golgi networks, respectively. Therefore our findings uncovered an additional
cellular function of these prominent developmental regulators.
author:
- first_name: Satoshi
full_name: Naramoto, Satoshi
last_name: Naramoto
- first_name: Jürgen
full_name: Kleine-Vehn, Jürgen
last_name: Kleine Vehn
- first_name: Stéphanie
full_name: Robert, Stéphanie
last_name: Robert
- first_name: Masaru
full_name: Fujimoto, Masaru
last_name: Fujimoto
- first_name: Tomoko
full_name: Dainobu, Tomoko
last_name: Dainobu
- first_name: Tomasz
full_name: Paciorek, Tomasz
last_name: Paciorek
- first_name: Takashi
full_name: Ueda, Takashi
last_name: Ueda
- first_name: Akihiko
full_name: Nakano, Akihiko
last_name: Nakano
- first_name: Marc
full_name: Van Montagu, Marc C
last_name: Van Montagu
- first_name: Hiroo
full_name: Fukuda, Hiroo
last_name: Fukuda
- first_name: Jirí
full_name: Jirí Friml
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Naramoto S, Kleine Vehn J, Robert S, et al. ADP ribosylation factor machinery
mediates endocytosis in plant cells. PNAS. 2010;107(50):21890-21895. doi:10.1073/pnas.1016260107
apa: Naramoto, S., Kleine Vehn, J., Robert, S., Fujimoto, M., Dainobu, T., Paciorek,
T., … Friml, J. (2010). ADP ribosylation factor machinery mediates endocytosis
in plant cells. PNAS. National Academy of Sciences. https://doi.org/10.1073/pnas.1016260107
chicago: Naramoto, Satoshi, Jürgen Kleine Vehn, Stéphanie Robert, Masaru Fujimoto,
Tomoko Dainobu, Tomasz Paciorek, Takashi Ueda, et al. “ADP Ribosylation Factor
Machinery Mediates Endocytosis in Plant Cells.” PNAS. National Academy
of Sciences, 2010. https://doi.org/10.1073/pnas.1016260107.
ieee: S. Naramoto et al., “ADP ribosylation factor machinery mediates endocytosis
in plant cells,” PNAS, vol. 107, no. 50. National Academy of Sciences,
pp. 21890–21895, 2010.
ista: Naramoto S, Kleine Vehn J, Robert S, Fujimoto M, Dainobu T, Paciorek T, Ueda
T, Nakano A, Van Montagu M, Fukuda H, Friml J. 2010. ADP ribosylation factor machinery
mediates endocytosis in plant cells. PNAS. 107(50), 21890–21895.
mla: Naramoto, Satoshi, et al. “ADP Ribosylation Factor Machinery Mediates Endocytosis
in Plant Cells.” PNAS, vol. 107, no. 50, National Academy of Sciences,
2010, pp. 21890–95, doi:10.1073/pnas.1016260107.
short: S. Naramoto, J. Kleine Vehn, S. Robert, M. Fujimoto, T. Dainobu, T. Paciorek,
T. Ueda, A. Nakano, M. Van Montagu, H. Fukuda, J. Friml, PNAS 107 (2010) 21890–21895.
date_created: 2018-12-11T12:01:15Z
date_published: 2010-12-14T00:00:00Z
date_updated: 2021-01-12T07:40:55Z
day: '14'
doi: 10.1073/pnas.1016260107
extern: 1
intvolume: ' 107'
issue: '50'
month: '12'
page: 21890 - 21895
publication: PNAS
publication_status: published
publisher: National Academy of Sciences
publist_id: '3621'
quality_controlled: 0
status: public
title: ADP ribosylation factor machinery mediates endocytosis in plant cells
type: journal_article
volume: 107
year: '2010'
...
---
_id: '3146'
abstract:
- lang: eng
text: 'Coordinated migration of newly born neurons to their prospective target laminae
is a prerequisite for neural circuit assembly in the developing brain. The evolutionarily
conserved LIS1/NDEL1 complex is essential for neuronal migration in the mammalian
cerebral cortex. The cytoplasmic nature of LIS1 and NDEL1 proteins suggest that
they regulate neuronal migration cell autonomously. Here, we extend mosaic analysis
with double markers (MADM) to mouse chromosome 11 where Lis1, Ndel1, and 14-3-3e{open}
(encoding a LIS1/NDEL1 signaling partner) are located. Analyses of sparse and
uniquely labeled mutant cells in mosaic animals reveal distinct cell-autonomous
functions for these three genes. Lis1 regulates neuronal migration efficiency
in a dose-dependent manner, while Ndel1 is essential for a specific, previously
uncharacterized, late step of neuronal migration: entry into the target lamina.
Comparisons with previous genetic perturbations of Lis1 and Ndel1 also suggest
a surprising degree of cell-nonautonomous function for these proteins in regulating
neuronal migration.'
author:
- first_name: Simon
full_name: Simon Hippenmeyer
id: 37B36620-F248-11E8-B48F-1D18A9856A87
last_name: Hippenmeyer
orcid: 0000-0003-2279-1061
- first_name: Yong
full_name: Youn, Yong H
last_name: Youn
- first_name: Hyang
full_name: Moon, Hyang M
last_name: Moon
- first_name: Kazunari
full_name: Miyamichi, Kazunari
last_name: Miyamichi
- first_name: Hui
full_name: Zong, Hui
last_name: Zong
- first_name: Anthony
full_name: Wynshaw-Boris, Anthony
last_name: Wynshaw Boris
- first_name: Liqun
full_name: Luo, Liqun
last_name: Luo
citation:
ama: Hippenmeyer S, Youn Y, Moon H, et al. Genetic mosaic dissection of Lis1 and
Ndel1 in neuronal migration. Neuron. 2010;68(4):695-709. doi:10.1016/j.neuron.2010.09.027
apa: Hippenmeyer, S., Youn, Y., Moon, H., Miyamichi, K., Zong, H., Wynshaw Boris,
A., & Luo, L. (2010). Genetic mosaic dissection of Lis1 and Ndel1 in neuronal
migration. Neuron. Elsevier. https://doi.org/10.1016/j.neuron.2010.09.027
chicago: Hippenmeyer, Simon, Yong Youn, Hyang Moon, Kazunari Miyamichi, Hui Zong,
Anthony Wynshaw Boris, and Liqun Luo. “Genetic Mosaic Dissection of Lis1 and Ndel1
in Neuronal Migration.” Neuron. Elsevier, 2010. https://doi.org/10.1016/j.neuron.2010.09.027.
ieee: S. Hippenmeyer et al., “Genetic mosaic dissection of Lis1 and Ndel1
in neuronal migration,” Neuron, vol. 68, no. 4. Elsevier, pp. 695–709,
2010.
ista: Hippenmeyer S, Youn Y, Moon H, Miyamichi K, Zong H, Wynshaw Boris A, Luo L.
2010. Genetic mosaic dissection of Lis1 and Ndel1 in neuronal migration. Neuron.
68(4), 695–709.
mla: Hippenmeyer, Simon, et al. “Genetic Mosaic Dissection of Lis1 and Ndel1 in
Neuronal Migration.” Neuron, vol. 68, no. 4, Elsevier, 2010, pp. 695–709,
doi:10.1016/j.neuron.2010.09.027.
short: S. Hippenmeyer, Y. Youn, H. Moon, K. Miyamichi, H. Zong, A. Wynshaw Boris,
L. Luo, Neuron 68 (2010) 695–709.
date_created: 2018-12-11T12:01:39Z
date_published: 2010-11-18T00:00:00Z
date_updated: 2021-01-12T07:41:22Z
day: '18'
doi: 10.1016/j.neuron.2010.09.027
extern: 1
intvolume: ' 68'
issue: '4'
month: '11'
page: 695 - 709
publication: Neuron
publication_status: published
publisher: Elsevier
publist_id: '3550'
quality_controlled: 0
status: public
title: Genetic mosaic dissection of Lis1 and Ndel1 in neuronal migration
type: journal_article
volume: 68
year: '2010'
...
---
_id: '3153'
abstract:
- lang: eng
text: Human immune cells have to penetrate an endothelial barrier during their beneficial
pursuit of infection and their destructive infiltration of tissues in autoimmune
diseases. This transmigration requires Rap1 GTPase to activate integrin affinity.
We define a new model system for this process by demonstrating, with live imaging
and genetics, that during embryonic development Drosophila melanogaster immune
cells penetrate an epithelial, Drosophila E-cadherin (DE-cadherin)-based tissue
barrier. A mutant in RhoL, a GTPase homologue that is specifically expressed in
haemocytes, blocks this invasive step but not other aspects of guided migration.
RhoL mediates integrin adhesion caused by Drosophila Rap1 overexpression and moves
Rap1 away from a concentration in the cytoplasm to the leading edge during invasive
migration. These findings indicate that a programmed migratory step during Drosophila
development bears striking molecular similarities to vertebrate immune cell transmigration
during inflammation, and identify RhoL as a new regulator of invasion, adhesion
and Rap1 localization. Our work establishes the utility of Drosophila for identifying
novel components of immune cell transmigration and for understanding the in vivo
interplay of immune cells with the barriers they penetrate.
author:
- first_name: Daria E
full_name: Daria Siekhaus
id: 3D224B9E-F248-11E8-B48F-1D18A9856A87
last_name: Siekhaus
orcid: 0000-0001-8323-8353
- first_name: Martin
full_name: Haesemeyer, Martin
last_name: Haesemeyer
- first_name: Olivia
full_name: Moffitt, Olivia
last_name: Moffitt
- first_name: Ruth
full_name: Lehmann, Ruth
last_name: Lehmann
citation:
ama: Siekhaus DE, Haesemeyer M, Moffitt O, Lehmann R. RhoL controls invasion and
Rap1 localization during immune cell transmigration in Drosophila. Nature Cell
Biology. 2010;12(6):605-610.
apa: Siekhaus, D. E., Haesemeyer, M., Moffitt, O., & Lehmann, R. (2010). RhoL
controls invasion and Rap1 localization during immune cell transmigration in Drosophila.
Nature Cell Biology. Nature Publishing Group.
chicago: Siekhaus, Daria E, Martin Haesemeyer, Olivia Moffitt, and Ruth Lehmann.
“RhoL Controls Invasion and Rap1 Localization during Immune Cell Transmigration
in Drosophila.” Nature Cell Biology. Nature Publishing Group, 2010.
ieee: D. E. Siekhaus, M. Haesemeyer, O. Moffitt, and R. Lehmann, “RhoL controls
invasion and Rap1 localization during immune cell transmigration in Drosophila,”
Nature Cell Biology, vol. 12, no. 6. Nature Publishing Group, pp. 605–610,
2010.
ista: Siekhaus DE, Haesemeyer M, Moffitt O, Lehmann R. 2010. RhoL controls invasion
and Rap1 localization during immune cell transmigration in Drosophila. Nature
Cell Biology. 12(6), 605–610.
mla: Siekhaus, Daria E., et al. “RhoL Controls Invasion and Rap1 Localization during
Immune Cell Transmigration in Drosophila.” Nature Cell Biology, vol. 12,
no. 6, Nature Publishing Group, 2010, pp. 605–10.
short: D.E. Siekhaus, M. Haesemeyer, O. Moffitt, R. Lehmann, Nature Cell Biology
12 (2010) 605–610.
date_created: 2018-12-11T12:01:42Z
date_published: 2010-06-01T00:00:00Z
date_updated: 2021-01-12T07:41:25Z
day: '01'
extern: 1
intvolume: ' 12'
issue: '6'
main_file_link:
- open_access: '0'
url: 10.1038/ncb2063 PubMed
month: '06'
page: 605 - 610
publication: Nature Cell Biology
publication_status: published
publisher: Nature Publishing Group
publist_id: '3542'
quality_controlled: 0
status: public
title: RhoL controls invasion and Rap1 localization during immune cell transmigration
in Drosophila
type: journal_article
volume: 12
year: '2010'
...
---
_id: '3201'
abstract:
- lang: eng
text: 'The problem of cosegmentation consists of segmenting the same object (or
objects of the same class) in two or more distinct images. Recently a number of
different models have been proposed for this problem. However, no comparison of
such models and corresponding optimization techniques has been done so far. We
analyze three existing models: the L1 norm model of Rother et al. [1], the L2
norm model of Mukherjee et al. [2] and the "reward" model of Hochbaum
and Singh [3]. We also study a new model, which is a straightforward extension
of the Boykov-Jolly model for single image segmentation [4]. In terms of optimization,
we use a Dual Decomposition (DD) technique in addition to optimization methods
in [1,2]. Experiments show a significant improvement of DD over published methods.
Our main conclusion, however, is that the new model is the best overall because
it: (i) has fewest parameters; (ii) is most robust in practice, and (iii) can
be optimized well with an efficient EM-style procedure.'
alternative_title:
- LNCS
author:
- first_name: Sara
full_name: Vicente, Sara
last_name: Vicente
- first_name: Vladimir
full_name: Vladimir Kolmogorov
id: 3D50B0BA-F248-11E8-B48F-1D18A9856A87
last_name: Kolmogorov
- first_name: Carsten
full_name: Rother, Carsten
last_name: Rother
citation:
ama: 'Vicente S, Kolmogorov V, Rother C. Cosegmentation revisited: Models and optimization.
In: Vol 6312. Springer; 2010:465-479. doi:10.1007/978-3-642-15552-9_34'
apa: 'Vicente, S., Kolmogorov, V., & Rother, C. (2010). Cosegmentation revisited:
Models and optimization (Vol. 6312, pp. 465–479). Presented at the ECCV: European
Conference on Computer Vision, Springer. https://doi.org/10.1007/978-3-642-15552-9_34'
chicago: 'Vicente, Sara, Vladimir Kolmogorov, and Carsten Rother. “Cosegmentation
Revisited: Models and Optimization,” 6312:465–79. Springer, 2010. https://doi.org/10.1007/978-3-642-15552-9_34.'
ieee: 'S. Vicente, V. Kolmogorov, and C. Rother, “Cosegmentation revisited: Models
and optimization,” presented at the ECCV: European Conference on Computer Vision,
2010, vol. 6312, pp. 465–479.'
ista: 'Vicente S, Kolmogorov V, Rother C. 2010. Cosegmentation revisited: Models
and optimization. ECCV: European Conference on Computer Vision, LNCS, vol. 6312,
465–479.'
mla: 'Vicente, Sara, et al. Cosegmentation Revisited: Models and Optimization.
Vol. 6312, Springer, 2010, pp. 465–79, doi:10.1007/978-3-642-15552-9_34.'
short: S. Vicente, V. Kolmogorov, C. Rother, in:, Springer, 2010, pp. 465–479.
conference:
name: 'ECCV: European Conference on Computer Vision'
date_created: 2018-12-11T12:01:59Z
date_published: 2010-08-30T00:00:00Z
date_updated: 2021-01-12T07:41:46Z
day: '30'
doi: 10.1007/978-3-642-15552-9_34
extern: 1
intvolume: ' 6312'
main_file_link:
- open_access: '0'
url: http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.330.6803df
month: '08'
page: 465 - 479
publication_status: published
publisher: Springer
publist_id: '3479'
quality_controlled: 0
status: public
title: 'Cosegmentation revisited: Models and optimization'
type: conference
volume: 6312
year: '2010'
...
---
_id: '3202'
abstract:
- lang: eng
text: 'We consider the following problem: given an undirected weighted graph G =
(V,E,c) with nonnegative weights, minimize function c(δ(Π))- λ|Π| for all values
of parameter λ. Here Π is a partition of the set of nodes, the first term is the
cost of edges whose endpoints belong to different components of the partition,
and |Π| is the number of components. The current best known algorithm for this
problem has complexity O(|V| 2) maximum flow computations. We improve it to |V|
parametric maximum flow computations. We observe that the complexity can be improved
further for families of graphs which admit a good separator, e.g. for planar graphs.'
author:
- first_name: Vladimir
full_name: Vladimir Kolmogorov
id: 3D50B0BA-F248-11E8-B48F-1D18A9856A87
last_name: Kolmogorov
citation:
ama: Kolmogorov V. A faster algorithm for computing the principal sequence of partitions
of a graph. Algorithmica. 2010;56(4):394-412. doi:10.1007/s00453-008-9177-z
apa: Kolmogorov, V. (2010). A faster algorithm for computing the principal sequence
of partitions of a graph. Algorithmica. Springer. https://doi.org/10.1007/s00453-008-9177-z
chicago: Kolmogorov, Vladimir. “A Faster Algorithm for Computing the Principal Sequence
of Partitions of a Graph.” Algorithmica. Springer, 2010. https://doi.org/10.1007/s00453-008-9177-z.
ieee: V. Kolmogorov, “A faster algorithm for computing the principal sequence of
partitions of a graph,” Algorithmica, vol. 56, no. 4. Springer, pp. 394–412,
2010.
ista: Kolmogorov V. 2010. A faster algorithm for computing the principal sequence
of partitions of a graph. Algorithmica. 56(4), 394–412.
mla: Kolmogorov, Vladimir. “A Faster Algorithm for Computing the Principal Sequence
of Partitions of a Graph.” Algorithmica, vol. 56, no. 4, Springer, 2010,
pp. 394–412, doi:10.1007/s00453-008-9177-z.
short: V. Kolmogorov, Algorithmica 56 (2010) 394–412.
date_created: 2018-12-11T12:01:59Z
date_published: 2010-04-01T00:00:00Z
date_updated: 2021-01-12T07:41:46Z
day: '01'
doi: 10.1007/s00453-008-9177-z
extern: 1
intvolume: ' 56'
issue: '4'
month: '04'
page: 394 - 412
publication: Algorithmica
publication_status: published
publisher: Springer
publist_id: '3480'
quality_controlled: 0
status: public
title: A faster algorithm for computing the principal sequence of partitions of a
graph
type: journal_article
volume: 56
year: '2010'
...
---
_id: '3233'
abstract:
- lang: eng
text: We present a general parallel-repetition theorem with an efficient reduction.
As a corollary of this theorem we establish that parallel repetition reduces the
soundness error at an exponential rate in any public-coin argument, and more generally,
any argument where the verifier's messages, but not necessarily its decision to
accept or reject, can be efficiently simulated with noticeable probability.
alternative_title:
- LNCS
author:
- first_name: Johan
full_name: Håstad, Johan
last_name: Håstad
- first_name: Rafael
full_name: Pass, Rafael
last_name: Pass
- first_name: Douglas
full_name: Wikström, Douglas
last_name: Wikström
- first_name: Krzysztof Z
full_name: Krzysztof Pietrzak
id: 3E04A7AA-F248-11E8-B48F-1D18A9856A87
last_name: Pietrzak
orcid: 0000-0002-9139-1654
citation:
ama: 'Håstad J, Pass R, Wikström D, Pietrzak KZ. An efficient parallel repetition
theorem. In: Vol 5978. Springer; 2010:1-18. doi:10.1007/978-3-642-11799-2_1'
apa: 'Håstad, J., Pass, R., Wikström, D., & Pietrzak, K. Z. (2010). An efficient
parallel repetition theorem (Vol. 5978, pp. 1–18). Presented at the TCC: Theory
of Cryptography Conference, Springer. https://doi.org/10.1007/978-3-642-11799-2_1'
chicago: Håstad, Johan, Rafael Pass, Douglas Wikström, and Krzysztof Z Pietrzak.
“An Efficient Parallel Repetition Theorem,” 5978:1–18. Springer, 2010. https://doi.org/10.1007/978-3-642-11799-2_1.
ieee: 'J. Håstad, R. Pass, D. Wikström, and K. Z. Pietrzak, “An efficient parallel
repetition theorem,” presented at the TCC: Theory of Cryptography Conference,
2010, vol. 5978, pp. 1–18.'
ista: 'Håstad J, Pass R, Wikström D, Pietrzak KZ. 2010. An efficient parallel repetition
theorem. TCC: Theory of Cryptography Conference, LNCS, vol. 5978, 1–18.'
mla: Håstad, Johan, et al. An Efficient Parallel Repetition Theorem. Vol.
5978, Springer, 2010, pp. 1–18, doi:10.1007/978-3-642-11799-2_1.
short: J. Håstad, R. Pass, D. Wikström, K.Z. Pietrzak, in:, Springer, 2010, pp.
1–18.
conference:
name: 'TCC: Theory of Cryptography Conference'
date_created: 2018-12-11T12:02:10Z
date_published: 2010-03-26T00:00:00Z
date_updated: 2021-01-12T07:41:59Z
day: '26'
doi: 10.1007/978-3-642-11799-2_1
extern: 1
intvolume: ' 5978'
month: '03'
page: 1 - 18
publication_status: published
publisher: Springer
publist_id: '3446'
quality_controlled: 0
status: public
title: An efficient parallel repetition theorem
type: conference
volume: 5978
year: '2010'
...