---
_id: '3505'
abstract:
- lang: eng
text: Cell migration on two-dimensional (2D) substrates follows entirely different
rules than cell migration in three-dimensional (3D) environments. This is especially
relevant for leukocytes that are able to migrate in the absence of adhesion receptors
within the confined geometry of artificial 3D extracellular matrix scaffolds and
within the interstitial space in vivo. Here, we describe in detail a simple and
economical protocol to visualize dendritic cell migration in 3D collagen scaffolds
along chemotactic gradients. This method can be adapted to other cell types and
may serve as a physiologically relevant paradigm for the directed locomotion of
most amoeboid cells.
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
article_type: original
author:
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
- first_name: Tim
full_name: Lämmermann, Tim
last_name: Lämmermann
citation:
ama: Sixt MK, Lämmermann T. In vitro analysis of chemotactic leukocyte migration
in 3D environments. Cell Migration. 2011;769:149-165. doi:10.1007/978-1-61779-207-6_11
apa: Sixt, M. K., & Lämmermann, T. (2011). In vitro analysis of chemotactic
leukocyte migration in 3D environments. Cell Migration. Springer. https://doi.org/10.1007/978-1-61779-207-6_11
chicago: Sixt, Michael K, and Tim Lämmermann. “In Vitro Analysis of Chemotactic
Leukocyte Migration in 3D Environments.” Cell Migration. Springer, 2011.
https://doi.org/10.1007/978-1-61779-207-6_11.
ieee: M. K. Sixt and T. Lämmermann, “In vitro analysis of chemotactic leukocyte
migration in 3D environments,” Cell Migration, vol. 769. Springer, pp.
149–165, 2011.
ista: Sixt MK, Lämmermann T. 2011. In vitro analysis of chemotactic leukocyte migration
in 3D environments. Cell Migration. 769, 149–165.
mla: Sixt, Michael K., and Tim Lämmermann. “In Vitro Analysis of Chemotactic Leukocyte
Migration in 3D Environments.” Cell Migration, vol. 769, Springer, 2011,
pp. 149–65, doi:10.1007/978-1-61779-207-6_11.
short: M.K. Sixt, T. Lämmermann, Cell Migration 769 (2011) 149–165.
date_created: 2018-12-11T12:03:41Z
date_published: 2011-05-17T00:00:00Z
date_updated: 2021-01-12T07:43:55Z
day: '17'
department:
- _id: MiSi
doi: 10.1007/978-1-61779-207-6_11
intvolume: ' 769'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://pure.mpg.de/pubman/item/item_3219628_1/component/file_3219630/Sixt%20et%20al..pdf
month: '05'
oa: 1
oa_version: Published Version
page: 149 - 165
publication: Cell Migration
publication_status: published
publisher: Springer
publist_id: '2882'
quality_controlled: '1'
status: public
title: In vitro analysis of chemotactic leukocyte migration in 3D environments
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 769
year: '2011'
...
---
_id: '3724'
abstract:
- lang: eng
text: 'Small photochromic molecules are widespread in nature and serve as switches
for a plethora of light-controlled processes. In a typical photoreceptor, the
different geometries and polarities of the photochrome isomers are tightly coupled
to functionally relevant conformational changes in the proteins. The past decade
has seen extensive efforts to mimic nature and create proteins controlled by synthetic
photochromes in the laboratory. Here, we discuss the role of molecular modeling
to gain a structural understanding of photochromes and to design light-controlled
peptides and proteins. We address several fundamental questions: What are the
molecular structures of photochromes, particularly for metastable isomers that
cannot be addressed experimentally? How are the structures of bistable photoisomers
coupled to the conformational states of peptides and proteins? Can we design light-controlled
proteins rapidly and reliably? After an introduction to the principles of molecular
modeling, we answer these questions by examining systems that range from the size
of isolated photochromes, to that of peptides and large cell surface receptors,
each from its unique computational perspective.'
author:
- first_name: Harald L
full_name: Harald Janovjak
id: 33BA6C30-F248-11E8-B48F-1D18A9856A87
last_name: Janovjak
orcid: 0000-0002-8023-9315
- first_name: Ehud
full_name: Isacoff, Ehud Y
last_name: Isacoff
citation:
ama: 'Janovjak HL, Isacoff E. Structure-based design of light-controlled proteins.
In: Photosensitive Molecules for the Control of Biological Function. Vol
55. Springer; 2011:233-266. doi:10.1007/978-1-61779-031-7_13'
apa: Janovjak, H. L., & Isacoff, E. (2011). Structure-based design of light-controlled
proteins. In Photosensitive Molecules for the Control of Biological Function
(Vol. 55, pp. 233–266). Springer. https://doi.org/10.1007/978-1-61779-031-7_13
chicago: Janovjak, Harald L, and Ehud Isacoff. “Structure-Based Design of Light-Controlled
Proteins.” In Photosensitive Molecules for the Control of Biological Function,
55:233–66. Springer, 2011. https://doi.org/10.1007/978-1-61779-031-7_13.
ieee: H. L. Janovjak and E. Isacoff, “Structure-based design of light-controlled
proteins,” in Photosensitive Molecules for the Control of Biological Function,
vol. 55, Springer, 2011, pp. 233–266.
ista: 'Janovjak HL, Isacoff E. 2011.Structure-based design of light-controlled proteins.
In: Photosensitive Molecules for the Control of Biological Function. vol. 55,
233–266.'
mla: Janovjak, Harald L., and Ehud Isacoff. “Structure-Based Design of Light-Controlled
Proteins.” Photosensitive Molecules for the Control of Biological Function,
vol. 55, Springer, 2011, pp. 233–66, doi:10.1007/978-1-61779-031-7_13.
short: H.L. Janovjak, E. Isacoff, in:, Photosensitive Molecules for the Control
of Biological Function, Springer, 2011, pp. 233–266.
date_created: 2018-12-11T12:04:49Z
date_published: 2011-03-16T00:00:00Z
date_updated: 2021-01-12T07:51:45Z
day: '16'
doi: 10.1007/978-1-61779-031-7_13
extern: 1
intvolume: ' 55'
month: '03'
page: 233 - 266
publication: Photosensitive Molecules for the Control of Biological Function
publication_status: published
publisher: Springer
publist_id: '2504'
quality_controlled: 0
status: public
title: Structure-based design of light-controlled proteins
type: book_chapter
volume: 55
year: '2011'
...
---
_id: '3770'
abstract:
- lang: eng
text: 'The pink dolphin (Inia geoffrensis) is widely distributed along the Amazon
and Orinoco basins, covering an area of approximately 7 million km2. Previous
morphological and genetic studies have proposed the existence of at least two
evolutionary significant units: one distributed across the Orinoco and Amazon
basins and another confined to the Bolivian Amazon. The presence of barriers in
the riverine environment has been suggested to play a significant role in shaping
present-day patterns of ecological and genetic structure for this species. In
the present study, we examined the phylogeographic structure, lineage divergence
time and historical demography using mitochondrial (mt)DNA sequences in different
pink dolphin populations distributed in large and small spatial scales, including
two neighbouring Brazilian Amazon populations. mtDNA control region (CR) analysis
revealed that the Brazilian haplotypes occupy an intermediate position compared
to three previously studied geographic locations: the Colombian Amazon, the Colombian
Orinoco, and the Bolivian Amazon. On a local scale, we have identified a pattern
of maternal isolation between two neighbouring populations from Brazil. Six mtDNA
CR haplotypes were identified in Brazil with no sharing between the two populations,
as well as specific cytochrome b (cyt b) haplotypes identified in each locality.
In addition, we analyzed autosomal microsatellites to investigate male-mediated
gene flow and demographic changes within the study area in Brazil. Data analysis
of 14 microsatellite loci failed to detect significant population subdivision,
suggesting that male-mediated gene flow may maintain homogeneity between these
two locations. Moreover, both mtDNA and microsatellite data indicate a major demographic
collapse within Brazil in the late Pleistocene. Bayesian skyline plots (BSP) of
mtDNA data revealed a stable population for Colombian and Brazilian Amazon lineages
through time, whereas a population decline was demonstrated in the Colombian Orinoco
lineage. Moreover, BSP and Tajima''s D and Fu''s Fs tests revealed a recent population
expansion exclusively in the Bolivian sample. Finally, we estimated that the diversification
of the Inia sp. lineage began in the Late Pliocene (approximately 3.1 Mya) and
continued throughout the Pleistocene.'
article_processing_charge: No
author:
- first_name: Claudia
full_name: Hollatz, Claudia
last_name: Hollatz
- first_name: Sibelle
full_name: Vilaça, Sibelle
last_name: Vilaça
- first_name: Rodrigo A
full_name: Fernandes Redondo, Rodrigo A
id: 409D5C96-F248-11E8-B48F-1D18A9856A87
last_name: Fernandes Redondo
orcid: 0000-0002-5837-2793
- first_name: Míriam
full_name: Marmontel, Míriam
last_name: Marmontel
- first_name: Cyndi
full_name: Baker, Cyndi
last_name: Baker
- first_name: Fabrício
full_name: Santos, Fabrício
last_name: Santos
citation:
ama: Hollatz C, Vilaça S, Fernandes Redondo RA, Marmontel M, Baker C, Santos F.
The Amazon River system as an ecological barrier driving genetic differentiation
of the pink dolphin (Inia geoffrensis). Biological Journal of the Linnean Society.
2011;102(4):812-827. doi:10.1111/j.1095-8312.2011.01616.x
apa: Hollatz, C., Vilaça, S., Fernandes Redondo, R. A., Marmontel, M., Baker, C.,
& Santos, F. (2011). The Amazon River system as an ecological barrier driving
genetic differentiation of the pink dolphin (Inia geoffrensis). Biological
Journal of the Linnean Society. Wiley. https://doi.org/10.1111/j.1095-8312.2011.01616.x
chicago: Hollatz, Claudia, Sibelle Vilaça, Rodrigo A Fernandes Redondo, Míriam Marmontel,
Cyndi Baker, and Fabrício Santos. “The Amazon River System as an Ecological Barrier
Driving Genetic Differentiation of the Pink Dolphin (Inia Geoffrensis).” Biological
Journal of the Linnean Society. Wiley, 2011. https://doi.org/10.1111/j.1095-8312.2011.01616.x.
ieee: C. Hollatz, S. Vilaça, R. A. Fernandes Redondo, M. Marmontel, C. Baker, and
F. Santos, “The Amazon River system as an ecological barrier driving genetic differentiation
of the pink dolphin (Inia geoffrensis),” Biological Journal of the Linnean
Society, vol. 102, no. 4. Wiley, pp. 812–827, 2011.
ista: Hollatz C, Vilaça S, Fernandes Redondo RA, Marmontel M, Baker C, Santos F.
2011. The Amazon River system as an ecological barrier driving genetic differentiation
of the pink dolphin (Inia geoffrensis). Biological Journal of the Linnean Society.
102(4), 812–827.
mla: Hollatz, Claudia, et al. “The Amazon River System as an Ecological Barrier
Driving Genetic Differentiation of the Pink Dolphin (Inia Geoffrensis).” Biological
Journal of the Linnean Society, vol. 102, no. 4, Wiley, 2011, pp. 812–27,
doi:10.1111/j.1095-8312.2011.01616.x.
short: C. Hollatz, S. Vilaça, R.A. Fernandes Redondo, M. Marmontel, C. Baker, F.
Santos, Biological Journal of the Linnean Society 102 (2011) 812–827.
date_created: 2018-12-11T12:05:04Z
date_published: 2011-04-01T00:00:00Z
date_updated: 2021-01-12T07:52:05Z
day: '01'
doi: 10.1111/j.1095-8312.2011.01616.x
extern: '1'
intvolume: ' 102'
issue: '4'
language:
- iso: eng
month: '04'
oa_version: None
page: 812 - 827
publication: Biological Journal of the Linnean Society
publication_status: published
publisher: Wiley
publist_id: '2457'
status: public
title: The Amazon River system as an ecological barrier driving genetic differentiation
of the pink dolphin (Inia geoffrensis)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 102
year: '2011'
...
---
_id: '3771'
abstract:
- lang: eng
text: The small-sized frugivorous bat Carollia perspicillata is an understory specialist
and occurs in a wide range of lowland habitats, tending to be more common in tropical
dry or moist forests of South and Central America. Its sister species, Carollia
brevicauda, occurs almost exclusively in the Amazon rainforest. A recent phylogeographic
study proposed a hypothesis of origin and subsequent diversification for C. perspicillata
along the Atlantic coastal forest of Brazil. Additionally, it also found two allopatric
clades for C. brevicauda separated by the Amazon Basin. We used cytochrome b gene
sequences and a more extensive sampling to test hypotheses related to the origin
and diversification of C. perspicillata plus C. brevicauda clade in South America.
The results obtained indicate that there are two sympatric evolutionary lineages
within each species. In C. perspicillata, one lineage is limited to the Southern
Atlantic Forest, whereas the other is widely distributed. Coalescent analysis
points to a simultaneous origin for C. perspicillata and C. brevicauda, although
no place for the diversification of each species can be firmly suggested. The
phylogeographic pattern shown by C. perspicillata is also congruent with the Pleistocene
refugia hypothesis as a likely vicariant phenomenon shaping the present distribution
of its intraspecific lineages.
author:
- first_name: Ana
full_name: Pavan, Ana
last_name: Pavan
- first_name: Felipe
full_name: Martins, Felipe
last_name: Martins
- first_name: Fabrício
full_name: Santos, Fabrício
last_name: Santos
- first_name: Albert
full_name: Ditchfield, Albert
last_name: Ditchfield
- first_name: Rodrigo A
full_name: Fernandes Redondo, Rodrigo A
id: 409D5C96-F248-11E8-B48F-1D18A9856A87
last_name: Fernandes Redondo
orcid: 0000-0002-5837-2793
citation:
ama: 'Pavan A, Martins F, Santos F, Ditchfield A, Fernandes Redondo RA. Patterns
of diversification in two species of short-tailed bats (Carollia Gray, 1838):
the effects of historical fragmentation of Brazilian rainforests. Biological
Journal of the Linnean Society. 2011;102(3):527-539. doi:10.1111/j.1095-8312.2010.01601.x'
apa: 'Pavan, A., Martins, F., Santos, F., Ditchfield, A., & Fernandes Redondo,
R. A. (2011). Patterns of diversification in two species of short-tailed bats
(Carollia Gray, 1838): the effects of historical fragmentation of Brazilian rainforests.
Biological Journal of the Linnean Society. Wiley-Blackwell. https://doi.org/10.1111/j.1095-8312.2010.01601.x'
chicago: 'Pavan, Ana, Felipe Martins, Fabrício Santos, Albert Ditchfield, and Rodrigo
A Fernandes Redondo. “Patterns of Diversification in Two Species of Short-Tailed
Bats (Carollia Gray, 1838): The Effects of Historical Fragmentation of Brazilian
Rainforests.” Biological Journal of the Linnean Society. Wiley-Blackwell,
2011. https://doi.org/10.1111/j.1095-8312.2010.01601.x.'
ieee: 'A. Pavan, F. Martins, F. Santos, A. Ditchfield, and R. A. Fernandes Redondo,
“Patterns of diversification in two species of short-tailed bats (Carollia Gray,
1838): the effects of historical fragmentation of Brazilian rainforests.,” Biological
Journal of the Linnean Society, vol. 102, no. 3. Wiley-Blackwell, pp. 527–539,
2011.'
ista: 'Pavan A, Martins F, Santos F, Ditchfield A, Fernandes Redondo RA. 2011. Patterns
of diversification in two species of short-tailed bats (Carollia Gray, 1838):
the effects of historical fragmentation of Brazilian rainforests. Biological Journal
of the Linnean Society. 102(3), 527–539.'
mla: 'Pavan, Ana, et al. “Patterns of Diversification in Two Species of Short-Tailed
Bats (Carollia Gray, 1838): The Effects of Historical Fragmentation of Brazilian
Rainforests.” Biological Journal of the Linnean Society, vol. 102, no.
3, Wiley-Blackwell, 2011, pp. 527–39, doi:10.1111/j.1095-8312.2010.01601.x.'
short: A. Pavan, F. Martins, F. Santos, A. Ditchfield, R.A. Fernandes Redondo, Biological
Journal of the Linnean Society 102 (2011) 527–539.
date_created: 2018-12-11T12:05:05Z
date_published: 2011-02-10T00:00:00Z
date_updated: 2021-01-12T07:52:05Z
day: '10'
department:
- _id: FyKo
doi: 10.1111/j.1095-8312.2010.01601.x
intvolume: ' 102'
issue: '3'
language:
- iso: eng
month: '02'
oa_version: None
page: 527 - 539
publication: Biological Journal of the Linnean Society
publication_status: published
publisher: Wiley-Blackwell
publist_id: '2456'
quality_controlled: '1'
scopus_import: 1
status: public
title: 'Patterns of diversification in two species of short-tailed bats (Carollia
Gray, 1838): the effects of historical fragmentation of Brazilian rainforests.'
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 102
year: '2011'
...
---
_id: '3778'
author:
- first_name: Nicholas H
full_name: Barton, Nicholas H
id: 4880FE40-F248-11E8-B48F-1D18A9856A87
last_name: Barton
orcid: 0000-0002-8548-5240
citation:
ama: Barton NH. Estimating linkage disequilibria. Heredity. 2011;106(2):205-206.
doi:10.1038/hdy.2010.67
apa: Barton, N. H. (2011). Estimating linkage disequilibria. Heredity. Nature
Publishing Group. https://doi.org/10.1038/hdy.2010.67
chicago: Barton, Nicholas H. “Estimating Linkage Disequilibria.” Heredity.
Nature Publishing Group, 2011. https://doi.org/10.1038/hdy.2010.67.
ieee: N. H. Barton, “Estimating linkage disequilibria,” Heredity, vol. 106,
no. 2. Nature Publishing Group, pp. 205–206, 2011.
ista: Barton NH. 2011. Estimating linkage disequilibria. Heredity. 106(2), 205–206.
mla: Barton, Nicholas H. “Estimating Linkage Disequilibria.” Heredity, vol.
106, no. 2, Nature Publishing Group, 2011, pp. 205–06, doi:10.1038/hdy.2010.67.
short: N.H. Barton, Heredity 106 (2011) 205–206.
date_created: 2018-12-11T12:05:07Z
date_published: 2011-02-01T00:00:00Z
date_updated: 2021-01-12T07:52:08Z
day: '01'
department:
- _id: NiBa
doi: 10.1038/hdy.2010.67
external_id:
pmid:
- '20502479'
intvolume: ' 106'
issue: '2'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3183869/
month: '02'
oa: 1
oa_version: Submitted Version
page: 205 - 206
pmid: 1
publication: Heredity
publication_status: published
publisher: Nature Publishing Group
publist_id: '2449'
scopus_import: 1
status: public
title: Estimating linkage disequilibria
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 106
year: '2011'
...
---
_id: '3781'
abstract:
- lang: eng
text: We bound the difference in length of two curves in terms of their total curvatures
and the Fréchet distance. The bound is independent of the dimension of the ambient
Euclidean space, it improves upon a bound by Cohen-Steiner and Edelsbrunner, and
it generalizes a result by Fáry and Chakerian.
acknowledgement: Funded by Graduate Aid in Areas of National Need (GAANN) Fellowship.
author:
- first_name: Brittany Terese
full_name: Fasy, Brittany Terese
id: F65D502E-E68D-11E9-9252-C644099818F6
last_name: Fasy
citation:
ama: Fasy BT. The difference in length of curves in R^n. Acta Sci Math (Szeged).
2011;77(1-2):359-367.
apa: Fasy, B. T. (2011). The difference in length of curves in R^n. Acta Sci.
Math. (Szeged). Szegedi Tudományegyetem.
chicago: Fasy, Brittany Terese. “The Difference in Length of Curves in R^n.” Acta
Sci. Math. (Szeged). Szegedi Tudományegyetem, 2011.
ieee: B. T. Fasy, “The difference in length of curves in R^n,” Acta Sci. Math.
(Szeged), vol. 77, no. 1–2. Szegedi Tudományegyetem, pp. 359–367, 2011.
ista: Fasy BT. 2011. The difference in length of curves in R^n. Acta Sci. Math.
(Szeged). 77(1–2), 359–367.
mla: Fasy, Brittany Terese. “The Difference in Length of Curves in R^n.” Acta
Sci. Math. (Szeged), vol. 77, no. 1–2, Szegedi Tudományegyetem, 2011, pp.
359–67.
short: B.T. Fasy, Acta Sci. Math. (Szeged) 77 (2011) 359–367.
date_created: 2018-12-11T12:05:08Z
date_published: 2011-01-01T00:00:00Z
date_updated: 2021-01-12T07:52:09Z
day: '01'
department:
- _id: HeEd
intvolume: ' 77'
issue: 1-2
language:
- iso: eng
month: '01'
oa_version: None
page: 359 - 367
publication: Acta Sci. Math. (Szeged)
publication_status: published
publisher: Szegedi Tudományegyetem
publist_id: '2446'
quality_controlled: '1'
status: public
title: The difference in length of curves in R^n
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 77
year: '2011'
...
---
_id: '3784'
abstract:
- lang: eng
text: Advanced stages of Scyllarus phyllosoma larvae were collected by demersal
trawling during fishery research surveys in the western Mediterranean Sea in 2003–2005.
Nucleotide sequence analysis of the mitochondrial 16S rDNA gene allowed the final-stage
phyllosoma of Scyllarus arctus to be identified among these larvae. Its morphology
is described and illustrated. This constitutes the second complete description
of a Scyllaridae phyllosoma with its specific identity being validated by molecular
techniques (the first was S. pygmaeus). These results also solved a long lasting
taxonomic anomaly of several species assigned to the ancient genus Phyllosoma
Leach, 1814. Detailed examination indicated that the final-stage phyllosoma of
S. arctus shows closer affinities with the American scyllarid Scyllarus depressus
or with the Australian Scyllarus sp. b (sensu Phillips et al., 1981) than to its
sympatric species S. pygmaeus.
article_processing_charge: No
article_type: original
author:
- first_name: Ferran
full_name: Palero, Ferran
id: 3F0E2A22-F248-11E8-B48F-1D18A9856A87
last_name: Palero
orcid: 0000-0002-0343-8329
- first_name: Guillermo
full_name: Guerao, Guillermo
last_name: Guerao
- first_name: Paul
full_name: Clark, Paul
last_name: Clark
- first_name: Pere
full_name: Abello, Pere
last_name: Abello
citation:
ama: 'Palero F, Guerao G, Clark P, Abello P. Scyllarus arctus (Crustacea: Decapoda:
Scyllaridae) final stage phyllosoma identified by DNA analysis, with morphological
description. Journal of the Marine Biological Association of the United Kingdom.
2011;91(2):485-492. doi:10.1017/S0025315410000287'
apa: 'Palero, F., Guerao, G., Clark, P., & Abello, P. (2011). Scyllarus arctus
(Crustacea: Decapoda: Scyllaridae) final stage phyllosoma identified by DNA analysis,
with morphological description. Journal of the Marine Biological Association
of the United Kingdom. Cambridge University Press. https://doi.org/10.1017/S0025315410000287'
chicago: 'Palero, Ferran, Guillermo Guerao, Paul Clark, and Pere Abello. “Scyllarus
Arctus (Crustacea: Decapoda: Scyllaridae) Final Stage Phyllosoma Identified by
DNA Analysis, with Morphological Description.” Journal of the Marine Biological
Association of the United Kingdom. Cambridge University Press, 2011. https://doi.org/10.1017/S0025315410000287.'
ieee: 'F. Palero, G. Guerao, P. Clark, and P. Abello, “Scyllarus arctus (Crustacea:
Decapoda: Scyllaridae) final stage phyllosoma identified by DNA analysis, with
morphological description,” Journal of the Marine Biological Association of
the United Kingdom, vol. 91, no. 2. Cambridge University Press, pp. 485–492,
2011.'
ista: 'Palero F, Guerao G, Clark P, Abello P. 2011. Scyllarus arctus (Crustacea:
Decapoda: Scyllaridae) final stage phyllosoma identified by DNA analysis, with
morphological description. Journal of the Marine Biological Association of the
United Kingdom. 91(2), 485–492.'
mla: 'Palero, Ferran, et al. “Scyllarus Arctus (Crustacea: Decapoda: Scyllaridae)
Final Stage Phyllosoma Identified by DNA Analysis, with Morphological Description.”
Journal of the Marine Biological Association of the United Kingdom, vol.
91, no. 2, Cambridge University Press, 2011, pp. 485–92, doi:10.1017/S0025315410000287.'
short: F. Palero, G. Guerao, P. Clark, P. Abello, Journal of the Marine Biological
Association of the United Kingdom 91 (2011) 485–492.
date_created: 2018-12-11T12:05:09Z
date_published: 2011-03-01T00:00:00Z
date_updated: 2021-01-12T07:52:10Z
day: '01'
department:
- _id: NiBa
doi: 10.1017/S0025315410000287
intvolume: ' 91'
issue: '2'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://digital.csic.es/bitstream/10261/32783/3/Palero_et_al_2011.pdf
month: '03'
oa: 1
oa_version: Published Version
page: 485 - 492
publication: Journal of the Marine Biological Association of the United Kingdom
publication_status: published
publisher: Cambridge University Press
publist_id: '2443'
quality_controlled: '1'
scopus_import: 1
status: public
title: 'Scyllarus arctus (Crustacea: Decapoda: Scyllaridae) final stage phyllosoma
identified by DNA analysis, with morphological description'
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 91
year: '2011'
...
---
_id: '3791'
abstract:
- lang: eng
text: During the development of multicellular organisms, cell fate specification
is followed by the sorting of different cell types into distinct domains from
where the different tissues and organs are formed. Cell sorting involves both
the segregation of a mixed population of cells with different fates and properties
into distinct domains, and the active maintenance of their segregated state. Because
of its biological importance and apparent resemblance to fluid segregation in
physics, cell sorting was extensively studied by both biologists and physicists
over the last decades. Different theories were developed that try to explain cell
sorting on the basis of the physical properties of the constituent cells. However,
only recently the molecular and cellular mechanisms that control the physical
properties driving cell sorting, have begun to be unraveled. In this review, we
will provide an overview of different cell-sorting processes in development and
discuss how these processes can be explained by the different sorting theories,
and how these theories in turn can be connected to the molecular and cellular
mechanisms driving these processes.
alternative_title:
- Current Topics in Developmental Biology
article_processing_charge: No
author:
- first_name: Gabriel
full_name: Krens, Gabriel
id: 2B819732-F248-11E8-B48F-1D18A9856A87
last_name: Krens
orcid: 0000-0003-4761-5996
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: 'Krens G, Heisenberg C-PJ. Cell sorting in development. In: Labouesse M, ed.
Forces and Tension in Development. Vol 95. Elsevier; 2011:189-213. doi:10.1016/B978-0-12-385065-2.00006-2'
apa: Krens, G., & Heisenberg, C.-P. J. (2011). Cell sorting in development.
In M. Labouesse (Ed.), Forces and Tension in Development (Vol. 95, pp.
189–213). Elsevier. https://doi.org/10.1016/B978-0-12-385065-2.00006-2
chicago: Krens, Gabriel, and Carl-Philipp J Heisenberg. “Cell Sorting in Development.”
In Forces and Tension in Development, edited by Michel Labouesse, 95:189–213.
Elsevier, 2011. https://doi.org/10.1016/B978-0-12-385065-2.00006-2.
ieee: G. Krens and C.-P. J. Heisenberg, “Cell sorting in development,” in Forces
and Tension in Development, vol. 95, M. Labouesse, Ed. Elsevier, 2011, pp.
189–213.
ista: 'Krens G, Heisenberg C-PJ. 2011.Cell sorting in development. In: Forces and
Tension in Development. Current Topics in Developmental Biology, vol. 95, 189–213.'
mla: Krens, Gabriel, and Carl-Philipp J. Heisenberg. “Cell Sorting in Development.”
Forces and Tension in Development, edited by Michel Labouesse, vol. 95,
Elsevier, 2011, pp. 189–213, doi:10.1016/B978-0-12-385065-2.00006-2.
short: G. Krens, C.-P.J. Heisenberg, in:, M. Labouesse (Ed.), Forces and Tension
in Development, Elsevier, 2011, pp. 189–213.
date_created: 2018-12-11T12:05:11Z
date_published: 2011-01-01T00:00:00Z
date_updated: 2021-01-12T07:52:13Z
day: '01'
department:
- _id: CaHe
doi: 10.1016/B978-0-12-385065-2.00006-2
editor:
- first_name: Michel
full_name: Labouesse, Michel
last_name: Labouesse
intvolume: ' 95'
language:
- iso: eng
month: '01'
oa_version: None
page: 189 - 213
publication: Forces and Tension in Development
publication_status: published
publisher: Elsevier
publist_id: '2436'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Cell sorting in development
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 95
year: '2011'
...
---
_id: '3796'
abstract:
- lang: eng
text: We address the problem of covering ℝ n with congruent balls, while minimizing
the number of balls that contain an average point. Considering the 1-parameter
family of lattices defined by stretching or compressing the integer grid in diagonal
direction, we give a closed formula for the covering density that depends on the
distortion parameter. We observe that our family contains the thinnest lattice
coverings in dimensions 2 to 5. We also consider the problem of packing congruent
balls in ℝ n , for which we give a closed formula for the packing density as well.
Again we observe that our family contains optimal configurations, this time densest
packings in dimensions 2 and 3.
alternative_title:
- LNCS
author:
- first_name: Herbert
full_name: Edelsbrunner, Herbert
id: 3FB178DA-F248-11E8-B48F-1D18A9856A87
last_name: Edelsbrunner
orcid: 0000-0002-9823-6833
- first_name: Michael
full_name: Kerber, Michael
id: 36E4574A-F248-11E8-B48F-1D18A9856A87
last_name: Kerber
orcid: 0000-0002-8030-9299
citation:
ama: 'Edelsbrunner H, Kerber M. Covering and packing with spheres by diagonal distortion
in R^n. In: Calude C, Rozenberg G, Salomaa A, eds. Rainbow of Computer Science.
Vol 6570. Dedicated to Hermann Maurer on the Occasion of His 70th Birthday. Springer;
2011:20-35. doi:10.1007/978-3-642-19391-0_2'
apa: Edelsbrunner, H., & Kerber, M. (2011). Covering and packing with spheres
by diagonal distortion in R^n. In C. Calude, G. Rozenberg, & A. Salomaa (Eds.),
Rainbow of Computer Science (Vol. 6570, pp. 20–35). Springer. https://doi.org/10.1007/978-3-642-19391-0_2
chicago: Edelsbrunner, Herbert, and Michael Kerber. “Covering and Packing with Spheres
by Diagonal Distortion in R^n.” In Rainbow of Computer Science, edited
by Cristian Calude, Grzegorz Rozenberg, and Arto Salomaa, 6570:20–35. Dedicated
to Hermann Maurer on the Occasion of His 70th Birthday. Springer, 2011. https://doi.org/10.1007/978-3-642-19391-0_2.
ieee: H. Edelsbrunner and M. Kerber, “Covering and packing with spheres by diagonal
distortion in R^n,” in Rainbow of Computer Science, vol. 6570, C. Calude,
G. Rozenberg, and A. Salomaa, Eds. Springer, 2011, pp. 20–35.
ista: 'Edelsbrunner H, Kerber M. 2011.Covering and packing with spheres by diagonal
distortion in R^n. In: Rainbow of Computer Science. LNCS, vol. 6570, 20–35.'
mla: Edelsbrunner, Herbert, and Michael Kerber. “Covering and Packing with Spheres
by Diagonal Distortion in R^n.” Rainbow of Computer Science, edited by
Cristian Calude et al., vol. 6570, Springer, 2011, pp. 20–35, doi:10.1007/978-3-642-19391-0_2.
short: H. Edelsbrunner, M. Kerber, in:, C. Calude, G. Rozenberg, A. Salomaa (Eds.),
Rainbow of Computer Science, Springer, 2011, pp. 20–35.
date_created: 2018-12-11T12:05:13Z
date_published: 2011-05-03T00:00:00Z
date_updated: 2021-01-12T07:52:15Z
day: '03'
ddc:
- '000'
department:
- _id: HeEd
doi: 10.1007/978-3-642-19391-0_2
editor:
- first_name: Cristian
full_name: Calude, Cristian
last_name: Calude
- first_name: Grzegorz
full_name: Rozenberg, Grzegorz
last_name: Rozenberg
- first_name: Arto
full_name: Salomaa, Arto
last_name: Salomaa
file:
- access_level: open_access
checksum: aaf22b4d7bd4277ffe8db532119cf474
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:07:42Z
date_updated: 2020-07-14T12:46:16Z
file_id: '4640'
file_name: IST-2016-539-v1+1_2011-B-01-CoveringPacking.pdf
file_size: 436875
relation: main_file
file_date_updated: 2020-07-14T12:46:16Z
has_accepted_license: '1'
intvolume: ' 6570'
language:
- iso: eng
month: '05'
oa: 1
oa_version: Submitted Version
page: 20 - 35
publication: Rainbow of Computer Science
publication_status: published
publisher: Springer
publist_id: '2427'
pubrep_id: '539'
quality_controlled: '1'
series_title: Dedicated to Hermann Maurer on the Occasion of His 70th Birthday
status: public
title: Covering and packing with spheres by diagonal distortion in R^n
type: book_chapter
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 6570
year: '2011'
...
---
_id: '386'
abstract:
- lang: eng
text: 'We present a detailed study of the local density of states (LDOS) associated
with the surface-state band near a step edge of the strong topological insulator
Bi2Te3 and reveal a one-dimensional bound state that runs parallel to the step
edge and is bound to it at some characteristic distance. This bound state is clearly
observed in the bulk gap region, while it becomes entangled with the oscillations
of the warped surface band at high energy, and with the valence-band states near
the Dirac point. We obtain excellent fits to theoretical predictions [Alpichshev,
2011] that properly incorporate the three-dimensional nature of the problem to
the surface state. Fitting the data at different energies, we can recalculate
the LDOS originating from the Dirac band without the contribution of the bulk
bands or incoherent tunneling effects. '
article_processing_charge: No
article_type: original
arxiv: 1
author:
- first_name: Zhanybek
full_name: Alpichshev, Zhanybek
id: 45E67A2A-F248-11E8-B48F-1D18A9856A87
last_name: Alpichshev
orcid: 0000-0002-7183-5203
- first_name: J G
full_name: Analytis, J G
last_name: Analytis
- first_name: J H
full_name: Chu, J H
last_name: Chu
- first_name: I R
full_name: Fisher, I R
last_name: Fisher
- first_name: A
full_name: Kapitulnik, A
last_name: Kapitulnik
citation:
ama: Alpichshev Z, Analytis JG, Chu JH, Fisher IR, Kapitulnik A. STM imaging of
a bound state along a step on the surface of the topological insulator Bi2Te3.
Physical Review B - Condensed Matter and Materials Physics. 2011;84(4).
doi:10.1103/PhysRevB.84.041104
apa: Alpichshev, Z., Analytis, J. G., Chu, J. H., Fisher, I. R., & Kapitulnik,
A. (2011). STM imaging of a bound state along a step on the surface of the topological
insulator Bi2Te3. Physical Review B - Condensed Matter and Materials Physics.
American Physical Society. https://doi.org/10.1103/PhysRevB.84.041104
chicago: Alpichshev, Zhanybek, J G Analytis, J H Chu, I R Fisher, and A Kapitulnik.
“STM Imaging of a Bound State along a Step on the Surface of the Topological Insulator
Bi2Te3.” Physical Review B - Condensed Matter and Materials Physics. American
Physical Society, 2011. https://doi.org/10.1103/PhysRevB.84.041104.
ieee: Z. Alpichshev, J. G. Analytis, J. H. Chu, I. R. Fisher, and A. Kapitulnik,
“STM imaging of a bound state along a step on the surface of the topological insulator
Bi2Te3,” Physical Review B - Condensed Matter and Materials Physics, vol.
84, no. 4. American Physical Society, 2011.
ista: Alpichshev Z, Analytis JG, Chu JH, Fisher IR, Kapitulnik A. 2011. STM imaging
of a bound state along a step on the surface of the topological insulator Bi2Te3.
Physical Review B - Condensed Matter and Materials Physics. 84(4).
mla: Alpichshev, Zhanybek, et al. “STM Imaging of a Bound State along a Step on
the Surface of the Topological Insulator Bi2Te3.” Physical Review B - Condensed
Matter and Materials Physics, vol. 84, no. 4, American Physical Society, 2011,
doi:10.1103/PhysRevB.84.041104.
short: Z. Alpichshev, J.G. Analytis, J.H. Chu, I.R. Fisher, A. Kapitulnik, Physical
Review B - Condensed Matter and Materials Physics 84 (2011).
date_created: 2018-12-11T11:46:10Z
date_published: 2011-07-21T00:00:00Z
date_updated: 2021-01-12T07:52:44Z
day: '21'
doi: 10.1103/PhysRevB.84.041104
extern: '1'
external_id:
arxiv:
- '1003.2233'
intvolume: ' 84'
issue: '4'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://arxiv.org/abs/1003.2233
month: '07'
oa: 1
oa_version: Preprint
publication: Physical Review B - Condensed Matter and Materials Physics
publication_status: published
publisher: American Physical Society
publist_id: '7443'
quality_controlled: '1'
status: public
title: STM imaging of a bound state along a step on the surface of the topological
insulator Bi2Te3
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 84
year: '2011'
...
---
_id: '3965'
abstract:
- lang: eng
text: The elevation function on a smoothly embedded 2-manifold in R-3 reflects the
multiscale topography of cavities and protrusions as local maxima. The function
has been useful in identifying coarse docking configurations for protein pairs.
Transporting the concept from the smooth to the piecewise linear category, this
paper describes an algorithm for finding all local maxima. While its worst-case
running time is the same as of the algorithm used in prior work, its performance
in practice is orders of magnitudes superior. We cast light on this improvement
by relating the running time to the total absolute Gaussian curvature of the 2-manifold.
author:
- first_name: Bei
full_name: Wang, Bei
last_name: Wang
- first_name: Herbert
full_name: Edelsbrunner, Herbert
id: 3FB178DA-F248-11E8-B48F-1D18A9856A87
last_name: Edelsbrunner
orcid: 0000-0002-9823-6833
- first_name: Dmitriy
full_name: Morozov, Dmitriy
last_name: Morozov
citation:
ama: Wang B, Edelsbrunner H, Morozov D. Computing elevation maxima by searching
the Gauss sphere. Journal of Experimental Algorithmics. 2011;16(2.2):1-13.
doi:10.1145/1963190.1970375
apa: Wang, B., Edelsbrunner, H., & Morozov, D. (2011). Computing elevation maxima
by searching the Gauss sphere. Journal of Experimental Algorithmics. ACM.
https://doi.org/10.1145/1963190.1970375
chicago: Wang, Bei, Herbert Edelsbrunner, and Dmitriy Morozov. “Computing Elevation
Maxima by Searching the Gauss Sphere.” Journal of Experimental Algorithmics.
ACM, 2011. https://doi.org/10.1145/1963190.1970375.
ieee: B. Wang, H. Edelsbrunner, and D. Morozov, “Computing elevation maxima by searching
the Gauss sphere,” Journal of Experimental Algorithmics, vol. 16, no. 2.2.
ACM, pp. 1–13, 2011.
ista: Wang B, Edelsbrunner H, Morozov D. 2011. Computing elevation maxima by searching
the Gauss sphere. Journal of Experimental Algorithmics. 16(2.2), 1–13.
mla: Wang, Bei, et al. “Computing Elevation Maxima by Searching the Gauss Sphere.”
Journal of Experimental Algorithmics, vol. 16, no. 2.2, ACM, 2011, pp.
1–13, doi:10.1145/1963190.1970375.
short: B. Wang, H. Edelsbrunner, D. Morozov, Journal of Experimental Algorithmics
16 (2011) 1–13.
date_created: 2018-12-11T12:06:09Z
date_published: 2011-05-01T00:00:00Z
date_updated: 2021-01-12T07:53:31Z
day: '01'
department:
- _id: HeEd
doi: 10.1145/1963190.1970375
intvolume: ' 16'
issue: '2.2'
language:
- iso: eng
month: '05'
oa_version: None
page: 1 - 13
publication: Journal of Experimental Algorithmics
publication_status: published
publisher: ACM
publist_id: '2161'
quality_controlled: '1'
scopus_import: 1
status: public
title: Computing elevation maxima by searching the Gauss sphere
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 16
year: '2011'
...
---
_id: '2442'
abstract:
- lang: eng
text: In a new study published in this issue of Developmental Cell, Krouk et al.
reveal a surprising mechanism by which plant root systems adapt their architecture
for soil exploitation. The dual transporter NRT1.1 uses both nitrate and the plant
hormone auxin as substrates, enabling soil nitrate availability to regulate auxin-driven
lateral root development.
author:
- first_name: Tom
full_name: Beeckman, Tom
last_name: Beeckman
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: 'Beeckman T, Friml J. Nitrate Contra Auxin: Nutrient Sensing by roots. Developmental
Cell. 2010;18(6):877-878. doi:10.1016/j.devcel.2010.05.020'
apa: 'Beeckman, T., & Friml, J. (2010). Nitrate Contra Auxin: Nutrient Sensing
by roots. Developmental Cell. Cell Press. https://doi.org/10.1016/j.devcel.2010.05.020'
chicago: 'Beeckman, Tom, and Jiří Friml. “Nitrate Contra Auxin: Nutrient Sensing
by Roots.” Developmental Cell. Cell Press, 2010. https://doi.org/10.1016/j.devcel.2010.05.020.'
ieee: 'T. Beeckman and J. Friml, “Nitrate Contra Auxin: Nutrient Sensing by roots,”
Developmental Cell, vol. 18, no. 6. Cell Press, pp. 877–878, 2010.'
ista: 'Beeckman T, Friml J. 2010. Nitrate Contra Auxin: Nutrient Sensing by roots.
Developmental Cell. 18(6), 877–878.'
mla: 'Beeckman, Tom, and Jiří Friml. “Nitrate Contra Auxin: Nutrient Sensing by
Roots.” Developmental Cell, vol. 18, no. 6, Cell Press, 2010, pp. 877–78,
doi:10.1016/j.devcel.2010.05.020.'
short: T. Beeckman, J. Friml, Developmental Cell 18 (2010) 877–878.
date_created: 2018-12-11T11:57:41Z
date_published: 2010-06-15T00:00:00Z
date_updated: 2021-01-12T06:57:31Z
day: '15'
doi: 10.1016/j.devcel.2010.05.020
extern: '1'
external_id:
pmid:
- ' 20627068'
intvolume: ' 18'
issue: '6'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pubmed/20627068
month: '06'
oa: 1
oa_version: Published Version
page: 877 - 878
pmid: 1
publication: Developmental Cell
publication_status: published
publisher: Cell Press
publist_id: '4461'
quality_controlled: '1'
status: public
title: 'Nitrate Contra Auxin: Nutrient Sensing by roots'
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 18
year: '2010'
...
---
_id: '2503'
abstract:
- lang: eng
text: Epilepsy is a devastating and poorly understood disease. Mutations in a secreted
neuronal protein, leucine-rich glioma inactivated 1 (LGI1), were reported in patients
with an inherited form of human epilepsy, autosomal dominant partial epilepsy
with auditory features (ADPEAF). Here, we report an essential role of LGI1 as
an antiepileptogenic ligand. We find that loss of LGI1 in mice (LGI1-/-) causes
lethal epilepsy, which is specifically rescued by the neuronal expression of LGI1
transgene, but not LGI3. Moreover, heterozygous mice for the LGI1 mutation (LGI1+/-)
show lowered seizure thresholds. Extracellularly secreted LGI1 links two epilepsy-related
receptors, ADAM22 and ADAM23, in the brain and organizes a transsynaptic protein
complex that includes presynaptic potassium channels and postsynaptic AMPA receptor
scaffolds. A lack of LGI1 disrupts this synaptic protein connection and selectively
reduces AMPA receptor-mediated synaptic transmission in the hippocampus. Thus,
LGI1 may serve as a major determinant of brain excitation, and the LGI1 gene-targeted
mouse provides a good model for human epilepsy.
author:
- first_name: Yuko
full_name: Fukata, Yuko
last_name: Fukata
- first_name: Kathryn
full_name: Lovero, Kathryn L
last_name: Lovero
- first_name: Tsuyoshi
full_name: Iwanaga, Tsuyoshi
last_name: Iwanaga
- first_name: Atsushi
full_name: Watanabe, Atsushi
last_name: Watanabe
- first_name: Norihiko
full_name: Yokoi, Norihiko
last_name: Yokoi
- first_name: Katsuhiko
full_name: Tabuchi, Katsuhiko
last_name: Tabuchi
- first_name: Ryuichi
full_name: Ryuichi Shigemoto
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Roger
full_name: Nicoll, Roger A
last_name: Nicoll
- first_name: Masaki
full_name: Fukata, Masaki
last_name: Fukata
citation:
ama: Fukata Y, Lovero K, Iwanaga T, et al. Disruption of LGI1-linked synaptic complex
causes abnormal synaptic transmission and epilepsy. PNAS. 2010;107(8):3799-3804.
doi:10.1073/pnas.0914537107
apa: Fukata, Y., Lovero, K., Iwanaga, T., Watanabe, A., Yokoi, N., Tabuchi, K.,
… Fukata, M. (2010). Disruption of LGI1-linked synaptic complex causes abnormal
synaptic transmission and epilepsy. PNAS. National Academy of Sciences.
https://doi.org/10.1073/pnas.0914537107
chicago: Fukata, Yuko, Kathryn Lovero, Tsuyoshi Iwanaga, Atsushi Watanabe, Norihiko
Yokoi, Katsuhiko Tabuchi, Ryuichi Shigemoto, Roger Nicoll, and Masaki Fukata.
“Disruption of LGI1-Linked Synaptic Complex Causes Abnormal Synaptic Transmission
and Epilepsy.” PNAS. National Academy of Sciences, 2010. https://doi.org/10.1073/pnas.0914537107.
ieee: Y. Fukata et al., “Disruption of LGI1-linked synaptic complex causes
abnormal synaptic transmission and epilepsy,” PNAS, vol. 107, no. 8. National
Academy of Sciences, pp. 3799–3804, 2010.
ista: Fukata Y, Lovero K, Iwanaga T, Watanabe A, Yokoi N, Tabuchi K, Shigemoto R,
Nicoll R, Fukata M. 2010. Disruption of LGI1-linked synaptic complex causes abnormal
synaptic transmission and epilepsy. PNAS. 107(8), 3799–3804.
mla: Fukata, Yuko, et al. “Disruption of LGI1-Linked Synaptic Complex Causes Abnormal
Synaptic Transmission and Epilepsy.” PNAS, vol. 107, no. 8, National Academy
of Sciences, 2010, pp. 3799–804, doi:10.1073/pnas.0914537107.
short: Y. Fukata, K. Lovero, T. Iwanaga, A. Watanabe, N. Yokoi, K. Tabuchi, R. Shigemoto,
R. Nicoll, M. Fukata, PNAS 107 (2010) 3799–3804.
date_created: 2018-12-11T11:58:03Z
date_published: 2010-02-23T00:00:00Z
date_updated: 2021-01-12T06:57:53Z
day: '23'
doi: 10.1073/pnas.0914537107
extern: 1
intvolume: ' 107'
issue: '8'
month: '02'
page: 3799 - 3804
publication: PNAS
publication_status: published
publisher: National Academy of Sciences
publist_id: '4398'
quality_controlled: 0
status: public
title: Disruption of LGI1-linked synaptic complex causes abnormal synaptic transmission
and epilepsy
type: journal_article
volume: 107
year: '2010'
...
---
_id: '2504'
abstract:
- lang: eng
text: We present a method for immunolabeling of multiple species of membrane proteins
with high spatial resolution. It allows differentiation of equally sized very
small markers with different chemical compositions, which leads to high labeling
efficiency and reduces steric hindrance of closely spaced immunolabeled biomolecules.
Markers such as CdSe/ZnS semiconductor quantum dots and colloidal gold particles
are distinguished by differential contrast in high-angle annular detector dark-field
STEM mode or by EDX microanalysis of their elemental contents. This method was
tested by observation of labeled AMPA- and NMDA-type glutamate receptors on sodium-dodecyl-sulfate-digested
replica prepared from rat hippocampus. To improve particle visibility and detectability,
the replica films were made exclusively with carbon to avoid the high background
of conventional platinum/carbon replica. Extension of the method is suggested
by detection of 1.4 nm nanogold particles and its potential application in the
biological imaging research.
author:
- first_name: Alexandre
full_name: Loukanov, Alexandre R
last_name: Loukanov
- first_name: Naomi
full_name: Kamasawa, Naomi
last_name: Kamasawa
- first_name: Radostin
full_name: Danev, Radostin S
last_name: Danev
- first_name: Ryuichi
full_name: Ryuichi Shigemoto
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Kuniaki
full_name: Nagayama, Kuniaki
last_name: Nagayama
citation:
ama: Loukanov A, Kamasawa N, Danev R, Shigemoto R, Nagayama K. Immunolocalization
of multiple membrane proteins on a carbon replica with STEM and EDX. Ultramicroscopy.
2010;110(4):366-374. doi:10.1016/j.ultramic.2010.01.016
apa: Loukanov, A., Kamasawa, N., Danev, R., Shigemoto, R., & Nagayama, K. (2010).
Immunolocalization of multiple membrane proteins on a carbon replica with STEM
and EDX. Ultramicroscopy. Elsevier. https://doi.org/10.1016/j.ultramic.2010.01.016
chicago: Loukanov, Alexandre, Naomi Kamasawa, Radostin Danev, Ryuichi Shigemoto,
and Kuniaki Nagayama. “Immunolocalization of Multiple Membrane Proteins on a Carbon
Replica with STEM and EDX.” Ultramicroscopy. Elsevier, 2010. https://doi.org/10.1016/j.ultramic.2010.01.016.
ieee: A. Loukanov, N. Kamasawa, R. Danev, R. Shigemoto, and K. Nagayama, “Immunolocalization
of multiple membrane proteins on a carbon replica with STEM and EDX,” Ultramicroscopy,
vol. 110, no. 4. Elsevier, pp. 366–374, 2010.
ista: Loukanov A, Kamasawa N, Danev R, Shigemoto R, Nagayama K. 2010. Immunolocalization
of multiple membrane proteins on a carbon replica with STEM and EDX. Ultramicroscopy.
110(4), 366–374.
mla: Loukanov, Alexandre, et al. “Immunolocalization of Multiple Membrane Proteins
on a Carbon Replica with STEM and EDX.” Ultramicroscopy, vol. 110, no.
4, Elsevier, 2010, pp. 366–74, doi:10.1016/j.ultramic.2010.01.016.
short: A. Loukanov, N. Kamasawa, R. Danev, R. Shigemoto, K. Nagayama, Ultramicroscopy
110 (2010) 366–374.
date_created: 2018-12-11T11:58:03Z
date_published: 2010-03-01T00:00:00Z
date_updated: 2021-01-12T06:57:53Z
day: '01'
doi: 10.1016/j.ultramic.2010.01.016
extern: 1
intvolume: ' 110'
issue: '4'
month: '03'
page: 366 - 374
publication: Ultramicroscopy
publication_status: published
publisher: Elsevier
publist_id: '4397'
quality_controlled: 0
status: public
title: Immunolocalization of multiple membrane proteins on a carbon replica with STEM
and EDX
type: journal_article
volume: 110
year: '2010'
...
---
_id: '2505'
abstract:
- lang: eng
text: Cbln1, secreted from cerebellar granule cells, and the orphan glutamate receptor
52 (GluD2), expressed by Purkinje cells, are essential for synapse integrity between
these neurons in adult mice. Nevertheless, no endogenous binding partners for
these molecules have been identified. We found that Cblnl binds directly to the
N-terminal domain of GluD2. GluD2 expression by postsynaptic cells, combined with
exogenously applied Cbln1, was necessary and sufficient to induce new synapses
in vitro and in the adult cerebellum in vivo. Further, beads coated with recombinant
Cbln1 directly induced presynaptic differentiation and indirectly caused clustering
of postsynaptic molecules via GluD2. These results indicate that the Cbln1-GluD2
complex is a unique synapse organizer that acts bidirectionally on both pre- and
postsynaptic components.
author:
- first_name: Keiko
full_name: Matsuda, Keiko
last_name: Matsuda
- first_name: Eriko
full_name: Miura, Eriko
last_name: Miura
- first_name: Taisuke
full_name: Miyazaki, Taisuke
last_name: Miyazaki
- first_name: Wataru
full_name: Kakegawa, Wataru
last_name: Kakegawa
- first_name: Kyoichi
full_name: Emi, Kyoichi
last_name: Emi
- first_name: Sakae
full_name: Narumi, Sakae
last_name: Narumi
- first_name: Yugo
full_name: Fukazawa, Yugo
last_name: Fukazawa
- first_name: Aya
full_name: Ito-lshida, Aya
last_name: Ito Lshida
- first_name: Tetsuro
full_name: Kondo, Tetsuro
last_name: Kondo
- first_name: Ryuichi
full_name: Ryuichi Shigemoto
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Masahiko
full_name: Watanabe, Masahiko
last_name: Watanabe
- first_name: Michisuke
full_name: Yuzaki, Michisuke
last_name: Yuzaki
citation:
ama: Matsuda K, Miura E, Miyazaki T, et al. Cbln1 is a ligand for an orphan glutamate
receptor δ2, a bidirectional synapse organizer. Science. 2010;328(5976):363-368.
doi:10.1126/science.1185152
apa: Matsuda, K., Miura, E., Miyazaki, T., Kakegawa, W., Emi, K., Narumi, S., …
Yuzaki, M. (2010). Cbln1 is a ligand for an orphan glutamate receptor δ2, a bidirectional
synapse organizer. Science. American Association for the Advancement of
Science. https://doi.org/10.1126/science.1185152
chicago: Matsuda, Keiko, Eriko Miura, Taisuke Miyazaki, Wataru Kakegawa, Kyoichi
Emi, Sakae Narumi, Yugo Fukazawa, et al. “Cbln1 Is a Ligand for an Orphan Glutamate
Receptor Δ2, a Bidirectional Synapse Organizer.” Science. American Association
for the Advancement of Science, 2010. https://doi.org/10.1126/science.1185152.
ieee: K. Matsuda et al., “Cbln1 is a ligand for an orphan glutamate receptor
δ2, a bidirectional synapse organizer,” Science, vol. 328, no. 5976. American
Association for the Advancement of Science, pp. 363–368, 2010.
ista: Matsuda K, Miura E, Miyazaki T, Kakegawa W, Emi K, Narumi S, Fukazawa Y, Ito
Lshida A, Kondo T, Shigemoto R, Watanabe M, Yuzaki M. 2010. Cbln1 is a ligand
for an orphan glutamate receptor δ2, a bidirectional synapse organizer. Science.
328(5976), 363–368.
mla: Matsuda, Keiko, et al. “Cbln1 Is a Ligand for an Orphan Glutamate Receptor
Δ2, a Bidirectional Synapse Organizer.” Science, vol. 328, no. 5976, American
Association for the Advancement of Science, 2010, pp. 363–68, doi:10.1126/science.1185152.
short: K. Matsuda, E. Miura, T. Miyazaki, W. Kakegawa, K. Emi, S. Narumi, Y. Fukazawa,
A. Ito Lshida, T. Kondo, R. Shigemoto, M. Watanabe, M. Yuzaki, Science 328 (2010)
363–368.
date_created: 2018-12-11T11:58:04Z
date_published: 2010-04-16T00:00:00Z
date_updated: 2021-01-12T06:57:53Z
day: '16'
doi: 10.1126/science.1185152
extern: 1
intvolume: ' 328'
issue: '5976'
month: '04'
page: 363 - 368
publication: Science
publication_status: published
publisher: American Association for the Advancement of Science
publist_id: '4396'
quality_controlled: 0
status: public
title: Cbln1 is a ligand for an orphan glutamate receptor δ2, a bidirectional synapse
organizer
type: journal_article
volume: 328
year: '2010'
...
---
_id: '2506'
abstract:
- lang: eng
text: Subepithelial fibroblasts of the intestinal villi, which form a contractile
cellular network beneath the epithelium, are in close contact with epithelial
cells, nerve varicosities, capillaries, smooth muscles and immune cells, and secrete
extracellular matrix molecules, growth factors and cytokines, etc. Cultured subepithelial
fibroblasts of the rat duodenal villi display various receptors such as endothelins,
ATP, substance-P and bradykinin, and release ATP in response to mechanical stimulation.
In this study, the presence of functional NK1 receptors (NK1R) was pharmacologically
confirmed in primary culture by Ca 2+ measurement, and the effects of substance-P
were measured in an acute preparation of epithelium-free duodenal villi from 2-
to 3-week-old rats using a two-photon laser microscope. Substance-P elicited an
increase in the intracellular Ca 2+ concentration and contraction of the subepithelial
fibroblasts in culture and the isolated villi. The localization of NK1R and substance-P
in the villi was examined by light and electron microscopic immunohistochemistry.
NK1R-like immunoreactivity was intensely localized on the plasma membrane of villous
subepithelial fibroblasts in 10-day- to 4-week-old rats and mice and was decreased
or absent in adulthood. The pericryptal fibroblasts of the small and large intestine
were NK1R immuno-negative. These villous subepithelial fibroblasts form synapse-like
structures with both substance-P-immunopositive and -immunonegative nerve varicosities.
Here, we propose that the mutual interaction between villous subepithelial fibroblasts
and afferent neurons via substance-P and ATP plays important roles in the maturation
of the structure and function of the small intestine.
author:
- first_name: Sonoko
full_name: Furuya, Sonoko
last_name: Furuya
- first_name: Kishio
full_name: Furuya, Kishio
last_name: Furuya
- first_name: Ryuichi
full_name: Ryuichi Shigemoto
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Masahiro
full_name: Sokabe, Masahiro
last_name: Sokabe
citation:
ama: Furuya S, Furuya K, Shigemoto R, Sokabe M. Localization of NK1 receptors and
roles of substance-P in subepithelial fibroblasts of rat intestinal villi. Cell
and Tissue Research. 2010;342(2):243-259. doi:10.1007/s00441-010-1056-7
apa: Furuya, S., Furuya, K., Shigemoto, R., & Sokabe, M. (2010). Localization
of NK1 receptors and roles of substance-P in subepithelial fibroblasts of rat
intestinal villi. Cell and Tissue Research. Springer. https://doi.org/10.1007/s00441-010-1056-7
chicago: Furuya, Sonoko, Kishio Furuya, Ryuichi Shigemoto, and Masahiro Sokabe.
“Localization of NK1 Receptors and Roles of Substance-P in Subepithelial Fibroblasts
of Rat Intestinal Villi.” Cell and Tissue Research. Springer, 2010. https://doi.org/10.1007/s00441-010-1056-7.
ieee: S. Furuya, K. Furuya, R. Shigemoto, and M. Sokabe, “Localization of NK1 receptors
and roles of substance-P in subepithelial fibroblasts of rat intestinal villi,”
Cell and Tissue Research, vol. 342, no. 2. Springer, pp. 243–259, 2010.
ista: Furuya S, Furuya K, Shigemoto R, Sokabe M. 2010. Localization of NK1 receptors
and roles of substance-P in subepithelial fibroblasts of rat intestinal villi.
Cell and Tissue Research. 342(2), 243–259.
mla: Furuya, Sonoko, et al. “Localization of NK1 Receptors and Roles of Substance-P
in Subepithelial Fibroblasts of Rat Intestinal Villi.” Cell and Tissue Research,
vol. 342, no. 2, Springer, 2010, pp. 243–59, doi:10.1007/s00441-010-1056-7.
short: S. Furuya, K. Furuya, R. Shigemoto, M. Sokabe, Cell and Tissue Research 342
(2010) 243–259.
date_created: 2018-12-11T11:58:04Z
date_published: 2010-11-01T00:00:00Z
date_updated: 2021-01-12T06:57:54Z
day: '01'
doi: 10.1007/s00441-010-1056-7
extern: 1
intvolume: ' 342'
issue: '2'
month: '11'
page: 243 - 259
publication: Cell and Tissue Research
publication_status: published
publisher: Springer
publist_id: '4395'
quality_controlled: 0
status: public
title: Localization of NK1 receptors and roles of substance-P in subepithelial fibroblasts
of rat intestinal villi
type: journal_article
volume: 342
year: '2010'
...
---
_id: '2507'
abstract:
- lang: eng
text: T-type calcium channels play a pivotal role in regulating neural membrane
excitability in the nervous system. However, the precise subcellular distributions
of T-type channel subunits and their implication for membrane excitability are
not well understood. Here we investigated the subcellular distribution of the
α1G subunit of the calcium channel which is expressed highly in the mouse dorsal
lateral geniculate nucleus (dLGN). Light microscopic analysis demonstrated that
dLGN exhibits intense immunoperoxidase reactivity for the α1G subunit. Electron
microscopic observation showed that the labeling was present in both the relay
cells and interneurons and was found in the somatodendritic, but not axonal, domains
of these cells. Most of the immunogold particles for the α1G subunit were either
associated with the plasma membrane or the intracellular membranes. Reconstruction
analysis of serial electron microscopic images revealed that the intensity of
the intracellular labeling exhibited a gradient such that the labeling density
was higher in the proximal dendrite and progressively decreased towards the distal
dendrite. In contrast, the plasma membrane-associated particles were distributed
with a uniform density over the somatodendritic surface of dLGN cells. The labeling
density in the relay cell plasma membrane was about 3-fold higher than that of
the interneurons. These results provide ultrastructural evidence for cell-type-specific
expression levels and for uniform expression density of the α1G subunit over the
plasma membrane of dLGN cells.
author:
- first_name: Laxmi
full_name: Parajuli, Laxmi K
last_name: Parajuli
- first_name: Yugo
full_name: Fukazawa, Yugo
last_name: Fukazawa
- first_name: Masahiko
full_name: Watanabe, Masahiko
last_name: Watanabe
- first_name: Ryuichi
full_name: Ryuichi Shigemoto
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
citation:
ama: Parajuli L, Fukazawa Y, Watanabe M, Shigemoto R. Subcellular distribution of
α1G subunit of T-type calcium channel in the mouse dorsal lateral geniculate nucleus.
Journal of Comparative Neurology. 2010;518(21):4362-4374. doi:10.1002/cne.22461
apa: Parajuli, L., Fukazawa, Y., Watanabe, M., & Shigemoto, R. (2010). Subcellular
distribution of α1G subunit of T-type calcium channel in the mouse dorsal lateral
geniculate nucleus. Journal of Comparative Neurology. Wiley-Blackwell.
https://doi.org/10.1002/cne.22461
chicago: Parajuli, Laxmi, Yugo Fukazawa, Masahiko Watanabe, and Ryuichi Shigemoto.
“Subcellular Distribution of Α1G Subunit of T-Type Calcium Channel in the Mouse
Dorsal Lateral Geniculate Nucleus.” Journal of Comparative Neurology. Wiley-Blackwell,
2010. https://doi.org/10.1002/cne.22461.
ieee: L. Parajuli, Y. Fukazawa, M. Watanabe, and R. Shigemoto, “Subcellular distribution
of α1G subunit of T-type calcium channel in the mouse dorsal lateral geniculate
nucleus,” Journal of Comparative Neurology, vol. 518, no. 21. Wiley-Blackwell,
pp. 4362–4374, 2010.
ista: Parajuli L, Fukazawa Y, Watanabe M, Shigemoto R. 2010. Subcellular distribution
of α1G subunit of T-type calcium channel in the mouse dorsal lateral geniculate
nucleus. Journal of Comparative Neurology. 518(21), 4362–4374.
mla: Parajuli, Laxmi, et al. “Subcellular Distribution of Α1G Subunit of T-Type
Calcium Channel in the Mouse Dorsal Lateral Geniculate Nucleus.” Journal of
Comparative Neurology, vol. 518, no. 21, Wiley-Blackwell, 2010, pp. 4362–74,
doi:10.1002/cne.22461.
short: L. Parajuli, Y. Fukazawa, M. Watanabe, R. Shigemoto, Journal of Comparative
Neurology 518 (2010) 4362–4374.
date_created: 2018-12-11T11:58:04Z
date_published: 2010-11-01T00:00:00Z
date_updated: 2021-01-12T06:57:54Z
day: '01'
doi: 10.1002/cne.22461
extern: 1
intvolume: ' 518'
issue: '21'
month: '11'
page: 4362 - 4374
publication: Journal of Comparative Neurology
publication_status: published
publisher: Wiley-Blackwell
publist_id: '4394'
quality_controlled: 0
status: public
title: Subcellular distribution of α1G subunit of T-type calcium channel in the mouse
dorsal lateral geniculate nucleus
type: journal_article
volume: 518
year: '2010'
...
---
_id: '2508'
abstract:
- lang: eng
text: 'The activity patterns of subthalamic nucleus (STN) neurons are intimately
linked to motor function and dysfunction and arise through the complex interaction
of intrinsic properties and inhibitory and excitatory synaptic inputs. In many
neurons, hyperpolarization-activated cyclic nucleotide-gated (HCN) channels play
key roles in intrinsic excitability and synaptic integration both under normal
conditions and in disease states. However, in STN neurons, which strongly express
HCN channels, their roles remain relatively obscure. To address this deficit,
complementary molecular and cellular electrophysiological, imaging, and computational
approaches were applied to the rat STN. Molecular profiling demonstrated that
individual STN neurons express mRNA encoding several HCN subunits, with HCN2 and
3 being the most abundant. Light and electron microscopic analysis showed that
HCN2 subunits are strongly expressed and distributed throughout the somatodendritic
plasma membrane. Voltage-, current-, and dynamic-clamp analysis, two-photon Ca
2+ imaging, and computational modeling revealed that HCN channels are activated
by GABA A receptor-mediated inputs and thus limit synaptic hyperpolarization and
deinactivation of low-voltage-activated Ca 2+ channels. Although HCN channels
also limited the temporal summation of EPSPs, generated through two-photon uncaging
of glutamate, this action was largely shunted by GABAergic inhibition that was
necessary for HCN channel activation. Together the data demonstrate that HCN channels
in STN neurons selectively counteract GABA A receptor-mediated inhibition arising
from the globus pallidus and thus promote single-spike activity rather than rebound
burst firing. '
author:
- first_name: Jeremy
full_name: Atherton, Jeremy F
last_name: Atherton
- first_name: Katsunori
full_name: Kitano, Katsunori
last_name: Kitano
- first_name: Jérôme
full_name: Baufreton, Jérôme
last_name: Baufreton
- first_name: Kai
full_name: Fan, Kai
last_name: Fan
- first_name: David
full_name: Wokosin, David L
last_name: Wokosin
- first_name: Tatiana
full_name: Tkatch, Tatiana
last_name: Tkatch
- first_name: Ryuichi
full_name: Ryuichi Shigemoto
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: James
full_name: Surmeier, James D
last_name: Surmeier
- first_name: Mark
full_name: Bevan, Mark D
last_name: Bevan
citation:
ama: Atherton J, Kitano K, Baufreton J, et al. Selective participation of somatodendritic
HCN channels in inhibitory but not excitatory synaptic integration in neurons
of the subthalamic nucleus. Journal of Neuroscience. 2010;30(47):16025-16040.
doi:10.1523/JNEUROSCI.3898-10.2010
apa: Atherton, J., Kitano, K., Baufreton, J., Fan, K., Wokosin, D., Tkatch, T.,
… Bevan, M. (2010). Selective participation of somatodendritic HCN channels in
inhibitory but not excitatory synaptic integration in neurons of the subthalamic
nucleus. Journal of Neuroscience. Society for Neuroscience. https://doi.org/10.1523/JNEUROSCI.3898-10.2010
chicago: Atherton, Jeremy, Katsunori Kitano, Jérôme Baufreton, Kai Fan, David Wokosin,
Tatiana Tkatch, Ryuichi Shigemoto, James Surmeier, and Mark Bevan. “Selective
Participation of Somatodendritic HCN Channels in Inhibitory but Not Excitatory
Synaptic Integration in Neurons of the Subthalamic Nucleus.” Journal of Neuroscience.
Society for Neuroscience, 2010. https://doi.org/10.1523/JNEUROSCI.3898-10.2010.
ieee: J. Atherton et al., “Selective participation of somatodendritic HCN
channels in inhibitory but not excitatory synaptic integration in neurons of the
subthalamic nucleus,” Journal of Neuroscience, vol. 30, no. 47. Society
for Neuroscience, pp. 16025–16040, 2010.
ista: Atherton J, Kitano K, Baufreton J, Fan K, Wokosin D, Tkatch T, Shigemoto R,
Surmeier J, Bevan M. 2010. Selective participation of somatodendritic HCN channels
in inhibitory but not excitatory synaptic integration in neurons of the subthalamic
nucleus. Journal of Neuroscience. 30(47), 16025–16040.
mla: Atherton, Jeremy, et al. “Selective Participation of Somatodendritic HCN Channels
in Inhibitory but Not Excitatory Synaptic Integration in Neurons of the Subthalamic
Nucleus.” Journal of Neuroscience, vol. 30, no. 47, Society for Neuroscience,
2010, pp. 16025–40, doi:10.1523/JNEUROSCI.3898-10.2010.
short: J. Atherton, K. Kitano, J. Baufreton, K. Fan, D. Wokosin, T. Tkatch, R. Shigemoto,
J. Surmeier, M. Bevan, Journal of Neuroscience 30 (2010) 16025–16040.
date_created: 2018-12-11T11:58:05Z
date_published: 2010-11-24T00:00:00Z
date_updated: 2021-01-12T06:57:54Z
day: '24'
doi: 10.1523/JNEUROSCI.3898-10.2010
extern: 1
intvolume: ' 30'
issue: '47'
month: '11'
page: 16025 - 16040
publication: Journal of Neuroscience
publication_status: published
publisher: Society for Neuroscience
publist_id: '4393'
quality_controlled: 0
status: public
title: Selective participation of somatodendritic HCN channels in inhibitory but not
excitatory synaptic integration in neurons of the subthalamic nucleus
type: journal_article
volume: 30
year: '2010'
...
---
_id: '2509'
abstract:
- lang: eng
text: Hippocampal CA1 pyramidal cells, which receive γ-aminobutyric acid (GABA)ergic
input from at least 18 types of presynaptic neuron, express 14 subunits of the
pentameric GABAA receptor. The relative contribution of any subunit to synaptic
and extrasynaptic receptors influences the dynamics of GABA and drug actions.
Synaptic receptors mediate phasic GABA-evoked conductance and extrasynaptic receptors
contribute to a tonic conductance. We used freeze-fracture replica-immunogold
labelling, a sensitive quantitative immunocytochemical method, to detect synaptic
and extrasynaptic pools of the alpha1, alpha2 and beta3 subunits. Antibodies to
the cytoplasmic loop of the subunits showed immunogold particles concentrated
on distinct clusters of intramembrane particles (IMPs) on the cytoplasmic face
of the plasma membrane on the somata, dendrites and axon initial segments, with
an abrupt decrease in labelling at the edge of the IMP cluster. Neuroligin-2,
a GABAergic synapse-specific adhesion molecule, co-labels all beta3 subunit-rich
IMP clusters, therefore we considered them synapses. Double-labelling for two
subunits showed that virtually all somatic synapses contain the alpha1, alpha2
and beta3 subunits. The extrasynaptic plasma membrane of the somata, dendrites
and dendritic spines showed low-density immunolabelling. Synaptic labelling densities
on somata for the alpha1, alpha2 and beta3 subunits were 78-132, 94 and 79 times
higher than on the extrasynaptic membranes, respectively. As GABAergic synapses
occupy 0.72% of the soma surface, the fraction of synaptic labelling was 33-48
(alpha1), 40 (alpha2) and 36 (beta3)% of the total somatic surface immunolabelling.
Assuming similar antibody access to all receptors, about 60% of these subunits
are in extrasynaptic receptors.
author:
- first_name: Yu
full_name: Kasugai, Yu
last_name: Kasugai
- first_name: Jerome
full_name: Swinny, Jerome D
last_name: Swinny
- first_name: John
full_name: Roberts, John D
last_name: Roberts
- first_name: Yannis
full_name: Dalezios, Yannis
last_name: Dalezios
- first_name: Yugo
full_name: Fukazawa, Yugo
last_name: Fukazawa
- first_name: Werner
full_name: Sieghart, Werner C
last_name: Sieghart
- first_name: Ryuichi
full_name: Ryuichi Shigemoto
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Péter
full_name: Somogyi, Péter
last_name: Somogyi
citation:
ama: Kasugai Y, Swinny J, Roberts J, et al. Quantitative localisation of synaptic
and extrasynaptic GABAA receptor subunits on hippocampal pyramidal cells by freeze-fracture
replica immunolabelling. European Journal of Neuroscience. 2010;32(11):1868-1888.
doi:10.1111/j.1460-9568.2010.07473.x
apa: Kasugai, Y., Swinny, J., Roberts, J., Dalezios, Y., Fukazawa, Y., Sieghart,
W., … Somogyi, P. (2010). Quantitative localisation of synaptic and extrasynaptic
GABAA receptor subunits on hippocampal pyramidal cells by freeze-fracture replica
immunolabelling. European Journal of Neuroscience. Wiley-Blackwell. https://doi.org/10.1111/j.1460-9568.2010.07473.x
chicago: Kasugai, Yu, Jerome Swinny, John Roberts, Yannis Dalezios, Yugo Fukazawa,
Werner Sieghart, Ryuichi Shigemoto, and Péter Somogyi. “Quantitative Localisation
of Synaptic and Extrasynaptic GABAA Receptor Subunits on Hippocampal Pyramidal
Cells by Freeze-Fracture Replica Immunolabelling.” European Journal of Neuroscience.
Wiley-Blackwell, 2010. https://doi.org/10.1111/j.1460-9568.2010.07473.x.
ieee: Y. Kasugai et al., “Quantitative localisation of synaptic and extrasynaptic
GABAA receptor subunits on hippocampal pyramidal cells by freeze-fracture replica
immunolabelling,” European Journal of Neuroscience, vol. 32, no. 11. Wiley-Blackwell,
pp. 1868–1888, 2010.
ista: Kasugai Y, Swinny J, Roberts J, Dalezios Y, Fukazawa Y, Sieghart W, Shigemoto
R, Somogyi P. 2010. Quantitative localisation of synaptic and extrasynaptic GABAA
receptor subunits on hippocampal pyramidal cells by freeze-fracture replica immunolabelling.
European Journal of Neuroscience. 32(11), 1868–1888.
mla: Kasugai, Yu, et al. “Quantitative Localisation of Synaptic and Extrasynaptic
GABAA Receptor Subunits on Hippocampal Pyramidal Cells by Freeze-Fracture Replica
Immunolabelling.” European Journal of Neuroscience, vol. 32, no. 11, Wiley-Blackwell,
2010, pp. 1868–88, doi:10.1111/j.1460-9568.2010.07473.x.
short: Y. Kasugai, J. Swinny, J. Roberts, Y. Dalezios, Y. Fukazawa, W. Sieghart,
R. Shigemoto, P. Somogyi, European Journal of Neuroscience 32 (2010) 1868–1888.
date_created: 2018-12-11T11:58:05Z
date_published: 2010-11-14T00:00:00Z
date_updated: 2021-01-12T06:57:55Z
day: '14'
doi: 10.1111/j.1460-9568.2010.07473.x
extern: 1
intvolume: ' 32'
issue: '11'
month: '11'
page: 1868 - 1888
publication: European Journal of Neuroscience
publication_status: published
publisher: Wiley-Blackwell
publist_id: '4392'
quality_controlled: 0
status: public
title: Quantitative localisation of synaptic and extrasynaptic GABAA receptor subunits
on hippocampal pyramidal cells by freeze-fracture replica immunolabelling
type: journal_article
volume: 32
year: '2010'
...
---
_id: '2510'
abstract:
- lang: eng
text: Neurons in the laterocapsular division of the central nucleus of the amygdala
(CeC), which is known as the "nociceptive amygdala," receive glutamatergic
inputs from the parabrachial nucleus (PB) and the basolateral nucleus of amygdala
(BLA), which convey nociceptive information from the dorsal horn of the spinal
cord and polymodal information from the thalamus and cortex, respectively. Here,
we examined the ultrastructural properties of PB- and BLA-CeC synapses identified
with EGFP-expressing lentivirus in rats. In addition, the density of synaptic
AMPA receptors (AMPARs) on CeC neurons was studied by using highly sensitive SDS-digested
freeze-fracture replica labeling (SDS-FRL). Afferents from the PB made asymmetrical
synapses mainly on dendritic shafts (88%), whereas those from the BLA were on
dendritic spines (81%). PB-CeC synapses in dendritic shafts were significantly
larger (median 0.072 μm 2) than BLA-CeC synapses in spines (median 0.058 μm 2;
P = 0.02). The dendritic shafts that made synapses with PB fibers were also significantly
larger than those that made synapses with BLA fibers, indicating that the PB fibers
make synapses on more proximal parts of dendrites than the BLA fibers. SDS-FRL
revealed that almost all excitatory postsynaptic sites have AMPARs in the CeC.
The density of AMPAR-specific gold particles in individual synapses was significantly
higher in spine synapses (median 510 particles/μm 2) than in shaft synapses (median
427 particles/μm 2; P = 0.01). These results suggest that distinct synaptic impacts
from PB- and BLA-CeC pathways contribute to the integration of nociceptive and
polymodal information in the CeC.
author:
- first_name: Yu
full_name: Dong, Yu-Lin
last_name: Dong
- first_name: Yugo
full_name: Fukazawa, Yugo
last_name: Fukazawa
- first_name: Wen
full_name: Wang, Wen
last_name: Wang
- first_name: Naomi
full_name: Kamasawa, Naomi
last_name: Kamasawa
- first_name: Ryuichi
full_name: Ryuichi Shigemoto
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
citation:
ama: Dong Y, Fukazawa Y, Wang W, Kamasawa N, Shigemoto R. Differential postsynaptic
compartments in the laterocapsular division of the central nucleus of amygdala
for afferents from the parabrachial nucleus and the basolateral nucleus in the
rat. Journal of Comparative Neurology. 2010;518(23):4771-4791. doi:10.1002/cne.22487
apa: Dong, Y., Fukazawa, Y., Wang, W., Kamasawa, N., & Shigemoto, R. (2010).
Differential postsynaptic compartments in the laterocapsular division of the central
nucleus of amygdala for afferents from the parabrachial nucleus and the basolateral
nucleus in the rat. Journal of Comparative Neurology. Wiley-Blackwell.
https://doi.org/10.1002/cne.22487
chicago: Dong, Yu, Yugo Fukazawa, Wen Wang, Naomi Kamasawa, and Ryuichi Shigemoto.
“Differential Postsynaptic Compartments in the Laterocapsular Division of the
Central Nucleus of Amygdala for Afferents from the Parabrachial Nucleus and the
Basolateral Nucleus in the Rat.” Journal of Comparative Neurology. Wiley-Blackwell,
2010. https://doi.org/10.1002/cne.22487.
ieee: Y. Dong, Y. Fukazawa, W. Wang, N. Kamasawa, and R. Shigemoto, “Differential
postsynaptic compartments in the laterocapsular division of the central nucleus
of amygdala for afferents from the parabrachial nucleus and the basolateral nucleus
in the rat,” Journal of Comparative Neurology, vol. 518, no. 23. Wiley-Blackwell,
pp. 4771–4791, 2010.
ista: Dong Y, Fukazawa Y, Wang W, Kamasawa N, Shigemoto R. 2010. Differential postsynaptic
compartments in the laterocapsular division of the central nucleus of amygdala
for afferents from the parabrachial nucleus and the basolateral nucleus in the
rat. Journal of Comparative Neurology. 518(23), 4771–4791.
mla: Dong, Yu, et al. “Differential Postsynaptic Compartments in the Laterocapsular
Division of the Central Nucleus of Amygdala for Afferents from the Parabrachial
Nucleus and the Basolateral Nucleus in the Rat.” Journal of Comparative Neurology,
vol. 518, no. 23, Wiley-Blackwell, 2010, pp. 4771–91, doi:10.1002/cne.22487.
short: Y. Dong, Y. Fukazawa, W. Wang, N. Kamasawa, R. Shigemoto, Journal of Comparative
Neurology 518 (2010) 4771–4791.
date_created: 2018-12-11T11:58:05Z
date_published: 2010-12-01T00:00:00Z
date_updated: 2021-01-12T06:57:55Z
day: '01'
doi: 10.1002/cne.22487
extern: 1
intvolume: ' 518'
issue: '23'
month: '12'
page: 4771 - 4791
publication: Journal of Comparative Neurology
publication_status: published
publisher: Wiley-Blackwell
publist_id: '4391'
quality_controlled: 0
status: public
title: Differential postsynaptic compartments in the laterocapsular division of the
central nucleus of amygdala for afferents from the parabrachial nucleus and the
basolateral nucleus in the rat
type: journal_article
volume: 518
year: '2010'
...