[{"publisher":"ACM","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","year":"2001","_id":"4449","article_processing_charge":"No","day":"26","date_updated":"2023-05-10T09:53:17Z","abstract":[{"lang":"eng","text":"Embedded software is software that interacts with physical processes. As em- bedded systems increasingly permeate our daily lives on all levels, from micros- copic devices to international networks, the cost-efficient development of reliable embedded software is one of the grand challenges in computer science today. The purpose of the workshop is to bring together researchers in all areas of computer science that are traditionally distinct but relevant to embedded software develop- ment, and to incubate a research community in this way. The workshop aims to cover all aspects of the design and implementation of embedded software, inclu- ding operating systems and middleware, programming languages and compilers, modeling and validation, software engineering and programming methodologies, scheduling and execution time analysis, networking and fault tolerance, as well as application areas, such as embedded control, real-time signal processing, and telecommunications."}],"type":"conference_editor","oa_version":"None","month":"09","title":"EMSOFT: Embedded Software","volume":2211,"date_created":"2018-12-11T12:08:54Z","publist_id":"283","alternative_title":["LNCS"],"editor":[{"last_name":"Henzinger","first_name":"Thomas A","full_name":"Henzinger, Thomas A","orcid":"0000-0002-2985-7724","id":"40876CD8-F248-11E8-B48F-1D18A9856A87"}],"status":"public","extern":"1","intvolume":" 2211","conference":{"location":"Tahoe City, CA, USA","name":"EMSOFT 2001: Embedded Software","start_date":"2001-10-08","end_date":"2001-10-10"},"language":[{"iso":"eng"}],"citation":{"apa":"Henzinger, T. A. (Ed.). (2001). EMSOFT: Embedded Software (Vol. 2211). Presented at the EMSOFT 2001: Embedded Software, Tahoe City, CA, USA: ACM. https://doi.org/10.1007/3-540-45449-7","mla":"Henzinger, Thomas A., editor. EMSOFT: Embedded Software. Vol. 2211, ACM, 2001, doi:10.1007/3-540-45449-7.","ista":"Henzinger TA ed. 2001. EMSOFT: Embedded Software, ACM,p.","ama":"Henzinger TA, ed. EMSOFT: Embedded Software. Vol 2211. ACM; 2001. doi:10.1007/3-540-45449-7","short":"T.A. Henzinger, ed., EMSOFT: Embedded Software, ACM, 2001.","chicago":"Henzinger, Thomas A, ed. EMSOFT: Embedded Software. Vol. 2211. ACM, 2001. https://doi.org/10.1007/3-540-45449-7.","ieee":"T. A. Henzinger, Ed., EMSOFT: Embedded Software, vol. 2211. ACM, 2001."},"publication_status":"published","publication_identifier":{"isbn":["9783540426738"]},"doi":"10.1007/3-540-45449-7","date_published":"2001-09-26T00:00:00Z","quality_controlled":"1"},{"volume":3,"title":"Some lessons from the HYTECH experience","date_created":"2018-12-11T12:09:02Z","publist_id":"253","author":[{"id":"40876CD8-F248-11E8-B48F-1D18A9856A87","orcid":"0000−0002−2985−7724","full_name":"Henzinger, Thomas A","first_name":"Thomas A","last_name":"Henzinger"},{"first_name":"Joerg","last_name":"Preussig","full_name":"Preussig, Joerg"},{"first_name":"Howard","last_name":"Wong Toi","full_name":"Wong Toi, Howard"}],"page":"2887 - 2892","type":"conference","month":"05","oa_version":"None","day":"01","date_updated":"2023-05-10T09:47:20Z","abstract":[{"text":"We provide an overview of the current status of HYTECH, and reflect on some of the lessons learned from our experiences with the tool. HYTECH is a symbolic model checker for mixed discrete-continuous systems that are modeled as automata with piecewise-constant polyhedral differential inclusions. The use of a formal input language and automated procedures for state-space traversal lay the foundation for formally verifying properties of hybrid dynamical systems. We describe some recent experiences analyzing three hybrid systems. We point out the successes and limitations of the tool. The analysis procedure has been extended in a number of ways to address some of the tool's shortcomings. We evaluate these extensions, and conclude with some desiderata for verification tools for hybrid systems.","lang":"eng"}],"_id":"4475","publication":"Proceedings of the 40th IEEE Conference on Decision and Control","article_processing_charge":"No","scopus_import":"1","publisher":"IEEE","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","year":"2001","doi":"10.1109/.2001.980714","date_published":"2001-05-01T00:00:00Z","quality_controlled":"1","publication_identifier":{"isbn":["0780370619"]},"publication_status":"published","conference":{"end_date":"2001-12-07","start_date":"2001-12-04","name":"CDC: Decision and Control","location":"Orlando, FL, USA"},"intvolume":" 3","extern":"1","language":[{"iso":"eng"}],"citation":{"short":"T.A. Henzinger, J. Preussig, H. Wong Toi, in:, Proceedings of the 40th IEEE Conference on Decision and Control, IEEE, 2001, pp. 2887–2892.","ieee":"T. A. Henzinger, J. Preussig, and H. Wong Toi, “Some lessons from the HYTECH experience,” in Proceedings of the 40th IEEE Conference on Decision and Control, Orlando, FL, USA, 2001, vol. 3, pp. 2887–2892.","chicago":"Henzinger, Thomas A, Joerg Preussig, and Howard Wong Toi. “Some Lessons from the HYTECH Experience.” In Proceedings of the 40th IEEE Conference on Decision and Control, 3:2887–92. IEEE, 2001. https://doi.org/10.1109/.2001.980714.","ista":"Henzinger TA, Preussig J, Wong Toi H. 2001. Some lessons from the HYTECH experience. Proceedings of the 40th IEEE Conference on Decision and Control. CDC: Decision and Control vol. 3, 2887–2892.","mla":"Henzinger, Thomas A., et al. “Some Lessons from the HYTECH Experience.” Proceedings of the 40th IEEE Conference on Decision and Control, vol. 3, IEEE, 2001, pp. 2887–92, doi:10.1109/.2001.980714.","apa":"Henzinger, T. A., Preussig, J., & Wong Toi, H. (2001). Some lessons from the HYTECH experience. In Proceedings of the 40th IEEE Conference on Decision and Control (Vol. 3, pp. 2887–2892). Orlando, FL, USA: IEEE. https://doi.org/10.1109/.2001.980714","ama":"Henzinger TA, Preussig J, Wong Toi H. Some lessons from the HYTECH experience. In: Proceedings of the 40th IEEE Conference on Decision and Control. Vol 3. IEEE; 2001:2887-2892. doi:10.1109/.2001.980714"},"status":"public"},{"language":[{"iso":"eng"}],"conference":{"end_date":"2001-03-30","location":"Rome, Italy","name":"HSCC: Hybrid Systems - Computation and Control","start_date":"2001-03-28"},"quality_controlled":"1","doi":"10.1007/3-540-45351-2_24","publication_identifier":{"isbn":["9783540418665"]},"article_processing_charge":"No","scopus_import":"1","publication":"Proceedings of the 4th International Workshop on Hybrid Systems","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","publisher":"Springer","publist_id":"250","title":"Assume-guarantee reasoning for hierarchical hybrid systems","day":"14","author":[{"last_name":"Henzinger","first_name":"Thomas A","full_name":"Henzinger, Thomas A","id":"40876CD8-F248-11E8-B48F-1D18A9856A87","orcid":"0000−0002−2985−7724"},{"full_name":"Minea, Marius","first_name":"Marius","last_name":"Minea"},{"first_name":"Vinayak","last_name":"Prabhu","full_name":"Prabhu, Vinayak"}],"citation":{"ama":"Henzinger TA, Minea M, Prabhu V. Assume-guarantee reasoning for hierarchical hybrid systems. In: Proceedings of the 4th International Workshop on Hybrid Systems. Vol 2034. Springer; 2001:275-290. doi:10.1007/3-540-45351-2_24","apa":"Henzinger, T. A., Minea, M., & Prabhu, V. (2001). Assume-guarantee reasoning for hierarchical hybrid systems. In Proceedings of the 4th International Workshop on Hybrid Systems (Vol. 2034, pp. 275–290). Rome, Italy: Springer. https://doi.org/10.1007/3-540-45351-2_24","mla":"Henzinger, Thomas A., et al. “Assume-Guarantee Reasoning for Hierarchical Hybrid Systems.” Proceedings of the 4th International Workshop on Hybrid Systems, vol. 2034, Springer, 2001, pp. 275–90, doi:10.1007/3-540-45351-2_24.","ista":"Henzinger TA, Minea M, Prabhu V. 2001. Assume-guarantee reasoning for hierarchical hybrid systems. Proceedings of the 4th International Workshop on Hybrid Systems. HSCC: Hybrid Systems - Computation and Control, LNCS, vol. 2034, 275–290.","chicago":"Henzinger, Thomas A, Marius Minea, and Vinayak Prabhu. “Assume-Guarantee Reasoning for Hierarchical Hybrid Systems.” In Proceedings of the 4th International Workshop on Hybrid Systems, 2034:275–90. Springer, 2001. https://doi.org/10.1007/3-540-45351-2_24.","ieee":"T. A. Henzinger, M. Minea, and V. Prabhu, “Assume-guarantee reasoning for hierarchical hybrid systems,” in Proceedings of the 4th International Workshop on Hybrid Systems, Rome, Italy, 2001, vol. 2034, pp. 275–290.","short":"T.A. Henzinger, M. Minea, V. Prabhu, in:, Proceedings of the 4th International Workshop on Hybrid Systems, Springer, 2001, pp. 275–290."},"extern":"1","intvolume":" 2034","status":"public","alternative_title":["LNCS"],"date_published":"2001-03-14T00:00:00Z","publication_status":"published","_id":"4477","year":"2001","acknowledgement":"Support for this research was provided in part by the AFOSR MURI grant F49620- 00-1-0327, and the DARPA SEC grant F33615-C-98-3614, the MARCO GSRC grant 98-DT-660, the NSF ITR grant CCR-0085949.","date_created":"2018-12-11T12:09:03Z","volume":2034,"abstract":[{"text":"The assume-guarantee paradigm is a powerful divide-and-conquer mechanism for decomposing a verification task about a system into subtasks about the individual components of the system. The key to assume-guarantee reasoning is to consider each component not in isolation, but in conjunction with assumptions about the context of the component. Assume-guarantee principles are known for purely concurrent contexts, which constrain the input data of a component, as well as for purely sequential contexts, which constrain the entry configurations of a component. We present a model for hierarchical system design which permits the arbitrary nesting of parallel as well as serial composition, and which supports an assume-guarantee principle for mixed parallel-serial contexts. Our model also supports both discrete and continuous processes, and is therefore well-suited for the modeling and analysis of embedded software systems which interact with real-world environments. Using an example of two cooperating robots, we show refinement between a high-level model which specifies continuous timing constraints and an implementation which relies on discrete sampling.","lang":"eng"}],"date_updated":"2023-05-09T14:47:37Z","type":"conference","oa_version":"None","month":"03","page":"275 - 290"},{"author":[{"full_name":"Henzinger, Thomas A","id":"40876CD8-F248-11E8-B48F-1D18A9856A87","orcid":"0000−0002−2985−7724","first_name":"Thomas A","last_name":"Henzinger"},{"full_name":"Horowitz, Benjamin","last_name":"Horowitz","first_name":"Benjamin"},{"first_name":"Christoph","last_name":"Kirsch","full_name":"Kirsch, Christoph"}],"page":"64 - 72","date_updated":"2023-05-10T09:37:20Z","day":"01","abstract":[{"lang":"eng","text":"Giotto is a principled, tool-supported design methodology for implementing embedded control systems on platforms of possibly distributed sensors, actuators, CPUs, and networks. Giotto is based on the principle that time-triggered task invocations plus time-triggered mode switches can form the abstract essence of programming real-time control systems. Giotto consists of a programming language with a formal semantics, and a retargetable compiler and runtime library. Giotto supports the automation of control system design by strictly separating platform-independent functionality and timing concerns from platform-dependent scheduling and communication issues. The time-triggered predictability of Giotto makes it particularly suitable for safety-critical applications with hard real-time constraints. We illustrate the platform-independence and time-triggered execution of Giotto by coordinating a heterogeneous flock of Intel x86 robots and Lego Mindstorms robots."}],"oa_version":"None","type":"conference","month":"06","title":"Embedded control systems development with Giotto","date_created":"2018-12-11T12:09:03Z","publist_id":"251","publisher":"ACM","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","acknowledgement":"We thank Rupak Majumdar for implementing a prototype Giotto compiler for Lego Mindstorms robots. We thank Dmitry Derevyanko and Winthrop Williams for building our Intel x86 robots. We thank Edward Lee and Xiaojun Liu for help with a Ptolemy II [4] implementation of Giotto. This research was supported in part by the DARPA SEC grant F33615-C-98-3614, the DARPA MoBIES grant F33615- 00-C-1703, the MARCO GSRC grant 98-DT-660, the AFOSR MURI grant F49620-00-1-0327, and the NSF ITR grant CCR-0085949.","year":"2001","publication":"Proceedings of the 2nd ACM SIGPLAN workshop on Languages, compilers and tools for embedded systems","_id":"4478","article_processing_charge":"No","scopus_import":"1","publication_status":"published","publication_identifier":{"isbn":["9781581134254"]},"date_published":"2001-06-01T00:00:00Z","doi":"10.1145/384197.384208","quality_controlled":"1","status":"public","extern":"1","conference":{"name":"LCTES: Languages, Compilers, and Tools for Embedded Systems","location":"New York, NY, United States"},"citation":{"apa":"Henzinger, T. A., Horowitz, B., & Kirsch, C. (2001). Embedded control systems development with Giotto. In Proceedings of the 2nd ACM SIGPLAN workshop on Languages, compilers and tools for embedded systems (pp. 64–72). New York, NY, United States: ACM. https://doi.org/10.1145/384197.384208","ista":"Henzinger TA, Horowitz B, Kirsch C. 2001. Embedded control systems development with Giotto. Proceedings of the 2nd ACM SIGPLAN workshop on Languages, compilers and tools for embedded systems. LCTES: Languages, Compilers, and Tools for Embedded Systems, 64–72.","mla":"Henzinger, Thomas A., et al. “Embedded Control Systems Development with Giotto.” Proceedings of the 2nd ACM SIGPLAN Workshop on Languages, Compilers and Tools for Embedded Systems, ACM, 2001, pp. 64–72, doi:10.1145/384197.384208.","ama":"Henzinger TA, Horowitz B, Kirsch C. Embedded control systems development with Giotto. In: Proceedings of the 2nd ACM SIGPLAN Workshop on Languages, Compilers and Tools for Embedded Systems. ACM; 2001:64-72. doi:10.1145/384197.384208","short":"T.A. Henzinger, B. Horowitz, C. Kirsch, in:, Proceedings of the 2nd ACM SIGPLAN Workshop on Languages, Compilers and Tools for Embedded Systems, ACM, 2001, pp. 64–72.","chicago":"Henzinger, Thomas A, Benjamin Horowitz, and Christoph Kirsch. “Embedded Control Systems Development with Giotto.” In Proceedings of the 2nd ACM SIGPLAN Workshop on Languages, Compilers and Tools for Embedded Systems, 64–72. ACM, 2001. https://doi.org/10.1145/384197.384208.","ieee":"T. A. Henzinger, B. Horowitz, and C. Kirsch, “Embedded control systems development with Giotto,” in Proceedings of the 2nd ACM SIGPLAN workshop on Languages, compilers and tools for embedded systems, New York, NY, United States, 2001, pp. 64–72."},"language":[{"iso":"eng"}]},{"acknowledgement":"This research was supported in part by the DARPA SEC grant F33615-C-98-3614 and by the MARCO GSRC grant 98-DT-660.","year":"2001","_id":"4479","page":"166 - 184","date_updated":"2023-05-10T09:42:10Z","abstract":[{"lang":"eng","text":"Giotto provides an abstract programmer’s model for the implementation of embedded control systems with hard real-time constraints. A typical control application consists of periodic software tasks together with a mode switching logic for enabling and disabling tasks. Giotto specifies time-triggered sensor readings, task invocations, and mode switches independent of any implementation platform. Giotto can be annotated with platform constraints such as task-to-host mappings, and task and communication schedules. The annotations are directives for the Giotto compiler, but they do not alter the functionality and timing of a Giotto program. By separating the platform-independent from the platform-dependent concerns, Giotto enables a great deal of flexibility in choosing control platforms as well as a great deal of automation in the validation and synthesis of control software. The time-triggered nature of Giotto achieves timing predictability, which makes Giotto particularly suitable for safety-critical applications."}],"type":"conference","oa_version":"None","month":"09","volume":2211,"date_created":"2018-12-11T12:09:04Z","alternative_title":["LNCS"],"status":"public","extern":"1","intvolume":" 2211","citation":{"ieee":"T. A. Henzinger, B. Horowitz, and C. Kirsch, “Giotto: A time-triggered language for embedded programming,” in Proceedings of the 1st International Workshop on Embedded Software, Tahoe City, CA, USA, 2001, vol. 2211, pp. 166–184.","chicago":"Henzinger, Thomas A, Benjamin Horowitz, and Christoph Kirsch. “Giotto: A Time-Triggered Language for Embedded Programming.” In Proceedings of the 1st International Workshop on Embedded Software, 2211:166–84. ACM, 2001. https://doi.org/10.1007/3-540-45449-7_12.","short":"T.A. Henzinger, B. Horowitz, C. Kirsch, in:, Proceedings of the 1st International Workshop on Embedded Software, ACM, 2001, pp. 166–184.","ama":"Henzinger TA, Horowitz B, Kirsch C. Giotto: A time-triggered language for embedded programming. In: Proceedings of the 1st International Workshop on Embedded Software. Vol 2211. ACM; 2001:166-184. doi:10.1007/3-540-45449-7_12","mla":"Henzinger, Thomas A., et al. “Giotto: A Time-Triggered Language for Embedded Programming.” Proceedings of the 1st International Workshop on Embedded Software, vol. 2211, ACM, 2001, pp. 166–84, doi:10.1007/3-540-45449-7_12.","ista":"Henzinger TA, Horowitz B, Kirsch C. 2001. Giotto: A time-triggered language for embedded programming. Proceedings of the 1st International Workshop on Embedded Software. EMSOFT: Embedded Software , LNCS, vol. 2211, 166–184.","apa":"Henzinger, T. A., Horowitz, B., & Kirsch, C. (2001). Giotto: A time-triggered language for embedded programming. In Proceedings of the 1st International Workshop on Embedded Software (Vol. 2211, pp. 166–184). Tahoe City, CA, USA: ACM. https://doi.org/10.1007/3-540-45449-7_12"},"publication_status":"published","date_published":"2001-09-26T00:00:00Z","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","publisher":"ACM","publication":"Proceedings of the 1st International Workshop on Embedded Software","article_processing_charge":"No","scopus_import":"1","author":[{"full_name":"Henzinger, Thomas A","id":"40876CD8-F248-11E8-B48F-1D18A9856A87","orcid":"0000−0002−2985−7724","first_name":"Thomas A","last_name":"Henzinger"},{"full_name":"Horowitz, Benjamin","first_name":"Benjamin","last_name":"Horowitz"},{"first_name":"Christoph","last_name":"Kirsch","full_name":"Kirsch, Christoph"}],"day":"26","title":"Giotto: A time-triggered language for embedded programming","publist_id":"252","conference":{"start_date":"2001-10-08","name":"EMSOFT: Embedded Software ","location":"Tahoe City, CA, USA","end_date":"2001-10-10"},"language":[{"iso":"eng"}],"publication_identifier":{"isbn":["9783540426738"]},"doi":"10.1007/3-540-45449-7_12","quality_controlled":"1"},{"volume":18,"title":"Chapter III Metabotropic glutamate receptors - immunocytochemical and in situ hybridization analyses","publist_id":"4407","date_created":"2018-12-11T11:58:00Z","author":[{"last_name":"Shigemoto","first_name":"Ryuichi","orcid":"0000-0001-8761-9444","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","full_name":"Shigemoto, Ryuichi"},{"full_name":"Mizuno, Noboru","last_name":"Mizuno","first_name":"Noboru"}],"page":"63 - 98","oa_version":"None","month":"01","type":"book_chapter","date_updated":"2023-05-03T12:27:40Z","day":"01","abstract":[{"text":"This chapter focuses on metabotropic glutamate receptors (mGluRs). These receptors are linked to several intracellular signal transduction mechanisms via G-protein and are implicated in diverse functions of the mammalian central nervous system (CNS). These functions include mediation of slow excitatory and inhibitory responses; regulation of calcium channels, potassium channels, and non-selective cation channels; inhibition and facilitation of transmitter release; induction of long-term potentiation and long-term depression; and regulation of neuronal development. Functional diversity of the metabotropic glutamate receptor is reflected in molecular diversity of receptor subtypes. The sites of action of mGluRs are widely found throughout different membrane compartments of neuronal and glial cells. The chapter reviews the differential subcellular localization of metabotropic glutamate receptors in relation to transmitter release sites. Patterns of subcellular localization of mGluRs are different among three subgroups: group I and III mGluRs are mainly localized to somatodendritic and axonal domains of neurons, respectively, while group II mGluRs are extensively localized to both domains as well as to glial cell processes.","lang":"eng"}],"_id":"2494","publication":"Glutamate","article_processing_charge":"No","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","publisher":"Elsevier","year":"2000","doi":"10.1016/S0924-8196(00)80044-5","date_published":"2000-01-01T00:00:00Z","quality_controlled":"1","publication_identifier":{"eissn":["0924-8196"]},"publication_status":"published","extern":"1","intvolume":" 18","citation":{"short":"R. Shigemoto, N. Mizuno, in:, Glutamate, Elsevier, 2000, pp. 63–98.","ieee":"R. Shigemoto and N. Mizuno, “Chapter III Metabotropic glutamate receptors - immunocytochemical and in situ hybridization analyses,” in Glutamate, vol. 18, Elsevier, 2000, pp. 63–98.","chicago":"Shigemoto, Ryuichi, and Noboru Mizuno. “Chapter III Metabotropic Glutamate Receptors - Immunocytochemical and in Situ Hybridization Analyses.” In Glutamate, 18:63–98. Elsevier, 2000. https://doi.org/10.1016/S0924-8196(00)80044-5.","mla":"Shigemoto, Ryuichi, and Noboru Mizuno. “Chapter III Metabotropic Glutamate Receptors - Immunocytochemical and in Situ Hybridization Analyses.” Glutamate, vol. 18, Elsevier, 2000, pp. 63–98, doi:10.1016/S0924-8196(00)80044-5.","ista":"Shigemoto R, Mizuno N. 2000.Chapter III Metabotropic glutamate receptors - immunocytochemical and in situ hybridization analyses. In: Glutamate. Handbook of Chemical Neuroanatomy, vol. 18, 63–98.","apa":"Shigemoto, R., & Mizuno, N. (2000). Chapter III Metabotropic glutamate receptors - immunocytochemical and in situ hybridization analyses. In Glutamate (Vol. 18, pp. 63–98). Elsevier. https://doi.org/10.1016/S0924-8196(00)80044-5","ama":"Shigemoto R, Mizuno N. Chapter III Metabotropic glutamate receptors - immunocytochemical and in situ hybridization analyses. In: Glutamate. Vol 18. Elsevier; 2000:63-98. doi:10.1016/S0924-8196(00)80044-5"},"language":[{"iso":"eng"}],"alternative_title":["Handbook of Chemical Neuroanatomy"],"status":"public"},{"issue":"1","language":[{"iso":"eng"}],"publication_identifier":{"issn":["0028-4793"]},"quality_controlled":"1","doi":"10.1056/NEJM200001063420104","pmid":1,"publisher":"Massachussetts Medical Society","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","article_type":"original","article_processing_charge":"No","scopus_import":"1","publication":"New England Journal of Medicine","day":"06","author":[{"full_name":"Sillevis Smitt, Peter","first_name":"Peter","last_name":"Sillevis Smitt"},{"last_name":"Kinoshita","first_name":"Ayae","full_name":"Kinoshita, Ayae"},{"last_name":"De Leeuw","first_name":"Bertie","full_name":"De Leeuw, Bertie"},{"full_name":"Moll, Wiebe","first_name":"Wiebe","last_name":"Moll"},{"full_name":"Coesmans, Michiel","first_name":"Michiel","last_name":"Coesmans"},{"last_name":"Jaarsma","first_name":"Dick","full_name":"Jaarsma, Dick"},{"full_name":"Henzen Logmans, Sonja","first_name":"Sonja","last_name":"Henzen Logmans"},{"last_name":"Vecht","first_name":"Charles","full_name":"Vecht, Charles"},{"full_name":"De Zeeuw, Chris","first_name":"Chris","last_name":"De Zeeuw"},{"first_name":"Naotaka","last_name":"Sekiyama","full_name":"Sekiyama, Naotaka"},{"last_name":"Nakanishi","first_name":"Shigetada","full_name":"Nakanishi, Shigetada"},{"full_name":"Shigemoto, Ryuichi","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8761-9444","first_name":"Ryuichi","last_name":"Shigemoto"}],"publist_id":"4300","title":"Paraneoplastic cerebellar ataxia due to autoantibodies against a glutamate receptor","external_id":{"pmid":["10620645"]},"status":"public","citation":{"short":"P. Sillevis Smitt, A. Kinoshita, B. De Leeuw, W. Moll, M. Coesmans, D. Jaarsma, S. Henzen Logmans, C. Vecht, C. De Zeeuw, N. Sekiyama, S. Nakanishi, R. Shigemoto, New England Journal of Medicine 342 (2000) 21–27.","chicago":"Sillevis Smitt, Peter, Ayae Kinoshita, Bertie De Leeuw, Wiebe Moll, Michiel Coesmans, Dick Jaarsma, Sonja Henzen Logmans, et al. “Paraneoplastic Cerebellar Ataxia Due to Autoantibodies against a Glutamate Receptor.” New England Journal of Medicine. Massachussetts Medical Society, 2000. https://doi.org/10.1056/NEJM200001063420104.","ieee":"P. Sillevis Smitt et al., “Paraneoplastic cerebellar ataxia due to autoantibodies against a glutamate receptor,” New England Journal of Medicine, vol. 342, no. 1. Massachussetts Medical Society, pp. 21–27, 2000.","apa":"Sillevis Smitt, P., Kinoshita, A., De Leeuw, B., Moll, W., Coesmans, M., Jaarsma, D., … Shigemoto, R. (2000). Paraneoplastic cerebellar ataxia due to autoantibodies against a glutamate receptor. New England Journal of Medicine. Massachussetts Medical Society. https://doi.org/10.1056/NEJM200001063420104","ista":"Sillevis Smitt P, Kinoshita A, De Leeuw B, Moll W, Coesmans M, Jaarsma D, Henzen Logmans S, Vecht C, De Zeeuw C, Sekiyama N, Nakanishi S, Shigemoto R. 2000. Paraneoplastic cerebellar ataxia due to autoantibodies against a glutamate receptor. New England Journal of Medicine. 342(1), 21–27.","mla":"Sillevis Smitt, Peter, et al. “Paraneoplastic Cerebellar Ataxia Due to Autoantibodies against a Glutamate Receptor.” New England Journal of Medicine, vol. 342, no. 1, Massachussetts Medical Society, 2000, pp. 21–27, doi:10.1056/NEJM200001063420104.","ama":"Sillevis Smitt P, Kinoshita A, De Leeuw B, et al. Paraneoplastic cerebellar ataxia due to autoantibodies against a glutamate receptor. New England Journal of Medicine. 2000;342(1):21-27. doi:10.1056/NEJM200001063420104"},"intvolume":" 342","extern":"1","oa":1,"publication_status":"published","main_file_link":[{"url":"https://www.nejm.org/doi/full/10.1056/nejm200001063420104","open_access":"1"}],"date_published":"2000-01-06T00:00:00Z","year":"2000","acknowledgement":"Supported in part by the Ministry of Education, Science, and Culture of Japan (Dr. Nakanishi and Dr. Shigemoto); CREST of Japan Science and Technology Corporation (Dr. Shigemoto); the Life Sciences Foundation (Dr. De Zeeuw); NWO (Dr. De Zeeuw and Dr. De Leeuw); and the Human Frontier Science Program (Dr. De Zeeuw and Dr. Shigemoto).\r\nDrs. Sillevis Smitt and Kinoshita contributed equally to the article. We are indebted to A. Aiba for providing mGluR1-deficient mice; to T. Maruyama for providing human mGluR1-expressing CHO cells; to H. Jingami for helpful discussion; to A. Uesugi for photographic assistance; to M. van den Bent and C. Gaillard for clinical information on the patients; and to J. van der Burg, S.K.E. Koekkoek, K.J. Reus, and C. Vermeer for technical assistance.","_id":"2598","type":"journal_article","month":"01","oa_version":"None","abstract":[{"lang":"eng","text":"There are many types of cerebellar ataxia, including ataxia due to congenital or metabolic disorders and a paraneoplastic form in patients with gynecologic cancer, breast cancer, lung cancer, or Hodgkin's disease.1 This paraneoplastic syndrome is the only type of cerebellar ataxia associated with autoantibodies against neuronal antigens. Often, the neuronal antigens are aberrantly expressed by the tumor cells.2-4 The antineuronal autoantibodies are believed to cause cerebellar ataxia, but this is unproved.5,6 In Hodgkin's disease, the lymphoma precedes the ataxia by months to years in 80 percent of patients, and ataxia often occurs during a prolonged complete remission.4 Among patients with this type of ataxia, 30 percent have anti–Purkinje-cell antibodies, some of which have the features of the neuronal antibody anti-Tr.4,7\r\n\r\nWe identified a new autoantibody in two patients with severe cerebellar ataxia that developed while they were in remission from Hodgkin's disease. The antibody reacts specifically with the metabotropic glutamate receptor mGluR1 in mouse brain. Metabotropic glutamate receptors belong to a large family of cell-surface receptors that transmit signals into the cell by coupling to guanine nucleotide-binding proteins (G proteins) in the cytoplasm. Purified IgG from the serum of both patients blocked the glutamate-stimulated formation of inositol phosphates in Chinese-hamster-ovary (CHO) cells that expressed mGluR1α, and the injection of IgG from serum or cerebrospinal fluid into the cerebellar subarachnoid space of mice caused severe, reversible ataxia. These results indicate that antineuronal autoantibodies can cause disease of the central nervous system by blocking neuronal receptors."}],"date_updated":"2023-05-03T11:14:19Z","page":"21 - 27","date_created":"2018-12-11T11:58:35Z","volume":342},{"doi":"10.1002/(SICI)1096-9861(20000306)418:2<156::AID-CNE3>3.0.CO;2-Z","quality_controlled":"1","publication_identifier":{"issn":["0021-9967"]},"language":[{"iso":"eng"}],"issue":"2","title":"Relationship between neurokinin-1 receptor and substance P in the striatum: Light and electron microscopic immunohistochemical study in the rat","publist_id":"4299","author":[{"full_name":"Li, Jin","last_name":"Li","first_name":"Jin"},{"first_name":"Dan","last_name":"Wang","full_name":"Wang, Dan"},{"full_name":"Kaneko, Takeshi","first_name":"Takeshi","last_name":"Kaneko"},{"last_name":"Shigemoto","first_name":"Ryuichi","orcid":"0000-0001-8761-9444","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","full_name":"Shigemoto, Ryuichi"},{"last_name":"Nomura","first_name":"Sakashi","full_name":"Nomura, Sakashi"},{"last_name":"Mizuno","first_name":"Noboru","full_name":"Mizuno, Noboru"}],"day":"06","publication":"Journal of Comparative Neurology","article_processing_charge":"No","scopus_import":"1","article_type":"original","publisher":"Wiley-Blackwell","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","pmid":1,"date_published":"2000-03-06T00:00:00Z","publication_status":"published","extern":"1","intvolume":" 418","citation":{"apa":"Li, J., Wang, D., Kaneko, T., Shigemoto, R., Nomura, S., & Mizuno, N. (2000). Relationship between neurokinin-1 receptor and substance P in the striatum: Light and electron microscopic immunohistochemical study in the rat. Journal of Comparative Neurology. Wiley-Blackwell. https://doi.org/10.1002/(SICI)1096-9861(20000306)418:2&lt;156::AID-CNE3&gt;3.0.CO;2-Z","ista":"Li J, Wang D, Kaneko T, Shigemoto R, Nomura S, Mizuno N. 2000. Relationship between neurokinin-1 receptor and substance P in the striatum: Light and electron microscopic immunohistochemical study in the rat. Journal of Comparative Neurology. 418(2), 156–163.","mla":"Li, Jin, et al. “Relationship between Neurokinin-1 Receptor and Substance P in the Striatum: Light and Electron Microscopic Immunohistochemical Study in the Rat.” Journal of Comparative Neurology, vol. 418, no. 2, Wiley-Blackwell, 2000, pp. 156–63, doi:10.1002/(SICI)1096-9861(20000306)418:2&lt;156::AID-CNE3&gt;3.0.CO;2-Z.","ama":"Li J, Wang D, Kaneko T, Shigemoto R, Nomura S, Mizuno N. Relationship between neurokinin-1 receptor and substance P in the striatum: Light and electron microscopic immunohistochemical study in the rat. Journal of Comparative Neurology. 2000;418(2):156-163. doi:10.1002/(SICI)1096-9861(20000306)418:2&lt;156::AID-CNE3&gt;3.0.CO;2-Z","short":"J. Li, D. Wang, T. Kaneko, R. Shigemoto, S. Nomura, N. Mizuno, Journal of Comparative Neurology 418 (2000) 156–163.","chicago":"Li, Jin, Dan Wang, Takeshi Kaneko, Ryuichi Shigemoto, Sakashi Nomura, and Noboru Mizuno. “Relationship between Neurokinin-1 Receptor and Substance P in the Striatum: Light and Electron Microscopic Immunohistochemical Study in the Rat.” Journal of Comparative Neurology. Wiley-Blackwell, 2000. https://doi.org/10.1002/(SICI)1096-9861(20000306)418:2&lt;156::AID-CNE3&gt;3.0.CO;2-Z.","ieee":"J. Li, D. Wang, T. Kaneko, R. Shigemoto, S. Nomura, and N. Mizuno, “Relationship between neurokinin-1 receptor and substance P in the striatum: Light and electron microscopic immunohistochemical study in the rat,” Journal of Comparative Neurology, vol. 418, no. 2. Wiley-Blackwell, pp. 156–163, 2000."},"status":"public","external_id":{"pmid":["10701441"]},"volume":418,"date_created":"2018-12-11T11:58:36Z","page":"156 - 163","abstract":[{"text":"The synaptic relationship between substance P (SP) and its receptor, i.e., neurokinin-1 receptor (NK1R), was examined in the striatum of the rat by confocal laser-scanning microscopy and electron microscopy. For confocal laser-scanning microscopy, triple-immunofluorescence histochemistry was performed to label NK1R, SP, and vesicular acetylcholine transporter (a specific marker for cholinergic neurons). In electron microscopic double- immunolabeling study, immunoreactivity for NK1R was detected with the silver- intensified gold method, while immunoreactivity for SP was detected with peroxidase immunohistochemistry. Simultaneous immunolabeling of NK1R and SP revealed significant mismatch at the synaptic level: although some SP- immunopositive axon terminals were in synaptic contact with NK1R- immunopositive sites of plasma membrane, NK1R-immunoreactivity was observed at both synaptic and non-synaptic sites of plasma membrane. Thus, SP released from the sites remote from NK1Rs might diffuse in the extracellular fluid to act, as a paracrine neurotransmitter, on NK1Rs distant from its releasing site. SP neurotransmission in the striatum might occur not only synaptically but also extrasynaptically. The SP-NK1R system might constitute an association system within the striatum.","lang":"eng"}],"date_updated":"2023-05-03T10:56:44Z","month":"03","type":"journal_article","oa_version":"None","_id":"2599","acknowledgement":"The authors are grateful for the photographic help of Ms. Yue-Ping Yuan, Mr. Akira Uesugi, Ms. Keiko Oka-moto, Mr. Nobuyuki Kobayashi, and Mr. Hideki Itabashi. The authors also express their gratitudes for the support of Dr. Kajitaro Morita in Morita Clinic of Internal Medicine and Pediatrics, Kadoma, Osaka, Japan","year":"2000"},{"publication_identifier":{"issn":["0168-0102"]},"doi":"10.1016/S0168-0102(00)00095-X","quality_controlled":"1","language":[{"iso":"eng"}],"issue":"4","author":[{"full_name":"Li, Jin","last_name":"Li","first_name":"Jin"},{"first_name":"Dan","last_name":"Wang","full_name":"Wang, Dan"},{"last_name":"Kaneko","first_name":"Takeshi","full_name":"Kaneko, Takeshi"},{"full_name":"Shigemoto, Ryuichi","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8761-9444","last_name":"Shigemoto","first_name":"Ryuichi"},{"full_name":"Nomura, Sakashi","last_name":"Nomura","first_name":"Sakashi"},{"full_name":"Mizuno, Noboru","last_name":"Mizuno","first_name":"Noboru"}],"day":"01","title":"The relationship between neurokinin-1 receptor and substance P in the medullary dorsal horn: A light and electron microscopic immunohistochemical study in the rat","publist_id":"4298","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","publisher":"Elsevier","pmid":1,"publication":"Neuroscience Research","article_processing_charge":"No","scopus_import":"1","article_type":"original","publication_status":"published","date_published":"2000-04-01T00:00:00Z","external_id":{"pmid":["10771111"]},"status":"public","extern":"1","intvolume":" 36","citation":{"ama":"Li J, Wang D, Kaneko T, Shigemoto R, Nomura S, Mizuno N. The relationship between neurokinin-1 receptor and substance P in the medullary dorsal horn: A light and electron microscopic immunohistochemical study in the rat. Neuroscience Research. 2000;36(4):327-334. doi:10.1016/S0168-0102(00)00095-X","apa":"Li, J., Wang, D., Kaneko, T., Shigemoto, R., Nomura, S., & Mizuno, N. (2000). The relationship between neurokinin-1 receptor and substance P in the medullary dorsal horn: A light and electron microscopic immunohistochemical study in the rat. Neuroscience Research. Elsevier. https://doi.org/10.1016/S0168-0102(00)00095-X","mla":"Li, Jin, et al. “The Relationship between Neurokinin-1 Receptor and Substance P in the Medullary Dorsal Horn: A Light and Electron Microscopic Immunohistochemical Study in the Rat.” Neuroscience Research, vol. 36, no. 4, Elsevier, 2000, pp. 327–34, doi:10.1016/S0168-0102(00)00095-X.","ista":"Li J, Wang D, Kaneko T, Shigemoto R, Nomura S, Mizuno N. 2000. The relationship between neurokinin-1 receptor and substance P in the medullary dorsal horn: A light and electron microscopic immunohistochemical study in the rat. Neuroscience Research. 36(4), 327–334.","chicago":"Li, Jin, Dan Wang, Takeshi Kaneko, Ryuichi Shigemoto, Sakashi Nomura, and Noboru Mizuno. “The Relationship between Neurokinin-1 Receptor and Substance P in the Medullary Dorsal Horn: A Light and Electron Microscopic Immunohistochemical Study in the Rat.” Neuroscience Research. Elsevier, 2000. https://doi.org/10.1016/S0168-0102(00)00095-X.","ieee":"J. Li, D. Wang, T. Kaneko, R. Shigemoto, S. Nomura, and N. Mizuno, “The relationship between neurokinin-1 receptor and substance P in the medullary dorsal horn: A light and electron microscopic immunohistochemical study in the rat,” Neuroscience Research, vol. 36, no. 4. Elsevier, pp. 327–334, 2000.","short":"J. Li, D. Wang, T. Kaneko, R. Shigemoto, S. Nomura, N. Mizuno, Neuroscience Research 36 (2000) 327–334."},"page":"327 - 334","date_updated":"2023-05-03T10:47:06Z","abstract":[{"lang":"eng","text":"The synaptic relationship between substance P (SP) and its receptor, i.e. neurokinin-1 receptor (NK1R), was examined in the superficial laminae of the caudal subnucleus of the spinal trigeminal nucleus (medullary dorsal horn; MDH) of the rat. For confocal laser-scanning microscopy, double-immunofluorescence histochemistry for NK1 and SP was performed. In electron microscopic double-immunolabeling study, immunoreactivity for NK1R was detected with the silver-intensified gold method, while immunoreactivity for SP was detected with peroxidase immunohistochemistry. SP-immunoreactive axon terminals were observed to be in synaptic (mostly asymmetric) contact with NK1R-immunoreactive neuronal profiles in lamina I and lamina IIo. Although some SP-immunoreactive axon terminals were in synaptic contact with NK1R-immunoreactive sites of plasma membranes, NK1R-immunoreactivity was observed at both synaptic and non-synaptic sites of plasma membrane. Thus, SP released from axon terminals might not only act on NK1Rs facing the SP-containing axon terminals, but also diffuse in the extracellular fluid for distances larger than the synaptic cleft to act on NK1Rs at some distances from the synaptic sites. "}],"type":"journal_article","month":"04","oa_version":"None","volume":36,"date_created":"2018-12-11T11:58:36Z","year":"2000","_id":"2600"},{"publication_identifier":{"issn":["0036-8075"]},"quality_controlled":"1","doi":"10.1126/science.288.5472.1832","language":[{"iso":"eng"}],"issue":"5472","day":"09","author":[{"full_name":"Ichise, Taeko","last_name":"Ichise","first_name":"Taeko"},{"first_name":"Masanobu","last_name":"Kano","full_name":"Kano, Masanobu"},{"full_name":"Hashimoto, Kouichi","first_name":"Kouichi","last_name":"Hashimoto"},{"last_name":"Yanagihara","first_name":"Dai","full_name":"Yanagihara, Dai"},{"full_name":"Nakao, Kazuki","last_name":"Nakao","first_name":"Kazuki"},{"first_name":"Ryuichi","last_name":"Shigemoto","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8761-9444","full_name":"Shigemoto, Ryuichi"},{"full_name":"Katsuki, Motoya","last_name":"Katsuki","first_name":"Motoya"},{"first_name":"Atsu","last_name":"Aiba","full_name":"Aiba, Atsu"}],"publist_id":"4297","title":"mGluR1 in cerebellar Purkinje cells essential for long-term depression, synapse elimination, and motor coordination","pmid":1,"publisher":"American Association for the Advancement of Science","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","scopus_import":"1","article_processing_charge":"No","article_type":"original","publication":"Science","publication_status":"published","date_published":"2000-06-09T00:00:00Z","external_id":{"pmid":["10846166 "]},"status":"public","citation":{"short":"T. Ichise, M. Kano, K. Hashimoto, D. Yanagihara, K. Nakao, R. Shigemoto, M. Katsuki, A. Aiba, Science 288 (2000) 1832–1835.","chicago":"Ichise, Taeko, Masanobu Kano, Kouichi Hashimoto, Dai Yanagihara, Kazuki Nakao, Ryuichi Shigemoto, Motoya Katsuki, and Atsu Aiba. “MGluR1 in Cerebellar Purkinje Cells Essential for Long-Term Depression, Synapse Elimination, and Motor Coordination.” Science. American Association for the Advancement of Science, 2000. https://doi.org/10.1126/science.288.5472.1832.","ieee":"T. Ichise et al., “mGluR1 in cerebellar Purkinje cells essential for long-term depression, synapse elimination, and motor coordination,” Science, vol. 288, no. 5472. American Association for the Advancement of Science, pp. 1832–1835, 2000.","apa":"Ichise, T., Kano, M., Hashimoto, K., Yanagihara, D., Nakao, K., Shigemoto, R., … Aiba, A. (2000). mGluR1 in cerebellar Purkinje cells essential for long-term depression, synapse elimination, and motor coordination. Science. American Association for the Advancement of Science. https://doi.org/10.1126/science.288.5472.1832","ista":"Ichise T, Kano M, Hashimoto K, Yanagihara D, Nakao K, Shigemoto R, Katsuki M, Aiba A. 2000. mGluR1 in cerebellar Purkinje cells essential for long-term depression, synapse elimination, and motor coordination. Science. 288(5472), 1832–1835.","mla":"Ichise, Taeko, et al. “MGluR1 in Cerebellar Purkinje Cells Essential for Long-Term Depression, Synapse Elimination, and Motor Coordination.” Science, vol. 288, no. 5472, American Association for the Advancement of Science, 2000, pp. 1832–35, doi:10.1126/science.288.5472.1832.","ama":"Ichise T, Kano M, Hashimoto K, et al. mGluR1 in cerebellar Purkinje cells essential for long-term depression, synapse elimination, and motor coordination. Science. 2000;288(5472):1832-1835. doi:10.1126/science.288.5472.1832"},"extern":"1","intvolume":" 288","date_updated":"2023-05-03T09:53:38Z","abstract":[{"lang":"eng","text":"Targeted deletion of metabotropic glutamate receptor-subtype 1 (mGluR1) gene can cause defects in development and function in the cerebellum. We introduced the mGluR1α transgene into mGluR1-null mutant [mGluR1 (-/-)] mice with a Purkinje cell (PC)-specific promoter. mGluR1-rescue mice showed normal cerebellar long-term depression and regression of multiple climbing fiber innervation, events significantly impaired in mGluR1 (-/-) mice. The impaired motor coordination was rescued by this transgene, in a dose-dependent manner. We propose that mGluR1 in PCs is a key molecule for normal synapse formation, synaptic plasticity, and motor control in the cerebellum."}],"oa_version":"None","month":"06","type":"journal_article","page":"1832 - 1835","date_created":"2018-12-11T11:58:36Z","volume":288,"year":"2000","_id":"2601"},{"year":"2000","acknowledgement":"This work was supported by Centre National de la Recherche Scientifique and grants from Association Française contre les Myopathies, Fondation pour la Recherche Médicale, Bayer (France), and Hoechst-Marrion-Roussel (FRHMR1/9702). We thank J. P. Pin and F. Ango for constructive discussion of this work. We also thank Dr. J. Saugstad (Atlanta, GA) for the rat mGluR7a cDNA, J. M. Sabatier (Marseille, France) for the synthesis of the 68 AA peptide, V. Homburger (Montpellier, France) for the anti-Gαo antibody, and B. Mouillac (Montpellier, France) for the anti-cMyc monoclonal antibody.","_id":"2602","type":"journal_article","oa_version":"Published Version","month":"11","date_updated":"2023-05-03T09:48:17Z","abstract":[{"text":"Although presynaptic localization of mGluR7 is well established, the mechanism by which the receptor may control Ca2+ channels in neurons is still unknown. We show here that cultured cerebellar granule cells express native metabotropic glutamate receptor type 7 (mGluR7) in neuritic processes, whereas transfected mGluR7 was also expressed in cell bodies. This allowed us to study the effect of the transfected receptor on somatic Ca2+ channels. In transfected neurons, mGuR7 selectively inhibited P/Q-type Ca2+ channels. The effect was mimicked by GTPγS and blocked by pertussis toxin (PTX) or a selective antibody raised against the G-protein αo subunit, indicating the involvement of a G(o)-like protein. The mGuR7 effect did not display the characteristics of a direct interaction between G-protein βγ subunits and the α1A Ca2+ channel subunit, but was abolished by quenching βγ subunits with specific intracellular peptides. Intracellular dialysis of G-protein βγ subunits did not mimic the action of mGluR7, suggesting that both G-protein βγ and αo subunits were required to mediate the effect. Inhibition of phospholipase C (PLC) blocked the inhibitory action of mGluR7, suggesting that a coincident activation of PLC by the G-protein βγ with αo subunits was required. The Ca2+ chelator BAPTA, as well as inhibition of either the inositol trisphosphate (IP3) receptor or protein kinase C (PKC) abolished the mGluR7 effect. Moreover, activation of native mGluR7 induced a PTX-dependent IP3 formation. These results indicated that IP3-mediated intracellular Ca2+ release was required for PKC-dependent inhibition of the Ca2+ channels. Possible control of synaptic transmission by the present mechanisms is discussed.","lang":"eng"}],"page":"7896 - 7904","date_created":"2018-12-11T11:58:37Z","volume":20,"status":"public","external_id":{"pmid":["11050109"]},"citation":{"ista":"Perroy J, Prezèau L, De Waard M, Shigemoto R, Bockaërt J, Fagni L. 2000. Selective blockade of P/Q-type calcium channels by the metabotropic glutamate receptor type 7 involves a phospholipase C pathway in neurons. Journal of Neuroscience. 20(21), 7896–7904.","mla":"Perroy, Julie, et al. “Selective Blockade of P/Q-Type Calcium Channels by the Metabotropic Glutamate Receptor Type 7 Involves a Phospholipase C Pathway in Neurons.” Journal of Neuroscience, vol. 20, no. 21, Society for Neuroscience, 2000, pp. 7896–904, doi:10.1523/JNEUROSCI.20-21-07896.2000.","apa":"Perroy, J., Prezèau, L., De Waard, M., Shigemoto, R., Bockaërt, J., & Fagni, L. (2000). Selective blockade of P/Q-type calcium channels by the metabotropic glutamate receptor type 7 involves a phospholipase C pathway in neurons. Journal of Neuroscience. Society for Neuroscience. https://doi.org/10.1523/JNEUROSCI.20-21-07896.2000","ama":"Perroy J, Prezèau L, De Waard M, Shigemoto R, Bockaërt J, Fagni L. Selective blockade of P/Q-type calcium channels by the metabotropic glutamate receptor type 7 involves a phospholipase C pathway in neurons. Journal of Neuroscience. 2000;20(21):7896-7904. doi:10.1523/JNEUROSCI.20-21-07896.2000","short":"J. Perroy, L. Prezèau, M. De Waard, R. Shigemoto, J. Bockaërt, L. Fagni, Journal of Neuroscience 20 (2000) 7896–7904.","ieee":"J. Perroy, L. Prezèau, M. De Waard, R. Shigemoto, J. Bockaërt, and L. Fagni, “Selective blockade of P/Q-type calcium channels by the metabotropic glutamate receptor type 7 involves a phospholipase C pathway in neurons,” Journal of Neuroscience, vol. 20, no. 21. Society for Neuroscience, pp. 7896–7904, 2000.","chicago":"Perroy, Julie, Laurent Prezèau, Michel De Waard, Ryuichi Shigemoto, Joël Bockaërt, and Laurent Fagni. “Selective Blockade of P/Q-Type Calcium Channels by the Metabotropic Glutamate Receptor Type 7 Involves a Phospholipase C Pathway in Neurons.” Journal of Neuroscience. Society for Neuroscience, 2000. https://doi.org/10.1523/JNEUROSCI.20-21-07896.2000."},"extern":"1","intvolume":" 20","publication_status":"published","oa":1,"main_file_link":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6772734/","open_access":"1"}],"date_published":"2000-11-01T00:00:00Z","pmid":1,"publisher":"Society for Neuroscience","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","article_type":"original","article_processing_charge":"No","scopus_import":"1","publication":"Journal of Neuroscience","day":"01","author":[{"first_name":"Julie","last_name":"Perroy","full_name":"Perroy, Julie"},{"full_name":"Prezèau, Laurent","first_name":"Laurent","last_name":"Prezèau"},{"full_name":"De Waard, Michel","last_name":"De Waard","first_name":"Michel"},{"last_name":"Shigemoto","first_name":"Ryuichi","orcid":"0000-0001-8761-9444","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","full_name":"Shigemoto, Ryuichi"},{"first_name":"Joël","last_name":"Bockaërt","full_name":"Bockaërt, Joël"},{"full_name":"Fagni, Laurent","last_name":"Fagni","first_name":"Laurent"}],"publist_id":"4296","title":"Selective blockade of P/Q-type calcium channels by the metabotropic glutamate receptor type 7 involves a phospholipase C pathway in neurons","issue":"21","language":[{"iso":"eng"}],"publication_identifier":{"issn":["0270-6474"]},"quality_controlled":"1","doi":"10.1523/JNEUROSCI.20-21-07896.2000"},{"author":[{"last_name":"Boudin","first_name":"Hélène","full_name":"Boudin, Hélène"},{"full_name":"Doan, Andrew","first_name":"Andrew","last_name":"Doan"},{"last_name":"Xia","first_name":"Jun","full_name":"Xia, Jun"},{"full_name":"Shigemoto, Ryuichi","orcid":"0000-0001-8761-9444","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","first_name":"Ryuichi","last_name":"Shigemoto"},{"full_name":"Huganir, Richard","first_name":"Richard","last_name":"Huganir"},{"last_name":"Worley","first_name":"Paul","full_name":"Worley, Paul"},{"first_name":"Ann","last_name":"Craig","full_name":"Craig, Ann"}],"day":"01","title":"Presynaptic clustering of mGluR7a requires the PICK1 PDZ domain binding site","publist_id":"4295","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","publisher":"Elsevier","pmid":1,"publication":"Neuron","scopus_import":"1","article_processing_charge":"No","article_type":"original","publication_identifier":{"issn":["0896-6273"]},"doi":"10.1016/S0896-6273(00)00127-6","quality_controlled":"1","language":[{"iso":"eng"}],"issue":"2","page":"485 - 497","abstract":[{"text":"Aggregation of neurotransmitter receptors at pre- and postsynaptic structures is crucial for efficient neuronal communication. In contrast to the wealth of information about postsynaptic specializations, little is known about the molecular organization of presynaptic membrane proteins. We show here that the metabotropic glutamate receptor mGluR7a, which localizes specifically to presynaptic active zones, interacts in vitro and in vivo with PICK1. Coexpression in heterologous systems induces coclustering dependent upon the extreme C terminus of mGluR7a and the PDZ domain of PICK1. mGluR7a and PICK1 localize to excitatory synapses in hippocampal neurons. Furthermore, whereas transfected mGluR7a clusters at presynaptic sites, mGluR7aΔ3 lacking the PICK1 binding site targets to axons but does not cluster. These results suggest that PICK1 is a component of the presynaptic machinery involved in mGlUR7a aggregation and in modulation of glutamate neurotransmission.","lang":"eng"}],"date_updated":"2023-05-03T09:41:55Z","oa_version":"None","type":"journal_article","month":"11","volume":28,"date_created":"2018-12-11T11:58:37Z","acknowledgement":"We thank Drs. S. Nakanishi, K. Moriyoshi, V. I. Gelfand, and P. J. Conn for gifts of cDNAs and antibodies, A. S. Serpinskaya and H. Wu for excellent preparation of neuron cultures, and H. J. Chung for help in immunoblots. This work was supported by the Pew Chari-table Trust and National Institutes of Health grants NS33184 and NS39286 (A. M. C.), K02MH01152 and NIDA DA10309 (P. W.), and a fellowship from IPSEN Foundation (H. B.). ","year":"2000","_id":"2603","publication_status":"published","date_published":"2000-11-01T00:00:00Z","status":"public","external_id":{"pmid":["11144358"]},"extern":"1","intvolume":" 28","citation":{"short":"H. Boudin, A. Doan, J. Xia, R. Shigemoto, R. Huganir, P. Worley, A. Craig, Neuron 28 (2000) 485–497.","chicago":"Boudin, Hélène, Andrew Doan, Jun Xia, Ryuichi Shigemoto, Richard Huganir, Paul Worley, and Ann Craig. “Presynaptic Clustering of MGluR7a Requires the PICK1 PDZ Domain Binding Site.” Neuron. Elsevier, 2000. https://doi.org/10.1016/S0896-6273(00)00127-6.","ieee":"H. Boudin et al., “Presynaptic clustering of mGluR7a requires the PICK1 PDZ domain binding site,” Neuron, vol. 28, no. 2. Elsevier, pp. 485–497, 2000.","apa":"Boudin, H., Doan, A., Xia, J., Shigemoto, R., Huganir, R., Worley, P., & Craig, A. (2000). Presynaptic clustering of mGluR7a requires the PICK1 PDZ domain binding site. Neuron. Elsevier. https://doi.org/10.1016/S0896-6273(00)00127-6","mla":"Boudin, Hélène, et al. “Presynaptic Clustering of MGluR7a Requires the PICK1 PDZ Domain Binding Site.” Neuron, vol. 28, no. 2, Elsevier, 2000, pp. 485–97, doi:10.1016/S0896-6273(00)00127-6.","ista":"Boudin H, Doan A, Xia J, Shigemoto R, Huganir R, Worley P, Craig A. 2000. Presynaptic clustering of mGluR7a requires the PICK1 PDZ domain binding site. Neuron. 28(2), 485–497.","ama":"Boudin H, Doan A, Xia J, et al. Presynaptic clustering of mGluR7a requires the PICK1 PDZ domain binding site. Neuron. 2000;28(2):485-497. doi:10.1016/S0896-6273(00)00127-6"}},{"publication_identifier":{"isbn":["9780821821572"]},"doi":"10.1090/amsip/016","quality_controlled":"1","language":[{"iso":"eng"}],"author":[{"last_name":"Erdös","first_name":"László","full_name":"Erdös, László","id":"4DBD5372-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5366-9603"}],"day":"01","arxiv":1,"title":"The kernel of Dirac operators on S3 and R3","publist_id":"4186","publisher":"American Mathematical Society","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","publication":"Differential Equations and Mathematical Physics","article_processing_charge":"No","publication_status":"published","date_published":"2000-01-01T00:00:00Z","alternative_title":["AMS/IP Studies in Advanced Mathematics"],"external_id":{"arxiv":["math-ph/0001036"]},"status":"public","extern":"1","intvolume":" 16","citation":{"short":"L. Erdös, in:, Differential Equations and Mathematical Physics, American Mathematical Society, 2000, pp. 111–119.","ieee":"L. Erdös, “The kernel of Dirac operators on S3 and R3,” in Differential Equations and Mathematical Physics, vol. 16, American Mathematical Society, 2000, pp. 111–119.","chicago":"Erdös, László. “The Kernel of Dirac Operators on S3 and R3.” In Differential Equations and Mathematical Physics, 16:111–19. American Mathematical Society, 2000. https://doi.org/10.1090/amsip/016.","ista":"Erdös L. 2000.The kernel of Dirac operators on S3 and R3. In: Differential Equations and Mathematical Physics. AMS/IP Studies in Advanced Mathematics, vol. 16, 111–119.","mla":"Erdös, László. “The Kernel of Dirac Operators on S3 and R3.” Differential Equations and Mathematical Physics, vol. 16, American Mathematical Society, 2000, pp. 111–19, doi:10.1090/amsip/016.","apa":"Erdös, L. (2000). The kernel of Dirac operators on S3 and R3. In Differential Equations and Mathematical Physics (Vol. 16, pp. 111–119). American Mathematical Society. https://doi.org/10.1090/amsip/016","ama":"Erdös L. The kernel of Dirac operators on S3 and R3. In: Differential Equations and Mathematical Physics. Vol 16. American Mathematical Society; 2000:111-119. doi:10.1090/amsip/016"},"page":"111 - 119","abstract":[{"text":"In this paper we describe an intrinsically geometric way of producing magnetic fields on §3 and $\\R^3$ for which the corresponding Dirac operators have a non-trivial kernel. In many cases we are able to compute the dimension of the kernel. In particular we can give examples where the kernel has any given dimension. This generalizes the examples of Loss and Yau (Commun. Math. Phys. 104 (1986) 283-290).","lang":"eng"}],"date_updated":"2023-05-03T09:37:03Z","type":"book_chapter","oa_version":"Preprint","month":"01","volume":16,"date_created":"2018-12-11T11:59:12Z","acknowledgement":"L. Erdös was supported by the N.S.F. grant DMS-9970323. J. P. Solovej was supported in parts by the EU TMR-grant FMRX-CT 96-0001 by MaPhySto — Centre for Mathematical Physics and Stochastics, funded by a grant from The Danish National Research Foundation and by a grant from the Danish Natural Science Research Council.","year":"2000","_id":"2710"},{"doi":"10.1002/(SICI)1097-0312(200006)53:6<667::AID-CPA1>3.0.CO;2-5","quality_controlled":"1","publication_identifier":{"issn":["0010-3640"]},"issue":"6","language":[{"iso":"eng"}],"arxiv":1,"title":"Linear Boltzmann equation as the weak coupling limit of a random Schrödinger equation","publist_id":"4161","author":[{"id":"4DBD5372-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5366-9603","full_name":"Erdös, László","first_name":"László","last_name":"Erdös"},{"full_name":"Yau, Horng","last_name":"Yau","first_name":"Horng"}],"day":"01","publication":"Communications on Pure and Applied Mathematics","article_type":"original","article_processing_charge":"No","scopus_import":"1","publisher":"Wiley-Blackwell","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","date_published":"2000-06-01T00:00:00Z","publication_status":"published","extern":"1","intvolume":" 53","citation":{"ista":"Erdös L, Yau H. 2000. Linear Boltzmann equation as the weak coupling limit of a random Schrödinger equation. Communications on Pure and Applied Mathematics. 53(6), 667–735.","mla":"Erdös, László, and Horng Yau. “Linear Boltzmann Equation as the Weak Coupling Limit of a Random Schrödinger Equation.” Communications on Pure and Applied Mathematics, vol. 53, no. 6, Wiley-Blackwell, 2000, pp. 667–735, doi:10.1002/(SICI)1097-0312(200006)53:6&lt;667::AID-CPA1&gt;3.0.CO;2-5.","apa":"Erdös, L., & Yau, H. (2000). Linear Boltzmann equation as the weak coupling limit of a random Schrödinger equation. Communications on Pure and Applied Mathematics. Wiley-Blackwell. https://doi.org/10.1002/(SICI)1097-0312(200006)53:6&lt;667::AID-CPA1&gt;3.0.CO;2-5","ama":"Erdös L, Yau H. Linear Boltzmann equation as the weak coupling limit of a random Schrödinger equation. Communications on Pure and Applied Mathematics. 2000;53(6):667-735. doi:10.1002/(SICI)1097-0312(200006)53:6&lt;667::AID-CPA1&gt;3.0.CO;2-5","short":"L. Erdös, H. Yau, Communications on Pure and Applied Mathematics 53 (2000) 667–735.","ieee":"L. Erdös and H. Yau, “Linear Boltzmann equation as the weak coupling limit of a random Schrödinger equation,” Communications on Pure and Applied Mathematics, vol. 53, no. 6. Wiley-Blackwell, pp. 667–735, 2000.","chicago":"Erdös, László, and Horng Yau. “Linear Boltzmann Equation as the Weak Coupling Limit of a Random Schrödinger Equation.” Communications on Pure and Applied Mathematics. Wiley-Blackwell, 2000. https://doi.org/10.1002/(SICI)1097-0312(200006)53:6&lt;667::AID-CPA1&gt;3.0.CO;2-5."},"external_id":{"arxiv":["math-ph/9901020"]},"status":"public","volume":53,"date_created":"2018-12-11T11:59:18Z","page":"667 - 735","month":"06","type":"journal_article","oa_version":"Preprint","abstract":[{"lang":"eng","text":"We study the time evolution of a quantum particle in a Gaussian random environment. We show that in the weak coupling limit the Wigner distribution of the wave function converges to a solution of a linear Boltzmann equation globally in time. The Boltzmann collision kernel is given by the Born approximation of the quantum differential scattering cross section."}],"date_updated":"2023-05-03T09:09:04Z","_id":"2731","acknowledgement":"Partially supported by U.S. National Science Foundation grants DMS-9403462, 9703752. We would like to thank H. Spohn for his several comments and discussions on this project. Part of this work was done during the time when L. E. visited the Erwin Schrödinger Institute in Vienna and when both authors visited the Center of Theoretical Sciences in Taiwan. We thank them for the hospitality and the support of this work.","year":"2000"},{"language":[{"iso":"eng"}],"issue":"3-4","doi":"10.1023/A:1018667323830","quality_controlled":"1","publication_identifier":{"issn":["0022-4715"]},"publication":"Journal of Statistical Physics","article_processing_charge":"No","scopus_import":"1","article_type":"original","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","publisher":"Springer","title":"Fokker-Planck equations as scaling limits of reversible quantum systems","publist_id":"4160","author":[{"full_name":"Castella, François","first_name":"François","last_name":"Castella"},{"last_name":"Erdös","first_name":"László","orcid":"0000-0001-5366-9603","id":"4DBD5372-F248-11E8-B48F-1D18A9856A87","full_name":"Erdös, László"},{"last_name":"Frommlet","first_name":"Florian","full_name":"Frommlet, Florian"},{"full_name":"Markowich, Peter","first_name":"Peter","last_name":"Markowich"}],"day":"01","extern":"1","intvolume":" 100","citation":{"short":"F. Castella, L. Erdös, F. Frommlet, P. Markowich, Journal of Statistical Physics 100 (2000) 543–601.","chicago":"Castella, François, László Erdös, Florian Frommlet, and Peter Markowich. “Fokker-Planck Equations as Scaling Limits of Reversible Quantum Systems.” Journal of Statistical Physics. Springer, 2000. https://doi.org/10.1023/A:1018667323830.","ieee":"F. Castella, L. Erdös, F. Frommlet, and P. Markowich, “Fokker-Planck equations as scaling limits of reversible quantum systems,” Journal of Statistical Physics, vol. 100, no. 3–4. Springer, pp. 543–601, 2000.","apa":"Castella, F., Erdös, L., Frommlet, F., & Markowich, P. (2000). Fokker-Planck equations as scaling limits of reversible quantum systems. Journal of Statistical Physics. Springer. https://doi.org/10.1023/A:1018667323830","mla":"Castella, François, et al. “Fokker-Planck Equations as Scaling Limits of Reversible Quantum Systems.” Journal of Statistical Physics, vol. 100, no. 3–4, Springer, 2000, pp. 543–601, doi:10.1023/A:1018667323830.","ista":"Castella F, Erdös L, Frommlet F, Markowich P. 2000. Fokker-Planck equations as scaling limits of reversible quantum systems. Journal of Statistical Physics. 100(3–4), 543–601.","ama":"Castella F, Erdös L, Frommlet F, Markowich P. Fokker-Planck equations as scaling limits of reversible quantum systems. Journal of Statistical Physics. 2000;100(3-4):543-601. doi:10.1023/A:1018667323830"},"status":"public","date_published":"2000-01-01T00:00:00Z","publication_status":"published","_id":"2732","acknowledgement":"The authors are indebted to H. Spohn for discussions. F.C. and L.E. were partially supported by the Erwin Schrödinger Institute in Vienna (Austria) during their visit, and they thank this institution for its hospitality. This work was supported by the TMR-Network ``Asymptotic Methods in Kinetic Theory'' number ERB FMBX CT97 0157 (F.C., F.F., and P.A.M.) and by NSF Grant DMS-9970323 (L.E.).","year":"2000","volume":100,"date_created":"2018-12-11T11:59:18Z","page":"543 - 601","date_updated":"2023-05-03T09:02:11Z","abstract":[{"text":"We consider a quantum particle moving in a harmonic exterior potential and linearly coupled to a heat bath of quantum oscillators. Caldeira and Leggett derived the Fokker Planck equation with friction for the Wigner distribution of the particle in the large-temperature limit: however, their (nonrigorous) derivation was not free of criticism, especially since the limiting equation is not of Lindblad form. In this paper we recover the correct form of their result in a rigorous way. We also point out that the source of the diffusion is physically restrictive under this scaling. We investigate the model at a fixed temperature and in the large-time limit, where the origin of the diffusion is a cumulative effect of many resonant collisions. We obtain a heat equation with a friction term for the radial process in phase space and we prove the Einstein relation in this case.","lang":"eng"}],"month":"01","type":"journal_article","oa_version":"None"},{"day":"01","author":[{"orcid":"0000-0001-5366-9603","id":"4DBD5372-F248-11E8-B48F-1D18A9856A87","full_name":"Erdös, László","last_name":"Erdös","first_name":"László"},{"full_name":"Loss, Michael","last_name":"Loss","first_name":"Michael"},{"full_name":"Vougalter, Vitali","first_name":"Vitali","last_name":"Vougalter"}],"publist_id":"4159","title":"Diamagnetic behavior of sums Dirichlet eigenvalues","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","publisher":"Association des Annales de l'Institut Fourier","article_processing_charge":"No","scopus_import":"1","article_type":"original","publication":"Annales de l'Institut Fourier","publication_identifier":{"issn":["0373-0956"]},"quality_controlled":"1","doi":"10.5802/aif.1777","language":[{"iso":"eng"}],"issue":"3","abstract":[{"lang":"eng","text":"The Li-Yau semiclassical lower bound for the sum of the first N eigenvalues of the Dirichlet–Laplacian is extended to Dirichlet–Laplacians with constant magnetic fields. Our method involves a new diamagnetic inequality for constant magnetic fields."}],"date_updated":"2023-05-03T08:56:17Z","type":"journal_article","month":"01","oa_version":"None","page":"891 - 907","date_created":"2018-12-11T11:59:19Z","volume":50,"year":"2000","_id":"2733","publication_status":"published","date_published":"2000-01-01T00:00:00Z","status":"public","citation":{"chicago":"Erdös, László, Michael Loss, and Vitali Vougalter. “Diamagnetic Behavior of Sums Dirichlet Eigenvalues.” Annales de l’Institut Fourier. Association des Annales de l’Institut Fourier, 2000. https://doi.org/10.5802/aif.1777.","ieee":"L. Erdös, M. Loss, and V. Vougalter, “Diamagnetic behavior of sums Dirichlet eigenvalues,” Annales de l’Institut Fourier, vol. 50, no. 3. Association des Annales de l’Institut Fourier, pp. 891–907, 2000.","short":"L. Erdös, M. Loss, V. Vougalter, Annales de l’Institut Fourier 50 (2000) 891–907.","ama":"Erdös L, Loss M, Vougalter V. Diamagnetic behavior of sums Dirichlet eigenvalues. Annales de l’Institut Fourier. 2000;50(3):891-907. doi:10.5802/aif.1777","apa":"Erdös, L., Loss, M., & Vougalter, V. (2000). Diamagnetic behavior of sums Dirichlet eigenvalues. Annales de l’Institut Fourier. Association des Annales de l’Institut Fourier. https://doi.org/10.5802/aif.1777","ista":"Erdös L, Loss M, Vougalter V. 2000. Diamagnetic behavior of sums Dirichlet eigenvalues. Annales de l’Institut Fourier. 50(3), 891–907.","mla":"Erdös, László, et al. “Diamagnetic Behavior of Sums Dirichlet Eigenvalues.” Annales de l’Institut Fourier, vol. 50, no. 3, Association des Annales de l’Institut Fourier, 2000, pp. 891–907, doi:10.5802/aif.1777."},"extern":"1","intvolume":" 50"},{"publist_id":"3546","title":"Interaction of Drosophila melanogaster prohormone convertase 2 and 7B2: Insect cell specific processing and secretion","day":"09","author":[{"first_name":"Jae","last_name":"Hwang","full_name":"Hwang, Jae"},{"last_name":"Siekhaus","first_name":"Daria E","id":"3D224B9E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8323-8353","full_name":"Siekhaus, Daria E"},{"full_name":"Fuller, Robert","first_name":"Robert","last_name":"Fuller"},{"full_name":"Taghert, Paul","first_name":"Paul","last_name":"Taghert"},{"first_name":"Iris","last_name":"Lindberg","full_name":"Lindberg, Iris"}],"scopus_import":"1","article_processing_charge":"No","article_type":"original","publication":"Journal of Biological Chemistry","pmid":1,"user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","publisher":"American Society for Biochemistry and Molecular Biology","quality_controlled":"1","doi":"10.1074/jbc.M000032200 ","publication_identifier":{"issn":["0021-9258"]},"language":[{"iso":"eng"}],"issue":"23","date_created":"2018-12-11T12:01:40Z","volume":275,"abstract":[{"lang":"eng","text":"The prohormone convertases (PCs) are an evolutionarily ancient group of proteases required for the maturation of neuropeptide and peptide hormone precursors. In Drosophila melanogaster, the homolog of prohormone convertase 2, dPC2 (amontillado), is required for normal hatching behavior, and immunoblotting data indicate that flies express 80- and 75-kDa forms of this protein. Because mouse PC2 (mPC2) requires 7B2, a helper protein for productive maturation, we searched the fly data base for the 7B2 signature motif PPNPCP and identified an expressed sequence tag clone encoding the entire open reading frame for this protein. dPC2 and d7B2 cDNAs were subcloned into expression vectors for transfection into HEK-293 cells; mPC2 and rat 7B2 were used as controls. Although active mPC2 was detected in medium in the presence of either d7B2 or r7B2, dPC2 showed no proteolytic activity upon coexpression of either d7B2 or r7B2. Labeling experiments showed that dPC2 was synthesized but not secreted from HEK-293 cells. However, when dPC2 and either d7B2 or r7B2 were coexpressed in Drosophila S2 cells, abundant immunoreactive dPC2 was secreted into the medium, coincident with the appearance of PC2 activity. Expression and secretion of dPC2 enzyme activity thus appears to require insect cell-specific posttranslational processing events. The significant differences in the cell biology of the insect and mammalian enzymes, with 7B2 absolutely required for secretion of dPC2 and zymogen conversion occurring intracellularly in the case of dPC2 but not mPC2, support the idea that the Drosophila enzyme has specific requirements for maturation and secretion that can be met only in insect cells."}],"date_updated":"2023-05-03T08:47:13Z","type":"journal_article","month":"06","oa_version":"Published Version","page":"17886 - 17893","_id":"3149","year":"2000","acknowledgement":"This work was supported by National Institutes of Health Grants DK49703 (to I. L.), NS21749 (to P. H. T.), and GM39697 (to R. S. F.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. 10749852. We thank members of the Lindberg laboratory and Laurent Muller for helpful comments, Bin Tu for construction of the C. elegans PC2 expression vector, and Joelle Finley for assistance with cell culture.","main_file_link":[{"url":"https://www.sciencedirect.com/science/article/pii/S0021925819833215?via%3Dihub","open_access":"1"}],"date_published":"2000-06-09T00:00:00Z","oa":1,"publication_status":"published","citation":{"ama":"Hwang J, Siekhaus DE, Fuller R, Taghert P, Lindberg I. Interaction of Drosophila melanogaster prohormone convertase 2 and 7B2: Insect cell specific processing and secretion. Journal of Biological Chemistry. 2000;275(23):17886-17893. doi:10.1074/jbc.M000032200 ","mla":"Hwang, Jae, et al. “Interaction of Drosophila Melanogaster Prohormone Convertase 2 and 7B2: Insect Cell Specific Processing and Secretion.” Journal of Biological Chemistry, vol. 275, no. 23, American Society for Biochemistry and Molecular Biology, 2000, pp. 17886–93, doi:10.1074/jbc.M000032200 .","ista":"Hwang J, Siekhaus DE, Fuller R, Taghert P, Lindberg I. 2000. Interaction of Drosophila melanogaster prohormone convertase 2 and 7B2: Insect cell specific processing and secretion. Journal of Biological Chemistry. 275(23), 17886–17893.","apa":"Hwang, J., Siekhaus, D. E., Fuller, R., Taghert, P., & Lindberg, I. (2000). Interaction of Drosophila melanogaster prohormone convertase 2 and 7B2: Insect cell specific processing and secretion. Journal of Biological Chemistry. American Society for Biochemistry and Molecular Biology. https://doi.org/10.1074/jbc.M000032200 ","ieee":"J. Hwang, D. E. Siekhaus, R. Fuller, P. Taghert, and I. Lindberg, “Interaction of Drosophila melanogaster prohormone convertase 2 and 7B2: Insect cell specific processing and secretion,” Journal of Biological Chemistry, vol. 275, no. 23. American Society for Biochemistry and Molecular Biology, pp. 17886–17893, 2000.","chicago":"Hwang, Jae, Daria E Siekhaus, Robert Fuller, Paul Taghert, and Iris Lindberg. “Interaction of Drosophila Melanogaster Prohormone Convertase 2 and 7B2: Insect Cell Specific Processing and Secretion.” Journal of Biological Chemistry. American Society for Biochemistry and Molecular Biology, 2000. https://doi.org/10.1074/jbc.M000032200 .","short":"J. Hwang, D.E. Siekhaus, R. Fuller, P. Taghert, I. Lindberg, Journal of Biological Chemistry 275 (2000) 17886–17893."},"extern":"1","intvolume":" 275","external_id":{"pmid":["10749852"]},"status":"public"},{"year":"2000","_id":"11126","abstract":[{"text":"Nuclear import of the two uracil-rich small nuclear ribonucleoprotein (U snRNP) components U1A and U2B′′ is mediated by unusually long and complex nuclear localization signals (NLSs). Here we investigate nuclear import of U1A and U2B′′ in vitro and demonstrate that it occurs by an active, saturable process. Several lines of evidence suggest that import of the two proteins occurs by an import mechanism different to those characterized previously. No cross competition is seen with a variety of previously studied NLSs. In contrast to import mediated by members of the importin-β family of nucleocytoplasmic transport receptors, U1A/U2B′′ import is not inhibited by either nonhydrolyzable guanosine triphosphate (GTP) analogues or by a mutant of the GTPase Ran that is incapable of GTP hydrolysis. Adenosine triphosphate is capable of supporting U1A and U2B′′ import, whereas neither nonhydrolyzable adenosine triphosphate analogues nor GTP can do so. U1A and U2B′′ import in vitro does not require the addition of soluble cytosolic proteins, but a factor or factors required for U1A and U2B′′ import remains tightly associated with the nuclear fraction of conventionally permeabilized cells. This activity can be solubilized in the presence of elevated MgCl2. These data suggest that U1A and U2B′′ import into the nucleus occurs by a hitherto uncharacterized mechanism.","lang":"eng"}],"date_updated":"2022-07-18T08:58:29Z","type":"journal_article","month":"01","oa_version":"None","page":"293-304","date_created":"2022-04-07T07:57:49Z","volume":148,"external_id":{"pmid":["10648562"]},"status":"public","citation":{"mla":"Hetzer, Martin, and Iain W. Mattaj. “An Atp-Dependent, Ran-Independent Mechanism for Nuclear Import of the U1a and U2b′′ Spliceosome Proteins.” Journal of Cell Biology, vol. 148, no. 2, Rockefeller University Press, 2000, pp. 293–304, doi:10.1083/jcb.148.2.293.","ista":"Hetzer M, Mattaj IW. 2000. An Atp-dependent, Ran-independent mechanism for nuclear import of the U1a and U2b′′ spliceosome proteins. Journal of Cell Biology. 148(2), 293–304.","apa":"Hetzer, M., & Mattaj, I. W. (2000). An Atp-dependent, Ran-independent mechanism for nuclear import of the U1a and U2b′′ spliceosome proteins. Journal of Cell Biology. Rockefeller University Press. https://doi.org/10.1083/jcb.148.2.293","ama":"Hetzer M, Mattaj IW. An Atp-dependent, Ran-independent mechanism for nuclear import of the U1a and U2b′′ spliceosome proteins. Journal of Cell Biology. 2000;148(2):293-304. doi:10.1083/jcb.148.2.293","short":"M. Hetzer, I.W. Mattaj, Journal of Cell Biology 148 (2000) 293–304.","ieee":"M. Hetzer and I. W. Mattaj, “An Atp-dependent, Ran-independent mechanism for nuclear import of the U1a and U2b′′ spliceosome proteins,” Journal of Cell Biology, vol. 148, no. 2. Rockefeller University Press, pp. 293–304, 2000.","chicago":"Hetzer, Martin, and Iain W. Mattaj. “An Atp-Dependent, Ran-Independent Mechanism for Nuclear Import of the U1a and U2b′′ Spliceosome Proteins.” Journal of Cell Biology. Rockefeller University Press, 2000. https://doi.org/10.1083/jcb.148.2.293."},"intvolume":" 148","extern":"1","publication_status":"published","date_published":"2000-01-24T00:00:00Z","pmid":1,"publisher":"Rockefeller University Press","user_id":"72615eeb-f1f3-11ec-aa25-d4573ddc34fd","scopus_import":"1","article_processing_charge":"No","article_type":"original","publication":"Journal of Cell Biology","day":"24","author":[{"last_name":"HETZER","first_name":"Martin W","full_name":"HETZER, Martin W","orcid":"0000-0002-2111-992X","id":"86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed"},{"last_name":"Mattaj","first_name":"Iain W.","full_name":"Mattaj, Iain W."}],"title":"An Atp-dependent, Ran-independent mechanism for nuclear import of the U1a and U2b′′ spliceosome proteins","language":[{"iso":"eng"}],"issue":"2","keyword":["Cell Biology"],"publication_identifier":{"eissn":["1540-8140"],"issn":["0021-9525"]},"quality_controlled":"1","doi":"10.1083/jcb.148.2.293"},{"oa":1,"publication_status":"published","date_published":"2000-06-01T00:00:00Z","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1016/S1097-2765(00)80266-X"}],"external_id":{"pmid":["10911995"]},"status":"public","intvolume":" 5","extern":"1","citation":{"chicago":"Hetzer, Martin, Daniel Bilbao-Cortés, Tobias C Walther, Oliver J Gruss, and Iain W Mattaj. “GTP Hydrolysis by Ran Is Required for Nuclear Envelope Assembly.” Molecular Cell. Elsevier, 2000. https://doi.org/10.1016/s1097-2765(00)80266-x.","ieee":"M. Hetzer, D. Bilbao-Cortés, T. C. Walther, O. J. Gruss, and I. W. Mattaj, “GTP hydrolysis by Ran is required for nuclear envelope assembly,” Molecular Cell, vol. 5, no. 6. Elsevier, pp. 1013–1024, 2000.","short":"M. Hetzer, D. Bilbao-Cortés, T.C. Walther, O.J. Gruss, I.W. Mattaj, Molecular Cell 5 (2000) 1013–1024.","ama":"Hetzer M, Bilbao-Cortés D, Walther TC, Gruss OJ, Mattaj IW. GTP hydrolysis by Ran is required for nuclear envelope assembly. Molecular Cell. 2000;5(6):1013-1024. doi:10.1016/s1097-2765(00)80266-x","apa":"Hetzer, M., Bilbao-Cortés, D., Walther, T. C., Gruss, O. J., & Mattaj, I. W. (2000). GTP hydrolysis by Ran is required for nuclear envelope assembly. Molecular Cell. Elsevier. https://doi.org/10.1016/s1097-2765(00)80266-x","ista":"Hetzer M, Bilbao-Cortés D, Walther TC, Gruss OJ, Mattaj IW. 2000. GTP hydrolysis by Ran is required for nuclear envelope assembly. Molecular Cell. 5(6), 1013–1024.","mla":"Hetzer, Martin, et al. “GTP Hydrolysis by Ran Is Required for Nuclear Envelope Assembly.” Molecular Cell, vol. 5, no. 6, Elsevier, 2000, pp. 1013–24, doi:10.1016/s1097-2765(00)80266-x."},"page":"1013-1024","date_updated":"2022-07-18T08:58:31Z","abstract":[{"text":"Nuclear formation in Xenopus egg extracts requires cytosol and is inhibited by GTPγS, indicating a requirement for GTPase activity. Nuclear envelope (NE) vesicle fusion is extensively inhibited by GTPγS and two mutant forms of the Ran GTPase, Q69L and T24N. Depletion of either Ran or RCC1, the exchange factor for Ran, from the assembly reaction also inhibits this step of NE formation. Ran depletion can be complemented by the addition of Ran loaded with either GTP or GDP but not with GTPγS. RCC1 depletion is only complemented by RCC1 itself or by RanGTP. Thus, generation of RanGTP by RCC1 and GTP hydrolysis by Ran are both required for the extensive membrane fusion events that lead to NE formation.","lang":"eng"}],"type":"journal_article","month":"06","oa_version":"Published Version","volume":5,"date_created":"2022-04-07T07:57:59Z","year":"2000","_id":"11127","publication_identifier":{"issn":["1097-2765"]},"doi":"10.1016/s1097-2765(00)80266-x","quality_controlled":"1","keyword":["Cell Biology","Molecular Biology"],"language":[{"iso":"eng"}],"issue":"6","author":[{"first_name":"Martin W","last_name":"HETZER","full_name":"HETZER, Martin W","id":"86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed","orcid":"0000-0002-2111-992X"},{"last_name":"Bilbao-Cortés","first_name":"Daniel","full_name":"Bilbao-Cortés, Daniel"},{"last_name":"Walther","first_name":"Tobias C","full_name":"Walther, Tobias C"},{"full_name":"Gruss, Oliver J","first_name":"Oliver J","last_name":"Gruss"},{"full_name":"Mattaj, Iain W","first_name":"Iain W","last_name":"Mattaj"}],"day":"01","title":"GTP hydrolysis by Ran is required for nuclear envelope assembly","user_id":"72615eeb-f1f3-11ec-aa25-d4573ddc34fd","publisher":"Elsevier","pmid":1,"publication":"Molecular Cell","article_processing_charge":"No","scopus_import":"1","article_type":"original"},{"day":"01","author":[{"last_name":"Henzinger","first_name":"Monika H","id":"540c9bbd-f2de-11ec-812d-d04a5be85630","orcid":"0000-0002-5008-6530","full_name":"Henzinger, Monika H"},{"full_name":"Rao, Satish","last_name":"Rao","first_name":"Satish"},{"full_name":"Gabow, Harold N.","last_name":"Gabow","first_name":"Harold N."}],"title":"Computing vertex connectivity: New bounds from old techniques","publisher":"Elsevier","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","article_type":"original","article_processing_charge":"No","scopus_import":"1","publication":"Journal of Algorithms","publication_identifier":{"issn":["0196-6774"]},"quality_controlled":"1","doi":"10.1006/jagm.1999.1055","issue":"2","language":[{"iso":"eng"}],"keyword":["Computational Theory and Mathematics","Computational Mathematics","Control and Optimization"],"oa_version":"None","type":"journal_article","month":"02","abstract":[{"text":"The vertex connectivity κ of a graph is the smallest number of vertices whose deletion separates the graph or makes it trivial. We present the fastest known deterministic algorithm for finding the vertex connectivity and a corresponding separator. The time for a digraph having n vertices and m edges is O(min{κ3 + n, κn}m); for an undirected graph the term m can be replaced by κn. A randomized algorithm finds κ with error probability 1/2 in time O(nm). If the vertices have nonnegative weights the weighted vertex connectivity is found in time O(κ1nmlog(n2/m)) where κ1 ≤ m/n is the unweighted vertex connectivity or in expected time O(nmlog(n2/m)) with error probability 1/2. The main algorithm combines two previous vertex connectivity algorithms and a generalization of the preflow-push algorithm of Hao and Orlin (1994, J. Algorithms17, 424–446) that computes edge connectivity.","lang":"eng"}],"date_updated":"2022-09-12T09:06:48Z","page":"222-250","date_created":"2022-07-28T08:56:10Z","volume":34,"year":"2000","_id":"11683","publication_status":"published","date_published":"2000-02-01T00:00:00Z","status":"public","citation":{"ama":"Henzinger MH, Rao S, Gabow HN. Computing vertex connectivity: New bounds from old techniques. Journal of Algorithms. 2000;34(2):222-250. doi:10.1006/jagm.1999.1055","apa":"Henzinger, M. H., Rao, S., & Gabow, H. N. (2000). Computing vertex connectivity: New bounds from old techniques. Journal of Algorithms. Elsevier. https://doi.org/10.1006/jagm.1999.1055","mla":"Henzinger, Monika H., et al. “Computing Vertex Connectivity: New Bounds from Old Techniques.” Journal of Algorithms, vol. 34, no. 2, Elsevier, 2000, pp. 222–50, doi:10.1006/jagm.1999.1055.","ista":"Henzinger MH, Rao S, Gabow HN. 2000. Computing vertex connectivity: New bounds from old techniques. Journal of Algorithms. 34(2), 222–250.","chicago":"Henzinger, Monika H, Satish Rao, and Harold N. Gabow. “Computing Vertex Connectivity: New Bounds from Old Techniques.” Journal of Algorithms. Elsevier, 2000. https://doi.org/10.1006/jagm.1999.1055.","ieee":"M. H. Henzinger, S. Rao, and H. N. Gabow, “Computing vertex connectivity: New bounds from old techniques,” Journal of Algorithms, vol. 34, no. 2. Elsevier, pp. 222–250, 2000.","short":"M.H. Henzinger, S. Rao, H.N. Gabow, Journal of Algorithms 34 (2000) 222–250."},"extern":"1","intvolume":" 34"}]