[{"author":[{"orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K","last_name":"Sixt","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K"},{"last_name":"Lämmermann","first_name":"Tim","full_name":"Lämmermann, Tim"}],"article_type":"original","date_updated":"2021-01-12T07:43:55Z","volume":769,"_id":"3505","date_created":"2018-12-11T12:03:41Z","citation":{"ama":"Sixt MK, Lämmermann T. In vitro analysis of chemotactic leukocyte migration in 3D environments. Cell Migration. 2011;769:149-165. doi:10.1007/978-1-61779-207-6_11","ista":"Sixt MK, Lämmermann T. 2011. In vitro analysis of chemotactic leukocyte migration in 3D environments. Cell Migration. 769, 149–165.","chicago":"Sixt, Michael K, and Tim Lämmermann. “In Vitro Analysis of Chemotactic Leukocyte Migration in 3D Environments.” Cell Migration. Springer, 2011. https://doi.org/10.1007/978-1-61779-207-6_11.","short":"M.K. Sixt, T. Lämmermann, Cell Migration 769 (2011) 149–165.","apa":"Sixt, M. K., & Lämmermann, T. (2011). In vitro analysis of chemotactic leukocyte migration in 3D environments. Cell Migration. Springer. https://doi.org/10.1007/978-1-61779-207-6_11","mla":"Sixt, Michael K., and Tim Lämmermann. “In Vitro Analysis of Chemotactic Leukocyte Migration in 3D Environments.” Cell Migration, vol. 769, Springer, 2011, pp. 149–65, doi:10.1007/978-1-61779-207-6_11.","ieee":"M. K. Sixt and T. Lämmermann, “In vitro analysis of chemotactic leukocyte migration in 3D environments,” Cell Migration, vol. 769. Springer, pp. 149–165, 2011."},"day":"17","intvolume":" 769","month":"05","department":[{"_id":"MiSi"}],"publisher":"Springer","quality_controlled":"1","page":"149 - 165","publication_status":"published","year":"2011","date_published":"2011-05-17T00:00:00Z","status":"public","oa_version":"Published Version","publist_id":"2882","oa":1,"article_processing_charge":"No","alternative_title":["Methods in Molecular Biology"],"language":[{"iso":"eng"}],"abstract":[{"text":"Cell migration on two-dimensional (2D) substrates follows entirely different rules than cell migration in three-dimensional (3D) environments. This is especially relevant for leukocytes that are able to migrate in the absence of adhesion receptors within the confined geometry of artificial 3D extracellular matrix scaffolds and within the interstitial space in vivo. Here, we describe in detail a simple and economical protocol to visualize dendritic cell migration in 3D collagen scaffolds along chemotactic gradients. This method can be adapted to other cell types and may serve as a physiologically relevant paradigm for the directed locomotion of most amoeboid cells.","lang":"eng"}],"publication":"Cell Migration","doi":"10.1007/978-1-61779-207-6_11","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","type":"journal_article","title":"In vitro analysis of chemotactic leukocyte migration in 3D environments","main_file_link":[{"open_access":"1","url":"https://pure.mpg.de/pubman/item/item_3219628_1/component/file_3219630/Sixt%20et%20al..pdf"}]},{"doi":"10.1007/978-1-61779-031-7_13","citation":{"mla":"Janovjak, Harald L., and Ehud Isacoff. “Structure-Based Design of Light-Controlled Proteins.” Photosensitive Molecules for the Control of Biological Function, vol. 55, Springer, 2011, pp. 233–66, doi:10.1007/978-1-61779-031-7_13.","apa":"Janovjak, H. L., & Isacoff, E. (2011). Structure-based design of light-controlled proteins. In Photosensitive Molecules for the Control of Biological Function (Vol. 55, pp. 233–266). Springer. https://doi.org/10.1007/978-1-61779-031-7_13","short":"H.L. Janovjak, E. Isacoff, in:, Photosensitive Molecules for the Control of Biological Function, Springer, 2011, pp. 233–266.","ista":"Janovjak HL, Isacoff E. 2011.Structure-based design of light-controlled proteins. In: Photosensitive Molecules for the Control of Biological Function. vol. 55, 233–266.","ama":"Janovjak HL, Isacoff E. Structure-based design of light-controlled proteins. In: Photosensitive Molecules for the Control of Biological Function. Vol 55. Springer; 2011:233-266. doi:10.1007/978-1-61779-031-7_13","chicago":"Janovjak, Harald L, and Ehud Isacoff. “Structure-Based Design of Light-Controlled Proteins.” In Photosensitive Molecules for the Control of Biological Function, 55:233–66. Springer, 2011. https://doi.org/10.1007/978-1-61779-031-7_13.","ieee":"H. L. Janovjak and E. Isacoff, “Structure-based design of light-controlled proteins,” in Photosensitive Molecules for the Control of Biological Function, vol. 55, Springer, 2011, pp. 233–266."},"publication":"Photosensitive Molecules for the Control of Biological Function","abstract":[{"text":"Small photochromic molecules are widespread in nature and serve as switches for a plethora of light-controlled processes. In a typical photoreceptor, the different geometries and polarities of the photochrome isomers are tightly coupled to functionally relevant conformational changes in the proteins. The past decade has seen extensive efforts to mimic nature and create proteins controlled by synthetic photochromes in the laboratory. Here, we discuss the role of molecular modeling to gain a structural understanding of photochromes and to design light-controlled peptides and proteins. We address several fundamental questions: What are the molecular structures of photochromes, particularly for metastable isomers that cannot be addressed experimentally? How are the structures of bistable photoisomers coupled to the conformational states of peptides and proteins? Can we design light-controlled proteins rapidly and reliably? After an introduction to the principles of molecular modeling, we answer these questions by examining systems that range from the size of isolated photochromes, to that of peptides and large cell surface receptors, each from its unique computational perspective.","lang":"eng"}],"month":"03","title":"Structure-based design of light-controlled proteins","intvolume":" 55","day":"16","type":"book_chapter","date_published":"2011-03-16T00:00:00Z","publication_status":"published","year":"2011","date_updated":"2021-01-12T07:51:45Z","page":"233 - 266","quality_controlled":0,"publisher":"Springer","author":[{"first_name":"Harald L","id":"33BA6C30-F248-11E8-B48F-1D18A9856A87","last_name":"Janovjak","full_name":"Harald Janovjak","orcid":"0000-0002-8023-9315"},{"last_name":"Isacoff","first_name":"Ehud","full_name":"Isacoff, Ehud Y"}],"date_created":"2018-12-11T12:04:49Z","_id":"3724","volume":55,"publist_id":"2504","extern":1,"status":"public"},{"article_processing_charge":"No","date_created":"2018-12-11T12:05:04Z","language":[{"iso":"eng"}],"_id":"3770","oa_version":"None","extern":"1","status":"public","volume":102,"publist_id":"2457","issue":"4","publication_status":"published","year":"2011","date_updated":"2021-01-12T07:52:05Z","date_published":"2011-04-01T00:00:00Z","author":[{"first_name":"Claudia","last_name":"Hollatz","full_name":"Hollatz, Claudia"},{"first_name":"Sibelle","last_name":"Vilaça","full_name":"Vilaça, Sibelle"},{"full_name":"Fernandes Redondo, Rodrigo A","orcid":"0000-0002-5837-2793","first_name":"Rodrigo A","id":"409D5C96-F248-11E8-B48F-1D18A9856A87","last_name":"Fernandes Redondo"},{"first_name":"Míriam","last_name":"Marmontel","full_name":"Marmontel, Míriam"},{"first_name":"Cyndi","last_name":"Baker","full_name":"Baker, Cyndi"},{"full_name":"Santos, Fabrício","first_name":"Fabrício","last_name":"Santos"}],"publisher":"Wiley","page":"812 - 827","month":"04","title":"The Amazon River system as an ecological barrier driving genetic differentiation of the pink dolphin (Inia geoffrensis)","intvolume":" 102","day":"01","type":"journal_article","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"apa":"Hollatz, C., Vilaça, S., Fernandes Redondo, R. A., Marmontel, M., Baker, C., & Santos, F. (2011). The Amazon River system as an ecological barrier driving genetic differentiation of the pink dolphin (Inia geoffrensis). Biological Journal of the Linnean Society. Wiley. https://doi.org/10.1111/j.1095-8312.2011.01616.x","short":"C. Hollatz, S. Vilaça, R.A. Fernandes Redondo, M. Marmontel, C. Baker, F. Santos, Biological Journal of the Linnean Society 102 (2011) 812–827.","mla":"Hollatz, Claudia, et al. “The Amazon River System as an Ecological Barrier Driving Genetic Differentiation of the Pink Dolphin (Inia Geoffrensis).” Biological Journal of the Linnean Society, vol. 102, no. 4, Wiley, 2011, pp. 812–27, doi:10.1111/j.1095-8312.2011.01616.x.","chicago":"Hollatz, Claudia, Sibelle Vilaça, Rodrigo A Fernandes Redondo, Míriam Marmontel, Cyndi Baker, and Fabrício Santos. “The Amazon River System as an Ecological Barrier Driving Genetic Differentiation of the Pink Dolphin (Inia Geoffrensis).” Biological Journal of the Linnean Society. Wiley, 2011. https://doi.org/10.1111/j.1095-8312.2011.01616.x.","ama":"Hollatz C, Vilaça S, Fernandes Redondo RA, Marmontel M, Baker C, Santos F. The Amazon River system as an ecological barrier driving genetic differentiation of the pink dolphin (Inia geoffrensis). Biological Journal of the Linnean Society. 2011;102(4):812-827. doi:10.1111/j.1095-8312.2011.01616.x","ista":"Hollatz C, Vilaça S, Fernandes Redondo RA, Marmontel M, Baker C, Santos F. 2011. The Amazon River system as an ecological barrier driving genetic differentiation of the pink dolphin (Inia geoffrensis). Biological Journal of the Linnean Society. 102(4), 812–827.","ieee":"C. Hollatz, S. Vilaça, R. A. Fernandes Redondo, M. Marmontel, C. Baker, and F. Santos, “The Amazon River system as an ecological barrier driving genetic differentiation of the pink dolphin (Inia geoffrensis),” Biological Journal of the Linnean Society, vol. 102, no. 4. Wiley, pp. 812–827, 2011."},"doi":"10.1111/j.1095-8312.2011.01616.x","abstract":[{"text":"The pink dolphin (Inia geoffrensis) is widely distributed along the Amazon and Orinoco basins, covering an area of approximately 7 million km2. Previous morphological and genetic studies have proposed the existence of at least two evolutionary significant units: one distributed across the Orinoco and Amazon basins and another confined to the Bolivian Amazon. The presence of barriers in the riverine environment has been suggested to play a significant role in shaping present-day patterns of ecological and genetic structure for this species. In the present study, we examined the phylogeographic structure, lineage divergence time and historical demography using mitochondrial (mt)DNA sequences in different pink dolphin populations distributed in large and small spatial scales, including two neighbouring Brazilian Amazon populations. mtDNA control region (CR) analysis revealed that the Brazilian haplotypes occupy an intermediate position compared to three previously studied geographic locations: the Colombian Amazon, the Colombian Orinoco, and the Bolivian Amazon. On a local scale, we have identified a pattern of maternal isolation between two neighbouring populations from Brazil. Six mtDNA CR haplotypes were identified in Brazil with no sharing between the two populations, as well as specific cytochrome b (cyt b) haplotypes identified in each locality. In addition, we analyzed autosomal microsatellites to investigate male-mediated gene flow and demographic changes within the study area in Brazil. Data analysis of 14 microsatellite loci failed to detect significant population subdivision, suggesting that male-mediated gene flow may maintain homogeneity between these two locations. Moreover, both mtDNA and microsatellite data indicate a major demographic collapse within Brazil in the late Pleistocene. Bayesian skyline plots (BSP) of mtDNA data revealed a stable population for Colombian and Brazilian Amazon lineages through time, whereas a population decline was demonstrated in the Colombian Orinoco lineage. Moreover, BSP and Tajima's D and Fu's Fs tests revealed a recent population expansion exclusively in the Bolivian sample. Finally, we estimated that the diversification of the Inia sp. lineage began in the Late Pliocene (approximately 3.1 Mya) and continued throughout the Pleistocene.","lang":"eng"}],"publication":"Biological Journal of the Linnean Society"},{"oa_version":"None","status":"public","volume":102,"issue":"3","publist_id":"2456","date_created":"2018-12-11T12:05:05Z","language":[{"iso":"eng"}],"_id":"3771","quality_controlled":"1","author":[{"first_name":"Ana","last_name":"Pavan","full_name":"Pavan, Ana"},{"first_name":"Felipe","last_name":"Martins","full_name":"Martins, Felipe"},{"full_name":"Santos, Fabrício","last_name":"Santos","first_name":"Fabrício"},{"full_name":"Ditchfield, Albert","last_name":"Ditchfield","first_name":"Albert"},{"last_name":"Fernandes Redondo","id":"409D5C96-F248-11E8-B48F-1D18A9856A87","first_name":"Rodrigo A","orcid":"0000-0002-5837-2793","full_name":"Fernandes Redondo, Rodrigo A"}],"publisher":"Wiley-Blackwell","page":"527 - 539","publication_status":"published","year":"2011","date_updated":"2021-01-12T07:52:05Z","date_published":"2011-02-10T00:00:00Z","intvolume":" 102","title":"Patterns of diversification in two species of short-tailed bats (Carollia Gray, 1838): the effects of historical fragmentation of Brazilian rainforests.","day":"10","type":"journal_article","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"FyKo"}],"month":"02","abstract":[{"text":"The small-sized frugivorous bat Carollia perspicillata is an understory specialist and occurs in a wide range of lowland habitats, tending to be more common in tropical dry or moist forests of South and Central America. Its sister species, Carollia brevicauda, occurs almost exclusively in the Amazon rainforest. A recent phylogeographic study proposed a hypothesis of origin and subsequent diversification for C. perspicillata along the Atlantic coastal forest of Brazil. Additionally, it also found two allopatric clades for C. brevicauda separated by the Amazon Basin. We used cytochrome b gene sequences and a more extensive sampling to test hypotheses related to the origin and diversification of C. perspicillata plus C. brevicauda clade in South America. The results obtained indicate that there are two sympatric evolutionary lineages within each species. In C. perspicillata, one lineage is limited to the Southern Atlantic Forest, whereas the other is widely distributed. Coalescent analysis points to a simultaneous origin for C. perspicillata and C. brevicauda, although no place for the diversification of each species can be firmly suggested. The phylogeographic pattern shown by C. perspicillata is also congruent with the Pleistocene refugia hypothesis as a likely vicariant phenomenon shaping the present distribution of its intraspecific lineages.","lang":"eng"}],"publication":"Biological Journal of the Linnean Society","citation":{"ista":"Pavan A, Martins F, Santos F, Ditchfield A, Fernandes Redondo RA. 2011. Patterns of diversification in two species of short-tailed bats (Carollia Gray, 1838): the effects of historical fragmentation of Brazilian rainforests. Biological Journal of the Linnean Society. 102(3), 527–539.","ama":"Pavan A, Martins F, Santos F, Ditchfield A, Fernandes Redondo RA. Patterns of diversification in two species of short-tailed bats (Carollia Gray, 1838): the effects of historical fragmentation of Brazilian rainforests. Biological Journal of the Linnean Society. 2011;102(3):527-539. doi:10.1111/j.1095-8312.2010.01601.x","chicago":"Pavan, Ana, Felipe Martins, Fabrício Santos, Albert Ditchfield, and Rodrigo A Fernandes Redondo. “Patterns of Diversification in Two Species of Short-Tailed Bats (Carollia Gray, 1838): The Effects of Historical Fragmentation of Brazilian Rainforests.” Biological Journal of the Linnean Society. Wiley-Blackwell, 2011. https://doi.org/10.1111/j.1095-8312.2010.01601.x.","mla":"Pavan, Ana, et al. “Patterns of Diversification in Two Species of Short-Tailed Bats (Carollia Gray, 1838): The Effects of Historical Fragmentation of Brazilian Rainforests.” Biological Journal of the Linnean Society, vol. 102, no. 3, Wiley-Blackwell, 2011, pp. 527–39, doi:10.1111/j.1095-8312.2010.01601.x.","apa":"Pavan, A., Martins, F., Santos, F., Ditchfield, A., & Fernandes Redondo, R. A. (2011). Patterns of diversification in two species of short-tailed bats (Carollia Gray, 1838): the effects of historical fragmentation of Brazilian rainforests. Biological Journal of the Linnean Society. Wiley-Blackwell. https://doi.org/10.1111/j.1095-8312.2010.01601.x","short":"A. Pavan, F. Martins, F. Santos, A. Ditchfield, R.A. Fernandes Redondo, Biological Journal of the Linnean Society 102 (2011) 527–539.","ieee":"A. Pavan, F. Martins, F. Santos, A. Ditchfield, and R. A. Fernandes Redondo, “Patterns of diversification in two species of short-tailed bats (Carollia Gray, 1838): the effects of historical fragmentation of Brazilian rainforests.,” Biological Journal of the Linnean Society, vol. 102, no. 3. Wiley-Blackwell, pp. 527–539, 2011."},"scopus_import":1,"doi":"10.1111/j.1095-8312.2010.01601.x"},{"pmid":1,"month":"02","department":[{"_id":"NiBa"}],"main_file_link":[{"open_access":"1","url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3183869/"}],"title":"Estimating linkage disequilibria","intvolume":" 106","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","day":"01","type":"journal_article","scopus_import":1,"doi":"10.1038/hdy.2010.67","citation":{"ama":"Barton NH. Estimating linkage disequilibria. Heredity. 2011;106(2):205-206. doi:10.1038/hdy.2010.67","ista":"Barton NH. 2011. Estimating linkage disequilibria. Heredity. 106(2), 205–206.","chicago":"Barton, Nicholas H. “Estimating Linkage Disequilibria.” Heredity. Nature Publishing Group, 2011. https://doi.org/10.1038/hdy.2010.67.","short":"N.H. Barton, Heredity 106 (2011) 205–206.","apa":"Barton, N. H. (2011). Estimating linkage disequilibria. Heredity. Nature Publishing Group. https://doi.org/10.1038/hdy.2010.67","mla":"Barton, Nicholas H. “Estimating Linkage Disequilibria.” Heredity, vol. 106, no. 2, Nature Publishing Group, 2011, pp. 205–06, doi:10.1038/hdy.2010.67.","ieee":"N. H. Barton, “Estimating linkage disequilibria,” Heredity, vol. 106, no. 2. Nature Publishing Group, pp. 205–206, 2011."},"publication":"Heredity","external_id":{"pmid":["20502479"]},"date_created":"2018-12-11T12:05:07Z","language":[{"iso":"eng"}],"_id":"3778","volume":106,"oa":1,"issue":"2","publist_id":"2449","oa_version":"Submitted Version","status":"public","date_published":"2011-02-01T00:00:00Z","year":"2011","publication_status":"published","date_updated":"2021-01-12T07:52:08Z","page":"205 - 206","publisher":"Nature Publishing Group","author":[{"full_name":"Barton, Nicholas H","orcid":"0000-0002-8548-5240","first_name":"Nicholas H","id":"4880FE40-F248-11E8-B48F-1D18A9856A87","last_name":"Barton"}]},{"date_published":"2011-01-01T00:00:00Z","year":"2011","publication_status":"published","date_updated":"2021-01-12T07:52:09Z","page":"359 - 367","quality_controlled":"1","author":[{"first_name":"Brittany Terese","id":"F65D502E-E68D-11E9-9252-C644099818F6","last_name":"Fasy","full_name":"Fasy, Brittany Terese"}],"publisher":"Szegedi Tudományegyetem","language":[{"iso":"eng"}],"date_created":"2018-12-11T12:05:08Z","_id":"3781","volume":77,"acknowledgement":"Funded by Graduate Aid in Areas of National Need (GAANN) Fellowship.","issue":"1-2","publist_id":"2446","oa_version":"None","status":"public","citation":{"ieee":"B. T. Fasy, “The difference in length of curves in R^n,” Acta Sci. Math. (Szeged), vol. 77, no. 1–2. Szegedi Tudományegyetem, pp. 359–367, 2011.","mla":"Fasy, Brittany Terese. “The Difference in Length of Curves in R^n.” Acta Sci. Math. (Szeged), vol. 77, no. 1–2, Szegedi Tudományegyetem, 2011, pp. 359–67.","apa":"Fasy, B. T. (2011). The difference in length of curves in R^n. Acta Sci. Math. (Szeged). Szegedi Tudományegyetem.","short":"B.T. Fasy, Acta Sci. Math. (Szeged) 77 (2011) 359–367.","chicago":"Fasy, Brittany Terese. “The Difference in Length of Curves in R^n.” Acta Sci. Math. (Szeged). Szegedi Tudományegyetem, 2011.","ama":"Fasy BT. The difference in length of curves in R^n. Acta Sci Math (Szeged). 2011;77(1-2):359-367.","ista":"Fasy BT. 2011. The difference in length of curves in R^n. Acta Sci. Math. (Szeged). 77(1–2), 359–367."},"publication":"Acta Sci. Math. (Szeged)","abstract":[{"lang":"eng","text":"We bound the difference in length of two curves in terms of their total curvatures and the Fréchet distance. The bound is independent of the dimension of the ambient Euclidean space, it improves upon a bound by Cohen-Steiner and Edelsbrunner, and it generalizes a result by Fáry and Chakerian."}],"department":[{"_id":"HeEd"}],"month":"01","intvolume":" 77","title":"The difference in length of curves in R^n","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","type":"journal_article","day":"01"},{"intvolume":" 91","day":"01","department":[{"_id":"NiBa"}],"month":"03","citation":{"ieee":"F. Palero, G. Guerao, P. Clark, and P. Abello, “Scyllarus arctus (Crustacea: Decapoda: Scyllaridae) final stage phyllosoma identified by DNA analysis, with morphological description,” Journal of the Marine Biological Association of the United Kingdom, vol. 91, no. 2. Cambridge University Press, pp. 485–492, 2011.","short":"F. Palero, G. Guerao, P. Clark, P. Abello, Journal of the Marine Biological Association of the United Kingdom 91 (2011) 485–492.","apa":"Palero, F., Guerao, G., Clark, P., & Abello, P. (2011). Scyllarus arctus (Crustacea: Decapoda: Scyllaridae) final stage phyllosoma identified by DNA analysis, with morphological description. Journal of the Marine Biological Association of the United Kingdom. Cambridge University Press. https://doi.org/10.1017/S0025315410000287","mla":"Palero, Ferran, et al. “Scyllarus Arctus (Crustacea: Decapoda: Scyllaridae) Final Stage Phyllosoma Identified by DNA Analysis, with Morphological Description.” Journal of the Marine Biological Association of the United Kingdom, vol. 91, no. 2, Cambridge University Press, 2011, pp. 485–92, doi:10.1017/S0025315410000287.","chicago":"Palero, Ferran, Guillermo Guerao, Paul Clark, and Pere Abello. “Scyllarus Arctus (Crustacea: Decapoda: Scyllaridae) Final Stage Phyllosoma Identified by DNA Analysis, with Morphological Description.” Journal of the Marine Biological Association of the United Kingdom. Cambridge University Press, 2011. https://doi.org/10.1017/S0025315410000287.","ista":"Palero F, Guerao G, Clark P, Abello P. 2011. Scyllarus arctus (Crustacea: Decapoda: Scyllaridae) final stage phyllosoma identified by DNA analysis, with morphological description. Journal of the Marine Biological Association of the United Kingdom. 91(2), 485–492.","ama":"Palero F, Guerao G, Clark P, Abello P. Scyllarus arctus (Crustacea: Decapoda: Scyllaridae) final stage phyllosoma identified by DNA analysis, with morphological description. Journal of the Marine Biological Association of the United Kingdom. 2011;91(2):485-492. doi:10.1017/S0025315410000287"},"scopus_import":1,"volume":91,"issue":"2","date_created":"2018-12-11T12:05:09Z","_id":"3784","author":[{"full_name":"Palero, Ferran","orcid":"0000-0002-0343-8329","id":"3F0E2A22-F248-11E8-B48F-1D18A9856A87","last_name":"Palero","first_name":"Ferran"},{"last_name":"Guerao","first_name":"Guillermo","full_name":"Guerao, Guillermo"},{"full_name":"Clark, Paul","first_name":"Paul","last_name":"Clark"},{"first_name":"Pere","last_name":"Abello","full_name":"Abello, Pere"}],"article_type":"original","date_updated":"2021-01-12T07:52:10Z","title":"Scyllarus arctus (Crustacea: Decapoda: Scyllaridae) final stage phyllosoma identified by DNA analysis, with morphological description","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","type":"journal_article","main_file_link":[{"url":"https://digital.csic.es/bitstream/10261/32783/3/Palero_et_al_2011.pdf","open_access":"1"}],"abstract":[{"lang":"eng","text":"Advanced stages of Scyllarus phyllosoma larvae were collected by demersal trawling during fishery research surveys in the western Mediterranean Sea in 2003–2005. Nucleotide sequence analysis of the mitochondrial 16S rDNA gene allowed the final-stage phyllosoma of Scyllarus arctus to be identified among these larvae. Its morphology is described and illustrated. This constitutes the second complete description of a Scyllaridae phyllosoma with its specific identity being validated by molecular techniques (the first was S. pygmaeus). These results also solved a long lasting taxonomic anomaly of several species assigned to the ancient genus Phyllosoma Leach, 1814. Detailed examination indicated that the final-stage phyllosoma of S. arctus shows closer affinities with the American scyllarid Scyllarus depressus or with the Australian Scyllarus sp. b (sensu Phillips et al., 1981) than to its sympatric species S. pygmaeus."}],"publication":"Journal of the Marine Biological Association of the United Kingdom","doi":"10.1017/S0025315410000287","oa_version":"Published Version","status":"public","publist_id":"2443","oa":1,"article_processing_charge":"No","language":[{"iso":"eng"}],"quality_controlled":"1","publisher":"Cambridge University Press","page":"485 - 492","year":"2011","publication_status":"published","date_published":"2011-03-01T00:00:00Z"},{"intvolume":" 95","title":"Cell sorting in development","day":"01","type":"book_chapter","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"CaHe"}],"month":"01","publication":"Forces and Tension in Development","abstract":[{"lang":"eng","text":"During the development of multicellular organisms, cell fate specification is followed by the sorting of different cell types into distinct domains from where the different tissues and organs are formed. Cell sorting involves both the segregation of a mixed population of cells with different fates and properties into distinct domains, and the active maintenance of their segregated state. Because of its biological importance and apparent resemblance to fluid segregation in physics, cell sorting was extensively studied by both biologists and physicists over the last decades. Different theories were developed that try to explain cell sorting on the basis of the physical properties of the constituent cells. However, only recently the molecular and cellular mechanisms that control the physical properties driving cell sorting, have begun to be unraveled. In this review, we will provide an overview of different cell-sorting processes in development and discuss how these processes can be explained by the different sorting theories, and how these theories in turn can be connected to the molecular and cellular mechanisms driving these processes."}],"scopus_import":"1","doi":"10.1016/B978-0-12-385065-2.00006-2","editor":[{"first_name":"Michel","last_name":"Labouesse","full_name":"Labouesse, Michel"}],"citation":{"short":"G. Krens, C.-P.J. Heisenberg, in:, M. Labouesse (Ed.), Forces and Tension in Development, Elsevier, 2011, pp. 189–213.","apa":"Krens, G., & Heisenberg, C.-P. J. (2011). Cell sorting in development. In M. Labouesse (Ed.), Forces and Tension in Development (Vol. 95, pp. 189–213). Elsevier. https://doi.org/10.1016/B978-0-12-385065-2.00006-2","mla":"Krens, Gabriel, and Carl-Philipp J. Heisenberg. “Cell Sorting in Development.” Forces and Tension in Development, edited by Michel Labouesse, vol. 95, Elsevier, 2011, pp. 189–213, doi:10.1016/B978-0-12-385065-2.00006-2.","ista":"Krens G, Heisenberg C-PJ. 2011.Cell sorting in development. In: Forces and Tension in Development. Current Topics in Developmental Biology, vol. 95, 189–213.","chicago":"Krens, Gabriel, and Carl-Philipp J Heisenberg. “Cell Sorting in Development.” In Forces and Tension in Development, edited by Michel Labouesse, 95:189–213. Elsevier, 2011. https://doi.org/10.1016/B978-0-12-385065-2.00006-2.","ama":"Krens G, Heisenberg C-PJ. Cell sorting in development. In: Labouesse M, ed. Forces and Tension in Development. Vol 95. Elsevier; 2011:189-213. doi:10.1016/B978-0-12-385065-2.00006-2","ieee":"G. Krens and C.-P. J. Heisenberg, “Cell sorting in development,” in Forces and Tension in Development, vol. 95, M. Labouesse, Ed. Elsevier, 2011, pp. 189–213."},"volume":95,"publist_id":"2436","oa_version":"None","status":"public","date_created":"2018-12-11T12:05:11Z","alternative_title":["Current Topics in Developmental Biology"],"language":[{"iso":"eng"}],"_id":"3791","article_processing_charge":"No","page":"189 - 213","quality_controlled":"1","publisher":"Elsevier","author":[{"orcid":"0000-0003-4761-5996","full_name":"Krens, Gabriel","first_name":"Gabriel","last_name":"Krens","id":"2B819732-F248-11E8-B48F-1D18A9856A87"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","last_name":"Heisenberg","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566"}],"date_published":"2011-01-01T00:00:00Z","publication_status":"published","year":"2011","date_updated":"2021-01-12T07:52:13Z"},{"doi":"10.1007/978-3-642-19391-0_2","abstract":[{"text":"We address the problem of covering ℝ n with congruent balls, while minimizing the number of balls that contain an average point. Considering the 1-parameter family of lattices defined by stretching or compressing the integer grid in diagonal direction, we give a closed formula for the covering density that depends on the distortion parameter. We observe that our family contains the thinnest lattice coverings in dimensions 2 to 5. We also consider the problem of packing congruent balls in ℝ n , for which we give a closed formula for the packing density as well. Again we observe that our family contains optimal configurations, this time densest packings in dimensions 2 and 3.","lang":"eng"}],"publication":"Rainbow of Computer Science","ddc":["000"],"file":[{"file_id":"4640","file_name":"IST-2016-539-v1+1_2011-B-01-CoveringPacking.pdf","date_updated":"2020-07-14T12:46:16Z","file_size":436875,"relation":"main_file","date_created":"2018-12-12T10:07:42Z","content_type":"application/pdf","creator":"system","access_level":"open_access","checksum":"aaf22b4d7bd4277ffe8db532119cf474"}],"user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","type":"book_chapter","title":"Covering and packing with spheres by diagonal distortion in R^n","year":"2011","publication_status":"published","date_published":"2011-05-03T00:00:00Z","publisher":"Springer","quality_controlled":"1","series_title":"Dedicated to Hermann Maurer on the Occasion of His 70th Birthday","page":"20 - 35","alternative_title":["LNCS"],"language":[{"iso":"eng"}],"status":"public","oa_version":"Submitted Version","publist_id":"2427","oa":1,"has_accepted_license":"1","citation":{"ista":"Edelsbrunner H, Kerber M. 2011.Covering and packing with spheres by diagonal distortion in R^n. In: Rainbow of Computer Science. LNCS, vol. 6570, 20–35.","chicago":"Edelsbrunner, Herbert, and Michael Kerber. “Covering and Packing with Spheres by Diagonal Distortion in R^n.” In Rainbow of Computer Science, edited by Cristian Calude, Grzegorz Rozenberg, and Arto Salomaa, 6570:20–35. Dedicated to Hermann Maurer on the Occasion of His 70th Birthday. Springer, 2011. https://doi.org/10.1007/978-3-642-19391-0_2.","ama":"Edelsbrunner H, Kerber M. Covering and packing with spheres by diagonal distortion in R^n. In: Calude C, Rozenberg G, Salomaa A, eds. Rainbow of Computer Science. Vol 6570. Dedicated to Hermann Maurer on the Occasion of His 70th Birthday. Springer; 2011:20-35. doi:10.1007/978-3-642-19391-0_2","short":"H. Edelsbrunner, M. Kerber, in:, C. Calude, G. Rozenberg, A. Salomaa (Eds.), Rainbow of Computer Science, Springer, 2011, pp. 20–35.","mla":"Edelsbrunner, Herbert, and Michael Kerber. “Covering and Packing with Spheres by Diagonal Distortion in R^n.” Rainbow of Computer Science, edited by Cristian Calude et al., vol. 6570, Springer, 2011, pp. 20–35, doi:10.1007/978-3-642-19391-0_2.","apa":"Edelsbrunner, H., & Kerber, M. (2011). Covering and packing with spheres by diagonal distortion in R^n. In C. Calude, G. Rozenberg, & A. Salomaa (Eds.), Rainbow of Computer Science (Vol. 6570, pp. 20–35). Springer. https://doi.org/10.1007/978-3-642-19391-0_2","ieee":"H. Edelsbrunner and M. Kerber, “Covering and packing with spheres by diagonal distortion in R^n,” in Rainbow of Computer Science, vol. 6570, C. Calude, G. Rozenberg, and A. Salomaa, Eds. Springer, 2011, pp. 20–35."},"pubrep_id":"539","editor":[{"full_name":"Calude, Cristian","last_name":"Calude","first_name":"Cristian"},{"last_name":"Rozenberg","first_name":"Grzegorz","full_name":"Rozenberg, Grzegorz"},{"first_name":"Arto","last_name":"Salomaa","full_name":"Salomaa, Arto"}],"department":[{"_id":"HeEd"}],"month":"05","day":"03","intvolume":" 6570","date_updated":"2021-01-12T07:52:15Z","author":[{"orcid":"0000-0002-9823-6833","full_name":"Edelsbrunner, Herbert","last_name":"Edelsbrunner","id":"3FB178DA-F248-11E8-B48F-1D18A9856A87","first_name":"Herbert"},{"first_name":"Michael","id":"36E4574A-F248-11E8-B48F-1D18A9856A87","last_name":"Kerber","full_name":"Kerber, Michael","orcid":"0000-0002-8030-9299"}],"_id":"3796","date_created":"2018-12-11T12:05:13Z","file_date_updated":"2020-07-14T12:46:16Z","volume":6570},{"oa":1,"publist_id":"7443","oa_version":"Preprint","extern":"1","status":"public","language":[{"iso":"eng"}],"article_processing_charge":"No","quality_controlled":"1","publisher":"American Physical Society","date_published":"2011-07-21T00:00:00Z","publication_status":"published","year":"2011","main_file_link":[{"url":"https://arxiv.org/abs/1003.2233","open_access":"1"}],"title":"STM imaging of a bound state along a step on the surface of the topological insulator Bi2Te3","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","type":"journal_article","publication":"Physical Review B - Condensed Matter and Materials Physics","abstract":[{"text":"We present a detailed study of the local density of states (LDOS) associated with the surface-state band near a step edge of the strong topological insulator Bi2Te3 and reveal a one-dimensional bound state that runs parallel to the step edge and is bound to it at some characteristic distance. This bound state is clearly observed in the bulk gap region, while it becomes entangled with the oscillations of the warped surface band at high energy, and with the valence-band states near the Dirac point. We obtain excellent fits to theoretical predictions [Alpichshev, 2011] that properly incorporate the three-dimensional nature of the problem to the surface state. Fitting the data at different energies, we can recalculate the LDOS originating from the Dirac band without the contribution of the bulk bands or incoherent tunneling effects. ","lang":"eng"}],"doi":"10.1103/PhysRevB.84.041104","volume":84,"issue":"4","date_created":"2018-12-11T11:46:10Z","_id":"386","article_type":"original","author":[{"first_name":"Zhanybek","id":"45E67A2A-F248-11E8-B48F-1D18A9856A87","last_name":"Alpichshev","full_name":"Alpichshev, Zhanybek","orcid":"0000-0002-7183-5203"},{"first_name":"J G","last_name":"Analytis","full_name":"Analytis, J G"},{"full_name":"Chu, J H","last_name":"Chu","first_name":"J H"},{"full_name":"Fisher, I R","last_name":"Fisher","first_name":"I R"},{"last_name":"Kapitulnik","first_name":"A","full_name":"Kapitulnik, A"}],"date_updated":"2021-01-12T07:52:44Z","arxiv":1,"intvolume":" 84","day":"21","month":"07","external_id":{"arxiv":["1003.2233"]},"citation":{"apa":"Alpichshev, Z., Analytis, J. G., Chu, J. H., Fisher, I. R., & Kapitulnik, A. (2011). STM imaging of a bound state along a step on the surface of the topological insulator Bi2Te3. Physical Review B - Condensed Matter and Materials Physics. American Physical Society. https://doi.org/10.1103/PhysRevB.84.041104","mla":"Alpichshev, Zhanybek, et al. “STM Imaging of a Bound State along a Step on the Surface of the Topological Insulator Bi2Te3.” Physical Review B - Condensed Matter and Materials Physics, vol. 84, no. 4, American Physical Society, 2011, doi:10.1103/PhysRevB.84.041104.","short":"Z. Alpichshev, J.G. Analytis, J.H. Chu, I.R. Fisher, A. Kapitulnik, Physical Review B - Condensed Matter and Materials Physics 84 (2011).","chicago":"Alpichshev, Zhanybek, J G Analytis, J H Chu, I R Fisher, and A Kapitulnik. “STM Imaging of a Bound State along a Step on the Surface of the Topological Insulator Bi2Te3.” Physical Review B - Condensed Matter and Materials Physics. American Physical Society, 2011. https://doi.org/10.1103/PhysRevB.84.041104.","ama":"Alpichshev Z, Analytis JG, Chu JH, Fisher IR, Kapitulnik A. STM imaging of a bound state along a step on the surface of the topological insulator Bi2Te3. Physical Review B - Condensed Matter and Materials Physics. 2011;84(4). doi:10.1103/PhysRevB.84.041104","ista":"Alpichshev Z, Analytis JG, Chu JH, Fisher IR, Kapitulnik A. 2011. STM imaging of a bound state along a step on the surface of the topological insulator Bi2Te3. Physical Review B - Condensed Matter and Materials Physics. 84(4).","ieee":"Z. Alpichshev, J. G. Analytis, J. H. Chu, I. R. Fisher, and A. Kapitulnik, “STM imaging of a bound state along a step on the surface of the topological insulator Bi2Te3,” Physical Review B - Condensed Matter and Materials Physics, vol. 84, no. 4. American Physical Society, 2011."}},{"date_created":"2018-12-11T12:06:09Z","language":[{"iso":"eng"}],"_id":"3965","volume":16,"issue":"2.2","publist_id":"2161","oa_version":"None","status":"public","date_published":"2011-05-01T00:00:00Z","publication_status":"published","year":"2011","date_updated":"2021-01-12T07:53:31Z","page":"1 - 13","quality_controlled":"1","author":[{"full_name":"Wang, Bei","last_name":"Wang","first_name":"Bei"},{"full_name":"Edelsbrunner, Herbert","orcid":"0000-0002-9823-6833","id":"3FB178DA-F248-11E8-B48F-1D18A9856A87","last_name":"Edelsbrunner","first_name":"Herbert"},{"full_name":"Morozov, Dmitriy","last_name":"Morozov","first_name":"Dmitriy"}],"publisher":"ACM","month":"05","department":[{"_id":"HeEd"}],"title":"Computing elevation maxima by searching the Gauss sphere","intvolume":" 16","type":"journal_article","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","day":"01","scopus_import":1,"doi":"10.1145/1963190.1970375","citation":{"apa":"Wang, B., Edelsbrunner, H., & Morozov, D. (2011). Computing elevation maxima by searching the Gauss sphere. Journal of Experimental Algorithmics. ACM. https://doi.org/10.1145/1963190.1970375","mla":"Wang, Bei, et al. “Computing Elevation Maxima by Searching the Gauss Sphere.” Journal of Experimental Algorithmics, vol. 16, no. 2.2, ACM, 2011, pp. 1–13, doi:10.1145/1963190.1970375.","short":"B. Wang, H. Edelsbrunner, D. Morozov, Journal of Experimental Algorithmics 16 (2011) 1–13.","ama":"Wang B, Edelsbrunner H, Morozov D. Computing elevation maxima by searching the Gauss sphere. Journal of Experimental Algorithmics. 2011;16(2.2):1-13. doi:10.1145/1963190.1970375","ista":"Wang B, Edelsbrunner H, Morozov D. 2011. Computing elevation maxima by searching the Gauss sphere. Journal of Experimental Algorithmics. 16(2.2), 1–13.","chicago":"Wang, Bei, Herbert Edelsbrunner, and Dmitriy Morozov. “Computing Elevation Maxima by Searching the Gauss Sphere.” Journal of Experimental Algorithmics. ACM, 2011. https://doi.org/10.1145/1963190.1970375.","ieee":"B. Wang, H. Edelsbrunner, and D. Morozov, “Computing elevation maxima by searching the Gauss sphere,” Journal of Experimental Algorithmics, vol. 16, no. 2.2. ACM, pp. 1–13, 2011."},"publication":"Journal of Experimental Algorithmics","abstract":[{"text":"The elevation function on a smoothly embedded 2-manifold in R-3 reflects the multiscale topography of cavities and protrusions as local maxima. The function has been useful in identifying coarse docking configurations for protein pairs. Transporting the concept from the smooth to the piecewise linear category, this paper describes an algorithm for finding all local maxima. While its worst-case running time is the same as of the algorithm used in prior work, its performance in practice is orders of magnitudes superior. We cast light on this improvement by relating the running time to the total absolute Gaussian curvature of the 2-manifold.","lang":"eng"}]},{"intvolume":" 18","day":"15","pmid":1,"month":"06","external_id":{"pmid":[" 20627068"]},"citation":{"ama":"Beeckman T, Friml J. Nitrate Contra Auxin: Nutrient Sensing by roots. Developmental Cell. 2010;18(6):877-878. doi:10.1016/j.devcel.2010.05.020","ista":"Beeckman T, Friml J. 2010. Nitrate Contra Auxin: Nutrient Sensing by roots. Developmental Cell. 18(6), 877–878.","chicago":"Beeckman, Tom, and Jiří Friml. “Nitrate Contra Auxin: Nutrient Sensing by Roots.” Developmental Cell. Cell Press, 2010. https://doi.org/10.1016/j.devcel.2010.05.020.","apa":"Beeckman, T., & Friml, J. (2010). Nitrate Contra Auxin: Nutrient Sensing by roots. Developmental Cell. Cell Press. https://doi.org/10.1016/j.devcel.2010.05.020","mla":"Beeckman, Tom, and Jiří Friml. “Nitrate Contra Auxin: Nutrient Sensing by Roots.” Developmental Cell, vol. 18, no. 6, Cell Press, 2010, pp. 877–78, doi:10.1016/j.devcel.2010.05.020.","short":"T. Beeckman, J. Friml, Developmental Cell 18 (2010) 877–878.","ieee":"T. Beeckman and J. Friml, “Nitrate Contra Auxin: Nutrient Sensing by roots,” Developmental Cell, vol. 18, no. 6. Cell Press, pp. 877–878, 2010."},"volume":18,"issue":"6","date_created":"2018-12-11T11:57:41Z","_id":"2442","author":[{"full_name":"Beeckman, Tom","first_name":"Tom","last_name":"Beeckman"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","first_name":"Jirí","full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596"}],"date_updated":"2021-01-12T06:57:31Z","main_file_link":[{"open_access":"1","url":"https://www.ncbi.nlm.nih.gov/pubmed/20627068"}],"title":"Nitrate Contra Auxin: Nutrient Sensing by roots","type":"journal_article","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","publication":"Developmental Cell","abstract":[{"text":"In a new study published in this issue of Developmental Cell, Krouk et al. reveal a surprising mechanism by which plant root systems adapt their architecture for soil exploitation. The dual transporter NRT1.1 uses both nitrate and the plant hormone auxin as substrates, enabling soil nitrate availability to regulate auxin-driven lateral root development.","lang":"eng"}],"doi":"10.1016/j.devcel.2010.05.020","publist_id":"4461","oa":1,"oa_version":"Published Version","status":"public","extern":"1","language":[{"iso":"eng"}],"page":"877 - 878","quality_controlled":"1","publisher":"Cell Press","date_published":"2010-06-15T00:00:00Z","publication_status":"published","year":"2010"},{"status":"public","extern":1,"issue":"8","publist_id":"4398","volume":107,"_id":"2503","date_created":"2018-12-11T11:58:03Z","author":[{"first_name":"Yuko","last_name":"Fukata","full_name":"Fukata, Yuko"},{"first_name":"Kathryn","last_name":"Lovero","full_name":"Lovero, Kathryn L"},{"last_name":"Iwanaga","first_name":"Tsuyoshi","full_name":"Iwanaga, Tsuyoshi"},{"full_name":"Watanabe, Atsushi","last_name":"Watanabe","first_name":"Atsushi"},{"full_name":"Yokoi, Norihiko","last_name":"Yokoi","first_name":"Norihiko"},{"last_name":"Tabuchi","first_name":"Katsuhiko","full_name":"Tabuchi, Katsuhiko"},{"last_name":"Shigemoto","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","first_name":"Ryuichi","orcid":"0000-0001-8761-9444","full_name":"Ryuichi Shigemoto"},{"full_name":"Nicoll, Roger A","first_name":"Roger","last_name":"Nicoll"},{"last_name":"Fukata","first_name":"Masaki","full_name":"Fukata, Masaki"}],"publisher":"National Academy of Sciences","quality_controlled":0,"page":"3799 - 3804","date_updated":"2021-01-12T06:57:53Z","publication_status":"published","year":"2010","date_published":"2010-02-23T00:00:00Z","day":"23","type":"journal_article","title":"Disruption of LGI1-linked synaptic complex causes abnormal synaptic transmission and epilepsy","intvolume":" 107","month":"02","abstract":[{"text":"Epilepsy is a devastating and poorly understood disease. Mutations in a secreted neuronal protein, leucine-rich glioma inactivated 1 (LGI1), were reported in patients with an inherited form of human epilepsy, autosomal dominant partial epilepsy with auditory features (ADPEAF). Here, we report an essential role of LGI1 as an antiepileptogenic ligand. We find that loss of LGI1 in mice (LGI1-/-) causes lethal epilepsy, which is specifically rescued by the neuronal expression of LGI1 transgene, but not LGI3. Moreover, heterozygous mice for the LGI1 mutation (LGI1+/-) show lowered seizure thresholds. Extracellularly secreted LGI1 links two epilepsy-related receptors, ADAM22 and ADAM23, in the brain and organizes a transsynaptic protein complex that includes presynaptic potassium channels and postsynaptic AMPA receptor scaffolds. A lack of LGI1 disrupts this synaptic protein connection and selectively reduces AMPA receptor-mediated synaptic transmission in the hippocampus. Thus, LGI1 may serve as a major determinant of brain excitation, and the LGI1 gene-targeted mouse provides a good model for human epilepsy.","lang":"eng"}],"publication":"PNAS","citation":{"ama":"Fukata Y, Lovero K, Iwanaga T, et al. Disruption of LGI1-linked synaptic complex causes abnormal synaptic transmission and epilepsy. PNAS. 2010;107(8):3799-3804. doi:10.1073/pnas.0914537107","ista":"Fukata Y, Lovero K, Iwanaga T, Watanabe A, Yokoi N, Tabuchi K, Shigemoto R, Nicoll R, Fukata M. 2010. Disruption of LGI1-linked synaptic complex causes abnormal synaptic transmission and epilepsy. PNAS. 107(8), 3799–3804.","chicago":"Fukata, Yuko, Kathryn Lovero, Tsuyoshi Iwanaga, Atsushi Watanabe, Norihiko Yokoi, Katsuhiko Tabuchi, Ryuichi Shigemoto, Roger Nicoll, and Masaki Fukata. “Disruption of LGI1-Linked Synaptic Complex Causes Abnormal Synaptic Transmission and Epilepsy.” PNAS. National Academy of Sciences, 2010. https://doi.org/10.1073/pnas.0914537107.","short":"Y. Fukata, K. Lovero, T. Iwanaga, A. Watanabe, N. Yokoi, K. Tabuchi, R. Shigemoto, R. Nicoll, M. Fukata, PNAS 107 (2010) 3799–3804.","mla":"Fukata, Yuko, et al. “Disruption of LGI1-Linked Synaptic Complex Causes Abnormal Synaptic Transmission and Epilepsy.” PNAS, vol. 107, no. 8, National Academy of Sciences, 2010, pp. 3799–804, doi:10.1073/pnas.0914537107.","apa":"Fukata, Y., Lovero, K., Iwanaga, T., Watanabe, A., Yokoi, N., Tabuchi, K., … Fukata, M. (2010). Disruption of LGI1-linked synaptic complex causes abnormal synaptic transmission and epilepsy. PNAS. National Academy of Sciences. https://doi.org/10.1073/pnas.0914537107","ieee":"Y. Fukata et al., “Disruption of LGI1-linked synaptic complex causes abnormal synaptic transmission and epilepsy,” PNAS, vol. 107, no. 8. National Academy of Sciences, pp. 3799–3804, 2010."},"doi":"10.1073/pnas.0914537107"},{"publication":"Ultramicroscopy","abstract":[{"lang":"eng","text":"We present a method for immunolabeling of multiple species of membrane proteins with high spatial resolution. It allows differentiation of equally sized very small markers with different chemical compositions, which leads to high labeling efficiency and reduces steric hindrance of closely spaced immunolabeled biomolecules. Markers such as CdSe/ZnS semiconductor quantum dots and colloidal gold particles are distinguished by differential contrast in high-angle annular detector dark-field STEM mode or by EDX microanalysis of their elemental contents. This method was tested by observation of labeled AMPA- and NMDA-type glutamate receptors on sodium-dodecyl-sulfate-digested replica prepared from rat hippocampus. To improve particle visibility and detectability, the replica films were made exclusively with carbon to avoid the high background of conventional platinum/carbon replica. Extension of the method is suggested by detection of 1.4 nm nanogold particles and its potential application in the biological imaging research."}],"doi":"10.1016/j.ultramic.2010.01.016","citation":{"ieee":"A. Loukanov, N. Kamasawa, R. Danev, R. Shigemoto, and K. Nagayama, “Immunolocalization of multiple membrane proteins on a carbon replica with STEM and EDX,” Ultramicroscopy, vol. 110, no. 4. Elsevier, pp. 366–374, 2010.","short":"A. Loukanov, N. Kamasawa, R. Danev, R. Shigemoto, K. Nagayama, Ultramicroscopy 110 (2010) 366–374.","mla":"Loukanov, Alexandre, et al. “Immunolocalization of Multiple Membrane Proteins on a Carbon Replica with STEM and EDX.” Ultramicroscopy, vol. 110, no. 4, Elsevier, 2010, pp. 366–74, doi:10.1016/j.ultramic.2010.01.016.","apa":"Loukanov, A., Kamasawa, N., Danev, R., Shigemoto, R., & Nagayama, K. (2010). Immunolocalization of multiple membrane proteins on a carbon replica with STEM and EDX. Ultramicroscopy. Elsevier. https://doi.org/10.1016/j.ultramic.2010.01.016","ista":"Loukanov A, Kamasawa N, Danev R, Shigemoto R, Nagayama K. 2010. Immunolocalization of multiple membrane proteins on a carbon replica with STEM and EDX. Ultramicroscopy. 110(4), 366–374.","ama":"Loukanov A, Kamasawa N, Danev R, Shigemoto R, Nagayama K. Immunolocalization of multiple membrane proteins on a carbon replica with STEM and EDX. Ultramicroscopy. 2010;110(4):366-374. doi:10.1016/j.ultramic.2010.01.016","chicago":"Loukanov, Alexandre, Naomi Kamasawa, Radostin Danev, Ryuichi Shigemoto, and Kuniaki Nagayama. “Immunolocalization of Multiple Membrane Proteins on a Carbon Replica with STEM and EDX.” Ultramicroscopy. Elsevier, 2010. https://doi.org/10.1016/j.ultramic.2010.01.016."},"title":"Immunolocalization of multiple membrane proteins on a carbon replica with STEM and EDX","intvolume":" 110","day":"01","type":"journal_article","month":"03","page":"366 - 374","quality_controlled":0,"author":[{"first_name":"Alexandre","last_name":"Loukanov","full_name":"Loukanov, Alexandre R"},{"first_name":"Naomi","last_name":"Kamasawa","full_name":"Kamasawa, Naomi"},{"full_name":"Danev, Radostin S","last_name":"Danev","first_name":"Radostin"},{"last_name":"Shigemoto","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","first_name":"Ryuichi","orcid":"0000-0001-8761-9444","full_name":"Ryuichi Shigemoto"},{"last_name":"Nagayama","first_name":"Kuniaki","full_name":"Nagayama, Kuniaki"}],"publisher":"Elsevier","date_published":"2010-03-01T00:00:00Z","year":"2010","publication_status":"published","date_updated":"2021-01-12T06:57:53Z","volume":110,"publist_id":"4397","issue":"4","status":"public","extern":1,"date_created":"2018-12-11T11:58:03Z","_id":"2504"},{"abstract":[{"text":"Cbln1, secreted from cerebellar granule cells, and the orphan glutamate receptor 52 (GluD2), expressed by Purkinje cells, are essential for synapse integrity between these neurons in adult mice. Nevertheless, no endogenous binding partners for these molecules have been identified. We found that Cblnl binds directly to the N-terminal domain of GluD2. GluD2 expression by postsynaptic cells, combined with exogenously applied Cbln1, was necessary and sufficient to induce new synapses in vitro and in the adult cerebellum in vivo. Further, beads coated with recombinant Cbln1 directly induced presynaptic differentiation and indirectly caused clustering of postsynaptic molecules via GluD2. These results indicate that the Cbln1-GluD2 complex is a unique synapse organizer that acts bidirectionally on both pre- and postsynaptic components.","lang":"eng"}],"publication":"Science","citation":{"ieee":"K. Matsuda et al., “Cbln1 is a ligand for an orphan glutamate receptor δ2, a bidirectional synapse organizer,” Science, vol. 328, no. 5976. American Association for the Advancement of Science, pp. 363–368, 2010.","ama":"Matsuda K, Miura E, Miyazaki T, et al. Cbln1 is a ligand for an orphan glutamate receptor δ2, a bidirectional synapse organizer. Science. 2010;328(5976):363-368. doi:10.1126/science.1185152","chicago":"Matsuda, Keiko, Eriko Miura, Taisuke Miyazaki, Wataru Kakegawa, Kyoichi Emi, Sakae Narumi, Yugo Fukazawa, et al. “Cbln1 Is a Ligand for an Orphan Glutamate Receptor Δ2, a Bidirectional Synapse Organizer.” Science. American Association for the Advancement of Science, 2010. https://doi.org/10.1126/science.1185152.","ista":"Matsuda K, Miura E, Miyazaki T, Kakegawa W, Emi K, Narumi S, Fukazawa Y, Ito Lshida A, Kondo T, Shigemoto R, Watanabe M, Yuzaki M. 2010. Cbln1 is a ligand for an orphan glutamate receptor δ2, a bidirectional synapse organizer. Science. 328(5976), 363–368.","apa":"Matsuda, K., Miura, E., Miyazaki, T., Kakegawa, W., Emi, K., Narumi, S., … Yuzaki, M. (2010). Cbln1 is a ligand for an orphan glutamate receptor δ2, a bidirectional synapse organizer. Science. American Association for the Advancement of Science. https://doi.org/10.1126/science.1185152","mla":"Matsuda, Keiko, et al. “Cbln1 Is a Ligand for an Orphan Glutamate Receptor Δ2, a Bidirectional Synapse Organizer.” Science, vol. 328, no. 5976, American Association for the Advancement of Science, 2010, pp. 363–68, doi:10.1126/science.1185152.","short":"K. Matsuda, E. Miura, T. Miyazaki, W. Kakegawa, K. Emi, S. Narumi, Y. Fukazawa, A. Ito Lshida, T. Kondo, R. Shigemoto, M. Watanabe, M. Yuzaki, Science 328 (2010) 363–368."},"doi":"10.1126/science.1185152","intvolume":" 328","title":"Cbln1 is a ligand for an orphan glutamate receptor δ2, a bidirectional synapse organizer","type":"journal_article","day":"16","month":"04","quality_controlled":0,"publisher":"American Association for the Advancement of Science","author":[{"last_name":"Matsuda","first_name":"Keiko","full_name":"Matsuda, Keiko"},{"last_name":"Miura","first_name":"Eriko","full_name":"Miura, Eriko"},{"full_name":"Miyazaki, Taisuke","last_name":"Miyazaki","first_name":"Taisuke"},{"first_name":"Wataru","last_name":"Kakegawa","full_name":"Kakegawa, Wataru"},{"first_name":"Kyoichi","last_name":"Emi","full_name":"Emi, Kyoichi"},{"full_name":"Narumi, Sakae","last_name":"Narumi","first_name":"Sakae"},{"first_name":"Yugo","last_name":"Fukazawa","full_name":"Fukazawa, Yugo"},{"full_name":"Ito-lshida, Aya","first_name":"Aya","last_name":"Ito Lshida"},{"full_name":"Kondo, Tetsuro","last_name":"Kondo","first_name":"Tetsuro"},{"id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","last_name":"Shigemoto","first_name":"Ryuichi","full_name":"Ryuichi Shigemoto","orcid":"0000-0001-8761-9444"},{"first_name":"Masahiko","last_name":"Watanabe","full_name":"Watanabe, Masahiko"},{"last_name":"Yuzaki","first_name":"Michisuke","full_name":"Yuzaki, Michisuke"}],"page":"363 - 368","year":"2010","publication_status":"published","date_updated":"2021-01-12T06:57:53Z","date_published":"2010-04-16T00:00:00Z","extern":1,"status":"public","volume":328,"issue":"5976","publist_id":"4396","date_created":"2018-12-11T11:58:04Z","_id":"2505"},{"date_published":"2010-11-01T00:00:00Z","publication_status":"published","year":"2010","date_updated":"2021-01-12T06:57:54Z","page":"243 - 259","quality_controlled":0,"author":[{"last_name":"Furuya","first_name":"Sonoko","full_name":"Furuya, Sonoko"},{"first_name":"Kishio","last_name":"Furuya","full_name":"Furuya, Kishio"},{"full_name":"Ryuichi Shigemoto","orcid":"0000-0001-8761-9444","first_name":"Ryuichi","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","last_name":"Shigemoto"},{"full_name":"Sokabe, Masahiro","last_name":"Sokabe","first_name":"Masahiro"}],"publisher":"Springer","date_created":"2018-12-11T11:58:04Z","_id":"2506","volume":342,"issue":"2","publist_id":"4395","status":"public","extern":1,"doi":"10.1007/s00441-010-1056-7","citation":{"ama":"Furuya S, Furuya K, Shigemoto R, Sokabe M. Localization of NK1 receptors and roles of substance-P in subepithelial fibroblasts of rat intestinal villi. Cell and Tissue Research. 2010;342(2):243-259. doi:10.1007/s00441-010-1056-7","ista":"Furuya S, Furuya K, Shigemoto R, Sokabe M. 2010. Localization of NK1 receptors and roles of substance-P in subepithelial fibroblasts of rat intestinal villi. Cell and Tissue Research. 342(2), 243–259.","chicago":"Furuya, Sonoko, Kishio Furuya, Ryuichi Shigemoto, and Masahiro Sokabe. “Localization of NK1 Receptors and Roles of Substance-P in Subepithelial Fibroblasts of Rat Intestinal Villi.” Cell and Tissue Research. Springer, 2010. https://doi.org/10.1007/s00441-010-1056-7.","mla":"Furuya, Sonoko, et al. “Localization of NK1 Receptors and Roles of Substance-P in Subepithelial Fibroblasts of Rat Intestinal Villi.” Cell and Tissue Research, vol. 342, no. 2, Springer, 2010, pp. 243–59, doi:10.1007/s00441-010-1056-7.","short":"S. Furuya, K. Furuya, R. Shigemoto, M. Sokabe, Cell and Tissue Research 342 (2010) 243–259.","apa":"Furuya, S., Furuya, K., Shigemoto, R., & Sokabe, M. (2010). Localization of NK1 receptors and roles of substance-P in subepithelial fibroblasts of rat intestinal villi. Cell and Tissue Research. Springer. https://doi.org/10.1007/s00441-010-1056-7","ieee":"S. Furuya, K. Furuya, R. Shigemoto, and M. Sokabe, “Localization of NK1 receptors and roles of substance-P in subepithelial fibroblasts of rat intestinal villi,” Cell and Tissue Research, vol. 342, no. 2. Springer, pp. 243–259, 2010."},"publication":"Cell and Tissue Research","abstract":[{"lang":"eng","text":"Subepithelial fibroblasts of the intestinal villi, which form a contractile cellular network beneath the epithelium, are in close contact with epithelial cells, nerve varicosities, capillaries, smooth muscles and immune cells, and secrete extracellular matrix molecules, growth factors and cytokines, etc. Cultured subepithelial fibroblasts of the rat duodenal villi display various receptors such as endothelins, ATP, substance-P and bradykinin, and release ATP in response to mechanical stimulation. In this study, the presence of functional NK1 receptors (NK1R) was pharmacologically confirmed in primary culture by Ca 2+ measurement, and the effects of substance-P were measured in an acute preparation of epithelium-free duodenal villi from 2- to 3-week-old rats using a two-photon laser microscope. Substance-P elicited an increase in the intracellular Ca 2+ concentration and contraction of the subepithelial fibroblasts in culture and the isolated villi. The localization of NK1R and substance-P in the villi was examined by light and electron microscopic immunohistochemistry. NK1R-like immunoreactivity was intensely localized on the plasma membrane of villous subepithelial fibroblasts in 10-day- to 4-week-old rats and mice and was decreased or absent in adulthood. The pericryptal fibroblasts of the small and large intestine were NK1R immuno-negative. These villous subepithelial fibroblasts form synapse-like structures with both substance-P-immunopositive and -immunonegative nerve varicosities. Here, we propose that the mutual interaction between villous subepithelial fibroblasts and afferent neurons via substance-P and ATP plays important roles in the maturation of the structure and function of the small intestine."}],"month":"11","intvolume":" 342","title":"Localization of NK1 receptors and roles of substance-P in subepithelial fibroblasts of rat intestinal villi","type":"journal_article","day":"01"},{"page":"4362 - 4374","quality_controlled":0,"author":[{"first_name":"Laxmi","last_name":"Parajuli","full_name":"Parajuli, Laxmi K"},{"first_name":"Yugo","last_name":"Fukazawa","full_name":"Fukazawa, Yugo"},{"first_name":"Masahiko","last_name":"Watanabe","full_name":"Watanabe, Masahiko"},{"first_name":"Ryuichi","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","last_name":"Shigemoto","full_name":"Ryuichi Shigemoto","orcid":"0000-0001-8761-9444"}],"publisher":"Wiley-Blackwell","date_published":"2010-11-01T00:00:00Z","year":"2010","publication_status":"published","date_updated":"2021-01-12T06:57:54Z","volume":518,"issue":"21","publist_id":"4394","status":"public","extern":1,"date_created":"2018-12-11T11:58:04Z","_id":"2507","publication":"Journal of Comparative Neurology","abstract":[{"text":"T-type calcium channels play a pivotal role in regulating neural membrane excitability in the nervous system. However, the precise subcellular distributions of T-type channel subunits and their implication for membrane excitability are not well understood. Here we investigated the subcellular distribution of the α1G subunit of the calcium channel which is expressed highly in the mouse dorsal lateral geniculate nucleus (dLGN). Light microscopic analysis demonstrated that dLGN exhibits intense immunoperoxidase reactivity for the α1G subunit. Electron microscopic observation showed that the labeling was present in both the relay cells and interneurons and was found in the somatodendritic, but not axonal, domains of these cells. Most of the immunogold particles for the α1G subunit were either associated with the plasma membrane or the intracellular membranes. Reconstruction analysis of serial electron microscopic images revealed that the intensity of the intracellular labeling exhibited a gradient such that the labeling density was higher in the proximal dendrite and progressively decreased towards the distal dendrite. In contrast, the plasma membrane-associated particles were distributed with a uniform density over the somatodendritic surface of dLGN cells. The labeling density in the relay cell plasma membrane was about 3-fold higher than that of the interneurons. These results provide ultrastructural evidence for cell-type-specific expression levels and for uniform expression density of the α1G subunit over the plasma membrane of dLGN cells.","lang":"eng"}],"doi":"10.1002/cne.22461","citation":{"ieee":"L. Parajuli, Y. Fukazawa, M. Watanabe, and R. Shigemoto, “Subcellular distribution of α1G subunit of T-type calcium channel in the mouse dorsal lateral geniculate nucleus,” Journal of Comparative Neurology, vol. 518, no. 21. Wiley-Blackwell, pp. 4362–4374, 2010.","chicago":"Parajuli, Laxmi, Yugo Fukazawa, Masahiko Watanabe, and Ryuichi Shigemoto. “Subcellular Distribution of Α1G Subunit of T-Type Calcium Channel in the Mouse Dorsal Lateral Geniculate Nucleus.” Journal of Comparative Neurology. Wiley-Blackwell, 2010. https://doi.org/10.1002/cne.22461.","ista":"Parajuli L, Fukazawa Y, Watanabe M, Shigemoto R. 2010. Subcellular distribution of α1G subunit of T-type calcium channel in the mouse dorsal lateral geniculate nucleus. Journal of Comparative Neurology. 518(21), 4362–4374.","ama":"Parajuli L, Fukazawa Y, Watanabe M, Shigemoto R. Subcellular distribution of α1G subunit of T-type calcium channel in the mouse dorsal lateral geniculate nucleus. Journal of Comparative Neurology. 2010;518(21):4362-4374. doi:10.1002/cne.22461","apa":"Parajuli, L., Fukazawa, Y., Watanabe, M., & Shigemoto, R. (2010). Subcellular distribution of α1G subunit of T-type calcium channel in the mouse dorsal lateral geniculate nucleus. Journal of Comparative Neurology. Wiley-Blackwell. https://doi.org/10.1002/cne.22461","short":"L. Parajuli, Y. Fukazawa, M. Watanabe, R. Shigemoto, Journal of Comparative Neurology 518 (2010) 4362–4374.","mla":"Parajuli, Laxmi, et al. “Subcellular Distribution of Α1G Subunit of T-Type Calcium Channel in the Mouse Dorsal Lateral Geniculate Nucleus.” Journal of Comparative Neurology, vol. 518, no. 21, Wiley-Blackwell, 2010, pp. 4362–74, doi:10.1002/cne.22461."},"intvolume":" 518","title":"Subcellular distribution of α1G subunit of T-type calcium channel in the mouse dorsal lateral geniculate nucleus","day":"01","type":"journal_article","month":"11"},{"status":"public","extern":1,"volume":30,"publist_id":"4393","issue":"47","date_created":"2018-12-11T11:58:05Z","_id":"2508","quality_controlled":0,"author":[{"last_name":"Atherton","first_name":"Jeremy","full_name":"Atherton, Jeremy F"},{"first_name":"Katsunori","last_name":"Kitano","full_name":"Kitano, Katsunori"},{"full_name":"Baufreton, Jérôme","first_name":"Jérôme","last_name":"Baufreton"},{"full_name":"Fan, Kai","last_name":"Fan","first_name":"Kai"},{"full_name":"Wokosin, David L","first_name":"David","last_name":"Wokosin"},{"full_name":"Tkatch, Tatiana","first_name":"Tatiana","last_name":"Tkatch"},{"full_name":"Ryuichi Shigemoto","orcid":"0000-0001-8761-9444","first_name":"Ryuichi","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","last_name":"Shigemoto"},{"last_name":"Surmeier","first_name":"James","full_name":"Surmeier, James D"},{"full_name":"Bevan, Mark D","last_name":"Bevan","first_name":"Mark"}],"publisher":"Society for Neuroscience","page":"16025 - 16040","year":"2010","publication_status":"published","date_updated":"2021-01-12T06:57:54Z","date_published":"2010-11-24T00:00:00Z","title":"Selective participation of somatodendritic HCN channels in inhibitory but not excitatory synaptic integration in neurons of the subthalamic nucleus","intvolume":" 30","type":"journal_article","day":"24","month":"11","abstract":[{"text":"The activity patterns of subthalamic nucleus (STN) neurons are intimately linked to motor function and dysfunction and arise through the complex interaction of intrinsic properties and inhibitory and excitatory synaptic inputs. In many neurons, hyperpolarization-activated cyclic nucleotide-gated (HCN) channels play key roles in intrinsic excitability and synaptic integration both under normal conditions and in disease states. However, in STN neurons, which strongly express HCN channels, their roles remain relatively obscure. To address this deficit, complementary molecular and cellular electrophysiological, imaging, and computational approaches were applied to the rat STN. Molecular profiling demonstrated that individual STN neurons express mRNA encoding several HCN subunits, with HCN2 and 3 being the most abundant. Light and electron microscopic analysis showed that HCN2 subunits are strongly expressed and distributed throughout the somatodendritic plasma membrane. Voltage-, current-, and dynamic-clamp analysis, two-photon Ca 2+ imaging, and computational modeling revealed that HCN channels are activated by GABA A receptor-mediated inputs and thus limit synaptic hyperpolarization and deinactivation of low-voltage-activated Ca 2+ channels. Although HCN channels also limited the temporal summation of EPSPs, generated through two-photon uncaging of glutamate, this action was largely shunted by GABAergic inhibition that was necessary for HCN channel activation. Together the data demonstrate that HCN channels in STN neurons selectively counteract GABA A receptor-mediated inhibition arising from the globus pallidus and thus promote single-spike activity rather than rebound burst firing. ","lang":"eng"}],"publication":"Journal of Neuroscience","citation":{"ieee":"J. Atherton et al., “Selective participation of somatodendritic HCN channels in inhibitory but not excitatory synaptic integration in neurons of the subthalamic nucleus,” Journal of Neuroscience, vol. 30, no. 47. Society for Neuroscience, pp. 16025–16040, 2010.","chicago":"Atherton, Jeremy, Katsunori Kitano, Jérôme Baufreton, Kai Fan, David Wokosin, Tatiana Tkatch, Ryuichi Shigemoto, James Surmeier, and Mark Bevan. “Selective Participation of Somatodendritic HCN Channels in Inhibitory but Not Excitatory Synaptic Integration in Neurons of the Subthalamic Nucleus.” Journal of Neuroscience. Society for Neuroscience, 2010. https://doi.org/10.1523/JNEUROSCI.3898-10.2010.","ista":"Atherton J, Kitano K, Baufreton J, Fan K, Wokosin D, Tkatch T, Shigemoto R, Surmeier J, Bevan M. 2010. Selective participation of somatodendritic HCN channels in inhibitory but not excitatory synaptic integration in neurons of the subthalamic nucleus. Journal of Neuroscience. 30(47), 16025–16040.","ama":"Atherton J, Kitano K, Baufreton J, et al. Selective participation of somatodendritic HCN channels in inhibitory but not excitatory synaptic integration in neurons of the subthalamic nucleus. Journal of Neuroscience. 2010;30(47):16025-16040. doi:10.1523/JNEUROSCI.3898-10.2010","mla":"Atherton, Jeremy, et al. “Selective Participation of Somatodendritic HCN Channels in Inhibitory but Not Excitatory Synaptic Integration in Neurons of the Subthalamic Nucleus.” Journal of Neuroscience, vol. 30, no. 47, Society for Neuroscience, 2010, pp. 16025–40, doi:10.1523/JNEUROSCI.3898-10.2010.","apa":"Atherton, J., Kitano, K., Baufreton, J., Fan, K., Wokosin, D., Tkatch, T., … Bevan, M. (2010). Selective participation of somatodendritic HCN channels in inhibitory but not excitatory synaptic integration in neurons of the subthalamic nucleus. Journal of Neuroscience. Society for Neuroscience. https://doi.org/10.1523/JNEUROSCI.3898-10.2010","short":"J. Atherton, K. Kitano, J. Baufreton, K. Fan, D. Wokosin, T. Tkatch, R. Shigemoto, J. Surmeier, M. Bevan, Journal of Neuroscience 30 (2010) 16025–16040."},"doi":"10.1523/JNEUROSCI.3898-10.2010"},{"publication":"European Journal of Neuroscience","abstract":[{"text":"Hippocampal CA1 pyramidal cells, which receive γ-aminobutyric acid (GABA)ergic input from at least 18 types of presynaptic neuron, express 14 subunits of the pentameric GABAA receptor. The relative contribution of any subunit to synaptic and extrasynaptic receptors influences the dynamics of GABA and drug actions. Synaptic receptors mediate phasic GABA-evoked conductance and extrasynaptic receptors contribute to a tonic conductance. We used freeze-fracture replica-immunogold labelling, a sensitive quantitative immunocytochemical method, to detect synaptic and extrasynaptic pools of the alpha1, alpha2 and beta3 subunits. Antibodies to the cytoplasmic loop of the subunits showed immunogold particles concentrated on distinct clusters of intramembrane particles (IMPs) on the cytoplasmic face of the plasma membrane on the somata, dendrites and axon initial segments, with an abrupt decrease in labelling at the edge of the IMP cluster. Neuroligin-2, a GABAergic synapse-specific adhesion molecule, co-labels all beta3 subunit-rich IMP clusters, therefore we considered them synapses. Double-labelling for two subunits showed that virtually all somatic synapses contain the alpha1, alpha2 and beta3 subunits. The extrasynaptic plasma membrane of the somata, dendrites and dendritic spines showed low-density immunolabelling. Synaptic labelling densities on somata for the alpha1, alpha2 and beta3 subunits were 78-132, 94 and 79 times higher than on the extrasynaptic membranes, respectively. As GABAergic synapses occupy 0.72% of the soma surface, the fraction of synaptic labelling was 33-48 (alpha1), 40 (alpha2) and 36 (beta3)% of the total somatic surface immunolabelling. Assuming similar antibody access to all receptors, about 60% of these subunits are in extrasynaptic receptors.","lang":"eng"}],"doi":"10.1111/j.1460-9568.2010.07473.x","citation":{"short":"Y. Kasugai, J. Swinny, J. Roberts, Y. Dalezios, Y. Fukazawa, W. Sieghart, R. Shigemoto, P. Somogyi, European Journal of Neuroscience 32 (2010) 1868–1888.","apa":"Kasugai, Y., Swinny, J., Roberts, J., Dalezios, Y., Fukazawa, Y., Sieghart, W., … Somogyi, P. (2010). Quantitative localisation of synaptic and extrasynaptic GABAA receptor subunits on hippocampal pyramidal cells by freeze-fracture replica immunolabelling. European Journal of Neuroscience. Wiley-Blackwell. https://doi.org/10.1111/j.1460-9568.2010.07473.x","mla":"Kasugai, Yu, et al. “Quantitative Localisation of Synaptic and Extrasynaptic GABAA Receptor Subunits on Hippocampal Pyramidal Cells by Freeze-Fracture Replica Immunolabelling.” European Journal of Neuroscience, vol. 32, no. 11, Wiley-Blackwell, 2010, pp. 1868–88, doi:10.1111/j.1460-9568.2010.07473.x.","chicago":"Kasugai, Yu, Jerome Swinny, John Roberts, Yannis Dalezios, Yugo Fukazawa, Werner Sieghart, Ryuichi Shigemoto, and Péter Somogyi. “Quantitative Localisation of Synaptic and Extrasynaptic GABAA Receptor Subunits on Hippocampal Pyramidal Cells by Freeze-Fracture Replica Immunolabelling.” European Journal of Neuroscience. Wiley-Blackwell, 2010. https://doi.org/10.1111/j.1460-9568.2010.07473.x.","ista":"Kasugai Y, Swinny J, Roberts J, Dalezios Y, Fukazawa Y, Sieghart W, Shigemoto R, Somogyi P. 2010. Quantitative localisation of synaptic and extrasynaptic GABAA receptor subunits on hippocampal pyramidal cells by freeze-fracture replica immunolabelling. European Journal of Neuroscience. 32(11), 1868–1888.","ama":"Kasugai Y, Swinny J, Roberts J, et al. Quantitative localisation of synaptic and extrasynaptic GABAA receptor subunits on hippocampal pyramidal cells by freeze-fracture replica immunolabelling. European Journal of Neuroscience. 2010;32(11):1868-1888. doi:10.1111/j.1460-9568.2010.07473.x","ieee":"Y. Kasugai et al., “Quantitative localisation of synaptic and extrasynaptic GABAA receptor subunits on hippocampal pyramidal cells by freeze-fracture replica immunolabelling,” European Journal of Neuroscience, vol. 32, no. 11. Wiley-Blackwell, pp. 1868–1888, 2010."},"title":"Quantitative localisation of synaptic and extrasynaptic GABAA receptor subunits on hippocampal pyramidal cells by freeze-fracture replica immunolabelling","intvolume":" 32","type":"journal_article","day":"14","month":"11","page":"1868 - 1888","quality_controlled":0,"author":[{"last_name":"Kasugai","first_name":"Yu","full_name":"Kasugai, Yu"},{"last_name":"Swinny","first_name":"Jerome","full_name":"Swinny, Jerome D"},{"first_name":"John","last_name":"Roberts","full_name":"Roberts, John D"},{"last_name":"Dalezios","first_name":"Yannis","full_name":"Dalezios, Yannis"},{"full_name":"Fukazawa, Yugo","last_name":"Fukazawa","first_name":"Yugo"},{"first_name":"Werner","last_name":"Sieghart","full_name":"Sieghart, Werner C"},{"orcid":"0000-0001-8761-9444","full_name":"Ryuichi Shigemoto","first_name":"Ryuichi","last_name":"Shigemoto","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Somogyi, Péter","first_name":"Péter","last_name":"Somogyi"}],"publisher":"Wiley-Blackwell","date_published":"2010-11-14T00:00:00Z","year":"2010","publication_status":"published","date_updated":"2021-01-12T06:57:55Z","volume":32,"issue":"11","publist_id":"4392","extern":1,"status":"public","date_created":"2018-12-11T11:58:05Z","_id":"2509"},{"intvolume":" 518","title":"Differential postsynaptic compartments in the laterocapsular division of the central nucleus of amygdala for afferents from the parabrachial nucleus and the basolateral nucleus in the rat","type":"journal_article","day":"01","month":"12","abstract":[{"lang":"eng","text":"Neurons in the laterocapsular division of the central nucleus of the amygdala (CeC), which is known as the "nociceptive amygdala," receive glutamatergic inputs from the parabrachial nucleus (PB) and the basolateral nucleus of amygdala (BLA), which convey nociceptive information from the dorsal horn of the spinal cord and polymodal information from the thalamus and cortex, respectively. Here, we examined the ultrastructural properties of PB- and BLA-CeC synapses identified with EGFP-expressing lentivirus in rats. In addition, the density of synaptic AMPA receptors (AMPARs) on CeC neurons was studied by using highly sensitive SDS-digested freeze-fracture replica labeling (SDS-FRL). Afferents from the PB made asymmetrical synapses mainly on dendritic shafts (88%), whereas those from the BLA were on dendritic spines (81%). PB-CeC synapses in dendritic shafts were significantly larger (median 0.072 μm 2) than BLA-CeC synapses in spines (median 0.058 μm 2; P = 0.02). The dendritic shafts that made synapses with PB fibers were also significantly larger than those that made synapses with BLA fibers, indicating that the PB fibers make synapses on more proximal parts of dendrites than the BLA fibers. SDS-FRL revealed that almost all excitatory postsynaptic sites have AMPARs in the CeC. The density of AMPAR-specific gold particles in individual synapses was significantly higher in spine synapses (median 510 particles/μm 2) than in shaft synapses (median 427 particles/μm 2; P = 0.01). These results suggest that distinct synaptic impacts from PB- and BLA-CeC pathways contribute to the integration of nociceptive and polymodal information in the CeC."}],"publication":"Journal of Comparative Neurology","citation":{"ieee":"Y. Dong, Y. Fukazawa, W. Wang, N. Kamasawa, and R. Shigemoto, “Differential postsynaptic compartments in the laterocapsular division of the central nucleus of amygdala for afferents from the parabrachial nucleus and the basolateral nucleus in the rat,” Journal of Comparative Neurology, vol. 518, no. 23. Wiley-Blackwell, pp. 4771–4791, 2010.","short":"Y. Dong, Y. Fukazawa, W. Wang, N. Kamasawa, R. Shigemoto, Journal of Comparative Neurology 518 (2010) 4771–4791.","mla":"Dong, Yu, et al. “Differential Postsynaptic Compartments in the Laterocapsular Division of the Central Nucleus of Amygdala for Afferents from the Parabrachial Nucleus and the Basolateral Nucleus in the Rat.” Journal of Comparative Neurology, vol. 518, no. 23, Wiley-Blackwell, 2010, pp. 4771–91, doi:10.1002/cne.22487.","apa":"Dong, Y., Fukazawa, Y., Wang, W., Kamasawa, N., & Shigemoto, R. (2010). Differential postsynaptic compartments in the laterocapsular division of the central nucleus of amygdala for afferents from the parabrachial nucleus and the basolateral nucleus in the rat. Journal of Comparative Neurology. Wiley-Blackwell. https://doi.org/10.1002/cne.22487","chicago":"Dong, Yu, Yugo Fukazawa, Wen Wang, Naomi Kamasawa, and Ryuichi Shigemoto. “Differential Postsynaptic Compartments in the Laterocapsular Division of the Central Nucleus of Amygdala for Afferents from the Parabrachial Nucleus and the Basolateral Nucleus in the Rat.” Journal of Comparative Neurology. Wiley-Blackwell, 2010. https://doi.org/10.1002/cne.22487.","ista":"Dong Y, Fukazawa Y, Wang W, Kamasawa N, Shigemoto R. 2010. Differential postsynaptic compartments in the laterocapsular division of the central nucleus of amygdala for afferents from the parabrachial nucleus and the basolateral nucleus in the rat. Journal of Comparative Neurology. 518(23), 4771–4791.","ama":"Dong Y, Fukazawa Y, Wang W, Kamasawa N, Shigemoto R. Differential postsynaptic compartments in the laterocapsular division of the central nucleus of amygdala for afferents from the parabrachial nucleus and the basolateral nucleus in the rat. Journal of Comparative Neurology. 2010;518(23):4771-4791. doi:10.1002/cne.22487"},"doi":"10.1002/cne.22487","extern":1,"status":"public","volume":518,"publist_id":"4391","issue":"23","date_created":"2018-12-11T11:58:05Z","_id":"2510","quality_controlled":0,"publisher":"Wiley-Blackwell","author":[{"full_name":"Dong, Yu-Lin","first_name":"Yu","last_name":"Dong"},{"full_name":"Fukazawa, Yugo","last_name":"Fukazawa","first_name":"Yugo"},{"first_name":"Wen","last_name":"Wang","full_name":"Wang, Wen"},{"full_name":"Kamasawa, Naomi","last_name":"Kamasawa","first_name":"Naomi"},{"first_name":"Ryuichi","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","last_name":"Shigemoto","full_name":"Ryuichi Shigemoto","orcid":"0000-0001-8761-9444"}],"page":"4771 - 4791","year":"2010","publication_status":"published","date_updated":"2021-01-12T06:57:55Z","date_published":"2010-12-01T00:00:00Z"}]