[{"publisher":"BioMed Central","date_published":"2016-09-02T00:00:00Z","file_date_updated":"2020-07-14T12:44:42Z","title":"Steering cell migration by alternating blebs and actin-rich protrusions","publication_status":"published","abstract":[{"lang":"eng","text":"Background: High directional persistence is often assumed to enhance the efficiency of chemotactic migration. Yet, cells in vivo usually display meandering trajectories with relatively low directional persistence, and the control and function of directional persistence during cell migration in three-dimensional environments are poorly understood. Results: Here, we use mesendoderm progenitors migrating during zebrafish gastrulation as a model system to investigate the control of directional persistence during migration in vivo. We show that progenitor cells alternate persistent run phases with tumble phases that result in cell reorientation. Runs are characterized by the formation of directed actin-rich protrusions and tumbles by enhanced blebbing. Increasing the proportion of actin-rich protrusions or blebs leads to longer or shorter run phases, respectively. Importantly, both reducing and increasing run phases result in larger spatial dispersion of the cells, indicative of reduced migration precision. A physical model quantitatively recapitulating the migratory behavior of mesendoderm progenitors indicates that the ratio of tumbling to run times, and thus the specific degree of directional persistence of migration, are critical for optimizing migration precision. Conclusions: Together, our experiments and model provide mechanistic insight into the control of migration directionality for cells moving in three-dimensional environments that combine different protrusion types, whereby the proportion of blebs to actin-rich protrusions determines the directional persistence and precision of movement by regulating the ratio of tumbling to run times."}],"ddc":["572","576"],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"CaHe"}],"date_updated":"2021-01-12T06:49:32Z","citation":{"chicago":"Diz Muñoz, Alba, Pawel Romanczuk, Weimiao Yu, Martin Bergert, Kenzo Ivanovitch, Guillame Salbreux, Carl-Philipp J Heisenberg, and Ewa Paluch. “Steering Cell Migration by Alternating Blebs and Actin-Rich Protrusions.” <i>BMC Biology</i>. BioMed Central, 2016. <a href=\"https://doi.org/10.1186/s12915-016-0294-x\">https://doi.org/10.1186/s12915-016-0294-x</a>.","ista":"Diz Muñoz A, Romanczuk P, Yu W, Bergert M, Ivanovitch K, Salbreux G, Heisenberg C-PJ, Paluch E. 2016. Steering cell migration by alternating blebs and actin-rich protrusions. BMC Biology. 14(1), 74.","ama":"Diz Muñoz A, Romanczuk P, Yu W, et al. Steering cell migration by alternating blebs and actin-rich protrusions. <i>BMC Biology</i>. 2016;14(1). doi:<a href=\"https://doi.org/10.1186/s12915-016-0294-x\">10.1186/s12915-016-0294-x</a>","apa":"Diz Muñoz, A., Romanczuk, P., Yu, W., Bergert, M., Ivanovitch, K., Salbreux, G., … Paluch, E. (2016). Steering cell migration by alternating blebs and actin-rich protrusions. <i>BMC Biology</i>. BioMed Central. <a href=\"https://doi.org/10.1186/s12915-016-0294-x\">https://doi.org/10.1186/s12915-016-0294-x</a>","ieee":"A. Diz Muñoz <i>et al.</i>, “Steering cell migration by alternating blebs and actin-rich protrusions,” <i>BMC Biology</i>, vol. 14, no. 1. BioMed Central, 2016.","short":"A. Diz Muñoz, P. Romanczuk, W. Yu, M. Bergert, K. Ivanovitch, G. Salbreux, C.-P.J. Heisenberg, E. Paluch, BMC Biology 14 (2016).","mla":"Diz Muñoz, Alba, et al. “Steering Cell Migration by Alternating Blebs and Actin-Rich Protrusions.” <i>BMC Biology</i>, vol. 14, no. 1, 74, BioMed Central, 2016, doi:<a href=\"https://doi.org/10.1186/s12915-016-0294-x\">10.1186/s12915-016-0294-x</a>."},"publist_id":"6049","intvolume":"        14","publication":"BMC Biology","scopus_import":1,"acknowledgement":"We thank K. Lee, C. Norden, A. Webb, and the members of the Paluch lab for\r\ncomments on the manuscript. We are grateful to P. Rørth and Peter Dieterich\r\nfor discussions, S. Ares, Y. Arboleda-Estudillo and S. Schneider for technical help,\r\nM. Biro for help with programming, and the BIOTEC/MPI-CBG and IST zebrafish\r\nand imaging facilities for help and advice at various stages of this project. This work was supported by the Max Planck Society, the Medical Research Council UK (core funding to the MRC LMCB), and by grants from the Polish Ministry of Science and Higher Education (454/N-MPG/2009/0) to EKP, the Deutsche Forschungsgemeinschaft (HE 3231/6-1 and PA 1590/1-1) to CPH and EKP, a A*Star JCO career development award (12302FG010) to WY and a Damon Runyon fellowship award to ADM (DRG 2157-12). This work was also supported by the Francis Crick Institute which receives its core funding from Cancer Research UK (FC001317), the UK Medical Research Council (FC001317), and the Wellcome Trust (FC001317) to GS.","year":"2016","doi":"10.1186/s12915-016-0294-x","day":"02","oa":1,"language":[{"iso":"eng"}],"volume":14,"acknowledged_ssus":[{"_id":"LifeSc"}],"month":"09","has_accepted_license":"1","file":[{"file_name":"IST-2016-695-v1+1_s12915-016-0294-x.pdf","date_updated":"2020-07-14T12:44:42Z","date_created":"2018-12-12T10:13:20Z","content_type":"application/pdf","file_size":1875695,"access_level":"open_access","creator":"system","file_id":"5002","checksum":"0bfa484ac69a0a560fb9a4589aeda7f6","relation":"main_file"}],"article_number":"74","issue":"1","author":[{"last_name":"Diz Muñoz","first_name":"Alba","full_name":"Diz Muñoz, Alba"},{"first_name":"Pawel","last_name":"Romanczuk","full_name":"Romanczuk, Pawel"},{"first_name":"Weimiao","last_name":"Yu","full_name":"Yu, Weimiao"},{"last_name":"Bergert","first_name":"Martin","full_name":"Bergert, Martin"},{"last_name":"Ivanovitch","first_name":"Kenzo","full_name":"Ivanovitch, Kenzo"},{"first_name":"Guillame","last_name":"Salbreux","full_name":"Salbreux, Guillame"},{"full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","last_name":"Heisenberg","first_name":"Carl-Philipp J"},{"last_name":"Paluch","first_name":"Ewa","full_name":"Paluch, Ewa"}],"quality_controlled":"1","project":[{"name":"Analysis of the Formation and Function of Different Cell Protusion Types During Cell Migration in Vivo","grant_number":"HE_3231/6-1","_id":"252064B8-B435-11E9-9278-68D0E5697425"}],"oa_version":"Published Version","pubrep_id":"695","status":"public","date_created":"2018-12-11T11:51:04Z","type":"journal_article","_id":"1271"},{"status":"public","date_created":"2018-12-11T11:51:04Z","type":"journal_article","_id":"1272","pubrep_id":"694","author":[{"full_name":"Held, Martin","first_name":"Martin","last_name":"Held"},{"full_name":"Huber, Stefan","id":"4700A070-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8871-5814","first_name":"Stefan","last_name":"Huber"},{"first_name":"Peter","last_name":"Palfrader","full_name":"Palfrader, Peter"}],"quality_controlled":"1","oa_version":"Published Version","language":[{"iso":"eng"}],"volume":13,"has_accepted_license":"1","month":"09","file":[{"access_level":"open_access","file_size":1678369,"file_id":"5206","checksum":"c746f3a48edb62b588d92ea5d0fd2c0e","creator":"system","relation":"main_file","file_name":"IST-2016-694-v1+1_Generalized_offsetting_of_planar_structures_using_skeletons.pdf","date_created":"2018-12-12T10:16:20Z","date_updated":"2020-07-14T12:44:42Z","content_type":"application/pdf"}],"issue":"5","acknowledgement":"This work was supported by Austrian Science Fund (FWF): P25816-N15.","doi":"10.1080/16864360.2016.1150718","page":"712 - 721","year":"2016","day":"02","oa":1,"publist_id":"6048","intvolume":"        13","publication":"Computer-Aided Design and Applications","scopus_import":1,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)"},"user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"HeEd"}],"date_updated":"2021-01-12T06:49:32Z","citation":{"ista":"Held M, Huber S, Palfrader P. 2016. Generalized offsetting of planar structures using skeletons. Computer-Aided Design and Applications. 13(5), 712–721.","chicago":"Held, Martin, Stefan Huber, and Peter Palfrader. “Generalized Offsetting of Planar Structures Using Skeletons.” <i>Computer-Aided Design and Applications</i>. Taylor and Francis, 2016. <a href=\"https://doi.org/10.1080/16864360.2016.1150718\">https://doi.org/10.1080/16864360.2016.1150718</a>.","ama":"Held M, Huber S, Palfrader P. Generalized offsetting of planar structures using skeletons. <i>Computer-Aided Design and Applications</i>. 2016;13(5):712-721. doi:<a href=\"https://doi.org/10.1080/16864360.2016.1150718\">10.1080/16864360.2016.1150718</a>","apa":"Held, M., Huber, S., &#38; Palfrader, P. (2016). Generalized offsetting of planar structures using skeletons. <i>Computer-Aided Design and Applications</i>. Taylor and Francis. <a href=\"https://doi.org/10.1080/16864360.2016.1150718\">https://doi.org/10.1080/16864360.2016.1150718</a>","ieee":"M. Held, S. Huber, and P. Palfrader, “Generalized offsetting of planar structures using skeletons,” <i>Computer-Aided Design and Applications</i>, vol. 13, no. 5. Taylor and Francis, pp. 712–721, 2016.","mla":"Held, Martin, et al. “Generalized Offsetting of Planar Structures Using Skeletons.” <i>Computer-Aided Design and Applications</i>, vol. 13, no. 5, Taylor and Francis, 2016, pp. 712–21, doi:<a href=\"https://doi.org/10.1080/16864360.2016.1150718\">10.1080/16864360.2016.1150718</a>.","short":"M. Held, S. Huber, P. Palfrader, Computer-Aided Design and Applications 13 (2016) 712–721."},"file_date_updated":"2020-07-14T12:44:42Z","publisher":"Taylor and Francis","date_published":"2016-09-02T00:00:00Z","title":"Generalized offsetting of planar structures using skeletons","publication_status":"published","abstract":[{"text":"We study different means to extend offsetting based on skeletal structures beyond the well-known constant-radius and mitered offsets supported by Voronoi diagrams and straight skeletons, for which the orthogonal distance of offset elements to their respective input elements is constant and uniform over all input elements. Our main contribution is a new geometric structure, called variable-radius Voronoi diagram, which supports the computation of variable-radius offsets, i.e., offsets whose distance to the input is allowed to vary along the input. We discuss properties of this structure and sketch a prototype implementation that supports the computation of variable-radius offsets based on this new variant of Voronoi diagrams.","lang":"eng"}],"ddc":["004","516"]},{"status":"public","main_file_link":[{"url":"https://hal.archives-ouvertes.fr/hal-01595056/","open_access":"1"}],"date_created":"2018-12-11T11:51:04Z","_id":"1273","type":"journal_article","author":[{"last_name":"Porco","first_name":"Silvana","full_name":"Porco, Silvana"},{"full_name":"Larrieu, Antoine","last_name":"Larrieu","first_name":"Antoine"},{"first_name":"Yujuan","last_name":"Du","full_name":"Du, Yujuan"},{"first_name":"Allison","last_name":"Gaudinier","full_name":"Gaudinier, Allison"},{"last_name":"Goh","first_name":"Tatsuaki","full_name":"Goh, Tatsuaki"},{"first_name":"Kamal","last_name":"Swarup","full_name":"Swarup, Kamal"},{"first_name":"Ranjan","last_name":"Swarup","full_name":"Swarup, Ranjan"},{"full_name":"Kuempers, Britta","last_name":"Kuempers","first_name":"Britta"},{"full_name":"Bishopp, Anthony","last_name":"Bishopp","first_name":"Anthony"},{"first_name":"Julien","last_name":"Lavenus","full_name":"Lavenus, Julien"},{"full_name":"Casimiro, Ilda","last_name":"Casimiro","first_name":"Ilda"},{"full_name":"Hill, Kristine","last_name":"Hill","first_name":"Kristine"},{"orcid":"0000-0002-8510-9739","last_name":"Benková","first_name":"Eva","full_name":"Benková, Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Fukaki, Hidehiro","first_name":"Hidehiro","last_name":"Fukaki"},{"full_name":"Brady, Siobhan","first_name":"Siobhan","last_name":"Brady"},{"first_name":"Ben","last_name":"Scheres","full_name":"Scheres, Ben"},{"first_name":"Benjamin","last_name":"Peéet","full_name":"Peéet, Benjamin"},{"full_name":"Bennett, Malcolm","last_name":"Bennett","first_name":"Malcolm"}],"quality_controlled":"1","oa_version":"Preprint","language":[{"iso":"eng"}],"volume":143,"month":"09","issue":"18","acknowledgement":"We acknowledge the support of glasshouse technicians at the University of\r\nNottingham for help with plant growth and the Nottingham\r\nArabidopsis\r\nStock Centre\r\n(NASC) for providing\r\nArabidopsis\r\nlines. This research was supported by the Biotechnology and Biological Sciences Research Council (BBSRC) (to A.B. and M.J.B.); the European Research Council (ERC) Advanced Grant SysArc (to B.S.) and FUTUREROOTS (to M.J.B.); The Royal Society for University and Wolfson Research Fellowship awards (to A.B. and M.J.B.); a Federation of European Biochemical Societies (FEBS) Long-Term Fellowship (to B.P.); an Intra-European Fellowship for Career Development under the 7th framework of the European Commission [IEF-2008-220506 to B.P.]; a European Molecular Biology Organization (EMBO) Long-Term Fellowship (to B.P.); and a European Reintegration Grant under the 7th framework of the European Commission [ERG-2010-276662 to B.P.]; Interuniversity Attraction Poles Programme [initiated by the Belgian Science Policy Office (Federaal Wetenschapsbeleid)] (to M.J.B.); The Ministry of Education, Culture, Sports, Science, and Technology (MEXT), Japan: Grants-in-Aid for Scientific Research on Innovative Areas [25110330 to H.F.] and a JSPS Research Fellowship for Young Scientists [12J02079 to T.G.]; funds for research performed by S.M.B. and A.G. were provided by University of California, Davis startup funds.","day":"13","year":"2016","page":"3340 - 3349","doi":"10.1242/dev.136283","oa":1,"intvolume":"       143","publist_id":"6044","publication":"Development","scopus_import":1,"user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","date_updated":"2021-01-12T06:49:32Z","department":[{"_id":"EvBe"}],"citation":{"mla":"Porco, Silvana, et al. “Lateral Root Emergence in Arabidopsis Is Dependent on Transcription Factor LBD29 Regulation of Auxin Influx Carrier LAX3.” <i>Development</i>, vol. 143, no. 18, Company of Biologists, 2016, pp. 3340–49, doi:<a href=\"https://doi.org/10.1242/dev.136283\">10.1242/dev.136283</a>.","short":"S. Porco, A. Larrieu, Y. Du, A. Gaudinier, T. Goh, K. Swarup, R. Swarup, B. Kuempers, A. Bishopp, J. Lavenus, I. Casimiro, K. Hill, E. Benková, H. Fukaki, S. Brady, B. Scheres, B. Peéet, M. Bennett, Development 143 (2016) 3340–3349.","ista":"Porco S, Larrieu A, Du Y, Gaudinier A, Goh T, Swarup K, Swarup R, Kuempers B, Bishopp A, Lavenus J, Casimiro I, Hill K, Benková E, Fukaki H, Brady S, Scheres B, Peéet B, Bennett M. 2016. Lateral root emergence in Arabidopsis is dependent on transcription factor LBD29 regulation of auxin influx carrier LAX3. Development. 143(18), 3340–3349.","chicago":"Porco, Silvana, Antoine Larrieu, Yujuan Du, Allison Gaudinier, Tatsuaki Goh, Kamal Swarup, Ranjan Swarup, et al. “Lateral Root Emergence in Arabidopsis Is Dependent on Transcription Factor LBD29 Regulation of Auxin Influx Carrier LAX3.” <i>Development</i>. Company of Biologists, 2016. <a href=\"https://doi.org/10.1242/dev.136283\">https://doi.org/10.1242/dev.136283</a>.","ama":"Porco S, Larrieu A, Du Y, et al. Lateral root emergence in Arabidopsis is dependent on transcription factor LBD29 regulation of auxin influx carrier LAX3. <i>Development</i>. 2016;143(18):3340-3349. doi:<a href=\"https://doi.org/10.1242/dev.136283\">10.1242/dev.136283</a>","apa":"Porco, S., Larrieu, A., Du, Y., Gaudinier, A., Goh, T., Swarup, K., … Bennett, M. (2016). Lateral root emergence in Arabidopsis is dependent on transcription factor LBD29 regulation of auxin influx carrier LAX3. <i>Development</i>. Company of Biologists. <a href=\"https://doi.org/10.1242/dev.136283\">https://doi.org/10.1242/dev.136283</a>","ieee":"S. Porco <i>et al.</i>, “Lateral root emergence in Arabidopsis is dependent on transcription factor LBD29 regulation of auxin influx carrier LAX3,” <i>Development</i>, vol. 143, no. 18. Company of Biologists, pp. 3340–3349, 2016."},"date_published":"2016-09-13T00:00:00Z","publisher":"Company of Biologists","title":"Lateral root emergence in Arabidopsis is dependent on transcription factor LBD29 regulation of auxin influx carrier LAX3","abstract":[{"text":"Lateral root primordia (LRP) originate from pericycle stem cells located deep within parental root tissues. LRP emerge through overlying root tissues by inducing auxin-dependent cell separation and hydraulic changes in adjacent cells. The auxin-inducible auxin influx carrier LAX3 plays a key role concentrating this signal in cells overlying LRP. Delimiting LAX3 expression to two adjacent cell files overlying new LRP is crucial to ensure that auxin-regulated cell separation occurs solely along their shared walls. Multiscale modeling has predicted that this highly focused pattern of expression requires auxin to sequentially induce auxin efflux and influx carriers PIN3 and LAX3, respectively. Consistent with model predictions, we report that auxin-inducible LAX3 expression is regulated indirectly by AUXIN RESPONSE FACTOR 7 (ARF7). Yeast one-hybrid screens revealed that the LAX3 promoter is bound by the transcription factor LBD29, which is a direct target for regulation by ARF7. Disrupting auxin-inducible LBD29 expression or expressing an LBD29-SRDX transcriptional repressor phenocopied the lax3 mutant, resulting in delayed lateral root emergence. We conclude that sequential LBD29 and LAX3 induction by auxin is required to coordinate cell separation and organ emergence.","lang":"eng"}],"publication_status":"published"},{"pubrep_id":"692","type":"journal_article","_id":"1274","date_created":"2018-12-11T11:51:05Z","status":"public","article_number":"33754","file":[{"access_level":"open_access","file_size":2895147,"file_id":"5008","checksum":"ee371fbc9124ad93157a95829264e4fe","creator":"system","relation":"main_file","file_name":"IST-2016-692-v1+1_srep33754.pdf","date_created":"2018-12-12T10:13:25Z","date_updated":"2020-07-14T12:44:42Z","content_type":"application/pdf"}],"month":"09","has_accepted_license":"1","article_processing_charge":"No","volume":6,"language":[{"iso":"eng"}],"oa_version":"Published Version","quality_controlled":"1","author":[{"last_name":"Mazur","first_name":"Ewa","full_name":"Mazur, Ewa"},{"last_name":"Benková","first_name":"Eva","orcid":"0000-0002-8510-9739","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","full_name":"Benková, Eva"},{"first_name":"Jirí","last_name":"Friml","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí"}],"related_material":{"record":[{"status":"public","id":"545","relation":"later_version"}]},"scopus_import":"1","publication":"Scientific Reports","intvolume":"         6","publist_id":"6042","oa":1,"day":"21","year":"2016","doi":"10.1038/srep33754","acknowledgement":"We wish to thank Prof. Ewa U. Kurczyńska for initiation of this work and valuable advices. We thank Martine De Cock for help in preparing the manuscript. This work was supported by the European Research Council (project ERC-2011-StG-20101109-PSDP), the European Social Fund (CZ.1.07/2.3.00/20.0043), and the Czech Science Foundation GAČR (GA13-40637 S) to J.F., (GA 13-39982S) to E.B. and E.M. and in part by the European Regional Development Fund (project “CEITEC, Central European Institute of Technology”, CZ.1.05/1.1.00/02.0068).","ddc":["581"],"abstract":[{"text":"Synchronized tissue polarization during regeneration or de novo vascular tissue formation is a plant-specific example of intercellular communication and coordinated development. According to the canalization hypothesis, the plant hormone auxin serves as polarizing signal that mediates directional channel formation underlying the spatio-temporal vasculature patterning. A necessary part of canalization is a positive feedback between auxin signaling and polarity of the intercellular auxin flow. The cellular and molecular mechanisms of this process are still poorly understood, not the least, because of a lack of a suitable model system. We show that the main genetic model plant, Arabidopsis (Arabidopsis thaliana) can be used to study the canalization during vascular cambium regeneration and new vasculature formation. We monitored localized auxin responses, directional auxin-transport channels formation, and establishment of new vascular cambium polarity during regenerative processes after stem wounding. The increased auxin response above and around the wound preceded the formation of PIN1 auxin transporter-marked channels from the primarily homogenous tissue and the transient, gradual changes in PIN1 localization preceded the polarity of newly formed vascular tissue. Thus, Arabidopsis is a useful model for studies of coordinated tissue polarization and vasculature formation after wounding allowing for genetic and mechanistic dissection of the canalization hypothesis.","lang":"eng"}],"publication_status":"published","title":"Vascular cambium regeneration and vessel formation in wounded inflorescence stems of Arabidopsis","publisher":"Nature Publishing Group","file_date_updated":"2020-07-14T12:44:42Z","date_published":"2016-09-21T00:00:00Z","pmid":1,"citation":{"ista":"Mazur E, Benková E, Friml J. 2016. Vascular cambium regeneration and vessel formation in wounded inflorescence stems of Arabidopsis. Scientific Reports. 6, 33754.","chicago":"Mazur, Ewa, Eva Benková, and Jiří Friml. “Vascular Cambium Regeneration and Vessel Formation in Wounded Inflorescence Stems of Arabidopsis.” <i>Scientific Reports</i>. Nature Publishing Group, 2016. <a href=\"https://doi.org/10.1038/srep33754\">https://doi.org/10.1038/srep33754</a>.","ama":"Mazur E, Benková E, Friml J. Vascular cambium regeneration and vessel formation in wounded inflorescence stems of Arabidopsis. <i>Scientific Reports</i>. 2016;6. doi:<a href=\"https://doi.org/10.1038/srep33754\">10.1038/srep33754</a>","apa":"Mazur, E., Benková, E., &#38; Friml, J. (2016). Vascular cambium regeneration and vessel formation in wounded inflorescence stems of Arabidopsis. <i>Scientific Reports</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/srep33754\">https://doi.org/10.1038/srep33754</a>","ieee":"E. Mazur, E. Benková, and J. Friml, “Vascular cambium regeneration and vessel formation in wounded inflorescence stems of Arabidopsis,” <i>Scientific Reports</i>, vol. 6. Nature Publishing Group, 2016.","short":"E. Mazur, E. Benková, J. Friml, Scientific Reports 6 (2016).","mla":"Mazur, Ewa, et al. “Vascular Cambium Regeneration and Vessel Formation in Wounded Inflorescence Stems of Arabidopsis.” <i>Scientific Reports</i>, vol. 6, 33754, Nature Publishing Group, 2016, doi:<a href=\"https://doi.org/10.1038/srep33754\">10.1038/srep33754</a>."},"date_updated":"2025-05-07T11:12:28Z","department":[{"_id":"EvBe"},{"_id":"JiFr"}],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","external_id":{"pmid":["27649687"]}},{"user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","author":[{"full_name":"Callan Jones, Andrew","first_name":"Andrew","last_name":"Callan Jones"},{"orcid":"0000-0003-4088-8633","last_name":"Ruprecht","first_name":"Verena","full_name":"Ruprecht, Verena","id":"4D71A03A-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Wieser, Stefan","id":"355AA5A0-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-2670-2217","first_name":"Stefan","last_name":"Wieser"},{"first_name":"Carl-Philipp J","last_name":"Heisenberg","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J"},{"full_name":"Voituriez, Raphaël","last_name":"Voituriez","first_name":"Raphaël"}],"oa_version":"None","citation":{"mla":"Callan Jones, Andrew, et al. “Callan-Jones et Al. Reply.” <i>Physical Review Letters</i>, vol. 117, no. 13, 139802, American Physical Society, 2016, doi:<a href=\"https://doi.org/10.1103/PhysRevLett.117.139802\">10.1103/PhysRevLett.117.139802</a>.","short":"A. Callan Jones, V. Ruprecht, S. Wieser, C.-P.J. Heisenberg, R. Voituriez, Physical Review Letters 117 (2016).","apa":"Callan Jones, A., Ruprecht, V., Wieser, S., Heisenberg, C.-P. J., &#38; Voituriez, R. (2016). Callan-Jones et al. Reply. <i>Physical Review Letters</i>. American Physical Society. <a href=\"https://doi.org/10.1103/PhysRevLett.117.139802\">https://doi.org/10.1103/PhysRevLett.117.139802</a>","chicago":"Callan Jones, Andrew, Verena Ruprecht, Stefan Wieser, Carl-Philipp J Heisenberg, and Raphaël Voituriez. “Callan-Jones et Al. Reply.” <i>Physical Review Letters</i>. American Physical Society, 2016. <a href=\"https://doi.org/10.1103/PhysRevLett.117.139802\">https://doi.org/10.1103/PhysRevLett.117.139802</a>.","ista":"Callan Jones A, Ruprecht V, Wieser S, Heisenberg C-PJ, Voituriez R. 2016. Callan-Jones et al. Reply. Physical Review Letters. 117(13), 139802.","ama":"Callan Jones A, Ruprecht V, Wieser S, Heisenberg C-PJ, Voituriez R. Callan-Jones et al. Reply. <i>Physical Review Letters</i>. 2016;117(13). doi:<a href=\"https://doi.org/10.1103/PhysRevLett.117.139802\">10.1103/PhysRevLett.117.139802</a>","ieee":"A. Callan Jones, V. Ruprecht, S. Wieser, C.-P. J. Heisenberg, and R. Voituriez, “Callan-Jones et al. Reply,” <i>Physical Review Letters</i>, vol. 117, no. 13. American Physical Society, 2016."},"department":[{"_id":"CaHe"}],"quality_controlled":"1","date_updated":"2021-01-12T06:49:33Z","volume":117,"title":"Callan-Jones et al. Reply","date_published":"2016-09-22T00:00:00Z","language":[{"iso":"eng"}],"publisher":"American Physical Society","article_number":"139802","issue":"13","publication_status":"published","month":"09","status":"public","doi":"10.1103/PhysRevLett.117.139802","year":"2016","day":"22","_id":"1275","type":"journal_article","date_created":"2018-12-11T11:51:05Z","publication":"Physical Review Letters","publist_id":"6041","intvolume":"       117","scopus_import":1},{"scopus_import":1,"publication":"Scientific Reports","publist_id":"6040","intvolume":"         6","oa":1,"doi":"10.1038/srep33607","year":"2016","day":"26","acknowledgement":"We wish to thank CSC – IT Centre for Science (Espoo, Finland) for computational resources. For financial support, we wish to thank the Academy of Finland (TR, IV and PAP; Center of Excellence in Biomembrane Research (IV, TR)), the Finnish Doctoral Programme in Computational Sciences (KK), the Sigrid Juselius Foundation (IV), the Paulo Foundation (PAP), and the European Research Council (IV, TR; Advanced Grant project CROWDED-PRO-LIPIDS). AO acknowledges The Wellcome Trust International Senior Research Fellowship.","ddc":["576"],"publication_status":"published","abstract":[{"lang":"eng","text":"The cytochrome (cyt) bc 1 complex is an integral component of the respiratory electron transfer chain sustaining the energy needs of organisms ranging from humans to bacteria. Due to its ubiquitous role in the energy metabolism, both the oxidation and reduction of the enzyme's substrate co-enzyme Q has been studied vigorously. Here, this vast amount of data is reassessed after probing the substrate reduction steps at the Q i-site of the cyt bc 1 complex of Rhodobacter capsulatus using atomistic molecular dynamics simulations. The simulations suggest that the Lys251 side chain could rotate into the Q i-site to facilitate binding of half-protonated semiquinone-a reaction intermediate that is potentially formed during substrate reduction. At this bent pose, the Lys251 forms a salt bridge with the Asp252, thus making direct proton transfer possible. In the neutral state, the lysine side chain stays close to the conserved binding location of cardiolipin (CL). This back-and-forth motion between the CL and Asp252 indicates that Lys251 functions as a proton shuttle controlled by pH-dependent negative feedback. The CL/K/D switching, which represents a refinement to the previously described CL/K pathway, fine-tunes the proton transfer process. Lastly, the simulation data was used to formulate a mechanism for reducing the substrate at the Q i-site."}],"title":"Atomistic determinants of co-enzyme Q reduction at the Qi-site of the cytochrome bc1 complex","file_date_updated":"2020-07-14T12:44:42Z","date_published":"2016-09-26T00:00:00Z","publisher":"Nature Publishing Group","citation":{"ieee":"P. Postila <i>et al.</i>, “Atomistic determinants of co-enzyme Q reduction at the Qi-site of the cytochrome bc1 complex,” <i>Scientific Reports</i>, vol. 6. Nature Publishing Group, 2016.","ista":"Postila P, Kaszuba K, Kuleta P, Vattulainen I, Sarewicz M, Osyczka A, Róg T. 2016. Atomistic determinants of co-enzyme Q reduction at the Qi-site of the cytochrome bc1 complex. Scientific Reports. 6, 33607.","ama":"Postila P, Kaszuba K, Kuleta P, et al. Atomistic determinants of co-enzyme Q reduction at the Qi-site of the cytochrome bc1 complex. <i>Scientific Reports</i>. 2016;6. doi:<a href=\"https://doi.org/10.1038/srep33607\">10.1038/srep33607</a>","chicago":"Postila, Pekka, Karol Kaszuba, Patryk Kuleta, Ilpo Vattulainen, Marcin Sarewicz, Artur Osyczka, and Tomasz Róg. “Atomistic Determinants of Co-Enzyme Q Reduction at the Qi-Site of the Cytochrome Bc1 Complex.” <i>Scientific Reports</i>. Nature Publishing Group, 2016. <a href=\"https://doi.org/10.1038/srep33607\">https://doi.org/10.1038/srep33607</a>.","apa":"Postila, P., Kaszuba, K., Kuleta, P., Vattulainen, I., Sarewicz, M., Osyczka, A., &#38; Róg, T. (2016). Atomistic determinants of co-enzyme Q reduction at the Qi-site of the cytochrome bc1 complex. <i>Scientific Reports</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/srep33607\">https://doi.org/10.1038/srep33607</a>","mla":"Postila, Pekka, et al. “Atomistic Determinants of Co-Enzyme Q Reduction at the Qi-Site of the Cytochrome Bc1 Complex.” <i>Scientific Reports</i>, vol. 6, 33607, Nature Publishing Group, 2016, doi:<a href=\"https://doi.org/10.1038/srep33607\">10.1038/srep33607</a>.","short":"P. Postila, K. Kaszuba, P. Kuleta, I. Vattulainen, M. Sarewicz, A. Osyczka, T. Róg, Scientific Reports 6 (2016)."},"department":[{"_id":"LeSa"}],"date_updated":"2021-01-12T06:49:34Z","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"pubrep_id":"691","_id":"1276","type":"journal_article","date_created":"2018-12-11T11:51:05Z","status":"public","article_number":"33607","file":[{"creator":"system","checksum":"07c591c1250ebef266333cbc3228b4dd","file_id":"5261","file_size":1960563,"access_level":"open_access","relation":"main_file","file_name":"IST-2016-691-v1+1_srep33607.pdf","content_type":"application/pdf","date_updated":"2020-07-14T12:44:42Z","date_created":"2018-12-12T10:17:09Z"}],"month":"09","has_accepted_license":"1","volume":6,"language":[{"iso":"eng"}],"oa_version":"Published Version","quality_controlled":"1","author":[{"full_name":"Postila, Pekka","last_name":"Postila","first_name":"Pekka"},{"full_name":"Kaszuba, Karol","id":"3FDF9472-F248-11E8-B48F-1D18A9856A87","last_name":"Kaszuba","first_name":"Karol"},{"full_name":"Kuleta, Patryk","first_name":"Patryk","last_name":"Kuleta"},{"last_name":"Vattulainen","first_name":"Ilpo","full_name":"Vattulainen, Ilpo"},{"last_name":"Sarewicz","first_name":"Marcin","full_name":"Sarewicz, Marcin"},{"full_name":"Osyczka, Artur","first_name":"Artur","last_name":"Osyczka"},{"full_name":"Róg, Tomasz","first_name":"Tomasz","last_name":"Róg"}]},{"volume":113,"language":[{"iso":"eng"}],"issue":"39","month":"09","author":[{"full_name":"Ortiz Morea, Fausto","last_name":"Ortiz Morea","first_name":"Fausto"},{"last_name":"Savatin","first_name":"Daniel","full_name":"Savatin, Daniel"},{"full_name":"Dejonghe, Wim","last_name":"Dejonghe","first_name":"Wim"},{"full_name":"Kumar, Rahul","last_name":"Kumar","first_name":"Rahul"},{"last_name":"Luo","first_name":"Yu","full_name":"Luo, Yu"},{"first_name":"Maciek","last_name":"Adamowski","orcid":"0000-0001-6463-5257","id":"45F536D2-F248-11E8-B48F-1D18A9856A87","full_name":"Adamowski, Maciek"},{"full_name":"Van Begin, Jos","last_name":"Van Begin","first_name":"Jos"},{"full_name":"Dressano, Keini","last_name":"Dressano","first_name":"Keini"},{"full_name":"De Oliveira, Guilherme","first_name":"Guilherme","last_name":"De Oliveira"},{"full_name":"Zhao, Xiuyang","last_name":"Zhao","first_name":"Xiuyang"},{"full_name":"Lu, Qing","last_name":"Lu","first_name":"Qing"},{"full_name":"Madder, Annemieke","last_name":"Madder","first_name":"Annemieke"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí","last_name":"Friml","first_name":"Jirí","orcid":"0000-0002-8302-7596"},{"full_name":"De Moura, Daniel","last_name":"De Moura","first_name":"Daniel"},{"last_name":"Russinova","first_name":"Eugenia","full_name":"Russinova, Eugenia"}],"oa_version":"Preprint","quality_controlled":"1","main_file_link":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5047203/","open_access":"1"}],"status":"public","type":"journal_article","_id":"1277","date_created":"2018-12-11T11:51:06Z","title":"Danger-associated peptide signaling in Arabidopsis requires clathrin","publisher":"National Academy of Sciences","date_published":"2016-09-27T00:00:00Z","abstract":[{"lang":"eng","text":"The Arabidopsis thaliana endogenous elicitor peptides (AtPeps) are released into the apoplast after cellular damage caused by pathogens or wounding to induce innate immunity by direct binding to the membrane-localized leucine-rich repeat receptor kinases, PEP RECEPTOR1 (PEPR1) and PEPR2. Although the PEPR-mediated signaling components and responses have been studied extensively, the contributions of the subcellular localization and dynamics of the active PEPRs remain largely unknown. We used live-cell imaging of the fluorescently labeled and bioactive pep1 to visualize the intracellular behavior of the PEPRs in the Arabidopsis root meristem. We found that AtPep1 decorated the plasma membrane (PM) in a receptor-dependent manner and cointernalized with PEPRs. Trafficking of the AtPep1-PEPR1 complexes to the vacuole required neither the trans-Golgi network/early endosome (TGN/EE)-localized vacuolar H+ -ATPase activity nor the function of the brefeldin A-sensitive ADP-ribosylation factor-guanine exchange factors (ARF-GEFs). In addition, AtPep1 and different TGN/EE markers colocalized only rarely, implying that the intracellular route of this receptor-ligand pair is largely independent of the TGN/EE. Inducible overexpression of the Arabidopsis clathrin coat disassembly factor, Auxilin2, which inhibits clathrin-mediated endocytosis (CME), impaired the AtPep1-PEPR1 internalization and compromised AtPep1-mediated responses. Our results show that clathrin function at the PM is required to induce plant defense responses, likely through CME of cell surface-located signaling components.\r\n"}],"publication_status":"published","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","citation":{"ieee":"F. Ortiz Morea <i>et al.</i>, “Danger-associated peptide signaling in Arabidopsis requires clathrin,” <i>PNAS</i>, vol. 113, no. 39. National Academy of Sciences, pp. 11028–11033, 2016.","ama":"Ortiz Morea F, Savatin D, Dejonghe W, et al. Danger-associated peptide signaling in Arabidopsis requires clathrin. <i>PNAS</i>. 2016;113(39):11028-11033. doi:<a href=\"https://doi.org/10.1073/pnas.1605588113\">10.1073/pnas.1605588113</a>","ista":"Ortiz Morea F, Savatin D, Dejonghe W, Kumar R, Luo Y, Adamowski M, Van Begin J, Dressano K, De Oliveira G, Zhao X, Lu Q, Madder A, Friml J, De Moura D, Russinova E. 2016. Danger-associated peptide signaling in Arabidopsis requires clathrin. PNAS. 113(39), 11028–11033.","chicago":"Ortiz Morea, Fausto, Daniel Savatin, Wim Dejonghe, Rahul Kumar, Yu Luo, Maciek Adamowski, Jos Van Begin, et al. “Danger-Associated Peptide Signaling in Arabidopsis Requires Clathrin.” <i>PNAS</i>. National Academy of Sciences, 2016. <a href=\"https://doi.org/10.1073/pnas.1605588113\">https://doi.org/10.1073/pnas.1605588113</a>.","apa":"Ortiz Morea, F., Savatin, D., Dejonghe, W., Kumar, R., Luo, Y., Adamowski, M., … Russinova, E. (2016). Danger-associated peptide signaling in Arabidopsis requires clathrin. <i>PNAS</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.1605588113\">https://doi.org/10.1073/pnas.1605588113</a>","short":"F. Ortiz Morea, D. Savatin, W. Dejonghe, R. Kumar, Y. Luo, M. Adamowski, J. Van Begin, K. Dressano, G. De Oliveira, X. Zhao, Q. Lu, A. Madder, J. Friml, D. De Moura, E. Russinova, PNAS 113 (2016) 11028–11033.","mla":"Ortiz Morea, Fausto, et al. “Danger-Associated Peptide Signaling in Arabidopsis Requires Clathrin.” <i>PNAS</i>, vol. 113, no. 39, National Academy of Sciences, 2016, pp. 11028–33, doi:<a href=\"https://doi.org/10.1073/pnas.1605588113\">10.1073/pnas.1605588113</a>."},"date_updated":"2021-01-12T06:49:34Z","department":[{"_id":"JiFr"}],"publication":"PNAS","intvolume":"       113","publist_id":"6039","scopus_import":1,"day":"27","page":"11028 - 11033","year":"2016","doi":"10.1073/pnas.1605588113","acknowledgement":"F.A.O.-M. was supported by special\r\nresearch funding from the Flemish Government for a joint doctorate fellowship\r\nat Ghent University, and funding from the Student Program\r\n–\r\nGraduate Studies\r\nPlan Program from the Coordination for the Improvement of Higher Educa-\r\ntion Personnel, Brazil, for a doctorate fellowship at the University of São Paulo.\r\nX.Z. and Q.L. are indebted to the China Science Council and G.P.d.O. to the\r\n“\r\nCiência sem Fronteiras\r\n”\r\nfor predoctoral fellowships. R.K. and Y.L. have re-\r\nceived postdoctoral fellowships from the Belgian Science Policy Office. This\r\nresearch was supported by Flanders Research Foundation Grant G008416N\r\n(to E.R.) and by the São Paulo Research Foundation and the National Council\r\nfor Scientific and Technological Development (CNPq) (D.S.d.M.). D.S.d.M. is a\r\nresearch fellow of CNPq.\r\nWe thank D. Van Damme, E. Mylle, M. Castro Silva-Filho,\r\nand J. Goeman for providing usefu\r\nl advice and technical assistance;\r\nI. Hara-Nishimura, J. Lin, G. Jürgens, M. A. Johnson, and P. Bozhkov for sharing\r\npublished materials; and M. Nowack and M. Fendrych for kindly donating the\r\npUBQ10::ATG8-YFP\r\n-expressing marker line.","oa":1},{"author":[{"first_name":"Hitomi","last_name":"Matsuno","full_name":"Matsuno, Hitomi"},{"full_name":"Kudoh, Moeko","first_name":"Moeko","last_name":"Kudoh"},{"full_name":"Watakabe, Akiya","last_name":"Watakabe","first_name":"Akiya"},{"full_name":"Yamamori, Tetsuo","first_name":"Tetsuo","last_name":"Yamamori"},{"full_name":"Shigemoto, Ryuichi","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8761-9444","first_name":"Ryuichi","last_name":"Shigemoto"},{"last_name":"Nagao","first_name":"Soichi","full_name":"Nagao, Soichi"}],"quality_controlled":"1","oa_version":"Published Version","language":[{"iso":"eng"}],"article_processing_charge":"No","volume":11,"has_accepted_license":"1","month":"10","issue":"10","file":[{"file_name":"IST-2016-689-v1+1_journal.pone.0164037.PDF","content_type":"application/pdf","date_updated":"2020-07-14T12:44:42Z","date_created":"2018-12-12T10:17:16Z","creator":"system","file_id":"5269","checksum":"7c0ba0ca6d79844059158059d2a38d25","file_size":3657084,"access_level":"open_access","relation":"main_file"}],"article_number":"e0164037","status":"public","date_created":"2018-12-11T11:51:06Z","_id":"1278","type":"journal_article","article_type":"original","pubrep_id":"689","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"date_updated":"2021-01-12T06:49:34Z","department":[{"_id":"RySh"}],"citation":{"ieee":"H. Matsuno, M. Kudoh, A. Watakabe, T. Yamamori, R. Shigemoto, and S. Nagao, “Distribution and structure of synapses on medial vestibular nuclear neurons targeted by cerebellar flocculus purkinje cells and vestibular nerve in mice: Light and electron microscopy studies,” <i>PLoS One</i>, vol. 11, no. 10. Public Library of Science, 2016.","ista":"Matsuno H, Kudoh M, Watakabe A, Yamamori T, Shigemoto R, Nagao S. 2016. Distribution and structure of synapses on medial vestibular nuclear neurons targeted by cerebellar flocculus purkinje cells and vestibular nerve in mice: Light and electron microscopy studies. PLoS One. 11(10), e0164037.","ama":"Matsuno H, Kudoh M, Watakabe A, Yamamori T, Shigemoto R, Nagao S. Distribution and structure of synapses on medial vestibular nuclear neurons targeted by cerebellar flocculus purkinje cells and vestibular nerve in mice: Light and electron microscopy studies. <i>PLoS One</i>. 2016;11(10). doi:<a href=\"https://doi.org/10.1371/journal.pone.0164037\">10.1371/journal.pone.0164037</a>","chicago":"Matsuno, Hitomi, Moeko Kudoh, Akiya Watakabe, Tetsuo Yamamori, Ryuichi Shigemoto, and Soichi Nagao. “Distribution and Structure of Synapses on Medial Vestibular Nuclear Neurons Targeted by Cerebellar Flocculus Purkinje Cells and Vestibular Nerve in Mice: Light and Electron Microscopy Studies.” <i>PLoS One</i>. Public Library of Science, 2016. <a href=\"https://doi.org/10.1371/journal.pone.0164037\">https://doi.org/10.1371/journal.pone.0164037</a>.","apa":"Matsuno, H., Kudoh, M., Watakabe, A., Yamamori, T., Shigemoto, R., &#38; Nagao, S. (2016). Distribution and structure of synapses on medial vestibular nuclear neurons targeted by cerebellar flocculus purkinje cells and vestibular nerve in mice: Light and electron microscopy studies. <i>PLoS One</i>. Public Library of Science. <a href=\"https://doi.org/10.1371/journal.pone.0164037\">https://doi.org/10.1371/journal.pone.0164037</a>","mla":"Matsuno, Hitomi, et al. “Distribution and Structure of Synapses on Medial Vestibular Nuclear Neurons Targeted by Cerebellar Flocculus Purkinje Cells and Vestibular Nerve in Mice: Light and Electron Microscopy Studies.” <i>PLoS One</i>, vol. 11, no. 10, e0164037, Public Library of Science, 2016, doi:<a href=\"https://doi.org/10.1371/journal.pone.0164037\">10.1371/journal.pone.0164037</a>.","short":"H. Matsuno, M. Kudoh, A. Watakabe, T. Yamamori, R. Shigemoto, S. Nagao, PLoS One 11 (2016)."},"date_published":"2016-10-06T00:00:00Z","publisher":"Public Library of Science","file_date_updated":"2020-07-14T12:44:42Z","title":"Distribution and structure of synapses on medial vestibular nuclear neurons targeted by cerebellar flocculus purkinje cells and vestibular nerve in mice: Light and electron microscopy studies","abstract":[{"text":"Adaptations of vestibulo-ocular and optokinetic response eye movements have been studied as an experimental model of cerebellum-dependent motor learning. Several previous physiological and pharmacological studies have consistently suggested that the cerebellar flocculus (FL) Purkinje cells (P-cells) and the medial vestibular nucleus (MVN) neurons targeted by FL (FL-targeted MVN neurons) may respectively maintain the memory traces of short- and long-term adaptation. To study the basic structures of the FL-MVN synapses by light microscopy (LM) and electron microscopy (EM), we injected green florescence protein (GFP)-expressing lentivirus into FL to anterogradely label the FL P-cell axons in C57BL/6J mice. The FL P-cell axonal boutons were distributed in the magnocellular MVN and in the border region of parvocellular MVN and prepositus hypoglossi (PrH). In the magnocellular MVN, the FL-P cell axons mainly terminated on somata and proximal dendrites. On the other hand, in the parvocellular MVN/PrH, the FL P-cell axonal synaptic boutons mainly terminated on the relatively small-diameter (&lt; 1 μm) distal dendrites of MVN neurons, forming symmetrical synapses. The majority of such parvocellular MVN/PrH neurons were determined to be glutamatergic by immunocytochemistry and in-situ hybridization of GFP expressing transgenic mice. To further examine the spatial relationship between the synapses of FL P-cells and those of vestibular nerve on the neurons of the parvocellular MVN/ PrH, we added injections of biotinylated dextran amine into the semicircular canal and anterogradely labeled vestibular nerve axons in some mice. The MVN dendrites receiving the FL P-cell axonal synaptic boutons often closely apposed vestibular nerve synaptic boutons in both LM and EM studies. Such a partial overlap of synaptic boutons of FL P-cell axons with those of vestibular nerve axons in the distal dendrites of MVN neurons suggests that inhibitory synapses of FL P-cells may influence the function of neighboring excitatory synapses of vestibular nerve in the parvocellular MVN/PrH neurons.","lang":"eng"}],"publication_status":"published","ddc":["570","571"],"acknowledgement":"This work was supported by RIKEN [to SN]; Grant-in-Aid from the Japan Society for the Promotion of Science, https://www.jsps.go.jp/english/e-grants/ [22300112 to SN].","day":"06","year":"2016","doi":"10.1371/journal.pone.0164037","oa":1,"intvolume":"        11","publist_id":"6038","publication":"PLoS One","scopus_import":1},{"status":"public","date_created":"2018-12-11T11:51:06Z","type":"journal_article","_id":"1279","ec_funded":1,"pubrep_id":"690","author":[{"last_name":"Kovács","first_name":"Krisztián","id":"2AB5821E-F248-11E8-B48F-1D18A9856A87","full_name":"Kovács, Krisztián"},{"first_name":"Joseph","last_name":"O'Neill","id":"426376DC-F248-11E8-B48F-1D18A9856A87","full_name":"O'Neill, Joseph"},{"first_name":"Philipp","last_name":"Schönenberger","full_name":"Schönenberger, Philipp","id":"3B9D816C-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Penttonen, Markku","first_name":"Markku","last_name":"Penttonen"},{"full_name":"Rangel Guerrero, Dámaris K","id":"4871BCE6-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8602-4374","last_name":"Rangel Guerrero","first_name":"Dámaris K"},{"orcid":"0000-0002-5193-4036","last_name":"Csicsvari","first_name":"Jozsef L","full_name":"Csicsvari, Jozsef L","id":"3FA14672-F248-11E8-B48F-1D18A9856A87"}],"quality_controlled":"1","project":[{"grant_number":"291734","_id":"25681D80-B435-11E9-9278-68D0E5697425","name":"International IST Postdoc Fellowship Programme","call_identifier":"FP7"},{"call_identifier":"FP7","name":"Memory-related information processing in neuronal circuits of the hippocampus and entorhinal cortex","_id":"257A4776-B435-11E9-9278-68D0E5697425","grant_number":"281511"}],"oa_version":"Published Version","language":[{"iso":"eng"}],"volume":11,"has_accepted_license":"1","month":"10","article_number":"e0164675","file":[{"date_updated":"2020-07-14T12:44:42Z","date_created":"2018-12-12T10:13:26Z","content_type":"application/pdf","file_name":"IST-2016-690-v1+1_journal.pone.0164675.PDF","relation":"main_file","file_size":4353592,"access_level":"open_access","creator":"system","file_id":"5009","checksum":"395895ecb2216e9c39135abaa56b28b3"}],"issue":"10","acknowledgement":"The research leading to these results has received funding from the People Programme (Marie Curie Actions) of the European Union's Seventh Framework Programme (FP7/2007-2013) under REA grant agreement n° [291734] via the IST FELLOWSHIP awarded to Dr. Krisztián A. Kovács and the European Research Council starting grant (acronym: HIPECMEM Project reference: 281511) awarded to Dr. Jozsef Csicsvari. We thank Lauri Viljanto for technical help in building the ripple detector.","year":"2016","doi":"10.1371/journal.pone.0164675","day":"19","oa":1,"publist_id":"6037","intvolume":"        11","publication":"PLoS One","scopus_import":1,"user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"department":[{"_id":"JoCs"}],"date_updated":"2021-01-12T06:49:35Z","citation":{"mla":"Kovács, Krisztián, et al. “Optogenetically Blocking Sharp Wave Ripple Events in Sleep Does Not Interfere with the Formation of Stable Spatial Representation in the CA1 Area of the Hippocampus.” <i>PLoS One</i>, vol. 11, no. 10, e0164675, Public Library of Science, 2016, doi:<a href=\"https://doi.org/10.1371/journal.pone.0164675\">10.1371/journal.pone.0164675</a>.","short":"K. Kovács, J. O’Neill, P. Schönenberger, M. Penttonen, D.K. Rangel Guerrero, J.L. Csicsvari, PLoS One 11 (2016).","apa":"Kovács, K., O’Neill, J., Schönenberger, P., Penttonen, M., Rangel Guerrero, D. K., &#38; Csicsvari, J. L. (2016). Optogenetically blocking sharp wave ripple events in sleep does not interfere with the formation of stable spatial representation in the CA1 area of the hippocampus. <i>PLoS One</i>. Public Library of Science. <a href=\"https://doi.org/10.1371/journal.pone.0164675\">https://doi.org/10.1371/journal.pone.0164675</a>","ama":"Kovács K, O’Neill J, Schönenberger P, Penttonen M, Rangel Guerrero DK, Csicsvari JL. Optogenetically blocking sharp wave ripple events in sleep does not interfere with the formation of stable spatial representation in the CA1 area of the hippocampus. <i>PLoS One</i>. 2016;11(10). doi:<a href=\"https://doi.org/10.1371/journal.pone.0164675\">10.1371/journal.pone.0164675</a>","ista":"Kovács K, O’Neill J, Schönenberger P, Penttonen M, Rangel Guerrero DK, Csicsvari JL. 2016. Optogenetically blocking sharp wave ripple events in sleep does not interfere with the formation of stable spatial representation in the CA1 area of the hippocampus. PLoS One. 11(10), e0164675.","chicago":"Kovács, Krisztián, Joseph O’Neill, Philipp Schönenberger, Markku Penttonen, Dámaris K Rangel Guerrero, and Jozsef L Csicsvari. “Optogenetically Blocking Sharp Wave Ripple Events in Sleep Does Not Interfere with the Formation of Stable Spatial Representation in the CA1 Area of the Hippocampus.” <i>PLoS One</i>. Public Library of Science, 2016. <a href=\"https://doi.org/10.1371/journal.pone.0164675\">https://doi.org/10.1371/journal.pone.0164675</a>.","ieee":"K. Kovács, J. O’Neill, P. Schönenberger, M. Penttonen, D. K. Rangel Guerrero, and J. L. Csicsvari, “Optogenetically blocking sharp wave ripple events in sleep does not interfere with the formation of stable spatial representation in the CA1 area of the hippocampus,” <i>PLoS One</i>, vol. 11, no. 10. Public Library of Science, 2016."},"date_published":"2016-10-19T00:00:00Z","file_date_updated":"2020-07-14T12:44:42Z","publisher":"Public Library of Science","title":"Optogenetically blocking sharp wave ripple events in sleep does not interfere with the formation of stable spatial representation in the CA1 area of the hippocampus","publication_status":"published","abstract":[{"lang":"eng","text":"During hippocampal sharp wave/ripple (SWR) events, previously occurring, sensory inputdriven neuronal firing patterns are replayed. Such replay is thought to be important for plasticity- related processes and consolidation of memory traces. It has previously been shown that the electrical stimulation-induced disruption of SWR events interferes with learning in rodents in different experimental paradigms. On the other hand, the cognitive map theory posits that the plastic changes of the firing of hippocampal place cells constitute the electrophysiological counterpart of the spatial learning, observable at the behavioral level. Therefore, we tested whether intact SWR events occurring during the sleep/rest session after the first exploration of a novel environment are needed for the stabilization of the CA1 code, which process requires plasticity. We found that the newly-formed representation in the CA1 has the same level of stability with optogenetic SWR blockade as with a control manipulation that delivered the same amount of light into the brain. Therefore our results suggest that at least in the case of passive exploratory behavior, SWR-related plasticity is dispensable for the stability of CA1 ensembles."}],"ddc":["570","571"]},{"author":[{"first_name":"Paul","last_name":"Bourgade","full_name":"Bourgade, Paul"},{"last_name":"Erdös","first_name":"László","orcid":"0000-0001-5366-9603","id":"4DBD5372-F248-11E8-B48F-1D18A9856A87","full_name":"Erdös, László"},{"full_name":"Yau, Horngtzer","first_name":"Horngtzer","last_name":"Yau"},{"full_name":"Yin, Jun","first_name":"Jun","last_name":"Yin"}],"project":[{"name":"Random matrices, universality and disordered quantum systems","grant_number":"338804","_id":"258DCDE6-B435-11E9-9278-68D0E5697425","call_identifier":"FP7"}],"oa_version":"Preprint","language":[{"iso":"eng"}],"volume":69,"month":"10","issue":"10","main_file_link":[{"open_access":"1","url":"https://arxiv.org/abs/1407.5606"}],"status":"public","date_created":"2018-12-11T11:51:07Z","_id":"1280","type":"journal_article","ec_funded":1,"user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","date_updated":"2021-01-12T06:49:35Z","department":[{"_id":"LaEr"}],"citation":{"ieee":"P. Bourgade, L. Erdös, H. Yau, and J. Yin, “Fixed energy universality for generalized wigner matrices,” <i>Communications on Pure and Applied Mathematics</i>, vol. 69, no. 10. Wiley-Blackwell, pp. 1815–1881, 2016.","apa":"Bourgade, P., Erdös, L., Yau, H., &#38; Yin, J. (2016). Fixed energy universality for generalized wigner matrices. <i>Communications on Pure and Applied Mathematics</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1002/cpa.21624\">https://doi.org/10.1002/cpa.21624</a>","ama":"Bourgade P, Erdös L, Yau H, Yin J. Fixed energy universality for generalized wigner matrices. <i>Communications on Pure and Applied Mathematics</i>. 2016;69(10):1815-1881. doi:<a href=\"https://doi.org/10.1002/cpa.21624\">10.1002/cpa.21624</a>","chicago":"Bourgade, Paul, László Erdös, Horngtzer Yau, and Jun Yin. “Fixed Energy Universality for Generalized Wigner Matrices.” <i>Communications on Pure and Applied Mathematics</i>. Wiley-Blackwell, 2016. <a href=\"https://doi.org/10.1002/cpa.21624\">https://doi.org/10.1002/cpa.21624</a>.","ista":"Bourgade P, Erdös L, Yau H, Yin J. 2016. Fixed energy universality for generalized wigner matrices. Communications on Pure and Applied Mathematics. 69(10), 1815–1881.","mla":"Bourgade, Paul, et al. “Fixed Energy Universality for Generalized Wigner Matrices.” <i>Communications on Pure and Applied Mathematics</i>, vol. 69, no. 10, Wiley-Blackwell, 2016, pp. 1815–81, doi:<a href=\"https://doi.org/10.1002/cpa.21624\">10.1002/cpa.21624</a>.","short":"P. Bourgade, L. Erdös, H. Yau, J. Yin, Communications on Pure and Applied Mathematics 69 (2016) 1815–1881."},"date_published":"2016-10-01T00:00:00Z","publisher":"Wiley-Blackwell","title":"Fixed energy universality for generalized wigner matrices","abstract":[{"text":"We prove the Wigner-Dyson-Mehta conjecture at fixed energy in the bulk of the spectrum for generalized symmetric and Hermitian Wigner matrices. Previous results concerning the universality of random matrices either require an averaging in the energy parameter or they hold only for Hermitian matrices if the energy parameter is fixed. We develop a homogenization theory of the Dyson Brownian motion and show that microscopic universality follows from mesoscopic statistics.","lang":"eng"}],"publication_status":"published","acknowledgement":"The work of P.B. was partially supported by National Sci-\r\nence Foundation Grant DMS-1208859.  The work of L.E. was partially supported\r\nby ERC Advanced Grant RANMAT 338804.  The work of H.-T. Y. was partially\r\nsupported by National Science Foundation Grant DMS-1307444 and a Simons In-\r\nvestigator award.  The work of J.Y. was partially supported by National Science\r\nFoundation Grant DMS-1207961.  The major part of this research was conducted\r\nwhen all authors were visiting IAS and were also supported by National Science\r\nFoundation Grant DMS-1128255.","doi":"10.1002/cpa.21624","year":"2016","page":"1815 - 1881","day":"01","oa":1,"intvolume":"        69","publist_id":"6036","publication":"Communications on Pure and Applied Mathematics","scopus_import":1},{"oa":1,"acknowledgement":"This work was supported by the Agropolis Foundation (RHIZOPOLIS project to A.G. and P.N., and RTRA 2009-2011 project to F.P.-W.), the Knowledge Biobase Economy European project (KBBE-005-002 Root enhancement for crop improvement to M.P. and P.N.), and the European EURoot project (FP7-KBBE-2011-5 to J.R., A.G., and P.N.). We thank Carine Alcon for the help with analysis of confocal images, Xavier\r\nDumont for assistance with Arabidopsis transformations, staff members of the\r\nInstitut de Biologie Intégrative des Plantes for technical assistance with biological\r\nmaterial culture, and students and trainees for assistance with laboratory work.\r\nConfocal observations were made at the Montpellier RIO Imaging facility.","day":"01","doi":"10.1104/pp.16.01047","page":"1237 - 1248","year":"2016","scopus_import":1,"publist_id":"6035","intvolume":"       172","publication":"Plant Physiology","department":[{"_id":"EvBe"}],"date_updated":"2021-01-12T06:49:36Z","citation":{"ieee":"E. Bouguyon <i>et al.</i>, “Nitrate controls root development through posttranscriptional regulation of the NRT1.1/NPF6.3 transporter sensor,” <i>Plant Physiology</i>, vol. 172, no. 2. American Society of Plant Biologists, pp. 1237–1248, 2016.","ista":"Bouguyon E, Perrine Walker F, Pervent M, Rochette J, Cuesta C, Benková E, Martinière A, Bach L, Krouk G, Gojon A, Nacry P. 2016. Nitrate controls root development through posttranscriptional regulation of the NRT1.1/NPF6.3 transporter sensor. Plant Physiology. 172(2), 1237–1248.","chicago":"Bouguyon, Eléonore, Francine Perrine Walker, Marjorie Pervent, Juliette Rochette, Candela Cuesta, Eva Benková, Alexandre Martinière, et al. “Nitrate Controls Root Development through Posttranscriptional Regulation of the NRT1.1/NPF6.3 Transporter Sensor.” <i>Plant Physiology</i>. American Society of Plant Biologists, 2016. <a href=\"https://doi.org/10.1104/pp.16.01047\">https://doi.org/10.1104/pp.16.01047</a>.","ama":"Bouguyon E, Perrine Walker F, Pervent M, et al. Nitrate controls root development through posttranscriptional regulation of the NRT1.1/NPF6.3 transporter sensor. <i>Plant Physiology</i>. 2016;172(2):1237-1248. doi:<a href=\"https://doi.org/10.1104/pp.16.01047\">10.1104/pp.16.01047</a>","apa":"Bouguyon, E., Perrine Walker, F., Pervent, M., Rochette, J., Cuesta, C., Benková, E., … Nacry, P. (2016). Nitrate controls root development through posttranscriptional regulation of the NRT1.1/NPF6.3 transporter sensor. <i>Plant Physiology</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1104/pp.16.01047\">https://doi.org/10.1104/pp.16.01047</a>","mla":"Bouguyon, Eléonore, et al. “Nitrate Controls Root Development through Posttranscriptional Regulation of the NRT1.1/NPF6.3 Transporter Sensor.” <i>Plant Physiology</i>, vol. 172, no. 2, American Society of Plant Biologists, 2016, pp. 1237–48, doi:<a href=\"https://doi.org/10.1104/pp.16.01047\">10.1104/pp.16.01047</a>.","short":"E. Bouguyon, F. Perrine Walker, M. Pervent, J. Rochette, C. Cuesta, E. Benková, A. Martinière, L. Bach, G. Krouk, A. Gojon, P. Nacry, Plant Physiology 172 (2016) 1237–1248."},"user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","publication_status":"published","abstract":[{"lang":"eng","text":"Plants are able to modulate root growth and development to optimize their nitrogen nutrition. In Arabidopsis (Arabidopsis thaliana), the adaptive root response to nitrate (NO3 -) depends on the NRT1.1/NPF6.3 transporter/sensor. NRT1.1 represses emergence of lateral root primordia (LRPs) at low concentration or absence of NO3 - through its auxin transport activity that lowers auxin accumulation in LR. However, these functional data strongly contrast with the known transcriptional regulation of NRT1.1, which is markedly repressed in LRPs in the absence of NO3 -. To explain this discrepancy, we investigated in detail the spatiotemporal expression pattern of the NRT1.1 protein during LRP development and combined local transcript analysis with the use of transgenic lines expressing tagged NRT1.1 proteins. Our results show that although NO3 - stimulates NRT1.1 transcription and probably mRNA stability both in primary root tissues and in LRPs, it acts differentially on protein accumulation, depending on the tissues considered with stimulation in cortex and epidermis of the primary root and a strong repression in LRPs and to a lower extent at the primary root tip. This demonstrates that NRT1.1 is strongly regulated at the posttranscriptional level by tissue-specific mechanisms. These mechanisms are crucial for controlling the large palette of adaptive responses to NO3 - mediated by NRT1.1 as they ensure that the protein is present in the proper tissue under the specific conditions where it plays a signaling role in this particular tissue."}],"date_published":"2016-10-01T00:00:00Z","publisher":"American Society of Plant Biologists","title":"Nitrate controls root development through posttranscriptional regulation of the NRT1.1/NPF6.3 transporter sensor","date_created":"2018-12-11T11:51:07Z","type":"journal_article","_id":"1281","status":"public","main_file_link":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5047109/","open_access":"1"}],"quality_controlled":"1","oa_version":"Preprint","author":[{"full_name":"Bouguyon, Eléonore","first_name":"Eléonore","last_name":"Bouguyon"},{"full_name":"Perrine Walker, Francine","last_name":"Perrine Walker","first_name":"Francine"},{"last_name":"Pervent","first_name":"Marjorie","full_name":"Pervent, Marjorie"},{"last_name":"Rochette","first_name":"Juliette","full_name":"Rochette, Juliette"},{"full_name":"Cuesta, Candela","id":"33A3C818-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-1923-2410","first_name":"Candela","last_name":"Cuesta"},{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","full_name":"Benková, Eva","last_name":"Benková","first_name":"Eva","orcid":"0000-0002-8510-9739"},{"first_name":"Alexandre","last_name":"Martinière","full_name":"Martinière, Alexandre"},{"first_name":"Lien","last_name":"Bach","full_name":"Bach, Lien"},{"first_name":"Gabriel","last_name":"Krouk","full_name":"Krouk, Gabriel"},{"full_name":"Gojon, Alain","last_name":"Gojon","first_name":"Alain"},{"first_name":"Philippe","last_name":"Nacry","full_name":"Nacry, Philippe"}],"month":"10","issue":"2","language":[{"iso":"eng"}],"volume":172},{"date_created":"2018-12-11T11:51:07Z","oa":1,"type":"journal_article","_id":"1282","day":"01","status":"public","year":"2016","doi":"10.1007/s11856-016-1419-1","page":"545 - 582","main_file_link":[{"url":"https://arxiv.org/abs/1411.4906","open_access":"1"}],"scopus_import":1,"publist_id":"6034","intvolume":"       216","publication":"Israel Journal of Mathematics","department":[{"_id":"UlWa"}],"quality_controlled":"1","date_updated":"2021-01-12T06:49:36Z","citation":{"ieee":"A. Gundert and U. Wagner, “On eigenvalues of random complexes,” <i>Israel Journal of Mathematics</i>, vol. 216, no. 2. Springer, pp. 545–582, 2016.","apa":"Gundert, A., &#38; Wagner, U. (2016). On eigenvalues of random complexes. <i>Israel Journal of Mathematics</i>. Springer. <a href=\"https://doi.org/10.1007/s11856-016-1419-1\">https://doi.org/10.1007/s11856-016-1419-1</a>","chicago":"Gundert, Anna, and Uli Wagner. “On Eigenvalues of Random Complexes.” <i>Israel Journal of Mathematics</i>. Springer, 2016. <a href=\"https://doi.org/10.1007/s11856-016-1419-1\">https://doi.org/10.1007/s11856-016-1419-1</a>.","ista":"Gundert A, Wagner U. 2016. On eigenvalues of random complexes. Israel Journal of Mathematics. 216(2), 545–582.","ama":"Gundert A, Wagner U. On eigenvalues of random complexes. <i>Israel Journal of Mathematics</i>. 2016;216(2):545-582. doi:<a href=\"https://doi.org/10.1007/s11856-016-1419-1\">10.1007/s11856-016-1419-1</a>","short":"A. Gundert, U. Wagner, Israel Journal of Mathematics 216 (2016) 545–582.","mla":"Gundert, Anna, and Uli Wagner. “On Eigenvalues of Random Complexes.” <i>Israel Journal of Mathematics</i>, vol. 216, no. 2, Springer, 2016, pp. 545–82, doi:<a href=\"https://doi.org/10.1007/s11856-016-1419-1\">10.1007/s11856-016-1419-1</a>."},"oa_version":"Preprint","author":[{"last_name":"Gundert","first_name":"Anna","full_name":"Gundert, Anna"},{"last_name":"Wagner","first_name":"Uli","orcid":"0000-0002-1494-0568","id":"36690CA2-F248-11E8-B48F-1D18A9856A87","full_name":"Wagner, Uli"}],"user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","month":"10","publication_status":"published","abstract":[{"text":"We consider higher-dimensional generalizations of the normalized Laplacian and the adjacency matrix of graphs and study their eigenvalues for the Linial–Meshulam model Xk(n, p) of random k-dimensional simplicial complexes on n vertices. We show that for p = Ω(logn/n), the eigenvalues of each of the matrices are a.a.s. concentrated around two values. The main tool, which goes back to the work of Garland, are arguments that relate the eigenvalues of these matrices to those of graphs that arise as links of (k - 2)-dimensional faces. Garland’s result concerns the Laplacian; we develop an analogous result for the adjacency matrix. The same arguments apply to other models of random complexes which allow for dependencies between the choices of k-dimensional simplices. In the second part of the paper, we apply this to the question of possible higher-dimensional analogues of the discrete Cheeger inequality, which in the classical case of graphs relates the eigenvalues of a graph and its edge expansion. It is very natural to ask whether this generalizes to higher dimensions and, in particular, whether the eigenvalues of the higher-dimensional Laplacian capture the notion of coboundary expansion—a higher-dimensional generalization of edge expansion that arose in recent work of Linial and Meshulam and of Gromov; this question was raised, for instance, by Dotterrer and Kahle. We show that this most straightforward version of a higher-dimensional discrete Cheeger inequality fails, in quite a strong way: For every k ≥ 2 and n ∈ N, there is a k-dimensional complex Yn k on n vertices that has strong spectral expansion properties (all nontrivial eigenvalues of the normalised k-dimensional Laplacian lie in the interval [1−O(1/√1), 1+0(1/√1]) but whose coboundary expansion is bounded from above by O(log n/n) and so tends to zero as n → ∞; moreover, Yn k can be taken to have vanishing integer homology in dimension less than k.","lang":"eng"}],"issue":"2","language":[{"iso":"eng"}],"publisher":"Springer","date_published":"2016-10-01T00:00:00Z","volume":216,"title":"On eigenvalues of random complexes"},{"month":"10","has_accepted_license":"1","issue":"10","file":[{"checksum":"4d569977fad7a7f22b7e3424003d2ab1","file_id":"4679","creator":"system","access_level":"local","file_size":229094,"relation":"main_file","file_name":"IST-2018-1018-v1+1_Zhu_and_Benkova_TIPS_2016.pdf","content_type":"application/pdf","date_created":"2018-12-12T10:08:19Z","date_updated":"2020-07-14T12:44:42Z"}],"language":[{"iso":"eng"}],"volume":21,"quality_controlled":"1","project":[{"grant_number":"I 1774-B16","_id":"2542D156-B435-11E9-9278-68D0E5697425","name":"Hormone cross-talk drives nutrient dependent plant development","call_identifier":"FWF"}],"oa_version":"Submitted Version","author":[{"full_name":"Zhu, Qiang","id":"40A4B9E6-F248-11E8-B48F-1D18A9856A87","first_name":"Qiang","last_name":"Zhu"},{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","full_name":"Benková, Eva","first_name":"Eva","last_name":"Benková","orcid":"0000-0002-8510-9739"}],"pubrep_id":"1018","date_created":"2018-12-11T11:51:08Z","type":"journal_article","_id":"1283","article_type":"original","status":"public","abstract":[{"lang":"eng","text":"The impact of the plant hormone ethylene on seedling development has long been recognized; however, its ecophysiological relevance is unexplored. Three recent studies demonstrate that ethylene is a critical endogenous integrator of various environmental signals including mechanical stress, light, and oxygen availability during seedling germination and growth through the soil."}],"publication_status":"published","ddc":["575"],"publisher":"Cell Press","file_date_updated":"2020-07-14T12:44:42Z","date_published":"2016-10-01T00:00:00Z","title":"Seedlings’ strategy to overcome a soil barrier","date_updated":"2021-01-12T06:49:36Z","department":[{"_id":"EvBe"}],"citation":{"ista":"Zhu Q, Benková E. 2016. Seedlings’ strategy to overcome a soil barrier. Trends in Plant Science. 21(10), 809–811.","ama":"Zhu Q, Benková E. Seedlings’ strategy to overcome a soil barrier. <i>Trends in Plant Science</i>. 2016;21(10):809-811. doi:<a href=\"https://doi.org/10.1016/j.tplants.2016.08.003\">10.1016/j.tplants.2016.08.003</a>","chicago":"Zhu, Qiang, and Eva Benková. “Seedlings’ Strategy to Overcome a Soil Barrier.” <i>Trends in Plant Science</i>. Cell Press, 2016. <a href=\"https://doi.org/10.1016/j.tplants.2016.08.003\">https://doi.org/10.1016/j.tplants.2016.08.003</a>.","apa":"Zhu, Q., &#38; Benková, E. (2016). Seedlings’ strategy to overcome a soil barrier. <i>Trends in Plant Science</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.tplants.2016.08.003\">https://doi.org/10.1016/j.tplants.2016.08.003</a>","ieee":"Q. Zhu and E. Benková, “Seedlings’ strategy to overcome a soil barrier,” <i>Trends in Plant Science</i>, vol. 21, no. 10. Cell Press, pp. 809–811, 2016.","short":"Q. Zhu, E. Benková, Trends in Plant Science 21 (2016) 809–811.","mla":"Zhu, Qiang, and Eva Benková. “Seedlings’ Strategy to Overcome a Soil Barrier.” <i>Trends in Plant Science</i>, vol. 21, no. 10, Cell Press, 2016, pp. 809–11, doi:<a href=\"https://doi.org/10.1016/j.tplants.2016.08.003\">10.1016/j.tplants.2016.08.003</a>."},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)"},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","scopus_import":1,"intvolume":"        21","publist_id":"6033","publication":"Trends in Plant Science","acknowledgement":"This work was supported by the Austrian Science Fund (FWF01_I1774S) to E.B., the Natural Science Foundation of Fujian Province (2016J01099), and the Fujian–Taiwan Joint Innovative Center for Germplasm Resources and Cultivation of Crops (FJ 2011 Program, No 2015-75) to Q.Z. The\r\nauthors\r\nthank\r\nIsrael\r\nAusin\r\nand\r\nXu\r\nChen\r\nfor\r\ncritical\r\nreading\r\nof\r\nthe\r\nmanuscript.","year":"2016","day":"01","doi":"10.1016/j.tplants.2016.08.003","page":"809 - 811"},{"language":[{"iso":"eng"}],"volume":113,"article_processing_charge":"No","month":"10","issue":"40","author":[{"full_name":"Eremina, Marina","first_name":"Marina","last_name":"Eremina"},{"last_name":"Unterholzner","first_name":"Simon","full_name":"Unterholzner, Simon"},{"last_name":"Rathnayake","first_name":"Ajith","full_name":"Rathnayake, Ajith"},{"first_name":"Marcos","last_name":"Castellanos","full_name":"Castellanos, Marcos"},{"full_name":"Khan-Djamei, Mamoona","id":"391B5BBC-F248-11E8-B48F-1D18A9856A87","last_name":"Khan-Djamei","first_name":"Mamoona"},{"last_name":"Kügler","first_name":"Karl","full_name":"Kügler, Karl"},{"full_name":"May, Sean","first_name":"Sean","last_name":"May"},{"full_name":"Mayer, Klaus","first_name":"Klaus","last_name":"Mayer"},{"first_name":"Wilfried","last_name":"Rozhon","full_name":"Rozhon, Wilfried"},{"full_name":"Poppenberger, Brigitte","first_name":"Brigitte","last_name":"Poppenberger"}],"quality_controlled":"1","oa_version":"Submitted Version","status":"public","main_file_link":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5056081/","open_access":"1"}],"date_created":"2018-12-11T11:51:08Z","type":"journal_article","_id":"1284","pmid":1,"date_published":"2016-10-04T00:00:00Z","publisher":"National Academy of Sciences","title":"Brassinosteroids participate in the control of basal and acquired freezing tolerance of plants","publication_status":"published","abstract":[{"text":"Brassinosteroids (BRs) are growth-promoting plant hormones that play a role in abiotic stress responses, but molecular modes that enable this activity remain largely unknown. Here we show that BRs participate in the regulation of freezing tolerance. BR signaling-defective mutants of Arabidopsis thaliana were hypersensitive to freezing before and after cold acclimation. The constitutive activation of BR signaling, in contrast, enhanced freezing resistance. Evidence is provided that the BR-controlled basic helix-loop-helix transcription factor CESTA (CES) can contribute to the constitutive expression of the C-REPEAT/DEHYDRATION-RESPONSIVE ELEMENT BINDING FACTOR (CBF) transcriptional regulators that control cold responsive (COR) gene expression. In addition, CBF-independent classes of BR-regulated COR genes are identified that are regulated in a BR- and CES-dependent manner during cold acclimation. A model is presented in which BRs govern different cold-responsive transcriptional cascades through the post-translational modification of CES and redundantly acting factors. This contributes to the basal resistance against freezing stress, but also to the further improvement of this resistance through cold acclimation.","lang":"eng"}],"external_id":{"pmid":["27489342"]},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","extern":"1","date_updated":"2022-02-18T13:41:37Z","citation":{"short":"M. Eremina, S. Unterholzner, A. Rathnayake, M. Castellanos, M. Khan-Djamei, K. Kügler, S. May, K. Mayer, W. Rozhon, B. Poppenberger, PNAS 113 (2016) E5982–E5991.","mla":"Eremina, Marina, et al. “Brassinosteroids Participate in the Control of Basal and Acquired Freezing Tolerance of Plants.” <i>PNAS</i>, vol. 113, no. 40, National Academy of Sciences, 2016, pp. E5982–91, doi:<a href=\"https://doi.org/10.1073/pnas.1611477113\">10.1073/pnas.1611477113</a>.","ista":"Eremina M, Unterholzner S, Rathnayake A, Castellanos M, Khan-Djamei M, Kügler K, May S, Mayer K, Rozhon W, Poppenberger B. 2016. Brassinosteroids participate in the control of basal and acquired freezing tolerance of plants. PNAS. 113(40), E5982–E5991.","ama":"Eremina M, Unterholzner S, Rathnayake A, et al. Brassinosteroids participate in the control of basal and acquired freezing tolerance of plants. <i>PNAS</i>. 2016;113(40):E5982-E5991. doi:<a href=\"https://doi.org/10.1073/pnas.1611477113\">10.1073/pnas.1611477113</a>","chicago":"Eremina, Marina, Simon Unterholzner, Ajith Rathnayake, Marcos Castellanos, Mamoona Khan-Djamei, Karl Kügler, Sean May, Klaus Mayer, Wilfried Rozhon, and Brigitte Poppenberger. “Brassinosteroids Participate in the Control of Basal and Acquired Freezing Tolerance of Plants.” <i>PNAS</i>. National Academy of Sciences, 2016. <a href=\"https://doi.org/10.1073/pnas.1611477113\">https://doi.org/10.1073/pnas.1611477113</a>.","apa":"Eremina, M., Unterholzner, S., Rathnayake, A., Castellanos, M., Khan-Djamei, M., Kügler, K., … Poppenberger, B. (2016). Brassinosteroids participate in the control of basal and acquired freezing tolerance of plants. <i>PNAS</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.1611477113\">https://doi.org/10.1073/pnas.1611477113</a>","ieee":"M. Eremina <i>et al.</i>, “Brassinosteroids participate in the control of basal and acquired freezing tolerance of plants,” <i>PNAS</i>, vol. 113, no. 40. National Academy of Sciences, pp. E5982–E5991, 2016."},"publist_id":"6032","intvolume":"       113","publication":"PNAS","scopus_import":"1","acknowledgement":"We thank Joanne Chory for seeds of the bee1 bee2 bee3, bes1-D, and bzr1-1D mutants and the 35S:BRI1-GFP line; Irene Ziegler, Clarissa Fahrig, and Renata Milcevicova for technical assistance; and the horticultural staff of the TUMs Gewächshauslaborzentrum Dürnast for plant care. This work was supported by funds from the Austrian Science Fund (Project P22734 to B.P.), the Deutsche Forschungsgemeinschaft (Project PO1640/4 to B.P. and SFB924 to B.P. and K.F.X.M.), and a TUM doctoral fellowship (to M.E.). M.E. and S.J.U. were members of the TUM graduate school. ","day":"04","doi":"10.1073/pnas.1611477113","page":"E5982 - E5991","year":"2016","oa":1},{"publication":"Annual Review of Cell and Developmental Biology","publist_id":"6031","intvolume":"        32","ec_funded":1,"scopus_import":1,"day":"06","status":"public","year":"2016","page":"469 - 490","doi":"10.1146/annurev-cellbio-111315-125341","acknowledgement":"We would like to thank Dani Bodor for critical comments on the manuscript and Guillaume Salbreux for discussions. The authors are supported by the United Kingdom's Medical Research Council (MRC) (E.K.P. and I.M.A.; core funding to the MRC Laboratory for Molecular Cell Biology), by the European Research Council [ERC GA 311637 (E.K.P.) and ERC GA 281556 (M.S.)], and by a START award from the Austrian Science Foundation (M.S.).","type":"journal_article","_id":"1285","date_created":"2018-12-11T11:51:08Z","title":"Focal adhesion-independent cell migration","volume":32,"date_published":"2016-10-06T00:00:00Z","language":[{"iso":"eng"}],"publisher":"Annual Reviews","publication_status":"published","month":"10","abstract":[{"lang":"eng","text":"Cell migration is central to a multitude of physiological processes, including embryonic development, immune surveillance, and wound healing, and deregulated migration is key to cancer dissemination. Decades of investigations have uncovered many of the molecular and physical mechanisms underlying cell migration. Together with protrusion extension and cell body retraction, adhesion to the substrate via specific focal adhesion points has long been considered an essential step in cell migration. Although this is true for cells moving on two-dimensional substrates, recent studies have demonstrated that focal adhesions are not required for cells moving in three dimensions, in which confinement is sufficient to maintain a cell in contact with its substrate. Here, we review the investigations that have led to challenging the requirement of specific adhesions for migration, discuss the physical mechanisms proposed for cell body translocation during focal adhesion-independent migration, and highlight the remaining open questions for the future."}],"user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","author":[{"full_name":"Paluch, Ewa","last_name":"Paluch","first_name":"Ewa"},{"first_name":"Irene","last_name":"Aspalter","full_name":"Aspalter, Irene"},{"first_name":"Michael K","last_name":"Sixt","orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","full_name":"Sixt, Michael K"}],"oa_version":"None","citation":{"mla":"Paluch, Ewa, et al. “Focal Adhesion-Independent Cell Migration.” <i>Annual Review of Cell and Developmental Biology</i>, vol. 32, Annual Reviews, 2016, pp. 469–90, doi:<a href=\"https://doi.org/10.1146/annurev-cellbio-111315-125341\">10.1146/annurev-cellbio-111315-125341</a>.","short":"E. Paluch, I. Aspalter, M.K. Sixt, Annual Review of Cell and Developmental Biology 32 (2016) 469–490.","ieee":"E. Paluch, I. Aspalter, and M. K. Sixt, “Focal adhesion-independent cell migration,” <i>Annual Review of Cell and Developmental Biology</i>, vol. 32. Annual Reviews, pp. 469–490, 2016.","apa":"Paluch, E., Aspalter, I., &#38; Sixt, M. K. (2016). Focal adhesion-independent cell migration. <i>Annual Review of Cell and Developmental Biology</i>. Annual Reviews. <a href=\"https://doi.org/10.1146/annurev-cellbio-111315-125341\">https://doi.org/10.1146/annurev-cellbio-111315-125341</a>","ama":"Paluch E, Aspalter I, Sixt MK. Focal adhesion-independent cell migration. <i>Annual Review of Cell and Developmental Biology</i>. 2016;32:469-490. doi:<a href=\"https://doi.org/10.1146/annurev-cellbio-111315-125341\">10.1146/annurev-cellbio-111315-125341</a>","ista":"Paluch E, Aspalter I, Sixt MK. 2016. Focal adhesion-independent cell migration. Annual Review of Cell and Developmental Biology. 32, 469–490.","chicago":"Paluch, Ewa, Irene Aspalter, and Michael K Sixt. “Focal Adhesion-Independent Cell Migration.” <i>Annual Review of Cell and Developmental Biology</i>. Annual Reviews, 2016. <a href=\"https://doi.org/10.1146/annurev-cellbio-111315-125341\">https://doi.org/10.1146/annurev-cellbio-111315-125341</a>."},"quality_controlled":"1","department":[{"_id":"MiSi"}],"project":[{"call_identifier":"FP7","name":"Cytoskeletal force generation and force transduction of migrating leukocytes (EU)","grant_number":"281556","_id":"25A603A2-B435-11E9-9278-68D0E5697425"},{"call_identifier":"FWF","grant_number":"Y 564-B12","_id":"25A8E5EA-B435-11E9-9278-68D0E5697425","name":"Cytoskeletal force generation and transduction of leukocytes (FWF)"}],"date_updated":"2021-01-12T06:49:37Z"},{"status":"public","main_file_link":[{"url":"https://arxiv.org/abs/1607.06092","open_access":"1"}],"type":"journal_article","_id":"1286","date_created":"2018-12-11T11:51:09Z","ec_funded":1,"author":[{"last_name":"Midya","first_name":"Bikashkali","id":"456187FC-F248-11E8-B48F-1D18A9856A87","full_name":"Midya, Bikashkali"},{"full_name":"Tomza, Michał","first_name":"Michał","last_name":"Tomza"},{"full_name":"Schmidt, Richard","last_name":"Schmidt","first_name":"Richard"},{"full_name":"Lemeshko, Mikhail","id":"37CB05FA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6990-7802","first_name":"Mikhail","last_name":"Lemeshko"}],"oa_version":"Preprint","quality_controlled":"1","project":[{"call_identifier":"FP7","_id":"25681D80-B435-11E9-9278-68D0E5697425","grant_number":"291734","name":"International IST Postdoc Fellowship Programme"}],"volume":94,"language":[{"iso":"eng"}],"article_number":"041601","issue":"4","month":"10","year":"2016","doi":"10.1103/PhysRevA.94.041601","day":"13","acknowledgement":"The work was supported by the NSF through a grant for the Institute for Theoretical Atomic, Molecular, and Optical Physics at Harvard University and the Smithsonian Astrophysical Observatory. B.M. acknowledges financial support received from the People Programme (Marie Curie Actions) of the European Union's Seventh Framework Programme (FP7/2007-2013) under REA grant agreement No. 291734. M.T. acknowledges support from the EU Marie Curie COFUND action (ICFOnest), the EU Grants ERC AdG OSYRIS, FP7 SIQS and EQuaM, FETPROACT QUIC, the Spanish Ministry Grants FOQUS (FIS2013-46768-P) and Severo Ochoa (SEV-2015-0522), Generalitat de Catalunya (SGR 874), Fundacio Cellex, the National Science Centre (2015/19/D/ST4/02173), and the PL-Grid Infrastructure.","oa":1,"publication":"Physical Review A - Atomic, Molecular, and Optical Physics","publist_id":"6030","intvolume":"        94","scopus_import":1,"user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","citation":{"short":"B. Midya, M. Tomza, R. Schmidt, M. Lemeshko, Physical Review A - Atomic, Molecular, and Optical Physics 94 (2016).","mla":"Midya, Bikashkali, et al. “Rotation of Cold Molecular Ions inside a Bose-Einstein Condensate.” <i>Physical Review A - Atomic, Molecular, and Optical Physics</i>, vol. 94, no. 4, 041601, American Physical Society, 2016, doi:<a href=\"https://doi.org/10.1103/PhysRevA.94.041601\">10.1103/PhysRevA.94.041601</a>.","ieee":"B. Midya, M. Tomza, R. Schmidt, and M. Lemeshko, “Rotation of cold molecular ions inside a Bose-Einstein condensate,” <i>Physical Review A - Atomic, Molecular, and Optical Physics</i>, vol. 94, no. 4. American Physical Society, 2016.","chicago":"Midya, Bikashkali, Michał Tomza, Richard Schmidt, and Mikhail Lemeshko. “Rotation of Cold Molecular Ions inside a Bose-Einstein Condensate.” <i>Physical Review A - Atomic, Molecular, and Optical Physics</i>. American Physical Society, 2016. <a href=\"https://doi.org/10.1103/PhysRevA.94.041601\">https://doi.org/10.1103/PhysRevA.94.041601</a>.","ista":"Midya B, Tomza M, Schmidt R, Lemeshko M. 2016. Rotation of cold molecular ions inside a Bose-Einstein condensate. Physical Review A - Atomic, Molecular, and Optical Physics. 94(4), 041601.","ama":"Midya B, Tomza M, Schmidt R, Lemeshko M. Rotation of cold molecular ions inside a Bose-Einstein condensate. <i>Physical Review A - Atomic, Molecular, and Optical Physics</i>. 2016;94(4). doi:<a href=\"https://doi.org/10.1103/PhysRevA.94.041601\">10.1103/PhysRevA.94.041601</a>","apa":"Midya, B., Tomza, M., Schmidt, R., &#38; Lemeshko, M. (2016). Rotation of cold molecular ions inside a Bose-Einstein condensate. <i>Physical Review A - Atomic, Molecular, and Optical Physics</i>. American Physical Society. <a href=\"https://doi.org/10.1103/PhysRevA.94.041601\">https://doi.org/10.1103/PhysRevA.94.041601</a>"},"department":[{"_id":"MiLe"}],"date_updated":"2021-01-12T06:49:37Z","title":"Rotation of cold molecular ions inside a Bose-Einstein condensate","date_published":"2016-10-13T00:00:00Z","publisher":"American Physical Society","publication_status":"published","abstract":[{"text":"We use recently developed angulon theory [R. Schmidt and M. Lemeshko, Phys. Rev. Lett. 114, 203001 (2015)PRLTAO0031-900710.1103/PhysRevLett.114.203001] to study the rotational spectrum of a cyanide molecular anion immersed into Bose-Einstein condensates of rubidium and strontium. Based on ab initio potential energy surfaces, we provide a detailed study of the rotational Lamb shift and many-body-induced fine structure which arise due to dressing of molecular rotation by a field of phonon excitations. We demonstrate that the magnitude of these effects is large enough in order to be observed in modern experiments on cold molecular ions. Furthermore, we introduce a novel method to construct pseudopotentials starting from the ab initio potential energy surfaces, which provides a means to obtain effective coupling constants for low-energy polaron models.","lang":"eng"}]},{"publication_status":"published","abstract":[{"text":"A planar waveguide with an impedance boundary, composed of nonperfect metallic plates, and with passive or active dielectric filling, is considered. We show the possibility of selective mode guiding and amplification when a homogeneous pump is added to the dielectric and analyze differences in TE and TM mode propagation. Such a non-conservative system is also shown to feature exceptional points for specific and experimentally tunable parameters, which are described for a particular case of transparent dielectric.","lang":"eng"}],"publisher":"Optica Publishing Group","date_published":"2016-10-15T00:00:00Z","title":"Modes and exceptional points in waveguides with impedance boundary conditions","department":[{"_id":"MiLe"}],"date_updated":"2023-10-17T12:16:24Z","citation":{"ista":"Midya B, Konotop V. 2016. Modes and exceptional points in waveguides with impedance boundary conditions. Optics Letters. 41(20), 4621–4624.","ama":"Midya B, Konotop V. Modes and exceptional points in waveguides with impedance boundary conditions. <i>Optics Letters</i>. 2016;41(20):4621-4624. doi:<a href=\"https://doi.org/10.1364/OL.41.004621\">10.1364/OL.41.004621</a>","chicago":"Midya, Bikashkali, and Vladimir Konotop. “Modes and Exceptional Points in Waveguides with Impedance Boundary Conditions.” <i>Optics Letters</i>. Optica Publishing Group, 2016. <a href=\"https://doi.org/10.1364/OL.41.004621\">https://doi.org/10.1364/OL.41.004621</a>.","apa":"Midya, B., &#38; Konotop, V. (2016). Modes and exceptional points in waveguides with impedance boundary conditions. <i>Optics Letters</i>. Optica Publishing Group. <a href=\"https://doi.org/10.1364/OL.41.004621\">https://doi.org/10.1364/OL.41.004621</a>","ieee":"B. Midya and V. Konotop, “Modes and exceptional points in waveguides with impedance boundary conditions,” <i>Optics Letters</i>, vol. 41, no. 20. Optica Publishing Group, pp. 4621–4624, 2016.","mla":"Midya, Bikashkali, and Vladimir Konotop. “Modes and Exceptional Points in Waveguides with Impedance Boundary Conditions.” <i>Optics Letters</i>, vol. 41, no. 20, Optica Publishing Group, 2016, pp. 4621–24, doi:<a href=\"https://doi.org/10.1364/OL.41.004621\">10.1364/OL.41.004621</a>.","short":"B. Midya, V. Konotop, Optics Letters 41 (2016) 4621–4624."},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","scopus_import":"1","publist_id":"6029","intvolume":"        41","publication":"Optics Letters","oa":1,"acknowledgement":"The research of B.M. is supported by the People Programme (Marie Curie Actions) of the European Union’s Seventh Framework Programme (FP7/2007-2013) under REA grant No. [291734].","page":"4621 - 4624","day":"15","year":"2016","doi":"10.1364/OL.41.004621","month":"10","issue":"20","language":[{"iso":"eng"}],"volume":41,"article_processing_charge":"No","quality_controlled":"1","project":[{"name":"International IST Postdoc Fellowship Programme","_id":"25681D80-B435-11E9-9278-68D0E5697425","grant_number":"291734","call_identifier":"FP7"}],"oa_version":"Preprint","author":[{"last_name":"Midya","first_name":"Bikashkali","id":"456187FC-F248-11E8-B48F-1D18A9856A87","full_name":"Midya, Bikashkali"},{"full_name":"Konotop, Vladimir","last_name":"Konotop","first_name":"Vladimir"}],"ec_funded":1,"date_created":"2018-12-11T11:51:09Z","type":"journal_article","_id":"1287","main_file_link":[{"open_access":"1","url":"https://arxiv.org/abs/1609.02863"}],"status":"public"},{"scopus_import":1,"publication":"Biochimica et Biophysica Acta - Bioenergetics","publist_id":"6028","intvolume":"      1857","type":"journal_article","_id":"1288","date_created":"2018-12-11T11:51:09Z","doi":"10.1016/j.bbabio.2016.08.008","year":"2016","page":"1777 - 1785","status":"public","day":"01","acknowledgement":"This work was funded by the UK Medical Research Council.","issue":"11","publication_status":"published","month":"11","abstract":[{"lang":"eng","text":"Respiratory complex I transfers electrons from NADH to quinone, utilizing the reaction energy to translocate protons across the membrane. It is a key enzyme of the respiratory chain of many prokaryotic and most eukaryotic organisms. The reversible NADH oxidation reaction is facilitated in complex I by non-covalently bound flavin mononucleotide (FMN). Here we report that the catalytic activity of E. coli complex I with artificial electron acceptors potassium ferricyanide (FeCy) and hexaamineruthenium (HAR) is significantly inhibited in the enzyme pre-reduced by NADH. Further, we demonstrate that the inhibition is caused by reversible dissociation of FMN. The binding constant (Kd) for FMN increases from the femto- or picomolar range in oxidized complex I to the nanomolar range in the NADH reduced enzyme, with an FMN dissociation time constant of ~ 5 s. The oxidation state of complex I, rather than that of FMN, proved critical to the dissociation. Such dissociation is not observed with the T. thermophilus enzyme and our analysis suggests that the difference may be due to the unusually high redox potential of Fe-S cluster N1a in E. coli. It is possible that the enzyme attenuates ROS production in vivo by releasing FMN under highly reducing conditions."}],"volume":1857,"title":"Reversible FMN dissociation from Escherichia coli respiratory complex I","publisher":"Elsevier","date_published":"2016-11-01T00:00:00Z","language":[{"iso":"eng"}],"citation":{"ieee":"P. Holt, R. Efremov, E. Nakamaru Ogiso, and L. A. Sazanov, “Reversible FMN dissociation from Escherichia coli respiratory complex I,” <i>Biochimica et Biophysica Acta - Bioenergetics</i>, vol. 1857, no. 11. Elsevier, pp. 1777–1785, 2016.","ama":"Holt P, Efremov R, Nakamaru Ogiso E, Sazanov LA. Reversible FMN dissociation from Escherichia coli respiratory complex I. <i>Biochimica et Biophysica Acta - Bioenergetics</i>. 2016;1857(11):1777-1785. doi:<a href=\"https://doi.org/10.1016/j.bbabio.2016.08.008\">10.1016/j.bbabio.2016.08.008</a>","ista":"Holt P, Efremov R, Nakamaru Ogiso E, Sazanov LA. 2016. Reversible FMN dissociation from Escherichia coli respiratory complex I. Biochimica et Biophysica Acta - Bioenergetics. 1857(11), 1777–1785.","chicago":"Holt, Peter, Rouslan Efremov, Eiko Nakamaru Ogiso, and Leonid A Sazanov. “Reversible FMN Dissociation from Escherichia Coli Respiratory Complex I.” <i>Biochimica et Biophysica Acta - Bioenergetics</i>. Elsevier, 2016. <a href=\"https://doi.org/10.1016/j.bbabio.2016.08.008\">https://doi.org/10.1016/j.bbabio.2016.08.008</a>.","apa":"Holt, P., Efremov, R., Nakamaru Ogiso, E., &#38; Sazanov, L. A. (2016). Reversible FMN dissociation from Escherichia coli respiratory complex I. <i>Biochimica et Biophysica Acta - Bioenergetics</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.bbabio.2016.08.008\">https://doi.org/10.1016/j.bbabio.2016.08.008</a>","short":"P. Holt, R. Efremov, E. Nakamaru Ogiso, L.A. Sazanov, Biochimica et Biophysica Acta - Bioenergetics 1857 (2016) 1777–1785.","mla":"Holt, Peter, et al. “Reversible FMN Dissociation from Escherichia Coli Respiratory Complex I.” <i>Biochimica et Biophysica Acta - Bioenergetics</i>, vol. 1857, no. 11, Elsevier, 2016, pp. 1777–85, doi:<a href=\"https://doi.org/10.1016/j.bbabio.2016.08.008\">10.1016/j.bbabio.2016.08.008</a>."},"oa_version":"None","quality_controlled":"1","department":[{"_id":"LeSa"}],"date_updated":"2021-01-12T06:49:38Z","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","author":[{"first_name":"Peter","last_name":"Holt","full_name":"Holt, Peter"},{"last_name":"Efremov","first_name":"Rouslan","full_name":"Efremov, Rouslan"},{"last_name":"Nakamaru Ogiso","first_name":"Eiko","full_name":"Nakamaru Ogiso, Eiko"},{"full_name":"Sazanov, Leonid A","id":"338D39FE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0977-7989","last_name":"Sazanov","first_name":"Leonid A"}]},{"scopus_import":1,"related_material":{"record":[{"status":"public","id":"1568","relation":"earlier_version"}]},"publication":"Pattern Recognition Letters","publist_id":"6027","intvolume":"        83","oa":1,"doi":"10.1016/j.patrec.2015.12.012","day":"01","year":"2016","page":"13 - 22","ddc":["004","514"],"publication_status":"published","abstract":[{"text":"Aiming at the automatic diagnosis of tumors using narrow band imaging (NBI) magnifying endoscopic (ME) images of the stomach, we combine methods from image processing, topology, geometry, and machine learning to classify patterns into three classes: oval, tubular and irregular. Training the algorithm on a small number of images of each type, we achieve a high rate of correct classifications. The analysis of the learning algorithm reveals that a handful of geometric and topological features are responsible for the overwhelming majority of decisions.","lang":"eng"}],"title":"The classification of endoscopy images with persistent homology","date_published":"2016-11-01T00:00:00Z","file_date_updated":"2020-07-14T12:44:42Z","publisher":"Elsevier","citation":{"chicago":"Dunaeva, Olga, Herbert Edelsbrunner, Anton Lukyanov, Michael Machin, Daria Malkova, Roman Kuvaev, and Sergey Kashin. “The Classification of Endoscopy Images with Persistent Homology.” <i>Pattern Recognition Letters</i>. Elsevier, 2016. <a href=\"https://doi.org/10.1016/j.patrec.2015.12.012\">https://doi.org/10.1016/j.patrec.2015.12.012</a>.","ista":"Dunaeva O, Edelsbrunner H, Lukyanov A, Machin M, Malkova D, Kuvaev R, Kashin S. 2016. The classification of endoscopy images with persistent homology. Pattern Recognition Letters. 83(1), 13–22.","ama":"Dunaeva O, Edelsbrunner H, Lukyanov A, et al. The classification of endoscopy images with persistent homology. <i>Pattern Recognition Letters</i>. 2016;83(1):13-22. doi:<a href=\"https://doi.org/10.1016/j.patrec.2015.12.012\">10.1016/j.patrec.2015.12.012</a>","apa":"Dunaeva, O., Edelsbrunner, H., Lukyanov, A., Machin, M., Malkova, D., Kuvaev, R., &#38; Kashin, S. (2016). The classification of endoscopy images with persistent homology. <i>Pattern Recognition Letters</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.patrec.2015.12.012\">https://doi.org/10.1016/j.patrec.2015.12.012</a>","ieee":"O. Dunaeva <i>et al.</i>, “The classification of endoscopy images with persistent homology,” <i>Pattern Recognition Letters</i>, vol. 83, no. 1. Elsevier, pp. 13–22, 2016.","mla":"Dunaeva, Olga, et al. “The Classification of Endoscopy Images with Persistent Homology.” <i>Pattern Recognition Letters</i>, vol. 83, no. 1, Elsevier, 2016, pp. 13–22, doi:<a href=\"https://doi.org/10.1016/j.patrec.2015.12.012\">10.1016/j.patrec.2015.12.012</a>.","short":"O. Dunaeva, H. Edelsbrunner, A. Lukyanov, M. Machin, D. Malkova, R. Kuvaev, S. Kashin, Pattern Recognition Letters 83 (2016) 13–22."},"department":[{"_id":"HeEd"}],"date_updated":"2023-02-23T10:04:40Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)"},"pubrep_id":"975","type":"journal_article","_id":"1289","date_created":"2018-12-11T11:51:10Z","status":"public","file":[{"relation":"main_file","creator":"dernst","checksum":"33458bbb8c32a339e1adeca6d5a1112d","file_id":"6334","file_size":1921113,"access_level":"open_access","content_type":"application/pdf","date_updated":"2020-07-14T12:44:42Z","date_created":"2019-04-17T07:55:51Z","file_name":"2016-Edelsbrunner_The_classification.pdf"}],"issue":"1","has_accepted_license":"1","month":"11","volume":83,"article_processing_charge":"No","language":[{"iso":"eng"}],"oa_version":"Submitted Version","quality_controlled":"1","author":[{"full_name":"Dunaeva, Olga","first_name":"Olga","last_name":"Dunaeva"},{"full_name":"Edelsbrunner, Herbert","id":"3FB178DA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-9823-6833","first_name":"Herbert","last_name":"Edelsbrunner"},{"full_name":"Lukyanov, Anton","last_name":"Lukyanov","first_name":"Anton"},{"full_name":"Machin, Michael","first_name":"Michael","last_name":"Machin"},{"full_name":"Malkova, Daria","last_name":"Malkova","first_name":"Daria"},{"first_name":"Roman","last_name":"Kuvaev","full_name":"Kuvaev, Roman"},{"full_name":"Kashin, Sergey","first_name":"Sergey","last_name":"Kashin"}]},{"scopus_import":1,"intvolume":"        12","publist_id":"6026","publication":"Nature Chemical Biology","oa":1,"acknowledgement":"This work was supported in part by National Institute of Allergy and Infectious Diseases grant U54 AI057159, US National Institutes of Health grants R01 GM081617 (to R.K.) and GM086258 (to J.C.), European Research Council FP7 ERC grant 281891 (to R.K.) and a National Science Foundation Graduate Fellowship (to L.K.S.).\r\n","year":"2016","day":"01","doi":"10.1038/nchembio.2176","page":"902 - 904","abstract":[{"text":"We developed a competition-based screening strategy to identify compounds that invert the selective advantage of antibiotic resistance. Using our assay, we screened over 19,000 compounds for the ability to select against the TetA tetracycline-resistance efflux pump in Escherichia coli and identified two hits, β-thujaplicin and disulfiram. Treating a tetracycline-resistant population with β-thujaplicin selects for loss of the resistance gene, enabling an effective second-phase treatment with doxycycline.","lang":"eng"}],"publication_status":"published","publisher":"Nature Publishing Group","date_published":"2016-11-01T00:00:00Z","title":"Compounds that select against the tetracycline-resistance efflux pump","date_updated":"2021-01-12T06:49:39Z","department":[{"_id":"CaGu"},{"_id":"GaTk"}],"citation":{"ieee":"L. Stone, M. Baym, T. Lieberman, R. P. Chait, J. Clardy, and R. Kishony, “Compounds that select against the tetracycline-resistance efflux pump,” <i>Nature Chemical Biology</i>, vol. 12, no. 11. Nature Publishing Group, pp. 902–904, 2016.","apa":"Stone, L., Baym, M., Lieberman, T., Chait, R. P., Clardy, J., &#38; Kishony, R. (2016). Compounds that select against the tetracycline-resistance efflux pump. <i>Nature Chemical Biology</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/nchembio.2176\">https://doi.org/10.1038/nchembio.2176</a>","ista":"Stone L, Baym M, Lieberman T, Chait RP, Clardy J, Kishony R. 2016. Compounds that select against the tetracycline-resistance efflux pump. Nature Chemical Biology. 12(11), 902–904.","ama":"Stone L, Baym M, Lieberman T, Chait RP, Clardy J, Kishony R. Compounds that select against the tetracycline-resistance efflux pump. <i>Nature Chemical Biology</i>. 2016;12(11):902-904. doi:<a href=\"https://doi.org/10.1038/nchembio.2176\">10.1038/nchembio.2176</a>","chicago":"Stone, Laura, Michael Baym, Tami Lieberman, Remy P Chait, Jon Clardy, and Roy Kishony. “Compounds That Select against the Tetracycline-Resistance Efflux Pump.” <i>Nature Chemical Biology</i>. Nature Publishing Group, 2016. <a href=\"https://doi.org/10.1038/nchembio.2176\">https://doi.org/10.1038/nchembio.2176</a>.","short":"L. Stone, M. Baym, T. Lieberman, R.P. Chait, J. Clardy, R. Kishony, Nature Chemical Biology 12 (2016) 902–904.","mla":"Stone, Laura, et al. “Compounds That Select against the Tetracycline-Resistance Efflux Pump.” <i>Nature Chemical Biology</i>, vol. 12, no. 11, Nature Publishing Group, 2016, pp. 902–04, doi:<a href=\"https://doi.org/10.1038/nchembio.2176\">10.1038/nchembio.2176</a>."},"user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","date_created":"2018-12-11T11:51:10Z","_id":"1290","type":"journal_article","status":"public","main_file_link":[{"open_access":"1","url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5069154/"}],"month":"11","issue":"11","language":[{"iso":"eng"}],"volume":12,"quality_controlled":"1","oa_version":"Preprint","author":[{"first_name":"Laura","last_name":"Stone","full_name":"Stone, Laura"},{"first_name":"Michael","last_name":"Baym","full_name":"Baym, Michael"},{"full_name":"Lieberman, Tami","last_name":"Lieberman","first_name":"Tami"},{"orcid":"0000-0003-0876-3187","first_name":"Remy P","last_name":"Chait","full_name":"Chait, Remy P","id":"3464AE84-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Clardy","first_name":"Jon","full_name":"Clardy, Jon"},{"full_name":"Kishony, Roy","last_name":"Kishony","first_name":"Roy"}]}]