[{"type":"dissertation","language":[{"iso":"eng"}],"doi":"10.15479/AT:ISTA:7680","publisher":"Institute of Science and Technology Austria","year":"2020","date_published":"2020-04-24T00:00:00Z","author":[{"last_name":"Kainrath","id":"32CFBA64-F248-11E8-B48F-1D18A9856A87","first_name":"Stephanie","full_name":"Kainrath, Stephanie"}],"oa":1,"_id":"7680","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","article_processing_charge":"No","ddc":["570"],"file_date_updated":"2021-10-31T23:30:05Z","date_created":"2020-04-24T16:00:51Z","department":[{"_id":"CaGu"}],"supervisor":[{"last_name":"Janovjak","id":"33BA6C30-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8023-9315","full_name":"Janovjak, Harald L","first_name":"Harald L"}],"month":"04","publication_identifier":{"eissn":["2663-337X"]},"status":"public","date_updated":"2023-09-22T09:20:10Z","oa_version":"None","publication_status":"published","page":"98","day":"24","abstract":[{"text":"Proteins and their complex dynamic interactions regulate cellular mechanisms from sensing and transducing extracellular signals, to mediating genetic responses, and sustaining or changing cell morphology. To manipulate these protein-protein interactions (PPIs) that govern the behavior and fate of cells, synthetically constructed, genetically encoded tools provide the means to precisely target proteins of interest (POIs), and control their subcellular localization and activity in vitro and in vivo. Ideal synthetic tools react to an orthogonal cue, i.e. a trigger that does not activate any other endogenous process, thereby allowing manipulation of the POI alone.\r\nIn optogenetics, naturally occurring photosensory domain from plants, algae and bacteria are re-purposed and genetically fused to POIs. Illumination with light of a specific wavelength triggers a conformational change that can mediate PPIs, such as dimerization or oligomerization. By using light as a trigger, these tools can be activated with high spatial and temporal precision, on subcellular and millisecond scales. Chemogenetic tools consist of protein domains that recognize and bind small molecules. By genetic fusion to POIs, these domains can mediate PPIs upon addition of their specific ligands, which are often synthetically designed to provide highly specific interactions and exhibit good bioavailability.\r\nMost optogenetic tools to mediate PPIs are based on well-studied photoreceptors responding to red, blue or near-UV light, leaving a striking gap in the green band of the visible light spectrum. Among both optogenetic and chemogenetic tools, there is an abundance of methods to induce PPIs, but tools to disrupt them require UV illumination, rely on covalent linkage and subsequent enzymatic cleavage or initially result in protein clustering of unknown stoichiometry.\r\nThis work describes how the recently structurally and photochemically characterized green-light responsive cobalamin-binding domains (CBDs) from bacterial transcription factors were re-purposed to function as a green-light responsive optogenetic tool. In contrast to previously engineered optogenetic tools, CBDs do not induce PPI, but rather confer a PPI already upon expression, which can be rapidly disrupted by illumination. This was employed to mimic inhibition of constitutive activity of a growth factor receptor, and successfully implement for cell signalling in mammalian cells and in vivo to rescue development in zebrafish. This work further describes the development and application of a chemically induced de-dimerizer (CDD) based on a recently identified and structurally described bacterial oxyreductase. CDD forms a dimer upon expression in absence of its cofactor, the flavin derivative F420. Safety and of domain expression and ligand exposure are demonstrated in vitro and in vivo in zebrafish. The system is further applied to inhibit cell signalling output from a chimeric receptor upon F420 treatment.\r\nCBDs and CDD expand the repertoire of synthetic tools by providing novel mechanisms of mediating PPIs, and by recognizing previously not utilized cues. In the future, they can readily be combined with existing synthetic tools to functionally manipulate PPIs in vitro and in vivo.","lang":"eng"}],"degree_awarded":"PhD","alternative_title":["ISTA Thesis"],"has_accepted_license":"1","citation":{"chicago":"Kainrath, Stephanie. “Synthetic Tools for Optogenetic and Chemogenetic Inhibition of Cellular Signals.” Institute of Science and Technology Austria, 2020. <a href=\"https://doi.org/10.15479/AT:ISTA:7680\">https://doi.org/10.15479/AT:ISTA:7680</a>.","ieee":"S. Kainrath, “Synthetic tools for optogenetic and chemogenetic inhibition of cellular signals,” Institute of Science and Technology Austria, 2020.","apa":"Kainrath, S. (2020). <i>Synthetic tools for optogenetic and chemogenetic inhibition of cellular signals</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT:ISTA:7680\">https://doi.org/10.15479/AT:ISTA:7680</a>","ista":"Kainrath S. 2020. Synthetic tools for optogenetic and chemogenetic inhibition of cellular signals. Institute of Science and Technology Austria.","short":"S. Kainrath, Synthetic Tools for Optogenetic and Chemogenetic Inhibition of Cellular Signals, Institute of Science and Technology Austria, 2020.","mla":"Kainrath, Stephanie. <i>Synthetic Tools for Optogenetic and Chemogenetic Inhibition of Cellular Signals</i>. Institute of Science and Technology Austria, 2020, doi:<a href=\"https://doi.org/10.15479/AT:ISTA:7680\">10.15479/AT:ISTA:7680</a>.","ama":"Kainrath S. Synthetic tools for optogenetic and chemogenetic inhibition of cellular signals. 2020. doi:<a href=\"https://doi.org/10.15479/AT:ISTA:7680\">10.15479/AT:ISTA:7680</a>"},"related_material":{"record":[{"relation":"dissertation_contains","status":"public","id":"1028"}]},"title":"Synthetic tools for optogenetic and chemogenetic inhibition of cellular signals","file":[{"embargo":"2021-10-30","creator":"stgingl","relation":"main_file","content_type":"application/pdf","access_level":"open_access","file_size":3268017,"file_name":"Thesis_without-signatures_PDFA.pdf","date_updated":"2021-10-31T23:30:05Z","checksum":"fb9a4468eb27be92690728e35c823796","date_created":"2020-04-28T11:19:21Z","file_id":"7692"},{"checksum":"f6c80ca97104a631a328cb79a2c53493","file_id":"7693","date_created":"2020-04-28T11:19:24Z","embargo_to":"open_access","date_updated":"2021-10-31T23:30:05Z","file_name":"Thesis_without signatures.docx","file_size":5167703,"access_level":"closed","relation":"source_file","content_type":"application/octet-stream","creator":"stgingl"}]},{"publisher":"Institute of Science and Technology Austria","year":"2019","language":[{"iso":"eng"}],"doi":"10.15479/at:ista:7132","type":"dissertation","oa":1,"_id":"7132","author":[{"first_name":"Catherine","full_name":"Mckenzie, Catherine","id":"3EEDE19A-F248-11E8-B48F-1D18A9856A87","last_name":"Mckenzie"}],"date_published":"2019-06-27T00:00:00Z","department":[{"_id":"HaJa"}],"date_created":"2019-11-27T09:07:14Z","ddc":["571","573"],"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","article_processing_charge":"No","file_date_updated":"2020-07-14T12:47:50Z","publication_identifier":{"issn":["2663-337X"]},"month":"06","supervisor":[{"first_name":"Harald L","full_name":"Janovjak, Harald L","orcid":"0000-0002-8023-9315","last_name":"Janovjak","id":"33BA6C30-F248-11E8-B48F-1D18A9856A87"}],"date_updated":"2024-03-25T23:30:11Z","status":"public","degree_awarded":"PhD","abstract":[{"lang":"eng","text":"A major challenge in neuroscience research is to dissect the circuits that orchestrate behavior in health and disease. Proteins from a wide range of non-mammalian species, such as microbial opsins, have been successfully transplanted to specific neuronal targets to override their natural communication patterns. The goal of our work is to manipulate synaptic communication in a manner that closely incorporates the functional intricacies of synapses by preserving temporal encoding (i.e. the firing pattern of the presynaptic neuron) and connectivity (i.e. target specific synapses rather than specific neurons). Our strategy to achieve this goal builds on the use of non-mammalian transplants to create a synthetic synapse. The mode of modulation comes from pre-synaptic uptake of a synthetic neurotransmitter (SN) into synaptic vesicles by means of a genetically targeted transporter selective for the SN. Upon natural vesicular release, exposure of the SN to the synaptic cleft will modify the post-synaptic potential through an orthogonal ligand gated ion channel. To achieve this goal we have functionally characterized a mixed cationic methionine-gated ion channel from Arabidopsis thaliana, designed a method to functionally characterize a synthetic transporter in isolated synaptic vesicles without the need for transgenic animals, identified and extracted multiple prokaryotic uptake systems that are substrate specific for methionine (Met), and established a primary/cell line co-culture system that would allow future combinatorial testing of this orthogonal transmitter-transporter-channel trifecta.\r\nSynthetic synapses will provide a unique opportunity to manipulate synaptic communication while maintaining the electrophysiological integrity of the pre-synaptic cell. In this way, information may be preserved that was generated in upstream circuits and that could be essential for concerted function and information processing."}],"publication_status":"published","oa_version":"Published Version","page":"95","day":"27","citation":{"ama":"Mckenzie C. Design and characterization of methods and biological components to realize synthetic neurotransmission. 2019. doi:<a href=\"https://doi.org/10.15479/at:ista:7132\">10.15479/at:ista:7132</a>","mla":"Mckenzie, Catherine. <i>Design and Characterization of Methods and Biological Components to Realize Synthetic Neurotransmission</i>. Institute of Science and Technology Austria, 2019, doi:<a href=\"https://doi.org/10.15479/at:ista:7132\">10.15479/at:ista:7132</a>.","apa":"Mckenzie, C. (2019). <i>Design and characterization of methods and biological components to realize synthetic neurotransmission</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/at:ista:7132\">https://doi.org/10.15479/at:ista:7132</a>","ista":"Mckenzie C. 2019. Design and characterization of methods and biological components to realize synthetic neurotransmission. Institute of Science and Technology Austria.","short":"C. Mckenzie, Design and Characterization of Methods and Biological Components to Realize Synthetic Neurotransmission, Institute of Science and Technology Austria, 2019.","ieee":"C. Mckenzie, “Design and characterization of methods and biological components to realize synthetic neurotransmission,” Institute of Science and Technology Austria, 2019.","chicago":"Mckenzie, Catherine. “Design and Characterization of Methods and Biological Components to Realize Synthetic Neurotransmission.” Institute of Science and Technology Austria, 2019. <a href=\"https://doi.org/10.15479/at:ista:7132\">https://doi.org/10.15479/at:ista:7132</a>."},"alternative_title":["ISTA Thesis"],"has_accepted_license":"1","related_material":{"record":[{"status":"public","id":"6266","relation":"old_edition"}]},"title":"Design and characterization of methods and biological components to realize synthetic neurotransmission","file":[{"checksum":"34d0fe0f6e0af97b5937205a3e350423","date_created":"2019-11-27T09:06:10Z","file_id":"7133","date_updated":"2020-07-14T12:47:50Z","file_name":"McKenzie PhD Thesis August 2018 - Corrected Final.docx","file_size":5054633,"access_level":"closed","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","relation":"source_file","creator":"dernst"},{"checksum":"140dfb5e3df7edca34f4b6fcc55d876f","date_created":"2019-11-27T09:06:10Z","file_id":"7134","date_updated":"2020-07-14T12:47:50Z","file_name":"McKenzie PhD Thesis August 2018 - Corrected Final.pdf","file_size":3231837,"access_level":"open_access","content_type":"application/pdf","relation":"main_file","creator":"dernst"}]},{"article_processing_charge":"No","ddc":["571","573"],"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","file_date_updated":"2021-02-11T11:17:16Z","date_created":"2019-04-09T14:13:39Z","department":[{"_id":"HaJa"}],"supervisor":[{"orcid":"0000-0002-8023-9315","last_name":"Janovjak","id":"33BA6C30-F248-11E8-B48F-1D18A9856A87","first_name":"Harald L","full_name":"Janovjak, Harald L"}],"month":"10","publication_identifier":{"issn":["2663-337X"]},"type":"dissertation","language":[{"iso":"eng"}],"doi":"10.15479/at:ista:th_1055","publisher":"Institute of Science and Technology Austria","year":"2018","date_published":"2018-10-31T00:00:00Z","author":[{"full_name":"Mckenzie, Catherine","first_name":"Catherine","last_name":"Mckenzie","id":"3EEDE19A-F248-11E8-B48F-1D18A9856A87"}],"oa":1,"_id":"6266","alternative_title":["ISTA Thesis"],"has_accepted_license":"1","pubrep_id":"1055","citation":{"chicago":"Mckenzie, Catherine. “Design and Characterization of Methods and Biological Components to Realize Synthetic Neurotransmission .” Institute of Science and Technology Austria, 2018. <a href=\"https://doi.org/10.15479/at:ista:th_1055\">https://doi.org/10.15479/at:ista:th_1055</a>.","ieee":"C. Mckenzie, “Design and characterization of methods and biological components to realize synthetic neurotransmission ,” Institute of Science and Technology Austria, 2018.","short":"C. Mckenzie, Design and Characterization of Methods and Biological Components to Realize Synthetic Neurotransmission , Institute of Science and Technology Austria, 2018.","mla":"Mckenzie, Catherine. <i>Design and Characterization of Methods and Biological Components to Realize Synthetic Neurotransmission </i>. Institute of Science and Technology Austria, 2018, doi:<a href=\"https://doi.org/10.15479/at:ista:th_1055\">10.15479/at:ista:th_1055</a>.","apa":"Mckenzie, C. (2018). <i>Design and characterization of methods and biological components to realize synthetic neurotransmission </i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/at:ista:th_1055\">https://doi.org/10.15479/at:ista:th_1055</a>","ista":"Mckenzie C. 2018. Design and characterization of methods and biological components to realize synthetic neurotransmission . Institute of Science and Technology Austria.","ama":"Mckenzie C. Design and characterization of methods and biological components to realize synthetic neurotransmission . 2018. doi:<a href=\"https://doi.org/10.15479/at:ista:th_1055\">10.15479/at:ista:th_1055</a>"},"related_material":{"record":[{"relation":"new_edition","id":"7132","status":"public"}]},"file":[{"file_name":"2018_Thesis_McKenzie.pdf","file_size":4906420,"date_created":"2019-04-09T14:12:40Z","checksum":"9d2c2dca04b00e485470c28b262af59a","file_id":"6267","date_updated":"2021-02-11T11:17:16Z","creator":"dernst","embargo":"2019-11-24","access_level":"open_access","relation":"main_file","content_type":"application/pdf"},{"access_level":"closed","relation":"source_file","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","creator":"dernst","date_created":"2019-04-09T14:12:40Z","file_id":"6268","checksum":"50b58c272899601bc6fd9642c4dc97f1","date_updated":"2020-07-14T12:47:25Z","embargo_to":"open_access","file_name":"2018_Thesis_McKenzie_source.docx","file_size":5053545}],"title":"Design and characterization of methods and biological components to realize synthetic neurotransmission ","status":"public","date_updated":"2023-09-07T13:02:37Z","page":"95","publication_status":"published","oa_version":"Published Version","day":"31","abstract":[{"lang":"eng","text":"A major challenge in neuroscience research is to dissect the circuits that orchestrate behavior in health and disease. Proteins from a wide range of non-mammalian species, such as microbial opsins, have been successfully transplanted to specific neuronal targets to override their natural communication patterns. The goal of our work is to manipulate synaptic communication in a manner that closely incorporates the functional intricacies of synapses by preserving temporal encoding (i.e. the firing pattern of the presynaptic neuron) and connectivity (i.e. target specific synapses rather than specific neurons). Our strategy to achieve this goal builds on the use of non-mammalian transplants to create a synthetic synapse. The mode of modulation comes from pre-synaptic uptake of a synthetic neurotransmitter (SN) into synaptic vesicles by means of a genetically targeted transporter selective for the SN. Upon natural vesicular release, exposure of the SN to the synaptic cleft will modify the post-synaptic potential through an orthogonal ligand gated ion channel. To achieve this goal we have functionally characterized a mixed cationic methionine-gated ion channel from Arabidopsis thaliana, designed a method to functionally characterize a synthetic transporter in isolated synaptic vesicles without the need for transgenic animals, identified and extracted multiple prokaryotic uptake systems that are substrate specific for methionine (Met), and established a primary/cell line co-culture system that would allow future combinatorial testing of this orthogonal transmitter-transporter-channel trifecta. Synthetic synapses will provide a unique opportunity to manipulate synaptic communication while maintaining the electrophysiological integrity of the pre-synaptic cell. In this way, information may be preserved that was generated in upstream circuits and that could be essential for concerted function and information processing. "}],"degree_awarded":"PhD"},{"file":[{"file_size":7012495,"file_name":"2018_THESIS_Gschaider-Reichhart_source.docx","date_updated":"2020-07-14T12:46:24Z","date_created":"2019-04-05T09:28:03Z","checksum":"697fa72ca36fb1b8ceabc133d58a73e5","file_id":"6222","creator":"dernst","relation":"source_file","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","access_level":"closed"},{"file_name":"2018_THESIS_Gschaider-Reichhart.pdf","file_size":6355280,"checksum":"58d7d1e9e58aeb7f061ab686b1d8a48c","date_created":"2019-04-05T09:28:03Z","file_id":"6223","date_updated":"2020-07-14T12:46:24Z","creator":"dernst","access_level":"open_access","relation":"main_file","content_type":"application/pdf"}],"title":"Optical and optogenetic control of proliferation and survival ","related_material":{"record":[{"id":"1441","status":"public","relation":"part_of_dissertation"},{"status":"public","id":"1678","relation":"part_of_dissertation"},{"relation":"part_of_dissertation","status":"public","id":"2084"},{"relation":"part_of_dissertation","status":"public","id":"1028"}]},"citation":{"chicago":"Gschaider-Reichhart, Eva. “Optical and Optogenetic Control of Proliferation and Survival .” Institute of Science and Technology Austria, 2018. <a href=\"https://doi.org/10.15479/AT:ISTA:th_913\">https://doi.org/10.15479/AT:ISTA:th_913</a>.","ieee":"E. Gschaider-Reichhart, “Optical and optogenetic control of proliferation and survival ,” Institute of Science and Technology Austria, 2018.","ista":"Gschaider-Reichhart E. 2018. Optical and optogenetic control of proliferation and survival . Institute of Science and Technology Austria.","short":"E. Gschaider-Reichhart, Optical and Optogenetic Control of Proliferation and Survival , Institute of Science and Technology Austria, 2018.","mla":"Gschaider-Reichhart, Eva. <i>Optical and Optogenetic Control of Proliferation and Survival </i>. Institute of Science and Technology Austria, 2018, doi:<a href=\"https://doi.org/10.15479/AT:ISTA:th_913\">10.15479/AT:ISTA:th_913</a>.","apa":"Gschaider-Reichhart, E. (2018). <i>Optical and optogenetic control of proliferation and survival </i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT:ISTA:th_913\">https://doi.org/10.15479/AT:ISTA:th_913</a>","ama":"Gschaider-Reichhart E. Optical and optogenetic control of proliferation and survival . 2018. doi:<a href=\"https://doi.org/10.15479/AT:ISTA:th_913\">10.15479/AT:ISTA:th_913</a>"},"pubrep_id":"913","has_accepted_license":"1","alternative_title":["ISTA Thesis"],"degree_awarded":"PhD","abstract":[{"text":"The aim of this thesis was the development of new strategies for optical and optogenetic control of proliferative and pro-survival signaling, and characterizing them from the molecular mechanism up to cellular effects. These new light-based methods have unique features, such as red light as an activator, or the avoidance of gene delivery, which enable to overcome current limitations, such as light delivery to target tissues and feasibility as therapeutic approach. A special focus was placed on implementing these new light-based approaches in pancreatic β-cells, as β-cells are the key players in diabetes and especially their loss in number negatively affects disease progression. Currently no treatment options are available to compensate the lack of functional β-cells in diabetic patients.\r\nIn a first approach, red-light-activated growth factor receptors, in particular receptor tyrosine kinases were engineered and characterized. Receptor activation with light allows spatio-temporal control compared to ligand-based activation, and especially red light exhibits deeper tissue penetration than other wavelengths of the visible spectrum. Red-light-activated receptor tyrosine kinases robustly activated major growth factor related signaling pathways with a high temporal resolution. Moreover, the remote activation of the proliferative MAPK/Erk pathway by red-light-activated receptor tyrosine kinases in a pancreatic β-cell line was also achieved, through one centimeter thick mouse tissue. Although red-light-activated receptor tyrosine kinases are particularly attractive for applications in animal models due to the deep tissue penetration of red light, a drawback, especially with regard to translation into humans, is the requirement of gene therapy.\r\nIn a second approach an endogenous light-sensitive mechanism was identified and its potential to promote proliferative and pro-survival signals was explored, towards light-based tissue regeneration without the need for gene transfer. Blue-green light illumination was found to be sufficient for the activation of proliferation and survival promoting signaling pathways in primary pancreatic murine and human islets. Blue-green light also led to an increase in proliferation of primary islet cells, an effect which was shown to be mostly β-cell specific in human islets. Moreover, it was demonstrated that this approach of pancreatic β-cell expansion did not have any negative effect on the β-cell function, in particular on their insulin secretion capacity. In contrast, a trend for enhanced insulin secretion under high glucose conditions after illumination was detected. In order to unravel the detailed characteristics of this endogenous light-sensitive mechanism, the precise light requirements were determined. In addition, the expression of light sensing proteins, OPN3 and rhodopsin, was detected. The observed effects were found to be independent of handling effects such as temperature differences and cytochrome c oxidase dependent ATP increase, but they were found to be enhanced through the knockout of OPN3. The exact mechanism of how islets cells sense light and the identity of the photoreceptor remains unknown.\r\nSummarized two new light-based systems with unique features were established that enable the activation of proliferative and pro-survival signaling pathways. While red-light-activated receptor tyrosine kinases open a new avenue for optogenetics research, by allowing non-invasive control of signaling in vivo, the identified endogenous light-sensitive mechanism has the potential to be the basis of a gene therapy-free therapeutical approach for light-based β-cell expansion.","lang":"eng"}],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"day":"08","oa_version":"Published Version","publication_status":"published","page":"107","date_updated":"2023-09-22T09:20:10Z","status":"public","publist_id":"7405","publication_identifier":{"issn":["2663-337X"]},"month":"01","supervisor":[{"first_name":"Harald L","full_name":"Janovjak, Harald L","last_name":"Janovjak","id":"33BA6C30-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8023-9315"}],"department":[{"_id":"HaJa"}],"date_created":"2018-12-11T11:46:22Z","file_date_updated":"2020-07-14T12:46:24Z","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","ddc":["571","570"],"article_processing_charge":"No","_id":"418","oa":1,"author":[{"id":"3FEE232A-F248-11E8-B48F-1D18A9856A87","last_name":"Gschaider-Reichhart","orcid":"0000-0002-7218-7738","full_name":"Gschaider-Reichhart, Eva","first_name":"Eva"}],"date_published":"2018-01-08T00:00:00Z","year":"2018","publisher":"Institute of Science and Technology Austria","doi":"10.15479/AT:ISTA:th_913","language":[{"iso":"eng"}],"type":"dissertation"},{"author":[{"id":"4863116E-F248-11E8-B48F-1D18A9856A87","last_name":"Morri","first_name":"Maurizio","full_name":"Morri, Maurizio"}],"degree_awarded":"PhD","_id":"1124","oa":1,"day":"01","date_published":"2016-03-01T00:00:00Z","oa_version":"Published Version","page":"129","publication_status":"published","language":[{"iso":"eng"}],"year":"2016","date_updated":"2023-09-07T11:43:03Z","publisher":"Institute of Science and Technology Austria","status":"public","type":"dissertation","month":"03","publist_id":"6236","publication_identifier":{"issn":["2663-337X"]},"supervisor":[{"orcid":"0000-0002-8023-9315","last_name":"Janovjak","id":"33BA6C30-F248-11E8-B48F-1D18A9856A87","full_name":"Janovjak, Harald L","first_name":"Harald L"}],"file":[{"creator":"dernst","access_level":"closed","content_type":"application/pdf","relation":"main_file","file_name":"MORRI_PhD_thesis_FINALPLUSSIGNATURES (2).pdf","file_size":4785167,"date_created":"2019-08-13T10:50:00Z","file_id":"6812","checksum":"b439803ac0827cdddd56562a54e3b53b","date_updated":"2019-08-13T10:50:00Z"},{"date_updated":"2021-02-22T11:42:06Z","success":1,"file_id":"9180","date_created":"2021-02-22T11:42:06Z","checksum":"dd4136247fe472e7d47880ec68ac8de0","file_size":4495669,"file_name":"2016_MORRI_Thesis.pdf","relation":"main_file","content_type":"application/pdf","access_level":"open_access","creator":"dernst"}],"title":"Optical functionalization of human class A orphan G-protein coupled receptors","date_created":"2018-12-11T11:50:17Z","department":[{"_id":"HaJa"}],"citation":{"chicago":"Morri, Maurizio. “Optical Functionalization of Human Class A Orphan G-Protein Coupled Receptors.” Institute of Science and Technology Austria, 2016.","ieee":"M. Morri, “Optical functionalization of human class A orphan G-protein coupled receptors,” Institute of Science and Technology Austria, 2016.","short":"M. Morri, Optical Functionalization of Human Class A Orphan G-Protein Coupled Receptors, Institute of Science and Technology Austria, 2016.","ista":"Morri M. 2016. Optical functionalization of human class A orphan G-protein coupled receptors. Institute of Science and Technology Austria.","mla":"Morri, Maurizio. <i>Optical Functionalization of Human Class A Orphan G-Protein Coupled Receptors</i>. Institute of Science and Technology Austria, 2016.","apa":"Morri, M. (2016). <i>Optical functionalization of human class A orphan G-protein coupled receptors</i>. Institute of Science and Technology Austria.","ama":"Morri M. Optical functionalization of human class A orphan G-protein coupled receptors. 2016."},"file_date_updated":"2021-02-22T11:42:06Z","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","article_processing_charge":"No","ddc":["570"],"has_accepted_license":"1","alternative_title":["ISTA Thesis"]}]