---
_id: '12122'
abstract:
- lang: eng
  text: Centrosomes play a crucial role during immune cell interactions and initiation
    of the immune response. In proliferating cells, centrosome numbers are tightly
    controlled and generally limited to one in G1 and two prior to mitosis. Defects
    in regulating centrosome numbers have been associated with cell transformation
    and tumorigenesis. Here, we report the emergence of extra centrosomes in leukocytes
    during immune activation. Upon antigen encounter, dendritic cells pass through
    incomplete mitosis and arrest in the subsequent G1 phase leading to tetraploid
    cells with accumulated centrosomes. In addition, cell stimulation increases expression
    of polo-like kinase 2, resulting in diploid cells with two centrosomes in G1-arrested
    cells. During cell migration, centrosomes tightly cluster and act as functional
    microtubule-organizing centers allowing for increased persistent locomotion along
    gradients of chemotactic cues. Moreover, dendritic cells with extra centrosomes
    display enhanced secretion of inflammatory cytokines and optimized T cell responses.
    Together, these results demonstrate a previously unappreciated role of extra centrosomes
    for regular cell and tissue homeostasis.
acknowledgement: "We thank Markéta Dalecká and Irena Krejzová for their support with
  FIB-SEM imaging, the Imaging Methods Core Facility at BIOCEV supported by the Ministry
  of Education, Youth and Sports Czech Republic (Large RI Project LM2018129 Czech-BioImaging),
  and European Regional Development Fund (project No. CZ.02.1.01/0.0/0.0/18_046/0016045)
  for their support with obtaining imaging data presented in this paper. The authors
  further thank Andreas Villunger, Florian Gärtner, Frank Bradke, and Sarah Förster
  for helpful discussions; Andy Zielinski for help with statistics; and Björn Weiershausen
  for assisting with figure illustration.\r\n\r\nThis work was funded by a fellowship
  of the Ministry of Innovation, Science and Research of North-Rhine-Westphalia (AZ:
  421-8.03.03.02-137069) to E. Kiermaier and the Deutsche Forschungsgemeinschaft (German
  Research Foundation) under Germany’s Excellence Strategy – EXC 2151 – 390873048.
  R. Hauschild was funded by grant number 2020-225401 from the Chan Zuckerberg Initiative
  Donor-Advised Fund, an advised fund of Silicon Valley Community Foundation. M. Hons
  is supported by Czech Science Foundation GACR 20-24603Y and Charles University PRIMUS/20/MED/013."
article_number: e202107134
article_processing_charge: No
article_type: original
author:
- first_name: Ann-Kathrin
  full_name: Weier, Ann-Kathrin
  last_name: Weier
- first_name: Mirka
  full_name: Homrich, Mirka
  last_name: Homrich
- first_name: Stephanie
  full_name: Ebbinghaus, Stephanie
  last_name: Ebbinghaus
- first_name: Pavel
  full_name: Juda, Pavel
  last_name: Juda
- first_name: Eliška
  full_name: Miková, Eliška
  last_name: Miková
- first_name: Robert
  full_name: Hauschild, Robert
  id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
  last_name: Hauschild
  orcid: 0000-0001-9843-3522
- first_name: Lili
  full_name: Zhang, Lili
  last_name: Zhang
- first_name: Thomas
  full_name: Quast, Thomas
  last_name: Quast
- first_name: Elvira
  full_name: Mass, Elvira
  last_name: Mass
- first_name: Andreas
  full_name: Schlitzer, Andreas
  last_name: Schlitzer
- first_name: Waldemar
  full_name: Kolanus, Waldemar
  last_name: Kolanus
- first_name: Sven
  full_name: Burgdorf, Sven
  last_name: Burgdorf
- first_name: Oliver J.
  full_name: Gruß, Oliver J.
  last_name: Gruß
- first_name: Miroslav
  full_name: Hons, Miroslav
  last_name: Hons
- first_name: Stefan
  full_name: Wieser, Stefan
  last_name: Wieser
- first_name: Eva
  full_name: Kiermaier, Eva
  last_name: Kiermaier
citation:
  ama: Weier A-K, Homrich M, Ebbinghaus S, et al. Multiple centrosomes enhance migration
    and immune cell effector functions of mature dendritic cells. <i>Journal of Cell
    Biology</i>. 2022;221(12). doi:<a href="https://doi.org/10.1083/jcb.202107134">10.1083/jcb.202107134</a>
  apa: Weier, A.-K., Homrich, M., Ebbinghaus, S., Juda, P., Miková, E., Hauschild,
    R., … Kiermaier, E. (2022). Multiple centrosomes enhance migration and immune
    cell effector functions of mature dendritic cells. <i>Journal of Cell Biology</i>.
    Rockefeller University Press. <a href="https://doi.org/10.1083/jcb.202107134">https://doi.org/10.1083/jcb.202107134</a>
  chicago: Weier, Ann-Kathrin, Mirka Homrich, Stephanie Ebbinghaus, Pavel Juda, Eliška
    Miková, Robert Hauschild, Lili Zhang, et al. “Multiple Centrosomes Enhance Migration
    and Immune Cell Effector Functions of Mature Dendritic Cells.” <i>Journal of Cell
    Biology</i>. Rockefeller University Press, 2022. <a href="https://doi.org/10.1083/jcb.202107134">https://doi.org/10.1083/jcb.202107134</a>.
  ieee: A.-K. Weier <i>et al.</i>, “Multiple centrosomes enhance migration and immune
    cell effector functions of mature dendritic cells,” <i>Journal of Cell Biology</i>,
    vol. 221, no. 12. Rockefeller University Press, 2022.
  ista: Weier A-K, Homrich M, Ebbinghaus S, Juda P, Miková E, Hauschild R, Zhang L,
    Quast T, Mass E, Schlitzer A, Kolanus W, Burgdorf S, Gruß OJ, Hons M, Wieser S,
    Kiermaier E. 2022. Multiple centrosomes enhance migration and immune cell effector
    functions of mature dendritic cells. Journal of Cell Biology. 221(12), e202107134.
  mla: Weier, Ann-Kathrin, et al. “Multiple Centrosomes Enhance Migration and Immune
    Cell Effector Functions of Mature Dendritic Cells.” <i>Journal of Cell Biology</i>,
    vol. 221, no. 12, e202107134, Rockefeller University Press, 2022, doi:<a href="https://doi.org/10.1083/jcb.202107134">10.1083/jcb.202107134</a>.
  short: A.-K. Weier, M. Homrich, S. Ebbinghaus, P. Juda, E. Miková, R. Hauschild,
    L. Zhang, T. Quast, E. Mass, A. Schlitzer, W. Kolanus, S. Burgdorf, O.J. Gruß,
    M. Hons, S. Wieser, E. Kiermaier, Journal of Cell Biology 221 (2022).
date_created: 2023-01-12T12:01:09Z
date_published: 2022-12-05T00:00:00Z
date_updated: 2023-08-16T11:29:12Z
day: '05'
ddc:
- '570'
department:
- _id: Bio
doi: 10.1083/jcb.202107134
external_id:
  isi:
  - '000932941400001'
  pmid:
  - '36214847 '
file:
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intvolume: '       221'
isi: 1
issue: '12'
keyword:
- Cell Biology
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: c08e9ad1-5a5b-11eb-8a69-9d1cf3b07473
  grant_number: CZI01
  name: Tools for automation and feedback microscopy
publication: Journal of Cell Biology
publication_identifier:
  eissn:
  - 1540-8140
  issn:
  - 0021-9525
publication_status: published
publisher: Rockefeller University Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: Multiple centrosomes enhance migration and immune cell effector functions of
  mature dendritic cells
tmp:
  image: /images/cc_by_nc_sa.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC
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  short: CC BY-NC-SA (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 221
year: '2022'
...