---
_id: '14644'
abstract:
- lang: eng
  text: Transcription by RNA polymerase II (Pol II) can be repressed by noncoding
    RNA, including the human RNA Alu. However, the mechanism by which endogenous RNAs
    repress transcription remains unclear. Here we present cryo-electron microscopy
    structures of Pol II bound to Alu RNA, which reveal that Alu RNA mimics how DNA
    and RNA bind to Pol II during transcription elongation. Further, we show how domains
    of the general transcription factor TFIIF affect complex dynamics and control
    repressive activity. Together, we reveal how a non-coding RNA can regulate mammalian
    gene expression.
acknowledged_ssus:
- _id: LifeSc
- _id: EM-Fac
- _id: PreCl
acknowledgement: "We thank B. Kaczmarek and other members of the Bernecky lab for
  helpful discussions. We thank V.-V. Hodirnau for SerialEM data collection and support
  with EPU data collection. We thank D. Slade for the wild type TFIIF expression\r\nplasmid.
  We thank N. Thompson and R. Burgess for the 8WG16 hybridoma cell line. We thank
  C. Plaschka and M. Loose for critical reading of the manuscript. This work was supported
  by Austrian Science Fund (FWF) grant P34185. This research was further supported
  by the Scientific Service Units (SSU) of IST Austria through resources provided
  by the Lab Support Facility (LSF), Electron Microscopy Facility (EMF), Scientific
  Computing (SciComp), and the Preclinical Facility (PCF)."
article_processing_charge: No
author:
- first_name: Katarina
  full_name: Tluckova, Katarina
  id: 4AC7D980-F248-11E8-B48F-1D18A9856A87
  last_name: Tluckova
- first_name: Anita P
  full_name: Testa Salmazo, Anita P
  id: 41F1F098-F248-11E8-B48F-1D18A9856A87
  last_name: Testa Salmazo
- first_name: Carrie A
  full_name: Bernecky, Carrie A
  id: 2CB9DFE2-F248-11E8-B48F-1D18A9856A87
  last_name: Bernecky
  orcid: 0000-0003-0893-7036
citation:
  ama: Tluckova K, Testa Salmazo AP, Bernecky C. Mechanism of mammalian transcriptional
    repression by noncoding RNA. doi:<a href="https://doi.org/10.15479/AT:ISTA:14644">10.15479/AT:ISTA:14644</a>
  apa: Tluckova, K., Testa Salmazo, A. P., &#38; Bernecky, C. (n.d.). Mechanism of
    mammalian transcriptional repression by noncoding RNA. Institute of Science and
    Technology Austria. <a href="https://doi.org/10.15479/AT:ISTA:14644">https://doi.org/10.15479/AT:ISTA:14644</a>
  chicago: Tluckova, Katarina, Anita P Testa Salmazo, and Carrie Bernecky. “Mechanism
    of Mammalian Transcriptional Repression by Noncoding RNA.” Institute of Science
    and Technology Austria, n.d. <a href="https://doi.org/10.15479/AT:ISTA:14644">https://doi.org/10.15479/AT:ISTA:14644</a>.
  ieee: K. Tluckova, A. P. Testa Salmazo, and C. Bernecky, “Mechanism of mammalian
    transcriptional repression by noncoding RNA.” Institute of Science and Technology
    Austria.
  ista: Tluckova K, Testa Salmazo AP, Bernecky C. Mechanism of mammalian transcriptional
    repression by noncoding RNA. <a href="https://doi.org/10.15479/AT:ISTA:14644">10.15479/AT:ISTA:14644</a>.
  mla: Tluckova, Katarina, et al. <i>Mechanism of Mammalian Transcriptional Repression
    by Noncoding RNA</i>. Institute of Science and Technology Austria, doi:<a href="https://doi.org/10.15479/AT:ISTA:14644">10.15479/AT:ISTA:14644</a>.
  short: K. Tluckova, A.P. Testa Salmazo, C. Bernecky, (n.d.).
date_created: 2023-12-04T14:51:00Z
date_published: 2023-12-05T00:00:00Z
date_updated: 2023-12-05T10:37:28Z
day: '05'
ddc:
- '572'
department:
- _id: CaBe
doi: 10.15479/AT:ISTA:14644
file:
- access_level: open_access
  checksum: c45608cb97ee36d7b50ba518db8e07b0
  content_type: application/pdf
  creator: dernst
  date_created: 2023-12-05T10:37:02Z
  date_updated: 2023-12-05T10:37:02Z
  file_id: '14646'
  file_name: 2023_Tluckova_etal_REx.pdf
  file_size: 4892920
  relation: main_file
  success: 1
file_date_updated: 2023-12-05T10:37:02Z
has_accepted_license: '1'
language:
- iso: eng
month: '12'
oa: 1
oa_version: Submitted Version
project:
- _id: c08a6700-5a5b-11eb-8a69-82a722b2bc30
  grant_number: P34185
  name: Regulation of mammalian transcription by noncoding RNA
publication_status: submitted
publisher: Institute of Science and Technology Austria
status: public
title: Mechanism of mammalian transcriptional repression by noncoding RNA
tmp:
  image: /images/cc_by_nc.png
  legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
  short: CC BY-NC (4.0)
type: preprint
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2023'
...