---
_id: '8721'
abstract:
- lang: eng
  text: Spontaneously arising channels that transport the phytohormone auxin provide
    positional cues for self-organizing aspects of plant development such as flexible
    vasculature regeneration or its patterning during leaf venation. The auxin canalization
    hypothesis proposes a feedback between auxin signaling and transport as the underlying
    mechanism, but molecular players await discovery. We identified part of the machinery
    that routes auxin transport. The auxin-regulated receptor CAMEL (Canalization-related
    Auxin-regulated Malectin-type RLK) together with CANAR (Canalization-related Receptor-like
    kinase) interact with and phosphorylate PIN auxin transporters. camel and canar
    mutants are impaired in PIN1 subcellular trafficking and auxin-mediated PIN polarization,
    which macroscopically manifests as defects in leaf venation and vasculature regeneration
    after wounding. The CAMEL-CANAR receptor complex is part of the auxin feedback
    that coordinates polarization of individual cells during auxin canalization.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: 'We acknowledge M. Glanc and Y. Zhang for providing entryclones;
  Vienna Biocenter Core Facilities (VBCF) for recombinantprotein production and purification;
  Vienna Biocenter Massspectrometry Facility, Bioimaging, and Life Science Facilities
  at IST Austria and Proteomics Core Facility CEITEC for a great assistance.Funding:This
  project received funding from the European Research Council (ERC) under the European
  Union’s Horizon 2020 research and innovation program (grant agreement 742985) and
  Austrian Science Fund (FWF): I 3630-B25 to J.F.and by grants from the Austrian Academy
  of Science through the Gregor Mendel Institute (Y.B.) and the Austrian Agency for
  International Cooperation in Education and Research (D.D.); the Netherlands Organization
  for Scientific Research (NWO; VIDI-864.13.001) (W.S.); the Research Foundation–Flanders
  (FWO;Odysseus II G0D0515N) and a European Research Council grant (ERC; StG TORPEDO;
  714055) to B.D.R., B.Y., and E.M.; and the Hertha Firnberg Programme postdoctoral
  fellowship (T-947) from the FWF Austrian Science Fund to E.S.-L.; J.H. is the recipient
  of a DOC Fellowship of the Austrian Academy of Sciences at IST Austria.'
article_processing_charge: No
article_type: original
author:
- first_name: Jakub
  full_name: Hajny, Jakub
  id: 4800CC20-F248-11E8-B48F-1D18A9856A87
  last_name: Hajny
  orcid: 0000-0003-2140-7195
- first_name: Tomas
  full_name: Prat, Tomas
  id: 3DA3BFEE-F248-11E8-B48F-1D18A9856A87
  last_name: Prat
- first_name: N
  full_name: Rydza, N
  last_name: Rydza
- first_name: Lesia
  full_name: Rodriguez Solovey, Lesia
  id: 3922B506-F248-11E8-B48F-1D18A9856A87
  last_name: Rodriguez Solovey
  orcid: 0000-0002-7244-7237
- first_name: Shutang
  full_name: Tan, Shutang
  id: 2DE75584-F248-11E8-B48F-1D18A9856A87
  last_name: Tan
  orcid: 0000-0002-0471-8285
- first_name: Inge
  full_name: Verstraeten, Inge
  id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
  last_name: Verstraeten
  orcid: 0000-0001-7241-2328
- first_name: David
  full_name: Domjan, David
  id: C684CD7A-257E-11EA-9B6F-D8588B4F947F
  last_name: Domjan
  orcid: 0000-0003-2267-106X
- first_name: E
  full_name: Mazur, E
  last_name: Mazur
- first_name: E
  full_name: Smakowska-Luzan, E
  last_name: Smakowska-Luzan
- first_name: W
  full_name: Smet, W
  last_name: Smet
- first_name: E
  full_name: Mor, E
  last_name: Mor
- first_name: J
  full_name: Nolf, J
  last_name: Nolf
- first_name: B
  full_name: Yang, B
  last_name: Yang
- first_name: W
  full_name: Grunewald, W
  last_name: Grunewald
- first_name: Gergely
  full_name: Molnar, Gergely
  id: 34F1AF46-F248-11E8-B48F-1D18A9856A87
  last_name: Molnar
- first_name: Y
  full_name: Belkhadir, Y
  last_name: Belkhadir
- first_name: B
  full_name: De Rybel, B
  last_name: De Rybel
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
citation:
  ama: Hajny J, Prat T, Rydza N, et al. Receptor kinase module targets PIN-dependent
    auxin transport during canalization. <i>Science</i>. 2020;370(6516):550-557. doi:<a
    href="https://doi.org/10.1126/science.aba3178">10.1126/science.aba3178</a>
  apa: Hajny, J., Prat, T., Rydza, N., Rodriguez Solovey, L., Tan, S., Verstraeten,
    I., … Friml, J. (2020). Receptor kinase module targets PIN-dependent auxin transport
    during canalization. <i>Science</i>. American Association for the Advancement
    of Science. <a href="https://doi.org/10.1126/science.aba3178">https://doi.org/10.1126/science.aba3178</a>
  chicago: Hajny, Jakub, Tomas Prat, N Rydza, Lesia Rodriguez Solovey, Shutang Tan,
    Inge Verstraeten, David Domjan, et al. “Receptor Kinase Module Targets PIN-Dependent
    Auxin Transport during Canalization.” <i>Science</i>. American Association for
    the Advancement of Science, 2020. <a href="https://doi.org/10.1126/science.aba3178">https://doi.org/10.1126/science.aba3178</a>.
  ieee: J. Hajny <i>et al.</i>, “Receptor kinase module targets PIN-dependent auxin
    transport during canalization,” <i>Science</i>, vol. 370, no. 6516. American Association
    for the Advancement of Science, pp. 550–557, 2020.
  ista: Hajny J, Prat T, Rydza N, Rodriguez Solovey L, Tan S, Verstraeten I, Domjan
    D, Mazur E, Smakowska-Luzan E, Smet W, Mor E, Nolf J, Yang B, Grunewald W, Molnar
    G, Belkhadir Y, De Rybel B, Friml J. 2020. Receptor kinase module targets PIN-dependent
    auxin transport during canalization. Science. 370(6516), 550–557.
  mla: Hajny, Jakub, et al. “Receptor Kinase Module Targets PIN-Dependent Auxin Transport
    during Canalization.” <i>Science</i>, vol. 370, no. 6516, American Association
    for the Advancement of Science, 2020, pp. 550–57, doi:<a href="https://doi.org/10.1126/science.aba3178">10.1126/science.aba3178</a>.
  short: J. Hajny, T. Prat, N. Rydza, L. Rodriguez Solovey, S. Tan, I. Verstraeten,
    D. Domjan, E. Mazur, E. Smakowska-Luzan, W. Smet, E. Mor, J. Nolf, B. Yang, W.
    Grunewald, G. Molnar, Y. Belkhadir, B. De Rybel, J. Friml, Science 370 (2020)
    550–557.
date_created: 2020-11-02T10:04:46Z
date_published: 2020-10-30T00:00:00Z
date_updated: 2023-09-05T12:02:35Z
day: '30'
department:
- _id: JiFr
doi: 10.1126/science.aba3178
ec_funded: 1
external_id:
  isi:
  - '000583031800041'
  pmid:
  - '33122378'
intvolume: '       370'
isi: 1
issue: '6516'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://europepmc.org/article/MED/33122378#free-full-text
month: '10'
oa: 1
oa_version: Published Version
page: 550-557
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '742985'
  name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: I03630
  name: Molecular mechanisms of endocytic cargo recognition in plants
- _id: 2699E3D2-B435-11E9-9278-68D0E5697425
  grant_number: '25239'
  name: Cell surface receptor complexes for PIN polarity and auxin-mediated development
publication: Science
publication_identifier:
  eissn:
  - 1095-9203
  issn:
  - 0036-8075
publication_status: published
publisher: American Association for the Advancement of Science
quality_controlled: '1'
related_material:
  link:
  - description: News on IST Homepage
    relation: press_release
    url: https://ist.ac.at/en/news/molecular-compass-for-cell-orientation/
scopus_import: '1'
status: public
title: Receptor kinase module targets PIN-dependent auxin transport during canalization
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 370
year: '2020'
...
---
_id: '7500'
abstract:
- lang: eng
  text: "Plant survival depends on vascular tissues, which originate in a self‐organizing
    manner as strands of cells co‐directionally transporting the plant hormone auxin.
    The latter phenomenon (also known as auxin canalization) is classically hypothesized
    to be regulated by auxin itself via the effect of this hormone on the polarity
    of its own intercellular transport. Correlative observations supported this concept,
    but molecular insights remain limited.\r\nIn the current study, we established
    an experimental system based on the model Arabidopsis thaliana, which exhibits
    auxin transport channels and formation of vasculature strands in response to local
    auxin application.\r\nOur methodology permits the genetic analysis of auxin canalization
    under controllable experimental conditions. By utilizing this opportunity, we
    confirmed the dependence of auxin canalization on a PIN‐dependent auxin transport
    and nuclear, TIR1/AFB‐mediated auxin signaling. We also show that leaf venation
    and auxin‐mediated PIN repolarization in the root require TIR1/AFB signaling.\r\nFurther
    studies based on this experimental system are likely to yield better understanding
    of the mechanisms underlying auxin transport polarization in other developmental
    contexts."
acknowledgement: We thank Mark Estelle, José M. Alonso and the Arabidopsis Stock Centre
  for providing seeds. We acknowledge the core facility CELLIM of CEITEC supported
  by the MEYS CR (LM2015062 Czech‐BioImaging) and Plant Sciences Core Facility of
  CEITEC Masaryk University for help in generating essential data. This project received
  funding from the European Research Council (ERC) under the European Union's Horizon
  2020 research and innovation program (grant agreement no. 742985) and the Czech
  Science Foundation GAČR (GA13‐40637S and GA18‐26981S) to JF. JH is the recipient
  of a DOC Fellowship of the Austrian Academy of Sciences at the Institute of Science
  and Technology. The authors declare no competing interests.
article_processing_charge: No
article_type: original
author:
- first_name: E
  full_name: Mazur, E
  last_name: Mazur
- first_name: Ivan
  full_name: Kulik, Ivan
  id: F0AB3FCE-02D1-11E9-BD0E-99399A5D3DEB
  last_name: Kulik
- first_name: Jakub
  full_name: Hajny, Jakub
  id: 4800CC20-F248-11E8-B48F-1D18A9856A87
  last_name: Hajny
  orcid: 0000-0003-2140-7195
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
citation:
  ama: Mazur E, Kulik I, Hajny J, Friml J. Auxin canalization and vascular tissue
    formation by TIR1/AFB-mediated auxin signaling in arabidopsis. <i>New Phytologist</i>.
    2020;226(5):1375-1383. doi:<a href="https://doi.org/10.1111/nph.16446">10.1111/nph.16446</a>
  apa: Mazur, E., Kulik, I., Hajny, J., &#38; Friml, J. (2020). Auxin canalization
    and vascular tissue formation by TIR1/AFB-mediated auxin signaling in arabidopsis.
    <i>New Phytologist</i>. Wiley. <a href="https://doi.org/10.1111/nph.16446">https://doi.org/10.1111/nph.16446</a>
  chicago: Mazur, E, Ivan Kulik, Jakub Hajny, and Jiří Friml. “Auxin Canalization
    and Vascular Tissue Formation by TIR1/AFB-Mediated Auxin Signaling in Arabidopsis.”
    <i>New Phytologist</i>. Wiley, 2020. <a href="https://doi.org/10.1111/nph.16446">https://doi.org/10.1111/nph.16446</a>.
  ieee: E. Mazur, I. Kulik, J. Hajny, and J. Friml, “Auxin canalization and vascular
    tissue formation by TIR1/AFB-mediated auxin signaling in arabidopsis,” <i>New
    Phytologist</i>, vol. 226, no. 5. Wiley, pp. 1375–1383, 2020.
  ista: Mazur E, Kulik I, Hajny J, Friml J. 2020. Auxin canalization and vascular
    tissue formation by TIR1/AFB-mediated auxin signaling in arabidopsis. New Phytologist.
    226(5), 1375–1383.
  mla: Mazur, E., et al. “Auxin Canalization and Vascular Tissue Formation by TIR1/AFB-Mediated
    Auxin Signaling in Arabidopsis.” <i>New Phytologist</i>, vol. 226, no. 5, Wiley,
    2020, pp. 1375–83, doi:<a href="https://doi.org/10.1111/nph.16446">10.1111/nph.16446</a>.
  short: E. Mazur, I. Kulik, J. Hajny, J. Friml, New Phytologist 226 (2020) 1375–1383.
date_created: 2020-02-18T10:03:47Z
date_published: 2020-06-01T00:00:00Z
date_updated: 2024-03-25T23:30:21Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1111/nph.16446
ec_funded: 1
external_id:
  isi:
  - '000514939700001'
  pmid:
  - '31971254'
file:
- access_level: open_access
  checksum: 17de728b0205979feb95ce663ba918c2
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  creator: dernst
  date_created: 2020-11-20T09:32:10Z
  date_updated: 2020-11-20T09:32:10Z
  file_id: '8781'
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file_date_updated: 2020-11-20T09:32:10Z
has_accepted_license: '1'
intvolume: '       226'
isi: 1
issue: '5'
language:
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month: '06'
oa: 1
oa_version: Published Version
page: 1375-1383
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '742985'
  name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 2699E3D2-B435-11E9-9278-68D0E5697425
  grant_number: '25239'
  name: Cell surface receptor complexes for PIN polarity and auxin-mediated development
publication: New Phytologist
publication_identifier:
  eissn:
  - 1469-8137
  issn:
  - 0028-646x
publication_status: published
publisher: Wiley
quality_controlled: '1'
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    relation: dissertation_contains
    status: public
status: public
title: Auxin canalization and vascular tissue formation by TIR1/AFB-mediated auxin
  signaling in arabidopsis
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 226
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...