@article{8721,
  abstract     = {Spontaneously arising channels that transport the phytohormone auxin provide positional cues for self-organizing aspects of plant development such as flexible vasculature regeneration or its patterning during leaf venation. The auxin canalization hypothesis proposes a feedback between auxin signaling and transport as the underlying mechanism, but molecular players await discovery. We identified part of the machinery that routes auxin transport. The auxin-regulated receptor CAMEL (Canalization-related Auxin-regulated Malectin-type RLK) together with CANAR (Canalization-related Receptor-like kinase) interact with and phosphorylate PIN auxin transporters. camel and canar mutants are impaired in PIN1 subcellular trafficking and auxin-mediated PIN polarization, which macroscopically manifests as defects in leaf venation and vasculature regeneration after wounding. The CAMEL-CANAR receptor complex is part of the auxin feedback that coordinates polarization of individual cells during auxin canalization.},
  author       = {Hajny, Jakub and Prat, Tomas and Rydza, N and Rodriguez Solovey, Lesia and Tan, Shutang and Verstraeten, Inge and Domjan, David and Mazur, E and Smakowska-Luzan, E and Smet, W and Mor, E and Nolf, J and Yang, B and Grunewald, W and Molnar, Gergely and Belkhadir, Y and De Rybel, B and Friml, Jiří},
  issn         = {1095-9203},
  journal      = {Science},
  number       = {6516},
  pages        = {550--557},
  publisher    = {American Association for the Advancement of Science},
  title        = {{Receptor kinase module targets PIN-dependent auxin transport during canalization}},
  doi          = {10.1126/science.aba3178},
  volume       = {370},
  year         = {2020},
}

@article{7500,
  abstract     = {Plant survival depends on vascular tissues, which originate in a self‐organizing manner as strands of cells co‐directionally transporting the plant hormone auxin. The latter phenomenon (also known as auxin canalization) is classically hypothesized to be regulated by auxin itself via the effect of this hormone on the polarity of its own intercellular transport. Correlative observations supported this concept, but molecular insights remain limited.
In the current study, we established an experimental system based on the model Arabidopsis thaliana, which exhibits auxin transport channels and formation of vasculature strands in response to local auxin application.
Our methodology permits the genetic analysis of auxin canalization under controllable experimental conditions. By utilizing this opportunity, we confirmed the dependence of auxin canalization on a PIN‐dependent auxin transport and nuclear, TIR1/AFB‐mediated auxin signaling. We also show that leaf venation and auxin‐mediated PIN repolarization in the root require TIR1/AFB signaling.
Further studies based on this experimental system are likely to yield better understanding of the mechanisms underlying auxin transport polarization in other developmental contexts.},
  author       = {Mazur, E and Kulik, Ivan and Hajny, Jakub and Friml, Jiří},
  issn         = {1469-8137},
  journal      = {New Phytologist},
  number       = {5},
  pages        = {1375--1383},
  publisher    = {Wiley},
  title        = {{Auxin canalization and vascular tissue formation by TIR1/AFB-mediated auxin signaling in arabidopsis}},
  doi          = {10.1111/nph.16446},
  volume       = {226},
  year         = {2020},
}