@article{10713,
  abstract     = {Cells migrate through crowded microenvironments within tissues during normal development, immune response, and cancer metastasis. Although migration through pores and tracks in the extracellular matrix (ECM) has been well studied, little is known about cellular traversal into confining cell-dense tissues. We find that embryonic tissue invasion by Drosophila macrophages requires division of an epithelial ectodermal cell at the site of entry. Dividing ectodermal cells disassemble ECM attachment formed by integrin-mediated focal adhesions next to mesodermal cells, allowing macrophages to move their nuclei ahead and invade between two immediately adjacent tissues. Invasion efficiency depends on division frequency, but reduction of adhesion strength allows macrophage entry independently of division. This work demonstrates that tissue dynamics can regulate cellular infiltration.},
  author       = {Akhmanova, Maria and Emtenani, Shamsi and Krueger, Daniel and György, Attila and Pereira Guarda, Mariana and Vlasov, Mikhail and Vlasov, Fedor and Akopian, Andrei and Ratheesh, Aparna and De Renzis, Stefano and Siekhaus, Daria E},
  issn         = {0036-8075},
  journal      = {Science},
  number       = {6591},
  pages        = {394--396},
  publisher    = {American Association for the Advancement of Science},
  title        = {{Cell division in tissues enables macrophage infiltration}},
  doi          = {10.1126/science.abj0425},
  volume       = {376},
  year         = {2022},
}

@article{10918,
  abstract     = {Cellular metabolism must adapt to changing demands to enable homeostasis. During immune responses or cancer metastasis, cells leading migration into challenging environments require an energy boost, but what controls this capacity is unclear. Here, we study a previously uncharacterized nuclear protein, Atossa (encoded by CG9005), which supports macrophage invasion into the germband of Drosophila by controlling cellular metabolism. First, nuclear Atossa increases mRNA levels of Porthos, a DEAD-box protein, and of two metabolic enzymes, lysine-α-ketoglutarate reductase (LKR/SDH) and NADPH glyoxylate reductase (GR/HPR), thus enhancing mitochondrial bioenergetics. Then Porthos supports ribosome assembly and thereby raises the translational efficiency of a subset of mRNAs, including those affecting mitochondrial functions, the electron transport chain, and metabolism. Mitochondrial respiration measurements, metabolomics, and live imaging indicate that Atossa and Porthos power up OxPhos and energy production to promote the forging of a path into tissues by leading macrophages. Since many crucial physiological responses require increases in mitochondrial energy output, this previously undescribed genetic program may modulate a wide range of cellular behaviors.},
  author       = {Emtenani, Shamsi and Martin, Elliot T and György, Attila and Bicher, Julia and Genger, Jakob-Wendelin and Köcher, Thomas and Akhmanova, Maria and Pereira Guarda, Mariana and Roblek, Marko and Bergthaler, Andreas and Hurd, Thomas R and Rangan, Prashanth and Siekhaus, Daria E},
  issn         = {1460-2075},
  journal      = {The Embo Journal},
  publisher    = {Embo Press},
  title        = {{Macrophage mitochondrial bioenergetics and tissue invasion are boosted by an Atossa-Porthos axis in Drosophila}},
  doi          = {10.15252/embj.2021109049},
  volume       = {41},
  year         = {2022},
}

@article{7180,
  abstract     = {Arabidopsis PIN2 protein directs transport of the phytohormone auxin from the root tip into the root elongation zone. Variation in hormone transport, which depends on a delicate interplay between PIN2 sorting to and from polar plasma membrane domains, determines root growth. By employing a constitutively degraded version of PIN2, we identify brassinolides as antagonists of PIN2 endocytosis. This response does not require de novo protein synthesis, but involves early events in canonical brassinolide signaling. Brassinolide-controlled adjustments in PIN2 sorting and intracellular distribution governs formation of a lateral PIN2 gradient in gravistimulated roots, coinciding with adjustments in auxin signaling and directional root growth. Strikingly, simulations indicate that PIN2 gradient formation is no prerequisite for root bending but rather dampens asymmetric auxin flow and signaling. Crosstalk between brassinolide signaling and endocytic PIN2 sorting, thus, appears essential for determining the rate of gravity-induced root curvature via attenuation of differential cell elongation.},
  author       = {Retzer, Katarzyna and Akhmanova, Maria and Konstantinova, Nataliia and Malínská, Kateřina and Leitner, Johannes and Petrášek, Jan and Luschnig, Christian},
  issn         = {20411723},
  journal      = {Nature Communications},
  publisher    = {Springer Nature},
  title        = {{Brassinosteroid signaling delimits root gravitropism via sorting of the Arabidopsis PIN2 auxin transporter}},
  doi          = {10.1038/s41467-019-13543-1},
  volume       = {10},
  year         = {2019},
}