---
_id: '14846'
abstract:
- lang: eng
  text: Contraction and flow of the actin cell cortex have emerged as a common principle
    by which cells reorganize their cytoplasm and take shape. However, how these cortical
    flows interact with adjacent cytoplasmic components, changing their form and localization,
    and how this affects cytoplasmic organization and cell shape remains unclear.
    Here we show that in ascidian oocytes, the cooperative activities of cortical
    actomyosin flows and deformation of the adjacent mitochondria-rich myoplasm drive
    oocyte cytoplasmic reorganization and shape changes following fertilization. We
    show that vegetal-directed cortical actomyosin flows, established upon oocyte
    fertilization, lead to both the accumulation of cortical actin at the vegetal
    pole of the zygote and compression and local buckling of the adjacent elastic
    solid-like myoplasm layer due to friction forces generated at their interface.
    Once cortical flows have ceased, the multiple myoplasm buckles resolve into one
    larger buckle, which again drives the formation of the contraction pole—a protuberance
    of the zygote’s vegetal pole where maternal mRNAs accumulate. Thus, our findings
    reveal a mechanism where cortical actomyosin network flows determine cytoplasmic
    reorganization and cell shape by deforming adjacent cytoplasmic components through
    friction forces.
acknowledged_ssus:
- _id: EM-Fac
- _id: Bio
- _id: NanoFab
acknowledgement: We would like to thank A. McDougall, E. Hannezo and the Heisenberg
  lab for fruitful discussions and reagents. We also thank E. Munro for the iMyo-YFP
  and Bra>iMyo-mScarlet constructs. This research was supported by the Scientific
  Service Units of the Institute of Science and Technology Austria through resources
  provided by the Electron Microscopy Facility, Imaging and Optics Facility and the
  Nanofabrication Facility. This work was supported by a Joint Project Grant from
  the FWF (I 3601-B27).
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Silvia
  full_name: Caballero Mancebo, Silvia
  id: 2F1E1758-F248-11E8-B48F-1D18A9856A87
  last_name: Caballero Mancebo
  orcid: 0000-0002-5223-3346
- first_name: Rushikesh
  full_name: Shinde, Rushikesh
  last_name: Shinde
- first_name: Madison
  full_name: Bolger-Munro, Madison
  id: 516F03FA-93A3-11EA-A7C5-D6BE3DDC885E
  last_name: Bolger-Munro
  orcid: 0000-0002-8176-4824
- first_name: Matilda
  full_name: Peruzzo, Matilda
  id: 3F920B30-F248-11E8-B48F-1D18A9856A87
  last_name: Peruzzo
  orcid: 0000-0002-3415-4628
- first_name: Gregory
  full_name: Szep, Gregory
  id: 4BFB7762-F248-11E8-B48F-1D18A9856A87
  last_name: Szep
- first_name: Irene
  full_name: Steccari, Irene
  id: 2705C766-9FE2-11EA-B224-C6773DDC885E
  last_name: Steccari
- first_name: David
  full_name: Labrousse Arias, David
  id: CD573DF4-9ED3-11E9-9D77-3223E6697425
  last_name: Labrousse Arias
- first_name: Vanessa
  full_name: Zheden, Vanessa
  id: 39C5A68A-F248-11E8-B48F-1D18A9856A87
  last_name: Zheden
  orcid: 0000-0002-9438-4783
- first_name: Jack
  full_name: Merrin, Jack
  id: 4515C308-F248-11E8-B48F-1D18A9856A87
  last_name: Merrin
  orcid: 0000-0001-5145-4609
- first_name: Andrew
  full_name: Callan-Jones, Andrew
  last_name: Callan-Jones
- first_name: Raphaël
  full_name: Voituriez, Raphaël
  last_name: Voituriez
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
citation:
  ama: Caballero Mancebo S, Shinde R, Bolger-Munro M, et al. Friction forces determine
    cytoplasmic reorganization and shape changes of ascidian oocytes upon fertilization.
    <i>Nature Physics</i>. 2024. doi:<a href="https://doi.org/10.1038/s41567-023-02302-1">10.1038/s41567-023-02302-1</a>
  apa: Caballero Mancebo, S., Shinde, R., Bolger-Munro, M., Peruzzo, M., Szep, G.,
    Steccari, I., … Heisenberg, C.-P. J. (2024). Friction forces determine cytoplasmic
    reorganization and shape changes of ascidian oocytes upon fertilization. <i>Nature
    Physics</i>. Springer Nature. <a href="https://doi.org/10.1038/s41567-023-02302-1">https://doi.org/10.1038/s41567-023-02302-1</a>
  chicago: Caballero Mancebo, Silvia, Rushikesh Shinde, Madison Bolger-Munro, Matilda
    Peruzzo, Gregory Szep, Irene Steccari, David Labrousse Arias, et al. “Friction
    Forces Determine Cytoplasmic Reorganization and Shape Changes of Ascidian Oocytes
    upon Fertilization.” <i>Nature Physics</i>. Springer Nature, 2024. <a href="https://doi.org/10.1038/s41567-023-02302-1">https://doi.org/10.1038/s41567-023-02302-1</a>.
  ieee: S. Caballero Mancebo <i>et al.</i>, “Friction forces determine cytoplasmic
    reorganization and shape changes of ascidian oocytes upon fertilization,” <i>Nature
    Physics</i>. Springer Nature, 2024.
  ista: Caballero Mancebo S, Shinde R, Bolger-Munro M, Peruzzo M, Szep G, Steccari
    I, Labrousse Arias D, Zheden V, Merrin J, Callan-Jones A, Voituriez R, Heisenberg
    C-PJ. 2024. Friction forces determine cytoplasmic reorganization and shape changes
    of ascidian oocytes upon fertilization. Nature Physics.
  mla: Caballero Mancebo, Silvia, et al. “Friction Forces Determine Cytoplasmic Reorganization
    and Shape Changes of Ascidian Oocytes upon Fertilization.” <i>Nature Physics</i>,
    Springer Nature, 2024, doi:<a href="https://doi.org/10.1038/s41567-023-02302-1">10.1038/s41567-023-02302-1</a>.
  short: S. Caballero Mancebo, R. Shinde, M. Bolger-Munro, M. Peruzzo, G. Szep, I.
    Steccari, D. Labrousse Arias, V. Zheden, J. Merrin, A. Callan-Jones, R. Voituriez,
    C.-P.J. Heisenberg, Nature Physics (2024).
date_created: 2024-01-21T23:00:57Z
date_published: 2024-01-09T00:00:00Z
date_updated: 2024-03-05T09:33:38Z
day: '09'
department:
- _id: CaHe
- _id: JoFi
- _id: MiSi
- _id: EM-Fac
- _id: NanoFab
doi: 10.1038/s41567-023-02302-1
has_accepted_license: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1038/s41567-023-02302-1
month: '01'
oa: 1
oa_version: Published Version
project:
- _id: 2646861A-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: I03601
  name: Control of embryonic cleavage pattern
publication: Nature Physics
publication_identifier:
  eissn:
  - 1745-2481
  issn:
  - 1745-2473
publication_status: epub_ahead
publisher: Springer Nature
quality_controlled: '1'
related_material:
  link:
  - description: News on ISTA Website
    relation: press_release
    url: https://ista.ac.at/en/news/stranger-than-friction-a-force-initiating-life/
scopus_import: '1'
status: public
title: Friction forces determine cytoplasmic reorganization and shape changes of ascidian
  oocytes upon fertilization
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2024'
...
---
_id: '10606'
abstract:
- lang: eng
  text: Cell division orientation is thought to result from a competition between
    cell geometry and polarity domains controlling the position of the mitotic spindle
    during mitosis. Depending on the level of cell shape anisotropy or the strength
    of the polarity domain, one dominates the other and determines the orientation
    of the spindle. Whether and how such competition is also at work to determine
    unequal cell division (UCD), producing daughter cells of different size, remains
    unclear. Here, we show that cell geometry and polarity domains cooperate, rather
    than compete, in positioning the cleavage plane during UCDs in early ascidian
    embryos. We found that the UCDs and their orientation at the ascidian third cleavage
    rely on the spindle tilting in an anisotropic cell shape, and cortical polarity
    domains exerting different effects on spindle astral microtubules. By systematically
    varying mitotic cell shape, we could modulate the effect of attractive and repulsive
    polarity domains and consequently generate predicted daughter cell size asymmetries
    and position. We therefore propose that the spindle position during UCD is set
    by the combined activities of cell geometry and polarity domains, where cell geometry
    modulates the effect of cortical polarity domain(s).
acknowledged_ssus:
- _id: NanoFab
- _id: Bio
acknowledgement: 'We thank members of the Heisenberg and McDougall groups for technical
  advice and discussion. We are grateful to the Bioimaging and Nanofabrication facilities
  of IST Austria and the Imaging Platform (PIM) and animal facility (CRB) of Institut
  de la Mer de Villefranche (IMEV), which is supported by EMBRC-France, whose French
  state funds are managed by the ANR within the Investments of the Future program
  under reference ANR-10-INBS-0, for continuous support. This work was supported by
  a collaborative grant from the French Government funding agency Agence National
  de la Recherche to McDougall (ANR ''MorCell'': ANR-17-CE 13-0028) and the Austrian
  Science Fund to Heisenberg (FWF: I 3601-B27).'
article_number: e75639
article_processing_charge: No
article_type: original
author:
- first_name: Benoit G
  full_name: Godard, Benoit G
  id: 33280250-F248-11E8-B48F-1D18A9856A87
  last_name: Godard
- first_name: Remi
  full_name: Dumollard, Remi
  last_name: Dumollard
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
- first_name: Alex
  full_name: Mcdougall, Alex
  last_name: Mcdougall
citation:
  ama: Godard BG, Dumollard R, Heisenberg C-PJ, Mcdougall A. Combined effect of cell
    geometry and polarity domains determines the orientation of unequal division.
    <i>eLife</i>. 2021;10. doi:<a href="https://doi.org/10.7554/eLife.75639">10.7554/eLife.75639</a>
  apa: Godard, B. G., Dumollard, R., Heisenberg, C.-P. J., &#38; Mcdougall, A. (2021).
    Combined effect of cell geometry and polarity domains determines the orientation
    of unequal division. <i>ELife</i>. eLife Sciences Publications. <a href="https://doi.org/10.7554/eLife.75639">https://doi.org/10.7554/eLife.75639</a>
  chicago: Godard, Benoit G, Remi Dumollard, Carl-Philipp J Heisenberg, and Alex Mcdougall.
    “Combined Effect of Cell Geometry and Polarity Domains Determines the Orientation
    of Unequal Division.” <i>ELife</i>. eLife Sciences Publications, 2021. <a href="https://doi.org/10.7554/eLife.75639">https://doi.org/10.7554/eLife.75639</a>.
  ieee: B. G. Godard, R. Dumollard, C.-P. J. Heisenberg, and A. Mcdougall, “Combined
    effect of cell geometry and polarity domains determines the orientation of unequal
    division,” <i>eLife</i>, vol. 10. eLife Sciences Publications, 2021.
  ista: Godard BG, Dumollard R, Heisenberg C-PJ, Mcdougall A. 2021. Combined effect
    of cell geometry and polarity domains determines the orientation of unequal division.
    eLife. 10, e75639.
  mla: Godard, Benoit G., et al. “Combined Effect of Cell Geometry and Polarity Domains
    Determines the Orientation of Unequal Division.” <i>ELife</i>, vol. 10, e75639,
    eLife Sciences Publications, 2021, doi:<a href="https://doi.org/10.7554/eLife.75639">10.7554/eLife.75639</a>.
  short: B.G. Godard, R. Dumollard, C.-P.J. Heisenberg, A. Mcdougall, ELife 10 (2021).
date_created: 2022-01-09T23:01:26Z
date_published: 2021-12-21T00:00:00Z
date_updated: 2023-08-17T06:32:44Z
day: '21'
ddc:
- '570'
department:
- _id: CaHe
doi: 10.7554/eLife.75639
external_id:
  isi:
  - '000733610100001'
file:
- access_level: open_access
  checksum: 759c7a873d554c48a6639e6350746ca6
  content_type: application/pdf
  creator: alisjak
  date_created: 2022-01-10T09:40:37Z
  date_updated: 2022-01-10T09:40:37Z
  file_id: '10611'
  file_name: 2021_eLife_Godard.pdf
  file_size: 7769934
  relation: main_file
  success: 1
file_date_updated: 2022-01-10T09:40:37Z
has_accepted_license: '1'
intvolume: '        10'
isi: 1
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
project:
- _id: 2646861A-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: I03601
  name: Control of embryonic cleavage pattern
publication: eLife
publication_identifier:
  eissn:
  - 2050-084X
publication_status: published
publisher: eLife Sciences Publications
quality_controlled: '1'
scopus_import: '1'
status: public
title: Combined effect of cell geometry and polarity domains determines the orientation
  of unequal division
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 10
year: '2021'
...
---
_id: '7227'
abstract:
- lang: eng
  text: Gastrulation entails specification and formation of three embryonic germ layers—ectoderm,
    mesoderm and endoderm—thereby establishing the basis for the future body plan.
    In zebrafish embryos, germ layer specification occurs during blastula and early
    gastrula stages (Ho & Kimmel, 1993), a period when the main morphogenetic movements
    underlying gastrulation are initiated. Hence, the signals driving progenitor cell
    fate specification, such as Nodal ligands from the TGF-β family, also play key
    roles in regulating germ layer progenitor cell segregation (Carmany-Rampey & Schier,
    2001; David & Rosa, 2001; Feldman et al., 2000; Gritsman et al., 1999; Keller
    et al., 2008). In this review, we summarize and discuss the main signaling pathways
    involved in germ layer progenitor cell fate specification and segregation, specifically
    focusing on recent advances in understanding the interplay between mesoderm and
    endoderm specification and the internalization movements at the onset of zebrafish
    gastrulation.
acknowledgement: We thank Alexandra Schauer, Nicoletta Petridou and Feyza Nur Arslan
  for comments on the manuscript. Research in the Heisenberg laboratory is supported
  by an ERC Advanced Grant (MECSPEC 742573), ANR/FWF (I03601) and FWF/DFG (I03196)
  International Cooperation Grants. D. Pinheiro acknowledges a fellowship from EMBO
  ALTF (850-2017) and is currently supported by HFSP LTF (LT000429/2018-L2).
alternative_title:
- Current Topics in Developmental Biology
article_processing_charge: No
author:
- first_name: Diana C
  full_name: Nunes Pinheiro, Diana C
  id: 2E839F16-F248-11E8-B48F-1D18A9856A87
  last_name: Nunes Pinheiro
  orcid: 0000-0003-4333-7503
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
citation:
  ama: 'Nunes Pinheiro DC, Heisenberg C-PJ. Zebrafish gastrulation: Putting fate in
    motion. In: <i>Gastrulation: From Embryonic Pattern to Form</i>. Vol 136. Elsevier;
    2020:343-375. doi:<a href="https://doi.org/10.1016/bs.ctdb.2019.10.009">10.1016/bs.ctdb.2019.10.009</a>'
  apa: 'Nunes Pinheiro, D. C., &#38; Heisenberg, C.-P. J. (2020). Zebrafish gastrulation:
    Putting fate in motion. In <i>Gastrulation: From Embryonic Pattern to Form</i>
    (Vol. 136, pp. 343–375). Elsevier. <a href="https://doi.org/10.1016/bs.ctdb.2019.10.009">https://doi.org/10.1016/bs.ctdb.2019.10.009</a>'
  chicago: 'Nunes Pinheiro, Diana C, and Carl-Philipp J Heisenberg. “Zebrafish Gastrulation:
    Putting Fate in Motion.” In <i>Gastrulation: From Embryonic Pattern to Form</i>,
    136:343–75. Elsevier, 2020. <a href="https://doi.org/10.1016/bs.ctdb.2019.10.009">https://doi.org/10.1016/bs.ctdb.2019.10.009</a>.'
  ieee: 'D. C. Nunes Pinheiro and C.-P. J. Heisenberg, “Zebrafish gastrulation: Putting
    fate in motion,” in <i>Gastrulation: From Embryonic Pattern to Form</i>, vol.
    136, Elsevier, 2020, pp. 343–375.'
  ista: 'Nunes Pinheiro DC, Heisenberg C-PJ. 2020.Zebrafish gastrulation: Putting
    fate in motion. In: Gastrulation: From Embryonic Pattern to Form. Current Topics
    in Developmental Biology, vol. 136, 343–375.'
  mla: 'Nunes Pinheiro, Diana C., and Carl-Philipp J. Heisenberg. “Zebrafish Gastrulation:
    Putting Fate in Motion.” <i>Gastrulation: From Embryonic Pattern to Form</i>,
    vol. 136, Elsevier, 2020, pp. 343–75, doi:<a href="https://doi.org/10.1016/bs.ctdb.2019.10.009">10.1016/bs.ctdb.2019.10.009</a>.'
  short: 'D.C. Nunes Pinheiro, C.-P.J. Heisenberg, in:, Gastrulation: From Embryonic
    Pattern to Form, Elsevier, 2020, pp. 343–375.'
date_created: 2020-01-05T23:00:46Z
date_published: 2020-06-01T00:00:00Z
date_updated: 2023-09-06T14:54:36Z
day: '01'
department:
- _id: CaHe
doi: 10.1016/bs.ctdb.2019.10.009
ec_funded: 1
external_id:
  isi:
  - '000611830600013'
  pmid:
  - '31959295'
intvolume: '       136'
isi: 1
language:
- iso: eng
month: '06'
oa_version: None
page: 343-375
pmid: 1
project:
- _id: 260F1432-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '742573'
  name: Interaction and feedback between cell mechanics and fate specification in
    vertebrate gastrulation
- _id: 2646861A-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: I03601
  name: Control of embryonic cleavage pattern
- _id: 2608FC64-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: I03196
  name: Control of epithelial cell layer spreading in zebrafish
- _id: 266BC5CE-B435-11E9-9278-68D0E5697425
  grant_number: LT000429
  name: Coordination of mesendoderm fate specification and internalization during
    zebrafish gastrulation
- _id: 26520D1E-B435-11E9-9278-68D0E5697425
  grant_number: ALTF 850-2017
  name: Coordination of mesendoderm cell fate specification and internalization during
    zebrafish gastrulation
publication: 'Gastrulation: From Embryonic Pattern to Form'
publication_identifier:
  issn:
  - '00702153'
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Zebrafish gastrulation: Putting fate in motion'
type: book_chapter
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 136
year: '2020'
...