[{"type":"journal_article","date_updated":"2021-01-12T06:49:06Z","_id":"1209","publisher":"American Society for Biochemistry and Molecular Biology","doi":"10.1074/jbc.M116.735142","quality_controlled":"1","main_file_link":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5114416/","open_access":"1"}],"page":"24657 - 24675","publist_id":"6139","year":"2016","acknowledgement":"J.A.S supported in part by a Medical Research D.G.Council UK Ph.D. fellowship.\r\nThis work was supported in part by European Union's 2020 Research and Innovation Program under Grant 701309. \r\n","date_published":"2016-11-18T00:00:00Z","ec_funded":1,"project":[{"_id":"2593EBD6-B435-11E9-9278-68D0E5697425","name":"Atomic-Resolution Structures of Mitochondrial Respiratory Chain Supercomplexes (FEBS)"},{"name":"Atomic-Resolution Structures of Mitochondrial Respiratory Chain Supercomplexes (H2020)","grant_number":"701309","call_identifier":"H2020","_id":"2590DB08-B435-11E9-9278-68D0E5697425"}],"status":"public","publication":"Journal of Biological Chemistry","date_created":"2018-12-11T11:50:44Z","volume":291,"title":"Purification of ovine respiratory complex i results in a highly active and stable preparation","oa_version":"Submitted Version","author":[{"first_name":"James A","orcid":"0000-0002-9864-3586","full_name":"Letts, James A","id":"322DA418-F248-11E8-B48F-1D18A9856A87","last_name":"Letts"},{"first_name":"Gianluca","full_name":"Degliesposti, Gianluca","last_name":"Degliesposti"},{"first_name":"Karol","last_name":"Fiedorczuk","full_name":"Fiedorczuk, Karol","id":"5BFF67CE-02D1-11E9-B11A-A5A4D7DFFFD0"},{"first_name":"Mark","last_name":"Skehel","full_name":"Skehel, Mark"},{"orcid":"0000-0002-0977-7989","first_name":"Leonid A","last_name":"Sazanov","id":"338D39FE-F248-11E8-B48F-1D18A9856A87","full_name":"Sazanov, Leonid A"}],"day":"18","scopus_import":1,"publication_status":"published","intvolume":"       291","abstract":[{"lang":"eng","text":"NADH-ubiquinone oxidoreductase (complex I) is the largest (∼1 MDa) and the least characterized complex of the mitochondrial electron transport chain. Because of the ease of sample availability, previous work has focused almost exclusively on bovine complex I. However, only medium resolution structural analyses of this complex have been reported. Working with other mammalian complex I homologues is a potential approach for overcoming these limitations. Due to the inherent difficulty of expressing large membrane protein complexes, screening of complex I homologues is limited to large mammals reared for human consumption. The high sequence identity among these available sources may preclude the benefits of screening. Here, we report the characterization of complex I purified from Ovis aries (ovine) heart mitochondria. All 44 unique subunits of the intact complex were identified by mass spectrometry. We identified differences in the subunit composition of subcomplexes of ovine complex I as compared with bovine, suggesting differential stability of inter-subunit interactions within the complex. Furthermore, the 42-kDa subunit, which is easily lost from the bovine enzyme, remains tightly bound to ovine complex I. Additionally, we developed a novel purification protocol for highly active and stable mitochondrial complex I using the branched-chain detergent lauryl maltose neopentyl glycol. Our data demonstrate that, although closely related, significant differences exist between the biochemical properties of complex I prepared from ovine and bovine mitochondria and that ovine complex I represents a suitable alternative target for further structural studies. "}],"department":[{"_id":"LeSa"}],"month":"11","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","issue":"47","citation":{"ieee":"J. A. Letts, G. Degliesposti, K. Fiedorczuk, M. Skehel, and L. A. Sazanov, “Purification of ovine respiratory complex i results in a highly active and stable preparation,” <i>Journal of Biological Chemistry</i>, vol. 291, no. 47. American Society for Biochemistry and Molecular Biology, pp. 24657–24675, 2016.","short":"J.A. Letts, G. Degliesposti, K. Fiedorczuk, M. Skehel, L.A. Sazanov, Journal of Biological Chemistry 291 (2016) 24657–24675.","ama":"Letts JA, Degliesposti G, Fiedorczuk K, Skehel M, Sazanov LA. Purification of ovine respiratory complex i results in a highly active and stable preparation. <i>Journal of Biological Chemistry</i>. 2016;291(47):24657-24675. doi:<a href=\"https://doi.org/10.1074/jbc.M116.735142\">10.1074/jbc.M116.735142</a>","apa":"Letts, J. A., Degliesposti, G., Fiedorczuk, K., Skehel, M., &#38; Sazanov, L. A. (2016). Purification of ovine respiratory complex i results in a highly active and stable preparation. <i>Journal of Biological Chemistry</i>. American Society for Biochemistry and Molecular Biology. <a href=\"https://doi.org/10.1074/jbc.M116.735142\">https://doi.org/10.1074/jbc.M116.735142</a>","mla":"Letts, James A., et al. “Purification of Ovine Respiratory Complex i Results in a Highly Active and Stable Preparation.” <i>Journal of Biological Chemistry</i>, vol. 291, no. 47, American Society for Biochemistry and Molecular Biology, 2016, pp. 24657–75, doi:<a href=\"https://doi.org/10.1074/jbc.M116.735142\">10.1074/jbc.M116.735142</a>.","chicago":"Letts, James A, Gianluca Degliesposti, Karol Fiedorczuk, Mark Skehel, and Leonid A Sazanov. “Purification of Ovine Respiratory Complex i Results in a Highly Active and Stable Preparation.” <i>Journal of Biological Chemistry</i>. American Society for Biochemistry and Molecular Biology, 2016. <a href=\"https://doi.org/10.1074/jbc.M116.735142\">https://doi.org/10.1074/jbc.M116.735142</a>.","ista":"Letts JA, Degliesposti G, Fiedorczuk K, Skehel M, Sazanov LA. 2016. Purification of ovine respiratory complex i results in a highly active and stable preparation. Journal of Biological Chemistry. 291(47), 24657–24675."},"language":[{"iso":"eng"}],"oa":1},{"abstract":[{"lang":"eng","text":"Mitochondrial complex I (also known as NADH:ubiquinone oxidoreductase) contributes to cellular energy production by transferring electrons from NADH to ubiquinone coupled to proton translocation across the membrane. It is the largest protein assembly of the respiratory chain with a total mass of 970 kilodaltons. Here we present a nearly complete atomic structure of ovine (Ovis aries) mitochondrial complex I at 3.9 Å resolution, solved by cryo-electron microscopy with cross-linking and mass-spectrometry mapping experiments. All 14 conserved core subunits and 31 mitochondria-specific supernumerary subunits are resolved within the L-shaped molecule. The hydrophilic matrix arm comprises flavin mononucleotide and 8 iron-sulfur clusters involved in electron transfer, and the membrane arm contains 78 transmembrane helices, mostly contributed by antiporter-like subunits involved in proton translocation. Supernumerary subunits form an interlinked, stabilizing shell around the conserved core. Tightly bound lipids (including cardiolipins) further stabilize interactions between the hydrophobic subunits. Subunits with possible regulatory roles contain additional cofactors, NADPH and two phosphopantetheine molecules, which are shown to be involved in inter-subunit interactions. We observe two different conformations of the complex, which may be related to the conformationally driven coupling mechanism and to the active-deactive transition of the enzyme. Our structure provides insight into the mechanism, assembly, maturation and dysfunction of mitochondrial complex I, and allows detailed molecular analysis of disease-causing mutations."}],"intvolume":"       538","publication_status":"published","title":"Atomic structure of the entire mammalian mitochondrial complex i","oa_version":"Submitted Version","day":"20","scopus_import":1,"author":[{"first_name":"Karol","last_name":"Fiedorczuk","full_name":"Fiedorczuk, Karol","id":"5BFF67CE-02D1-11E9-B11A-A5A4D7DFFFD0"},{"last_name":"Letts","full_name":"Letts, James A","id":"322DA418-F248-11E8-B48F-1D18A9856A87","first_name":"James A","orcid":"0000-0002-9864-3586"},{"full_name":"Degliesposti, Gianluca","last_name":"Degliesposti","first_name":"Gianluca"},{"first_name":"Karol","full_name":"Kaszuba, Karol","id":"3FDF9472-F248-11E8-B48F-1D18A9856A87","last_name":"Kaszuba"},{"full_name":"Skehel, Mark","last_name":"Skehel","first_name":"Mark"},{"last_name":"Sazanov","full_name":"Sazanov, Leonid A","id":"338D39FE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0977-7989","first_name":"Leonid A"}],"date_created":"2018-12-11T11:50:49Z","article_type":"original","volume":538,"language":[{"iso":"eng"}],"oa":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"short":"K. Fiedorczuk, J.A. Letts, G. Degliesposti, K. Kaszuba, M. Skehel, L.A. Sazanov, Nature 538 (2016) 406–410.","ieee":"K. Fiedorczuk, J. A. Letts, G. Degliesposti, K. Kaszuba, M. Skehel, and L. A. Sazanov, “Atomic structure of the entire mammalian mitochondrial complex i,” <i>Nature</i>, vol. 538, no. 7625. Nature Publishing Group, pp. 406–410, 2016.","ama":"Fiedorczuk K, Letts JA, Degliesposti G, Kaszuba K, Skehel M, Sazanov LA. Atomic structure of the entire mammalian mitochondrial complex i. <i>Nature</i>. 2016;538(7625):406-410. doi:<a href=\"https://doi.org/10.1038/nature19794\">10.1038/nature19794</a>","mla":"Fiedorczuk, Karol, et al. “Atomic Structure of the Entire Mammalian Mitochondrial Complex I.” <i>Nature</i>, vol. 538, no. 7625, Nature Publishing Group, 2016, pp. 406–10, doi:<a href=\"https://doi.org/10.1038/nature19794\">10.1038/nature19794</a>.","apa":"Fiedorczuk, K., Letts, J. A., Degliesposti, G., Kaszuba, K., Skehel, M., &#38; Sazanov, L. A. (2016). Atomic structure of the entire mammalian mitochondrial complex i. <i>Nature</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/nature19794\">https://doi.org/10.1038/nature19794</a>","ista":"Fiedorczuk K, Letts JA, Degliesposti G, Kaszuba K, Skehel M, Sazanov LA. 2016. Atomic structure of the entire mammalian mitochondrial complex i. Nature. 538(7625), 406–410.","chicago":"Fiedorczuk, Karol, James A Letts, Gianluca Degliesposti, Karol Kaszuba, Mark Skehel, and Leonid A Sazanov. “Atomic Structure of the Entire Mammalian Mitochondrial Complex I.” <i>Nature</i>. Nature Publishing Group, 2016. <a href=\"https://doi.org/10.1038/nature19794\">https://doi.org/10.1038/nature19794</a>."},"issue":"7625","month":"10","department":[{"_id":"LeSa"}],"page":"406 - 410","quality_controlled":"1","main_file_link":[{"open_access":"1","url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5164932/"}],"publisher":"Nature Publishing Group","article_processing_charge":"No","doi":"10.1038/nature19794","type":"journal_article","_id":"1226","date_updated":"2021-01-12T06:49:13Z","publication":"Nature","status":"public","project":[{"_id":"2593EBD6-B435-11E9-9278-68D0E5697425","name":"Atomic-Resolution Structures of Mitochondrial Respiratory Chain Supercomplexes (FEBS)"},{"_id":"2590DB08-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","grant_number":"701309","name":"Atomic-Resolution Structures of Mitochondrial Respiratory Chain Supercomplexes (H2020)"}],"date_published":"2016-10-20T00:00:00Z","ec_funded":1,"pmid":1,"external_id":{"pmid":["27595392"]},"year":"2016","publist_id":"6108"},{"quality_controlled":"1","publication_status":"published","intvolume":"       537","abstract":[{"lang":"eng","text":"Mitochondrial electron transport chain complexes are organized into supercomplexes responsible for carrying out cellular respiration. Here we present three architectures of mammalian (ovine) supercomplexes determined by cryo-electron microscopy. We identify two distinct arrangements of supercomplex CICIII 2 CIV (the respirasome) - a major 'tight' form and a minor 'loose' form (resolved at the resolution of 5.8 Å and 6.7 Å, respectively), which may represent different stages in supercomplex assembly or disassembly. We have also determined an architecture of supercomplex CICIII 2 at 7.8 Å resolution. All observed density can be attributed to the known 80 subunits of the individual complexes, including 132 transmembrane helices. The individual complexes form tight interactions that vary between the architectures, with complex IV subunit COX7a switching contact from complex III to complex I. The arrangement of active sites within the supercomplex may help control reactive oxygen species production. To our knowledge, these are the first complete architectures of the dominant, physiologically relevant state of the electron transport chain."}],"page":"644 - 648","date_created":"2018-12-11T11:50:51Z","type":"journal_article","_id":"1232","volume":537,"date_updated":"2021-01-12T06:49:16Z","title":"The architecture of respiratory supercomplexes","oa_version":"None","publisher":"Nature Publishing Group","scopus_import":1,"day":"29","doi":"10.1038/nature19774","author":[{"orcid":"0000-0002-9864-3586","first_name":"James A","full_name":"Letts, James A","id":"322DA418-F248-11E8-B48F-1D18A9856A87","last_name":"Letts"},{"id":"5BFF67CE-02D1-11E9-B11A-A5A4D7DFFFD0","full_name":"Fiedorczuk, Karol","last_name":"Fiedorczuk","first_name":"Karol"},{"first_name":"Leonid A","orcid":"0000-0002-0977-7989","last_name":"Sazanov","id":"338D39FE-F248-11E8-B48F-1D18A9856A87","full_name":"Sazanov, Leonid A"}],"user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","date_published":"2016-09-29T00:00:00Z","acknowledgement":"We thank the MRC LMB Cambridge for the use of the Titan Krios microscope. Data processing was performed using the IST high-performance computer cluster. J.A.L. holds a long-term fellowship from FEBS. K.F. is partially funded by a MRC UK PhD fellowship.","citation":{"short":"J.A. Letts, K. Fiedorczuk, L.A. Sazanov, Nature 537 (2016) 644–648.","ieee":"J. A. Letts, K. Fiedorczuk, and L. A. Sazanov, “The architecture of respiratory supercomplexes,” <i>Nature</i>, vol. 537, no. 7622. Nature Publishing Group, pp. 644–648, 2016.","ama":"Letts JA, Fiedorczuk K, Sazanov LA. The architecture of respiratory supercomplexes. <i>Nature</i>. 2016;537(7622):644-648. doi:<a href=\"https://doi.org/10.1038/nature19774\">10.1038/nature19774</a>","mla":"Letts, James A., et al. “The Architecture of Respiratory Supercomplexes.” <i>Nature</i>, vol. 537, no. 7622, Nature Publishing Group, 2016, pp. 644–48, doi:<a href=\"https://doi.org/10.1038/nature19774\">10.1038/nature19774</a>.","apa":"Letts, J. A., Fiedorczuk, K., &#38; Sazanov, L. A. (2016). The architecture of respiratory supercomplexes. <i>Nature</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/nature19774\">https://doi.org/10.1038/nature19774</a>","ista":"Letts JA, Fiedorczuk K, Sazanov LA. 2016. The architecture of respiratory supercomplexes. Nature. 537(7622), 644–648.","chicago":"Letts, James A, Karol Fiedorczuk, and Leonid A Sazanov. “The Architecture of Respiratory Supercomplexes.” <i>Nature</i>. Nature Publishing Group, 2016. <a href=\"https://doi.org/10.1038/nature19774\">https://doi.org/10.1038/nature19774</a>."},"issue":"7622","status":"public","publication":"Nature","language":[{"iso":"eng"}],"project":[{"_id":"2593EBD6-B435-11E9-9278-68D0E5697425","name":"Atomic-Resolution Structures of Mitochondrial Respiratory Chain Supercomplexes (FEBS)"}],"publist_id":"6102","department":[{"_id":"LeSa"}],"month":"09","year":"2016"}]