---
_id: '2223'
abstract:
- lang: eng
  text: Correct positioning of membrane proteins is an essential process in eukaryotic
    organisms. The plant hormone auxin is distributed through intercellular transport
    and triggers various cellular responses. Auxin transporters of the PIN-FORMED
    (PIN) family localize asymmetrically at the plasma membrane (PM) and mediate the
    directional transport of auxin between cells. A fungal toxin, brefeldin A (BFA),
    inhibits a subset of guanine nucleotide exchange factors for ADP-ribosylation
    factor small GTPases (ARF GEFs) including GNOM, which plays a major role in localization
    of PIN1 predominantly to the basal side of the PM. The Arabidopsis genome encodes
    19 ARF-related putative GTPases. However, ARF components involved in PIN1 localization
    have been genetically poorly defined. Using a fluorescence imaging-based forward
    genetic approach, we identified an Arabidopsis mutant, bfa-visualized exocytic
    trafficking defective1 (bex1), in which PM localization of PIN1-green fluorescent
    protein (GFP) as well as development is hypersensitive to BFA. We found that in
    bex1 a member of the ARF1 gene family, ARF1A1C, was mutated. ARF1A1C localizes
    to the trans-Golgi network/early endosome and Golgi apparatus, acts synergistically
    to BEN1/MIN7 ARF GEF and is important for PIN recycling to the PM. Consistent
    with the developmental importance of PIN proteins, functional interference with
    ARF1 resulted in an impaired auxin response gradient and various developmental
    defects including embryonic patterning defects and growth arrest. Our results
    show that ARF1A1C is essential for recycling of PIN auxin transporters and for
    various auxin-dependent developmental processes.
author:
- first_name: Hirokazu
  full_name: Tanaka, Hirokazu
  last_name: Tanaka
- first_name: Tomasz
  full_name: Nodzyński, Tomasz
  last_name: Nodzyński
- first_name: Saeko
  full_name: Kitakura, Saeko
  last_name: Kitakura
- first_name: Mugurel
  full_name: Feraru, Mugurel
  last_name: Feraru
- first_name: Michiko
  full_name: Sasabe, Michiko
  last_name: Sasabe
- first_name: Tomomi
  full_name: Ishikawa, Tomomi
  last_name: Ishikawa
- first_name: Jürgen
  full_name: Kleine Vehn, Jürgen
  last_name: Kleine Vehn
- first_name: Tatsuo
  full_name: Kakimoto, Tatsuo
  last_name: Kakimoto
- first_name: Jirí
  full_name: Friml, Jirí
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
citation:
  ama: Tanaka H, Nodzyński T, Kitakura S, et al. BEX1/ARF1A1C is required for BFA-sensitive
    recycling of PIN auxin transporters and auxin-mediated development in arabidopsis.
    <i>Plant and Cell Physiology</i>. 2014;55(4):737-749. doi:<a href="https://doi.org/10.1093/pcp/pct196">10.1093/pcp/pct196</a>
  apa: Tanaka, H., Nodzyński, T., Kitakura, S., Feraru, M., Sasabe, M., Ishikawa,
    T., … Friml, J. (2014). BEX1/ARF1A1C is required for BFA-sensitive recycling of
    PIN auxin transporters and auxin-mediated development in arabidopsis. <i>Plant
    and Cell Physiology</i>. Oxford University Press. <a href="https://doi.org/10.1093/pcp/pct196">https://doi.org/10.1093/pcp/pct196</a>
  chicago: Tanaka, Hirokazu, Tomasz Nodzyński, Saeko Kitakura, Mugurel Feraru, Michiko
    Sasabe, Tomomi Ishikawa, Jürgen Kleine Vehn, Tatsuo Kakimoto, and Jiří Friml.
    “BEX1/ARF1A1C Is Required for BFA-Sensitive Recycling of PIN Auxin Transporters
    and Auxin-Mediated Development in Arabidopsis.” <i>Plant and Cell Physiology</i>.
    Oxford University Press, 2014. <a href="https://doi.org/10.1093/pcp/pct196">https://doi.org/10.1093/pcp/pct196</a>.
  ieee: H. Tanaka <i>et al.</i>, “BEX1/ARF1A1C is required for BFA-sensitive recycling
    of PIN auxin transporters and auxin-mediated development in arabidopsis,” <i>Plant
    and Cell Physiology</i>, vol. 55, no. 4. Oxford University Press, pp. 737–749,
    2014.
  ista: Tanaka H, Nodzyński T, Kitakura S, Feraru M, Sasabe M, Ishikawa T, Kleine
    Vehn J, Kakimoto T, Friml J. 2014. BEX1/ARF1A1C is required for BFA-sensitive
    recycling of PIN auxin transporters and auxin-mediated development in arabidopsis.
    Plant and Cell Physiology. 55(4), 737–749.
  mla: Tanaka, Hirokazu, et al. “BEX1/ARF1A1C Is Required for BFA-Sensitive Recycling
    of PIN Auxin Transporters and Auxin-Mediated Development in Arabidopsis.” <i>Plant
    and Cell Physiology</i>, vol. 55, no. 4, Oxford University Press, 2014, pp. 737–49,
    doi:<a href="https://doi.org/10.1093/pcp/pct196">10.1093/pcp/pct196</a>.
  short: H. Tanaka, T. Nodzyński, S. Kitakura, M. Feraru, M. Sasabe, T. Ishikawa,
    J. Kleine Vehn, T. Kakimoto, J. Friml, Plant and Cell Physiology 55 (2014) 737–749.
date_created: 2018-12-11T11:56:25Z
date_published: 2014-04-01T00:00:00Z
date_updated: 2021-01-12T06:56:07Z
day: '01'
ddc:
- '570'
department:
- _id: JiFr
doi: 10.1093/pcp/pct196
ec_funded: 1
file:
- access_level: open_access
  checksum: b781a76b32ac35a520256453c3ba9433
  content_type: application/pdf
  creator: system
  date_created: 2018-12-12T10:14:25Z
  date_updated: 2020-07-14T12:45:34Z
  file_id: '5076'
  file_name: IST-2016-431-v1+1_Plant_Cell_Physiol-2014-Tanaka-737-49.pdf
  file_size: 2028111
  relation: main_file
file_date_updated: 2020-07-14T12:45:34Z
has_accepted_license: '1'
intvolume: '        55'
issue: '4'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: http://repository.ist.ac.at/id/eprint/431
month: '04'
oa: 1
oa_version: Published Version
page: 737 - 749
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '282300'
  name: Polarity and subcellular dynamics in plants
- _id: 256BDAB0-B435-11E9-9278-68D0E5697425
  name: Innovationsförderung in der Grenzregion Österreich – Tschechische Republik
    durch die Schaffung von Synergien im Bereich der Forschungsinfrastruktur
publication: Plant and Cell Physiology
publication_identifier:
  issn:
  - '00320781'
publication_status: published
publisher: Oxford University Press
publist_id: '4741'
pubrep_id: '431'
quality_controlled: '1'
scopus_import: 1
status: public
title: BEX1/ARF1A1C is required for BFA-sensitive recycling of PIN auxin transporters
  and auxin-mediated development in arabidopsis
tmp:
  image: /images/cc_by_nc.png
  legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
  short: CC BY-NC (4.0)
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 55
year: '2014'
...
---
_id: '2253'
abstract:
- lang: eng
  text: Plant growth is achieved predominantly by cellular elongation, which is thought
    to be controlled on several levels by apoplastic auxin. Auxin export into the
    apoplast is achieved by plasma membrane efflux catalysts of the PIN-FORMED (PIN)
    and ATP-binding cassette protein subfamily B/phosphor- glycoprotein (ABCB/PGP)
    classes; the latter were shown to depend on interaction with the FKBP42, TWISTED
    DWARF1 (TWD1). Here by using a transgenic approach in combination with phenotypical,
    biochemical and cell biological analyses we demonstrate the importance of a putative
    C-terminal in-plane membrane anchor of TWD1 in the regulation of ABCB-mediated
    auxin transport. In contrast with dwarfed twd1 loss-of-function alleles, TWD1
    gain-of-function lines that lack a putative in-plane membrane anchor (HA-TWD1-Ct)
    show hypermorphic plant architecture, characterized by enhanced stem length and
    leaf surface but reduced shoot branching. Greater hypocotyl length is the result
    of enhanced cell elongation that correlates with reduced polar auxin transport
    capacity for HA-TWD1-Ct. As a consequence, HA-TWD1-Ct displays higher hypocotyl
    auxin accumulation, which is shown to result in elevated auxin-induced cell elongation
    rates. Our data highlight the importance of C-terminal membrane anchoring for
    TWD1 action, which is required for specific regulation of ABCB-mediated auxin
    transport. These data support a model in which TWD1 controls lateral ABCB1-mediated
    export into the apoplast, which is required for auxin-mediated cell elongation.
article_processing_charge: No
article_type: original
author:
- first_name: Aurélien
  full_name: Bailly, Aurélien
  last_name: Bailly
- first_name: Bangjun
  full_name: Wang, Bangjun
  last_name: Wang
- first_name: Marta
  full_name: Zwiewka, Marta
  last_name: Zwiewka
- first_name: Stephan
  full_name: Pollmann, Stephan
  last_name: Pollmann
- first_name: Daniel
  full_name: Schenck, Daniel
  last_name: Schenck
- first_name: Hartwig
  full_name: Lüthen, Hartwig
  last_name: Lüthen
- first_name: Alexander
  full_name: Schulz, Alexander
  last_name: Schulz
- first_name: Jirí
  full_name: Friml, Jirí
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
- first_name: Markus
  full_name: Geisler, Markus
  last_name: Geisler
citation:
  ama: Bailly A, Wang B, Zwiewka M, et al. Expression of TWISTED DWARF1 lacking its
    in-plane membrane anchor leads to increased cell elongation and hypermorphic growth.
    <i>Plant Journal</i>. 2014;77(1):108-118. doi:<a href="https://doi.org/10.1111/tpj.12369">10.1111/tpj.12369</a>
  apa: Bailly, A., Wang, B., Zwiewka, M., Pollmann, S., Schenck, D., Lüthen, H., …
    Geisler, M. (2014). Expression of TWISTED DWARF1 lacking its in-plane membrane
    anchor leads to increased cell elongation and hypermorphic growth. <i>Plant Journal</i>.
    Wiley-Blackwell. <a href="https://doi.org/10.1111/tpj.12369">https://doi.org/10.1111/tpj.12369</a>
  chicago: Bailly, Aurélien, Bangjun Wang, Marta Zwiewka, Stephan Pollmann, Daniel
    Schenck, Hartwig Lüthen, Alexander Schulz, Jiří Friml, and Markus Geisler. “Expression
    of TWISTED DWARF1 Lacking Its In-Plane Membrane Anchor Leads to Increased Cell
    Elongation and Hypermorphic Growth.” <i>Plant Journal</i>. Wiley-Blackwell, 2014.
    <a href="https://doi.org/10.1111/tpj.12369">https://doi.org/10.1111/tpj.12369</a>.
  ieee: A. Bailly <i>et al.</i>, “Expression of TWISTED DWARF1 lacking its in-plane
    membrane anchor leads to increased cell elongation and hypermorphic growth,” <i>Plant
    Journal</i>, vol. 77, no. 1. Wiley-Blackwell, pp. 108–118, 2014.
  ista: Bailly A, Wang B, Zwiewka M, Pollmann S, Schenck D, Lüthen H, Schulz A, Friml
    J, Geisler M. 2014. Expression of TWISTED DWARF1 lacking its in-plane membrane
    anchor leads to increased cell elongation and hypermorphic growth. Plant Journal.
    77(1), 108–118.
  mla: Bailly, Aurélien, et al. “Expression of TWISTED DWARF1 Lacking Its In-Plane
    Membrane Anchor Leads to Increased Cell Elongation and Hypermorphic Growth.” <i>Plant
    Journal</i>, vol. 77, no. 1, Wiley-Blackwell, 2014, pp. 108–18, doi:<a href="https://doi.org/10.1111/tpj.12369">10.1111/tpj.12369</a>.
  short: A. Bailly, B. Wang, M. Zwiewka, S. Pollmann, D. Schenck, H. Lüthen, A. Schulz,
    J. Friml, M. Geisler, Plant Journal 77 (2014) 108–118.
date_created: 2018-12-11T11:56:35Z
date_published: 2014-01-01T00:00:00Z
date_updated: 2021-01-12T06:56:18Z
day: '01'
department:
- _id: JiFr
doi: 10.1111/tpj.12369
intvolume: '        77'
issue: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1111/tpj.12369
month: '01'
oa: 1
oa_version: Published Version
page: 108 - 118
project:
- _id: 256BDAB0-B435-11E9-9278-68D0E5697425
  name: Innovationsförderung in der Grenzregion Österreich – Tschechische Republik
    durch die Schaffung von Synergien im Bereich der Forschungsinfrastruktur
publication: Plant Journal
publication_identifier:
  issn:
  - '09607412'
publication_status: published
publisher: Wiley-Blackwell
publist_id: '4694'
quality_controlled: '1'
scopus_import: 1
status: public
title: Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to
  increased cell elongation and hypermorphic growth
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 77
year: '2014'
...