[{"_id":"13107","year":"2023","file_date_updated":"2023-06-07T08:41:49Z","date_created":"2023-06-01T09:05:24Z","page":"147","oa_version":"Published Version","month":"05","type":"dissertation","abstract":[{"text":"Within the human body, the brain exhibits the highest rate of energy consumption amongst all organs, with the majority of generated ATP being utilized to sustain neuronal activity. Therefore, the metabolism of the mature cerebral cortex is geared towards preserving metabolic homeostasis whilst generating significant amounts of energy. This requires a precise interplay between diverse metabolic pathways, spanning from a tissue-wide scale to the level of individual neurons. Disturbances to this delicate metabolic equilibrium, such as those resulting from maternal malnutrition\r\nor mutations affecting metabolic enzymes, often result in neuropathological variants of neurodevelopment. For instance, mutations in SLC7A5, a transporter of metabolically essential large neutral amino acids (LNAAs), have been associated with autism and microcephaly. However, despite recent progress in the field, the extent of metabolic restructuring that occurs within the developing brain and the corresponding alterations in nutrient demands during various critical periods remain largely unknown. To investigate this, we performed metabolomic profiling of the murine cerebral cortex to characterize the metabolic state of the forebrain at different developmental stages. We found that the developing cortex undergoes substantial metabolic reprogramming, with specific sets of metabolites displaying stage-specific changes. According to our observations, we determined a distinct temporal period in postnatal development during which the cortex displays heightened reliance on LNAAs. Hence, using a conditional knock-out mouse model, we deleted Slc7a5 in neural cells, allowing us to monitor the impact of a perturbed neuronal metabolic state across multiple developmental stages of corticogenesis. We found that manipulating the levels of essential LNAAs in cortical neurons in vivo affects one particular perinatal developmental period critical for cortical network refinement. Abnormally low intracellular LNAA levels result in cell-autonomous alterations in neuronal lipid metabolism, excitability, and survival during this particular time window. Although most of the effects of Slc7a5 deletion on neuronal physiology are transient, derailment of these processes during this brief but crucial window leads to long-term circuit dysfunction in mice. In conclusion, out data indicate that the cerebral cortex undergoes significant metabolic reorganization during development. This process involves the intricate integration of multiple metabolic pathways to ensure optimal neuronal function throughout different developmental stages. Our findings offer a paradigm for understanding how neurons synchronize the expression of nutrient-related genes with their activity to allow proper brain maturation. Further, our results demonstrate that disruptions in these precisely calibrated metabolic processes during critical periods of brain development may result in neuropathological outcomes in mice and in humans.","lang":"eng"}],"date_updated":"2024-02-07T08:03:33Z","related_material":{"record":[{"status":"public","id":"12802","relation":"part_of_dissertation"}]},"citation":{"apa":"Knaus, L. (2023). <i>The metabolism of the developing brain : How large neutral amino acids modulate perinatal neuronal excitability and survival</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/at:ista:13107\">https://doi.org/10.15479/at:ista:13107</a>","ista":"Knaus L. 2023. The metabolism of the developing brain : How large neutral amino acids modulate perinatal neuronal excitability and survival. Institute of Science and Technology Austria.","mla":"Knaus, Lisa. <i>The Metabolism of the Developing Brain : How Large Neutral Amino Acids Modulate Perinatal Neuronal Excitability and Survival</i>. Institute of Science and Technology Austria, 2023, doi:<a href=\"https://doi.org/10.15479/at:ista:13107\">10.15479/at:ista:13107</a>.","ama":"Knaus L. The metabolism of the developing brain : How large neutral amino acids modulate perinatal neuronal excitability and survival. 2023. doi:<a href=\"https://doi.org/10.15479/at:ista:13107\">10.15479/at:ista:13107</a>","short":"L. Knaus, The Metabolism of the Developing Brain : How Large Neutral Amino Acids Modulate Perinatal Neuronal Excitability and Survival, Institute of Science and Technology Austria, 2023.","chicago":"Knaus, Lisa. “The Metabolism of the Developing Brain : How Large Neutral Amino Acids Modulate Perinatal Neuronal Excitability and Survival.” Institute of Science and Technology Austria, 2023. <a href=\"https://doi.org/10.15479/at:ista:13107\">https://doi.org/10.15479/at:ista:13107</a>.","ieee":"L. Knaus, “The metabolism of the developing brain : How large neutral amino acids modulate perinatal neuronal excitability and survival,” Institute of Science and Technology Austria, 2023."},"alternative_title":["ISTA Thesis"],"status":"public","ddc":["570"],"date_published":"2023-05-31T00:00:00Z","supervisor":[{"full_name":"Novarino, Gaia","orcid":"0000-0002-7673-7178","id":"3E57A680-F248-11E8-B48F-1D18A9856A87","first_name":"Gaia","last_name":"Novarino"}],"acknowledged_ssus":[{"_id":"PreCl"},{"_id":"Bio"},{"_id":"EM-Fac"}],"oa":1,"publication_status":"published","has_accepted_license":"1","article_processing_charge":"No","ec_funded":1,"user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","publisher":"Institute of Science and Technology Austria","department":[{"_id":"GradSch"},{"_id":"GaNo"}],"title":"The metabolism of the developing brain : How large neutral amino acids modulate perinatal neuronal excitability and survival","degree_awarded":"PhD","author":[{"last_name":"Knaus","first_name":"Lisa","id":"3B2ABCF4-F248-11E8-B48F-1D18A9856A87","full_name":"Knaus, Lisa"}],"file":[{"access_level":"closed","date_created":"2023-06-01T13:48:41Z","checksum":"4b69a4ac0bbf4163d59c0b58dcb4f2c3","file_id":"13112","date_updated":"2023-06-01T13:48:41Z","creator":"lknaus","file_size":12991551,"relation":"source_file","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","file_name":"Thesis_Lisa Knaus_approved_final.docx"},{"access_level":"open_access","date_created":"2023-06-02T09:47:29Z","checksum":"6903d152aa01181d87a696085af31c83","date_updated":"2023-06-07T08:41:49Z","file_id":"13114","creator":"lknaus","content_type":"application/pdf","relation":"main_file","file_size":9309015,"file_name":"Thesis_Lisa Knaus_approved_final_pdfa2b.pdf"}],"day":"31","language":[{"iso":"eng"}],"project":[{"call_identifier":"H2020","_id":"25444568-B435-11E9-9278-68D0E5697425","name":"Probing the Reversibility of Autism Spectrum Disorders by Employing in vivo and in vitro Models","grant_number":"715508"},{"name":"Molecular Drug Targets","_id":"2548AE96-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","grant_number":"W1232-B24"}],"doi":"10.15479/at:ista:13107","publication_identifier":{"issn":["2663 - 337X"]}},{"publisher":"Institute of Science and Technology Austria","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"JoDa"},{"_id":"SaSi"},{"_id":"GaNo"},{"_id":"PeJo"},{"_id":"Bio"},{"_id":"RySh"}],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode","image":"/images/cc_by_nc_sa.png","name":"Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)","short":"CC BY-NC-SA (4.0)"},"article_processing_charge":"No","ec_funded":1,"author":[{"first_name":"Johann G","last_name":"Danzl","full_name":"Danzl, Johann G","orcid":"0000-0001-8559-3973","id":"42EFD3B6-F248-11E8-B48F-1D18A9856A87"}],"file":[{"date_created":"2023-08-04T13:19:47Z","access_level":"open_access","date_updated":"2023-08-04T13:19:47Z","file_id":"13961","checksum":"6f18ce9b89b47ce5abeb379869ff5c49","content_type":"text/plain","relation":"main_file","file_size":541,"creator":"jdanzl","success":1,"file_name":"Readme_Michalska_2023.txt"},{"checksum":"2098e8c5285c5e86cb69075e1b5dcf39","date_updated":"2023-08-03T11:29:29Z","file_id":"13482","access_level":"open_access","date_created":"2023-08-03T11:29:29Z","success":1,"file_name":"Fig1_b_top-left.tif","creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":64582744},{"checksum":"9affab3dacf847096cc8e8d5ff99467e","date_updated":"2023-08-03T11:29:28Z","file_id":"13483","access_level":"open_access","date_created":"2023-08-03T11:29:28Z","success":1,"file_name":"Fig1_b_top-right.tif","creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":1442113},{"creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":40078276,"success":1,"file_name":"Fig1_c_1.tif","access_level":"open_access","date_created":"2023-08-03T11:29:29Z","checksum":"d7fec0296123a17f6bcdbfee17411424","date_updated":"2023-08-03T11:29:29Z","file_id":"13484"},{"date_created":"2023-08-03T11:29:29Z","access_level":"open_access","date_updated":"2023-08-03T11:29:29Z","file_id":"13485","checksum":"5195a180c4f26a7adc994bad0b3c53c7","content_type":"image/tiff","relation":"main_file","file_size":7984315,"creator":"jdanzl","success":1,"file_name":"Fig1_c_2.tif"},{"creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":8693320,"success":1,"file_name":"Fig1_c_3.tif","access_level":"open_access","date_created":"2023-08-03T11:29:29Z","checksum":"df1105d8f41ebd5373cacc597ffa91f3","date_updated":"2023-08-03T11:29:29Z","file_id":"13486"},{"date_created":"2023-08-03T11:29:30Z","access_level":"open_access","file_id":"13487","date_updated":"2023-08-03T11:29:30Z","checksum":"00edbb0c9f42032c830d2291ec55c4c0","file_size":6003063,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl","file_name":"Fig1_c_4.tif","success":1},{"creator":"jdanzl","file_size":24003063,"content_type":"image/tiff","relation":"main_file","file_name":"Fig1_d.tif","success":1,"access_level":"open_access","date_created":"2023-08-03T11:29:30Z","checksum":"a58399e98ec4b6506a5dae13e387f391","file_id":"13488","date_updated":"2023-08-03T11:29:30Z"},{"success":1,"file_name":"Fig1_e_zoom.tif","content_type":"image/tiff","relation":"main_file","file_size":18063,"creator":"jdanzl","date_updated":"2023-08-03T11:29:30Z","file_id":"13489","checksum":"699df4bc104df14607359678963b3d2d","date_created":"2023-08-03T11:29:30Z","access_level":"open_access"},{"access_level":"open_access","date_created":"2023-08-03T11:29:30Z","checksum":"ea7b1278da490d221fc7698fd6bc39a4","date_updated":"2023-08-03T11:29:30Z","file_id":"13490","creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":186813,"success":1,"file_name":"Fig1_e.tif"},{"success":1,"file_name":"Fig1_f.xlsx","relation":"main_file","content_type":"application/vnd.openxmlformats-officedocument.spreadsheetml.sheet","file_size":17494,"creator":"jdanzl","date_updated":"2023-08-03T11:29:30Z","file_id":"13491","checksum":"37b05ef3a36b9f6bf4ec46199230874a","date_created":"2023-08-03T11:29:30Z","access_level":"open_access"},{"checksum":"872eb3dc1a94afb26947a365bfae581a","date_updated":"2023-08-03T11:40:43Z","file_id":"13492","access_level":"open_access","date_created":"2023-08-03T11:40:43Z","success":1,"file_name":"Fig2_a_1.tif","creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":816786862},{"creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":8654692,"success":1,"file_name":"Fig2_a_2.tif","access_level":"open_access","date_created":"2023-08-03T11:40:13Z","checksum":"cab6f72d7d546a81c105c229747949ef","date_updated":"2023-08-03T11:40:13Z","file_id":"13493"},{"file_id":"13494","date_updated":"2023-08-03T11:40:14Z","checksum":"c85bc3ffe7f6bdf16a81290761ed6253","date_created":"2023-08-03T11:40:14Z","access_level":"open_access","file_name":"Fig2_a_3.tif","success":1,"file_size":8654692,"content_type":"image/tiff","relation":"main_file","creator":"jdanzl"},{"file_id":"13495","date_updated":"2023-08-03T11:40:24Z","checksum":"5a3fd5484e566c34e210a4acb2b97791","date_created":"2023-08-03T11:40:24Z","access_level":"open_access","file_name":"Fig2_a_4.tif","success":1,"file_size":272262438,"content_type":"image/tiff","relation":"main_file","creator":"jdanzl"},{"date_created":"2023-08-03T11:40:37Z","access_level":"open_access","file_id":"13496","date_updated":"2023-08-03T11:40:37Z","checksum":"ae5cbacf56e3fdf934a6d6fd489f5fe0","file_size":272262438,"content_type":"image/tiff","relation":"main_file","creator":"jdanzl","file_name":"Fig2_a_5.tif","success":1},{"creator":"jdanzl","file_size":204301228,"content_type":"image/tiff","relation":"main_file","file_name":"Fig2_a_6.tif","success":1,"access_level":"open_access","date_created":"2023-08-03T11:40:44Z","checksum":"e6da436b83bbe3a34138b52e5de49d69","file_id":"13497","date_updated":"2023-08-03T11:40:44Z"},{"checksum":"855f0a0bcf5b3d7cc7d50561f2c8f625","date_updated":"2023-08-03T11:40:49Z","file_id":"13498","access_level":"open_access","date_created":"2023-08-03T11:40:49Z","success":1,"file_name":"Fig2_a_7-8.tif","creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":68094350},{"date_created":"2023-08-03T11:40:48Z","access_level":"open_access","file_id":"13499","date_updated":"2023-08-03T11:40:48Z","checksum":"ff709f9f8ebaa8cf50a4b2fca5ce9813","file_size":156,"relation":"main_file","content_type":"application/octet-stream","creator":"jdanzl","file_name":"Fig2_a_roi.roi","success":1},{"date_created":"2023-08-03T11:41:37Z","access_level":"open_access","file_id":"13500","date_updated":"2023-08-03T11:41:37Z","checksum":"ce9b774ff5163ebfa4e3fb162f4cfffe","file_size":1036927887,"content_type":"image/tiff","relation":"main_file","creator":"jdanzl","file_name":"Fig2_c-i_1_n2v.tif","success":1},{"success":1,"file_name":"Fig2_c-i_1.tif","content_type":"image/tiff","relation":"main_file","file_size":518519275,"creator":"jdanzl","date_updated":"2023-08-03T11:41:25Z","file_id":"13501","checksum":"21f1bb8d2a00c88bdd6febe6285acc2a","date_created":"2023-08-03T11:41:25Z","access_level":"open_access"},{"file_size":1444202302,"content_type":"image/tiff","relation":"main_file","creator":"jdanzl","file_name":"Fig2_c-i_3_n2v.tif","success":1,"date_created":"2023-08-03T11:42:48Z","access_level":"open_access","file_id":"13502","date_updated":"2023-08-03T11:42:48Z","checksum":"9af5b66b74c2ff4b2b99b0d94f2864e6"},{"date_created":"2023-08-03T11:42:57Z","access_level":"open_access","date_updated":"2023-08-03T11:42:57Z","file_id":"13503","checksum":"c83261e88821747b301197314d92f2b2","relation":"main_file","content_type":"image/tiff","file_size":1075338708,"creator":"jdanzl","success":1,"file_name":"Fig2_c-i_3.tif"},{"date_created":"2023-08-03T11:44:05Z","access_level":"open_access","date_updated":"2023-08-03T11:44:05Z","file_id":"13504","checksum":"5d1278ba4b7e085c0c186ea49fce535b","content_type":"image/tiff","relation":"main_file","file_size":851145493,"creator":"jdanzl","success":1,"file_name":"Fig2_c-i_2_n2v.tif"},{"file_size":432099834,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl","file_name":"Fig2_c-i_2.tif","success":1,"date_created":"2023-08-03T11:43:47Z","access_level":"open_access","file_id":"13505","date_updated":"2023-08-03T11:43:47Z","checksum":"5aaf1eba65582cf437ff3ce753eba4d7"},{"content_type":"image/tiff","relation":"main_file","file_size":1036927887,"creator":"jdanzl","success":1,"file_name":"Fig2_h_n2v.tif","date_created":"2023-08-03T11:44:41Z","access_level":"open_access","date_updated":"2023-08-03T11:44:41Z","file_id":"13506","checksum":"ce9b774ff5163ebfa4e3fb162f4cfffe"},{"checksum":"21f1bb8d2a00c88bdd6febe6285acc2a","file_id":"13507","date_updated":"2023-08-03T11:44:36Z","access_level":"open_access","date_created":"2023-08-03T11:44:36Z","file_name":"Fig2_h.tif","success":1,"creator":"jdanzl","file_size":518519275,"relation":"main_file","content_type":"image/tiff"},{"date_updated":"2023-08-03T11:44:40Z","file_id":"13508","checksum":"b37f25a90ef42fa4358649fdd6b41f35","date_created":"2023-08-03T11:44:40Z","access_level":"open_access","success":1,"file_name":"Fig2_j_1.tif","relation":"main_file","content_type":"image/tiff","file_size":19287397,"creator":"jdanzl"},{"date_updated":"2023-08-03T11:44:41Z","file_id":"13509","checksum":"50f0969c6c2c1209ff776bec4861030a","date_created":"2023-08-03T11:44:41Z","access_level":"open_access","success":1,"file_name":"Fig2_j_2.tif","content_type":"image/tiff","relation":"main_file","file_size":617552,"creator":"jdanzl"},{"date_created":"2023-08-03T11:44:41Z","access_level":"open_access","date_updated":"2023-08-03T11:44:41Z","file_id":"13510","checksum":"2a8cc93471378ac4c3b640e4318d89eb","content_type":"image/tiff","relation":"main_file","file_size":19287392,"creator":"jdanzl","success":1,"file_name":"Fig2_j_3.tif"},{"content_type":"image/tiff","relation":"main_file","file_size":617552,"creator":"jdanzl","success":1,"file_name":"Fig2_j_4.tif","date_created":"2023-08-03T11:44:42Z","access_level":"open_access","date_updated":"2023-08-03T11:44:42Z","file_id":"13511","checksum":"b90965d20133fea73c99050c32eb7695"},{"access_level":"open_access","date_created":"2023-08-03T11:44:43Z","checksum":"568a1e0efaa62527b51e66a7aabfd05c","file_id":"13512","date_updated":"2023-08-03T11:44:43Z","creator":"jdanzl","file_size":19287339,"relation":"main_file","content_type":"image/tiff","file_name":"Fig2_j_coCATS_N2V.tif","success":1},{"file_id":"13513","date_updated":"2023-08-03T11:44:44Z","checksum":"c70447cebe073dd78e0ac34ecc36ad85","date_created":"2023-08-03T11:44:44Z","access_level":"open_access","file_name":"Fig2_j_roi.roi","success":1,"file_size":148,"relation":"main_file","content_type":"application/octet-stream","creator":"jdanzl"},{"file_id":"13514","date_updated":"2023-08-03T11:44:52Z","checksum":"505252cc4b443b76c2e41ecf5879a542","date_created":"2023-08-03T11:44:52Z","access_level":"open_access","file_name":"Fig3_a_n2v.tif","success":1,"file_size":316842690,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl"},{"date_created":"2023-08-03T11:45:01Z","access_level":"open_access","date_updated":"2023-08-03T11:45:01Z","file_id":"13515","checksum":"35463183611e8ee4fba2025772c40eb2","relation":"main_file","content_type":"image/tiff","file_size":172839057,"creator":"jdanzl","success":1,"file_name":"Fig3_a.tif"},{"file_id":"13516","date_updated":"2023-08-03T11:45:05Z","checksum":"e0469102a87332b46904b4ba35b8fb2f","date_created":"2023-08-03T11:45:05Z","access_level":"open_access","file_name":"Fig3_g_n2v.tif","success":1,"file_size":309407894,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl"},{"creator":"jdanzl","file_size":11327792,"content_type":"image/tiff","relation":"main_file","file_name":"Fig4_a_MIP.tif","success":1,"access_level":"open_access","date_created":"2023-08-03T11:51:15Z","checksum":"854c8fe02acd920f8e8f6a3173458e86","file_id":"13517","date_updated":"2023-08-03T11:51:15Z"},{"access_level":"open_access","date_created":"2023-08-03T11:51:18Z","checksum":"ab35811ffbe3f40733d0e054d9d6c4e6","date_updated":"2023-08-03T11:51:18Z","file_id":"13518","creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":106696700,"success":1,"file_name":"Fig4_c_dghilus_bouton2_n2v.tif"},{"access_level":"open_access","date_created":"2023-08-03T11:51:17Z","checksum":"e3b6410d68e42004ef3857967707aeb3","date_updated":"2023-08-03T11:51:17Z","file_id":"13519","creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":53367287,"success":1,"file_name":"Fig4_c_dghilus_bouton2.tif"},{"creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":103816699,"success":1,"file_name":"Fig4_c_dghilus_bouton5_n2v.tif","access_level":"open_access","date_created":"2023-08-03T11:51:20Z","checksum":"9ef0e3f3ec6f0b7b2666e3a0d7e6f355","date_updated":"2023-08-03T11:51:20Z","file_id":"13520"},{"checksum":"d5d846a4edaf0168ca6cc53d8cc39dde","date_updated":"2023-08-03T11:51:21Z","file_id":"13521","access_level":"open_access","date_created":"2023-08-03T11:51:21Z","success":1,"file_name":"Fig4_c_dghilus_bouton5.tif","creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":51927287},{"success":1,"file_name":"Fig4_c_stratum_bouton11_n2v.tif","creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":76391971,"checksum":"690a87182441c9742da2550189641e5a","date_updated":"2023-08-03T11:51:24Z","file_id":"13522","access_level":"open_access","date_created":"2023-08-03T11:51:24Z"},{"date_created":"2023-08-03T11:51:23Z","access_level":"open_access","file_id":"13523","date_updated":"2023-08-03T11:51:23Z","checksum":"4dc575424729c5ba181bd9a3962d4781","file_size":38217920,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl","file_name":"Fig4_c_stratum_bouton11.tif","success":1},{"date_created":"2023-08-03T11:51:29Z","access_level":"open_access","file_id":"13524","date_updated":"2023-08-03T11:51:29Z","checksum":"9fdd51636928f45e8e534cc5a37a48ff","file_size":154149020,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl","file_name":"Fig4_c_stratum_bouton13_n2v.tif","success":1},{"file_size":77099447,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl","file_name":"Fig4_c_stratum_bouton13.tif","success":1,"date_created":"2023-08-03T11:51:28Z","access_level":"open_access","file_id":"13525","date_updated":"2023-08-03T11:51:28Z","checksum":"643169464252d67753ac6f8493668f34"},{"file_name":"Fig4_d.tif","success":1,"creator":"jdanzl","file_size":106696700,"content_type":"image/tiff","relation":"main_file","checksum":"ab35811ffbe3f40733d0e054d9d6c4e6","file_id":"13526","date_updated":"2023-08-03T11:51:32Z","access_level":"open_access","date_created":"2023-08-03T11:51:32Z"},{"relation":"main_file","content_type":"image/tiff","file_size":154149020,"creator":"jdanzl","success":1,"file_name":"Fig4_e.tif","date_created":"2023-08-03T11:51:35Z","access_level":"open_access","date_updated":"2023-08-03T11:51:35Z","file_id":"13527","checksum":"9fdd51636928f45e8e534cc5a37a48ff"},{"success":1,"file_name":"Fig4_f_1_top.tif","creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":5986760,"checksum":"5380d0a98e97d7d8462b38bd0a96644c","date_updated":"2023-08-03T11:51:32Z","file_id":"13528","access_level":"open_access","date_created":"2023-08-03T11:51:32Z"},{"date_updated":"2023-08-03T11:51:33Z","file_id":"13529","checksum":"7d1309e806eb31588ebafcf40388e13f","date_created":"2023-08-03T11:51:33Z","access_level":"open_access","success":1,"file_name":"Fig4_f_2_top.tif","content_type":"image/tiff","relation":"main_file","file_size":6001178,"creator":"jdanzl"},{"date_created":"2023-08-03T11:51:33Z","access_level":"open_access","file_id":"13530","date_updated":"2023-08-03T11:51:33Z","checksum":"a71c80207655d508cbc7626bc144d7a1","file_size":6023318,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl","file_name":"Fig4_f_3_top.tif","success":1},{"date_created":"2023-08-03T11:51:34Z","access_level":"open_access","file_id":"13531","date_updated":"2023-08-03T11:51:34Z","checksum":"5d76b2ad7d7acbb1afa27cf52194b1cb","file_size":6038186,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl","file_name":"Fig4_f_4_top.tif","success":1},{"checksum":"9cee6b3387bd880843f2c6cdfa0d4a8d","file_id":"13532","date_updated":"2023-08-03T11:51:34Z","access_level":"open_access","date_created":"2023-08-03T11:51:34Z","file_name":"Fig4_f_5_top.tif","success":1,"creator":"jdanzl","file_size":2324158,"content_type":"image/tiff","relation":"main_file"},{"checksum":"a48fc9e1bb2710cf28466ae6d2a20c19","file_id":"13533","date_updated":"2023-08-03T11:51:34Z","access_level":"open_access","date_created":"2023-08-03T11:51:34Z","file_name":"Fig4_f_1_bottom.png","success":1,"creator":"jdanzl","file_size":1150264,"content_type":"image/png","relation":"main_file"},{"date_updated":"2023-08-03T11:51:34Z","file_id":"13534","checksum":"ff47ca30f814e17561974b73b0913a1a","date_created":"2023-08-03T11:51:34Z","access_level":"open_access","success":1,"file_name":"Fig4_f_2_bottom.png","content_type":"image/png","relation":"main_file","file_size":1157424,"creator":"jdanzl"},{"checksum":"2ea3fb8d79cee8ae9de7ca31d003910f","date_updated":"2023-08-03T11:51:34Z","file_id":"13535","access_level":"open_access","date_created":"2023-08-03T11:51:34Z","success":1,"file_name":"Fig4_f_3_bottom.png","creator":"jdanzl","relation":"main_file","content_type":"image/png","file_size":1152715},{"date_created":"2023-08-03T11:51:34Z","access_level":"open_access","date_updated":"2023-08-03T11:51:34Z","file_id":"13536","checksum":"d25e5ecf2f0c3be7c20a7e25a7a334b8","relation":"main_file","content_type":"image/png","file_size":1105240,"creator":"jdanzl","success":1,"file_name":"Fig4_f_4_bottom.png"},{"success":1,"file_name":"Fig4_f_5_bottom.png","creator":"jdanzl","content_type":"image/png","relation":"main_file","file_size":1228157,"checksum":"76535f569a57b967e378df89b7d2982a","date_updated":"2023-08-03T11:51:35Z","file_id":"13537","access_level":"open_access","date_created":"2023-08-03T11:51:35Z"},{"success":1,"file_name":"Fig5_a_1.tif","creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":91181184,"checksum":"d46fefe73daa3ec08b847631e9de6828","date_updated":"2023-08-03T11:53:52Z","file_id":"13538","access_level":"open_access","date_created":"2023-08-03T11:53:52Z"},{"date_created":"2023-08-03T11:53:54Z","access_level":"open_access","file_id":"13539","date_updated":"2023-08-03T11:53:54Z","checksum":"354c0ba876a33672eca37cd61dac7073","file_size":176024124,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl","file_name":"Fig5_a_2.tif","success":1},{"file_name":"Fig5_a_3.tif","success":1,"creator":"jdanzl","file_size":15812494,"content_type":"image/tiff","relation":"main_file","checksum":"d07925040ef0c3c51edbdca8cf3ec1fa","file_id":"13540","date_updated":"2023-08-03T11:53:53Z","access_level":"open_access","date_created":"2023-08-03T11:53:53Z"},{"success":1,"file_name":"Fig5_a_4.tif","content_type":"image/tiff","relation":"main_file","file_size":11117689,"creator":"jdanzl","date_updated":"2023-08-03T11:53:53Z","file_id":"13541","checksum":"7c34b19a1764e2f501f11dcabd442ce2","date_created":"2023-08-03T11:53:53Z","access_level":"open_access"},{"file_size":111665,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl","file_name":"Fig5_a_5.tif","success":1,"date_created":"2023-08-03T11:53:53Z","access_level":"open_access","file_id":"13542","date_updated":"2023-08-03T11:53:53Z","checksum":"c49af4b96532e00b3a51574a2a94decb"},{"success":1,"file_name":"Fig5_b_1.tif","relation":"main_file","content_type":"image/tiff","file_size":16676449,"creator":"jdanzl","date_updated":"2023-08-03T11:53:54Z","file_id":"13543","checksum":"1b42eadcdd71f2dc00d82784673aa577","date_created":"2023-08-03T11:53:54Z","access_level":"open_access"},{"file_name":"Fig5_b_2-4.tif","success":1,"creator":"jdanzl","file_size":427651,"relation":"main_file","content_type":"image/tiff","checksum":"0d27266bf216f71fcb5496731ce76dd5","file_id":"13544","date_updated":"2023-08-03T11:53:54Z","access_level":"open_access","date_created":"2023-08-03T11:53:54Z"},{"creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":11117705,"success":1,"file_name":"Fig5_c_1-3.tif","access_level":"open_access","date_created":"2023-08-03T11:53:55Z","checksum":"c4232930068a0a8f7c8a70ce0ea48dc2","date_updated":"2023-08-03T11:53:55Z","file_id":"13545"},{"access_level":"open_access","date_created":"2023-08-03T11:53:55Z","checksum":"c9caad749be0ef04ef8013cfdb69f8fc","file_id":"13546","date_updated":"2023-08-03T11:53:55Z","creator":"jdanzl","file_size":254153,"content_type":"image/tiff","relation":"main_file","file_name":"Fig5_c_2-4.tif","success":1},{"date_created":"2023-08-03T11:54:14Z","access_level":"open_access","date_updated":"2023-08-03T11:54:14Z","file_id":"13547","checksum":"5c6d36b96bde1e090cc428b269aba6ec","content_type":"image/tiff","relation":"main_file","file_size":737290746,"creator":"jdanzl","success":1,"file_name":"Fig5_e_1.tif"},{"relation":"main_file","content_type":"image/tiff","file_size":31366288,"creator":"jdanzl","success":1,"file_name":"Fig5_e_2.tif","date_created":"2023-08-03T11:53:57Z","access_level":"open_access","date_updated":"2023-08-03T11:53:57Z","file_id":"13548","checksum":"f97632984b6a73e06cc0c8c947cdcc71"},{"checksum":"8c471eeaa59008eed9e91afe5e1f1698","file_id":"13549","date_updated":"2023-08-03T11:53:57Z","access_level":"open_access","date_created":"2023-08-03T11:53:57Z","file_name":"Fig5_e_3.tif","success":1,"creator":"jdanzl","file_size":1216304,"content_type":"image/tiff","relation":"main_file"},{"content_type":"image/tiff","relation":"main_file","file_size":35669193,"creator":"jdanzl","success":1,"file_name":"Fig5_g.tif","date_created":"2023-08-03T11:53:59Z","access_level":"open_access","date_updated":"2023-08-03T11:53:59Z","file_id":"13550","checksum":"31c10c3a1d874b5028e35e2dd8800a68"},{"success":1,"file_name":"Fig5_h.tif","creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":7492349,"checksum":"0e9fe7b7ee06a630837e9d3c41b80e01","date_updated":"2023-08-03T11:53:59Z","file_id":"13551","access_level":"open_access","date_created":"2023-08-03T11:53:59Z"},{"file_size":121446390,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl","file_name":"Fig6_a_1.tif","success":1,"date_created":"2023-08-03T11:59:18Z","access_level":"open_access","file_id":"13552","date_updated":"2023-08-03T11:59:18Z","checksum":"d25b3d04bb8364089cafdf8deb6846b6"},{"file_id":"13553","date_updated":"2023-08-03T11:59:22Z","checksum":"4361e10db6ec48c2d4579ac089ff3ca3","date_created":"2023-08-03T11:59:22Z","access_level":"open_access","file_name":"Fig6_a_2.tif","success":1,"file_size":264412158,"content_type":"image/tiff","relation":"main_file","creator":"jdanzl"},{"date_created":"2023-08-03T11:59:21Z","access_level":"open_access","date_updated":"2023-08-03T11:59:21Z","file_id":"13554","checksum":"fe3a00ae75fb3388438e53ced92a9498","content_type":"image/tiff","relation":"main_file","file_size":57399051,"creator":"jdanzl","success":1,"file_name":"Fig6_a_3.tif"},{"file_size":6003163,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl","file_name":"Fig6_b_left.tif","success":1,"date_created":"2023-08-03T11:59:21Z","access_level":"open_access","file_id":"13555","date_updated":"2023-08-03T11:59:21Z","checksum":"4372c13d6dd2274aa749799b8e96bf06"},{"date_updated":"2023-08-03T11:59:21Z","file_id":"13556","checksum":"f7ae4e50f2f4d414c598bddd691b722a","date_created":"2023-08-03T11:59:21Z","access_level":"open_access","success":1,"file_name":"Fig6_b_right.tif","content_type":"image/tiff","relation":"main_file","file_size":511265,"creator":"jdanzl"},{"file_name":"Fig6_c_n2v.tif","success":1,"creator":"jdanzl","file_size":467125933,"content_type":"image/tiff","relation":"main_file","checksum":"cde658a9ca666bdef1b29613ab72092d","file_id":"13557","date_updated":"2023-08-03T11:59:39Z","access_level":"open_access","date_created":"2023-08-03T11:59:39Z"},{"date_created":"2023-08-03T11:59:36Z","access_level":"open_access","date_updated":"2023-08-03T11:59:36Z","file_id":"13558","checksum":"57e73c1fedcee4bd8ab62518baa0e0e5","relation":"main_file","content_type":"image/tiff","file_size":322251440,"creator":"jdanzl","success":1,"file_name":"Fig6_c.tif"},{"file_size":37641738,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl","file_name":"Fig6_d_1.tif","success":1,"date_created":"2023-08-03T11:59:38Z","access_level":"open_access","file_id":"13559","date_updated":"2023-08-03T11:59:38Z","checksum":"53175073b83ab569241ae64ef7f180b3"},{"date_created":"2023-08-03T11:59:40Z","access_level":"open_access","date_updated":"2023-08-03T11:59:40Z","file_id":"13560","checksum":"af97423e1077af85465a5e39fa25d1e4","content_type":"image/tiff","relation":"main_file","file_size":70596929,"creator":"jdanzl","success":1,"file_name":"Fig6_d_2.tif"},{"creator":"jdanzl","file_size":15303146,"relation":"main_file","content_type":"image/tiff","file_name":"Fig6_d_3.tif","success":1,"access_level":"open_access","date_created":"2023-08-03T11:59:42Z","checksum":"f1fd7a9fa2a12a930fe35bdf53ba3ff7","file_id":"13561","date_updated":"2023-08-03T11:59:42Z"},{"date_created":"2023-08-03T11:59:41Z","access_level":"open_access","file_id":"13562","date_updated":"2023-08-03T11:59:41Z","checksum":"95655df5cdb05f09912298de9a3711b4","file_size":893051,"content_type":"image/tiff","relation":"main_file","creator":"jdanzl","file_name":"Fig6_d_4.tif","success":1},{"date_created":"2023-08-03T11:59:58Z","access_level":"open_access","file_id":"13563","date_updated":"2023-08-03T11:59:58Z","checksum":"4d722b4e09ce617252d284f78ea2ee30","file_size":381232200,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl","file_name":"Fig6_e.tif","success":1},{"file_name":"Fig6_f.tif","success":1,"file_size":345804470,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl","file_id":"13564","date_updated":"2023-08-03T11:59:58Z","checksum":"d1eda9599f422a614404dd112f0c3185","date_created":"2023-08-03T11:59:58Z","access_level":"open_access"},{"access_level":"open_access","date_created":"2023-08-03T12:00:08Z","checksum":"1cd202570f964b78e1f55c987d5d5de1","date_updated":"2023-08-03T12:00:08Z","file_id":"13565","creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":346119927,"success":1,"file_name":"Fig6_g.tif"},{"content_type":"image/tiff","relation":"main_file","file_size":7064977,"creator":"jdanzl","success":1,"file_name":"Fig6_h.tif","date_created":"2023-08-03T12:00:02Z","access_level":"open_access","date_updated":"2023-08-03T12:00:02Z","file_id":"13566","checksum":"78cbf1906ff8746ed524c62f8f580f52"},{"file_name":"Fig6_i.tif","success":1,"creator":"jdanzl","file_size":7110129,"content_type":"image/tiff","relation":"main_file","checksum":"21635ce59f1ae5a334aaad3f4c0b171c","file_id":"13567","date_updated":"2023-08-03T12:00:03Z","access_level":"open_access","date_created":"2023-08-03T12:00:03Z"},{"creator":"jdanzl","file_size":110297540,"relation":"main_file","content_type":"image/tiff","file_name":"Fig6_j.tif","success":1,"access_level":"open_access","date_created":"2023-08-03T12:00:07Z","checksum":"704962856f2a024ce1f321a1785556e3","file_id":"13568","date_updated":"2023-08-03T12:00:07Z"},{"creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":11117689,"success":1,"file_name":"Fig6_k.tif","access_level":"open_access","date_created":"2023-08-03T12:00:07Z","checksum":"4b74ba5ae1e2bb2201bf51c74d733268","date_updated":"2023-08-03T12:00:07Z","file_id":"13569"},{"success":1,"file_name":"Extdata_01_a_1.tif","relation":"main_file","content_type":"image/tiff","file_size":1287107,"creator":"jdanzl","date_updated":"2023-08-03T12:05:41Z","file_id":"13570","checksum":"a8efa3007df4e9e0f80c7a2bbc451178","date_created":"2023-08-03T12:05:41Z","access_level":"open_access"},{"success":1,"file_name":"Extdata_01_a_2.tif","creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":58307,"checksum":"7f24b6c84ce96a67347eb5ef4c43fac1","date_updated":"2023-08-03T12:05:41Z","file_id":"13571","access_level":"open_access","date_created":"2023-08-03T12:05:41Z"},{"file_size":1283209,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl","file_name":"Extdata_01_a_3.tif","success":1,"date_created":"2023-08-03T12:05:41Z","access_level":"open_access","file_id":"13572","date_updated":"2023-08-03T12:05:41Z","checksum":"fa7ae4eefc11b9b60c4ab80f6e839b73"},{"access_level":"open_access","date_created":"2023-08-03T12:05:41Z","checksum":"cec9a5eb87f1b56e64626a00c6d0e53d","file_id":"13573","date_updated":"2023-08-03T12:05:41Z","creator":"jdanzl","file_size":54409,"relation":"main_file","content_type":"image/tiff","file_name":"Extdata_01_a_4.tif","success":1},{"success":1,"file_name":"Extdata_01_c_1.png","creator":"jdanzl","content_type":"image/png","relation":"main_file","file_size":2431,"checksum":"450e4786d66d5059028420153908a4dc","date_updated":"2023-08-03T12:05:41Z","file_id":"13574","access_level":"open_access","date_created":"2023-08-03T12:05:41Z"},{"date_created":"2023-08-03T12:05:41Z","access_level":"open_access","date_updated":"2023-08-03T12:05:41Z","file_id":"13575","checksum":"feb758609b399cf644dd3ae7cd75fb96","content_type":"image/png","relation":"main_file","file_size":612,"creator":"jdanzl","success":1,"file_name":"Extdata_01_c_2.png"},{"success":1,"file_name":"Extdata_01_d_1.tif","relation":"main_file","content_type":"image/tiff","file_size":433981,"creator":"jdanzl","date_updated":"2023-08-03T12:05:41Z","file_id":"13576","checksum":"52f43300a50f83f9a6ff1409324086ee","date_created":"2023-08-03T12:05:41Z","access_level":"open_access"},{"file_name":"Extdata_01_d_2.tif","success":1,"creator":"jdanzl","file_size":19649,"relation":"main_file","content_type":"image/tiff","checksum":"28925dcf8792f524e286beb16b0c532c","file_id":"13577","date_updated":"2023-08-03T12:05:41Z","access_level":"open_access","date_created":"2023-08-03T12:05:41Z"},{"access_level":"open_access","date_created":"2023-08-03T12:05:41Z","checksum":"c93b2f0426c80d18e252435f1e146568","file_id":"13578","date_updated":"2023-08-03T12:05:41Z","creator":"jdanzl","file_size":433981,"relation":"main_file","content_type":"image/tiff","file_name":"Extdata_01_d_3.tif","success":1},{"file_name":"Extdata_01_d_4.tif","success":1,"creator":"jdanzl","file_size":19649,"relation":"main_file","content_type":"image/tiff","checksum":"c0ed722a768001431f47646ef98c44da","file_id":"13579","date_updated":"2023-08-03T12:05:41Z","access_level":"open_access","date_created":"2023-08-03T12:05:41Z"},{"relation":"main_file","content_type":"image/png","file_size":4752,"creator":"jdanzl","success":1,"file_name":"Extdata_01_f_1.png","date_created":"2023-08-03T12:05:41Z","access_level":"open_access","date_updated":"2023-08-03T12:05:41Z","file_id":"13580","checksum":"db9d386276b985b8e702f797e910bd0b"},{"access_level":"open_access","date_created":"2023-08-03T12:05:41Z","checksum":"244f6645d191c50d997c0dc6a15535ec","date_updated":"2023-08-03T12:05:41Z","file_id":"13581","creator":"jdanzl","content_type":"image/png","relation":"main_file","file_size":2698,"success":1,"file_name":"Extdata_01_f_2.png"},{"checksum":"3d6100b836d850b38e66e0f5a7919495","file_id":"13582","date_updated":"2023-08-03T12:07:27Z","access_level":"open_access","date_created":"2023-08-03T12:07:27Z","file_name":"Extdata_02_a.tif","success":1,"creator":"jdanzl","file_size":24003247,"content_type":"image/tiff","relation":"main_file"},{"checksum":"3cd6a3b7bf2061cf5c7810c0001236e5","file_id":"13583","date_updated":"2023-08-03T12:07:27Z","access_level":"open_access","date_created":"2023-08-03T12:07:27Z","file_name":"Extdata_02_b.tif","success":1,"creator":"jdanzl","file_size":1164723,"content_type":"image/tiff","relation":"main_file"},{"creator":"jdanzl","file_size":7109763,"content_type":"image/tiff","relation":"main_file","file_name":"Extdata_03_SYPH1_xy_1.tif","success":1,"access_level":"open_access","date_created":"2023-08-03T12:10:32Z","checksum":"d87c82d663ffea379cc86c56981e2e16","file_id":"13584","date_updated":"2023-08-03T12:10:32Z"},{"file_id":"13585","date_updated":"2023-08-03T12:10:32Z","checksum":"a3703d0d26393578d4ba466681fc6be8","date_created":"2023-08-03T12:10:32Z","access_level":"open_access","file_name":"Extdata_03_SYPH1_xz_1-2.tif","success":1,"file_size":27791,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl"},{"file_name":"Extdata_03_SYPH1_xz_3-4.tif","success":1,"file_size":27721,"content_type":"image/tiff","relation":"main_file","creator":"jdanzl","file_id":"13586","date_updated":"2023-08-03T12:10:32Z","checksum":"f653ac7d531cf9896cbd6a9edf2a7759","date_created":"2023-08-03T12:10:32Z","access_level":"open_access"},{"creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":57657482,"success":1,"file_name":"Extdata_03_SYPH1_xz_n2v.tif","access_level":"open_access","date_created":"2023-08-03T12:10:33Z","checksum":"04edbb337df4d5470e033a4f53c07300","date_updated":"2023-08-03T12:10:33Z","file_id":"13587"},{"checksum":"09e1fe2dd3a1e8349693fda55d0985ca","file_id":"13588","date_updated":"2023-08-03T12:10:34Z","access_level":"open_access","date_created":"2023-08-03T12:10:34Z","file_name":"Extdata_03_SYPH1_xz.tif","success":1,"creator":"jdanzl","file_size":57657527,"relation":"main_file","content_type":"image/tiff"},{"checksum":"458f8d486cb63876c6664cde4a9be27c","file_id":"13589","date_updated":"2023-08-03T12:12:25Z","access_level":"open_access","date_created":"2023-08-03T12:12:25Z","file_name":"Extdata_03_SYP1_xy_1.tif","success":1,"creator":"jdanzl","file_size":7109763,"content_type":"image/tiff","relation":"main_file"},{"checksum":"e2dfccc882d85b660d65a596e1a8cbac","file_id":"13590","date_updated":"2023-08-03T12:12:25Z","access_level":"open_access","date_created":"2023-08-03T12:12:25Z","file_name":"Extdata_03_SYP1_xy_2-3.tif","success":1,"creator":"jdanzl","file_size":72963,"content_type":"image/tiff","relation":"main_file"},{"file_name":"Extdata_03_SYP1_xz_1-2.tif","success":1,"creator":"jdanzl","file_size":27724,"content_type":"image/tiff","relation":"main_file","checksum":"a331be6052c68cc027cdb79f15447d9a","file_id":"13591","date_updated":"2023-08-03T12:12:25Z","access_level":"open_access","date_created":"2023-08-03T12:12:25Z"},{"access_level":"open_access","date_created":"2023-08-03T12:12:25Z","checksum":"4de626b035b43feaacd923fca411e6a2","file_id":"13592","date_updated":"2023-08-03T12:12:25Z","creator":"jdanzl","file_size":27724,"content_type":"image/tiff","relation":"main_file","file_name":"Extdata_03_SYP1_xz_3-4.tif","success":1},{"date_updated":"2023-08-03T12:12:30Z","file_id":"13593","checksum":"d8076e927a074f024924d9bdb6fdd5e6","date_created":"2023-08-03T12:12:30Z","access_level":"open_access","success":1,"file_name":"Extdata_03_SYP1_xz_n2v.tif","content_type":"image/tiff","relation":"main_file","file_size":214613644,"creator":"jdanzl"},{"date_created":"2023-08-03T12:12:30Z","access_level":"open_access","file_id":"13594","date_updated":"2023-08-03T12:12:30Z","checksum":"79cf7d0f450c397caa877dd06b52f8df","file_size":214613687,"content_type":"image/tiff","relation":"main_file","creator":"jdanzl","file_name":"Extdata_03_SYP1_xz.tif","success":1},{"success":1,"file_name":"Extdata03_VAMP2_xy_1.tif","creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":7109747,"checksum":"60ac6ea988d9b8bdaba2f09e0e1ef370","date_updated":"2023-08-03T12:14:29Z","file_id":"13595","access_level":"open_access","date_created":"2023-08-03T12:14:29Z"},{"access_level":"open_access","date_created":"2023-08-03T12:14:29Z","checksum":"92bf312da2dd356aa4fc9a0e7d811af2","date_updated":"2023-08-03T12:14:29Z","file_id":"13596","creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":72947,"success":1,"file_name":"Extdata03_VAMP2_xy_2-3.tif"},{"creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":27724,"success":1,"file_name":"Extdata03_VAMP2_xz_1-2.tif","access_level":"open_access","date_created":"2023-08-03T12:14:29Z","checksum":"9319e293092eeee24e3c658fc178af1a","date_updated":"2023-08-03T12:14:29Z","file_id":"13597"},{"checksum":"a627d7fd9a89bdde968e6d9bff846254","file_id":"13598","date_updated":"2023-08-03T12:14:29Z","access_level":"open_access","date_created":"2023-08-03T12:14:29Z","file_name":"Extdata03_VAMP2_xz_3-4.tif","success":1,"creator":"jdanzl","file_size":27708,"content_type":"image/tiff","relation":"main_file"},{"checksum":"7a5363a185edce59ce57535f7d855092","file_id":"13599","date_updated":"2023-08-03T12:14:32Z","access_level":"open_access","date_created":"2023-08-03T12:14:32Z","file_name":"Extdata03_VAMP2_xz_n2v.tif","success":1,"creator":"jdanzl","file_size":102457485,"content_type":"image/tiff","relation":"main_file"},{"date_created":"2023-08-03T12:14:32Z","access_level":"open_access","file_id":"13600","date_updated":"2023-08-03T12:14:32Z","checksum":"8e7fc4d00fff8ec6d9340e969f37e176","file_size":102457527,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl","file_name":"Extdata03_VAMP2_xz.tif","success":1},{"date_created":"2023-08-03T12:16:08Z","access_level":"open_access","file_id":"13601","date_updated":"2023-08-03T12:16:08Z","checksum":"be17472f73ed8cd02a700b861f1dbac0","file_size":11117763,"content_type":"image/tiff","relation":"main_file","creator":"jdanzl","file_name":"Extdata04_VGLUT_xy_1.tif","success":1},{"creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":72947,"success":1,"file_name":"Extdata04_VGLUT_xy_2-3.tif","access_level":"open_access","date_created":"2023-08-03T12:16:08Z","checksum":"dbc3ec477f491ce3e98ab40475bd90ca","date_updated":"2023-08-03T12:16:08Z","file_id":"13602"},{"file_id":"13603","date_updated":"2023-08-03T12:16:08Z","checksum":"8ee877e6b843bb0e38febb0481962d69","date_created":"2023-08-03T12:16:08Z","access_level":"open_access","file_name":"Extdata04_VGLUT_xz_1-2.tif","success":1,"file_size":53367,"content_type":"image/tiff","relation":"main_file","creator":"jdanzl"},{"access_level":"open_access","date_created":"2023-08-03T12:16:08Z","checksum":"9873f54d50955a2c204d0559c87aa13d","file_id":"13604","date_updated":"2023-08-03T12:16:08Z","creator":"jdanzl","file_size":53358,"content_type":"image/tiff","relation":"main_file","file_name":"Extdata04_VGLUT_xz_3-4.tif","success":1},{"date_created":"2023-08-03T12:16:11Z","access_level":"open_access","file_id":"13605","date_updated":"2023-08-03T12:16:11Z","checksum":"8eda08ae5981fcfa3758d0320b1923af","file_size":144076208,"content_type":"image/tiff","relation":"main_file","creator":"jdanzl","file_name":"Extdata04_VGLUT_xz_n2v.tif","success":1},{"checksum":"bc0b10229d498f60996a5b05cb87c428","date_updated":"2023-08-03T12:16:10Z","file_id":"13606","access_level":"open_access","date_created":"2023-08-03T12:16:10Z","success":1,"file_name":"Extdata04_VGLUT_xz.tif","creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":72071440},{"access_level":"open_access","date_created":"2023-08-03T12:32:32Z","checksum":"e8d093ce7d98ea5647d8a80e3084c40b","file_id":"13607","date_updated":"2023-08-03T12:32:32Z","creator":"jdanzl","file_size":7109763,"relation":"main_file","content_type":"image/tiff","file_name":"Extdata03_VGAT_xy_1.tif","success":1},{"creator":"jdanzl","file_size":72947,"content_type":"image/tiff","relation":"main_file","file_name":"Extdata03_VGAT_xy_2-3.tif","success":1,"access_level":"open_access","date_created":"2023-08-03T12:32:32Z","checksum":"36590ca7a586de7ad76d3ad659c38995","file_id":"13608","date_updated":"2023-08-03T12:32:32Z"},{"date_created":"2023-08-03T12:32:32Z","access_level":"open_access","date_updated":"2023-08-03T12:32:32Z","file_id":"13609","checksum":"3914c7709f69d4d18f23a9fb6dc64572","relation":"main_file","content_type":"image/tiff","file_size":53355,"creator":"jdanzl","success":1,"file_name":"Extdata03_VGAT_xz_1-2.tif"},{"date_created":"2023-08-03T12:32:32Z","access_level":"open_access","date_updated":"2023-08-03T12:32:32Z","file_id":"13610","checksum":"fa98aa521adaefb9e4476fe519e88e59","content_type":"image/tiff","relation":"main_file","file_size":53339,"creator":"jdanzl","success":1,"file_name":"Extdata03_VGAT_xz_3-4.tif"},{"file_id":"13611","date_updated":"2023-08-03T12:32:36Z","checksum":"cd6f384c51756977e37f45043c913252","date_created":"2023-08-03T12:32:36Z","access_level":"open_access","file_name":"Extdata03_VGAT_xz_n2v.tif","success":1,"file_size":204861338,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl"},{"creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":102457527,"success":1,"file_name":"Extdata03_VGAT_xz.tif","access_level":"open_access","date_created":"2023-08-03T12:32:35Z","checksum":"fb7e46feda5ac07877a91ad963da0e31","date_updated":"2023-08-03T12:32:35Z","file_id":"13612"},{"date_created":"2023-08-03T12:36:46Z","access_level":"open_access","file_id":"13613","date_updated":"2023-08-03T12:36:46Z","checksum":"fe033e0a377d391cd8e4af04987d9900","file_size":4348932,"content_type":"image/png","relation":"main_file","creator":"jdanzl","file_name":"Extdata04_MUNC_xy_1.png","success":1},{"access_level":"open_access","date_created":"2023-08-03T12:35:58Z","checksum":"771d8e2b9192b6620f54ab30e4fad594","date_updated":"2023-08-03T12:35:58Z","file_id":"13614","creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":72947,"success":1,"file_name":"Extdata04_MUNC_xy_2-3.tif"},{"date_updated":"2023-08-03T12:35:58Z","file_id":"13615","checksum":"4256d8f06fa2077b352776cf76b46618","date_created":"2023-08-03T12:35:58Z","access_level":"open_access","success":1,"file_name":"Extdata04_MUNC_xz_1-2.tif","content_type":"image/tiff","relation":"main_file","file_size":53435,"creator":"jdanzl"},{"file_name":"Extdata04_MUNC_xz_3-4.tif","success":1,"file_size":55941,"content_type":"image/tiff","relation":"main_file","creator":"jdanzl","file_id":"13616","date_updated":"2023-08-03T12:35:58Z","checksum":"0b9365638c81d545e773170cf04b04b8","date_created":"2023-08-03T12:35:58Z","access_level":"open_access"},{"creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":204861404,"success":1,"file_name":"Extdata04_MUNC_xz_n2v.tif","access_level":"open_access","date_created":"2023-08-03T12:36:04Z","checksum":"9fa62688dab03c592cec47bf5d1c304d","date_updated":"2023-08-03T12:36:04Z","file_id":"13617"},{"file_size":102457527,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl","file_name":"Extdata04_MUNC_xz.tif","success":1,"date_created":"2023-08-03T12:36:02Z","access_level":"open_access","file_id":"13618","date_updated":"2023-08-03T12:36:02Z","checksum":"39f710080a26136dbbe4c8916b434c65"},{"access_level":"open_access","date_created":"2023-08-03T12:38:39Z","checksum":"eaabc8112645a73f4326e621b4e6ad0a","file_id":"13619","date_updated":"2023-08-03T12:38:39Z","creator":"jdanzl","file_size":11117757,"content_type":"image/tiff","relation":"main_file","file_name":"Extdata04_BASSOON_xy_1.tif","success":1},{"file_name":"Extdata04_BASSOON_xy_2-3.tif","success":1,"creator":"jdanzl","file_size":74041,"content_type":"image/tiff","relation":"main_file","checksum":"15f624d29c473ce6e0ae7e466efc059a","file_id":"13620","date_updated":"2023-08-03T12:38:39Z","access_level":"open_access","date_created":"2023-08-03T12:38:39Z"},{"creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":53435,"success":1,"file_name":"Extdata04_BASSOON_xz_1-2.tif","access_level":"open_access","date_created":"2023-08-03T12:38:39Z","checksum":"1cb4b4d219767a30072c804a07140240","date_updated":"2023-08-03T12:38:39Z","file_id":"13621"},{"date_created":"2023-08-03T12:38:39Z","access_level":"open_access","file_id":"13622","date_updated":"2023-08-03T12:38:39Z","checksum":"1bac9c192da9375d81cb3747f48c9be4","file_size":53362,"content_type":"image/tiff","relation":"main_file","creator":"jdanzl","file_name":"Extdata04_BASSOON_xz_3-4.tif","success":1},{"date_updated":"2023-08-03T12:38:50Z","file_id":"13623","checksum":"57fa91bfb1e2937e3449e57096b6fc74","date_created":"2023-08-03T12:38:50Z","access_level":"open_access","success":1,"file_name":"Extdata04_BASSOON_xz_n2v.tif","relation":"main_file","content_type":"image/tiff","file_size":576071410,"creator":"jdanzl"},{"success":1,"file_name":"Extdata04_BASSOON_xz.tif","creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":288066640,"checksum":"ac51a734dcf7d1b94afcd4a471f10626","date_updated":"2023-08-03T12:38:46Z","file_id":"13624","access_level":"open_access","date_created":"2023-08-03T12:38:46Z"},{"file_size":7109763,"content_type":"image/tiff","relation":"main_file","creator":"jdanzl","file_name":"Extdata04_HOMER1_xy_1.tif","success":1,"date_created":"2023-08-03T12:40:57Z","access_level":"open_access","file_id":"13625","date_updated":"2023-08-03T12:40:57Z","checksum":"244043f7acba250d560e29123702dcd7"},{"success":1,"file_name":"Extdata04_HOMER1_xy_2-3.tif","relation":"main_file","content_type":"image/tiff","file_size":72963,"creator":"jdanzl","date_updated":"2023-08-03T12:40:57Z","file_id":"13626","checksum":"eff51ff97539f6491989bfc3d3f88860","date_created":"2023-08-03T12:40:57Z","access_level":"open_access"},{"checksum":"df35046d9bca4e97a5625c5a55218cfb","date_updated":"2023-08-03T12:40:57Z","file_id":"13627","access_level":"open_access","date_created":"2023-08-03T12:40:57Z","success":1,"file_name":"Extdata04_HOMER1_xz_1-2.tif","creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":53375},{"checksum":"5dc7536f7aaea084dfc329537d8412ce","file_id":"13628","date_updated":"2023-08-03T12:40:57Z","access_level":"open_access","date_created":"2023-08-03T12:40:57Z","file_name":"Extdata04_HOMER1_xz_3-4.tif","success":1,"creator":"jdanzl","file_size":53359,"content_type":"image/tiff","relation":"main_file"},{"access_level":"open_access","date_created":"2023-08-03T12:41:00Z","checksum":"0b407669a26b9d7730a41361ceffda9a","file_id":"13629","date_updated":"2023-08-03T12:41:00Z","creator":"jdanzl","file_size":204861339,"content_type":"image/tiff","relation":"main_file","file_name":"Extdata04_HOMER1_xz_n2v.tif","success":1},{"creator":"jdanzl","file_size":102457527,"relation":"main_file","content_type":"image/tiff","file_name":"Extdata04_HOMER1_xz.tif","success":1,"access_level":"open_access","date_created":"2023-08-03T12:40:59Z","checksum":"f95a0da9bea8b605fc2ed310190836b3","file_id":"13630","date_updated":"2023-08-03T12:40:59Z"},{"file_name":"Extdata04_SHANK2_xy_1.tif","success":1,"creator":"jdanzl","file_size":7120361,"relation":"main_file","content_type":"image/tiff","checksum":"cf74307696548e7aa90e59a0935a74a1","file_id":"13631","date_updated":"2023-08-03T12:45:08Z","access_level":"open_access","date_created":"2023-08-03T12:45:08Z"},{"file_name":"Extdata04_SHANK2_xy_2-3.tif","success":1,"creator":"jdanzl","file_size":72973,"relation":"main_file","content_type":"image/tiff","checksum":"7b3490246df4f5bb68d0df682161820f","file_id":"13632","date_updated":"2023-08-03T12:45:08Z","access_level":"open_access","date_created":"2023-08-03T12:45:08Z"},{"access_level":"open_access","date_created":"2023-08-03T12:45:08Z","checksum":"c599d6830e61c386219e266f4717fe7c","file_id":"13633","date_updated":"2023-08-03T12:45:08Z","creator":"jdanzl","file_size":53443,"relation":"main_file","content_type":"image/tiff","file_name":"Extdata04_SHANK2_xz_1-2.tif","success":1},{"date_created":"2023-08-03T12:45:08Z","access_level":"open_access","file_id":"13634","date_updated":"2023-08-03T12:45:08Z","checksum":"5b9912b2a408d224ad669f39dc8d5bc6","file_size":53362,"content_type":"image/tiff","relation":"main_file","creator":"jdanzl","file_name":"Extdata04_SHANK2_xz_3-4.tif","success":1},{"access_level":"open_access","date_created":"2023-08-03T12:45:30Z","checksum":"17f2064ceeb99cbb51208c27b1961ed5","date_updated":"2023-08-03T12:45:30Z","file_id":"13635","creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":691291157,"success":1,"file_name":"Extdata04_SHANK2_xz_n2v.tif"},{"success":1,"file_name":"Extdata04_SHANK2_xz.tif","creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":518519275,"checksum":"21f1bb8d2a00c88bdd6febe6285acc2a","date_updated":"2023-08-03T12:45:26Z","file_id":"13636","access_level":"open_access","date_created":"2023-08-03T12:45:26Z"},{"file_size":752109,"content_type":"image/tiff","relation":"main_file","creator":"jdanzl","file_name":"Extdata04_GEPHYRIN_xy_1.tif","success":1,"date_created":"2023-08-03T12:46:25Z","access_level":"open_access","file_id":"13637","date_updated":"2023-08-03T12:46:25Z","checksum":"47597ca873f1ea81cd4f7e9bfbb34aa4"},{"checksum":"14ed7ae64eb783e6b30f824f21959d0f","file_id":"13638","date_updated":"2023-08-03T12:46:25Z","access_level":"open_access","date_created":"2023-08-03T12:46:25Z","file_name":"Extdata04_GEPHYRIN_xy_2-3.tif","success":1,"creator":"jdanzl","file_size":72909,"relation":"main_file","content_type":"image/tiff"},{"success":1,"file_name":"Extdata04_GEPHYRIN_xz_1-2.tif","relation":"main_file","content_type":"image/tiff","file_size":53375,"creator":"jdanzl","date_updated":"2023-08-03T12:46:25Z","file_id":"13639","checksum":"e361e226428d7b23618eed35b1cad080","date_created":"2023-08-03T12:46:25Z","access_level":"open_access"},{"access_level":"open_access","date_created":"2023-08-03T12:46:25Z","checksum":"abe7cee22f69fc2bf1a3ff9955ccf45a","file_id":"13640","date_updated":"2023-08-03T12:46:25Z","creator":"jdanzl","file_size":53360,"content_type":"image/tiff","relation":"main_file","file_name":"Extdata04_GEPHYRIN_xz_3-4.tif","success":1},{"file_id":"13641","date_updated":"2023-08-03T12:46:27Z","checksum":"59b49b451a63c5ac06fa31a17640c1a2","date_created":"2023-08-03T12:46:27Z","access_level":"open_access","file_name":"Extdata04_GEPHYRIN_xz_n2v.tif","success":1,"file_size":73174939,"content_type":"image/tiff","relation":"main_file","creator":"jdanzl"},{"creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":36614327,"success":1,"file_name":"Extdata04_GEPHYRIN_xz.tif","access_level":"open_access","date_created":"2023-08-03T12:46:26Z","checksum":"1ee1feea85c8319ebc6c49147502e412","date_updated":"2023-08-03T12:46:26Z","file_id":"13642"},{"success":1,"file_name":"Extdata06_a-b_n2v.tif","relation":"main_file","content_type":"image/tiff","file_size":2195652677,"creator":"jdanzl","date_updated":"2023-08-03T12:52:23Z","file_id":"13643","checksum":"64d9d868ef89c5cfc65cb199aff831b4","date_created":"2023-08-03T12:52:23Z","access_level":"open_access"},{"file_name":"Extdata06_a-b.tif","success":1,"creator":"jdanzl","file_size":1097875840,"relation":"main_file","content_type":"image/tiff","checksum":"df41d97987798715b1f66e6e72be169b","file_id":"13644","date_updated":"2023-08-03T12:51:41Z","access_level":"open_access","date_created":"2023-08-03T12:51:41Z"},{"creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":3564577,"success":1,"file_name":"Extdata07_01_bottom.tif","access_level":"open_access","date_created":"2023-08-03T12:52:57Z","checksum":"95a3eaa3b70e964f6871a5765a53659b","date_updated":"2023-08-03T12:52:57Z","file_id":"13645"},{"creator":"jdanzl","file_size":11958844,"content_type":"image/tiff","relation":"main_file","file_name":"Extdata07_01_top.tif","success":1,"access_level":"open_access","date_created":"2023-08-03T12:52:57Z","checksum":"80b41ad0de73fd61eb67a35262627fac","file_id":"13646","date_updated":"2023-08-03T12:52:57Z"},{"date_created":"2023-08-03T12:54:27Z","access_level":"open_access","file_id":"13647","date_updated":"2023-08-03T12:54:27Z","checksum":"f694a0f11e2f782702cb4c411941873a","file_size":3564581,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl","file_name":"Extdata07_2_bottom.tif","success":1},{"access_level":"open_access","date_created":"2023-08-03T12:54:27Z","checksum":"b8b1a1339a25806615c0aa7906a032ff","date_updated":"2023-08-03T12:54:27Z","file_id":"13648","creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":11973592,"success":1,"file_name":"Extdata07_2_top.tif"},{"date_updated":"2023-08-03T12:54:27Z","file_id":"13649","checksum":"8a24eb623d65d809fe8afbd527528a77","date_created":"2023-08-03T12:54:27Z","access_level":"open_access","success":1,"file_name":"Extdata07_3_bottom.tif","relation":"main_file","content_type":"image/tiff","file_size":1281901,"creator":"jdanzl"},{"access_level":"open_access","date_created":"2023-08-03T12:54:27Z","checksum":"816b35def703bc48d55b0293fcd8212f","date_updated":"2023-08-03T12:54:27Z","file_id":"13650","creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":16569776,"success":1,"file_name":"Extdata07_3_top.tif"},{"file_size":3564595,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl","file_name":"Extdata07_04_bottom.tif","success":1,"date_created":"2023-08-03T12:54:27Z","access_level":"open_access","file_id":"13651","date_updated":"2023-08-03T12:54:27Z","checksum":"7f1375ab8cf28cd136e2e44d1fdfd87d"},{"file_id":"13652","date_updated":"2023-08-03T12:54:27Z","checksum":"b1dbda28e2a010968716c0411568f139","date_created":"2023-08-03T12:54:27Z","access_level":"open_access","file_name":"Extdata07_04_top.tif","success":1,"file_size":5896404,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl"},{"date_created":"2023-08-03T12:54:28Z","access_level":"open_access","file_id":"13653","date_updated":"2023-08-03T12:54:28Z","checksum":"d6e96b7c8e6a7084c14eda83a4dd778a","file_size":3564609,"content_type":"image/tiff","relation":"main_file","creator":"jdanzl","file_name":"Extdata07_5_bottom.tif","success":1},{"file_name":"Extdata07_5_top.tif","success":1,"file_size":15555618,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl","file_id":"13654","date_updated":"2023-08-03T12:54:28Z","checksum":"a647f89a3048f71512cc58c9e53ec022","date_created":"2023-08-03T12:54:28Z","access_level":"open_access"},{"access_level":"open_access","date_created":"2023-08-03T12:54:28Z","checksum":"66becb046fe591914df572617d720202","date_updated":"2023-08-03T12:54:28Z","file_id":"13655","creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":3564577,"success":1,"file_name":"Extdata07_6_bottom.tif"},{"checksum":"5d76b2ad7d7acbb1afa27cf52194b1cb","file_id":"13656","date_updated":"2023-08-03T12:54:28Z","access_level":"open_access","date_created":"2023-08-03T12:54:28Z","file_name":"Extdata07_6_top.tif","success":1,"creator":"jdanzl","file_size":6038186,"relation":"main_file","content_type":"image/tiff"},{"date_created":"2023-08-03T12:54:28Z","access_level":"open_access","file_id":"13657","date_updated":"2023-08-03T12:54:28Z","checksum":"7f137bdea0f0acb3db75c983ce44ab44","file_size":3560937,"content_type":"image/tiff","relation":"main_file","creator":"jdanzl","file_name":"Extdata07_7_bottom.tif","success":1},{"success":1,"file_name":"Extdata07_7_top.tif","content_type":"image/tiff","relation":"main_file","file_size":12031850,"creator":"jdanzl","date_updated":"2023-08-03T12:54:28Z","file_id":"13658","checksum":"479e26106280eb61a2d74e29137a2e43","date_created":"2023-08-03T12:54:28Z","access_level":"open_access"},{"checksum":"8426197ad283c1bc83e3193f323d5f5a","file_id":"13659","date_updated":"2023-08-03T12:54:28Z","access_level":"open_access","date_created":"2023-08-03T12:54:28Z","file_name":"Extdata07_8_bottom.tif","success":1,"creator":"jdanzl","file_size":3560937,"content_type":"image/tiff","relation":"main_file"},{"content_type":"image/tiff","relation":"main_file","file_size":22877176,"creator":"jdanzl","success":1,"file_name":"Extdata07_8_top.tif","date_created":"2023-08-03T12:54:28Z","access_level":"open_access","date_updated":"2023-08-03T12:54:28Z","file_id":"13660","checksum":"b9f75a82b564e1c5c363a495d078f6b7"},{"success":1,"file_name":"Extdata07_9_bottom.tif","relation":"main_file","content_type":"image/tiff","file_size":3564605,"creator":"jdanzl","date_updated":"2023-08-03T12:54:28Z","file_id":"13661","checksum":"7f1a9f3bb9611362995ae832b2607f4d","date_created":"2023-08-03T12:54:28Z","access_level":"open_access"},{"creator":"jdanzl","file_size":2744190,"content_type":"image/tiff","relation":"main_file","file_name":"Extdata07_9_top.tif","success":1,"access_level":"open_access","date_created":"2023-08-03T12:54:28Z","checksum":"40286bcb876b655d7421534d0215e86b","file_id":"13662","date_updated":"2023-08-03T12:54:28Z"},{"access_level":"open_access","date_created":"2023-08-03T13:00:03Z","checksum":"4af220986cc94d412c2354aa7e6c8cce","file_id":"13663","date_updated":"2023-08-03T13:00:03Z","creator":"jdanzl","file_size":7243611,"content_type":"image/png","relation":"main_file","file_name":"Extdata08_6_left.png","success":1},{"file_name":"Extdata08_6_right.tif","success":1,"file_size":5562487,"content_type":"image/tiff","relation":"main_file","creator":"jdanzl","file_id":"13664","date_updated":"2023-08-03T13:00:03Z","checksum":"af9c2299f1005a3ccc39f41082cea642","date_created":"2023-08-03T13:00:03Z","access_level":"open_access"},{"access_level":"open_access","date_created":"2023-08-03T13:00:04Z","checksum":"63ef28a174215c70e31d2cabf67d90fc","date_updated":"2023-08-03T13:00:04Z","file_id":"13665","creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":34745956,"success":1,"file_name":"Extdata08_7_left.tif"},{"success":1,"file_name":"Extdata08_7_right.tif","creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":5562483,"checksum":"46de918dfe5f3c23e497ee3daa608dd9","date_updated":"2023-08-03T13:00:03Z","file_id":"13666","access_level":"open_access","date_created":"2023-08-03T13:00:03Z"},{"file_name":"Extdata08_b_1_left.tif","success":1,"creator":"jdanzl","file_size":31506150,"content_type":"image/tiff","relation":"main_file","checksum":"8243a7985d2902446183adc35519a0c8","file_id":"13667","date_updated":"2023-08-03T13:00:04Z","access_level":"open_access","date_created":"2023-08-03T13:00:04Z"},{"file_name":"Extdata08_b_1_right.tif","success":1,"creator":"jdanzl","file_size":11117705,"relation":"main_file","content_type":"image/tiff","checksum":"857cffa31569efcd5917f9802a106d58","file_id":"13668","date_updated":"2023-08-03T13:00:04Z","access_level":"open_access","date_created":"2023-08-03T13:00:04Z"},{"success":1,"file_name":"Extdata08_b_2_left.png","creator":"jdanzl","relation":"main_file","content_type":"image/png","file_size":3540493,"checksum":"e34902de9119d2cd9f1910f610627c5f","date_updated":"2023-08-03T13:00:04Z","file_id":"13669","access_level":"open_access","date_created":"2023-08-03T13:00:04Z"},{"file_id":"13670","date_updated":"2023-08-03T13:00:05Z","checksum":"1247d636ac5af22e8950ffd2ff213fea","date_created":"2023-08-03T13:00:05Z","access_level":"open_access","file_name":"Extdata08_b_2_right.tif","success":1,"file_size":5562553,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl"},{"relation":"main_file","content_type":"image/png","file_size":3541547,"creator":"jdanzl","success":1,"file_name":"Extdata08_b_3_left.png","date_created":"2023-08-03T13:00:05Z","access_level":"open_access","date_updated":"2023-08-03T13:00:05Z","file_id":"13671","checksum":"f47c2d3f6824e08853ae2631f6ee7c5f"},{"date_updated":"2023-08-03T13:00:05Z","file_id":"13672","checksum":"1247d636ac5af22e8950ffd2ff213fea","date_created":"2023-08-03T13:00:05Z","access_level":"open_access","success":1,"file_name":"Extdata08_b_3_right.tif","relation":"main_file","content_type":"image/tiff","file_size":5562553,"creator":"jdanzl"},{"success":1,"file_name":"Extdata08_b_4_left.tif","relation":"main_file","content_type":"image/tiff","file_size":25819844,"creator":"jdanzl","date_updated":"2023-08-03T13:00:05Z","file_id":"13673","checksum":"e90a95ec902a683678acd70336852fd9","date_created":"2023-08-03T13:00:05Z","access_level":"open_access"},{"checksum":"94cd44cd1c8c7a87ffd5861bf88561a1","date_updated":"2023-08-03T13:00:05Z","file_id":"13674","access_level":"open_access","date_created":"2023-08-03T13:00:05Z","success":1,"file_name":"Extdata08_b_4_right.tif","creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":5561131},{"creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":38786287,"success":1,"file_name":"Extdata08_b_5_left.tif","access_level":"open_access","date_created":"2023-08-03T13:00:06Z","checksum":"fd4ca56dc5c48ad4546df22daef04efc","date_updated":"2023-08-03T13:00:06Z","file_id":"13675"},{"date_updated":"2023-08-03T13:00:06Z","file_id":"13676","checksum":"52c04890160c4e0f23d3fe2d3ed0c8e7","date_created":"2023-08-03T13:00:06Z","access_level":"open_access","success":1,"file_name":"Extdata08_b_5_right.tif","content_type":"image/tiff","relation":"main_file","file_size":5562487,"creator":"jdanzl"},{"file_size":2133527,"content_type":"image/png","relation":"main_file","creator":"jdanzl","file_name":"Extdata08_a_1-1.png","success":1,"date_created":"2023-08-03T13:04:52Z","access_level":"open_access","file_id":"13677","date_updated":"2023-08-03T13:04:52Z","checksum":"47ee6f4b083130d5bb720bcf8a832443"},{"file_name":"Extdata08_a_1-2.png","success":1,"creator":"jdanzl","file_size":1782607,"content_type":"image/png","relation":"main_file","checksum":"5ca2279c372b3b7dbce3ea5658a68399","file_id":"13678","date_updated":"2023-08-03T13:04:52Z","access_level":"open_access","date_created":"2023-08-03T13:04:52Z"},{"access_level":"open_access","date_created":"2023-08-03T13:04:52Z","checksum":"34b7781e3e4abf9f30a0c85db156b6d5","file_id":"13679","date_updated":"2023-08-03T13:04:52Z","creator":"jdanzl","file_size":5117469,"relation":"main_file","content_type":"image/png","file_name":"Extdata08_a_2.png","success":1},{"access_level":"open_access","date_created":"2023-08-03T13:04:52Z","checksum":"25717007393682098d2208bd0676a0f7","date_updated":"2023-08-03T13:04:52Z","file_id":"13680","creator":"jdanzl","relation":"main_file","content_type":"image/png","file_size":6911658,"success":1,"file_name":"Extdata08_a_3.png"},{"creator":"jdanzl","file_size":8579427,"content_type":"image/png","relation":"main_file","file_name":"Extdata08_a_4.png","success":1,"access_level":"open_access","date_created":"2023-08-03T13:04:53Z","checksum":"b4b003809acec33c71628066744c7ea9","file_id":"13681","date_updated":"2023-08-03T13:04:53Z"},{"success":1,"file_name":"Extdata08_a_5.png","creator":"jdanzl","content_type":"image/png","relation":"main_file","file_size":9675065,"checksum":"4410976cf867ecacf8f197dbd28cc5a0","date_updated":"2023-08-03T13:04:53Z","file_id":"13682","access_level":"open_access","date_created":"2023-08-03T13:04:53Z"},{"date_updated":"2023-08-03T13:04:53Z","file_id":"13683","checksum":"a235dd204f28d3323883e044b031b984","date_created":"2023-08-03T13:04:53Z","access_level":"open_access","success":1,"file_name":"Extdata08_a_6.png","content_type":"image/png","relation":"main_file","file_size":9769588,"creator":"jdanzl"},{"checksum":"0e2b768e4eb7200cf6fe28ba511a5ef6","file_id":"13684","date_updated":"2023-08-03T13:04:53Z","access_level":"open_access","date_created":"2023-08-03T13:04:53Z","file_name":"Extdata08_a_7.png","success":1,"creator":"jdanzl","file_size":9114791,"relation":"main_file","content_type":"image/png"},{"success":1,"file_name":"Extdata08_a_8.png","relation":"main_file","content_type":"image/png","file_size":124540688,"creator":"jdanzl","date_updated":"2023-08-03T13:04:57Z","file_id":"13685","checksum":"df4a01c62233ee8ed601c4b90d7780d0","date_created":"2023-08-03T13:04:57Z","access_level":"open_access"},{"success":1,"file_name":"Exdata09_c_n2v.tif","creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":204861336,"checksum":"1f407c7915e2717a56fec258708e7b7a","date_updated":"2023-08-03T13:11:30Z","file_id":"13686","access_level":"open_access","date_created":"2023-08-03T13:11:30Z"},{"checksum":"9089dc7c9914edf9750eebd65819708d","file_id":"13687","date_updated":"2023-08-03T13:11:26Z","access_level":"open_access","date_created":"2023-08-03T13:11:26Z","file_name":"Extdata09_a_zoom.tif","success":1,"creator":"jdanzl","file_size":252149,"content_type":"image/tiff","relation":"main_file"},{"success":1,"file_name":"Extdata09_a.tif","relation":"main_file","content_type":"image/tiff","file_size":11117705,"creator":"jdanzl","date_updated":"2023-08-03T13:11:26Z","file_id":"13688","checksum":"62d295a62cb8dd36c268b28cf0251322","date_created":"2023-08-03T13:11:26Z","access_level":"open_access"},{"date_updated":"2023-08-03T13:11:31Z","file_id":"13689","checksum":"e874d8ee287f924c1076134b5b06bdb2","date_created":"2023-08-03T13:11:31Z","access_level":"open_access","success":1,"file_name":"Extdata09_b_n2v.tif","relation":"main_file","content_type":"image/tiff","file_size":256074660,"creator":"jdanzl"},{"creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":127914854,"success":1,"file_name":"Extdata10_n2v_CLAHE.tif","access_level":"open_access","date_created":"2023-08-03T13:12:53Z","checksum":"488068fff7b42bcf5728739e2a06d742","date_updated":"2023-08-03T13:12:53Z","file_id":"13690"},{"date_created":"2023-08-03T13:21:35Z","access_level":"open_access","file_id":"13691","date_updated":"2023-08-03T13:21:35Z","checksum":"35351f5af21ce854d41577813a7bdb57","file_size":430878,"relation":"main_file","content_type":"image/png","creator":"jdanzl","file_name":"Supplfig01_a_1.png","success":1},{"success":1,"file_name":"Supplfig01_a_2.png","creator":"jdanzl","relation":"main_file","content_type":"image/png","file_size":438843,"checksum":"36204e9e604206366ffc6f61ec91915b","date_updated":"2023-08-03T13:21:35Z","file_id":"13692","access_level":"open_access","date_created":"2023-08-03T13:21:35Z"},{"date_created":"2023-08-03T13:21:35Z","access_level":"open_access","date_updated":"2023-08-03T13:21:35Z","file_id":"13693","checksum":"92ccc934c4603b29f4f7a96573e49c83","relation":"main_file","content_type":"image/png","file_size":426713,"creator":"jdanzl","success":1,"file_name":"Supplfig01_a_3.png"},{"success":1,"file_name":"Supplfig01_a_4.tif","content_type":"image/tiff","relation":"main_file","file_size":723221,"creator":"jdanzl","date_updated":"2023-08-03T13:21:35Z","file_id":"13694","checksum":"e71faa54934bee8ac3e66ce692f542ab","date_created":"2023-08-03T13:21:35Z","access_level":"open_access"},{"file_name":"Supplfig01_a_5.tif","success":1,"creator":"jdanzl","file_size":723235,"relation":"main_file","content_type":"image/tiff","checksum":"95dc33563f48b650e5ac020bdfd10c48","file_id":"13695","date_updated":"2023-08-03T13:21:35Z","access_level":"open_access","date_created":"2023-08-03T13:21:35Z"},{"date_created":"2023-08-03T13:21:35Z","access_level":"open_access","date_updated":"2023-08-03T13:21:35Z","file_id":"13696","checksum":"c62df0a9faf1c47af121d77eb1582edd","content_type":"image/tiff","relation":"main_file","file_size":1442129,"creator":"jdanzl","success":1,"file_name":"Supplfig01_b_1.tif"},{"checksum":"8ae157c5a4a543da1193cb8ca12b1919","date_updated":"2023-08-03T13:21:35Z","file_id":"13697","access_level":"open_access","date_created":"2023-08-03T13:21:35Z","success":1,"file_name":"Supplfig01_b_2.tif","creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":725407},{"checksum":"388370d954ce842781c0d62edfb448a7","date_updated":"2023-08-03T13:21:35Z","file_id":"13698","access_level":"open_access","date_created":"2023-08-03T13:21:35Z","success":1,"file_name":"Supplfig01_c_1.tif","creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":721641},{"access_level":"open_access","date_created":"2023-08-03T13:21:35Z","checksum":"6cae58ba3c98bc8253e1c769e93657a7","date_updated":"2023-08-03T13:21:35Z","file_id":"13699","creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":6003079,"success":1,"file_name":"Supplfig01_c_2.tif"},{"access_level":"open_access","date_created":"2023-08-03T13:21:35Z","checksum":"85cf013232c72895aefc07f5411a765a","file_id":"13700","date_updated":"2023-08-03T13:21:35Z","creator":"jdanzl","file_size":2003311,"content_type":"image/tiff","relation":"main_file","file_name":"Supplfig01_c_3.tif","success":1},{"date_created":"2023-08-03T13:21:35Z","access_level":"open_access","date_updated":"2023-08-03T13:21:35Z","file_id":"13701","checksum":"dfb33b91066e3f34d3803f59a4105aba","content_type":"image/tiff","relation":"main_file","file_size":723271,"creator":"jdanzl","success":1,"file_name":"Supplfig01_c_4.tif"},{"file_name":"Supplfig01_d_1.tif","success":1,"creator":"jdanzl","file_size":721901,"relation":"main_file","content_type":"image/tiff","checksum":"8b899826fe48d7e7536b0b02a2645e87","file_id":"13702","date_updated":"2023-08-03T13:21:35Z","access_level":"open_access","date_created":"2023-08-03T13:21:35Z"},{"checksum":"67529fa6e0f12c272275e823f6045f6d","file_id":"13703","date_updated":"2023-08-03T13:21:35Z","access_level":"open_access","date_created":"2023-08-03T13:21:35Z","file_name":"Supplfig01_d_2.tif","success":1,"creator":"jdanzl","file_size":721901,"content_type":"image/tiff","relation":"main_file"},{"file_id":"13704","date_updated":"2023-08-03T13:21:35Z","checksum":"111a9231f63c775340d3356109715cb0","date_created":"2023-08-03T13:21:35Z","access_level":"open_access","file_name":"Supplfig01_e_1.tif","success":1,"file_size":723223,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl"},{"creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":723223,"success":1,"file_name":"Supplfig01_e_2.tif","access_level":"open_access","date_created":"2023-08-03T13:21:35Z","checksum":"7685bf02408dcb095a00854ff22763ed","date_updated":"2023-08-03T13:21:35Z","file_id":"13705"},{"creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":34821298,"success":1,"file_name":"Supplfig02_b_01.tif","access_level":"open_access","date_created":"2023-08-03T13:26:00Z","checksum":"455f5cb9aa8a87b91586a27aca122610","date_updated":"2023-08-03T13:26:00Z","file_id":"13706"},{"file_name":"Supplfig02_b_02.tif","success":1,"file_size":32131123,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl","file_id":"13707","date_updated":"2023-08-03T13:26:00Z","checksum":"311545b39aaa2c72755fd8ae190803a5","date_created":"2023-08-03T13:26:00Z","access_level":"open_access"},{"content_type":"image/tiff","relation":"main_file","file_size":36872674,"creator":"jdanzl","success":1,"file_name":"Supplfig02_b_03.tif","date_created":"2023-08-03T13:26:01Z","access_level":"open_access","date_updated":"2023-08-03T13:26:01Z","file_id":"13708","checksum":"682deb61f57d9ca54716160ddd52a6cf"},{"file_name":"Supplfig02_b_04.tif","success":1,"file_size":39964115,"content_type":"image/tiff","relation":"main_file","creator":"jdanzl","file_id":"13709","date_updated":"2023-08-03T13:26:01Z","checksum":"4f9579c91b2a3f36ff6e66b592907973","date_created":"2023-08-03T13:26:01Z","access_level":"open_access"},{"success":1,"file_name":"Supplfig02_b_05.tif","relation":"main_file","content_type":"image/tiff","file_size":43053091,"creator":"jdanzl","date_updated":"2023-08-03T13:26:02Z","file_id":"13710","checksum":"7ae7053673b53dbed59ffb92618f969a","date_created":"2023-08-03T13:26:02Z","access_level":"open_access"},{"access_level":"open_access","date_created":"2023-08-03T13:26:02Z","checksum":"827b26d3d2b6ddc9bced9d6113023ced","file_id":"13711","date_updated":"2023-08-03T13:26:02Z","creator":"jdanzl","file_size":46998147,"relation":"main_file","content_type":"image/tiff","file_name":"Supplfig02_b_06.tif","success":1},{"creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":45179219,"success":1,"file_name":"Supplfig02_b_07.tif","access_level":"open_access","date_created":"2023-08-03T13:26:03Z","checksum":"bc2e7e8badb42f8e1facc8b7bc96fdda","date_updated":"2023-08-03T13:26:03Z","file_id":"13712"},{"file_name":"Supplfig02_b_08.tif","success":1,"file_size":46978802,"content_type":"image/tiff","relation":"main_file","creator":"jdanzl","file_id":"13713","date_updated":"2023-08-03T13:26:03Z","checksum":"c2b78aa0f433928afa10a743c5fb73b8","date_created":"2023-08-03T13:26:03Z","access_level":"open_access"},{"checksum":"9cd1ab61cced2c0981e813aeae930855","date_updated":"2023-08-03T13:26:04Z","file_id":"13714","access_level":"open_access","date_created":"2023-08-03T13:26:04Z","success":1,"file_name":"Supplfig02_b_09.tif","creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":48840915},{"file_size":45907059,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl","file_name":"Supplfig02_b_10.tif","success":1,"date_created":"2023-08-03T13:26:04Z","access_level":"open_access","file_id":"13715","date_updated":"2023-08-03T13:26:04Z","checksum":"3086126338aee6ec4ebc1d3350551197"},{"relation":"main_file","content_type":"image/tiff","file_size":32231538,"creator":"jdanzl","success":1,"file_name":"Supplfig02_b_11.tif","date_created":"2023-08-03T13:26:05Z","access_level":"open_access","date_updated":"2023-08-03T13:26:05Z","file_id":"13716","checksum":"69f52c2b3bb5b5f9fef057860b3386fa"},{"creator":"jdanzl","file_size":35264963,"relation":"main_file","content_type":"image/tiff","file_name":"Supplfig02_b_12.tif","success":1,"access_level":"open_access","date_created":"2023-08-03T13:26:05Z","checksum":"defdbeab1e672456bfeafdd1983e779e","file_id":"13717","date_updated":"2023-08-03T13:26:05Z"},{"file_name":"Supplfig03_a_std_xy.png","success":1,"creator":"jdanzl","file_size":63326,"relation":"main_file","content_type":"image/png","checksum":"02c566f781b9ad44f04ce0088a1fa27a","file_id":"13718","date_updated":"2023-08-03T13:28:40Z","access_level":"open_access","date_created":"2023-08-03T13:28:40Z"},{"creator":"jdanzl","file_size":50211,"relation":"main_file","content_type":"image/png","file_name":"Supplfig03_a_std_xz.png","success":1,"access_level":"open_access","date_created":"2023-08-03T13:28:40Z","checksum":"c6c3630ee9a6ed5d207cb68e330da566","file_id":"13719","date_updated":"2023-08-03T13:28:40Z"},{"date_created":"2023-08-03T13:28:41Z","access_level":"open_access","file_id":"13720","date_updated":"2023-08-03T13:28:41Z","checksum":"0dcd4d19e83a7ff259d6aa04d746cd90","file_size":52629,"relation":"main_file","content_type":"image/png","creator":"jdanzl","file_name":"Supplfig03_b_std_xy.png","success":1},{"file_name":"Supplfig03_b_std_xz.png","success":1,"file_size":72245,"content_type":"image/png","relation":"main_file","creator":"jdanzl","file_id":"13721","date_updated":"2023-08-03T13:28:41Z","checksum":"4785de4a6dcb23b25a06b0da29df5bb5","date_created":"2023-08-03T13:28:41Z","access_level":"open_access"},{"date_created":"2023-08-03T13:28:45Z","access_level":"open_access","file_id":"13722","date_updated":"2023-08-03T13:28:45Z","checksum":"19d0f198bd8a25073e45e7950d7a78c2","file_size":98570368,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl","file_name":"Supplfig03_model1.tif","success":1},{"date_created":"2023-08-03T13:28:45Z","access_level":"open_access","file_id":"13723","date_updated":"2023-08-03T13:28:45Z","checksum":"51ff687df4a0867f39b69c2c7a4ee3ca","file_size":98570367,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl","file_name":"Supplfig03_model2.tif","success":1},{"access_level":"open_access","date_created":"2023-08-03T13:28:49Z","checksum":"ec9d9a041ca85fe632c612baf981fcc5","date_updated":"2023-08-03T13:28:49Z","file_id":"13724","creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":98570365,"success":1,"file_name":"Supplfig03_model3.tif"},{"checksum":"a988b15a9529094f1b5519a72871e98e","date_updated":"2023-08-03T13:28:49Z","file_id":"13725","access_level":"open_access","date_created":"2023-08-03T13:28:49Z","success":1,"file_name":"Supplfig03_model4.tif","creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":98570365},{"relation":"main_file","content_type":"image/tiff","file_size":98570369,"creator":"jdanzl","success":1,"file_name":"Supplfig03_model5.tif","date_created":"2023-08-03T13:28:52Z","access_level":"open_access","date_updated":"2023-08-03T13:28:52Z","file_id":"13726","checksum":"4a4fe88fcfd87fcfe118a5a73d81d6b0"},{"file_name":"Supplfig04_a_n2v.tif","success":1,"creator":"jdanzl","file_size":1036927887,"relation":"main_file","content_type":"image/tiff","checksum":"ce9b774ff5163ebfa4e3fb162f4cfffe","file_id":"13727","date_updated":"2023-08-03T13:34:27Z","access_level":"open_access","date_created":"2023-08-03T13:34:27Z"},{"success":1,"file_name":"Supplfig04_a.tif","creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":518519275,"checksum":"21f1bb8d2a00c88bdd6febe6285acc2a","date_updated":"2023-08-03T13:34:13Z","file_id":"13728","access_level":"open_access","date_created":"2023-08-03T13:34:13Z"},{"file_id":"13729","date_updated":"2023-08-03T13:34:22Z","checksum":"503820214ab994c01b18a241f3b62434","date_created":"2023-08-03T13:34:22Z","access_level":"open_access","file_name":"Supplfig04_b.tif","success":1,"file_size":158440069,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl"},{"access_level":"open_access","date_created":"2023-08-03T13:34:37Z","checksum":"e0469102a87332b46904b4ba35b8fb2f","date_updated":"2023-08-03T13:34:37Z","file_id":"13730","creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":309407894,"success":1,"file_name":"Supplfig04_c_n2v.tif"},{"content_type":"image/tiff","relation":"main_file","file_size":154728887,"creator":"jdanzl","success":1,"file_name":"Supplfig04_c.tif","date_created":"2023-08-03T13:34:38Z","access_level":"open_access","date_updated":"2023-08-03T13:34:38Z","file_id":"13731","checksum":"c30a0d18c43045a8fbc5501a37061bd2"},{"file_name":"Supplfig04_d_n2v.tif","success":1,"creator":"jdanzl","file_size":106696749,"relation":"main_file","content_type":"image/tiff","checksum":"69c706710d642b30cda6951494702139","file_id":"13732","date_updated":"2023-08-03T13:34:43Z","access_level":"open_access","date_created":"2023-08-03T13:34:43Z"},{"checksum":"7dbd2e881ebf049352913188fa10224a","date_updated":"2023-08-03T13:34:41Z","file_id":"13733","access_level":"open_access","date_created":"2023-08-03T13:34:41Z","success":1,"file_name":"Supplfig04_d.tif","creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":53367343},{"creator":"jdanzl","file_size":127917734,"content_type":"image/tiff","relation":"main_file","file_name":"Supplfig04_e_n2v.tiff","success":1,"access_level":"open_access","date_created":"2023-08-03T13:34:46Z","checksum":"fd0d1ec674dabbb499fab383b7eb4d58","file_id":"13734","date_updated":"2023-08-03T13:34:46Z"},{"file_id":"13735","date_updated":"2023-08-03T13:34:46Z","checksum":"86de309d9cd434afbe371da283d8adb6","date_created":"2023-08-03T13:34:46Z","access_level":"open_access","file_name":"Supplfig04_e.tif","success":1,"file_size":63974204,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl"},{"checksum":"ada99b2b5900cb198d1de3cc9e37ebd1","file_id":"13736","date_updated":"2023-08-03T13:35:37Z","access_level":"open_access","date_created":"2023-08-03T13:35:37Z","file_name":"Supplfig05_a_xy_1.png","success":1,"creator":"jdanzl","file_size":8403,"content_type":"image/png","relation":"main_file"},{"file_size":10087,"content_type":"image/png","relation":"main_file","creator":"jdanzl","file_name":"Supplfig05_a_xy_2.png","success":1,"date_created":"2023-08-03T13:35:37Z","access_level":"open_access","file_id":"13737","date_updated":"2023-08-03T13:35:37Z","checksum":"f2e382925a972b98560614016ae3bc55"},{"access_level":"open_access","date_created":"2023-08-03T13:35:37Z","checksum":"ef22b640f33d677add1a7b5153f95420","file_id":"13738","date_updated":"2023-08-03T13:35:37Z","creator":"jdanzl","file_size":24561,"content_type":"image/png","relation":"main_file","file_name":"Supplfig05_a_xy_3.png","success":1},{"checksum":"b854cc4a5224b8a6f5d59c74f5740ccd","file_id":"13739","date_updated":"2023-08-03T13:35:37Z","access_level":"open_access","date_created":"2023-08-03T13:35:37Z","file_name":"Supplfig05_a_xy_4.png","success":1,"creator":"jdanzl","file_size":8353,"content_type":"image/png","relation":"main_file"},{"file_size":8337,"content_type":"image/png","relation":"main_file","creator":"jdanzl","file_name":"Supplfig05_a_xy_5.png","success":1,"date_created":"2023-08-03T13:35:37Z","access_level":"open_access","file_id":"13740","date_updated":"2023-08-03T13:35:37Z","checksum":"446b0362c5ea6e2a5f0e1f8359422358"},{"creator":"jdanzl","content_type":"image/png","relation":"main_file","file_size":7399,"success":1,"file_name":"Supplfig05_a_xz_1.png","access_level":"open_access","date_created":"2023-08-03T13:35:37Z","checksum":"5585f7b62fa09ed5a63090b679383601","date_updated":"2023-08-03T13:35:37Z","file_id":"13741"},{"access_level":"open_access","date_created":"2023-08-03T13:35:37Z","checksum":"190d88bcacbd11d41f4a0e2bbd140862","file_id":"13742","date_updated":"2023-08-03T13:35:37Z","creator":"jdanzl","file_size":10887,"relation":"main_file","content_type":"image/png","file_name":"Supplfig05_a_xz_2.png","success":1},{"access_level":"open_access","date_created":"2023-08-03T13:35:37Z","checksum":"0c89010651862f21032a303e159b7a1c","date_updated":"2023-08-03T13:35:37Z","file_id":"13743","creator":"jdanzl","content_type":"image/png","relation":"main_file","file_size":21654,"success":1,"file_name":"Supplfig05_a_xz_3.png"},{"checksum":"94e61e553aa2280721acce7d407ff24a","file_id":"13744","date_updated":"2023-08-03T13:35:37Z","access_level":"open_access","date_created":"2023-08-03T13:35:37Z","file_name":"Supplfig05_a_xz_4.png","success":1,"creator":"jdanzl","file_size":7248,"content_type":"image/png","relation":"main_file"},{"content_type":"image/png","relation":"main_file","file_size":7214,"creator":"jdanzl","success":1,"file_name":"Supplfig05_a_xz_5.png","date_created":"2023-08-03T13:35:37Z","access_level":"open_access","date_updated":"2023-08-03T13:35:37Z","file_id":"13745","checksum":"ef9157274686ff5d7056f5a519d413dd"},{"creator":"jdanzl","file_size":8544,"content_type":"image/png","relation":"main_file","file_name":"Supplfig05_b_xy_1.png","success":1,"access_level":"open_access","date_created":"2023-08-03T13:35:37Z","checksum":"4b389473cbf769e6e34670dae2224af1","file_id":"13746","date_updated":"2023-08-03T13:35:37Z"},{"access_level":"open_access","date_created":"2023-08-03T13:35:37Z","checksum":"b05aa1e201758a5d55e35d719452fafc","date_updated":"2023-08-03T13:35:37Z","file_id":"13747","creator":"jdanzl","content_type":"image/png","relation":"main_file","file_size":11135,"success":1,"file_name":"Supplfig05_b_xy_2.png"},{"file_id":"13748","date_updated":"2023-08-03T13:35:37Z","checksum":"890f3516930251a367066c049141d6bb","date_created":"2023-08-03T13:35:37Z","access_level":"open_access","file_name":"Supplfig05_b_xy_3.png","success":1,"file_size":26762,"relation":"main_file","content_type":"image/png","creator":"jdanzl"},{"file_name":"Supplfig05_b_xy_4.png","success":1,"creator":"jdanzl","file_size":8438,"content_type":"image/png","relation":"main_file","checksum":"36345c1d582758ff463072fd04578c0c","file_id":"13749","date_updated":"2023-08-03T13:35:37Z","access_level":"open_access","date_created":"2023-08-03T13:35:37Z"},{"date_updated":"2023-08-03T13:35:37Z","file_id":"13750","checksum":"c9499ad8914c405a07fc4469b19e8987","date_created":"2023-08-03T13:35:37Z","access_level":"open_access","success":1,"file_name":"Supplfig05_b_xy_5.png","relation":"main_file","content_type":"image/png","file_size":8434,"creator":"jdanzl"},{"file_name":"Supplfig05_b_xz_1.png","success":1,"creator":"jdanzl","file_size":8269,"content_type":"image/png","relation":"main_file","checksum":"7e7a7c900ce1fc3187ab648f207f9425","file_id":"13751","date_updated":"2023-08-03T13:35:37Z","access_level":"open_access","date_created":"2023-08-03T13:35:37Z"},{"checksum":"b645d1e2422ef3f05344ccd3f955468d","file_id":"13752","date_updated":"2023-08-03T13:35:37Z","access_level":"open_access","date_created":"2023-08-03T13:35:37Z","file_name":"Supplfig05_b_xz_2.png","success":1,"creator":"jdanzl","file_size":12762,"relation":"main_file","content_type":"image/png"},{"file_name":"Supplfig05_b_xz_3.png","success":1,"file_size":24819,"relation":"main_file","content_type":"image/png","creator":"jdanzl","file_id":"13753","date_updated":"2023-08-03T13:35:37Z","checksum":"ef0b83b49cf47ca7d8ffe3bdc609fe38","date_created":"2023-08-03T13:35:37Z","access_level":"open_access"},{"file_name":"Supplfig05_b_xz_4.png","success":1,"creator":"jdanzl","file_size":8126,"relation":"main_file","content_type":"image/png","checksum":"68719d4d464a2ffc198cb6ad070c4579","file_id":"13754","date_updated":"2023-08-03T13:35:37Z","access_level":"open_access","date_created":"2023-08-03T13:35:37Z"},{"date_updated":"2023-08-03T13:35:38Z","file_id":"13755","checksum":"18370f47177ef37a477c6fd7bda3fea0","date_created":"2023-08-03T13:35:38Z","access_level":"open_access","success":1,"file_name":"Supplfig05_b_xz_5.png","relation":"main_file","content_type":"image/png","file_size":8114,"creator":"jdanzl"},{"date_created":"2023-08-03T13:35:38Z","access_level":"open_access","file_id":"13756","date_updated":"2023-08-03T13:35:38Z","checksum":"013d4bd9704ace490810b9ef21b12454","file_size":8273,"content_type":"image/png","relation":"main_file","creator":"jdanzl","file_name":"Supplfig05_c_xy_1.png","success":1},{"access_level":"open_access","date_created":"2023-08-03T13:35:38Z","checksum":"bb431f827386e79c54452d6284f501b9","date_updated":"2023-08-03T13:35:38Z","file_id":"13757","creator":"jdanzl","content_type":"image/png","relation":"main_file","file_size":12639,"success":1,"file_name":"Supplfig05_c_xy_2.png"},{"success":1,"file_name":"Supplfig05_c_xy_3.png","creator":"jdanzl","relation":"main_file","content_type":"image/png","file_size":25288,"checksum":"4cd54f58c5afff88832a508655f913bb","date_updated":"2023-08-03T13:35:38Z","file_id":"13758","access_level":"open_access","date_created":"2023-08-03T13:35:38Z"},{"access_level":"open_access","date_created":"2023-08-03T13:35:38Z","checksum":"07f86f98f6a65eac8608cec8c567309e","date_updated":"2023-08-03T13:35:38Z","file_id":"13759","creator":"jdanzl","content_type":"image/png","relation":"main_file","file_size":6786,"success":1,"file_name":"Supplfig05_c_xz_1.png"},{"access_level":"open_access","date_created":"2023-08-03T13:35:38Z","checksum":"492f699b71761214272b17432e3a54fa","date_updated":"2023-08-03T13:35:38Z","file_id":"13760","creator":"jdanzl","relation":"main_file","content_type":"image/png","file_size":9781,"success":1,"file_name":"Supplfig05_c_xz_2.png"},{"file_name":"Supplfig05_c_xz_3.png","success":1,"file_size":20728,"content_type":"image/png","relation":"main_file","creator":"jdanzl","file_id":"13761","date_updated":"2023-08-03T13:35:38Z","checksum":"af617da5638db849bea6ff51a08227c6","date_created":"2023-08-03T13:35:38Z","access_level":"open_access"},{"file_name":"Supplfig05_d_xy_1.png","success":1,"file_size":8428,"content_type":"image/png","relation":"main_file","creator":"jdanzl","file_id":"13762","date_updated":"2023-08-03T13:35:38Z","checksum":"5e5d5694af4a22297046d697a01fa4d6","date_created":"2023-08-03T13:35:38Z","access_level":"open_access"},{"date_created":"2023-08-03T13:35:38Z","access_level":"open_access","file_id":"13763","date_updated":"2023-08-03T13:35:38Z","checksum":"38bf7b75f3dedd0a3722faae05aa0233","file_size":10813,"content_type":"image/png","relation":"main_file","creator":"jdanzl","file_name":"Supplfig05_d_xy_2.png","success":1},{"success":1,"file_name":"Supplfig05_d_xy_3.png","content_type":"image/png","relation":"main_file","file_size":26552,"creator":"jdanzl","date_updated":"2023-08-03T13:35:38Z","file_id":"13764","checksum":"e4ea966553f158ec2cfbdc696780390e","date_created":"2023-08-03T13:35:38Z","access_level":"open_access"},{"file_name":"Supplfig05_d_xz_1.png","success":1,"file_size":7876,"content_type":"image/png","relation":"main_file","creator":"jdanzl","file_id":"13765","date_updated":"2023-08-03T13:35:38Z","checksum":"356f424d9f5f608e9aea962bb63672f1","date_created":"2023-08-03T13:35:38Z","access_level":"open_access"},{"file_size":11634,"relation":"main_file","content_type":"image/png","creator":"jdanzl","file_name":"Supplfig05_d_xz_2.png","success":1,"date_created":"2023-08-03T13:35:38Z","access_level":"open_access","file_id":"13766","date_updated":"2023-08-03T13:35:38Z","checksum":"05068476beee0f394e28b98209686394"},{"file_size":25006,"content_type":"image/png","relation":"main_file","creator":"jdanzl","file_name":"Supplfig05_d_xz_3.png","success":1,"date_created":"2023-08-03T13:35:38Z","access_level":"open_access","file_id":"13767","date_updated":"2023-08-03T13:35:38Z","checksum":"78b3a0d93df22383a384a2f3acc80401"},{"success":1,"file_name":"Supplfig05_e_xy_1.png","creator":"jdanzl","content_type":"image/png","relation":"main_file","file_size":7895,"checksum":"f5ee6a817f11a42c9db82ad6325f58b9","date_updated":"2023-08-03T13:35:38Z","file_id":"13768","access_level":"open_access","date_created":"2023-08-03T13:35:38Z"},{"creator":"jdanzl","file_size":6180,"relation":"main_file","content_type":"image/png","file_name":"Supplfig05_e_xy_2.png","success":1,"access_level":"open_access","date_created":"2023-08-03T13:35:38Z","checksum":"019ca89e892d6e19589609bc99eab331","file_id":"13769","date_updated":"2023-08-03T13:35:38Z"},{"date_updated":"2023-08-03T13:35:38Z","file_id":"13770","checksum":"0e1a5cbbd70dc245edaecaca70d974cb","date_created":"2023-08-03T13:35:38Z","access_level":"open_access","success":1,"file_name":"Supplfig05_e_xy_3.png","relation":"main_file","content_type":"image/png","file_size":18326,"creator":"jdanzl"},{"date_updated":"2023-08-03T13:35:38Z","file_id":"13771","checksum":"4d4d64a5bb7b096aaf8130ece4512bc2","date_created":"2023-08-03T13:35:38Z","access_level":"open_access","success":1,"file_name":"Supplfig05_e_xz_1.png","content_type":"image/png","relation":"main_file","file_size":6295,"creator":"jdanzl"},{"date_created":"2023-08-03T13:35:38Z","access_level":"open_access","file_id":"13772","date_updated":"2023-08-03T13:35:38Z","checksum":"607302aec5ec2a14a271dbffd3465261","file_size":4335,"relation":"main_file","content_type":"image/png","creator":"jdanzl","file_name":"Supplfig05_e_xz_2.png","success":1},{"file_size":23243,"content_type":"image/png","relation":"main_file","creator":"jdanzl","file_name":"Supplfig05_e_xz_3.png","success":1,"date_created":"2023-08-03T13:35:38Z","access_level":"open_access","file_id":"13773","date_updated":"2023-08-03T13:35:38Z","checksum":"a673e17ead1fd2e776b9a5f560a289ab"},{"date_updated":"2023-08-03T13:46:13Z","file_id":"13774","checksum":"4349e511fc86a8bddb790a7be7175c75","date_created":"2023-08-03T13:46:13Z","access_level":"open_access","success":1,"file_name":"Supplfig06_a_1.tif","relation":"main_file","content_type":"image/tiff","file_size":4002153,"creator":"jdanzl"},{"creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":2252137,"success":1,"file_name":"Supplfig06_a_2.tif","access_level":"open_access","date_created":"2023-08-03T13:46:13Z","checksum":"cd12977e4c1a472d80193be01f69a895","date_updated":"2023-08-03T13:46:13Z","file_id":"13775"},{"file_id":"13776","date_updated":"2023-08-03T13:46:13Z","checksum":"575e32b8c25ce658e20f0c86a5f1b9b7","date_created":"2023-08-03T13:46:13Z","access_level":"open_access","file_name":"Supplfig06_a_3.tif","success":1,"file_size":2252137,"content_type":"image/tiff","relation":"main_file","creator":"jdanzl"},{"creator":"jdanzl","file_size":78329,"relation":"main_file","content_type":"image/tiff","file_name":"Supplfig06_a_i.tif","success":1,"access_level":"open_access","date_created":"2023-08-03T13:46:13Z","checksum":"b06f775e0210e7a16cb4b58aed32118c","file_id":"13777","date_updated":"2023-08-03T13:46:13Z"},{"file_name":"Supplfig06_a_ii.tif","success":1,"file_size":78329,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl","file_id":"13778","date_updated":"2023-08-03T13:46:13Z","checksum":"56854c1a05c721c36877fe7c7454b7d7","date_created":"2023-08-03T13:46:13Z","access_level":"open_access"},{"file_name":"Supplfig06_a_iii.tif","success":1,"file_size":78329,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl","file_id":"13779","date_updated":"2023-08-03T13:46:13Z","checksum":"9aff42ea5138931fedb5a00643ae7a22","date_created":"2023-08-03T13:46:13Z","access_level":"open_access"},{"success":1,"file_name":"Supplfig06_d_1.tif","creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":501149,"checksum":"9474b7d00d4dee46659afedef75dc095","date_updated":"2023-08-03T13:46:13Z","file_id":"13780","access_level":"open_access","date_created":"2023-08-03T13:46:13Z"},{"relation":"main_file","content_type":"image/tiff","file_size":500153,"creator":"jdanzl","success":1,"file_name":"Supplfig06_d_2.tif","date_created":"2023-08-03T13:46:13Z","access_level":"open_access","date_updated":"2023-08-03T13:46:13Z","file_id":"13781","checksum":"53df41dd968b04b4657cd2b91870d636"},{"creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":500153,"success":1,"file_name":"Supplfig06_d_3.tif","access_level":"open_access","date_created":"2023-08-03T13:46:14Z","checksum":"1a39b8310598dd25d97f848b682b8de0","date_updated":"2023-08-03T13:46:14Z","file_id":"13782"},{"checksum":"6d9b496be6aecedbb2cb4cbf68f02872","file_id":"13783","date_updated":"2023-08-03T13:46:14Z","access_level":"open_access","date_created":"2023-08-03T13:46:14Z","file_name":"Supplfig06_d_i.tif","success":1,"creator":"jdanzl","file_size":78329,"relation":"main_file","content_type":"image/tiff"},{"file_size":78329,"content_type":"image/tiff","relation":"main_file","creator":"jdanzl","file_name":"Supplfig06_d_ii.tif","success":1,"date_created":"2023-08-03T13:46:14Z","access_level":"open_access","file_id":"13784","date_updated":"2023-08-03T13:46:14Z","checksum":"bc034b1636c2db8b9d9e7bd131b9780a"},{"date_created":"2023-08-03T13:46:14Z","access_level":"open_access","file_id":"13785","date_updated":"2023-08-03T13:46:14Z","checksum":"54f6e55e30a2aeea6c82eb6dbd376240","file_size":78329,"content_type":"image/tiff","relation":"main_file","creator":"jdanzl","file_name":"Supplfig06_d_iii.tif","success":1},{"access_level":"open_access","date_created":"2023-08-03T13:46:14Z","checksum":"446da3cc16897277890f23f966c2bf52","date_updated":"2023-08-03T13:46:14Z","file_id":"13786","creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":960011,"success":1,"file_name":"Supplfig06_g_1.tif"},{"checksum":"71192d0ec592a33195f2386a5f95aa86","date_updated":"2023-08-03T13:46:14Z","file_id":"13787","access_level":"open_access","date_created":"2023-08-03T13:46:14Z","success":1,"file_name":"Supplfig06_g_2.tif","creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":960011},{"success":1,"file_name":"Supplfig06_g_3.tif","content_type":"image/tiff","relation":"main_file","file_size":960041,"creator":"jdanzl","date_updated":"2023-08-03T13:46:14Z","file_id":"13788","checksum":"246782dbe8ff525aea360efc72e65f07","date_created":"2023-08-03T13:46:14Z","access_level":"open_access"},{"file_size":960041,"content_type":"image/tiff","relation":"main_file","creator":"jdanzl","file_name":"Supplfig06_g_4.tif","success":1,"date_created":"2023-08-03T13:46:14Z","access_level":"open_access","file_id":"13789","date_updated":"2023-08-03T13:46:14Z","checksum":"d49b12fec5508aa8d72587757cccc0a4"},{"relation":"main_file","content_type":"image/tiff","file_size":963241,"creator":"jdanzl","success":1,"file_name":"Supplfig06_g_5.tif","date_created":"2023-08-03T13:46:14Z","access_level":"open_access","date_updated":"2023-08-03T13:46:14Z","file_id":"13790","checksum":"27b11b1d4fc784a49ffd7660624d00b6"},{"checksum":"9ce944049c1b88fadbf43609385f9e2f","file_id":"13791","date_updated":"2023-08-03T13:51:38Z","access_level":"open_access","date_created":"2023-08-03T13:51:38Z","file_name":"Suppl08_a_1.tiff","success":1,"creator":"jdanzl","file_size":19286002,"relation":"main_file","content_type":"image/tiff"},{"file_name":"Suppl08_a_2.tiff","success":1,"creator":"jdanzl","file_size":19286002,"content_type":"image/tiff","relation":"main_file","checksum":"06265515f06688843a415362f7b2c485","file_id":"13792","date_updated":"2023-08-03T13:51:38Z","access_level":"open_access","date_created":"2023-08-03T13:51:38Z"},{"access_level":"open_access","date_created":"2023-08-03T13:51:38Z","checksum":"50f0969c6c2c1209ff776bec4861030a","file_id":"13793","date_updated":"2023-08-03T13:51:38Z","creator":"jdanzl","file_size":617552,"relation":"main_file","content_type":"image/tiff","file_name":"Suppl08_a_3.tif","success":1},{"checksum":"b90965d20133fea73c99050c32eb7695","file_id":"13794","date_updated":"2023-08-03T13:51:38Z","access_level":"open_access","date_created":"2023-08-03T13:51:38Z","file_name":"Suppl08_a_4.tif","success":1,"creator":"jdanzl","file_size":617552,"relation":"main_file","content_type":"image/tiff"},{"file_id":"13795","date_updated":"2023-08-03T13:51:40Z","checksum":"568a1e0efaa62527b51e66a7aabfd05c","date_created":"2023-08-03T13:51:40Z","access_level":"open_access","file_name":"Suppl08_a_coCATS_N2V.tif","success":1,"file_size":19287339,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl"},{"creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":19286002,"success":1,"file_name":"Suppl08_c_1_4_predictedBASSOON.tiff","access_level":"open_access","date_created":"2023-08-03T13:51:40Z","checksum":"06265515f06688843a415362f7b2c485","date_updated":"2023-08-03T13:51:40Z","file_id":"13796"},{"creator":"jdanzl","file_size":19287339,"relation":"main_file","content_type":"image/tiff","file_name":"Suppl08_c_1_coCATS.tif","success":1,"access_level":"open_access","date_created":"2023-08-03T13:51:43Z","checksum":"568a1e0efaa62527b51e66a7aabfd05c","file_id":"13797","date_updated":"2023-08-03T13:51:43Z"},{"file_id":"13798","date_updated":"2023-08-03T15:36:47Z","checksum":"feae08c38b1d9c540b697977cd7f2666","date_created":"2023-08-03T15:36:47Z","access_level":"open_access","file_name":"Suppl08_c_2_coCATS.tif","success":1,"file_size":19287340,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl"},{"date_updated":"2023-08-03T13:51:44Z","file_id":"13799","checksum":"af2c1199fdcaecfec76189dae86cc12e","date_created":"2023-08-03T13:51:44Z","access_level":"open_access","success":1,"file_name":"Suppl08_c_2-3_predictedBASSOON.tiff","relation":"main_file","content_type":"image/tiff","file_size":19286002,"creator":"jdanzl"},{"creator":"jdanzl","relation":"main_file","content_type":"application/octet-stream","file_size":156,"success":1,"file_name":"Suppl08_c_3-6_roi.roi","access_level":"open_access","date_created":"2023-08-03T13:51:43Z","checksum":"1f92db47605959d0db01f612f7c9c034","date_updated":"2023-08-03T13:51:43Z","file_id":"13800"},{"file_name":"Suppl08_e_1_3.tiff","success":1,"creator":"jdanzl","file_size":19286002,"content_type":"image/tiff","relation":"main_file","checksum":"4fc4f8803adcfacd53a41b91f4699dc5","file_id":"13801","date_updated":"2023-08-03T13:51:45Z","access_level":"open_access","date_created":"2023-08-03T13:51:45Z"},{"success":1,"file_name":"Suppl08_e_1-2.tiff","creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":19286002,"checksum":"459551aae114daa4f5ca5d06fcd6b203","date_updated":"2023-08-03T13:51:45Z","file_id":"13802","access_level":"open_access","date_created":"2023-08-03T13:51:45Z"},{"creator":"jdanzl","content_type":"application/octet-stream","relation":"main_file","file_size":152,"success":1,"file_name":"Suppl08_e_2-5_roi.roi","access_level":"open_access","date_created":"2023-08-03T13:51:45Z","checksum":"032cbace7ec436f7b62478c0ffd35e5e","date_updated":"2023-08-03T13:51:45Z","file_id":"13803"},{"file_name":"Suppl08_e_4-5_coCATS.tiff","success":1,"creator":"jdanzl","file_size":19286002,"content_type":"image/tiff","relation":"main_file","checksum":"7379168be281ef618b8cecd8efd31ed5","file_id":"13804","date_updated":"2023-08-03T13:51:46Z","access_level":"open_access","date_created":"2023-08-03T13:51:46Z"},{"date_updated":"2023-08-03T13:51:46Z","file_id":"13805","checksum":"5c4c092f608f4c10bb422c9e08b5955b","date_created":"2023-08-03T13:51:46Z","access_level":"open_access","success":1,"file_name":"Suppl08_e_4.tif","content_type":"image/tiff","relation":"main_file","file_size":617552,"creator":"jdanzl"},{"date_created":"2023-08-03T13:51:46Z","access_level":"open_access","file_id":"13806","date_updated":"2023-08-03T13:51:46Z","checksum":"903e31be124f6e4ec1c2e7ab7db80e6b","file_size":617552,"content_type":"image/tiff","relation":"main_file","creator":"jdanzl","file_name":"Suppl08_e_5.tif","success":1},{"success":1,"file_name":"Suppl08_e_6_8.tiff","creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":19286002,"checksum":"173d956392d67b0a7e544629b51881e4","date_updated":"2023-08-03T13:51:48Z","file_id":"13807","access_level":"open_access","date_created":"2023-08-03T13:51:48Z"},{"relation":"main_file","content_type":"image/tiff","file_size":19286002,"creator":"jdanzl","success":1,"file_name":"Suppl08_e_6-7.tiff","date_created":"2023-08-03T13:51:48Z","access_level":"open_access","date_updated":"2023-08-03T13:51:48Z","file_id":"13808","checksum":"2a4cea867a253a6af7e9b0d2e100de14"},{"file_size":174,"content_type":"application/octet-stream","relation":"main_file","creator":"jdanzl","file_name":"Suppl08_e_7-10_roi.roi","success":1,"date_created":"2023-08-03T13:51:48Z","access_level":"open_access","file_id":"13809","date_updated":"2023-08-03T13:51:48Z","checksum":"6b40f99fddfa26f0a9713000769741b7"},{"file_name":"Suppl08_e_9-10_coCATS.tiff","success":1,"file_size":19286002,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl","file_id":"13810","date_updated":"2023-08-03T13:51:49Z","checksum":"82f7013959df99c27366886de9b38ba2","date_created":"2023-08-03T13:51:49Z","access_level":"open_access"},{"access_level":"open_access","date_created":"2023-08-03T13:51:48Z","checksum":"50f0969c6c2c1209ff776bec4861030a","date_updated":"2023-08-03T13:51:48Z","file_id":"13811","creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":617552,"success":1,"file_name":"Suppl08_e_9.tif"},{"checksum":"569f599d7f2be49d4dcf7cb385f428c4","file_id":"13812","date_updated":"2023-08-03T13:51:49Z","access_level":"open_access","date_created":"2023-08-03T13:51:49Z","file_name":"Suppl08_e_10.tif","success":1,"creator":"jdanzl","file_size":617552,"relation":"main_file","content_type":"image/tiff"},{"file_name":"Suppl08_g_1.tif","success":1,"file_size":617552,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl","file_id":"13813","date_updated":"2023-08-03T13:51:49Z","checksum":"50f0969c6c2c1209ff776bec4861030a","date_created":"2023-08-03T13:51:49Z","access_level":"open_access"},{"date_updated":"2023-08-03T13:51:49Z","file_id":"13814","checksum":"62797fc860176197142a47e831eaf1f6","date_created":"2023-08-03T13:51:49Z","access_level":"open_access","success":1,"file_name":"Suppl08_g_2.tif","content_type":"image/tiff","relation":"main_file","file_size":617552,"creator":"jdanzl"},{"content_type":"image/tiff","relation":"main_file","file_size":617552,"creator":"jdanzl","success":1,"file_name":"Suppl08_g_3.tif","date_created":"2023-08-03T13:51:49Z","access_level":"open_access","date_updated":"2023-08-03T13:51:49Z","file_id":"13815","checksum":"b90965d20133fea73c99050c32eb7695"},{"date_created":"2023-08-03T13:51:49Z","access_level":"open_access","file_id":"13816","date_updated":"2023-08-03T13:51:49Z","checksum":"a96dc9f38c6a1d3c959771a649a42748","file_size":160,"relation":"main_file","content_type":"application/octet-stream","creator":"jdanzl","file_name":"Suppl08_i_add_roi.roi","success":1},{"creator":"jdanzl","relation":"main_file","content_type":"application/octet-stream","file_size":160,"success":1,"file_name":"Suppl08_i_shape_roi.roi","access_level":"open_access","date_created":"2023-08-03T13:51:50Z","checksum":"f2135207ebbc4bdc014763ba852a5cf0","date_updated":"2023-08-03T13:51:50Z","file_id":"13817"},{"success":1,"file_name":"Suppl08_i_split_roi.roi","content_type":"application/octet-stream","relation":"main_file","file_size":158,"creator":"jdanzl","date_updated":"2023-08-03T13:51:50Z","file_id":"13818","checksum":"f6f31fe2d87cf7208387f98ce1e37b04","date_created":"2023-08-03T13:51:50Z","access_level":"open_access"},{"checksum":"71590db0ad391e0c5baf03024981b1d9","file_id":"13819","date_updated":"2023-08-03T13:51:51Z","access_level":"open_access","date_created":"2023-08-03T13:51:51Z","file_name":"Suppl08_i_1.tiff","success":1,"creator":"jdanzl","file_size":19286002,"relation":"main_file","content_type":"image/tiff"},{"access_level":"open_access","date_created":"2023-08-03T13:51:51Z","checksum":"9ce944049c1b88fadbf43609385f9e2f","file_id":"13820","date_updated":"2023-08-03T13:51:51Z","creator":"jdanzl","file_size":19286002,"content_type":"image/tiff","relation":"main_file","file_name":"Suppl08_i_2.tiff","success":1},{"file_name":"Suppl08_i_3.tiff","success":1,"file_size":19286002,"content_type":"image/tiff","relation":"main_file","creator":"jdanzl","file_id":"13821","date_updated":"2023-08-03T13:51:52Z","checksum":"06265515f06688843a415362f7b2c485","date_created":"2023-08-03T13:51:52Z","access_level":"open_access"},{"creator":"jdanzl","file_size":19284766,"content_type":"image/tiff","relation":"main_file","file_name":"Suppl08_i_4.tif","success":1,"access_level":"open_access","date_created":"2023-08-03T13:51:52Z","checksum":"16c628c160fb81e40df0ad1eb134e92b","file_id":"13822","date_updated":"2023-08-03T13:51:52Z"},{"creator":"jdanzl","file_size":9649818,"content_type":"image/tiff","relation":"main_file","file_name":"Suppl08_i_5.tif","success":1,"access_level":"open_access","date_created":"2023-08-03T13:51:53Z","checksum":"b06e62c3a9834a40002d7aad3eaacc3f","file_id":"13823","date_updated":"2023-08-03T13:51:53Z"},{"access_level":"open_access","date_created":"2023-08-03T13:51:53Z","checksum":"41ef98c7f01755b43cf6e77fff287732","date_updated":"2023-08-03T13:51:53Z","file_id":"13824","creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":19284766,"success":1,"file_name":"Suppl08_i_6.tif"},{"relation":"main_file","content_type":"image/tiff","file_size":19287340,"creator":"jdanzl","success":1,"file_name":"Suppl08_c_2_coCATS.tif","date_created":"2023-08-03T13:53:29Z","access_level":"open_access","date_updated":"2023-08-03T13:53:29Z","file_id":"13825","checksum":"feae08c38b1d9c540b697977cd7f2666"},{"date_updated":"2023-08-03T13:58:08Z","file_id":"13826","checksum":"c01509a1c657fbbbfdbbd484c298b6e3","date_created":"2023-08-03T13:58:08Z","access_level":"open_access","success":1,"file_name":"Supplfig09_b_1_n2v.tiff","content_type":"image/tiff","relation":"main_file","file_size":179788642,"creator":"jdanzl"},{"date_created":"2023-08-03T13:58:20Z","access_level":"open_access","date_updated":"2023-08-03T13:58:20Z","file_id":"13827","checksum":"64a7f270274ff3480b18d690087016e2","relation":"main_file","content_type":"image/tiff","file_size":742420240,"creator":"jdanzl","success":1,"file_name":"Supplfig09_b_2_n2v.tif"},{"creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":478900363,"success":1,"file_name":"Supplfig09_b_3_n2v.tif","access_level":"open_access","date_created":"2023-08-03T13:58:20Z","checksum":"b34a6beaaf13698f421df961e009efeb","date_updated":"2023-08-03T13:58:20Z","file_id":"13828"},{"creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":191873159,"success":1,"file_name":"Supplfig09_b_4_n2v.tif","access_level":"open_access","date_created":"2023-08-03T13:58:25Z","checksum":"ec04501f234b5e4fd91f9eac0e585395","date_updated":"2023-08-03T13:58:25Z","file_id":"13829"},{"success":1,"file_name":"Supplfig11_i.tif","creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":55286,"checksum":"3a1b313298da35c97550abf0d245b4a7","date_updated":"2023-08-03T13:59:27Z","file_id":"13830","access_level":"open_access","date_created":"2023-08-03T13:59:27Z"},{"file_size":55286,"content_type":"image/tiff","relation":"main_file","creator":"jdanzl","file_name":"Supplfig11_ii.tif","success":1,"date_created":"2023-08-03T13:59:27Z","access_level":"open_access","file_id":"13831","date_updated":"2023-08-03T13:59:27Z","checksum":"9cf00c0252f0a2efe1994c83c224389a"},{"checksum":"9a6e83acdf6d70bb9a6e76fa7d41a372","date_updated":"2023-08-03T13:59:27Z","file_id":"13832","access_level":"open_access","date_created":"2023-08-03T13:59:27Z","success":1,"file_name":"Supplfig11_iii.tif","creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":55257},{"file_id":"13833","date_updated":"2023-08-03T13:59:32Z","checksum":"709fdd13b7e090846a4d8edd2425f675","date_created":"2023-08-03T13:59:32Z","access_level":"open_access","file_name":"Supplfig11_n2v+prediction.tif","success":1,"file_size":309457551,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl"},{"date_updated":"2023-08-03T14:14:32Z","file_id":"13834","checksum":"e60ea3e6bb1bf380460d0d8ccdbd1f07","date_created":"2023-08-03T14:14:32Z","access_level":"open_access","success":1,"file_name":"Supplfig12_a_01_n2v.tif","content_type":"image/tiff","relation":"main_file","file_size":119323629,"creator":"jdanzl"},{"access_level":"open_access","date_created":"2023-08-03T14:14:29Z","checksum":"2a4e1771e2f06383894361ba2a8155b4","file_id":"13835","date_updated":"2023-08-03T14:14:29Z","creator":"jdanzl","file_size":59680727,"relation":"main_file","content_type":"image/tiff","file_name":"Supplfig12_a_01.tif","success":1},{"creator":"jdanzl","file_size":106696749,"content_type":"image/tiff","relation":"main_file","file_name":"Supplfig12_a_02_n2v.tif","success":1,"access_level":"open_access","date_created":"2023-08-03T14:14:34Z","checksum":"993c434069b9d1bcb09bfe70628f5bbe","file_id":"13836","date_updated":"2023-08-03T14:14:34Z"},{"file_name":"Supplfig12_a_02.tif","success":1,"file_size":53367287,"content_type":"image/tiff","relation":"main_file","creator":"jdanzl","file_id":"13837","date_updated":"2023-08-03T14:14:34Z","checksum":"e3b6410d68e42004ef3857967707aeb3","date_created":"2023-08-03T14:14:34Z","access_level":"open_access"},{"access_level":"open_access","date_created":"2023-08-03T14:14:41Z","checksum":"0156e99806fe1539d5f48ea16c78e8c8","file_id":"13838","date_updated":"2023-08-03T14:14:41Z","creator":"jdanzl","file_size":149937979,"content_type":"image/tiff","relation":"main_file","file_name":"Supplfig12_a_03_n2v.tif","success":1},{"access_level":"open_access","date_created":"2023-08-03T14:14:39Z","checksum":"6cc1533a221526a072a0b09fb44bcf50","date_updated":"2023-08-03T14:14:39Z","file_id":"13839","creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":74987927,"success":1,"file_name":"Supplfig12_a_03.tif"},{"date_created":"2023-08-03T14:14:53Z","access_level":"open_access","file_id":"13840","date_updated":"2023-08-03T14:14:53Z","checksum":"f134834bd0d5e8c22e5a35aadf592007","file_size":305581862,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl","file_name":"Supplfig12_a_04_n2v.tif","success":1},{"checksum":"212ec76c02552fa1eef91e7bca9f07f7","file_id":"13841","date_updated":"2023-08-03T14:14:50Z","access_level":"open_access","date_created":"2023-08-03T14:14:50Z","file_name":"Supplfig12_a_04.tif","success":1,"creator":"jdanzl","file_size":152821840,"relation":"main_file","content_type":"image/tiff"},{"file_name":"Supplfig12_a_05_n2v.tif","success":1,"file_size":48082029,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl","file_id":"13842","date_updated":"2023-08-03T14:14:52Z","checksum":"7d99807d5b4313b04b4b5e0fbc08ba78","date_created":"2023-08-03T14:14:52Z","access_level":"open_access"},{"access_level":"open_access","date_created":"2023-08-03T14:14:53Z","checksum":"a6d4024a8c6fa073a532cc0c78323df7","file_id":"13843","date_updated":"2023-08-03T14:14:53Z","creator":"jdanzl","file_size":24059983,"relation":"main_file","content_type":"image/tiff","file_name":"Supplfig12_a_05.tif","success":1},{"date_updated":"2023-08-03T14:15:09Z","file_id":"13844","checksum":"1c42ad5423636478fe57495a3b506746","date_created":"2023-08-03T14:15:09Z","access_level":"open_access","success":1,"file_name":"Supplfig12_a_06_n2v.tif","relation":"main_file","content_type":"image/tiff","file_size":305581860,"creator":"jdanzl"},{"access_level":"open_access","date_created":"2023-08-03T14:15:04Z","checksum":"821f1d31c6dbe68eb2513fb7520b5272","file_id":"13845","date_updated":"2023-08-03T14:15:04Z","creator":"jdanzl","file_size":152821840,"relation":"main_file","content_type":"image/tiff","file_name":"Supplfig12_a_06.tif","success":1},{"access_level":"open_access","date_created":"2023-08-03T14:15:08Z","checksum":"86a4dc1e4948c4bc9c71e6d55b4fd104","file_id":"13846","date_updated":"2023-08-03T14:15:08Z","creator":"jdanzl","file_size":66855793,"relation":"main_file","content_type":"image/tiff","file_name":"Supplfig12_a_07_n2v.tif","success":1},{"success":1,"file_name":"Supplfig12_a_07.tif","creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":33446807,"checksum":"fd5d45b1135800124fec635c4667dafe","date_updated":"2023-08-03T14:15:09Z","file_id":"13847","access_level":"open_access","date_created":"2023-08-03T14:15:09Z"},{"file_name":"Supplfig12_a_08_n2v.tif","success":1,"file_size":231476260,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl","file_id":"13848","date_updated":"2023-08-03T14:15:19Z","checksum":"5683760cc119a09f0b7de8fea98cda9c","date_created":"2023-08-03T14:15:19Z","access_level":"open_access"},{"content_type":"image/tiff","relation":"main_file","file_size":115769040,"creator":"jdanzl","success":1,"file_name":"Supplfig12_a_08.tif","date_created":"2023-08-03T14:15:15Z","access_level":"open_access","date_updated":"2023-08-03T14:15:15Z","file_id":"13849","checksum":"0c336d554bcd8524f11bd25c2188b56f"},{"content_type":"image/tiff","relation":"main_file","file_size":305581869,"creator":"jdanzl","success":1,"file_name":"Supplfig12_a_09_n2v.tif","date_created":"2023-08-03T14:15:31Z","access_level":"open_access","date_updated":"2023-08-03T14:15:31Z","file_id":"13850","checksum":"fcaea6c95c363bd864a524fca8ed8e9b"},{"creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":152821840,"success":1,"file_name":"Supplfig12_a_09.tif","access_level":"open_access","date_created":"2023-08-03T14:15:28Z","checksum":"f401f1875f072c345d106a1e419f9d13","date_updated":"2023-08-03T14:15:28Z","file_id":"13851"},{"access_level":"open_access","date_created":"2023-08-03T14:15:42Z","checksum":"7ad7d5b1ac81da1ec3d5003d00ee870a","date_updated":"2023-08-03T14:15:42Z","file_id":"13852","creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":266386255,"success":1,"file_name":"Supplfig12_a_10_n2v.tif"},{"checksum":"77b93d082debd5248348c0a1e886eab9","file_id":"13853","date_updated":"2023-08-03T14:15:40Z","access_level":"open_access","date_created":"2023-08-03T14:15:40Z","file_name":"Supplfig12_a_10.tif","success":1,"creator":"jdanzl","file_size":133239040,"relation":"main_file","content_type":"image/tiff"},{"date_updated":"2023-08-03T14:15:49Z","file_id":"13854","checksum":"5ece1d503f05133d07d5b867f666d7a8","date_created":"2023-08-03T14:15:49Z","access_level":"open_access","success":1,"file_name":"Supplfig12_a_11_n2v.tif","content_type":"image/tiff","relation":"main_file","file_size":191866269,"creator":"jdanzl"},{"access_level":"open_access","date_created":"2023-08-03T14:15:48Z","checksum":"a72ee7ab433097752ff00a63421d4b61","file_id":"13855","date_updated":"2023-08-03T14:15:48Z","creator":"jdanzl","file_size":95955040,"content_type":"image/tiff","relation":"main_file","file_name":"Supplfig12_a_11.tif","success":1},{"date_created":"2023-08-03T14:16:49Z","access_level":"open_access","date_updated":"2023-08-03T14:16:49Z","file_id":"13856","checksum":"c9c6e4692e0e7f305a43058b201b50d3","content_type":"image/tiff","relation":"main_file","file_size":55281,"creator":"jdanzl","success":1,"file_name":"Supplfig13_i.tif"},{"access_level":"open_access","date_created":"2023-08-03T14:16:49Z","checksum":"345d11dbd70123d9d2723b547cbfc4fc","date_updated":"2023-08-03T14:16:49Z","file_id":"13857","creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":55193,"success":1,"file_name":"Supplfig13_ii.tif"},{"date_created":"2023-08-03T14:16:49Z","access_level":"open_access","date_updated":"2023-08-03T14:16:49Z","file_id":"13858","checksum":"b4c74c08077cfd3e11bfd6ea9ee1c5b2","content_type":"image/tiff","relation":"main_file","file_size":539037,"creator":"jdanzl","success":1,"file_name":"Supplfig13_n2v+predict.tif"},{"file_size":854898609,"content_type":"image/tiff","relation":"main_file","creator":"jdanzl","file_name":"Supplfig14_a_n2v.tif","success":1,"date_created":"2023-08-03T14:18:10Z","access_level":"open_access","file_id":"13859","date_updated":"2023-08-03T14:18:10Z","checksum":"15f5b44257d075f9e45811304dfd5f85"},{"creator":"jdanzl","file_size":7514502,"content_type":"image/png","relation":"main_file","file_name":"Supplfig15_a_1.png","success":1,"access_level":"open_access","date_created":"2023-08-03T14:19:58Z","checksum":"a9ca03e3e5696988d06fc6ab1c103440","file_id":"13860","date_updated":"2023-08-03T14:19:58Z"},{"access_level":"open_access","date_created":"2023-08-03T14:19:58Z","checksum":"4be3451e203a0fc5d9eb14279ff68a7a","file_id":"13861","date_updated":"2023-08-03T14:19:58Z","creator":"jdanzl","file_size":1784041,"relation":"main_file","content_type":"image/png","file_name":"Supplfig15_a_2.png","success":1},{"file_name":"Supplfig15_b_1.tif","success":1,"file_size":6030878,"content_type":"image/tiff","relation":"main_file","creator":"jdanzl","file_id":"13862","date_updated":"2023-08-03T14:19:58Z","checksum":"43068c31eecddb798dbf2f51bda9b6c9","date_created":"2023-08-03T14:19:58Z","access_level":"open_access"},{"file_id":"13863","date_updated":"2023-08-03T14:19:58Z","checksum":"ded4c0797f1a9152dc3128f1bb8c32a5","date_created":"2023-08-03T14:19:58Z","access_level":"open_access","file_name":"Supplfig15_b_2.tif","success":1,"file_size":723293,"content_type":"image/tiff","relation":"main_file","creator":"jdanzl"},{"success":1,"file_name":"Supplfig15_c_i.tif","relation":"main_file","content_type":"image/tiff","file_size":723409,"creator":"jdanzl","date_updated":"2023-08-03T14:19:58Z","file_id":"13864","checksum":"705394732d94bd8463479aee83c2d36e","date_created":"2023-08-03T14:19:58Z","access_level":"open_access"},{"creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":723409,"success":1,"file_name":"Supplfig15_c_ii.tif","access_level":"open_access","date_created":"2023-08-03T14:19:58Z","checksum":"e08efe220bec6a93d583cab17754dd19","date_updated":"2023-08-03T14:19:58Z","file_id":"13865"},{"success":1,"file_name":"Supplfig15_c.tif","relation":"main_file","content_type":"image/tiff","file_size":31572914,"creator":"jdanzl","date_updated":"2023-08-03T14:19:59Z","file_id":"13866","checksum":"0e08e5d965815240b0a8195e0f899aab","date_created":"2023-08-03T14:19:59Z","access_level":"open_access"},{"file_name":"Supplfig15_d_1.tif","success":1,"file_size":6030752,"content_type":"image/tiff","relation":"main_file","creator":"jdanzl","file_id":"13867","date_updated":"2023-08-03T14:19:59Z","checksum":"594696a77d4a9be512046be5e2d602f0","date_created":"2023-08-03T14:19:59Z","access_level":"open_access"},{"checksum":"ffe6733eb77858008c4ca07b5f8c432b","file_id":"13868","date_updated":"2023-08-03T14:19:59Z","access_level":"open_access","date_created":"2023-08-03T14:19:59Z","file_name":"Supplfig15_d_2.tif","success":1,"creator":"jdanzl","file_size":8005495,"content_type":"image/tiff","relation":"main_file"},{"checksum":"663bf013cf1e5019e1e7ddd2d12fa492","file_id":"13869","date_updated":"2023-08-03T14:20:56Z","access_level":"open_access","date_created":"2023-08-03T14:20:56Z","file_name":"Supplfig16_a.tif","success":1,"creator":"jdanzl","file_size":11117709,"relation":"main_file","content_type":"image/tiff"},{"checksum":"80aee233dc2a1f26ed8cc323270cf3a9","file_id":"13870","date_updated":"2023-08-03T14:20:56Z","access_level":"open_access","date_created":"2023-08-03T14:20:56Z","file_name":"Supplfig16_b.tif","success":1,"creator":"jdanzl","file_size":113709,"content_type":"image/tiff","relation":"main_file"},{"creator":"jdanzl","file_size":113709,"relation":"main_file","content_type":"image/tiff","file_name":"Supplfig16_c.tif","success":1,"access_level":"open_access","date_created":"2023-08-03T14:20:56Z","checksum":"3b408e9345df2f16a942ad4ea61739b7","file_id":"13871","date_updated":"2023-08-03T14:20:56Z"},{"creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":6003131,"success":1,"file_name":"Supplfig17_a_1.tif","access_level":"open_access","date_created":"2023-08-03T14:25:47Z","checksum":"fbf688ef1f907149f92e9e936d02df72","date_updated":"2023-08-03T14:25:47Z","file_id":"13872"},{"success":1,"file_name":"Supplfig17_a_2.tif","content_type":"image/tiff","relation":"main_file","file_size":8004085,"creator":"jdanzl","date_updated":"2023-08-03T14:25:47Z","file_id":"13873","checksum":"9a56470f251f14b43903036fab338f76","date_created":"2023-08-03T14:25:47Z","access_level":"open_access"},{"creator":"jdanzl","file_size":6003123,"relation":"main_file","content_type":"image/tiff","file_name":"Supplfig17_a_3.tif","success":1,"access_level":"open_access","date_created":"2023-08-03T14:25:47Z","checksum":"4578c020ba5d0a6ffe2a8a1c22e52c75","file_id":"13874","date_updated":"2023-08-03T14:25:47Z"},{"file_id":"13875","date_updated":"2023-08-03T14:25:47Z","checksum":"a681d32f515da3bff8e803946f1d8b50","date_created":"2023-08-03T14:25:47Z","access_level":"open_access","file_name":"Supplfig17_a_4.tif","success":1,"file_size":6003131,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl"},{"file_name":"Supplfig17_a_5.tif","success":1,"creator":"jdanzl","file_size":6003131,"content_type":"image/tiff","relation":"main_file","checksum":"b89d366c39e25d17dc5d8ceee9145c8b","file_id":"13876","date_updated":"2023-08-03T14:25:47Z","access_level":"open_access","date_created":"2023-08-03T14:25:47Z"},{"success":1,"file_name":"Supplfig17_a_6.tif","creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":6003079,"checksum":"0b9d60f0aba79525a97c122d972f0b22","date_updated":"2023-08-03T14:25:47Z","file_id":"13877","access_level":"open_access","date_created":"2023-08-03T14:25:47Z"},{"file_size":6003115,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl","file_name":"Supplfig17_a_7.tif","success":1,"date_created":"2023-08-03T14:25:47Z","access_level":"open_access","file_id":"13878","date_updated":"2023-08-03T14:25:47Z","checksum":"169aad3cea521cc2a0ce4c3e41473e0c"},{"file_name":"Supplfig17_a_8.tif","success":1,"file_size":4002129,"content_type":"image/tiff","relation":"main_file","creator":"jdanzl","file_id":"13879","date_updated":"2023-08-03T14:25:47Z","checksum":"39a44d77d9047bfa10da8d9c9bd7b391","date_created":"2023-08-03T14:25:47Z","access_level":"open_access"},{"success":1,"file_name":"Supplfig17_b.tif","relation":"main_file","content_type":"image/tiff","file_size":8214335,"creator":"jdanzl","date_updated":"2023-08-03T14:25:48Z","file_id":"13880","checksum":"f4ecb2bd44f2cea3d71587670344ac40","date_created":"2023-08-03T14:25:48Z","access_level":"open_access"},{"file_name":"Supplfig18_a.tif","success":1,"file_size":7471479,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl","file_id":"13881","date_updated":"2023-08-03T14:27:06Z","checksum":"44a20d2621680c90227416bf32b5a566","date_created":"2023-08-03T14:27:06Z","access_level":"open_access"},{"relation":"main_file","content_type":"image/tiff","file_size":290625,"creator":"jdanzl","success":1,"file_name":"Supplfig18_b.tif","date_created":"2023-08-03T14:27:06Z","access_level":"open_access","date_updated":"2023-08-03T14:27:06Z","file_id":"13882","checksum":"5a5f7b616785f9213064e8c77f9d38c4"},{"creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":411767,"success":1,"file_name":"Supplfig18_c.tif","access_level":"open_access","date_created":"2023-08-03T14:27:07Z","checksum":"8f621722a636e808a03d85f9597ad1a2","date_updated":"2023-08-03T14:27:07Z","file_id":"13883"},{"relation":"main_file","content_type":"image/tiff","file_size":11117685,"creator":"jdanzl","success":1,"file_name":"Supplfig18_d.tif","date_created":"2023-08-03T14:27:07Z","access_level":"open_access","date_updated":"2023-08-03T14:27:07Z","file_id":"13884","checksum":"3c8356f9170fe345e2ef725cd7d1b167"},{"checksum":"5c8443e97724b54f359f7355483b890e","file_id":"13885","date_updated":"2023-08-03T14:27:10Z","access_level":"open_access","date_created":"2023-08-03T14:27:10Z","file_name":"Supplfig18_e_n2v.tif","success":1,"creator":"jdanzl","file_size":259509773,"relation":"main_file","content_type":"image/tiff"},{"file_name":"Supplfig19_n2v.tif","success":1,"creator":"jdanzl","file_size":1430939360,"relation":"main_file","content_type":"image/tiff","checksum":"413e3105c1bcd2ac2c569fa2b06253f2","file_id":"13886","date_updated":"2023-08-03T14:29:25Z","access_level":"open_access","date_created":"2023-08-03T14:29:25Z"},{"date_created":"2023-08-03T14:29:16Z","access_level":"open_access","file_id":"13887","date_updated":"2023-08-03T14:29:16Z","checksum":"a864dc5b54f469c723e86286d538bb52","file_size":715524468,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl","file_name":"Supplfig19.tif","success":1},{"checksum":"8a78ce49446d207c0bf31196b788fa93","date_updated":"2023-08-03T14:32:54Z","file_id":"13888","access_level":"open_access","date_created":"2023-08-03T14:32:54Z","success":1,"file_name":"Supplfig20_a_left.tif","creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":8004743},{"date_created":"2023-08-03T14:32:54Z","access_level":"open_access","date_updated":"2023-08-03T14:32:54Z","file_id":"13889","checksum":"48fa3abfd16a00c7a3757d6452a482ec","relation":"main_file","content_type":"image/tiff","file_size":5561081,"creator":"jdanzl","success":1,"file_name":"Supplfig20_a_right.tif"},{"file_size":8456797,"content_type":"image/tiff","relation":"main_file","creator":"jdanzl","file_name":"Supplfig20_b_1.tif","success":1,"date_created":"2023-08-03T14:32:54Z","access_level":"open_access","file_id":"13890","date_updated":"2023-08-03T14:32:54Z","checksum":"85d82cd94dd3202f04b5987bc4466573"},{"file_name":"Supplfig20_b_2.tif","success":1,"creator":"jdanzl","file_size":8456795,"relation":"main_file","content_type":"image/tiff","checksum":"e16f04d5d4d40f6ec33456d0cf02e1eb","file_id":"13891","date_updated":"2023-08-03T14:32:54Z","access_level":"open_access","date_created":"2023-08-03T14:32:54Z"},{"file_size":8456796,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl","file_name":"Supplfig20_b_3.tif","success":1,"date_created":"2023-08-03T14:32:54Z","access_level":"open_access","file_id":"13892","date_updated":"2023-08-03T14:32:54Z","checksum":"ff190edb269c28b25d27bffaeb7d25cf"},{"file_id":"13893","date_updated":"2023-08-03T14:32:54Z","checksum":"deb10b81bae62d9e20e681411fb75fcc","date_created":"2023-08-03T14:32:54Z","access_level":"open_access","file_name":"Supplfig20_b_4.tif","success":1,"file_size":8538887,"content_type":"image/tiff","relation":"main_file","creator":"jdanzl"},{"creator":"jdanzl","file_size":8538851,"relation":"main_file","content_type":"image/tiff","file_name":"Supplfig20_b_5.tif","success":1,"access_level":"open_access","date_created":"2023-08-03T14:32:54Z","checksum":"5c64bb94e76030d7a9d8624bac4b0f57","file_id":"13894","date_updated":"2023-08-03T14:32:54Z"},{"success":1,"file_name":"Supplfig20_c_1.tif","content_type":"image/tiff","relation":"main_file","file_size":11077737,"creator":"jdanzl","date_updated":"2023-08-03T14:32:54Z","file_id":"13895","checksum":"d6c20232c4bd36e213b4c456c5009644","date_created":"2023-08-03T14:32:54Z","access_level":"open_access"},{"checksum":"49c3396b8960af3f6fd8f6b078971d51","date_updated":"2023-08-03T14:32:54Z","file_id":"13896","access_level":"open_access","date_created":"2023-08-03T14:32:54Z","success":1,"file_name":"Supplfig20_c_2.tif","creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":11077737},{"date_updated":"2023-08-03T14:32:55Z","file_id":"13897","checksum":"73471528f691fb337839b84c31014da4","date_created":"2023-08-03T14:32:55Z","access_level":"open_access","success":1,"file_name":"Supplfig20_c_3.tif","relation":"main_file","content_type":"image/tiff","file_size":11077737,"creator":"jdanzl"},{"date_created":"2023-08-03T14:32:55Z","access_level":"open_access","date_updated":"2023-08-03T14:32:55Z","file_id":"13898","checksum":"47a0fd1a36d7e7907ce18007bdaaa145","content_type":"image/tiff","relation":"main_file","file_size":11077737,"creator":"jdanzl","success":1,"file_name":"Supplfig20_c_4.tif"},{"checksum":"0c74b07f13fb08a91e61669368f38c9a","date_updated":"2023-08-03T14:32:55Z","file_id":"13899","access_level":"open_access","date_created":"2023-08-03T14:32:55Z","success":1,"file_name":"Supplfig20_c_5.tif","creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":11077737},{"file_id":"13900","date_updated":"2023-08-03T14:32:55Z","checksum":"fe3b76446ec8bd0d5cdba94a128a84b8","date_created":"2023-08-03T14:32:55Z","access_level":"open_access","file_name":"Supplfig20_d_1.tif","success":1,"file_size":4002133,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl"},{"date_created":"2023-08-03T14:32:55Z","access_level":"open_access","date_updated":"2023-08-03T14:32:55Z","file_id":"13901","checksum":"0570bab9a471af3d0d1d8bf9a3cb17e7","content_type":"image/tiff","relation":"main_file","file_size":4002133,"creator":"jdanzl","success":1,"file_name":"Supplfig20_d_2.tif"},{"success":1,"file_name":"Supplfig20_d_3.tif","relation":"main_file","content_type":"image/tiff","file_size":4002199,"creator":"jdanzl","date_updated":"2023-08-03T14:32:55Z","file_id":"13902","checksum":"59f330990b5c95f98a768761a009940c","date_created":"2023-08-03T14:32:55Z","access_level":"open_access"},{"checksum":"f0da06f2a278dff4bbd1aeace11700bf","date_updated":"2023-08-03T14:32:55Z","file_id":"13903","access_level":"open_access","date_created":"2023-08-03T14:32:55Z","success":1,"file_name":"Supplfig20_d_4.tif","creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":4002133},{"access_level":"open_access","date_created":"2023-08-03T14:32:55Z","checksum":"e61d49dcc0952af3623cc26a78cc6495","file_id":"13904","date_updated":"2023-08-03T14:32:55Z","creator":"jdanzl","file_size":4002133,"content_type":"image/tiff","relation":"main_file","file_name":"Supplfig20_d_5.tif","success":1},{"file_name":"Supplfig22_b_1.tif","success":1,"creator":"jdanzl","file_size":12627576,"relation":"main_file","content_type":"image/tiff","checksum":"bcf8294487abaf1a75033f388fba06ec","file_id":"13905","date_updated":"2023-08-03T14:42:01Z","access_level":"open_access","date_created":"2023-08-03T14:42:01Z"},{"checksum":"14097feb4a73e7bb1e1694cef0115cab","date_updated":"2023-08-03T14:42:01Z","file_id":"13906","access_level":"open_access","date_created":"2023-08-03T14:42:01Z","success":1,"file_name":"Supplfig22_b_2.tif","creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":8543366},{"creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":8394010,"success":1,"file_name":"Supplfig22_b_3.tif","access_level":"open_access","date_created":"2023-08-03T14:42:04Z","checksum":"7e74b8cff2ada56d47d135a6a9e71e76","date_updated":"2023-08-03T14:42:04Z","file_id":"13907"},{"checksum":"f6e6e0f0e3854fedd201ab8ff08a7651","file_id":"13908","date_updated":"2023-08-03T15:35:39Z","access_level":"open_access","date_created":"2023-08-03T15:35:39Z","file_name":"Supplfig22_b_4.tif","success":1,"creator":"jdanzl","file_size":8682992,"relation":"main_file","content_type":"image/tiff"},{"date_updated":"2023-08-03T14:42:04Z","file_id":"13909","checksum":"6ddeb84249768b46b9c2072bf85bdc8b","date_created":"2023-08-03T14:42:04Z","access_level":"open_access","success":1,"file_name":"Supplfig22_b_5.tif","content_type":"image/tiff","relation":"main_file","file_size":8503466,"creator":"jdanzl"},{"access_level":"open_access","date_created":"2023-08-03T14:42:04Z","checksum":"40648b8a4ef375b8352f11a6772ea1a6","date_updated":"2023-08-03T14:42:04Z","file_id":"13910","creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":8543342,"success":1,"file_name":"Supplfig22_b_6.tif"},{"creator":"jdanzl","file_size":33588990,"relation":"main_file","content_type":"image/tiff","file_name":"Supplfig22_b_7.tif","success":1,"access_level":"open_access","date_created":"2023-08-03T14:42:06Z","checksum":"78f601de4d88f6ccb97861eaf4a995f8","file_id":"13911","date_updated":"2023-08-03T14:42:06Z"},{"success":1,"file_name":"Supplfig22_c.tif","creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":1453171518,"checksum":"2873c488b7c8ad4d111726025086e9ea","date_updated":"2023-08-03T14:42:41Z","file_id":"13912","access_level":"open_access","date_created":"2023-08-03T14:42:41Z"},{"date_created":"2023-08-03T14:47:43Z","access_level":"open_access","date_updated":"2023-08-03T14:47:43Z","file_id":"13913","checksum":"c1d0e5e61ac0d0ddf08704769f80ac78","relation":"main_file","content_type":"image/tiff","file_size":1325919782,"creator":"jdanzl","success":1,"file_name":"Supplfig23_a.tif"},{"file_name":"Supplfig23_b.tif","success":1,"file_size":31366288,"content_type":"image/tiff","relation":"main_file","creator":"jdanzl","file_id":"13914","date_updated":"2023-08-03T14:47:25Z","checksum":"f97632984b6a73e06cc0c8c947cdcc71","date_created":"2023-08-03T14:47:25Z","access_level":"open_access"},{"creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":31366288,"success":1,"file_name":"Supplfig23_c.tif","access_level":"open_access","date_created":"2023-08-03T14:47:26Z","checksum":"16f4c17835a79d7c06e2a97b1603baf1","date_updated":"2023-08-03T14:47:26Z","file_id":"13915"},{"creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":31366428,"success":1,"file_name":"Supplfig23_d.tif","access_level":"open_access","date_created":"2023-08-03T14:47:27Z","checksum":"00f35ca77165642ca9b3bc3608bd04c1","date_updated":"2023-08-03T14:47:27Z","file_id":"13916"},{"date_updated":"2023-08-03T14:47:27Z","file_id":"13917","checksum":"1aff850f47ae8c8f05ca48ff9faa38b1","date_created":"2023-08-03T14:47:27Z","access_level":"open_access","success":1,"file_name":"Supplfig23_e.tif","relation":"main_file","content_type":"image/tiff","file_size":31500832,"creator":"jdanzl"},{"access_level":"open_access","date_created":"2023-08-03T14:48:38Z","checksum":"f644de697d142c83461ed5ad1d72a043","file_id":"13918","date_updated":"2023-08-03T14:48:38Z","creator":"jdanzl","file_size":44179078,"content_type":"image/tiff","relation":"main_file","file_name":"Supplfig24_a_i.tif","success":1},{"file_size":44125924,"content_type":"image/tiff","relation":"main_file","creator":"jdanzl","file_name":"Supplfig24_a_ii.tif","success":1,"date_created":"2023-08-03T14:48:38Z","access_level":"open_access","file_id":"13919","date_updated":"2023-08-03T14:48:38Z","checksum":"eccb84925a6699b396d4b07efcbc7349"},{"success":1,"file_name":"Supplfig24_a.tif","creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":1061835435,"checksum":"afe7253e70ec79144e59b28c3c66703a","date_updated":"2023-08-03T14:48:54Z","file_id":"13920","access_level":"open_access","date_created":"2023-08-03T14:48:54Z"},{"success":1,"file_name":"Supplfig24_b_iii.tif","content_type":"image/tiff","relation":"main_file","file_size":3977824,"creator":"jdanzl","date_updated":"2023-08-03T14:48:39Z","file_id":"13921","checksum":"94a03b022aa84ea6e8515450a481ad82","date_created":"2023-08-03T14:48:39Z","access_level":"open_access"},{"date_created":"2023-08-03T14:48:39Z","access_level":"open_access","date_updated":"2023-08-03T14:48:39Z","file_id":"13922","checksum":"166832c173fcda88ec6e35decdf0d197","relation":"main_file","content_type":"image/tiff","file_size":3977825,"creator":"jdanzl","success":1,"file_name":"Supplfig24_b_iv.tif"},{"date_created":"2023-08-03T14:51:27Z","access_level":"open_access","file_id":"13923","date_updated":"2023-08-03T14:51:27Z","checksum":"53175073b83ab569241ae64ef7f180b3","file_size":37641738,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl","file_name":"Supplfig25_a_1.tif","success":1},{"date_created":"2023-08-03T14:51:26Z","access_level":"open_access","date_updated":"2023-08-03T14:51:26Z","file_id":"13924","checksum":"70e6a1780daeae6a5bef2c27c996c442","content_type":"image/tiff","relation":"main_file","file_size":4932897,"creator":"jdanzl","success":1,"file_name":"Supplfig25_a_2.tif"},{"creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":357433570,"success":1,"file_name":"Supplfig25_b_1.tif","access_level":"open_access","date_created":"2023-08-03T14:51:31Z","checksum":"1748266d7b8a2ae1f6292b9c265c2068","date_updated":"2023-08-03T14:51:31Z","file_id":"13925"},{"success":1,"file_name":"Supplfig25_b_2.tif","content_type":"image/tiff","relation":"main_file","file_size":27734506,"creator":"jdanzl","date_updated":"2023-08-03T14:51:27Z","file_id":"13926","checksum":"8490f44160a482ea06d858eb3bc25a11","date_created":"2023-08-03T14:51:27Z","access_level":"open_access"},{"access_level":"open_access","date_created":"2023-08-03T14:53:43Z","checksum":"e5e9c550cc934a7725dc209c79951847","date_updated":"2023-08-03T14:53:43Z","file_id":"13927","creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":35082394,"success":1,"file_name":"Suppfig26_h.tif"},{"checksum":"39ae411d1e950e78bdc12937fb36f661","file_id":"13928","date_updated":"2023-08-03T14:53:42Z","access_level":"open_access","date_created":"2023-08-03T14:53:42Z","file_name":"Supplfig26_a_1.tif","success":1,"creator":"jdanzl","file_size":16676477,"content_type":"image/tiff","relation":"main_file"},{"access_level":"open_access","date_created":"2023-08-03T14:53:42Z","checksum":"450f5d0e8091639e27eb7a7991524e88","date_updated":"2023-08-03T14:53:42Z","file_id":"13929","creator":"jdanzl","content_type":"image/tiff","relation":"main_file","file_size":170493,"success":1,"file_name":"Supplfig26_a_2.tif"},{"relation":"main_file","content_type":"image/tiff","file_size":11117737,"creator":"jdanzl","success":1,"file_name":"Supplfig26_b_1.tif","date_created":"2023-08-03T14:53:43Z","access_level":"open_access","date_updated":"2023-08-03T14:53:43Z","file_id":"13930","checksum":"8e5a71801fa1fcb7f679d1af23344662"},{"creator":"jdanzl","file_size":113753,"relation":"main_file","content_type":"image/tiff","file_name":"Supplfig26_b_2.tif","success":1,"access_level":"open_access","date_created":"2023-08-03T14:53:43Z","checksum":"519fb0326a0f0774b3edc1dafffcdd08","file_id":"13931","date_updated":"2023-08-03T14:53:43Z"},{"file_name":"Supplfig26_c.tif","success":1,"file_size":495452386,"content_type":"image/tiff","relation":"main_file","creator":"jdanzl","file_id":"13932","date_updated":"2023-08-03T14:53:52Z","checksum":"d051c7d717eeb6f2ca59d8727ea62200","date_created":"2023-08-03T14:53:52Z","access_level":"open_access"},{"file_id":"13933","date_updated":"2023-08-03T14:53:46Z","checksum":"5437b53e708572e2e9ad1432c260a04f","date_created":"2023-08-03T14:53:46Z","access_level":"open_access","file_name":"Supplfig26_d.tif","success":1,"file_size":132167170,"content_type":"image/tiff","relation":"main_file","creator":"jdanzl"},{"date_updated":"2023-08-03T14:53:46Z","file_id":"13934","checksum":"0baab38829a8587ded5e39f85068124b","date_created":"2023-08-03T14:53:46Z","access_level":"open_access","success":1,"file_name":"Supplfig26_e.tif","content_type":"image/tiff","relation":"main_file","file_size":11431214,"creator":"jdanzl"},{"access_level":"open_access","date_created":"2023-08-03T14:53:48Z","checksum":"afa57f254c9dd987d90d5e43db3fb9e6","file_id":"13935","date_updated":"2023-08-03T14:53:48Z","creator":"jdanzl","file_size":64005702,"relation":"main_file","content_type":"image/tiff","file_name":"Supplfig26_f.tif","success":1},{"success":1,"file_name":"Supplfig26_g.tif","content_type":"image/tiff","relation":"main_file","file_size":246421054,"creator":"jdanzl","date_updated":"2023-08-03T14:53:52Z","file_id":"13936","checksum":"c35ba67ab92c410cb4370f91f3dea819","date_created":"2023-08-03T14:53:52Z","access_level":"open_access"},{"checksum":"3fd6186d73cbce1be79cf14a94a7f3bd","file_id":"13937","date_updated":"2023-08-03T14:56:43Z","access_level":"open_access","date_created":"2023-08-03T14:56:43Z","file_name":"Supplfig27_a.tif","success":1,"creator":"jdanzl","file_size":166614932,"content_type":"image/tiff","relation":"main_file"},{"access_level":"open_access","date_created":"2023-08-03T14:56:42Z","checksum":"d1fc4c88e8e258e4e536a26e196d09b0","file_id":"13938","date_updated":"2023-08-03T14:56:42Z","creator":"jdanzl","file_size":143583446,"relation":"main_file","content_type":"image/tiff","file_name":"Supplfig27_b.tif","success":1},{"file_id":"13939","date_updated":"2023-08-03T14:56:47Z","checksum":"8d6985530e86f38548e89e6380d73771","date_created":"2023-08-03T14:56:47Z","access_level":"open_access","file_name":"Supplfig27_c.tif","success":1,"file_size":172528188,"relation":"main_file","content_type":"image/tiff","creator":"jdanzl"},{"date_created":"2023-08-03T14:56:46Z","access_level":"open_access","date_updated":"2023-08-03T14:56:46Z","file_id":"13940","checksum":"d0b1309d2120892ede84e04fdf0dbfbf","relation":"main_file","content_type":"image/tiff","file_size":113699256,"creator":"jdanzl","success":1,"file_name":"Supplfig27_d.tif"},{"date_created":"2023-08-03T14:56:47Z","access_level":"open_access","date_updated":"2023-08-03T14:56:47Z","file_id":"13941","checksum":"4789b5254d0415ce314fc9e39cc52121","relation":"main_file","content_type":"image/tiff","file_size":88664548,"creator":"jdanzl","success":1,"file_name":"Supplfig27_e.tif"},{"access_level":"open_access","date_created":"2023-08-03T14:56:47Z","checksum":"7ff2d06534f062b0f17dab181f6c1f4e","date_updated":"2023-08-03T14:56:47Z","file_id":"13942","creator":"jdanzl","relation":"main_file","content_type":"image/tiff","file_size":2298356,"success":1,"file_name":"Supplfig27_f.tif"},{"success":1,"file_name":"Supplfig27_g_1.tif","content_type":"image/tiff","relation":"main_file","file_size":499618,"creator":"jdanzl","date_updated":"2023-08-03T14:56:47Z","file_id":"13943","checksum":"46de66d457b0def90f753c11927536b7","date_created":"2023-08-03T14:56:47Z","access_level":"open_access"},{"creator":"jdanzl","file_size":202079026,"relation":"main_file","content_type":"image/tiff","file_name":"Supplfig27_g_2.tif","success":1,"access_level":"open_access","date_created":"2023-08-03T14:56:50Z","checksum":"9ef5fba064a73a79dbc74b5e166d08ec","file_id":"13944","date_updated":"2023-08-03T14:56:50Z"},{"creator":"jdanzl","file_size":14639269,"relation":"main_file","content_type":"image/tiff","file_name":"Supplfig27_g_3.tif","success":1,"access_level":"open_access","date_created":"2023-08-03T14:56:48Z","checksum":"bf956c2b58d3da2baa869db5206d8d49","file_id":"13945","date_updated":"2023-08-03T14:56:48Z"},{"success":1,"file_name":"model.p","relation":"main_file","content_type":"application/octet-stream","file_size":279467712,"creator":"jdanzl","date_updated":"2023-08-03T15:21:01Z","file_id":"13946","checksum":"327dca11f42d5375d7f47f4cbd5b36c0","date_created":"2023-08-03T15:21:01Z","access_level":"open_access"}],"day":"04","title":"Research data for the publication \"Imaging brain tissue architecture across millimeter to nanometer scales\"","project":[{"grant_number":"I03600","_id":"265CB4D0-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","name":"Optical control of synaptic function via adhesion molecules"},{"_id":"26AA4EF2-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","name":"Molecular Drug Targets","grant_number":"W1232-B24"},{"call_identifier":"FWF","_id":"25C5A090-B435-11E9-9278-68D0E5697425","name":"The Wittgenstein Prize","grant_number":"Z00312"},{"name":"High content imaging to decode human immune cell interactions in health and allergic disease","_id":"23889792-32DE-11EA-91FC-C7463DDC885E"},{"grant_number":"715508","call_identifier":"H2020","_id":"25444568-B435-11E9-9278-68D0E5697425","name":"Probing the Reversibility of Autism Spectrum Disorders by Employing in vivo and in vitro Models"},{"grant_number":"692692","name":"Biophysics and circuit function of a giant cortical glumatergic synapse","_id":"25B7EB9E-B435-11E9-9278-68D0E5697425","call_identifier":"H2020"},{"name":"International IST Doctoral Program","call_identifier":"H2020","_id":"2564DBCA-B435-11E9-9278-68D0E5697425","grant_number":"665385"},{"grant_number":"101026635","call_identifier":"H2020","_id":"fc2be41b-9c52-11eb-aca3-faa90aa144e9","name":"Synaptic computations of the hippocampal CA3 circuitry"}],"contributor":[{"id":"443DB6DE-F248-11E8-B48F-1D18A9856A87","first_name":"Julia M","last_name":"Michalska"},{"last_name":"Lyudchik","first_name":"Julia","id":"46E28B80-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0002-2340-7431","id":"39BDC62C-F248-11E8-B48F-1D18A9856A87","last_name":"Velicky","first_name":"Philipp"},{"first_name":"Hana","last_name":"Stefanickova","id":"ee3cb6ca-ec98-11ea-ae11-ff703e2254ed"},{"first_name":"Jake","last_name":"Watson","orcid":"0000-0002-8698-3823","id":"63836096-4690-11EA-BD4E-32803DDC885E"},{"id":"9ac8f577-2357-11eb-997a-e566c5550886","first_name":"Alban","last_name":"Cenameri"},{"orcid":"0000-0003-1216-9105","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","first_name":"Christoph M","last_name":"Sommer"},{"first_name":"Nicole","last_name":"Amberg","orcid":"0000-0002-3183-8207","id":"4CD6AAC6-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Venturino","first_name":"Alessandro","id":"41CB84B2-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2356-9403"},{"last_name":"Roessler","first_name":"Karl"},{"last_name":"Czech","first_name":"Thomas"},{"first_name":"Romana","last_name":"Höftberger"},{"orcid":"0000-0001-8635-0877","id":"36ACD32E-F248-11E8-B48F-1D18A9856A87","first_name":"Sandra","last_name":"Siegert"},{"first_name":"Gaia","last_name":"Novarino","orcid":"0000-0002-7673-7178","id":"3E57A680-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Peter M","last_name":"Jonas","id":"353C1B58-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5001-4804"}],"doi":"10.15479/AT:ISTA:13126","acknowledgement":"We thank Jakob Vorlaufer, Nathalie Agudelo-Dueñas, Wiebke Jahr, Andreas Wartak for microscope maintenance and troubleshooting, Caroline Kreuzinger, Anna Freeman, and Irene Erber for technical assistance and Matthias Tomschik for support with obtaining human samples. We gratefully acknowledge Eder Miguel for setting up webKnossos and Marek Šuplata for computational support and hardware control. We are grateful to Ryuichi Shigemoto and Bernd Bickel for generous support, and Michael Sixt and Scott Boyd (Stanford University) for discussions and critical reading of the manuscript. PSD95-HaloTag mice were kindly provided by Seth Grant (University of Edinburgh). We acknowledge expert support by IST Austria’s scientific computing, imaging and optics, preclinical, and lab support facilities, and by the Library and Miba machine shop.\r\nWe gratefully acknowledge funding by the following sources: \r\nAustrian Science Fund (FWF) grant I3600-B27 (JGD)\r\nAustrian Science Fund (FWF) grant DK W1232 (JGD, JMM)\r\nAustrian Science Fund (FWF) grant Z 312-B27, Wittgenstein award (PJ)\r\nAustrian Science Funds (FWF) projects I4685-B, I6565-B (SYNABS) and DOC 33-B27 (RH)\r\nGesellschaft für Forschungsförderung NÖ (NFB) grant LSC18-022 (JGD)\r\nEuropean Union’s Horizon 2020 research and innovation programme, European Research Council (ERC) grant 715508 – REVERSEAUTISM (GN)\r\nEuropean Union’s Horizon 2020 research and innovation programme, European Research Council (ERC) grant 692692 – GIANTSYN (PJ)\r\nMarie Skłodowska-Curie Actions Fellowship GA no. 665385 under the EU Horizon 2020 program (JMM, JL)\r\nMarie Skłodowska-Curie Actions Individual Fellowship 101026635 under the EU Horizon 2020 program (JFW)","year":"2023","_id":"13126","month":"08","type":"research_data","oa_version":"Published Version","abstract":[{"text":"Mapping the complex and dense arrangement of cells and their connectivity in brain tissue demands nanoscale spatial resolution imaging. Super-resolution optical microscopy excels at visualizing specific molecules and individual cells but fails to provide tissue context. Here, we developed Comprehensive Analysis of Tissues across Scales (CATS), a technology to densely map brain tissue architecture from millimeter regional to nanometer synaptic scales in diverse chemically fixed brain preparations, including rodent and human. CATS uses fixation-compatible extracellular labeling and optical imaging, including stimulated emission depletion or expansion microscopy, to comprehensively delineate cellular structures. It enables three-dimensional reconstruction of single synapses and mapping of synaptic connectivity by identification and analysis of putative synaptic cleft regions. Applying CATS to the mouse hippocampal mossy fiber circuitry, we reconstructed and quantified the synaptic input and output structure of identified neurons. We furthermore demonstrate applicability to clinically derived human tissue samples, including formalin-fixed paraffin-embedded routine diagnostic specimens, for visualizing the cellular architecture of brain tissue in health and disease.","lang":"eng"}],"date_updated":"2024-02-21T12:18:19Z","date_created":"2023-06-07T07:15:12Z","file_date_updated":"2023-08-04T13:19:47Z","status":"public","related_material":{"link":[{"url":"https://pub.ista.ac.at/group_danzl/data/CATS/","description":"Original data for Fig. 5d, Fig. 5d (N2V) and Fig. 5f-i, provided via an external link due to the large size (>10GB) of the datasets. ","relation":"research_data"}],"record":[{"status":"public","id":"14257","relation":"used_in_publication"}]},"citation":{"apa":"Danzl, J. G. (2023). Research data for the publication “Imaging brain tissue architecture across millimeter to nanometer scales.” Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT:ISTA:13126\">https://doi.org/10.15479/AT:ISTA:13126</a>","ista":"Danzl JG. 2023. Research data for the publication ‘Imaging brain tissue architecture across millimeter to nanometer scales’, Institute of Science and Technology Austria, <a href=\"https://doi.org/10.15479/AT:ISTA:13126\">10.15479/AT:ISTA:13126</a>.","mla":"Danzl, Johann G. <i>Research Data for the Publication “Imaging Brain Tissue Architecture across Millimeter to Nanometer Scales.”</i> Institute of Science and Technology Austria, 2023, doi:<a href=\"https://doi.org/10.15479/AT:ISTA:13126\">10.15479/AT:ISTA:13126</a>.","ama":"Danzl JG. Research data for the publication “Imaging brain tissue architecture across millimeter to nanometer scales.” 2023. doi:<a href=\"https://doi.org/10.15479/AT:ISTA:13126\">10.15479/AT:ISTA:13126</a>","short":"J.G. Danzl, (2023).","chicago":"Danzl, Johann G. “Research Data for the Publication ‘Imaging Brain Tissue Architecture across Millimeter to Nanometer Scales.’” Institute of Science and Technology Austria, 2023. <a href=\"https://doi.org/10.15479/AT:ISTA:13126\">https://doi.org/10.15479/AT:ISTA:13126</a>.","ieee":"J. G. Danzl, “Research data for the publication ‘Imaging brain tissue architecture across millimeter to nanometer scales.’” Institute of Science and Technology Austria, 2023."},"oa":1,"has_accepted_license":"1","ddc":["610"],"date_published":"2023-08-04T00:00:00Z","acknowledged_ssus":[{"_id":"ScienComp"},{"_id":"Bio"},{"_id":"PreCl"},{"_id":"LifeSc"},{"_id":"M-Shop"},{"_id":"E-Lib"}]},{"day":"01","author":[{"first_name":"Philipp","last_name":"Velicky","full_name":"Velicky, Philipp","orcid":"0000-0002-2340-7431","id":"39BDC62C-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Miguel Villalba","first_name":"Eder","full_name":"Miguel Villalba, Eder","id":"3FB91342-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5665-0430"},{"orcid":"0000-0003-3862-1235","id":"443DB6DE-F248-11E8-B48F-1D18A9856A87","full_name":"Michalska, Julia M","first_name":"Julia M","last_name":"Michalska"},{"id":"46E28B80-F248-11E8-B48F-1D18A9856A87","full_name":"Lyudchik, Julia","last_name":"Lyudchik","first_name":"Julia"},{"last_name":"Wei","first_name":"Donglai","full_name":"Wei, Donglai"},{"last_name":"Lin","first_name":"Zudi","full_name":"Lin, Zudi"},{"first_name":"Jake","last_name":"Watson","id":"63836096-4690-11EA-BD4E-32803DDC885E","orcid":"0000-0002-8698-3823","full_name":"Watson, Jake"},{"first_name":"Jakob","last_name":"Troidl","full_name":"Troidl, Jakob"},{"full_name":"Beyer, Johanna","first_name":"Johanna","last_name":"Beyer"},{"id":"43DF3136-F248-11E8-B48F-1D18A9856A87","full_name":"Ben Simon, Yoav","last_name":"Ben Simon","first_name":"Yoav"},{"full_name":"Sommer, Christoph M","orcid":"0000-0003-1216-9105","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","last_name":"Sommer","first_name":"Christoph M"},{"first_name":"Wiebke","last_name":"Jahr","full_name":"Jahr, Wiebke","id":"425C1CE8-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Cenameri, Alban","id":"9ac8f577-2357-11eb-997a-e566c5550886","last_name":"Cenameri","first_name":"Alban"},{"full_name":"Broichhagen, Johannes","last_name":"Broichhagen","first_name":"Johannes"},{"first_name":"Seth G.N.","last_name":"Grant","full_name":"Grant, Seth G.N."},{"first_name":"Peter M","last_name":"Jonas","id":"353C1B58-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5001-4804","full_name":"Jonas, Peter M"},{"orcid":"0000-0002-7673-7178","id":"3E57A680-F248-11E8-B48F-1D18A9856A87","full_name":"Novarino, Gaia","last_name":"Novarino","first_name":"Gaia"},{"full_name":"Pfister, Hanspeter","first_name":"Hanspeter","last_name":"Pfister"},{"orcid":"0000-0001-6511-9385","id":"49876194-F248-11E8-B48F-1D18A9856A87","full_name":"Bickel, Bernd","last_name":"Bickel","first_name":"Bernd"},{"last_name":"Danzl","first_name":"Johann G","orcid":"0000-0001-8559-3973","id":"42EFD3B6-F248-11E8-B48F-1D18A9856A87","full_name":"Danzl, Johann G"}],"title":"Dense 4D nanoscale reconstruction of living brain tissue","department":[{"_id":"PeJo"},{"_id":"GaNo"},{"_id":"BeBi"},{"_id":"JoDa"},{"_id":"Bio"}],"pmid":1,"publisher":"Springer Nature","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","article_type":"original","scopus_import":"1","article_processing_charge":"Yes","ec_funded":1,"publication":"Nature Methods","publication_identifier":{"issn":["1548-7091"],"eissn":["1548-7105"]},"quality_controlled":"1","doi":"10.1038/s41592-023-01936-6","project":[{"_id":"265CB4D0-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","name":"Optical control of synaptic function via adhesion molecules","grant_number":"I03600"},{"grant_number":"W1232-B24","name":"Molecular Drug Targets","_id":"2548AE96-B435-11E9-9278-68D0E5697425","call_identifier":"FWF"},{"grant_number":"Z00312","_id":"25C5A090-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","name":"The Wittgenstein Prize"},{"name":"High content imaging to decode human immune cell interactions in health and allergic disease","_id":"23889792-32DE-11EA-91FC-C7463DDC885E"},{"grant_number":"665385","name":"International IST Doctoral Program","call_identifier":"H2020","_id":"2564DBCA-B435-11E9-9278-68D0E5697425"},{"grant_number":"715767","_id":"24F9549A-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"MATERIALIZABLE: Intelligent fabrication-oriented Computational Design and Modeling"},{"call_identifier":"H2020","_id":"25444568-B435-11E9-9278-68D0E5697425","name":"Probing the Reversibility of Autism Spectrum Disorders by Employing in vivo and in vitro Models","grant_number":"715508"},{"grant_number":"692692","_id":"25B7EB9E-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"Biophysics and circuit function of a giant cortical glumatergic synapse"},{"name":"Synaptic computations of the hippocampal CA3 circuitry","_id":"fc2be41b-9c52-11eb-aca3-faa90aa144e9","call_identifier":"H2020","grant_number":"101026635"},{"grant_number":"LT00057","_id":"2668BFA0-B435-11E9-9278-68D0E5697425","name":"High-speed 3D-nanoscopy to study the role of adhesion during 3D cell migration"}],"language":[{"iso":"eng"}],"isi":1,"month":"08","type":"journal_article","oa_version":"Published Version","abstract":[{"text":"Three-dimensional (3D) reconstruction of living brain tissue down to an individual synapse level would create opportunities for decoding the dynamics and structure–function relationships of the brain’s complex and dense information processing network; however, this has been hindered by insufficient 3D resolution, inadequate signal-to-noise ratio and prohibitive light burden in optical imaging, whereas electron microscopy is inherently static. Here we solved these challenges by developing an integrated optical/machine-learning technology, LIONESS (live information-optimized nanoscopy enabling saturated segmentation). This leverages optical modifications to stimulated emission depletion microscopy in comprehensively, extracellularly labeled tissue and previous information on sample structure via machine learning to simultaneously achieve isotropic super-resolution, high signal-to-noise ratio and compatibility with living tissue. This allows dense deep-learning-based instance segmentation and 3D reconstruction at a synapse level, incorporating molecular, activity and morphodynamic information. LIONESS opens up avenues for studying the dynamic functional (nano-)architecture of living brain tissue.","lang":"eng"}],"date_updated":"2024-01-10T08:37:48Z","page":"1256-1265","date_created":"2023-07-23T22:01:13Z","volume":20,"year":"2023","acknowledgement":"We thank J. Vorlaufer, N. Agudelo and A. Wartak for microscope maintenance and troubleshooting, C. Kreuzinger and A. Freeman for technical assistance, M. Šuplata for hardware control support and M. Cunha dos Santos for initial exploration of software. We\r\nthank P. Henderson for advice on deep-learning training and M. Sixt, S. Boyd and T. Weiss for discussions and critical reading of the manuscript. L. Lavis (Janelia Research Campus) generously provided the JF585-HaloTag ligand. We acknowledge expert support by IST\r\nAustria’s scientific computing, imaging and optics, preclinical, library and laboratory support facilities and by the Miba machine shop. We gratefully acknowledge funding by the following sources: Austrian Science Fund (F.W.F.) grant no. I3600-B27 (J.G.D.), grant no. DK W1232\r\n(J.G.D. and J.M.M.) and grant no. Z 312-B27, Wittgenstein award (P.J.); the Gesellschaft für Forschungsförderung NÖ grant no. LSC18-022 (J.G.D.); an ISTA Interdisciplinary project grant (J.G.D. and B.B.); the European Union’s Horizon 2020 research and innovation programme,\r\nMarie-Skłodowska Curie grant 665385 (J.M.M. and J.L.); the European Union’s Horizon 2020 research and innovation programme, European Research Council grant no. 715767, MATERIALIZABLE (B.B.); grant no. 715508, REVERSEAUTISM (G.N.); grant no. 695568, SYNNOVATE (S.G.N.G.); and grant no. 692692, GIANTSYN (P.J.); the Simons\r\nFoundation Autism Research Initiative grant no. 529085 (S.G.N.G.); the Wellcome Trust Technology Development grant no. 202932 (S.G.N.G.); the Marie Skłodowska-Curie Actions Individual Fellowship no. 101026635 under the EU Horizon 2020 program (J.F.W.);\r\nthe Human Frontier Science Program postdoctoral fellowship LT000557/2018 (W.J.); and the National Science Foundation grant no. IIS-1835231 (H.P.) and NCS-FO-2124179 (H.P.).","_id":"13267","publication_status":"published","oa":1,"acknowledged_ssus":[{"_id":"ScienComp"},{"_id":"Bio"},{"_id":"PreCl"},{"_id":"E-Lib"},{"_id":"LifeSc"},{"_id":"M-Shop"}],"main_file_link":[{"url":"https://doi.org/10.1038/s41592-023-01936-6","open_access":"1"}],"date_published":"2023-08-01T00:00:00Z","external_id":{"pmid":["37429995"],"isi":["001025621500001"]},"status":"public","citation":{"short":"P. Velicky, E. Miguel Villalba, J.M. Michalska, J. Lyudchik, D. Wei, Z. Lin, J. Watson, J. Troidl, J. Beyer, Y. Ben Simon, C.M. Sommer, W. Jahr, A. Cenameri, J. Broichhagen, S.G.N. Grant, P.M. Jonas, G. Novarino, H. Pfister, B. Bickel, J.G. Danzl, Nature Methods 20 (2023) 1256–1265.","chicago":"Velicky, Philipp, Eder Miguel Villalba, Julia M Michalska, Julia Lyudchik, Donglai Wei, Zudi Lin, Jake Watson, et al. “Dense 4D Nanoscale Reconstruction of Living Brain Tissue.” <i>Nature Methods</i>. Springer Nature, 2023. <a href=\"https://doi.org/10.1038/s41592-023-01936-6\">https://doi.org/10.1038/s41592-023-01936-6</a>.","ieee":"P. Velicky <i>et al.</i>, “Dense 4D nanoscale reconstruction of living brain tissue,” <i>Nature Methods</i>, vol. 20. Springer Nature, pp. 1256–1265, 2023.","apa":"Velicky, P., Miguel Villalba, E., Michalska, J. M., Lyudchik, J., Wei, D., Lin, Z., … Danzl, J. G. (2023). Dense 4D nanoscale reconstruction of living brain tissue. <i>Nature Methods</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41592-023-01936-6\">https://doi.org/10.1038/s41592-023-01936-6</a>","ista":"Velicky P, Miguel Villalba E, Michalska JM, Lyudchik J, Wei D, Lin Z, Watson J, Troidl J, Beyer J, Ben Simon Y, Sommer CM, Jahr W, Cenameri A, Broichhagen J, Grant SGN, Jonas PM, Novarino G, Pfister H, Bickel B, Danzl JG. 2023. Dense 4D nanoscale reconstruction of living brain tissue. Nature Methods. 20, 1256–1265.","mla":"Velicky, Philipp, et al. “Dense 4D Nanoscale Reconstruction of Living Brain Tissue.” <i>Nature Methods</i>, vol. 20, Springer Nature, 2023, pp. 1256–65, doi:<a href=\"https://doi.org/10.1038/s41592-023-01936-6\">10.1038/s41592-023-01936-6</a>.","ama":"Velicky P, Miguel Villalba E, Michalska JM, et al. Dense 4D nanoscale reconstruction of living brain tissue. <i>Nature Methods</i>. 2023;20:1256-1265. doi:<a href=\"https://doi.org/10.1038/s41592-023-01936-6\">10.1038/s41592-023-01936-6</a>"},"related_material":{"link":[{"relation":"software","url":"https://github.com/danzllab/LIONESS"}],"record":[{"relation":"research_data","id":"12817","status":"public"},{"relation":"shorter_version","status":"public","id":"14770"}]},"intvolume":"        20"},{"project":[{"call_identifier":"FWF","_id":"265CB4D0-B435-11E9-9278-68D0E5697425","name":"Optical control of synaptic function via adhesion molecules","grant_number":"I03600"},{"grant_number":"W1232-B24","_id":"2548AE96-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","name":"Molecular Drug Targets"},{"_id":"25C5A090-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","name":"The Wittgenstein Prize","grant_number":"Z00312"},{"name":"High content imaging to decode human immune cell interactions in health and allergic disease","_id":"23889792-32DE-11EA-91FC-C7463DDC885E"},{"grant_number":"715508","call_identifier":"H2020","_id":"25444568-B435-11E9-9278-68D0E5697425","name":"Probing the Reversibility of Autism Spectrum Disorders by Employing in vivo and in vitro Models"},{"grant_number":"692692","_id":"25B7EB9E-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"Biophysics and circuit function of a giant cortical glumatergic synapse"},{"grant_number":"665385","name":"International IST Doctoral Program","call_identifier":"H2020","_id":"2564DBCA-B435-11E9-9278-68D0E5697425"},{"_id":"fc2be41b-9c52-11eb-aca3-faa90aa144e9","call_identifier":"H2020","name":"Synaptic computations of the hippocampal CA3 circuitry","grant_number":"101026635"}],"language":[{"iso":"eng"}],"isi":1,"publication_identifier":{"issn":["1087-0156"],"eissn":["1546-1696"]},"quality_controlled":"1","doi":"10.1038/s41587-023-01911-8","department":[{"_id":"SaSi"},{"_id":"GaNo"},{"_id":"PeJo"},{"_id":"JoDa"},{"_id":"Bio"},{"_id":"RySh"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publisher":"Springer Nature","article_type":"original","article_processing_charge":"Yes (in subscription journal)","ec_funded":1,"scopus_import":"1","publication":"Nature Biotechnology","day":"31","author":[{"last_name":"Michalska","first_name":"Julia M","id":"443DB6DE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-3862-1235","full_name":"Michalska, Julia M"},{"id":"46E28B80-F248-11E8-B48F-1D18A9856A87","full_name":"Lyudchik, Julia","last_name":"Lyudchik","first_name":"Julia"},{"first_name":"Philipp","last_name":"Velicky","full_name":"Velicky, Philipp","orcid":"0000-0002-2340-7431","id":"39BDC62C-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Korinkova","first_name":"Hana","id":"ee3cb6ca-ec98-11ea-ae11-ff703e2254ed","full_name":"Korinkova, Hana"},{"last_name":"Watson","first_name":"Jake","orcid":"0000-0002-8698-3823","id":"63836096-4690-11EA-BD4E-32803DDC885E","full_name":"Watson, Jake"},{"last_name":"Cenameri","first_name":"Alban","id":"9ac8f577-2357-11eb-997a-e566c5550886","full_name":"Cenameri, Alban"},{"first_name":"Christoph M","last_name":"Sommer","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-1216-9105","full_name":"Sommer, Christoph M"},{"first_name":"Nicole","last_name":"Amberg","full_name":"Amberg, Nicole","id":"4CD6AAC6-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-3183-8207"},{"full_name":"Venturino, Alessandro","id":"41CB84B2-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2356-9403","last_name":"Venturino","first_name":"Alessandro"},{"first_name":"Karl","last_name":"Roessler","full_name":"Roessler, Karl"},{"last_name":"Czech","first_name":"Thomas","full_name":"Czech, Thomas"},{"first_name":"Romana","last_name":"Höftberger","full_name":"Höftberger, Romana"},{"last_name":"Siegert","first_name":"Sandra","full_name":"Siegert, Sandra","id":"36ACD32E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8635-0877"},{"last_name":"Novarino","first_name":"Gaia","full_name":"Novarino, Gaia","id":"3E57A680-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-7673-7178"},{"id":"353C1B58-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5001-4804","full_name":"Jonas, Peter M","first_name":"Peter M","last_name":"Jonas"},{"last_name":"Danzl","first_name":"Johann G","full_name":"Danzl, Johann G","orcid":"0000-0001-8559-3973","id":"42EFD3B6-F248-11E8-B48F-1D18A9856A87"}],"title":"Imaging brain tissue architecture across millimeter to nanometer scales","status":"public","external_id":{"isi":["001065254200001"]},"citation":{"ama":"Michalska JM, Lyudchik J, Velicky P, et al. Imaging brain tissue architecture across millimeter to nanometer scales. <i>Nature Biotechnology</i>. 2023. doi:<a href=\"https://doi.org/10.1038/s41587-023-01911-8\">10.1038/s41587-023-01911-8</a>","apa":"Michalska, J. M., Lyudchik, J., Velicky, P., Korinkova, H., Watson, J., Cenameri, A., … Danzl, J. G. (2023). Imaging brain tissue architecture across millimeter to nanometer scales. <i>Nature Biotechnology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41587-023-01911-8\">https://doi.org/10.1038/s41587-023-01911-8</a>","ista":"Michalska JM, Lyudchik J, Velicky P, Korinkova H, Watson J, Cenameri A, Sommer CM, Amberg N, Venturino A, Roessler K, Czech T, Höftberger R, Siegert S, Novarino G, Jonas PM, Danzl JG. 2023. Imaging brain tissue architecture across millimeter to nanometer scales. Nature Biotechnology.","mla":"Michalska, Julia M., et al. “Imaging Brain Tissue Architecture across Millimeter to Nanometer Scales.” <i>Nature Biotechnology</i>, Springer Nature, 2023, doi:<a href=\"https://doi.org/10.1038/s41587-023-01911-8\">10.1038/s41587-023-01911-8</a>.","chicago":"Michalska, Julia M, Julia Lyudchik, Philipp Velicky, Hana Korinkova, Jake Watson, Alban Cenameri, Christoph M Sommer, et al. “Imaging Brain Tissue Architecture across Millimeter to Nanometer Scales.” <i>Nature Biotechnology</i>. Springer Nature, 2023. <a href=\"https://doi.org/10.1038/s41587-023-01911-8\">https://doi.org/10.1038/s41587-023-01911-8</a>.","ieee":"J. M. Michalska <i>et al.</i>, “Imaging brain tissue architecture across millimeter to nanometer scales,” <i>Nature Biotechnology</i>. Springer Nature, 2023.","short":"J.M. Michalska, J. Lyudchik, P. Velicky, H. Korinkova, J. Watson, A. Cenameri, C.M. Sommer, N. Amberg, A. Venturino, K. Roessler, T. Czech, R. Höftberger, S. Siegert, G. Novarino, P.M. Jonas, J.G. Danzl, Nature Biotechnology (2023)."},"related_material":{"link":[{"relation":"software","url":"https://github.com/danzllab/CATS"}],"record":[{"relation":"research_data","status":"public","id":"13126"}]},"publication_status":"epub_ahead","oa":1,"acknowledged_ssus":[{"_id":"ScienComp"},{"_id":"Bio"},{"_id":"PreCl"},{"_id":"LifeSc"},{"_id":"M-Shop"},{"_id":"E-Lib"}],"main_file_link":[{"url":"https://doi.org/10.1038/s41587-023-01911-8","open_access":"1"}],"date_published":"2023-08-31T00:00:00Z","year":"2023","acknowledgement":"We thank J. Vorlaufer, N. Agudelo-Dueñas, W. Jahr and A. Wartak for microscope maintenance and troubleshooting; C. Kreuzinger, A. Freeman and I. Erber for technical assistance; and M. Tomschik for support with obtaining human samples. We gratefully acknowledge E. Miguel for setting up webKnossos and M. Šuplata for computational support and hardware control. We are grateful to R. Shigemoto and B. Bickel for generous support and M. Sixt and S. Boyd (Stanford University) for discussions and critical reading of the paper. PSD95-HaloTag mice were kindly provided by S. Grant (University of Edinburgh). We acknowledge expert support by Institute of Science and Technology Austria’s scientific computing, imaging and optics, preclinical and lab support facilities and by the Miba machine shop and library. We gratefully acknowledge funding by the following sources: Austrian Science Fund (FWF) grant I3600-B27 (J.G.D.); Austrian Science Fund (FWF) grant DK W1232 (J.G.D. and J.M.M.); Austrian Science Fund (FWF) grant Z 312-B27, Wittgenstein award (P.J.); Austrian Science Fund (FWF) projects I4685-B, I6565-B (SYNABS) and DOC 33-B27 (R.H.); Gesellschaft für Forschungsförderung NÖ (NFB) grant LSC18-022 (J.G.D.); European Union’s Horizon 2020 research and innovation programme, European Research Council (ERC) grant 715508 – REVERSEAUTISM (G.N.); European Union’s Horizon 2020 research and innovation programme, European Research Council (ERC) grant 692692 – GIANTSYN (P.J.); Marie Skłodowska-Curie Actions Fellowship GA no. 665385 under the EU Horizon 2020 program (J.M.M. and J.L.); and Marie Skłodowska-Curie Actions Individual Fellowship no. 101026635 under the EU Horizon 2020 program (J.F.W.).","_id":"14257","oa_version":"Published Version","type":"journal_article","month":"08","abstract":[{"lang":"eng","text":"Mapping the complex and dense arrangement of cells and their connectivity in brain tissue demands nanoscale spatial resolution imaging. Super-resolution optical microscopy excels at visualizing specific molecules and individual cells but fails to provide tissue context. Here we developed Comprehensive Analysis of Tissues across Scales (CATS), a technology to densely map brain tissue architecture from millimeter regional to nanometer synaptic scales in diverse chemically fixed brain preparations, including rodent and human. CATS uses fixation-compatible extracellular labeling and optical imaging, including stimulated emission depletion or expansion microscopy, to comprehensively delineate cellular structures. It enables three-dimensional reconstruction of single synapses and mapping of synaptic connectivity by identification and analysis of putative synaptic cleft regions. Applying CATS to the mouse hippocampal mossy fiber circuitry, we reconstructed and quantified the synaptic input and output structure of identified neurons. We furthermore demonstrate applicability to clinically derived human tissue samples, including formalin-fixed paraffin-embedded routine diagnostic specimens, for visualizing the cellular architecture of brain tissue in health and disease."}],"date_updated":"2024-02-21T12:18:18Z","date_created":"2023-09-03T22:01:15Z"},{"isi":1,"keyword":["General Biochemistry","Genetics and Molecular Biology"],"language":[{"iso":"eng"}],"issue":"9","project":[{"grant_number":"W1232-B24","name":"Molecular Drug Targets","_id":"2548AE96-B435-11E9-9278-68D0E5697425","call_identifier":"FWF"},{"grant_number":"725780","name":"Principles of Neural Stem Cell Lineage Progression in Cerebral Cortex Development","_id":"260018B0-B435-11E9-9278-68D0E5697425","call_identifier":"H2020"},{"grant_number":"715508","call_identifier":"H2020","_id":"25444568-B435-11E9-9278-68D0E5697425","name":"Probing the Reversibility of Autism Spectrum Disorders by Employing in vivo and in vitro Models"}],"doi":"10.1016/j.cell.2023.02.037","quality_controlled":"1","publication_identifier":{"issn":["0092-8674"]},"publication":"Cell","ec_funded":1,"scopus_import":"1","article_processing_charge":"Yes (via OA deal)","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"article_type":"original","publisher":"Elsevier","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","department":[{"_id":"SiHi"},{"_id":"GaNo"}],"title":"Large neutral amino acid levels tune perinatal neuronal excitability and survival","author":[{"full_name":"Knaus, Lisa","id":"3B2ABCF4-F248-11E8-B48F-1D18A9856A87","first_name":"Lisa","last_name":"Knaus"},{"full_name":"Basilico, Bernadette","id":"36035796-5ACA-11E9-A75E-7AF2E5697425","orcid":"0000-0003-1843-3173","last_name":"Basilico","first_name":"Bernadette"},{"first_name":"Daniel","last_name":"Malzl","full_name":"Malzl, Daniel"},{"last_name":"Gerykova Bujalkova","first_name":"Maria","full_name":"Gerykova Bujalkova, Maria"},{"full_name":"Smogavec, Mateja","last_name":"Smogavec","first_name":"Mateja"},{"full_name":"Schwarz, Lena A.","first_name":"Lena A.","last_name":"Schwarz"},{"id":"f141a35d-15a9-11ec-9fb2-fef6becc7b6f","full_name":"Gorkiewicz, Sarah","last_name":"Gorkiewicz","first_name":"Sarah"},{"first_name":"Nicole","last_name":"Amberg","id":"4CD6AAC6-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-3183-8207","full_name":"Amberg, Nicole"},{"full_name":"Pauler, Florian","orcid":"0000-0002-7462-0048","id":"48EA0138-F248-11E8-B48F-1D18A9856A87","first_name":"Florian","last_name":"Pauler"},{"full_name":"Knittl-Frank, Christian","first_name":"Christian","last_name":"Knittl-Frank"},{"last_name":"Tassinari","first_name":"Marianna","id":"7af593f1-d44a-11ed-bf94-a3646a6bb35e","full_name":"Tassinari, Marianna"},{"full_name":"Maulide, Nuno","last_name":"Maulide","first_name":"Nuno"},{"first_name":"Thomas","last_name":"Rülicke","full_name":"Rülicke, Thomas"},{"last_name":"Menche","first_name":"Jörg","full_name":"Menche, Jörg"},{"first_name":"Simon","last_name":"Hippenmeyer","full_name":"Hippenmeyer, Simon","orcid":"0000-0003-2279-1061","id":"37B36620-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0002-7673-7178","id":"3E57A680-F248-11E8-B48F-1D18A9856A87","full_name":"Novarino, Gaia","last_name":"Novarino","first_name":"Gaia"}],"day":"27","file":[{"content_type":"application/pdf","relation":"main_file","file_size":15712841,"creator":"dernst","success":1,"file_name":"2023_Cell_Knaus.pdf","date_created":"2023-05-02T09:26:21Z","access_level":"open_access","date_updated":"2023-05-02T09:26:21Z","file_id":"12889","checksum":"47e94fbe19e86505b429cb7a5b503ce6"}],"intvolume":"       186","related_material":{"link":[{"relation":"press_release","url":"https://ista.ac.at/en/news/feed-them-or-lose-them/","description":"News on ISTA Website"}],"record":[{"id":"13107","status":"public","relation":"dissertation_contains"}]},"citation":{"ama":"Knaus L, Basilico B, Malzl D, et al. Large neutral amino acid levels tune perinatal neuronal excitability and survival. <i>Cell</i>. 2023;186(9):1950-1967.e25. doi:<a href=\"https://doi.org/10.1016/j.cell.2023.02.037\">10.1016/j.cell.2023.02.037</a>","mla":"Knaus, Lisa, et al. “Large Neutral Amino Acid Levels Tune Perinatal Neuronal Excitability and Survival.” <i>Cell</i>, vol. 186, no. 9, Elsevier, 2023, p. 1950–1967.e25, doi:<a href=\"https://doi.org/10.1016/j.cell.2023.02.037\">10.1016/j.cell.2023.02.037</a>.","ista":"Knaus L, Basilico B, Malzl D, Gerykova Bujalkova M, Smogavec M, Schwarz LA, Gorkiewicz S, Amberg N, Pauler F, Knittl-Frank C, Tassinari M, Maulide N, Rülicke T, Menche J, Hippenmeyer S, Novarino G. 2023. Large neutral amino acid levels tune perinatal neuronal excitability and survival. Cell. 186(9), 1950–1967.e25.","apa":"Knaus, L., Basilico, B., Malzl, D., Gerykova Bujalkova, M., Smogavec, M., Schwarz, L. A., … Novarino, G. (2023). Large neutral amino acid levels tune perinatal neuronal excitability and survival. <i>Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cell.2023.02.037\">https://doi.org/10.1016/j.cell.2023.02.037</a>","ieee":"L. Knaus <i>et al.</i>, “Large neutral amino acid levels tune perinatal neuronal excitability and survival,” <i>Cell</i>, vol. 186, no. 9. Elsevier, p. 1950–1967.e25, 2023.","chicago":"Knaus, Lisa, Bernadette Basilico, Daniel Malzl, Maria Gerykova Bujalkova, Mateja Smogavec, Lena A. Schwarz, Sarah Gorkiewicz, et al. “Large Neutral Amino Acid Levels Tune Perinatal Neuronal Excitability and Survival.” <i>Cell</i>. Elsevier, 2023. <a href=\"https://doi.org/10.1016/j.cell.2023.02.037\">https://doi.org/10.1016/j.cell.2023.02.037</a>.","short":"L. Knaus, B. Basilico, D. Malzl, M. Gerykova Bujalkova, M. Smogavec, L.A. Schwarz, S. Gorkiewicz, N. Amberg, F. Pauler, C. Knittl-Frank, M. Tassinari, N. Maulide, T. Rülicke, J. Menche, S. Hippenmeyer, G. Novarino, Cell 186 (2023) 1950–1967.e25."},"status":"public","external_id":{"isi":["000991468700001"]},"date_published":"2023-04-27T00:00:00Z","ddc":["570"],"acknowledged_ssus":[{"_id":"PreCl"},{"_id":"EM-Fac"},{"_id":"Bio"},{"_id":"LifeSc"}],"oa":1,"publication_status":"published","has_accepted_license":"1","_id":"12802","acknowledgement":"We thank A. Freeman and V. Voronin for technical assistance, S. Deixler, A. Stichelberger, M. Schunn, and the Preclinical Facility for managing our animal colony. We thank L. Andersen and J. Sonntag, who were involved in generating the MADM lines. We thank the ISTA LSF Mass Spectrometry Core Facility for assistance with the proteomic analysis, as well as the ISTA electron microscopy and Imaging and Optics facility for technical support. Metabolomics LC-MS/MS analysis was performed by the Metabolomics Facility at Vienna BioCenter Core Facilities (VBCF). We acknowledge the support of the EMBL Metabolomics Core Facility (MCF) for lipidomics and intracellular metabolomics mass spectrometry data acquisition and analysis. RNA sequencing was performed by the Next Generation Sequencing Facility at VBCF. Schematics were generated using Biorender.com. This work was supported by the Austrian Science Fund (FWF, DK W1232-B24) and by the European Union’s Horizon 2020 research and innovation program (ERC) grant 725780 (LinPro) to S.H. and 715508 (REVERSEAUTISM) to G.N.","year":"2023","volume":186,"file_date_updated":"2023-05-02T09:26:21Z","date_created":"2023-04-05T08:15:40Z","page":"1950-1967.e25","date_updated":"2024-02-07T08:03:32Z","abstract":[{"text":"Little is known about the critical metabolic changes that neural cells have to undergo during development and how temporary shifts in this program can influence brain circuitries and behavior. Inspired by the discovery that mutations in SLC7A5, a transporter of metabolically essential large neutral amino acids (LNAAs), lead to autism, we employed metabolomic profiling to study the metabolic states of the cerebral cortex across different developmental stages. We found that the forebrain undergoes significant metabolic remodeling throughout development, with certain groups of metabolites showing stage-specific changes, but what are the consequences of perturbing this metabolic program? By manipulating Slc7a5 expression in neural cells, we found that the metabolism of LNAAs and lipids are interconnected in the cortex. Deletion of Slc7a5 in neurons affects the postnatal metabolic state, leading to a shift in lipid metabolism. Additionally, it causes stage- and cell-type-specific alterations in neuronal activity patterns, resulting in a long-term circuit dysfunction.","lang":"eng"}],"oa_version":"Published Version","type":"journal_article","month":"04"},{"title":"CHD8 haploinsufficiency links autism to transient alterations in excitatory and inhibitory trajectories","article_number":"110615","file":[{"access_level":"open_access","date_created":"2022-04-15T09:06:25Z","checksum":"b4e8d68f0268dec499af333e6fd5d8e1","date_updated":"2022-04-15T09:06:25Z","file_id":"11164","creator":"dernst","relation":"main_file","content_type":"application/pdf","file_size":"7808644","success":1,"file_name":"2022_CellReports_Villa.pdf"}],"day":"05","author":[{"last_name":"Villa","first_name":"Carlo Emanuele","full_name":"Villa, Carlo Emanuele"},{"last_name":"Cheroni","first_name":"Cristina","full_name":"Cheroni, Cristina"},{"full_name":"Dotter, Christoph","id":"4C66542E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-9033-9096","first_name":"Christoph","last_name":"Dotter"},{"full_name":"López-Tóbon, Alejandro","last_name":"López-Tóbon","first_name":"Alejandro"},{"last_name":"Oliveira","first_name":"Bárbara","id":"3B03AA1A-F248-11E8-B48F-1D18A9856A87","full_name":"Oliveira, Bárbara"},{"full_name":"Sacco, Roberto","id":"42C9F57E-F248-11E8-B48F-1D18A9856A87","last_name":"Sacco","first_name":"Roberto"},{"first_name":"Aysan Çerağ","last_name":"Yahya","id":"365A65F8-F248-11E8-B48F-1D18A9856A87","full_name":"Yahya, Aysan Çerağ"},{"full_name":"Morandell, Jasmin","id":"4739D480-F248-11E8-B48F-1D18A9856A87","last_name":"Morandell","first_name":"Jasmin"},{"full_name":"Gabriele, Michele","last_name":"Gabriele","first_name":"Michele"},{"id":"3A0A06F4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-7667-6854","full_name":"Tavakoli, Mojtaba","first_name":"Mojtaba","last_name":"Tavakoli"},{"id":"46E28B80-F248-11E8-B48F-1D18A9856A87","full_name":"Lyudchik, Julia","first_name":"Julia","last_name":"Lyudchik"},{"first_name":"Christoph M","last_name":"Sommer","orcid":"0000-0003-1216-9105","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","full_name":"Sommer, Christoph M"},{"full_name":"Gabitto, Mariano","first_name":"Mariano","last_name":"Gabitto"},{"full_name":"Danzl, Johann G","orcid":"0000-0001-8559-3973","id":"42EFD3B6-F248-11E8-B48F-1D18A9856A87","last_name":"Danzl","first_name":"Johann G"},{"full_name":"Testa, Giuseppe","first_name":"Giuseppe","last_name":"Testa"},{"id":"3E57A680-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-7673-7178","full_name":"Novarino, Gaia","last_name":"Novarino","first_name":"Gaia"}],"ec_funded":1,"article_processing_charge":"Yes","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"article_type":"original","publication":"Cell Reports","pmid":1,"department":[{"_id":"JoDa"},{"_id":"GaNo"}],"user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","publisher":"Elsevier","quality_controlled":"1","doi":"10.1016/j.celrep.2022.110615","publication_identifier":{"issn":["2211-1247"]},"language":[{"iso":"eng"}],"issue":"1","isi":1,"keyword":["General Biochemistry","Genetics and Molecular Biology"],"project":[{"grant_number":"715508","_id":"25444568-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"Probing the Reversibility of Autism Spectrum Disorders by Employing in vivo and in vitro Models"},{"name":"Identification of converging Molecular Pathways Across Chromatinopathies as Targets for Therapy","_id":"2690FEAC-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","grant_number":"I04205"}],"date_created":"2022-04-15T09:03:10Z","file_date_updated":"2022-04-15T09:06:25Z","volume":39,"date_updated":"2024-03-25T23:30:25Z","abstract":[{"lang":"eng","text":"Mutations in the chromodomain helicase DNA-binding 8 (CHD8) gene are a frequent cause of autism spectrum disorder (ASD). While its phenotypic spectrum often encompasses macrocephaly, implicating cortical abnormalities, how CHD8 haploinsufficiency affects neurodevelopmental is unclear. Here, employing human cerebral organoids, we find that CHD8 haploinsufficiency disrupted neurodevelopmental trajectories with an accelerated and delayed generation of, respectively, inhibitory and excitatory neurons that yields, at days 60 and 120, symmetrically opposite expansions in their proportions. This imbalance is consistent with an enlargement of cerebral organoids as an in vitro correlate of patients’ macrocephaly. Through an isogenic design of patient-specific mutations and mosaic organoids, we define genotype-phenotype relationships and uncover their cell-autonomous nature. Our results define cell-type-specific CHD8-dependent molecular defects related to an abnormal program of proliferation and alternative splicing. By identifying cell-type-specific effects of CHD8 mutations, our study uncovers reproducible developmental alterations that may be employed for neurodevelopmental disease modeling."}],"oa_version":"Published Version","type":"journal_article","month":"04","_id":"11160","year":"2022","acknowledgement":"We thank Farnaz Freeman for technical assistance. This research was supported by the Scientific Service Units (SSU) of IST Austria through resources provided by the Bioimaging Facility (BIF) and the Life Science Facility (LSF). This work supported by the European Union’s Horizon 2020 research and innovation program (ERC) grant 715508 to G.N. (REVERSEAUTISM) and grant 825759 to G.T. (ENDpoiNTs); the Fondazione Cariplo 2017-0886 to A.L.T.; E-Rare-3 JTC 2018 IMPACT to M. Gabriele; and the Austrian Science Fund FWF I 4205-B to G.N. Graphical abstract and figures were created using BioRender.com.","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"ddc":["570"],"date_published":"2022-04-05T00:00:00Z","has_accepted_license":"1","oa":1,"publication_status":"published","citation":{"short":"C.E. Villa, C. Cheroni, C. Dotter, A. López-Tóbon, B. Oliveira, R. Sacco, A.Ç. Yahya, J. Morandell, M. Gabriele, M. Tavakoli, J. Lyudchik, C.M. Sommer, M. Gabitto, J.G. Danzl, G. Testa, G. Novarino, Cell Reports 39 (2022).","chicago":"Villa, Carlo Emanuele, Cristina Cheroni, Christoph Dotter, Alejandro López-Tóbon, Bárbara Oliveira, Roberto Sacco, Aysan Çerağ Yahya, et al. “CHD8 Haploinsufficiency Links Autism to Transient Alterations in Excitatory and Inhibitory Trajectories.” <i>Cell Reports</i>. Elsevier, 2022. <a href=\"https://doi.org/10.1016/j.celrep.2022.110615\">https://doi.org/10.1016/j.celrep.2022.110615</a>.","ieee":"C. E. Villa <i>et al.</i>, “CHD8 haploinsufficiency links autism to transient alterations in excitatory and inhibitory trajectories,” <i>Cell Reports</i>, vol. 39, no. 1. Elsevier, 2022.","apa":"Villa, C. E., Cheroni, C., Dotter, C., López-Tóbon, A., Oliveira, B., Sacco, R., … Novarino, G. (2022). CHD8 haploinsufficiency links autism to transient alterations in excitatory and inhibitory trajectories. <i>Cell Reports</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.celrep.2022.110615\">https://doi.org/10.1016/j.celrep.2022.110615</a>","ista":"Villa CE, Cheroni C, Dotter C, López-Tóbon A, Oliveira B, Sacco R, Yahya AÇ, Morandell J, Gabriele M, Tavakoli M, Lyudchik J, Sommer CM, Gabitto M, Danzl JG, Testa G, Novarino G. 2022. CHD8 haploinsufficiency links autism to transient alterations in excitatory and inhibitory trajectories. Cell Reports. 39(1), 110615.","mla":"Villa, Carlo Emanuele, et al. “CHD8 Haploinsufficiency Links Autism to Transient Alterations in Excitatory and Inhibitory Trajectories.” <i>Cell Reports</i>, vol. 39, no. 1, 110615, Elsevier, 2022, doi:<a href=\"https://doi.org/10.1016/j.celrep.2022.110615\">10.1016/j.celrep.2022.110615</a>.","ama":"Villa CE, Cheroni C, Dotter C, et al. CHD8 haploinsufficiency links autism to transient alterations in excitatory and inhibitory trajectories. <i>Cell Reports</i>. 2022;39(1). doi:<a href=\"https://doi.org/10.1016/j.celrep.2022.110615\">10.1016/j.celrep.2022.110615</a>"},"related_material":{"record":[{"id":"12364","status":"public","relation":"dissertation_contains"}]},"intvolume":"        39","status":"public","external_id":{"isi":["000785983900003"],"pmid":["35385734"]}},{"language":[{"iso":"eng"}],"project":[{"_id":"254BA948-B435-11E9-9278-68D0E5697425","name":"Probing development and reversibility of autism spectrum disorders","grant_number":"401299"},{"grant_number":"707964","_id":"9B91375C-BA93-11EA-9121-9846C619BF3A","name":"Critical windows and reversibility of ASD associated with mutations in chromatin remodelers"},{"grant_number":"715508","call_identifier":"H2020","_id":"25444568-B435-11E9-9278-68D0E5697425","name":"Probing the Reversibility of Autism Spectrum Disorders by Employing in vivo and in vitro Models"},{"name":"Identification of converging Molecular Pathways Across Chromatinopathies as Targets for Therapy","_id":"2690FEAC-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","grant_number":"I04205"}],"doi":"10.15479/at:ista:12094","publication_identifier":{"issn":["2663-337X"]},"article_processing_charge":"No","ec_funded":1,"user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","publisher":"Institute of Science and Technology Austria","department":[{"_id":"GradSch"},{"_id":"GaNo"}],"title":"Transcriptional consequences of mutations in genes associated with Autism Spectrum Disorder","degree_awarded":"PhD","author":[{"last_name":"Dotter","first_name":"Christoph","id":"4C66542E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-9033-9096","full_name":"Dotter, Christoph"}],"file":[{"file_name":"220923_Thesis_CDotter_Final.pdf","embargo":"2023-09-19","relation":"main_file","content_type":"application/pdf","file_size":20457465,"creator":"cchlebak","date_updated":"2023-09-20T22:30:03Z","file_id":"12365","checksum":"896f4cac9adb6d3f26a6605772f4e1a3","date_created":"2023-01-24T13:15:45Z","access_level":"open_access"},{"file_name":"latex_source_CDotter_Thesis_2022.zip","relation":"source_file","content_type":"application/x-zip-compressed","file_size":22433512,"embargo_to":"open_access","creator":"cchlebak","date_updated":"2023-09-20T22:30:03Z","file_id":"12482","checksum":"ad01bb20da163be6893b7af832e58419","date_created":"2023-02-02T09:15:35Z","access_level":"closed"}],"day":"19","related_material":{"record":[{"relation":"part_of_dissertation","id":"3","status":"public"},{"relation":"part_of_dissertation","id":"11160","status":"public"}]},"citation":{"ama":"Dotter C. Transcriptional consequences of mutations in genes associated with Autism Spectrum Disorder. 2022. doi:<a href=\"https://doi.org/10.15479/at:ista:12094\">10.15479/at:ista:12094</a>","mla":"Dotter, Christoph. <i>Transcriptional Consequences of Mutations in Genes Associated with Autism Spectrum Disorder</i>. Institute of Science and Technology Austria, 2022, doi:<a href=\"https://doi.org/10.15479/at:ista:12094\">10.15479/at:ista:12094</a>.","ista":"Dotter C. 2022. Transcriptional consequences of mutations in genes associated with Autism Spectrum Disorder. Institute of Science and Technology Austria.","apa":"Dotter, C. (2022). <i>Transcriptional consequences of mutations in genes associated with Autism Spectrum Disorder</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/at:ista:12094\">https://doi.org/10.15479/at:ista:12094</a>","ieee":"C. Dotter, “Transcriptional consequences of mutations in genes associated with Autism Spectrum Disorder,” Institute of Science and Technology Austria, 2022.","chicago":"Dotter, Christoph. “Transcriptional Consequences of Mutations in Genes Associated with Autism Spectrum Disorder.” Institute of Science and Technology Austria, 2022. <a href=\"https://doi.org/10.15479/at:ista:12094\">https://doi.org/10.15479/at:ista:12094</a>.","short":"C. Dotter, Transcriptional Consequences of Mutations in Genes Associated with Autism Spectrum Disorder, Institute of Science and Technology Austria, 2022."},"alternative_title":["ISTA Thesis"],"status":"public","supervisor":[{"first_name":"Gaia","last_name":"Novarino","id":"3E57A680-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-7673-7178","full_name":"Novarino, Gaia"}],"ddc":["570"],"date_published":"2022-09-19T00:00:00Z","oa":1,"publication_status":"published","has_accepted_license":"1","_id":"12364","year":"2022","file_date_updated":"2023-09-20T22:30:03Z","date_created":"2023-01-24T13:09:57Z","page":"152","date_updated":"2023-11-16T13:10:22Z","abstract":[{"text":"Autism spectrum disorders (ASDs) are a group of neurodevelopmental disorders character\u0002ized by behavioral symptoms such as problems in social communication and interaction, as\r\nwell as repetitive, restricted behaviors and interests. These disorders show a high degree\r\nof heritability and hundreds of risk genes have been identifed using high throughput\r\nsequencing technologies. This genetic heterogeneity has hampered eforts in understanding\r\nthe pathogenesis of ASD but at the same time given rise to the concept of convergent\r\nmechanisms. Previous studies have identifed that risk genes for ASD broadly converge\r\nonto specifc functional categories with transcriptional regulation being one of the biggest\r\ngroups. In this thesis, I focus on this subgroup of genes and investigate the gene regulatory\r\nconsequences of some of them in the context of neurodevelopment.\r\nFirst, we showed that mutations in the ASD and intellectual disability risk gene Setd5 lead\r\nto perturbations of gene regulatory programs in early cell fate specifcation. In addition,\r\nadult animals display abnormal learning behavior which is mirrored at the transcriptional\r\nlevel by altered activity dependent regulation of postsynaptic gene expression. Lastly,\r\nwe link the regulatory function of Setd5 to its interaction with the Paf1 and the NCoR\r\ncomplex.\r\nSecond, by modeling the heterozygous loss of the top ASD gene CHD8 in human cerebral\r\norganoids we demonstrate profound changes in the developmental trajectories of both\r\ninhibitory and excitatory neurons using single cell RNA-sequencing. While the former\r\nwere generated earlier in CHD8+/- organoids, the generation of the latter was shifted to\r\nlater times in favor of a prolonged progenitor expansion phase and ultimately increased\r\norganoid size.\r\nFinally, by modeling heterozygous mutations for four ASD associated chromatin modifers,\r\nASH1L, KDM6B, KMT5B, and SETD5 in human cortical spheroids we show evidence of\r\nregulatory convergence across three of those genes. We observe a shift from dorsal cortical\r\nexcitatory neuron fates towards partially ventralized cell types resembling cells from the\r\nlateral ganglionic eminence. As this project is still ongoing at the time of writing, future\r\nexperiments will aim at elucidating the regulatory mechanisms underlying this shift with\r\nthe aim of linking these three ASD risk genes through biological convergence.","lang":"eng"}],"type":"dissertation","month":"09","oa_version":"Published Version"},{"doi":"10.1038/s41467-021-23123-x","quality_controlled":"1","publication_identifier":{"eissn":["2041-1723"]},"isi":1,"keyword":["General Biochemistry","Genetics and Molecular Biology"],"language":[{"iso":"eng"}],"issue":"1","project":[{"_id":"260C2330-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"ISTplus - Postdoctoral Fellowships","grant_number":"754411"},{"_id":"25444568-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"Probing the Reversibility of Autism Spectrum Disorders by Employing in vivo and in vitro Models","grant_number":"715508"},{"grant_number":"W1232-B24","call_identifier":"FWF","_id":"2548AE96-B435-11E9-9278-68D0E5697425","name":"Molecular Drug Targets"},{"_id":"05A0D778-7A3F-11EA-A408-12923DDC885E","name":"Neural stem cells in autism and epilepsy","grant_number":"F07807"},{"grant_number":"I03600","call_identifier":"FWF","_id":"265CB4D0-B435-11E9-9278-68D0E5697425","name":"Optical control of synaptic function via adhesion molecules"}],"title":"Cul3 regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development","article_number":"3058","author":[{"full_name":"Morandell, Jasmin","id":"4739D480-F248-11E8-B48F-1D18A9856A87","first_name":"Jasmin","last_name":"Morandell"},{"id":"29A8453C-F248-11E8-B48F-1D18A9856A87","full_name":"Schwarz, Lena A","last_name":"Schwarz","first_name":"Lena A"},{"first_name":"Bernadette","last_name":"Basilico","orcid":"0000-0003-1843-3173","id":"36035796-5ACA-11E9-A75E-7AF2E5697425","full_name":"Basilico, Bernadette"},{"orcid":"0000-0003-1671-393X","id":"4323B49C-F248-11E8-B48F-1D18A9856A87","full_name":"Tasciyan, Saren","last_name":"Tasciyan","first_name":"Saren"},{"last_name":"Dimchev","first_name":"Georgi A","orcid":"0000-0001-8370-6161","id":"38C393BE-F248-11E8-B48F-1D18A9856A87","full_name":"Dimchev, Georgi A"},{"full_name":"Nicolas, Armel","id":"2A103192-F248-11E8-B48F-1D18A9856A87","last_name":"Nicolas","first_name":"Armel"},{"full_name":"Sommer, Christoph M","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-1216-9105","last_name":"Sommer","first_name":"Christoph M"},{"last_name":"Kreuzinger","first_name":"Caroline","full_name":"Kreuzinger, Caroline","id":"382077BA-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Dotter","first_name":"Christoph","full_name":"Dotter, Christoph","orcid":"0000-0002-9033-9096","id":"4C66542E-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Lisa","last_name":"Knaus","full_name":"Knaus, Lisa","id":"3B2ABCF4-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Dobler, Zoe","id":"D23090A2-9057-11EA-883A-A8396FC7A38F","last_name":"Dobler","first_name":"Zoe"},{"last_name":"Cacci","first_name":"Emanuele","full_name":"Cacci, Emanuele"},{"first_name":"Florian KM","last_name":"Schur","full_name":"Schur, Florian KM","orcid":"0000-0003-4790-8078","id":"48AD8942-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Danzl","first_name":"Johann G","orcid":"0000-0001-8559-3973","id":"42EFD3B6-F248-11E8-B48F-1D18A9856A87","full_name":"Danzl, Johann G"},{"full_name":"Novarino, Gaia","orcid":"0000-0002-7673-7178","id":"3E57A680-F248-11E8-B48F-1D18A9856A87","first_name":"Gaia","last_name":"Novarino"}],"file":[{"file_name":"2021_NatureCommunications_Morandell.pdf","success":1,"file_size":9358599,"content_type":"application/pdf","relation":"main_file","creator":"kschuh","file_id":"9430","date_updated":"2021-05-28T12:39:43Z","checksum":"337e0f7959c35ec959984cacdcb472ba","date_created":"2021-05-28T12:39:43Z","access_level":"open_access"}],"day":"24","publication":"Nature Communications","ec_funded":1,"article_processing_charge":"No","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"article_type":"original","publisher":"Springer Nature","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","department":[{"_id":"GaNo"},{"_id":"JoDa"},{"_id":"FlSc"},{"_id":"MiSi"},{"_id":"LifeSc"},{"_id":"Bio"}],"ddc":["572"],"date_published":"2021-05-24T00:00:00Z","acknowledged_ssus":[{"_id":"PreCl"}],"publication_status":"published","oa":1,"has_accepted_license":"1","intvolume":"        12","related_material":{"record":[{"id":"7800","status":"public","relation":"earlier_version"},{"id":"12401","status":"public","relation":"dissertation_contains"}],"link":[{"url":"https://ist.ac.at/en/news/defective-gene-slows-down-brain-cells/","relation":"press_release"}]},"citation":{"short":"J. Morandell, L.A. Schwarz, B. Basilico, S. Tasciyan, G.A. Dimchev, A. Nicolas, C.M. Sommer, C. Kreuzinger, C. Dotter, L. Knaus, Z. Dobler, E. Cacci, F.K. Schur, J.G. Danzl, G. Novarino, Nature Communications 12 (2021).","ieee":"J. Morandell <i>et al.</i>, “Cul3 regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development,” <i>Nature Communications</i>, vol. 12, no. 1. Springer Nature, 2021.","chicago":"Morandell, Jasmin, Lena A Schwarz, Bernadette Basilico, Saren Tasciyan, Georgi A Dimchev, Armel Nicolas, Christoph M Sommer, et al. “Cul3 Regulates Cytoskeleton Protein Homeostasis and Cell Migration during a Critical Window of Brain Development.” <i>Nature Communications</i>. Springer Nature, 2021. <a href=\"https://doi.org/10.1038/s41467-021-23123-x\">https://doi.org/10.1038/s41467-021-23123-x</a>.","ista":"Morandell J, Schwarz LA, Basilico B, Tasciyan S, Dimchev GA, Nicolas A, Sommer CM, Kreuzinger C, Dotter C, Knaus L, Dobler Z, Cacci E, Schur FK, Danzl JG, Novarino G. 2021. Cul3 regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development. Nature Communications. 12(1), 3058.","mla":"Morandell, Jasmin, et al. “Cul3 Regulates Cytoskeleton Protein Homeostasis and Cell Migration during a Critical Window of Brain Development.” <i>Nature Communications</i>, vol. 12, no. 1, 3058, Springer Nature, 2021, doi:<a href=\"https://doi.org/10.1038/s41467-021-23123-x\">10.1038/s41467-021-23123-x</a>.","apa":"Morandell, J., Schwarz, L. A., Basilico, B., Tasciyan, S., Dimchev, G. A., Nicolas, A., … Novarino, G. (2021). Cul3 regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-021-23123-x\">https://doi.org/10.1038/s41467-021-23123-x</a>","ama":"Morandell J, Schwarz LA, Basilico B, et al. Cul3 regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development. <i>Nature Communications</i>. 2021;12(1). doi:<a href=\"https://doi.org/10.1038/s41467-021-23123-x\">10.1038/s41467-021-23123-x</a>"},"external_id":{"isi":["000658769900010"]},"status":"public","volume":12,"file_date_updated":"2021-05-28T12:39:43Z","date_created":"2021-05-28T11:49:46Z","date_updated":"2024-09-10T12:04:26Z","abstract":[{"lang":"eng","text":"De novo loss of function mutations in the ubiquitin ligase-encoding gene Cullin3 lead to autism spectrum disorder (ASD). In mouse, constitutive haploinsufficiency leads to motor coordination deficits as well as ASD-relevant social and cognitive impairments. However, induction of Cul3 haploinsufficiency later in life does not lead to ASD-relevant behaviors, pointing to an important role of Cul3 during a critical developmental window. Here we show that Cul3 is essential to regulate neuronal migration and, therefore, constitutive Cul3 heterozygous mutant mice display cortical lamination abnormalities. At the molecular level, we found that Cul3 controls neuronal migration by tightly regulating the amount of Plastin3 (Pls3), a previously unrecognized player of neural migration. Furthermore, we found that Pls3 cell-autonomously regulates cell migration by regulating actin cytoskeleton organization, and its levels are inversely proportional to neural migration speed. Finally, we provide evidence that cellular phenotypes associated with autism-linked gene haploinsufficiency can be rescued by transcriptional activation of the intact allele in vitro, offering a proof of concept for a potential therapeutic approach for ASDs."}],"month":"05","type":"journal_article","oa_version":"Published Version","_id":"9429","acknowledgement":"We thank A. Coll Manzano, F. Freeman, M. Ladron de Guevara, and A. Ç. Yahya for technical assistance, S. Deixler, A. Lepold, and A. Schlerka for the management of our animal colony, as well as M. Schunn and the Preclinical Facility team for technical assistance. We thank K. Heesom and her team at the University of Bristol Proteomics Facility for the proteomics sample preparation, data generation, and analysis support. We thank Y. B. Simon for kindly providing the plasmid for lentiviral labeling. Further, we thank M. Sixt for his advice regarding cell migration and the fruitful discussions. This work was supported by the ISTPlus postdoctoral fellowship (Grant Agreement No. 754411) to B.B., by the European Union’s Horizon 2020 research and innovation program (ERC) grant 715508 (REVERSEAUTISM), and by the Austrian Science Fund (FWF) to G.N. (DK W1232-B24 and SFB F7807-B) and to J.G.D (I3600-B27).","year":"2021"},{"file_date_updated":"2022-05-16T07:02:27Z","date_created":"2021-11-14T23:01:24Z","volume":12,"abstract":[{"text":"Mutations affecting mTOR or RAS signaling underlie defined syndromes (the so-called mTORopathies and RASopathies) with high risk for Autism Spectrum Disorder (ASD). These syndromes show a broad variety of somatic phenotypes including cancers, skin abnormalities, heart disease and facial dysmorphisms. Less well studied are the neuropsychiatric symptoms such as ASD. Here, we assess the relevance of these signalopathies in ASD reviewing genetic, human cell model, rodent studies and clinical trials. We conclude that signalopathies have an increased liability for ASD and that, in particular, ASD individuals with dysmorphic features and intellectual disability (ID) have a higher chance for disruptive mutations in RAS- and mTOR-related genes. Studies on rodent and human cell models confirm aberrant neuronal development as the underlying pathology. Human studies further suggest that multiple hits are necessary to induce the respective phenotypes. Recent clinical trials do only report improvements for comorbid conditions such as epilepsy or cancer but not for behavioral aspects. Animal models show that treatment during early development can rescue behavioral phenotypes. Taken together, we suggest investigating the differential roles of mTOR and RAS signaling in both human and rodent models, and to test drug treatment both during and after neuronal development in the available model systems","lang":"eng"}],"date_updated":"2023-08-14T11:46:12Z","month":"10","type":"journal_article","oa_version":"Published Version","_id":"10281","year":"2021","acknowledgement":"This review was funded by the IMI2 Initiative under the grant AIMS-2-TRIALS No 777394, by the Hessian Ministry for Science and Arts; State of Hesse Ministry for Science and Arts: LOEWE-Grant to the CePTER-Consortium (www.uni-frankfurt.de/67689811); Research (BMBF) under the grant RAISE-genic No 779282 all to AGC. This work was also supported by the European Union’s Horizon 2020 research and innovation program (ERC) grant 715508 (REVERSEAUTISM) and by the Austrian Science Fund (FWF) (DK W1232-B24) both to G.N. and both BMBF GeNeRARe 01GM1519A and CRC 1080, project B10, of the German Research Foundation (DFG) to M.J.S, respectively. We want to thank R. Waltes for her support in preparing this manuscript.","ddc":["570"],"date_published":"2021-10-30T00:00:00Z","has_accepted_license":"1","oa":1,"publication_status":"published","citation":{"chicago":"Vasic, Verica, Mattson S.O. Jones, Denise Haslinger, Lisa Knaus, Michael J. Schmeisser, Gaia Novarino, and Andreas G. Chiocchetti. “Translating the Role of Mtor-and Ras-Associated Signalopathies in Autism Spectrum Disorder: Models, Mechanisms and Treatment.” <i>Genes</i>. MDPI, 2021. <a href=\"https://doi.org/10.3390/genes12111746\">https://doi.org/10.3390/genes12111746</a>.","ieee":"V. Vasic <i>et al.</i>, “Translating the role of mtor-and ras-associated signalopathies in autism spectrum disorder: Models, mechanisms and treatment,” <i>Genes</i>, vol. 12, no. 11. MDPI, 2021.","short":"V. Vasic, M.S.O. Jones, D. Haslinger, L. Knaus, M.J. Schmeisser, G. Novarino, A.G. Chiocchetti, Genes 12 (2021).","ama":"Vasic V, Jones MSO, Haslinger D, et al. Translating the role of mtor-and ras-associated signalopathies in autism spectrum disorder: Models, mechanisms and treatment. <i>Genes</i>. 2021;12(11). doi:<a href=\"https://doi.org/10.3390/genes12111746\">10.3390/genes12111746</a>","apa":"Vasic, V., Jones, M. S. O., Haslinger, D., Knaus, L., Schmeisser, M. J., Novarino, G., &#38; Chiocchetti, A. G. (2021). Translating the role of mtor-and ras-associated signalopathies in autism spectrum disorder: Models, mechanisms and treatment. <i>Genes</i>. MDPI. <a href=\"https://doi.org/10.3390/genes12111746\">https://doi.org/10.3390/genes12111746</a>","ista":"Vasic V, Jones MSO, Haslinger D, Knaus L, Schmeisser MJ, Novarino G, Chiocchetti AG. 2021. Translating the role of mtor-and ras-associated signalopathies in autism spectrum disorder: Models, mechanisms and treatment. Genes. 12(11), 1746.","mla":"Vasic, Verica, et al. “Translating the Role of Mtor-and Ras-Associated Signalopathies in Autism Spectrum Disorder: Models, Mechanisms and Treatment.” <i>Genes</i>, vol. 12, no. 11, 1746, MDPI, 2021, doi:<a href=\"https://doi.org/10.3390/genes12111746\">10.3390/genes12111746</a>."},"intvolume":"        12","status":"public","external_id":{"isi":["000834044200002"]},"alternative_title":["Special Issue \"From Genes to Therapy in Autism Spectrum Disorder\""],"title":"Translating the role of mtor-and ras-associated signalopathies in autism spectrum disorder: Models, mechanisms and treatment","article_number":"1746","file":[{"creator":"dernst","file_size":1335308,"content_type":"application/pdf","relation":"main_file","file_name":"2021_Genes_Vasic.pdf","success":1,"access_level":"open_access","date_created":"2022-05-16T07:02:27Z","checksum":"256cb832a9c3051c7dc741f6423b8cbd","file_id":"11380","date_updated":"2022-05-16T07:02:27Z"}],"day":"30","author":[{"last_name":"Vasic","first_name":"Verica","full_name":"Vasic, Verica"},{"full_name":"Jones, Mattson S.O.","last_name":"Jones","first_name":"Mattson S.O."},{"id":"76922BDA-3D3B-11EA-90BD-A44F3DDC885E","full_name":"Haslinger, Denise","first_name":"Denise","last_name":"Haslinger"},{"last_name":"Knaus","first_name":"Lisa","id":"3B2ABCF4-F248-11E8-B48F-1D18A9856A87","full_name":"Knaus, Lisa"},{"last_name":"Schmeisser","first_name":"Michael J.","full_name":"Schmeisser, Michael J."},{"last_name":"Novarino","first_name":"Gaia","full_name":"Novarino, Gaia","orcid":"0000-0002-7673-7178","id":"3E57A680-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Chiocchetti, Andreas G.","first_name":"Andreas G.","last_name":"Chiocchetti"}],"scopus_import":"1","article_processing_charge":"No","ec_funded":1,"article_type":"original","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"publication":"Genes","department":[{"_id":"GaNo"}],"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","publisher":"MDPI","quality_controlled":"1","doi":"10.3390/genes12111746","publication_identifier":{"eissn":["2073-4425"]},"language":[{"iso":"eng"}],"issue":"11","isi":1,"project":[{"grant_number":"715508","name":"Probing the Reversibility of Autism Spectrum Disorders by Employing in vivo and in vitro Models","call_identifier":"H2020","_id":"25444568-B435-11E9-9278-68D0E5697425"},{"call_identifier":"FWF","_id":"2548AE96-B435-11E9-9278-68D0E5697425","name":"Molecular Drug Targets","grant_number":"W1232-B24"}]},{"intvolume":"        43","citation":{"chicago":"Parenti, Ilaria, Luis E Garcia Rabaneda, Hanna Schön, and Gaia Novarino. “Neurodevelopmental Disorders: From Genetics to Functional Pathways.” <i>Trends in Neurosciences</i>. Elsevier, 2020. <a href=\"https://doi.org/10.1016/j.tins.2020.05.004\">https://doi.org/10.1016/j.tins.2020.05.004</a>.","ieee":"I. Parenti, L. E. Garcia Rabaneda, H. Schön, and G. Novarino, “Neurodevelopmental disorders: From genetics to functional pathways,” <i>Trends in Neurosciences</i>, vol. 43, no. 8. Elsevier, pp. 608–621, 2020.","short":"I. Parenti, L.E. Garcia Rabaneda, H. Schön, G. Novarino, Trends in Neurosciences 43 (2020) 608–621.","ama":"Parenti I, Garcia Rabaneda LE, Schön H, Novarino G. Neurodevelopmental disorders: From genetics to functional pathways. <i>Trends in Neurosciences</i>. 2020;43(8):608-621. doi:<a href=\"https://doi.org/10.1016/j.tins.2020.05.004\">10.1016/j.tins.2020.05.004</a>","apa":"Parenti, I., Garcia Rabaneda, L. E., Schön, H., &#38; Novarino, G. (2020). Neurodevelopmental disorders: From genetics to functional pathways. <i>Trends in Neurosciences</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.tins.2020.05.004\">https://doi.org/10.1016/j.tins.2020.05.004</a>","ista":"Parenti I, Garcia Rabaneda LE, Schön H, Novarino G. 2020. Neurodevelopmental disorders: From genetics to functional pathways. Trends in Neurosciences. 43(8), 608–621.","mla":"Parenti, Ilaria, et al. “Neurodevelopmental Disorders: From Genetics to Functional Pathways.” <i>Trends in Neurosciences</i>, vol. 43, no. 8, Elsevier, 2020, pp. 608–21, doi:<a href=\"https://doi.org/10.1016/j.tins.2020.05.004\">10.1016/j.tins.2020.05.004</a>."},"status":"public","external_id":{"isi":["000553090600008"],"pmid":["32507511"]},"ddc":["570"],"date_published":"2020-08-01T00:00:00Z","oa":1,"publication_status":"published","has_accepted_license":"1","_id":"7957","acknowledgement":"We wish to thank Jasmin Morandell for generously sharing Figure 2. This work was supported by the European Research Council Starting Grant (grant 715508 ) to G.N.","year":"2020","volume":43,"file_date_updated":"2020-11-25T09:43:40Z","date_created":"2020-06-14T22:00:49Z","page":"608-621","date_updated":"2023-08-21T08:25:31Z","abstract":[{"text":"Neurodevelopmental disorders (NDDs) are a class of disorders affecting brain development and function and are characterized by wide genetic and clinical variability. In this review, we discuss the multiple factors that influence the clinical presentation of NDDs, with particular attention to gene vulnerability, mutational load, and the two-hit model. Despite the complex architecture of\r\nmutational events associated with NDDs, the various proteins involved appear to converge on common pathways, such as synaptic plasticity/function, chromatin remodelers and the mammalian target of rapamycin (mTOR) pathway. A thorough understanding of the mechanisms behind these pathways will hopefully lead to the identification of candidates that could be targeted for treatment approaches.","lang":"eng"}],"oa_version":"Published Version","month":"08","type":"journal_article","isi":1,"language":[{"iso":"eng"}],"issue":"8","project":[{"name":"Probing the Reversibility of Autism Spectrum Disorders by Employing in vivo and in vitro Models","call_identifier":"H2020","_id":"25444568-B435-11E9-9278-68D0E5697425","grant_number":"715508"}],"doi":"10.1016/j.tins.2020.05.004","quality_controlled":"1","publication_identifier":{"issn":["01662236"],"eissn":["1878108X"]},"publication":"Trends in Neurosciences","ec_funded":1,"article_processing_charge":"No","scopus_import":"1","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"article_type":"original","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","publisher":"Elsevier","pmid":1,"department":[{"_id":"GaNo"}],"title":"Neurodevelopmental disorders: From genetics to functional pathways","author":[{"last_name":"Parenti","first_name":"Ilaria","full_name":"Parenti, Ilaria","id":"D93538B0-5B71-11E9-AC62-02EBE5697425"},{"first_name":"Luis E","last_name":"Garcia Rabaneda","full_name":"Garcia Rabaneda, Luis E","id":"33D1B084-F248-11E8-B48F-1D18A9856A87"},{"id":"C8E17EDC-D7AA-11E9-B7B7-45ECE5697425","full_name":"Schön, Hanna","last_name":"Schön","first_name":"Hanna"},{"first_name":"Gaia","last_name":"Novarino","full_name":"Novarino, Gaia","id":"3E57A680-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-7673-7178"}],"file":[{"date_created":"2020-11-25T09:43:40Z","access_level":"open_access","file_id":"8805","date_updated":"2020-11-25T09:43:40Z","checksum":"67db0251b1d415ae59005f876fcf9e34","file_size":1439550,"relation":"main_file","content_type":"application/pdf","creator":"dernst","file_name":"2020_TrendsNeuroscience_Parenti.pdf","success":1}],"day":"01"}]