---
_id: '10714'
abstract:
- lang: eng
text: Ribosomal defects perturb stem cell differentiation, causing diseases called
ribosomopathies. How ribosome levels control stem cell differentiation is not
fully known. Here, we discovered three RNA helicases are required for ribosome
biogenesis and for Drosophila oogenesis. Loss of these helicases, which we named
Aramis, Athos and Porthos, lead to aberrant stabilization of p53, cell cycle arrest
and stalled GSC differentiation. Unexpectedly, Aramis is required for efficient
translation of a cohort of mRNAs containing a 5’-Terminal-Oligo-Pyrimidine (TOP)-motif,
including mRNAs that encode ribosomal proteins and a conserved p53 inhibitor,
Novel Nucleolar protein 1 (Non1). The TOP-motif co-regulates the translation of
growth-related mRNAs in mammals. As in mammals, the La-related protein co-regulates
the translation of TOP-motif containing RNAs during Drosophila oogenesis. Thus,
a previously unappreciated TOP-motif in Drosophila responds to reduced ribosome
biogenesis to co-regulate the translation of ribosomal proteins and a p53 repressor,
thus coupling ribosome biogenesis to GSC differentiation.
acknowledgement: We are grateful to all members of the Rangan and Fuchs labs for their
discussion and comments on the manuscript. We also thanks Dr. Sammons, Dr. Marlow,
Life Science Editors, for their thoughts and comments the manuscript Additionally,
we thank the Bloomington Stock Center, the Vienna Drosophila Resource Center, the
BDGP Gene Disruption Project, and Flybase for fly stocks, reagents, and other resources.
P.R. is funded by the NIH/NIGMS (R01GM111779-06 and RO1GM135628-01), G.F. is funded
by NSF MCB-2047629 and NIH RO3 AI144839, D.E.S. was funded by Marie Curie CIG 334077/IRTIM
and the Austrian Science Fund (FWF) grant ASI_FWF01_P29638S, and A.B is funded by
NIH R01GM116889 and American Cancer Society RSG-17-197-01-RMC.
article_processing_charge: No
article_type: original
author:
- first_name: Elliot T.
full_name: Martin, Elliot T.
last_name: Martin
- first_name: Patrick
full_name: Blatt, Patrick
last_name: Blatt
- first_name: Elaine
full_name: Ngyuen, Elaine
last_name: Ngyuen
- first_name: Roni
full_name: Lahr, Roni
last_name: Lahr
- first_name: Sangeetha
full_name: Selvam, Sangeetha
last_name: Selvam
- first_name: Hyun Ah M.
full_name: Yoon, Hyun Ah M.
last_name: Yoon
- first_name: Tyler
full_name: Pocchiari, Tyler
last_name: Pocchiari
- first_name: Shamsi
full_name: Emtenani, Shamsi
id: 49D32318-F248-11E8-B48F-1D18A9856A87
last_name: Emtenani
orcid: 0000-0001-6981-6938
- first_name: Daria E
full_name: Siekhaus, Daria E
id: 3D224B9E-F248-11E8-B48F-1D18A9856A87
last_name: Siekhaus
orcid: 0000-0001-8323-8353
- first_name: Andrea
full_name: Berman, Andrea
last_name: Berman
- first_name: Gabriele
full_name: Fuchs, Gabriele
last_name: Fuchs
- first_name: Prashanth
full_name: Rangan, Prashanth
last_name: Rangan
citation:
ama: Martin ET, Blatt P, Ngyuen E, et al. A translation control module coordinates
germline stem cell differentiation with ribosome biogenesis during Drosophila
oogenesis. Developmental Cell. 2022;57(7):883-900.e10. doi:10.1016/j.devcel.2022.03.005
apa: Martin, E. T., Blatt, P., Ngyuen, E., Lahr, R., Selvam, S., Yoon, H. A. M.,
… Rangan, P. (2022). A translation control module coordinates germline stem cell
differentiation with ribosome biogenesis during Drosophila oogenesis. Developmental
Cell. Elsevier. https://doi.org/10.1016/j.devcel.2022.03.005
chicago: Martin, Elliot T., Patrick Blatt, Elaine Ngyuen, Roni Lahr, Sangeetha Selvam,
Hyun Ah M. Yoon, Tyler Pocchiari, et al. “A Translation Control Module Coordinates
Germline Stem Cell Differentiation with Ribosome Biogenesis during Drosophila
Oogenesis.” Developmental Cell. Elsevier, 2022. https://doi.org/10.1016/j.devcel.2022.03.005.
ieee: E. T. Martin et al., “A translation control module coordinates germline
stem cell differentiation with ribosome biogenesis during Drosophila oogenesis,”
Developmental Cell, vol. 57, no. 7. Elsevier, p. 883–900.e10, 2022.
ista: Martin ET, Blatt P, Ngyuen E, Lahr R, Selvam S, Yoon HAM, Pocchiari T, Emtenani
S, Siekhaus DE, Berman A, Fuchs G, Rangan P. 2022. A translation control module
coordinates germline stem cell differentiation with ribosome biogenesis during
Drosophila oogenesis. Developmental Cell. 57(7), 883–900.e10.
mla: Martin, Elliot T., et al. “A Translation Control Module Coordinates Germline
Stem Cell Differentiation with Ribosome Biogenesis during Drosophila Oogenesis.”
Developmental Cell, vol. 57, no. 7, Elsevier, 2022, p. 883–900.e10, doi:10.1016/j.devcel.2022.03.005.
short: E.T. Martin, P. Blatt, E. Ngyuen, R. Lahr, S. Selvam, H.A.M. Yoon, T. Pocchiari,
S. Emtenani, D.E. Siekhaus, A. Berman, G. Fuchs, P. Rangan, Developmental Cell
57 (2022) 883–900.e10.
date_created: 2022-02-01T13:15:05Z
date_published: 2022-04-11T00:00:00Z
date_updated: 2023-08-02T14:07:13Z
day: '11'
department:
- _id: DaSi
doi: 10.1016/j.devcel.2022.03.005
ec_funded: 1
external_id:
isi:
- '000789021800005'
intvolume: ' 57'
isi: 1
issue: '7'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-nd/4.0/
main_file_link:
- open_access: '1'
url: https://doi.org/10.1101/2021.04.04.438367
month: '04'
oa: 1
oa_version: Preprint
page: 883-900.e10
project:
- _id: 2536F660-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '334077'
name: Investigating the role of transporters in invasive migration through junctions
- _id: 253B6E48-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P29638
name: Drosophila TNFa´s Funktion in Immunzellen
publication: Developmental Cell
publication_identifier:
eissn:
- 1878-1551
issn:
- 1534-5807
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: A translation control module coordinates germline stem cell differentiation
with ribosome biogenesis during Drosophila oogenesis
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 57
year: '2022'
...
---
_id: '10918'
abstract:
- lang: eng
text: Cellular metabolism must adapt to changing demands to enable homeostasis.
During immune responses or cancer metastasis, cells leading migration into challenging
environments require an energy boost, but what controls this capacity is unclear.
Here, we study a previously uncharacterized nuclear protein, Atossa (encoded by
CG9005), which supports macrophage invasion into the germband of Drosophila by
controlling cellular metabolism. First, nuclear Atossa increases mRNA levels of
Porthos, a DEAD-box protein, and of two metabolic enzymes, lysine-α-ketoglutarate
reductase (LKR/SDH) and NADPH glyoxylate reductase (GR/HPR), thus enhancing mitochondrial
bioenergetics. Then Porthos supports ribosome assembly and thereby raises the
translational efficiency of a subset of mRNAs, including those affecting mitochondrial
functions, the electron transport chain, and metabolism. Mitochondrial respiration
measurements, metabolomics, and live imaging indicate that Atossa and Porthos
power up OxPhos and energy production to promote the forging of a path into tissues
by leading macrophages. Since many crucial physiological responses require increases
in mitochondrial energy output, this previously undescribed genetic program may
modulate a wide range of cellular behaviors.
acknowledged_ssus:
- _id: Bio
acknowledgement: "We thank the DGRC (NIH grant 2P40OD010949-10A1) for plasmids, the
BDSC (NIH grant P40OD018537) and the VDRC for fly stocks, FlyBase for essential
genomic information, the BDGP in situ database for data (Tomancak et al, 2007),
the IST Austria Bioimaging facility for support, the VBC Core Facilities for RNA
sequencing and analysis, and C. Guet, C. Navarro, C. Desplan, T. Lecuit, I. Miguel-Aliaga,
and Siekhaus group members for comments on the manuscript. The VBCF Metabolomics
Facility is funded by the City of Vienna through the Vienna Business Agency. This
work was supported by the Marie Curie CIG 334077/IRTIM (DES), Austrian Science Fund
(FWF) Lise Meitner Fellowship M2379-B28 (MA and DES), Austrian Science Fund (FWF)
grant ASI_FWF01_P29638S (DES), NIH/NIGMS (R01GM111779-06 (PR), RO1GM135628-01 (PR),
European Research Council (ERC) grant no. 677006 “CMIL” (AB), and Natural Sciences
and Engineering Research Council of Canada\r\n(RGPIN-2019-06766) (TRH). "
article_number: e109049
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Shamsi
full_name: Emtenani, Shamsi
id: 49D32318-F248-11E8-B48F-1D18A9856A87
last_name: Emtenani
orcid: 0000-0001-6981-6938
- first_name: Elliot T
full_name: Martin, Elliot T
last_name: Martin
- first_name: Attila
full_name: György, Attila
id: 3BCEDBE0-F248-11E8-B48F-1D18A9856A87
last_name: György
orcid: 0000-0002-1819-198X
- first_name: Julia
full_name: Bicher, Julia
id: 3CCBB46E-F248-11E8-B48F-1D18A9856A87
last_name: Bicher
- first_name: Jakob-Wendelin
full_name: Genger, Jakob-Wendelin
last_name: Genger
- first_name: Thomas
full_name: Köcher, Thomas
last_name: Köcher
- first_name: Maria
full_name: Akhmanova, Maria
id: 3425EC26-F248-11E8-B48F-1D18A9856A87
last_name: Akhmanova
orcid: 0000-0003-1522-3162
- first_name: Mariana
full_name: Pereira Guarda, Mariana
id: 6de81d9d-e2f2-11eb-945a-af8bc2a60b26
last_name: Pereira Guarda
- first_name: Marko
full_name: Roblek, Marko
id: 3047D808-F248-11E8-B48F-1D18A9856A87
last_name: Roblek
orcid: 0000-0001-9588-1389
- first_name: Andreas
full_name: Bergthaler, Andreas
last_name: Bergthaler
- first_name: Thomas R
full_name: Hurd, Thomas R
last_name: Hurd
- first_name: Prashanth
full_name: Rangan, Prashanth
last_name: Rangan
- first_name: Daria E
full_name: Siekhaus, Daria E
id: 3D224B9E-F248-11E8-B48F-1D18A9856A87
last_name: Siekhaus
orcid: 0000-0001-8323-8353
citation:
ama: Emtenani S, Martin ET, György A, et al. Macrophage mitochondrial bioenergetics
and tissue invasion are boosted by an Atossa-Porthos axis in Drosophila. The
Embo Journal. 2022;41. doi:10.15252/embj.2021109049
apa: Emtenani, S., Martin, E. T., György, A., Bicher, J., Genger, J.-W., Köcher,
T., … Siekhaus, D. E. (2022). Macrophage mitochondrial bioenergetics and tissue
invasion are boosted by an Atossa-Porthos axis in Drosophila. The Embo Journal.
Embo Press. https://doi.org/10.15252/embj.2021109049
chicago: Emtenani, Shamsi, Elliot T Martin, Attila György, Julia Bicher, Jakob-Wendelin
Genger, Thomas Köcher, Maria Akhmanova, et al. “Macrophage Mitochondrial Bioenergetics
and Tissue Invasion Are Boosted by an Atossa-Porthos Axis in Drosophila.” The
Embo Journal. Embo Press, 2022. https://doi.org/10.15252/embj.2021109049.
ieee: S. Emtenani et al., “Macrophage mitochondrial bioenergetics and tissue
invasion are boosted by an Atossa-Porthos axis in Drosophila,” The Embo Journal,
vol. 41. Embo Press, 2022.
ista: Emtenani S, Martin ET, György A, Bicher J, Genger J-W, Köcher T, Akhmanova
M, Pereira Guarda M, Roblek M, Bergthaler A, Hurd TR, Rangan P, Siekhaus DE. 2022.
Macrophage mitochondrial bioenergetics and tissue invasion are boosted by an Atossa-Porthos
axis in Drosophila. The Embo Journal. 41, e109049.
mla: Emtenani, Shamsi, et al. “Macrophage Mitochondrial Bioenergetics and Tissue
Invasion Are Boosted by an Atossa-Porthos Axis in Drosophila.” The Embo Journal,
vol. 41, e109049, Embo Press, 2022, doi:10.15252/embj.2021109049.
short: S. Emtenani, E.T. Martin, A. György, J. Bicher, J.-W. Genger, T. Köcher,
M. Akhmanova, M. Pereira Guarda, M. Roblek, A. Bergthaler, T.R. Hurd, P. Rangan,
D.E. Siekhaus, The Embo Journal 41 (2022).
date_created: 2022-03-24T13:23:09Z
date_published: 2022-03-23T00:00:00Z
date_updated: 2023-08-03T06:13:14Z
day: '23'
ddc:
- '570'
department:
- _id: DaSi
- _id: LoSw
doi: 10.15252/embj.2021109049
ec_funded: 1
external_id:
isi:
- '000771957000001'
file:
- access_level: open_access
checksum: dba48580fe0fefaa4c63078d1d2a35df
content_type: application/pdf
creator: siekhaus
date_created: 2022-03-24T13:22:41Z
date_updated: 2022-03-24T13:22:41Z
file_id: '10919'
file_name: Macrophage mitochondrial bioenergetics and tissue invasion are boosted
by an Atossa-Porthos axis in Drosopila.pdf
file_size: 4344585
relation: main_file
file_date_updated: 2022-03-24T13:22:41Z
has_accepted_license: '1'
intvolume: ' 41'
isi: 1
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '03'
oa: 1
oa_version: Published Version
project:
- _id: 2536F660-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '334077'
name: Investigating the role of transporters in invasive migration through junctions
- _id: 264CBBAC-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: M02379
name: Modeling epithelial tissue mechanics during cell invasion
- _id: 253B6E48-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P29638
name: Drosophila TNFa´s Funktion in Immunzellen
publication: The Embo Journal
publication_identifier:
eissn:
- 1460-2075
publication_status: published
publisher: Embo Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: Macrophage mitochondrial bioenergetics and tissue invasion are boosted by an
Atossa-Porthos axis in Drosophila
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 41
year: '2022'
...
---
_id: '10614'
abstract:
- lang: eng
text: 'The infiltration of immune cells into tissues underlies the establishment
of tissue-resident macrophages and responses to infections and tumors. Yet the
mechanisms immune cells utilize to negotiate tissue barriers in living organisms
are not well understood, and a role for cortical actin has not been examined.
Here, we find that the tissue invasion of Drosophila macrophages, also known as
plasmatocytes or hemocytes, utilizes enhanced cortical F-actin levels stimulated
by the Drosophila member of the fos proto oncogene transcription factor family
(Dfos, Kayak). RNA sequencing analysis and live imaging show that Dfos enhances
F-actin levels around the entire macrophage surface by increasing mRNA levels
of the membrane spanning molecular scaffold tetraspanin TM4SF, and the actin cross-linking
filamin Cheerio, which are themselves required for invasion. Both the filamin
and the tetraspanin enhance the cortical activity of Rho1 and the formin Diaphanous
and thus the assembly of cortical actin, which is a critical function since expressing
a dominant active form of Diaphanous can rescue the Dfos macrophage invasion defect.
In vivo imaging shows that Dfos enhances the efficiency of the initial phases
of macrophage tissue entry. Genetic evidence argues that this Dfos-induced program
in macrophages counteracts the constraint produced by the tension of surrounding
tissues and buffers the properties of the macrophage nucleus from affecting tissue
entry. We thus identify strengthening the cortical actin cytoskeleton through
Dfos as a key process allowing efficient forward movement of an immune cell into
surrounding tissues. '
acknowledged_ssus:
- _id: LifeSc
acknowledgement: 'We thank the following for their contributions: Plasmids were supplied
by the Drosophila Genomics Resource Center (NIH 2P40OD010949-10A1); fly stocks were
provided by K. Brueckner, B. Stramer, M. Uhlirova, O. Schuldiner, the Bloomington
Drosophila Stock Center (NIH P40OD018537) and the Vienna Drosophila Resource Center,
FlyBase for essential genomic information, and the BDGP in situ database for data.
For antibodies, we thank the Developmental Studies Hybridoma Bank, which was created
by the Eunice Kennedy Shriver National Institute of Child Health and Human Development
of the NIH and is maintained at the University of Iowa, as well as J. Zeitlinger
for her generous gift of Dfos antibody. We thank the Vienna BioCenter Core Facilities
for RNA sequencing and analysis and the Life Scientific Service Units at IST Austria
for technical support and assistance with microscopy and FACS analysis. We thank
C. P. Heisenberg, P. Martin, M. Sixt, and Siekhaus group members for discussions
and T. Hurd, A. Ratheesh, and P. Rangan for comments on the manuscript.'
article_processing_charge: No
article_type: original
author:
- first_name: Vera
full_name: Belyaeva, Vera
id: 47F080FE-F248-11E8-B48F-1D18A9856A87
last_name: Belyaeva
- first_name: Stephanie
full_name: Wachner, Stephanie
id: 2A95E7B0-F248-11E8-B48F-1D18A9856A87
last_name: Wachner
- first_name: Attila
full_name: György, Attila
id: 3BCEDBE0-F248-11E8-B48F-1D18A9856A87
last_name: György
orcid: 0000-0002-1819-198X
- first_name: Shamsi
full_name: Emtenani, Shamsi
id: 49D32318-F248-11E8-B48F-1D18A9856A87
last_name: Emtenani
orcid: 0000-0001-6981-6938
- first_name: Igor
full_name: Gridchyn, Igor
id: 4B60654C-F248-11E8-B48F-1D18A9856A87
last_name: Gridchyn
orcid: 0000-0002-1807-1929
- first_name: Maria
full_name: Akhmanova, Maria
id: 3425EC26-F248-11E8-B48F-1D18A9856A87
last_name: Akhmanova
orcid: 0000-0003-1522-3162
- first_name: M
full_name: Linder, M
last_name: Linder
- first_name: Marko
full_name: Roblek, Marko
id: 3047D808-F248-11E8-B48F-1D18A9856A87
last_name: Roblek
orcid: 0000-0001-9588-1389
- first_name: M
full_name: Sibilia, M
last_name: Sibilia
- first_name: Daria E
full_name: Siekhaus, Daria E
id: 3D224B9E-F248-11E8-B48F-1D18A9856A87
last_name: Siekhaus
orcid: 0000-0001-8323-8353
citation:
ama: Belyaeva V, Wachner S, György A, et al. Fos regulates macrophage infiltration
against surrounding tissue resistance by a cortical actin-based mechanism in Drosophila.
PLoS Biology. 2022;20(1):e3001494. doi:10.1371/journal.pbio.3001494
apa: Belyaeva, V., Wachner, S., György, A., Emtenani, S., Gridchyn, I., Akhmanova,
M., … Siekhaus, D. E. (2022). Fos regulates macrophage infiltration against surrounding
tissue resistance by a cortical actin-based mechanism in Drosophila. PLoS Biology.
Public Library of Science. https://doi.org/10.1371/journal.pbio.3001494
chicago: Belyaeva, Vera, Stephanie Wachner, Attila György, Shamsi Emtenani, Igor
Gridchyn, Maria Akhmanova, M Linder, Marko Roblek, M Sibilia, and Daria E Siekhaus.
“Fos Regulates Macrophage Infiltration against Surrounding Tissue Resistance by
a Cortical Actin-Based Mechanism in Drosophila.” PLoS Biology. Public Library
of Science, 2022. https://doi.org/10.1371/journal.pbio.3001494.
ieee: V. Belyaeva et al., “Fos regulates macrophage infiltration against
surrounding tissue resistance by a cortical actin-based mechanism in Drosophila,”
PLoS Biology, vol. 20, no. 1. Public Library of Science, p. e3001494, 2022.
ista: Belyaeva V, Wachner S, György A, Emtenani S, Gridchyn I, Akhmanova M, Linder
M, Roblek M, Sibilia M, Siekhaus DE. 2022. Fos regulates macrophage infiltration
against surrounding tissue resistance by a cortical actin-based mechanism in Drosophila.
PLoS Biology. 20(1), e3001494.
mla: Belyaeva, Vera, et al. “Fos Regulates Macrophage Infiltration against Surrounding
Tissue Resistance by a Cortical Actin-Based Mechanism in Drosophila.” PLoS
Biology, vol. 20, no. 1, Public Library of Science, 2022, p. e3001494, doi:10.1371/journal.pbio.3001494.
short: V. Belyaeva, S. Wachner, A. György, S. Emtenani, I. Gridchyn, M. Akhmanova,
M. Linder, M. Roblek, M. Sibilia, D.E. Siekhaus, PLoS Biology 20 (2022) e3001494.
date_created: 2022-01-12T10:18:17Z
date_published: 2022-01-06T00:00:00Z
date_updated: 2024-03-25T23:30:15Z
day: '06'
ddc:
- '570'
department:
- _id: DaSi
- _id: JoCs
doi: 10.1371/journal.pbio.3001494
ec_funded: 1
external_id:
isi:
- '000971223700001'
pmid:
- '34990456'
file:
- access_level: open_access
checksum: f454212a5522a7818ba4b2892315c478
content_type: application/pdf
creator: cchlebak
date_created: 2022-01-12T13:50:04Z
date_updated: 2022-01-12T13:50:04Z
file_id: '10615'
file_name: 2022_PLOSBio_Belyaeva.pdf
file_size: 5426932
relation: main_file
success: 1
file_date_updated: 2022-01-12T13:50:04Z
has_accepted_license: '1'
intvolume: ' 20'
isi: 1
issue: '1'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
page: e3001494
pmid: 1
project:
- _id: 253B6E48-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P29638
name: Drosophila TNFa´s Funktion in Immunzellen
- _id: 26199CA4-B435-11E9-9278-68D0E5697425
grant_number: '24800'
name: Tissue barrier penetration is crucial for immunity and metastasis
- _id: 2536F660-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '334077'
name: Investigating the role of transporters in invasive migration through junctions
publication: PLoS Biology
publication_identifier:
eissn:
- 1545-7885
issn:
- 1544-9173
publication_status: published
publisher: Public Library of Science
quality_controlled: '1'
related_material:
link:
- relation: earlier_version
url: https://www.biorxiv.org/content/10.1101/2020.09.18.301481
- description: News on the ISTA Website
relation: press_release
url: https://ista.ac.at/en/news/resisting-the-pressure/
record:
- id: '8557'
relation: earlier_version
status: public
- id: '11193'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Fos regulates macrophage infiltration against surrounding tissue resistance
by a cortical actin-based mechanism in Drosophila
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 20
year: '2022'
...
---
_id: '8557'
abstract:
- lang: eng
text: The infiltration of immune cells into tissues underlies the establishment
of tissue resident macrophages, and responses to infections and tumors. Yet the
mechanisms immune cells utilize to negotiate tissue barriers in living organisms
are not well understood, and a role for cortical actin has not been examined.
Here we find that the tissue invasion of Drosophila macrophages, also known as
plasmatocytes or hemocytes, utilizes enhanced cortical F-actin levels stimulated
by the Drosophila member of the fos proto oncogene transcription factor family
(Dfos, Kayak). RNA sequencing analysis and live imaging show that Dfos enhances
F-actin levels around the entire macrophage surface by increasing mRNA levels
of the membrane spanning molecular scaffold tetraspanin TM4SF, and the actin cross-linking
filamin Cheerio which are themselves required for invasion. Cortical F-actin levels
are critical as expressing a dominant active form of Diaphanous, a actin polymerizing
Formin, can rescue the Dfos Dominant Negative macrophage invasion defect. In vivo
imaging shows that Dfos is required to enhance the efficiency of the initial phases
of macrophage tissue entry. Genetic evidence argues that this Dfos-induced program
in macrophages counteracts the constraint produced by the tension of surrounding
tissues and buffers the mechanical properties of the macrophage nucleus from affecting
tissue entry. We thus identify tuning the cortical actin cytoskeleton through
Dfos as a key process allowing efficient forward movement of an immune cell into
surrounding tissues.
acknowledged_ssus:
- _id: LifeSc
acknowledgement: 'We thank the following for their contributions: The Drosophila Genomics
Resource Center supported by NIH grant 2P40OD010949-10A1 for plasmids, K. Brueckner.
B. Stramer, M. Uhlirova, O. Schuldiner, the Bloomington Drosophila Stock Center
supported by NIH grant P40OD018537 and the Vienna Drosophila Resource Center for
fly stocks, FlyBase (Thurmond et al., 2019) for essential genomic information, and
the BDGP in situ database for data (Tomancak et al., 2002, 2007). For antibodies,
we thank the Developmental Studies Hybridoma Bank, which was created by the Eunice
Kennedy Shriver National Institute of Child Health and Human Development of the
NIH, and is maintained at the University of Iowa, as well as J. Zeitlinger for her
generous gift of Dfos antibody. We thank the Vienna BioCenter Core Facilities for
RNA sequencing and analysis and the Life Scientific Service Units at IST Austria
for technical support and assistance with microscopy and FACS analysis. We thank
C.P. Heisenberg, P. Martin, M. Sixt and Siekhaus group members for discussions and
T.Hurd, A. Ratheesh and P. Rangan for comments on the manuscript. A.G. was supported
by the Austrian Science Fund (FWF) grant DASI_FWF01_P29638S, D.E.S. by Marie Curie
CIG 334077/IRTIM. M.S. is supported by the FWF, PhD program W1212 915 and the European
Research Council (ERC) Advanced grant (ERC-2015-AdG TNT-Tumors 694883). S.W. is
supported by an OEAW, DOC fellowship.'
article_processing_charge: No
author:
- first_name: Vera
full_name: Belyaeva, Vera
id: 47F080FE-F248-11E8-B48F-1D18A9856A87
last_name: Belyaeva
- first_name: Stephanie
full_name: Wachner, Stephanie
id: 2A95E7B0-F248-11E8-B48F-1D18A9856A87
last_name: Wachner
- first_name: Igor
full_name: Gridchyn, Igor
id: 4B60654C-F248-11E8-B48F-1D18A9856A87
last_name: Gridchyn
orcid: 0000-0002-1807-1929
- first_name: Markus
full_name: Linder, Markus
last_name: Linder
- first_name: Shamsi
full_name: Emtenani, Shamsi
id: 49D32318-F248-11E8-B48F-1D18A9856A87
last_name: Emtenani
orcid: 0000-0001-6981-6938
- first_name: Attila
full_name: György, Attila
id: 3BCEDBE0-F248-11E8-B48F-1D18A9856A87
last_name: György
orcid: 0000-0002-1819-198X
- first_name: Maria
full_name: Sibilia, Maria
last_name: Sibilia
- first_name: Daria E
full_name: Siekhaus, Daria E
id: 3D224B9E-F248-11E8-B48F-1D18A9856A87
last_name: Siekhaus
orcid: 0000-0001-8323-8353
citation:
ama: Belyaeva V, Wachner S, Gridchyn I, et al. Cortical actin properties controlled
by Drosophila Fos aid macrophage infiltration against surrounding tissue resistance.
bioRxiv. doi:10.1101/2020.09.18.301481
apa: Belyaeva, V., Wachner, S., Gridchyn, I., Linder, M., Emtenani, S., György,
A., … Siekhaus, D. E. (n.d.). Cortical actin properties controlled by Drosophila
Fos aid macrophage infiltration against surrounding tissue resistance. bioRxiv.
https://doi.org/10.1101/2020.09.18.301481
chicago: Belyaeva, Vera, Stephanie Wachner, Igor Gridchyn, Markus Linder, Shamsi
Emtenani, Attila György, Maria Sibilia, and Daria E Siekhaus. “Cortical Actin
Properties Controlled by Drosophila Fos Aid Macrophage Infiltration against Surrounding
Tissue Resistance.” BioRxiv, n.d. https://doi.org/10.1101/2020.09.18.301481.
ieee: V. Belyaeva et al., “Cortical actin properties controlled by Drosophila
Fos aid macrophage infiltration against surrounding tissue resistance,” bioRxiv.
.
ista: Belyaeva V, Wachner S, Gridchyn I, Linder M, Emtenani S, György A, Sibilia
M, Siekhaus DE. Cortical actin properties controlled by Drosophila Fos aid macrophage
infiltration against surrounding tissue resistance. bioRxiv, 10.1101/2020.09.18.301481.
mla: Belyaeva, Vera, et al. “Cortical Actin Properties Controlled by Drosophila
Fos Aid Macrophage Infiltration against Surrounding Tissue Resistance.” BioRxiv,
doi:10.1101/2020.09.18.301481.
short: V. Belyaeva, S. Wachner, I. Gridchyn, M. Linder, S. Emtenani, A. György,
M. Sibilia, D.E. Siekhaus, BioRxiv (n.d.).
date_created: 2020-09-23T09:36:47Z
date_published: 2020-09-18T00:00:00Z
date_updated: 2024-03-25T23:30:12Z
day: '18'
department:
- _id: DaSi
- _id: JoCs
doi: 10.1101/2020.09.18.301481
ec_funded: 1
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1101/2020.09.18.301481
month: '09'
oa: 1
oa_version: Preprint
project:
- _id: 253B6E48-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P29638
name: Drosophila TNFa´s Funktion in Immunzellen
- _id: 2536F660-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '334077'
name: Investigating the role of transporters in invasive migration through junctions
- _id: 26199CA4-B435-11E9-9278-68D0E5697425
grant_number: '24800'
name: Tissue barrier penetration is crucial for immunity and metastasis
publication: bioRxiv
publication_status: submitted
related_material:
record:
- id: '10614'
relation: later_version
status: public
- id: '8983'
relation: dissertation_contains
status: public
status: public
title: Cortical actin properties controlled by Drosophila Fos aid macrophage infiltration
against surrounding tissue resistance
type: preprint
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2020'
...
---
_id: '8'
abstract:
- lang: eng
text: Despite their different origins, Drosophila glia and hemocytes are related
cell populations that provide an immune function. Drosophila hemocytes patrol
the body cavity and act as macrophages outside the nervous system whereas glia
originate from the neuroepithelium and provide the scavenger population of the
nervous system. Drosophila glia are hence the functional orthologs of vertebrate
microglia, even though the latter are cells of immune origin that subsequently
move into the brain during development. Interestingly, the Drosophila immune cells
within (glia) and outside the nervous system (hemocytes) require the same transcription
factor Glide/Gcm for their development. This raises the issue of how do glia specifically
differentiate in the nervous system and hemocytes in the procephalic mesoderm.
The Repo homeodomain transcription factor and pan-glial direct target of Glide/Gcm
is known to ensure glial terminal differentiation. Here we show that Repo also
takes center stage in the process that discriminates between glia and hemocytes.
First, Repo expression is repressed in the hemocyte anlagen by mesoderm-specific
factors. Second, Repo ectopic activation in the procephalic mesoderm is sufficient
to repress the expression of hemocyte-specific genes. Third, the lack of Repo
triggers the expression of hemocyte markers in glia. Thus, a complex network of
tissue-specific cues biases the potential of Glide/Gcm. These data allow us to
revise the concept of fate determinants and help us understand the bases of cell
specification. Both sexes were analyzed.SIGNIFICANCE STATEMENTDistinct cell types
often require the same pioneer transcription factor, raising the issue of how
does one factor trigger different fates. In Drosophila, glia and hemocytes provide
a scavenger activity within and outside the nervous system, respectively. While
they both require the Glide/Gcm transcription factor, glia originate from the
ectoderm, hemocytes from the mesoderm. Here we show that tissue-specific factors
inhibit the gliogenic potential of Glide/Gcm in the mesoderm by repressing the
expression of the homeodomain protein Repo, a major glial-specific target of Glide/Gcm.
Repo expression in turn inhibits the expression of hemocyte-specific genes in
the nervous system. These cell-specific networks secure the establishment of the
glial fate only in the nervous system and allow cell diversification.
acknowledgement: This work was supported by INSERM, CNRS, UDS, Ligue Régionale contre
le Cancer, Hôpital de Strasbourg, Association pour la Recherche sur le Cancer (ARC)
and Agence Nationale de la Recherche (ANR) grants. P.B.C. was funded by the ANR
and by the ARSEP (Fondation pour l'Aide à la Recherche sur la Sclérose en Plaques),
and G.T. by governmental and ARC fellowships. This work was also supported by grants
from the Ataxia UK (2491) and the NC3R (NC/L000199/1) awarded to M.F. The Institut
de Génétique et de Biologie Moléculaire et Cellulaire was also supported by a French
state fund through the ANR labex. D.E.S. was funded by Marie Curie Grant CIG 334077/IRTIM.
We thank B. Altenhein, K. Brückner, M. Crozatier, L. Waltzer, M. Logan, E. Kurant,
R. Reuter, E. Kurucz, J.L Dimarcq, J. Hoffmann, C. Goodman, the DHSB, and the BDSC
for reagents and flies. We also thank all of the laboratory members for comments
on the manuscript; C. Diebold, C. Delaporte, M. Pezze, the fly, and imaging and
antibody facilities for technical assistance; and D. Dembele for help with statistics.
In addition, we thank Alison Brewer for help with Luciferase assays.
article_processing_charge: No
article_type: original
author:
- first_name: Guillaume
full_name: Trébuchet, Guillaume
last_name: Trébuchet
- first_name: Pierre B
full_name: Cattenoz, Pierre B
last_name: Cattenoz
- first_name: János
full_name: Zsámboki, János
last_name: Zsámboki
- first_name: David
full_name: Mazaud, David
last_name: Mazaud
- first_name: Daria E
full_name: Siekhaus, Daria E
id: 3D224B9E-F248-11E8-B48F-1D18A9856A87
last_name: Siekhaus
orcid: 0000-0001-8323-8353
- first_name: Manolis
full_name: Fanto, Manolis
last_name: Fanto
- first_name: Angela
full_name: Giangrande, Angela
last_name: Giangrande
citation:
ama: Trébuchet G, Cattenoz PB, Zsámboki J, et al. The Repo homeodomain transcription
factor suppresses hematopoiesis in Drosophila and preserves the glial fate. Journal
of Neuroscience. 2019;39(2):238-255. doi:10.1523/JNEUROSCI.1059-18.2018
apa: Trébuchet, G., Cattenoz, P. B., Zsámboki, J., Mazaud, D., Siekhaus, D. E.,
Fanto, M., & Giangrande, A. (2019). The Repo homeodomain transcription factor
suppresses hematopoiesis in Drosophila and preserves the glial fate. Journal
of Neuroscience. Society for Neuroscience. https://doi.org/10.1523/JNEUROSCI.1059-18.2018
chicago: Trébuchet, Guillaume, Pierre B Cattenoz, János Zsámboki, David Mazaud,
Daria E Siekhaus, Manolis Fanto, and Angela Giangrande. “The Repo Homeodomain
Transcription Factor Suppresses Hematopoiesis in Drosophila and Preserves the
Glial Fate.” Journal of Neuroscience. Society for Neuroscience, 2019. https://doi.org/10.1523/JNEUROSCI.1059-18.2018.
ieee: G. Trébuchet et al., “The Repo homeodomain transcription factor suppresses
hematopoiesis in Drosophila and preserves the glial fate,” Journal of Neuroscience,
vol. 39, no. 2. Society for Neuroscience, pp. 238–255, 2019.
ista: Trébuchet G, Cattenoz PB, Zsámboki J, Mazaud D, Siekhaus DE, Fanto M, Giangrande
A. 2019. The Repo homeodomain transcription factor suppresses hematopoiesis in
Drosophila and preserves the glial fate. Journal of Neuroscience. 39(2), 238–255.
mla: Trébuchet, Guillaume, et al. “The Repo Homeodomain Transcription Factor Suppresses
Hematopoiesis in Drosophila and Preserves the Glial Fate.” Journal of Neuroscience,
vol. 39, no. 2, Society for Neuroscience, 2019, pp. 238–55, doi:10.1523/JNEUROSCI.1059-18.2018.
short: G. Trébuchet, P.B. Cattenoz, J. Zsámboki, D. Mazaud, D.E. Siekhaus, M. Fanto,
A. Giangrande, Journal of Neuroscience 39 (2019) 238–255.
date_created: 2018-12-11T11:44:07Z
date_published: 2019-01-09T00:00:00Z
date_updated: 2023-09-19T10:10:55Z
day: '09'
ddc:
- '570'
department:
- _id: DaSi
doi: 10.1523/JNEUROSCI.1059-18.2018
ec_funded: 1
external_id:
isi:
- '000455189900006'
pmid:
- '30504274'
file:
- access_level: open_access
checksum: 8f6925eb4cd1e8747d8ea25929c68de6
content_type: application/pdf
creator: dernst
date_created: 2020-10-02T09:33:28Z
date_updated: 2020-10-02T09:33:28Z
file_id: '8596'
file_name: 2019_JournNeuroscience_Trebuchet.pdf
file_size: 9455414
relation: main_file
success: 1
file_date_updated: 2020-10-02T09:33:28Z
has_accepted_license: '1'
intvolume: ' 39'
isi: 1
issue: '2'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
page: 238-255
pmid: 1
project:
- _id: 2536F660-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '334077'
name: Investigating the role of transporters in invasive migration through junctions
publication: Journal of Neuroscience
publication_status: published
publisher: Society for Neuroscience
publist_id: '8048'
quality_controlled: '1'
scopus_import: '1'
status: public
title: The Repo homeodomain transcription factor suppresses hematopoiesis in Drosophila
and preserves the glial fate
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 39
year: '2019'
...
---
_id: '6187'
abstract:
- lang: eng
text: Aberrant display of the truncated core1 O-glycan T-antigen is a common feature
of human cancer cells that correlates with metastasis. Here we show that T-antigen
in Drosophila melanogaster macrophages is involved in their developmentally programmed
tissue invasion. Higher macrophage T-antigen levels require an atypical major
facilitator superfamily (MFS) member that we named Minerva which enables macrophage
dissemination and invasion. We characterize for the first time the T and Tn glycoform
O-glycoproteome of the Drosophila melanogaster embryo, and determine that Minerva
increases the presence of T-antigen on proteins in pathways previously linked
to cancer, most strongly on the sulfhydryl oxidase Qsox1 which we show is required
for macrophage tissue entry. Minerva’s vertebrate ortholog, MFSD1, rescues the
minerva mutant’s migration and T-antigen glycosylation defects. We thus identify
a key conserved regulator that orchestrates O-glycosylation on a protein subset
to activate a program governing migration steps important for both development
and cancer metastasis.
acknowledged_ssus:
- _id: LifeSc
article_number: e41801
article_processing_charge: No
author:
- first_name: Katarina
full_name: Valosková, Katarina
id: 46F146FC-F248-11E8-B48F-1D18A9856A87
last_name: Valosková
- first_name: Julia
full_name: Biebl, Julia
id: 3CCBB46E-F248-11E8-B48F-1D18A9856A87
last_name: Biebl
- first_name: Marko
full_name: Roblek, Marko
id: 3047D808-F248-11E8-B48F-1D18A9856A87
last_name: Roblek
orcid: 0000-0001-9588-1389
- first_name: Shamsi
full_name: Emtenani, Shamsi
id: 49D32318-F248-11E8-B48F-1D18A9856A87
last_name: Emtenani
orcid: 0000-0001-6981-6938
- first_name: Attila
full_name: György, Attila
id: 3BCEDBE0-F248-11E8-B48F-1D18A9856A87
last_name: György
orcid: 0000-0002-1819-198X
- first_name: Michaela
full_name: Misova, Michaela
id: 495A3C32-F248-11E8-B48F-1D18A9856A87
last_name: Misova
orcid: 0000-0003-2427-6856
- first_name: Aparna
full_name: Ratheesh, Aparna
id: 2F064CFE-F248-11E8-B48F-1D18A9856A87
last_name: Ratheesh
orcid: 0000-0001-7190-0776
- first_name: Patricia
full_name: Rodrigues, Patricia
id: 2CE4065A-F248-11E8-B48F-1D18A9856A87
last_name: Rodrigues
- first_name: Katerina
full_name: Shkarina, Katerina
last_name: Shkarina
- first_name: Ida Signe Bohse
full_name: Larsen, Ida Signe Bohse
last_name: Larsen
- first_name: Sergey Y
full_name: Vakhrushev, Sergey Y
last_name: Vakhrushev
- first_name: Henrik
full_name: Clausen, Henrik
last_name: Clausen
- first_name: Daria E
full_name: Siekhaus, Daria E
id: 3D224B9E-F248-11E8-B48F-1D18A9856A87
last_name: Siekhaus
orcid: 0000-0001-8323-8353
citation:
ama: Valosková K, Bicher J, Roblek M, et al. A conserved major facilitator superfamily
member orchestrates a subset of O-glycosylation to aid macrophage tissue invasion.
eLife. 2019;8. doi:10.7554/elife.41801
apa: Valosková, K., Bicher, J., Roblek, M., Emtenani, S., György, A., Misova, M.,
… Siekhaus, D. E. (2019). A conserved major facilitator superfamily member orchestrates
a subset of O-glycosylation to aid macrophage tissue invasion. ELife. eLife
Sciences Publications. https://doi.org/10.7554/elife.41801
chicago: Valosková, Katarina, Julia Bicher, Marko Roblek, Shamsi Emtenani, Attila
György, Michaela Misova, Aparna Ratheesh, et al. “A Conserved Major Facilitator
Superfamily Member Orchestrates a Subset of O-Glycosylation to Aid Macrophage
Tissue Invasion.” ELife. eLife Sciences Publications, 2019. https://doi.org/10.7554/elife.41801.
ieee: K. Valosková et al., “A conserved major facilitator superfamily member
orchestrates a subset of O-glycosylation to aid macrophage tissue invasion,” eLife,
vol. 8. eLife Sciences Publications, 2019.
ista: Valosková K, Bicher J, Roblek M, Emtenani S, György A, Misova M, Ratheesh
A, Rodrigues P, Shkarina K, Larsen ISB, Vakhrushev SY, Clausen H, Siekhaus DE.
2019. A conserved major facilitator superfamily member orchestrates a subset of
O-glycosylation to aid macrophage tissue invasion. eLife. 8, e41801.
mla: Valosková, Katarina, et al. “A Conserved Major Facilitator Superfamily Member
Orchestrates a Subset of O-Glycosylation to Aid Macrophage Tissue Invasion.” ELife,
vol. 8, e41801, eLife Sciences Publications, 2019, doi:10.7554/elife.41801.
short: K. Valosková, J. Bicher, M. Roblek, S. Emtenani, A. György, M. Misova, A.
Ratheesh, P. Rodrigues, K. Shkarina, I.S.B. Larsen, S.Y. Vakhrushev, H. Clausen,
D.E. Siekhaus, ELife 8 (2019).
date_created: 2019-03-28T13:37:45Z
date_published: 2019-03-26T00:00:00Z
date_updated: 2024-03-25T23:30:15Z
day: '26'
ddc:
- '570'
department:
- _id: DaSi
doi: 10.7554/elife.41801
ec_funded: 1
external_id:
isi:
- '000462530200001'
file:
- access_level: open_access
checksum: cc0d1a512559d52e7e7cb0e9b9854b40
content_type: application/pdf
creator: dernst
date_created: 2019-03-28T14:00:41Z
date_updated: 2020-07-14T12:47:23Z
file_id: '6188'
file_name: 2019_eLife_Valoskova.pdf
file_size: 4496017
relation: main_file
file_date_updated: 2020-07-14T12:47:23Z
has_accepted_license: '1'
intvolume: ' 8'
isi: 1
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
project:
- _id: 253CDE40-B435-11E9-9278-68D0E5697425
grant_number: '24283'
name: Examination of the role of a MFS transporter in the migration of Drosophila
immune cells
- _id: 253B6E48-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P29638
name: The role of Drosophila TNF alpha in immune cell invasion
- _id: 2536F660-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '334077'
name: Investigating the role of transporters in invasive migration through junctions
- _id: 25388084-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '329540'
name: 'Breaking barriers: Investigating the junctional and mechanobiological changes
underlying the ability of Drosophila immune cells to invade an epithelium'
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '665385'
name: International IST Doctoral Program
publication: eLife
publication_identifier:
issn:
- 2050-084X
publication_status: published
publisher: eLife Sciences Publications
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/new-gene-potentially-involved-in-metastasis-identified/
record:
- id: '6530'
relation: dissertation_contains
- id: '8983'
relation: dissertation_contains
status: public
- id: '6546'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: A conserved major facilitator superfamily member orchestrates a subset of O-glycosylation
to aid macrophage tissue invasion
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 8
year: '2019'
...
---
_id: '308'
abstract:
- lang: eng
text: Migrating cells penetrate tissue barriers during development, inflammatory
responses, and tumor metastasis. We study if migration in vivo in such three-dimensionally
confined environments requires changes in the mechanical properties of the surrounding
cells using embryonic Drosophila melanogaster hemocytes, also called macrophages,
as a model. We find that macrophage invasion into the germband through transient
separation of the apposing ectoderm and mesoderm requires cell deformations and
reductions in apical tension in the ectoderm. Interestingly, the genetic pathway
governing these mechanical shifts acts downstream of the only known tumor necrosis
factor superfamily member in Drosophila, Eiger, and its receptor, Grindelwald.
Eiger-Grindelwald signaling reduces levels of active Myosin in the germband ectodermal
cortex through the localization of a Crumbs complex component, Patj (Pals-1-associated
tight junction protein). We therefore elucidate a distinct molecular pathway that
controls tissue tension and demonstrate the importance of such regulation for
invasive migration in vivo.
acknowledged_ssus:
- _id: SSU
article_processing_charge: No
article_type: original
author:
- first_name: Aparna
full_name: Ratheesh, Aparna
id: 2F064CFE-F248-11E8-B48F-1D18A9856A87
last_name: Ratheesh
orcid: 0000-0001-7190-0776
- first_name: Julia
full_name: Biebl, Julia
id: 3CCBB46E-F248-11E8-B48F-1D18A9856A87
last_name: Biebl
- first_name: Michael
full_name: Smutny, Michael
last_name: Smutny
- first_name: Jana
full_name: Veselá, Jana
id: 433253EE-F248-11E8-B48F-1D18A9856A87
last_name: Veselá
- first_name: Ekaterina
full_name: Papusheva, Ekaterina
id: 41DB591E-F248-11E8-B48F-1D18A9856A87
last_name: Papusheva
- first_name: Gabriel
full_name: Krens, Gabriel
id: 2B819732-F248-11E8-B48F-1D18A9856A87
last_name: Krens
orcid: 0000-0003-4761-5996
- first_name: Walter
full_name: Kaufmann, Walter
id: 3F99E422-F248-11E8-B48F-1D18A9856A87
last_name: Kaufmann
orcid: 0000-0001-9735-5315
- first_name: Attila
full_name: György, Attila
id: 3BCEDBE0-F248-11E8-B48F-1D18A9856A87
last_name: György
orcid: 0000-0002-1819-198X
- first_name: Alessandra M
full_name: Casano, Alessandra M
id: 3DBA3F4E-F248-11E8-B48F-1D18A9856A87
last_name: Casano
orcid: 0000-0002-6009-6804
- first_name: Daria E
full_name: Siekhaus, Daria E
id: 3D224B9E-F248-11E8-B48F-1D18A9856A87
last_name: Siekhaus
orcid: 0000-0001-8323-8353
citation:
ama: Ratheesh A, Bicher J, Smutny M, et al. Drosophila TNF modulates tissue tension
in the embryo to facilitate macrophage invasive migration. Developmental Cell.
2018;45(3):331-346. doi:10.1016/j.devcel.2018.04.002
apa: Ratheesh, A., Bicher, J., Smutny, M., Veselá, J., Papusheva, E., Krens, G.,
… Siekhaus, D. E. (2018). Drosophila TNF modulates tissue tension in the embryo
to facilitate macrophage invasive migration. Developmental Cell. Elsevier.
https://doi.org/10.1016/j.devcel.2018.04.002
chicago: Ratheesh, Aparna, Julia Bicher, Michael Smutny, Jana Veselá, Ekaterina
Papusheva, Gabriel Krens, Walter Kaufmann, Attila György, Alessandra M Casano,
and Daria E Siekhaus. “Drosophila TNF Modulates Tissue Tension in the Embryo to
Facilitate Macrophage Invasive Migration.” Developmental Cell. Elsevier,
2018. https://doi.org/10.1016/j.devcel.2018.04.002.
ieee: A. Ratheesh et al., “Drosophila TNF modulates tissue tension in the
embryo to facilitate macrophage invasive migration,” Developmental Cell,
vol. 45, no. 3. Elsevier, pp. 331–346, 2018.
ista: Ratheesh A, Bicher J, Smutny M, Veselá J, Papusheva E, Krens G, Kaufmann W,
György A, Casano AM, Siekhaus DE. 2018. Drosophila TNF modulates tissue tension
in the embryo to facilitate macrophage invasive migration. Developmental Cell.
45(3), 331–346.
mla: Ratheesh, Aparna, et al. “Drosophila TNF Modulates Tissue Tension in the Embryo
to Facilitate Macrophage Invasive Migration.” Developmental Cell, vol.
45, no. 3, Elsevier, 2018, pp. 331–46, doi:10.1016/j.devcel.2018.04.002.
short: A. Ratheesh, J. Bicher, M. Smutny, J. Veselá, E. Papusheva, G. Krens, W.
Kaufmann, A. György, A.M. Casano, D.E. Siekhaus, Developmental Cell 45 (2018)
331–346.
date_created: 2018-12-11T11:45:44Z
date_published: 2018-05-07T00:00:00Z
date_updated: 2023-09-11T13:22:13Z
day: '07'
department:
- _id: DaSi
- _id: CaHe
- _id: Bio
- _id: EM-Fac
- _id: MiSi
doi: 10.1016/j.devcel.2018.04.002
ec_funded: 1
external_id:
isi:
- '000432461400009'
pmid:
- '29738712'
intvolume: ' 45'
isi: 1
issue: '3'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1016/j.devcel.2018.04.002
month: '05'
oa: 1
oa_version: Published Version
page: 331 - 346
pmid: 1
project:
- _id: 253B6E48-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P29638
name: Drosophila TNFa´s Funktion in Immunzellen
- _id: 2536F660-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '334077'
name: Investigating the role of transporters in invasive migration through junctions
publication: Developmental Cell
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/cells-change-tension-to-make-tissue-barriers-easier-to-get-through/
scopus_import: '1'
status: public
title: Drosophila TNF modulates tissue tension in the embryo to facilitate macrophage
invasive migration
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 45
year: '2018'
...
---
_id: '544'
abstract:
- lang: eng
text: Drosophila melanogaster plasmatocytes, the phagocytic cells among hemocytes,
are essential for immune responses, but also play key roles from early development
to death through their interactions with other cell types. They regulate homeostasis
and signaling during development, stem cell proliferation, metabolism, cancer,
wound responses and aging, displaying intriguing molecular and functional conservation
with vertebrate macrophages. Given the relative ease of genetics in Drosophila
compared to vertebrates, tools permitting visualization and genetic manipulation
of plasmatocytes and surrounding tissues independently at all stages would greatly
aid in fully understanding these processes, but are lacking. Here we describe
a comprehensive set of transgenic lines that allow this. These include extremely
brightly fluorescing mCherry-based lines that allow GAL4-independent visualization
of plasmatocyte nuclei, cytoplasm or actin cytoskeleton from embryonic Stage 8
through adulthood in both live and fixed samples even as heterozygotes, greatly
facilitating screening. These lines allow live visualization and tracking of embryonic
plasmatocytes, as well as larval plasmatocytes residing at the body wall or flowing
with the surrounding hemolymph. With confocal imaging, interactions of plasmatocytes
and inner tissues can be seen in live or fixed embryos, larvae and adults. They
permit efficient GAL4-independent FACS analysis/sorting of plasmatocytes throughout
life. To facilitate genetic analysis of reciprocal signaling, we have also made
a plasmatocyte-expressing QF2 line that in combination with extant GAL4 drivers
allows independent genetic manipulation of both plasmatocytes and surrounding
tissues, and a GAL80 line that blocks GAL4 drivers from affecting plasmatocytes,
both of which function from the early embryo to the adult.
acknowledged_ssus:
- _id: LifeSc
acknowledgement: ' A. Ratheesh also by Marie Curie IIF GA-2012-32950BB:DICJI, Marko
Roblek by the provincial government of Lower Austria, K. Valoskova and S. Wachner
by DOC Fellowships from the Austrian Academy of Sciences, '
article_processing_charge: No
author:
- first_name: Attila
full_name: György, Attila
id: 3BCEDBE0-F248-11E8-B48F-1D18A9856A87
last_name: György
orcid: 0000-0002-1819-198X
- first_name: Marko
full_name: Roblek, Marko
id: 3047D808-F248-11E8-B48F-1D18A9856A87
last_name: Roblek
orcid: 0000-0001-9588-1389
- first_name: Aparna
full_name: Ratheesh, Aparna
id: 2F064CFE-F248-11E8-B48F-1D18A9856A87
last_name: Ratheesh
orcid: 0000-0001-7190-0776
- first_name: Katarina
full_name: Valosková, Katarina
id: 46F146FC-F248-11E8-B48F-1D18A9856A87
last_name: Valosková
- first_name: Vera
full_name: Belyaeva, Vera
id: 47F080FE-F248-11E8-B48F-1D18A9856A87
last_name: Belyaeva
- first_name: Stephanie
full_name: Wachner, Stephanie
id: 2A95E7B0-F248-11E8-B48F-1D18A9856A87
last_name: Wachner
- first_name: Yutaka
full_name: Matsubayashi, Yutaka
last_name: Matsubayashi
- first_name: Besaiz
full_name: Sanchez Sanchez, Besaiz
last_name: Sanchez Sanchez
- first_name: Brian
full_name: Stramer, Brian
last_name: Stramer
- first_name: Daria E
full_name: Siekhaus, Daria E
id: 3D224B9E-F248-11E8-B48F-1D18A9856A87
last_name: Siekhaus
orcid: 0000-0001-8323-8353
citation:
ama: 'György A, Roblek M, Ratheesh A, et al. Tools allowing independent visualization
and genetic manipulation of Drosophila melanogaster macrophages and surrounding
tissues. G3: Genes, Genomes, Genetics. 2018;8(3):845-857. doi:10.1534/g3.117.300452'
apa: 'György, A., Roblek, M., Ratheesh, A., Valosková, K., Belyaeva, V., Wachner,
S., … Siekhaus, D. E. (2018). Tools allowing independent visualization and genetic
manipulation of Drosophila melanogaster macrophages and surrounding tissues. G3:
Genes, Genomes, Genetics. Genetics Society of America. https://doi.org/10.1534/g3.117.300452'
chicago: 'György, Attila, Marko Roblek, Aparna Ratheesh, Katarina Valosková, Vera
Belyaeva, Stephanie Wachner, Yutaka Matsubayashi, Besaiz Sanchez Sanchez, Brian
Stramer, and Daria E Siekhaus. “Tools Allowing Independent Visualization and Genetic
Manipulation of Drosophila Melanogaster Macrophages and Surrounding Tissues.”
G3: Genes, Genomes, Genetics. Genetics Society of America, 2018. https://doi.org/10.1534/g3.117.300452.'
ieee: 'A. György et al., “Tools allowing independent visualization and genetic
manipulation of Drosophila melanogaster macrophages and surrounding tissues,”
G3: Genes, Genomes, Genetics, vol. 8, no. 3. Genetics Society of America,
pp. 845–857, 2018.'
ista: 'György A, Roblek M, Ratheesh A, Valosková K, Belyaeva V, Wachner S, Matsubayashi
Y, Sanchez Sanchez B, Stramer B, Siekhaus DE. 2018. Tools allowing independent
visualization and genetic manipulation of Drosophila melanogaster macrophages
and surrounding tissues. G3: Genes, Genomes, Genetics. 8(3), 845–857.'
mla: 'György, Attila, et al. “Tools Allowing Independent Visualization and Genetic
Manipulation of Drosophila Melanogaster Macrophages and Surrounding Tissues.”
G3: Genes, Genomes, Genetics, vol. 8, no. 3, Genetics Society of America,
2018, pp. 845–57, doi:10.1534/g3.117.300452.'
short: 'A. György, M. Roblek, A. Ratheesh, K. Valosková, V. Belyaeva, S. Wachner,
Y. Matsubayashi, B. Sanchez Sanchez, B. Stramer, D.E. Siekhaus, G3: Genes, Genomes,
Genetics 8 (2018) 845–857.'
date_created: 2018-12-11T11:47:05Z
date_published: 2018-03-01T00:00:00Z
date_updated: 2024-03-25T23:30:15Z
day: '01'
ddc:
- '570'
department:
- _id: DaSi
doi: 10.1534/g3.117.300452
ec_funded: 1
external_id:
isi:
- '000426693300011'
file:
- access_level: open_access
checksum: 7d9d28b915159078a4ca7add568010e8
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:11:48Z
date_updated: 2020-07-14T12:46:56Z
file_id: '4905'
file_name: IST-2018-990-v1+1_2018_Gyoergy_Tools_allowing.pdf
file_size: 2251222
relation: main_file
file_date_updated: 2020-07-14T12:46:56Z
has_accepted_license: '1'
intvolume: ' 8'
isi: 1
issue: '3'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
page: 845 - 857
project:
- _id: 253B6E48-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P29638
name: Drosophila TNFa´s Funktion in Immunzellen
- _id: 253B6E48-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: P29638
name: The role of Drosophila TNF alpha in immune cell invasion
- _id: 2637E9C0-B435-11E9-9278-68D0E5697425
grant_number: 'LSC16-021 '
name: Investigating the role of the novel major superfamily facilitator transporter
family member MFSD1 in metastasis
- _id: 2536F660-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '334077'
name: Investigating the role of transporters in invasive migration through junctions
publication: 'G3: Genes, Genomes, Genetics'
publication_status: published
publisher: Genetics Society of America
publist_id: '7271'
pubrep_id: '990'
quality_controlled: '1'
related_material:
record:
- id: '6530'
relation: research_paper
- id: '6543'
relation: research_paper
- id: '11193'
relation: dissertation_contains
status: public
- id: '6546'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Tools allowing independent visualization and genetic manipulation of Drosophila
melanogaster macrophages and surrounding tissues
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 8
year: '2018'
...
---
_id: '1475'
abstract:
- lang: eng
text: The actin cytoskeleton plays important roles in the formation and internalization
of endocytic vesicles. In yeast, endocytic vesicles move towards early endosomes
along actin cables, however, the molecular machinery regulating interaction between
endocytic vesicles and actin cables is poorly understood. The Eps15-like protein
Pan1p plays a key role in actin-mediated endocytosis and is negatively regulated
by Ark1 and Prk1 kinases. Here we show that pan1 mutated to prevent phosphorylation
at all 18 threonines, pan1-18TA, displayed almost the same endocytic defect as
ark1Δ prk1Δ cells, and contained abnormal actin concentrations including several
endocytic compartments. Early endosomes were highly localized in the actin concentrations
and displayed movement along actin cables. The dephosphorylated form of Pan1p
also caused stable associations between endocytic vesicles and actin cables, and
between endocytic vesicles and endosomes. Thus Pan1 phosphorylation is part of
a novel mechanism that regulates endocytic compartment interactions with each
other and with actin cables.
article_number: e10276
author:
- first_name: Junko
full_name: Toshima, Junko
last_name: Toshima
- first_name: Eri
full_name: Furuya, Eri
last_name: Furuya
- first_name: Makoto
full_name: Nagano, Makoto
last_name: Nagano
- first_name: Chisa
full_name: Kanno, Chisa
last_name: Kanno
- first_name: Yuta
full_name: Sakamoto, Yuta
last_name: Sakamoto
- first_name: Masashi
full_name: Ebihara, Masashi
last_name: Ebihara
- first_name: Daria E
full_name: Siekhaus, Daria E
id: 3D224B9E-F248-11E8-B48F-1D18A9856A87
last_name: Siekhaus
orcid: 0000-0001-8323-8353
- first_name: Jiro
full_name: Toshima, Jiro
last_name: Toshima
citation:
ama: Toshima J, Furuya E, Nagano M, et al. Yeast Eps15-like endocytic protein Pan1p
regulates the interaction between endocytic vesicles, endosomes and the actin
cytoskeleton. eLife. 2016;5(February 2016). doi:10.7554/eLife.10276
apa: Toshima, J., Furuya, E., Nagano, M., Kanno, C., Sakamoto, Y., Ebihara, M.,
… Toshima, J. (2016). Yeast Eps15-like endocytic protein Pan1p regulates the interaction
between endocytic vesicles, endosomes and the actin cytoskeleton. ELife.
eLife Sciences Publications. https://doi.org/10.7554/eLife.10276
chicago: Toshima, Junko, Eri Furuya, Makoto Nagano, Chisa Kanno, Yuta Sakamoto,
Masashi Ebihara, Daria E Siekhaus, and Jiro Toshima. “Yeast Eps15-like Endocytic
Protein Pan1p Regulates the Interaction between Endocytic Vesicles, Endosomes
and the Actin Cytoskeleton.” ELife. eLife Sciences Publications, 2016.
https://doi.org/10.7554/eLife.10276.
ieee: J. Toshima et al., “Yeast Eps15-like endocytic protein Pan1p regulates
the interaction between endocytic vesicles, endosomes and the actin cytoskeleton,”
eLife, vol. 5, no. February 2016. eLife Sciences Publications, 2016.
ista: Toshima J, Furuya E, Nagano M, Kanno C, Sakamoto Y, Ebihara M, Siekhaus DE,
Toshima J. 2016. Yeast Eps15-like endocytic protein Pan1p regulates the interaction
between endocytic vesicles, endosomes and the actin cytoskeleton. eLife. 5(February
2016), e10276.
mla: Toshima, Junko, et al. “Yeast Eps15-like Endocytic Protein Pan1p Regulates
the Interaction between Endocytic Vesicles, Endosomes and the Actin Cytoskeleton.”
ELife, vol. 5, no. February 2016, e10276, eLife Sciences Publications,
2016, doi:10.7554/eLife.10276.
short: J. Toshima, E. Furuya, M. Nagano, C. Kanno, Y. Sakamoto, M. Ebihara, D.E.
Siekhaus, J. Toshima, ELife 5 (2016).
date_created: 2018-12-11T11:52:14Z
date_published: 2016-02-25T00:00:00Z
date_updated: 2021-01-12T06:50:59Z
day: '25'
ddc:
- '570'
department:
- _id: DaSi
doi: 10.7554/eLife.10276
ec_funded: 1
file:
- access_level: open_access
checksum: d1cc44870580756ba8badd8e41adfdb5
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:10:08Z
date_updated: 2020-07-14T12:44:56Z
file_id: '4793'
file_name: IST-2016-529-v1+1_elife-10276-v1.pdf
file_size: 5198001
relation: main_file
file_date_updated: 2020-07-14T12:44:56Z
has_accepted_license: '1'
intvolume: ' 5'
issue: February 2016
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
project:
- _id: 2536F660-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '334077'
name: Investigating the role of transporters in invasive migration through junctions
publication: eLife
publication_status: published
publisher: eLife Sciences Publications
publist_id: '5721'
pubrep_id: '529'
quality_controlled: '1'
scopus_import: 1
status: public
title: Yeast Eps15-like endocytic protein Pan1p regulates the interaction between
endocytic vesicles, endosomes and the actin cytoskeleton
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 5
year: '2016'
...
---
_id: '1476'
abstract:
- lang: eng
text: The dynamic assembly and disassembly of actin filaments is essential for the
formation and transport of vesicles during endocytosis. In yeast, two types of
actin structures, namely cortical patches and cytoplasmic cables, play a direct
role in endocytosis, but how their interaction is regulated remains unclear. Here,
we show that Srv2/CAP, an evolutionarily conserved actin regulator, is required
for efficient endocytosis owing to its role in the formation of the actin patches
that aid initial vesicle invagination and of the actin cables that these move
along. Deletion of the SRV2 gene resulted in the appearance of aberrant fragmented
actin cables that frequently moved past actin patches, the sites of endocytosis.
We find that the C-terminal CARP domain of Srv2p is vitally important for the
proper assembly of actin patches and cables; we also demonstrate that the N-terminal
helical folded domain of Srv2 is required for its localization to actin patches,
specifically to the ADP-actin rich region through an interaction with cofilin.
These results demonstrate the in vivo roles of Srv2p in the regulation of the
actin cytoskeleton during clathrin-mediated endocytosis
acknowledgement: We are grateful to Anthony Bretscher (Cornell University, NY) for
providing the bni1-12 bnr1Δ (Y4135) strain. J.Y.T. was supported by a Japan Society
for the Promotion of Science (JSPS) KAKENHI grant [grant number 26440067]; the Takeda
Science Foundation; and the Novartis Foundation (Japan). J.T. was supported by a
JSPS KAKENHI grant [grant number 25440054]; the Takeda Science Foundation; and the
Kurata Memorial Hitachi Science and Technology Foundation. D.E.S. was supported
by the European Union [grant number PCIG12-GA-2012-334077].
author:
- first_name: Junko
full_name: Toshima, Junko
last_name: Toshima
- first_name: Chika
full_name: Horikomi, Chika
last_name: Horikomi
- first_name: Asuka
full_name: Okada, Asuka
last_name: Okada
- first_name: Makiko
full_name: Hatori, Makiko
last_name: Hatori
- first_name: Makoto
full_name: Nagano, Makoto
last_name: Nagano
- first_name: Atsushi
full_name: Masuda, Atsushi
last_name: Masuda
- first_name: Wataru
full_name: Yamamoto, Wataru
last_name: Yamamoto
- first_name: Daria E
full_name: Siekhaus, Daria E
id: 3D224B9E-F248-11E8-B48F-1D18A9856A87
last_name: Siekhaus
orcid: 0000-0001-8323-8353
- first_name: Jiro
full_name: Toshima, Jiro
last_name: Toshima
citation:
ama: Toshima J, Horikomi C, Okada A, et al. Srv2/CAP is required for polarized actin
cable assembly and patch internalization during clathrin-mediated endocytosis.
Journal of Cell Science. 2016;129(2):367-379. doi:10.1242/jcs.176651
apa: Toshima, J., Horikomi, C., Okada, A., Hatori, M., Nagano, M., Masuda, A., …
Toshima, J. (2016). Srv2/CAP is required for polarized actin cable assembly and
patch internalization during clathrin-mediated endocytosis. Journal of Cell
Science. Company of Biologists. https://doi.org/10.1242/jcs.176651
chicago: Toshima, Junko, Chika Horikomi, Asuka Okada, Makiko Hatori, Makoto Nagano,
Atsushi Masuda, Wataru Yamamoto, Daria E Siekhaus, and Jiro Toshima. “Srv2/CAP
Is Required for Polarized Actin Cable Assembly and Patch Internalization during
Clathrin-Mediated Endocytosis.” Journal of Cell Science. Company of Biologists,
2016. https://doi.org/10.1242/jcs.176651.
ieee: J. Toshima et al., “Srv2/CAP is required for polarized actin cable
assembly and patch internalization during clathrin-mediated endocytosis,” Journal
of Cell Science, vol. 129, no. 2. Company of Biologists, pp. 367–379, 2016.
ista: Toshima J, Horikomi C, Okada A, Hatori M, Nagano M, Masuda A, Yamamoto W,
Siekhaus DE, Toshima J. 2016. Srv2/CAP is required for polarized actin cable assembly
and patch internalization during clathrin-mediated endocytosis. Journal of Cell
Science. 129(2), 367–379.
mla: Toshima, Junko, et al. “Srv2/CAP Is Required for Polarized Actin Cable Assembly
and Patch Internalization during Clathrin-Mediated Endocytosis.” Journal of
Cell Science, vol. 129, no. 2, Company of Biologists, 2016, pp. 367–79, doi:10.1242/jcs.176651.
short: J. Toshima, C. Horikomi, A. Okada, M. Hatori, M. Nagano, A. Masuda, W. Yamamoto,
D.E. Siekhaus, J. Toshima, Journal of Cell Science 129 (2016) 367–379.
date_created: 2018-12-11T11:52:14Z
date_published: 2016-01-15T00:00:00Z
date_updated: 2021-01-12T06:51:00Z
day: '15'
ddc:
- '570'
- '576'
department:
- _id: DaSi
doi: 10.1242/jcs.176651
ec_funded: 1
file:
- access_level: open_access
checksum: 2da0a09149a9ed956cdf79a95c17f08a
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:11:08Z
date_updated: 2020-07-14T12:44:56Z
file_id: '4861'
file_name: IST-2017-767-v1+1_367.full.pdf
file_size: 7176912
relation: main_file
file_date_updated: 2020-07-14T12:44:56Z
has_accepted_license: '1'
intvolume: ' 129'
issue: '2'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
page: 367 - 379
project:
- _id: 2536F660-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '334077'
name: Investigating the role of transporters in invasive migration through junctions
publication: Journal of Cell Science
publication_status: published
publisher: Company of Biologists
publist_id: '5720'
pubrep_id: '767'
quality_controlled: '1'
scopus_import: 1
status: public
title: Srv2/CAP is required for polarized actin cable assembly and patch internalization
during clathrin-mediated endocytosis
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 129
year: '2016'
...
---
_id: '1712'
abstract:
- lang: eng
text: The majority of immune cells in Drosophila melanogaster are plasmatocytes;
they carry out similar functions to vertebrate macrophages, influencing development
as well as protecting against infection and cancer. Plasmatocytes, sometimes referred
to with the broader term of hemocytes, migrate widely during embryonic development
and cycle in the larvae between sessile and circulating positions. Here we discuss
the similarities of plasmatocyte developmental migration and its functions to
that of vertebrate macrophages, considering the recent controversy regarding the
functions of Drosophila PDGF/VEGF related ligands. We also examine recent findings
on the significance of adhesion for plasmatocyte migration in the embryo, as well
as proliferation, trans-differentiation, and tumor responses in the larva. We
spotlight parallels throughout to vertebrate immune responses.
author:
- first_name: Aparna
full_name: Ratheesh, Aparna
id: 2F064CFE-F248-11E8-B48F-1D18A9856A87
last_name: Ratheesh
- first_name: Vera
full_name: Belyaeva, Vera
id: 47F080FE-F248-11E8-B48F-1D18A9856A87
last_name: Belyaeva
- first_name: Daria E
full_name: Siekhaus, Daria E
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last_name: Siekhaus
orcid: 0000-0001-8323-8353
citation:
ama: Ratheesh A, Belyaeva V, Siekhaus DE. Drosophila immune cell migration and adhesion
during embryonic development and larval immune responses. Current Opinion in
Cell Biology. 2015;36(10):71-79. doi:10.1016/j.ceb.2015.07.003
apa: Ratheesh, A., Belyaeva, V., & Siekhaus, D. E. (2015). Drosophila immune
cell migration and adhesion during embryonic development and larval immune responses.
Current Opinion in Cell Biology. Elsevier. https://doi.org/10.1016/j.ceb.2015.07.003
chicago: Ratheesh, Aparna, Vera Belyaeva, and Daria E Siekhaus. “Drosophila Immune
Cell Migration and Adhesion during Embryonic Development and Larval Immune Responses.”
Current Opinion in Cell Biology. Elsevier, 2015. https://doi.org/10.1016/j.ceb.2015.07.003.
ieee: A. Ratheesh, V. Belyaeva, and D. E. Siekhaus, “Drosophila immune cell migration
and adhesion during embryonic development and larval immune responses,” Current
Opinion in Cell Biology, vol. 36, no. 10. Elsevier, pp. 71–79, 2015.
ista: Ratheesh A, Belyaeva V, Siekhaus DE. 2015. Drosophila immune cell migration
and adhesion during embryonic development and larval immune responses. Current
Opinion in Cell Biology. 36(10), 71–79.
mla: Ratheesh, Aparna, et al. “Drosophila Immune Cell Migration and Adhesion during
Embryonic Development and Larval Immune Responses.” Current Opinion in Cell
Biology, vol. 36, no. 10, Elsevier, 2015, pp. 71–79, doi:10.1016/j.ceb.2015.07.003.
short: A. Ratheesh, V. Belyaeva, D.E. Siekhaus, Current Opinion in Cell Biology
36 (2015) 71–79.
date_created: 2018-12-11T11:53:36Z
date_published: 2015-10-01T00:00:00Z
date_updated: 2021-01-12T06:52:41Z
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name: Investigating the role of transporters in invasive migration through junctions
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title: Drosophila immune cell migration and adhesion during embryonic development
and larval immune responses
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