---
_id: '661'
abstract:
- lang: eng
  text: During embryonic development, mechanical forces are essential for cellular
    rearrangements driving tissue morphogenesis. Here, we show that in the early zebrafish
    embryo, friction forces are generated at the interface between anterior axial
    mesoderm (prechordal plate, ppl) progenitors migrating towards the animal pole
    and neurectoderm progenitors moving in the opposite direction towards the vegetal
    pole of the embryo. These friction forces lead to global rearrangement of cells
    within the neurectoderm and determine the position of the neural anlage. Using
    a combination of experiments and simulations, we show that this process depends
    on hydrodynamic coupling between neurectoderm and ppl as a result of E-cadherin-mediated
    adhesion between those tissues. Our data thus establish the emergence of friction
    forces at the interface between moving tissues as a critical force-generating
    process shaping the embryo.
acknowledged_ssus:
- _id: SSU
author:
- first_name: Michael
  full_name: Smutny, Michael
  id: 3FE6E4E8-F248-11E8-B48F-1D18A9856A87
  last_name: Smutny
  orcid: 0000-0002-5920-9090
- first_name: Zsuzsa
  full_name: Ákos, Zsuzsa
  last_name: Ákos
- first_name: Silvia
  full_name: Grigolon, Silvia
  last_name: Grigolon
- first_name: Shayan
  full_name: Shamipour, Shayan
  id: 40B34FE2-F248-11E8-B48F-1D18A9856A87
  last_name: Shamipour
- first_name: Verena
  full_name: Ruprecht, Verena
  last_name: Ruprecht
- first_name: Daniel
  full_name: Capek, Daniel
  id: 31C42484-F248-11E8-B48F-1D18A9856A87
  last_name: Capek
  orcid: 0000-0001-5199-9940
- first_name: Martin
  full_name: Behrndt, Martin
  id: 3ECECA3A-F248-11E8-B48F-1D18A9856A87
  last_name: Behrndt
- first_name: Ekaterina
  full_name: Papusheva, Ekaterina
  id: 41DB591E-F248-11E8-B48F-1D18A9856A87
  last_name: Papusheva
- first_name: Masazumi
  full_name: Tada, Masazumi
  last_name: Tada
- first_name: Björn
  full_name: Hof, Björn
  id: 3A374330-F248-11E8-B48F-1D18A9856A87
  last_name: Hof
  orcid: 0000-0003-2057-2754
- first_name: Tamás
  full_name: Vicsek, Tamás
  last_name: Vicsek
- first_name: Guillaume
  full_name: Salbreux, Guillaume
  last_name: Salbreux
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
citation:
  ama: Smutny M, Ákos Z, Grigolon S, et al. Friction forces position the neural anlage.
    <i>Nature Cell Biology</i>. 2017;19:306-317. doi:<a href="https://doi.org/10.1038/ncb3492">10.1038/ncb3492</a>
  apa: Smutny, M., Ákos, Z., Grigolon, S., Shamipour, S., Ruprecht, V., Capek, D.,
    … Heisenberg, C.-P. J. (2017). Friction forces position the neural anlage. <i>Nature
    Cell Biology</i>. Nature Publishing Group. <a href="https://doi.org/10.1038/ncb3492">https://doi.org/10.1038/ncb3492</a>
  chicago: Smutny, Michael, Zsuzsa Ákos, Silvia Grigolon, Shayan Shamipour, Verena
    Ruprecht, Daniel Capek, Martin Behrndt, et al. “Friction Forces Position the Neural
    Anlage.” <i>Nature Cell Biology</i>. Nature Publishing Group, 2017. <a href="https://doi.org/10.1038/ncb3492">https://doi.org/10.1038/ncb3492</a>.
  ieee: M. Smutny <i>et al.</i>, “Friction forces position the neural anlage,” <i>Nature
    Cell Biology</i>, vol. 19. Nature Publishing Group, pp. 306–317, 2017.
  ista: Smutny M, Ákos Z, Grigolon S, Shamipour S, Ruprecht V, Capek D, Behrndt M,
    Papusheva E, Tada M, Hof B, Vicsek T, Salbreux G, Heisenberg C-PJ. 2017. Friction
    forces position the neural anlage. Nature Cell Biology. 19, 306–317.
  mla: Smutny, Michael, et al. “Friction Forces Position the Neural Anlage.” <i>Nature
    Cell Biology</i>, vol. 19, Nature Publishing Group, 2017, pp. 306–17, doi:<a href="https://doi.org/10.1038/ncb3492">10.1038/ncb3492</a>.
  short: M. Smutny, Z. Ákos, S. Grigolon, S. Shamipour, V. Ruprecht, D. Capek, M.
    Behrndt, E. Papusheva, M. Tada, B. Hof, T. Vicsek, G. Salbreux, C.-P.J. Heisenberg,
    Nature Cell Biology 19 (2017) 306–317.
date_created: 2018-12-11T11:47:46Z
date_published: 2017-03-27T00:00:00Z
date_updated: 2024-03-25T23:30:21Z
day: '27'
department:
- _id: CaHe
- _id: BjHo
- _id: Bio
doi: 10.1038/ncb3492
ec_funded: 1
external_id:
  pmid:
  - '28346437'
intvolume: '        19'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://europepmc.org/articles/pmc5635970
month: '03'
oa: 1
oa_version: Submitted Version
page: 306 - 317
pmid: 1
project:
- _id: 25152F3A-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '306589'
  name: Decoding the complexity of turbulence at its origin
- _id: 252ABD0A-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: I 930-B20
  name: Control of Epithelial Cell Layer Spreading in Zebrafish
publication: Nature Cell Biology
publication_identifier:
  issn:
  - '14657392'
publication_status: published
publisher: Nature Publishing Group
publist_id: '7074'
quality_controlled: '1'
related_material:
  record:
  - id: '50'
    relation: dissertation_contains
    status: public
  - id: '8350'
    relation: dissertation_contains
    status: public
scopus_import: 1
status: public
title: Friction forces position the neural anlage
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 19
year: '2017'
...
---
_id: '1249'
abstract:
- lang: eng
  text: 'Actin and myosin assemble into a thin layer of a highly dynamic network underneath
    the membrane of eukaryotic cells. This network generates the forces that drive
    cell- and tissue-scale morphogenetic processes. The effective material properties
    of this active network determine large-scale deformations and other morphogenetic
    events. For example, the characteristic time of stress relaxation (the Maxwell
    time τM) in the actomyosin sets the timescale of large-scale deformation of the
    cortex. Similarly, the characteristic length of stress propagation (the hydrodynamic
    length λ) sets the length scale of slow deformations, and a large hydrodynamic
    length is a prerequisite for long-ranged cortical flows. Here we introduce a method
    to determine physical parameters of the actomyosin cortical layer in vivo directly
    from laser ablation experiments. For this we investigate the cortical response
    to laser ablation in the one-cell-stage Caenorhabditis elegans embryo and in the
    gastrulating zebrafish embryo. These responses can be interpreted using a coarse-grained
    physical description of the cortex in terms of a two-dimensional thin film of
    an active viscoelastic gel. To determine the Maxwell time τM, the hydrodynamic
    length λ, the ratio of active stress ζΔμ, and per-area friction γ, we evaluated
    the response to laser ablation in two different ways: by quantifying flow and
    density fields as a function of space and time, and by determining the time evolution
    of the shape of the ablated region. Importantly, both methods provide best-fit
    physical parameters that are in close agreement with each other and that are similar
    to previous estimates in the two systems. Our method provides an accurate and
    robust means for measuring physical parameters of the actomyosin cortical layer.
    It can be useful for investigations of actomyosin mechanics at the cellular-scale,
    but also for providing insights into the active mechanics processes that govern
    tissue-scale morphogenesis.'
acknowledgement: S.W.G. acknowledges support by grant no. 281903 from the European
  Research Council and by grant No. GR-7271/2-1 from the Deutsche Forschungsgemeinschaft.
  S.W.G. and C.-P.H. acknowledge support through a grant from the Fonds zur Förderung
  der Wissenschaftlichen Forschung and the Deutsche Forschungsgemeinschaft (No. I930-B20).
  We are grateful to Daniel Dickinson for providing the LP133 C. elegans strain. We
  thank G. Salbreux, V. K. Krishnamurthy, and J. S. Bois for fruitful discussions.
author:
- first_name: Arnab
  full_name: Saha, Arnab
  last_name: Saha
- first_name: Masatoshi
  full_name: Nishikawa, Masatoshi
  last_name: Nishikawa
- first_name: Martin
  full_name: Behrndt, Martin
  id: 3ECECA3A-F248-11E8-B48F-1D18A9856A87
  last_name: Behrndt
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
- first_name: Frank
  full_name: Julicher, Frank
  last_name: Julicher
- first_name: Stephan
  full_name: Grill, Stephan
  last_name: Grill
citation:
  ama: Saha A, Nishikawa M, Behrndt M, Heisenberg C-PJ, Julicher F, Grill S. Determining
    physical properties of the cell cortex. <i>Biophysical Journal</i>. 2016;110(6):1421-1429.
    doi:<a href="https://doi.org/10.1016/j.bpj.2016.02.013">10.1016/j.bpj.2016.02.013</a>
  apa: Saha, A., Nishikawa, M., Behrndt, M., Heisenberg, C.-P. J., Julicher, F., &#38;
    Grill, S. (2016). Determining physical properties of the cell cortex. <i>Biophysical
    Journal</i>. Biophysical Society. <a href="https://doi.org/10.1016/j.bpj.2016.02.013">https://doi.org/10.1016/j.bpj.2016.02.013</a>
  chicago: Saha, Arnab, Masatoshi Nishikawa, Martin Behrndt, Carl-Philipp J Heisenberg,
    Frank Julicher, and Stephan Grill. “Determining Physical Properties of the Cell
    Cortex.” <i>Biophysical Journal</i>. Biophysical Society, 2016. <a href="https://doi.org/10.1016/j.bpj.2016.02.013">https://doi.org/10.1016/j.bpj.2016.02.013</a>.
  ieee: A. Saha, M. Nishikawa, M. Behrndt, C.-P. J. Heisenberg, F. Julicher, and S.
    Grill, “Determining physical properties of the cell cortex,” <i>Biophysical Journal</i>,
    vol. 110, no. 6. Biophysical Society, pp. 1421–1429, 2016.
  ista: Saha A, Nishikawa M, Behrndt M, Heisenberg C-PJ, Julicher F, Grill S. 2016.
    Determining physical properties of the cell cortex. Biophysical Journal. 110(6),
    1421–1429.
  mla: Saha, Arnab, et al. “Determining Physical Properties of the Cell Cortex.” <i>Biophysical
    Journal</i>, vol. 110, no. 6, Biophysical Society, 2016, pp. 1421–29, doi:<a href="https://doi.org/10.1016/j.bpj.2016.02.013">10.1016/j.bpj.2016.02.013</a>.
  short: A. Saha, M. Nishikawa, M. Behrndt, C.-P.J. Heisenberg, F. Julicher, S. Grill,
    Biophysical Journal 110 (2016) 1421–1429.
date_created: 2018-12-11T11:50:56Z
date_published: 2016-03-29T00:00:00Z
date_updated: 2021-01-12T06:49:23Z
day: '29'
ddc:
- '572'
- '576'
department:
- _id: CaHe
doi: 10.1016/j.bpj.2016.02.013
file:
- access_level: open_access
  checksum: c408cf2e25a25c8d711cffea524bda55
  content_type: application/pdf
  creator: system
  date_created: 2018-12-12T10:10:54Z
  date_updated: 2020-07-14T12:44:41Z
  file_id: '4845'
  file_name: IST-2016-706-v1+1_1-s2.0-S0006349516001582-main.pdf
  file_size: 1965645
  relation: main_file
file_date_updated: 2020-07-14T12:44:41Z
has_accepted_license: '1'
intvolume: '       110'
issue: '6'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-nd/4.0/
month: '03'
oa: 1
oa_version: Published Version
page: 1421 - 1429
project:
- _id: 252ABD0A-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: I 930-B20
  name: Control of Epithelial Cell Layer Spreading in Zebrafish
publication: Biophysical Journal
publication_status: published
publisher: Biophysical Society
publist_id: '6079'
pubrep_id: '706'
quality_controlled: '1'
scopus_import: 1
status: public
title: Determining physical properties of the cell cortex
tmp:
  image: /images/cc_by_nc_nd.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
    (CC BY-NC-ND 4.0)
  short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 110
year: '2016'
...
---
_id: '2282'
abstract:
- lang: eng
  text: Epithelial spreading is a common and fundamental aspect of various developmental
    and disease-related processes such as epithelial closure and wound healing. A
    key challenge for epithelial tissues undergoing spreading is to increase their
    surface area without disrupting epithelial integrity. Here we show that orienting
    cell divisions by tension constitutes an efficient mechanism by which the enveloping
    cell layer (EVL) releases anisotropic tension while undergoing spreading during
    zebrafish epiboly. The control of EVL cell-division orientation by tension involves
    cell elongation and requires myosin II activity to align the mitotic spindle with
    the main tension axis. We also found that in the absence of tension-oriented cell
    divisions and in the presence of increased tissue tension, EVL cells undergo ectopic
    fusions, suggesting that the reduction of tension anisotropy by oriented cell
    divisions is required to prevent EVL cells from fusing. We conclude that cell-division
    orientation by tension constitutes a key mechanism for limiting tension anisotropy
    and thus promoting tissue spreading during EVL epiboly.
acknowledged_ssus:
- _id: PreCl
- _id: Bio
acknowledgement: 'This work was supported by the IST Austria and MPI-CBG '
author:
- first_name: Pedro
  full_name: Campinho, Pedro
  id: 3AFBBC42-F248-11E8-B48F-1D18A9856A87
  last_name: Campinho
  orcid: 0000-0002-8526-5416
- first_name: Martin
  full_name: Behrndt, Martin
  id: 3ECECA3A-F248-11E8-B48F-1D18A9856A87
  last_name: Behrndt
- first_name: Jonas
  full_name: Ranft, Jonas
  last_name: Ranft
- first_name: Thomas
  full_name: Risler, Thomas
  last_name: Risler
- first_name: Nicolas
  full_name: Minc, Nicolas
  last_name: Minc
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
citation:
  ama: Campinho P, Behrndt M, Ranft J, Risler T, Minc N, Heisenberg C-PJ. Tension-oriented
    cell divisions limit anisotropic tissue tension in epithelial spreading during
    zebrafish epiboly. <i>Nature Cell Biology</i>. 2013;15:1405-1414. doi:<a href="https://doi.org/10.1038/ncb2869">10.1038/ncb2869</a>
  apa: Campinho, P., Behrndt, M., Ranft, J., Risler, T., Minc, N., &#38; Heisenberg,
    C.-P. J. (2013). Tension-oriented cell divisions limit anisotropic tissue tension
    in epithelial spreading during zebrafish epiboly. <i>Nature Cell Biology</i>.
    Nature Publishing Group. <a href="https://doi.org/10.1038/ncb2869">https://doi.org/10.1038/ncb2869</a>
  chicago: Campinho, Pedro, Martin Behrndt, Jonas Ranft, Thomas Risler, Nicolas Minc,
    and Carl-Philipp J Heisenberg. “Tension-Oriented Cell Divisions Limit Anisotropic
    Tissue Tension in Epithelial Spreading during Zebrafish Epiboly.” <i>Nature Cell
    Biology</i>. Nature Publishing Group, 2013. <a href="https://doi.org/10.1038/ncb2869">https://doi.org/10.1038/ncb2869</a>.
  ieee: P. Campinho, M. Behrndt, J. Ranft, T. Risler, N. Minc, and C.-P. J. Heisenberg,
    “Tension-oriented cell divisions limit anisotropic tissue tension in epithelial
    spreading during zebrafish epiboly,” <i>Nature Cell Biology</i>, vol. 15. Nature
    Publishing Group, pp. 1405–1414, 2013.
  ista: Campinho P, Behrndt M, Ranft J, Risler T, Minc N, Heisenberg C-PJ. 2013. Tension-oriented
    cell divisions limit anisotropic tissue tension in epithelial spreading during
    zebrafish epiboly. Nature Cell Biology. 15, 1405–1414.
  mla: Campinho, Pedro, et al. “Tension-Oriented Cell Divisions Limit Anisotropic
    Tissue Tension in Epithelial Spreading during Zebrafish Epiboly.” <i>Nature Cell
    Biology</i>, vol. 15, Nature Publishing Group, 2013, pp. 1405–14, doi:<a href="https://doi.org/10.1038/ncb2869">10.1038/ncb2869</a>.
  short: P. Campinho, M. Behrndt, J. Ranft, T. Risler, N. Minc, C.-P.J. Heisenberg,
    Nature Cell Biology 15 (2013) 1405–1414.
date_created: 2018-12-11T11:56:45Z
date_published: 2013-11-10T00:00:00Z
date_updated: 2023-02-21T17:02:44Z
day: '10'
department:
- _id: CaHe
doi: 10.1038/ncb2869
intvolume: '        15'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: http://hal.upmc.fr/hal-00983313/
month: '11'
oa: 1
oa_version: Submitted Version
page: 1405 - 1414
project:
- _id: 252ABD0A-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: I 930-B20
  name: Control of Epithelial Cell Layer Spreading in Zebrafish
publication: Nature Cell Biology
publication_status: published
publisher: Nature Publishing Group
publist_id: '4652'
quality_controlled: '1'
related_material:
  record:
  - id: '1403'
    relation: dissertation_contains
    status: public
scopus_import: 1
status: public
title: Tension-oriented cell divisions limit anisotropic tissue tension in epithelial
  spreading during zebrafish epiboly
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 15
year: '2013'
...
---
_id: '2950'
abstract:
- lang: eng
  text: Contractile actomyosin rings drive various fundamental morphogenetic processes
    ranging from cytokinesis to wound healing. Actomyosin rings are generally thought
    to function by circumferential contraction. Here, we show that the spreading of
    the enveloping cell layer (EVL) over the yolk cell during zebrafish gastrulation
    is driven by a contractile actomyosin ring. In contrast to previous suggestions,
    we find that this ring functions not only by circumferential contraction but also
    by a flow-friction mechanism. This generates a pulling force through resistance
    against retrograde actomyosin flow. EVL spreading proceeds normally in situations
    where circumferential contraction is unproductive, indicating that the flow-friction
    mechanism is sufficient. Thus, actomyosin rings can function in epithelial morphogenesis
    through a combination of cable-constriction and flow-friction mechanisms.
acknowledged_ssus:
- _id: SSU
author:
- first_name: Martin
  full_name: Behrndt, Martin
  id: 3ECECA3A-F248-11E8-B48F-1D18A9856A87
  last_name: Behrndt
- first_name: Guillaume
  full_name: Salbreux, Guillaume
  last_name: Salbreux
- first_name: Pedro
  full_name: Campinho, Pedro
  id: 3AFBBC42-F248-11E8-B48F-1D18A9856A87
  last_name: Campinho
  orcid: 0000-0002-8526-5416
- first_name: Robert
  full_name: Hauschild, Robert
  id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
  last_name: Hauschild
  orcid: 0000-0001-9843-3522
- first_name: Felix
  full_name: Oswald, Felix
  last_name: Oswald
- first_name: Julia
  full_name: Roensch, Julia
  id: 4220E59C-F248-11E8-B48F-1D18A9856A87
  last_name: Roensch
- first_name: Stephan
  full_name: Grill, Stephan
  last_name: Grill
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
citation:
  ama: Behrndt M, Salbreux G, Campinho P, et al. Forces driving epithelial spreading
    in zebrafish gastrulation. <i>Science</i>. 2012;338(6104):257-260. doi:<a href="https://doi.org/10.1126/science.1224143">10.1126/science.1224143</a>
  apa: Behrndt, M., Salbreux, G., Campinho, P., Hauschild, R., Oswald, F., Roensch,
    J., … Heisenberg, C.-P. J. (2012). Forces driving epithelial spreading in zebrafish
    gastrulation. <i>Science</i>. American Association for the Advancement of Science.
    <a href="https://doi.org/10.1126/science.1224143">https://doi.org/10.1126/science.1224143</a>
  chicago: Behrndt, Martin, Guillaume Salbreux, Pedro Campinho, Robert Hauschild,
    Felix Oswald, Julia Roensch, Stephan Grill, and Carl-Philipp J Heisenberg. “Forces
    Driving Epithelial Spreading in Zebrafish Gastrulation.” <i>Science</i>. American
    Association for the Advancement of Science, 2012. <a href="https://doi.org/10.1126/science.1224143">https://doi.org/10.1126/science.1224143</a>.
  ieee: M. Behrndt <i>et al.</i>, “Forces driving epithelial spreading in zebrafish
    gastrulation,” <i>Science</i>, vol. 338, no. 6104. American Association for the
    Advancement of Science, pp. 257–260, 2012.
  ista: Behrndt M, Salbreux G, Campinho P, Hauschild R, Oswald F, Roensch J, Grill
    S, Heisenberg C-PJ. 2012. Forces driving epithelial spreading in zebrafish gastrulation.
    Science. 338(6104), 257–260.
  mla: Behrndt, Martin, et al. “Forces Driving Epithelial Spreading in Zebrafish Gastrulation.”
    <i>Science</i>, vol. 338, no. 6104, American Association for the Advancement of
    Science, 2012, pp. 257–60, doi:<a href="https://doi.org/10.1126/science.1224143">10.1126/science.1224143</a>.
  short: M. Behrndt, G. Salbreux, P. Campinho, R. Hauschild, F. Oswald, J. Roensch,
    S. Grill, C.-P.J. Heisenberg, Science 338 (2012) 257–260.
date_created: 2018-12-11T12:00:30Z
date_published: 2012-10-12T00:00:00Z
date_updated: 2023-02-21T17:02:44Z
day: '12'
department:
- _id: CaHe
- _id: Bio
doi: 10.1126/science.1224143
intvolume: '       338'
issue: '6104'
language:
- iso: eng
month: '10'
oa_version: None
page: 257 - 260
project:
- _id: 252ABD0A-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: I 930-B20
  name: Control of Epithelial Cell Layer Spreading in Zebrafish
publication: Science
publication_status: published
publisher: American Association for the Advancement of Science
publist_id: '3778'
quality_controlled: '1'
related_material:
  record:
  - id: '1403'
    relation: dissertation_contains
    status: public
scopus_import: 1
status: public
title: Forces driving epithelial spreading in zebrafish gastrulation
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 338
year: '2012'
...