---
_id: '1100'
abstract:
- lang: eng
  text: During metazoan development, the temporal pattern of morphogen signaling is
    critical for organizing cell fates in space and time. Yet, tools for temporally
    controlling morphogen signaling within the embryo are still scarce. Here, we developed
    a photoactivatable Nodal receptor to determine how the temporal pattern of Nodal
    signaling affects cell fate specification during zebrafish gastrulation. By using
    this receptor to manipulate the duration of Nodal signaling in vivo by light,
    we show that extended Nodal signaling within the organizer promotes prechordal
    plate specification and suppresses endoderm differentiation. Endoderm differentiation
    is suppressed by extended Nodal signaling inducing expression of the transcriptional
    repressor goosecoid (gsc) in prechordal plate progenitors, which in turn restrains
    Nodal signaling from upregulating the endoderm differentiation gene sox17 within
    these cells. Thus, optogenetic manipulation of Nodal signaling identifies a critical
    role of Nodal signaling duration for organizer cell fate specification during
    gastrulation.
acknowledged_ssus:
- _id: SSU
acknowledgement: 'We are grateful to members of the C.-P.H. and H.J. labs for discussions,
  R. Hauschild and the different Scientific Service Units at IST Austria for technical
  help, M. Dravecka for performing initial experiments, A. Schier for reading an earlier
  version of the manuscript, K.W. Rogers for technical help, and C. Hill, A. Bruce,
  and L. Solnica-Krezel for sending plasmids. This work was supported by grants from
  the Austrian Science Foundation (FWF): (T560-B17) and (I 812-B12) to V.R. and C.-P.H.,
  and from the European Union (EU FP7): (6275) to H.J. A.I.-P. is supported by a Ramon
  Areces fellowship.'
author:
- first_name: Keisuke
  full_name: Sako, Keisuke
  id: 3BED66BE-F248-11E8-B48F-1D18A9856A87
  last_name: Sako
  orcid: 0000-0002-6453-8075
- first_name: Saurabh
  full_name: Pradhan, Saurabh
  last_name: Pradhan
- first_name: Vanessa
  full_name: Barone, Vanessa
  id: 419EECCC-F248-11E8-B48F-1D18A9856A87
  last_name: Barone
  orcid: 0000-0003-2676-3367
- first_name: Álvaro
  full_name: Inglés Prieto, Álvaro
  id: 2A9DB292-F248-11E8-B48F-1D18A9856A87
  last_name: Inglés Prieto
  orcid: 0000-0002-5409-8571
- first_name: Patrick
  full_name: Mueller, Patrick
  last_name: Mueller
- first_name: Verena
  full_name: Ruprecht, Verena
  id: 4D71A03A-F248-11E8-B48F-1D18A9856A87
  last_name: Ruprecht
  orcid: 0000-0003-4088-8633
- first_name: Daniel
  full_name: Capek, Daniel
  id: 31C42484-F248-11E8-B48F-1D18A9856A87
  last_name: Capek
  orcid: 0000-0001-5199-9940
- first_name: Sanjeev
  full_name: Galande, Sanjeev
  last_name: Galande
- first_name: Harald L
  full_name: Janovjak, Harald L
  id: 33BA6C30-F248-11E8-B48F-1D18A9856A87
  last_name: Janovjak
  orcid: 0000-0002-8023-9315
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
citation:
  ama: Sako K, Pradhan S, Barone V, et al. Optogenetic control of nodal signaling
    reveals a temporal pattern of nodal signaling regulating cell fate specification
    during gastrulation. <i>Cell Reports</i>. 2016;16(3):866-877. doi:<a href="https://doi.org/10.1016/j.celrep.2016.06.036">10.1016/j.celrep.2016.06.036</a>
  apa: Sako, K., Pradhan, S., Barone, V., Inglés Prieto, Á., Mueller, P., Ruprecht,
    V., … Heisenberg, C.-P. J. (2016). Optogenetic control of nodal signaling reveals
    a temporal pattern of nodal signaling regulating cell fate specification during
    gastrulation. <i>Cell Reports</i>. Cell Press. <a href="https://doi.org/10.1016/j.celrep.2016.06.036">https://doi.org/10.1016/j.celrep.2016.06.036</a>
  chicago: Sako, Keisuke, Saurabh Pradhan, Vanessa Barone, Álvaro Inglés Prieto, Patrick
    Mueller, Verena Ruprecht, Daniel Capek, Sanjeev Galande, Harald L Janovjak, and
    Carl-Philipp J Heisenberg. “Optogenetic Control of Nodal Signaling Reveals a Temporal
    Pattern of Nodal Signaling Regulating Cell Fate Specification during Gastrulation.”
    <i>Cell Reports</i>. Cell Press, 2016. <a href="https://doi.org/10.1016/j.celrep.2016.06.036">https://doi.org/10.1016/j.celrep.2016.06.036</a>.
  ieee: K. Sako <i>et al.</i>, “Optogenetic control of nodal signaling reveals a temporal
    pattern of nodal signaling regulating cell fate specification during gastrulation,”
    <i>Cell Reports</i>, vol. 16, no. 3. Cell Press, pp. 866–877, 2016.
  ista: Sako K, Pradhan S, Barone V, Inglés Prieto Á, Mueller P, Ruprecht V, Capek
    D, Galande S, Janovjak HL, Heisenberg C-PJ. 2016. Optogenetic control of nodal
    signaling reveals a temporal pattern of nodal signaling regulating cell fate specification
    during gastrulation. Cell Reports. 16(3), 866–877.
  mla: Sako, Keisuke, et al. “Optogenetic Control of Nodal Signaling Reveals a Temporal
    Pattern of Nodal Signaling Regulating Cell Fate Specification during Gastrulation.”
    <i>Cell Reports</i>, vol. 16, no. 3, Cell Press, 2016, pp. 866–77, doi:<a href="https://doi.org/10.1016/j.celrep.2016.06.036">10.1016/j.celrep.2016.06.036</a>.
  short: K. Sako, S. Pradhan, V. Barone, Á. Inglés Prieto, P. Mueller, V. Ruprecht,
    D. Capek, S. Galande, H.L. Janovjak, C.-P.J. Heisenberg, Cell Reports 16 (2016)
    866–877.
date_created: 2018-12-11T11:50:08Z
date_published: 2016-07-19T00:00:00Z
date_updated: 2024-03-25T23:30:13Z
day: '19'
ddc:
- '570'
- '576'
department:
- _id: CaHe
- _id: HaJa
doi: 10.1016/j.celrep.2016.06.036
ec_funded: 1
file:
- access_level: open_access
  content_type: application/pdf
  creator: system
  date_created: 2018-12-12T10:11:04Z
  date_updated: 2018-12-12T10:11:04Z
  file_id: '4857'
  file_name: IST-2017-754-v1+1_1-s2.0-S2211124716307768-main.pdf
  file_size: 3921947
  relation: main_file
file_date_updated: 2018-12-12T10:11:04Z
has_accepted_license: '1'
intvolume: '        16'
issue: '3'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '07'
oa: 1
oa_version: Published Version
page: 866 - 877
project:
- _id: 2529486C-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: T 560-B17
  name: Cell- and Tissue Mechanics in Zebrafish Germ Layer Formation
- _id: 2527D5CC-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: I 812-B12
  name: Cell Cortex and Germ Layer Formation in Zebrafish Gastrulation
- _id: 25548C20-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '303564'
  name: Microbial Ion Channels for Synthetic Neurobiology
publication: Cell Reports
publication_status: published
publisher: Cell Press
publist_id: '6275'
pubrep_id: '754'
quality_controlled: '1'
related_material:
  record:
  - id: '961'
    relation: dissertation_contains
    status: public
  - id: '50'
    relation: dissertation_contains
    status: public
scopus_import: 1
status: public
title: Optogenetic control of nodal signaling reveals a temporal pattern of nodal
  signaling regulating cell fate specification during gastrulation
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 16
year: '2016'
...
---
_id: '1537'
abstract:
- lang: eng
  text: 3D amoeboid cell migration is central to many developmental and disease-related
    processes such as cancer metastasis. Here, we identify a unique prototypic amoeboid
    cell migration mode in early zebrafish embryos, termed stable-bleb migration.
    Stable-bleb cells display an invariant polarized balloon-like shape with exceptional
    migration speed and persistence. Progenitor cells can be reversibly transformed
    into stable-bleb cells irrespective of their primary fate and motile characteristics
    by increasing myosin II activity through biochemical or mechanical stimuli. Using
    a combination of theory and experiments, we show that, in stable-bleb cells, cortical
    contractility fluctuations trigger a stochastic switch into amoeboid motility,
    and a positive feedback between cortical flows and gradients in contractility
    maintains stable-bleb cell polarization. We further show that rearward cortical
    flows drive stable-bleb cell migration in various adhesive and non-adhesive environments,
    unraveling a highly versatile amoeboid migration phenotype.
acknowledged_ssus:
- _id: SSU
acknowledgement: 'We would like to thank R. Hausschild and E. Papusheva for technical
  assistance and the service facilities at the IST Austria for continuous support.
  The caRhoA plasmid was a kind gift of T. Kudoh and A. Takesono. We thank M. Piel
  and E. Paluch for exchanging unpublished data. '
author:
- first_name: Verena
  full_name: Ruprecht, Verena
  id: 4D71A03A-F248-11E8-B48F-1D18A9856A87
  last_name: Ruprecht
  orcid: 0000-0003-4088-8633
- first_name: Stefan
  full_name: Wieser, Stefan
  id: 355AA5A0-F248-11E8-B48F-1D18A9856A87
  last_name: Wieser
  orcid: 0000-0002-2670-2217
- first_name: Andrew
  full_name: Callan Jones, Andrew
  last_name: Callan Jones
- first_name: Michael
  full_name: Smutny, Michael
  id: 3FE6E4E8-F248-11E8-B48F-1D18A9856A87
  last_name: Smutny
  orcid: 0000-0002-5920-9090
- first_name: Hitoshi
  full_name: Morita, Hitoshi
  id: 4C6E54C6-F248-11E8-B48F-1D18A9856A87
  last_name: Morita
- first_name: Keisuke
  full_name: Sako, Keisuke
  id: 3BED66BE-F248-11E8-B48F-1D18A9856A87
  last_name: Sako
  orcid: 0000-0002-6453-8075
- first_name: Vanessa
  full_name: Barone, Vanessa
  id: 419EECCC-F248-11E8-B48F-1D18A9856A87
  last_name: Barone
  orcid: 0000-0003-2676-3367
- first_name: Monika
  full_name: Ritsch Marte, Monika
  last_name: Ritsch Marte
- first_name: Michael K
  full_name: Sixt, Michael K
  id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
  last_name: Sixt
  orcid: 0000-0002-6620-9179
- first_name: Raphaël
  full_name: Voituriez, Raphaël
  last_name: Voituriez
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
citation:
  ama: Ruprecht V, Wieser S, Callan Jones A, et al. Cortical contractility triggers
    a stochastic switch to fast amoeboid cell motility. <i>Cell</i>. 2015;160(4):673-685.
    doi:<a href="https://doi.org/10.1016/j.cell.2015.01.008">10.1016/j.cell.2015.01.008</a>
  apa: Ruprecht, V., Wieser, S., Callan Jones, A., Smutny, M., Morita, H., Sako, K.,
    … Heisenberg, C.-P. J. (2015). Cortical contractility triggers a stochastic switch
    to fast amoeboid cell motility. <i>Cell</i>. Cell Press. <a href="https://doi.org/10.1016/j.cell.2015.01.008">https://doi.org/10.1016/j.cell.2015.01.008</a>
  chicago: Ruprecht, Verena, Stefan Wieser, Andrew Callan Jones, Michael Smutny, Hitoshi
    Morita, Keisuke Sako, Vanessa Barone, et al. “Cortical Contractility Triggers
    a Stochastic Switch to Fast Amoeboid Cell Motility.” <i>Cell</i>. Cell Press,
    2015. <a href="https://doi.org/10.1016/j.cell.2015.01.008">https://doi.org/10.1016/j.cell.2015.01.008</a>.
  ieee: V. Ruprecht <i>et al.</i>, “Cortical contractility triggers a stochastic switch
    to fast amoeboid cell motility,” <i>Cell</i>, vol. 160, no. 4. Cell Press, pp.
    673–685, 2015.
  ista: Ruprecht V, Wieser S, Callan Jones A, Smutny M, Morita H, Sako K, Barone V,
    Ritsch Marte M, Sixt MK, Voituriez R, Heisenberg C-PJ. 2015. Cortical contractility
    triggers a stochastic switch to fast amoeboid cell motility. Cell. 160(4), 673–685.
  mla: Ruprecht, Verena, et al. “Cortical Contractility Triggers a Stochastic Switch
    to Fast Amoeboid Cell Motility.” <i>Cell</i>, vol. 160, no. 4, Cell Press, 2015,
    pp. 673–85, doi:<a href="https://doi.org/10.1016/j.cell.2015.01.008">10.1016/j.cell.2015.01.008</a>.
  short: V. Ruprecht, S. Wieser, A. Callan Jones, M. Smutny, H. Morita, K. Sako, V.
    Barone, M. Ritsch Marte, M.K. Sixt, R. Voituriez, C.-P.J. Heisenberg, Cell 160
    (2015) 673–685.
date_created: 2018-12-11T11:52:35Z
date_published: 2015-02-12T00:00:00Z
date_updated: 2023-09-07T12:05:08Z
day: '12'
ddc:
- '570'
department:
- _id: CaHe
- _id: MiSi
doi: 10.1016/j.cell.2015.01.008
file:
- access_level: open_access
  checksum: 228d3edf40627d897b3875088a0ac51f
  content_type: application/pdf
  creator: system
  date_created: 2018-12-12T10:13:21Z
  date_updated: 2020-07-14T12:45:01Z
  file_id: '5003'
  file_name: IST-2016-484-v1+1_1-s2.0-S0092867415000094-main.pdf
  file_size: 4362653
  relation: main_file
file_date_updated: 2020-07-14T12:45:01Z
has_accepted_license: '1'
intvolume: '       160'
issue: '4'
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
page: 673 - 685
project:
- _id: 2529486C-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: T 560-B17
  name: Cell- and Tissue Mechanics in Zebrafish Germ Layer Formation
- _id: 2527D5CC-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: I 812-B12
  name: Cell Cortex and Germ Layer Formation in Zebrafish Gastrulation
publication: Cell
publication_status: published
publisher: Cell Press
publist_id: '5634'
pubrep_id: '484'
quality_controlled: '1'
related_material:
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  - id: '961'
    relation: dissertation_contains
    status: public
scopus_import: 1
status: public
title: Cortical contractility triggers a stochastic switch to fast amoeboid cell motility
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 160
year: '2015'
...
---
_id: '2884'
author:
- first_name: Jean-Léon
  full_name: Maître, Jean-Léon
  id: 48F1E0D8-F248-11E8-B48F-1D18A9856A87
  last_name: Maître
  orcid: 0000-0002-3688-1474
- first_name: Hélène
  full_name: Berthoumieux, Hélène
  last_name: Berthoumieux
- first_name: Gabriel
  full_name: Krens, Gabriel
  id: 2B819732-F248-11E8-B48F-1D18A9856A87
  last_name: Krens
  orcid: 0000-0003-4761-5996
- first_name: Guillaume
  full_name: Salbreux, Guillaume
  last_name: Salbreux
- first_name: Frank
  full_name: Julicher, Frank
  last_name: Julicher
- first_name: Ewa
  full_name: Paluch, Ewa
  last_name: Paluch
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
citation:
  ama: Maître J-L, Berthoumieux H, Krens G, et al. Cell adhesion mechanics of zebrafish
    gastrulation. <i>Medecine Sciences</i>. 2013;29(2):147-150. doi:<a href="https://doi.org/10.1051/medsci/2013292011">10.1051/medsci/2013292011</a>
  apa: Maître, J.-L., Berthoumieux, H., Krens, G., Salbreux, G., Julicher, F., Paluch,
    E., &#38; Heisenberg, C.-P. J. (2013). Cell adhesion mechanics of zebrafish gastrulation.
    <i>Medecine Sciences</i>. Éditions Médicales et Scientifiques. <a href="https://doi.org/10.1051/medsci/2013292011">https://doi.org/10.1051/medsci/2013292011</a>
  chicago: Maître, Jean-Léon, Hélène Berthoumieux, Gabriel Krens, Guillaume Salbreux,
    Frank Julicher, Ewa Paluch, and Carl-Philipp J Heisenberg. “Cell Adhesion Mechanics
    of Zebrafish Gastrulation.” <i>Medecine Sciences</i>. Éditions Médicales et Scientifiques,
    2013. <a href="https://doi.org/10.1051/medsci/2013292011">https://doi.org/10.1051/medsci/2013292011</a>.
  ieee: J.-L. Maître <i>et al.</i>, “Cell adhesion mechanics of zebrafish gastrulation,”
    <i>Medecine Sciences</i>, vol. 29, no. 2. Éditions Médicales et Scientifiques,
    pp. 147–150, 2013.
  ista: Maître J-L, Berthoumieux H, Krens G, Salbreux G, Julicher F, Paluch E, Heisenberg
    C-PJ. 2013. Cell adhesion mechanics of zebrafish gastrulation. Medecine Sciences.
    29(2), 147–150.
  mla: Maître, Jean-Léon, et al. “Cell Adhesion Mechanics of Zebrafish Gastrulation.”
    <i>Medecine Sciences</i>, vol. 29, no. 2, Éditions Médicales et Scientifiques,
    2013, pp. 147–50, doi:<a href="https://doi.org/10.1051/medsci/2013292011">10.1051/medsci/2013292011</a>.
  short: J.-L. Maître, H. Berthoumieux, G. Krens, G. Salbreux, F. Julicher, E. Paluch,
    C.-P.J. Heisenberg, Medecine Sciences 29 (2013) 147–150.
date_created: 2018-12-11T12:00:08Z
date_published: 2013-02-01T00:00:00Z
date_updated: 2021-01-12T07:00:28Z
day: '01'
department:
- _id: CaHe
doi: 10.1051/medsci/2013292011
intvolume: '        29'
issue: '2'
language:
- iso: eng
month: '02'
oa_version: None
page: 147 - 150
project:
- _id: 252064B8-B435-11E9-9278-68D0E5697425
  grant_number: HE_3231/6-1
  name: Analysis of the Formation and Function of Different Cell Protusion Types During
    Cell Migration in Vivo
- _id: 2527D5CC-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: I 812-B12
  name: Cell Cortex and Germ Layer Formation in Zebrafish Gastrulation
publication: Medecine Sciences
publication_status: published
publisher: Éditions Médicales et Scientifiques
publist_id: '3877'
quality_controlled: '1'
scopus_import: 1
status: public
title: Cell adhesion mechanics of zebrafish gastrulation
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 29
year: '2013'
...