---
_id: '10890'
abstract:
- lang: eng
  text: Upon the arrival of action potentials at nerve terminals, neurotransmitters
    are released from synaptic vesicles (SVs) by exocytosis. CaV2.1, 2.2, and 2.3
    are the major subunits of the voltage-gated calcium channel (VGCC) responsible
    for increasing intraterminal calcium levels and triggering SV exocytosis in the
    central nervous system (CNS) synapses. The two-dimensional analysis of CaV2 distributions
    using sodium dodecyl sulfate (SDS)-digested freeze-fracture replica labeling (SDS-FRL)
    has revealed their numbers, densities, and nanoscale clustering patterns in individual
    presynaptic active zones. The variation in these properties affects the coupling
    of VGCCs with calcium sensors on SVs, synaptic efficacy, and temporal precision
    of transmission. In this study, we summarize how the morphological parameters
    of CaV2 distribution obtained using SDS-FRL differ depending on the different
    types of synapses and could correspond to functional properties in synaptic transmission.
acknowledgement: "This work was supported by the European Research Council advanced
  grant No. 694539 and the joint German-Austrian DFG and FWF project SYNABS (FWF:
  I-4638-B) to RS.\r\nThe authors thank Walter Kaufmann for his critical comments
  on the manuscript."
article_number: '846615'
article_processing_charge: No
article_type: original
author:
- first_name: Kohgaku
  full_name: Eguchi, Kohgaku
  id: 2B7846DC-F248-11E8-B48F-1D18A9856A87
  last_name: Eguchi
  orcid: 0000-0002-6170-2546
- first_name: Jacqueline-Claire
  full_name: Montanaro-Punzengruber, Jacqueline-Claire
  id: 3786AB44-F248-11E8-B48F-1D18A9856A87
  last_name: Montanaro-Punzengruber
- first_name: Elodie
  full_name: Le Monnier, Elodie
  id: 3B59276A-F248-11E8-B48F-1D18A9856A87
  last_name: Le Monnier
- first_name: Ryuichi
  full_name: Shigemoto, Ryuichi
  id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
  last_name: Shigemoto
  orcid: 0000-0001-8761-9444
citation:
  ama: Eguchi K, Montanaro-Punzengruber J-C, Le Monnier E, Shigemoto R. The number
    and distinct clustering patterns of voltage-gated Calcium channels in nerve terminals.
    <i>Frontiers in Neuroanatomy</i>. 2022;16. doi:<a href="https://doi.org/10.3389/fnana.2022.846615">10.3389/fnana.2022.846615</a>
  apa: Eguchi, K., Montanaro-Punzengruber, J.-C., Le Monnier, E., &#38; Shigemoto,
    R. (2022). The number and distinct clustering patterns of voltage-gated Calcium
    channels in nerve terminals. <i>Frontiers in Neuroanatomy</i>. Frontiers. <a href="https://doi.org/10.3389/fnana.2022.846615">https://doi.org/10.3389/fnana.2022.846615</a>
  chicago: Eguchi, Kohgaku, Jacqueline-Claire Montanaro-Punzengruber, Elodie Le Monnier,
    and Ryuichi Shigemoto. “The Number and Distinct Clustering Patterns of Voltage-Gated
    Calcium Channels in Nerve Terminals.” <i>Frontiers in Neuroanatomy</i>. Frontiers,
    2022. <a href="https://doi.org/10.3389/fnana.2022.846615">https://doi.org/10.3389/fnana.2022.846615</a>.
  ieee: K. Eguchi, J.-C. Montanaro-Punzengruber, E. Le Monnier, and R. Shigemoto,
    “The number and distinct clustering patterns of voltage-gated Calcium channels
    in nerve terminals,” <i>Frontiers in Neuroanatomy</i>, vol. 16. Frontiers, 2022.
  ista: Eguchi K, Montanaro-Punzengruber J-C, Le Monnier E, Shigemoto R. 2022. The
    number and distinct clustering patterns of voltage-gated Calcium channels in nerve
    terminals. Frontiers in Neuroanatomy. 16, 846615.
  mla: Eguchi, Kohgaku, et al. “The Number and Distinct Clustering Patterns of Voltage-Gated
    Calcium Channels in Nerve Terminals.” <i>Frontiers in Neuroanatomy</i>, vol. 16,
    846615, Frontiers, 2022, doi:<a href="https://doi.org/10.3389/fnana.2022.846615">10.3389/fnana.2022.846615</a>.
  short: K. Eguchi, J.-C. Montanaro-Punzengruber, E. Le Monnier, R. Shigemoto, Frontiers
    in Neuroanatomy 16 (2022).
date_created: 2022-03-20T23:01:39Z
date_published: 2022-02-24T00:00:00Z
date_updated: 2024-10-29T07:57:26Z
day: '24'
ddc:
- '570'
department:
- _id: RySh
doi: 10.3389/fnana.2022.846615
ec_funded: 1
external_id:
  isi:
  - '000766662700001'
  pmid:
  - '35280978'
file:
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  creator: dernst
  date_created: 2022-03-21T09:41:19Z
  date_updated: 2022-03-21T09:41:19Z
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file_date_updated: 2022-03-21T09:41:19Z
has_accepted_license: '1'
intvolume: '        16'
isi: 1
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 25CA28EA-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '694539'
  name: 'In situ analysis of single channel subunit composition in neurons: physiological
    implication in synaptic plasticity and behaviour'
- _id: 05970B30-7A3F-11EA-A408-12923DDC885E
  grant_number: I04638
  name: LGI1 antibody-induced pathophysiology in synapses
publication: Frontiers in Neuroanatomy
publication_identifier:
  eissn:
  - '16625129'
publication_status: published
publisher: Frontiers
quality_controlled: '1'
scopus_import: '1'
status: public
title: The number and distinct clustering patterns of voltage-gated Calcium channels
  in nerve terminals
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 16
year: '2022'
...