---
_id: '12875'
abstract:
- lang: eng
  text: The superior colliculus (SC) in the mammalian midbrain is essential for multisensory
    integration and is composed of a rich diversity of excitatory and inhibitory neurons
    and glia. However, the developmental principles directing the generation of SC
    cell-type diversity are not understood. Here, we pursued systematic cell lineage
    tracing in silico and in vivo, preserving full spatial information, using genetic
    mosaic analysis with double markers (MADM)-based clonal analysis with single-cell
    sequencing (MADM-CloneSeq). The analysis of clonally related cell lineages revealed
    that radial glial progenitors (RGPs) in SC are exceptionally multipotent. Individual
    resident RGPs have the capacity to produce all excitatory and inhibitory SC neuron
    types, even at the stage of terminal division. While individual clonal units show
    no pre-defined cellular composition, the establishment of appropriate relative
    proportions of distinct neuronal types occurs in a PTEN-dependent manner. Collectively,
    our findings provide an inaugural framework at the single-RGP/-cell level of the
    mammalian SC ontogeny.
acknowledged_ssus:
- _id: Bio
- _id: M-Shop
- _id: LifeSc
- _id: PreCl
acknowledgement: "We thank Liqun Luo for his continued support, for providing essential
  resources for generating Fzd10-CreER mice which were generated in his laboratory,
  and for comments on the manuscript; W. Zhong for providing Nestin-Cre transgenic
  mouse line for this study; A. Heger for mouse colony management; R. Beattie and
  T. Asenov for designing and producing components of acute slice recovery chamber
  for MADM-CloneSeq experiments; and K. Leopold, J. Rodarte and N. Amberg for initial
  experiments, technical support and/or assistance. This study was supported by the
  Scientific Service Units (SSU) of IST Austria through resources provided by the
  Imaging & Optics Facility (IOF), Laboratory Support Facility (LSF), Miba Machine
  Shop, and Pre-clinical Facility (PCF). G.C. received funding from European Commission
  (IST plus postdoctoral fellowship). This work was supported by ISTA institutional\r\nfunds;
  the Austrian Science Fund Special Research Programmes (FWF SFB F78 Neuro Stem Modulation)
  to S.H. "
article_processing_charge: Yes (via OA deal)
article_type: comment
author:
- first_name: Giselle T
  full_name: Cheung, Giselle T
  id: 471195F6-F248-11E8-B48F-1D18A9856A87
  last_name: Cheung
  orcid: 0000-0001-8457-2572
- first_name: Florian
  full_name: Pauler, Florian
  id: 48EA0138-F248-11E8-B48F-1D18A9856A87
  last_name: Pauler
  orcid: 0000-0002-7462-0048
- first_name: Peter
  full_name: Koppensteiner, Peter
  id: 3B8B25A8-F248-11E8-B48F-1D18A9856A87
  last_name: Koppensteiner
  orcid: 0000-0002-3509-1948
- first_name: Thomas
  full_name: Krausgruber, Thomas
  last_name: Krausgruber
- first_name: Carmen
  full_name: Streicher, Carmen
  id: 36BCB99C-F248-11E8-B48F-1D18A9856A87
  last_name: Streicher
- first_name: Martin
  full_name: Schrammel, Martin
  id: f13e7cae-e8bd-11ed-841a-96dedf69f46d
  last_name: Schrammel
- first_name: Natalie Y
  full_name: Özgen, Natalie Y
  id: e68ece33-f6e0-11ea-865d-ae1031dcc090
  last_name: Özgen
- first_name: Alexis
  full_name: Ivec, Alexis
  id: 1d144691-e8be-11ed-9b33-bdd3077fad4c
  last_name: Ivec
- first_name: Christoph
  full_name: Bock, Christoph
  last_name: Bock
- first_name: Ryuichi
  full_name: Shigemoto, Ryuichi
  id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
  last_name: Shigemoto
  orcid: 0000-0001-8761-9444
- first_name: Simon
  full_name: Hippenmeyer, Simon
  id: 37B36620-F248-11E8-B48F-1D18A9856A87
  last_name: Hippenmeyer
  orcid: 0000-0003-2279-1061
date_created: 2023-04-27T09:41:48Z
date_published: 2024-01-17T00:00:00Z
date_updated: 2025-05-14T09:39:37Z
day: '17'
ddc:
- '570'
department:
- _id: SiHi
- _id: RySh
doi: 10.1016/j.neuron.2023.11.009
external_id:
  pmid:
  - '38096816'
file:
- access_level: open_access
  checksum: 32b3788f7085cf44a84108d8faaff3ce
  content_type: application/pdf
  creator: dernst
  date_created: 2024-02-06T13:56:15Z
  date_updated: 2024-02-06T13:56:15Z
  file_id: '14944'
  file_name: 2024_Neuron_Cheung.pdf
  file_size: 5942467
  relation: main_file
  success: 1
file_date_updated: 2024-02-06T13:56:15Z
has_accepted_license: '1'
intvolume: '       112'
issue: '2'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
page: 230-246.e11
pmid: 1
project:
- _id: 059F6AB4-7A3F-11EA-A408-12923DDC885E
  grant_number: F07805
  name: Molecular Mechanisms of Neural Stem Cell Lineage Progression
publication: Neuron
publication_identifier:
  eisbn:
  - '1234995621'
  issn:
  - 0896-6273
  issnl:
  - 1234-5678
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
  link:
  - description: News on ISTA Website
    relation: press_release
    url: https://ista.ac.at/en/news/the-pedigree-of-brain-cells/
scopus_import: '1'
status: public
title: Multipotent progenitors instruct ontogeny of the superior colliculus
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 112
year: '2024'
...