---
_id: '13259'
abstract:
- lang: eng
  text: Plants can regenerate their bodies via de novo establishment of shoot apical
    meristems (SAMs) from pluripotent callus. Only a small fraction of callus cells
    is eventually specified into SAMs but the molecular mechanisms underlying fate
    specification remain obscure. The expression of WUSCHEL (WUS) is an early hallmark
    of SAM fate acquisition. Here, we show that a WUS paralog, WUSCHEL-RELATED HOMEOBOX
    13 (WOX13), negatively regulates SAM formation from callus in Arabidopsis thaliana.
    WOX13 promotes non-meristematic cell fate via transcriptional repression of WUS
    and other SAM regulators and activation of cell wall modifiers. Our Quartz-Seq2–based
    single cell transcriptome revealed that WOX13 plays key roles in determining cellular
    identity of callus cell population. We propose that reciprocal inhibition between
    WUS and WOX13 mediates critical cell fate determination in pluripotent cell population,
    which has a major impact on regeneration efficiency.
acknowledgement: Wethank Y.Iwayama, K.Ohtawa, K.Fukumoto,andN. Mataga (RIKENRRD) for
  technical assistance in Quartz-Seq2analyses; M. Mouri(RIKENCSRS)for technical support
  with plasmid construction and transactivation assay; Y. Ikeda (NAIST) for technical
  support with tissue culture; and A. Furuta for technical support in bulk RNA-seq
  analysis. We also thank the Single-cell Omics Laboratory for technical consultation
  in scRNA-seq analyses, the members of the Laboratory for Bioinformatics Research
  at the RIKEN Center for Biosystems Dynamics Research, and A. Matsushima and T. Ichikawa
  for IT infrastructure management. This work was supported by JSPS KAKENHI(17K15146,19H05670,20K06712,20H04894,20H05431,and
  22H04713 to M.I. and 20H03284 and 20H05911 to K.S.), by the JST FOREST Program (JPMJFR214H
  to M.I.), by The Naito Foundation to M.I.; by Takeda Science Foundation to M.I,and
  by the Shiseido Female Researcher Science Grant to M.I. This work was partially
  supported by RIKENE pigenome Control Program, Medical Research Center Initiative
  for High Depth Omics, and JST CREST(JPMJCR16G3and JPMJCR1926)to I.N.
article_processing_charge: Yes
article_type: original
author:
- first_name: Nao
  full_name: Ogura, Nao
  last_name: Ogura
- first_name: Yohei
  full_name: Sasagawa, Yohei
  last_name: Sasagawa
- first_name: Tasuku
  full_name: Ito, Tasuku
  id: d5a17a4a-e534-11eb-93ec-91fa2aa9bd57
  last_name: Ito
  orcid: 0000-0002-2482-9089
- first_name: Toshiaki
  full_name: Tameshige, Toshiaki
  last_name: Tameshige
- first_name: Satomi
  full_name: Kawai, Satomi
  last_name: Kawai
- first_name: Masaki
  full_name: Sano, Masaki
  last_name: Sano
- first_name: Yuki
  full_name: Doll, Yuki
  last_name: Doll
- first_name: Akira
  full_name: Iwase, Akira
  last_name: Iwase
- first_name: Ayako
  full_name: Kawamura, Ayako
  last_name: Kawamura
- first_name: Takamasa
  full_name: Suzuki, Takamasa
  last_name: Suzuki
- first_name: Itoshi
  full_name: Nikaido, Itoshi
  last_name: Nikaido
- first_name: Keiko
  full_name: Sugimoto, Keiko
  last_name: Sugimoto
- first_name: Momoko
  full_name: Ikeuchi, Momoko
  last_name: Ikeuchi
citation:
  ama: Ogura N, Sasagawa Y, Ito T, et al. WUSCHEL-RELATED HOMEOBOX 13 suppresses de
    novo shoot regeneration via cell fate control of pluripotent callus. <i>Science
    Advances</i>. 2023;9(27):eadg6983. doi:<a href="https://doi.org/10.1126/sciadv.adg6983">10.1126/sciadv.adg6983</a>
  apa: Ogura, N., Sasagawa, Y., Ito, T., Tameshige, T., Kawai, S., Sano, M., … Ikeuchi,
    M. (2023). WUSCHEL-RELATED HOMEOBOX 13 suppresses de novo shoot regeneration via
    cell fate control of pluripotent callus. <i>Science Advances</i>. American Association
    for the Advancement of Science. <a href="https://doi.org/10.1126/sciadv.adg6983">https://doi.org/10.1126/sciadv.adg6983</a>
  chicago: Ogura, Nao, Yohei Sasagawa, Tasuku Ito, Toshiaki Tameshige, Satomi Kawai,
    Masaki Sano, Yuki Doll, et al. “WUSCHEL-RELATED HOMEOBOX 13 Suppresses de Novo
    Shoot Regeneration via Cell Fate Control of Pluripotent Callus.” <i>Science Advances</i>.
    American Association for the Advancement of Science, 2023. <a href="https://doi.org/10.1126/sciadv.adg6983">https://doi.org/10.1126/sciadv.adg6983</a>.
  ieee: N. Ogura <i>et al.</i>, “WUSCHEL-RELATED HOMEOBOX 13 suppresses de novo shoot
    regeneration via cell fate control of pluripotent callus,” <i>Science Advances</i>,
    vol. 9, no. 27. American Association for the Advancement of Science, p. eadg6983,
    2023.
  ista: Ogura N, Sasagawa Y, Ito T, Tameshige T, Kawai S, Sano M, Doll Y, Iwase A,
    Kawamura A, Suzuki T, Nikaido I, Sugimoto K, Ikeuchi M. 2023. WUSCHEL-RELATED
    HOMEOBOX 13 suppresses de novo shoot regeneration via cell fate control of pluripotent
    callus. Science Advances. 9(27), eadg6983.
  mla: Ogura, Nao, et al. “WUSCHEL-RELATED HOMEOBOX 13 Suppresses de Novo Shoot Regeneration
    via Cell Fate Control of Pluripotent Callus.” <i>Science Advances</i>, vol. 9,
    no. 27, American Association for the Advancement of Science, 2023, p. eadg6983,
    doi:<a href="https://doi.org/10.1126/sciadv.adg6983">10.1126/sciadv.adg6983</a>.
  short: N. Ogura, Y. Sasagawa, T. Ito, T. Tameshige, S. Kawai, M. Sano, Y. Doll,
    A. Iwase, A. Kawamura, T. Suzuki, I. Nikaido, K. Sugimoto, M. Ikeuchi, Science
    Advances 9 (2023) eadg6983.
date_created: 2023-07-23T22:01:11Z
date_published: 2023-07-07T00:00:00Z
date_updated: 2023-12-13T11:59:29Z
day: '07'
ddc:
- '580'
doi: 10.1126/sciadv.adg6983
external_id:
  isi:
  - '001030983100012'
  pmid:
  - '37418524'
file:
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  creator: dernst
  date_created: 2023-08-01T06:40:35Z
  date_updated: 2023-08-01T06:40:35Z
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file_date_updated: 2023-08-01T06:40:35Z
has_accepted_license: '1'
intvolume: '         9'
isi: 1
issue: '27'
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
page: eadg6983
pmid: 1
publication: Science Advances
publication_identifier:
  eissn:
  - 2375-2548
publication_status: published
publisher: American Association for the Advancement of Science
quality_controlled: '1'
scopus_import: '1'
status: public
title: WUSCHEL-RELATED HOMEOBOX 13 suppresses de novo shoot regeneration via cell
  fate control of pluripotent callus
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 9
year: '2023'
...