@article{13259,
  abstract     = {Plants can regenerate their bodies via de novo establishment of shoot apical meristems (SAMs) from pluripotent callus. Only a small fraction of callus cells is eventually specified into SAMs but the molecular mechanisms underlying fate specification remain obscure. The expression of WUSCHEL (WUS) is an early hallmark of SAM fate acquisition. Here, we show that a WUS paralog, WUSCHEL-RELATED HOMEOBOX 13 (WOX13), negatively regulates SAM formation from callus in Arabidopsis thaliana. WOX13 promotes non-meristematic cell fate via transcriptional repression of WUS and other SAM regulators and activation of cell wall modifiers. Our Quartz-Seq2–based single cell transcriptome revealed that WOX13 plays key roles in determining cellular identity of callus cell population. We propose that reciprocal inhibition between WUS and WOX13 mediates critical cell fate determination in pluripotent cell population, which has a major impact on regeneration efficiency.},
  author       = {Ogura, Nao and Sasagawa, Yohei and Ito, Tasuku and Tameshige, Toshiaki and Kawai, Satomi and Sano, Masaki and Doll, Yuki and Iwase, Akira and Kawamura, Ayako and Suzuki, Takamasa and Nikaido, Itoshi and Sugimoto, Keiko and Ikeuchi, Momoko},
  issn         = {2375-2548},
  journal      = {Science Advances},
  number       = {27},
  pages        = {eadg6983},
  publisher    = {American Association for the Advancement of Science},
  title        = {{WUSCHEL-RELATED HOMEOBOX 13 suppresses de novo shoot regeneration via cell fate control of pluripotent callus}},
  doi          = {10.1126/sciadv.adg6983},
  volume       = {9},
  year         = {2023},
}