---
_id: '14786'
abstract:
- lang: eng
text: Acanthocephalans, intestinal parasites of vertebrates, are characterised by
orders of magnitude higher metal accumulation than free-living organisms, but
the mechanism of such effective metal accumulation is still unknown. The aim of
our study was to gain new insights into the high-resolution localization of elements
in the bodies of acanthocephalans, thus taking an initial step towards elucidating
metal uptake and accumulation in organisms under real environmental conditions.
For the first time, nanoscale secondary ion mass spectrometry (NanoSIMS) was used
for high-resolution mapping of 12 elements (C, Ca, Cu, Fe, N, Na, O, P, Pb, S,
Se, and Tl) in three selected body parts (trunk spines, inner part of the proboscis
receptacle and inner surface of the tegument) of Dentitruncus truttae, a parasite
of brown trout (Salmo trutta) from the Krka River in Croatia. In addition, the
same body parts were examined using transmission electron microscopy (TEM) and
correlated with NanoSIMS images. Metal concentrations determined using HR ICP-MS
confirmed higher accumulation in D. truttae than in the fish intestine. The chemical
composition of the acanthocephalan body showed the highest density of C, Ca, N,
Na, O, S, as important and constitutive elements in living cells in all studied
structures, while Fe was predominant among trace elements. In general, higher
element density was found in trunk spines and tegument, as body structures responsible
for substance absorption in parasites. The results obtained with NanoSIMS and
TEM-NanoSIMS correlative imaging represent pilot data for mapping of elements
at nanoscale resolution in the ultrastructure of various body parts of acanthocephalans
and generally provide a contribution for further application of this technique
in all parasite species.
acknowledgement: 'The authors thank the Czech Science Foundation (project No. 19-28399X)
and the Czech Academy of Sciences (RVO: 60077344) and are sincerely grateful to
the Bordeaux Imaging Centre (member of the France BioImaging national infrastructure,
ANR-10-INBS-04) for help with TEM and to members of the Laboratory of Biological
Effects of Metals and Laboratory of Aquaculture and Pathology of Aquatic Organisms
(Ruđer Bošković Institute, Croatia) for the assistance with fieldwork.'
article_number: '164010'
article_processing_charge: No
article_type: original
author:
- first_name: Vlatka
full_name: Filipović Marijić, Vlatka
last_name: Filipović Marijić
- first_name: Maria Angels
full_name: Subirana, Maria Angels
last_name: Subirana
- first_name: Dirk
full_name: Schaumlöffel, Dirk
last_name: Schaumlöffel
- first_name: Josip
full_name: Barišić, Josip
last_name: Barišić
- first_name: Etienne
full_name: Gontier, Etienne
last_name: Gontier
- first_name: Nesrete
full_name: Krasnici, Nesrete
id: cb5852d4-287f-11ed-baf0-bc1dd2d5c745
last_name: Krasnici
- first_name: Tatjana
full_name: Mijošek, Tatjana
last_name: Mijošek
- first_name: Jesús S.
full_name: Hernández-Orts, Jesús S.
last_name: Hernández-Orts
- first_name: Tomáš
full_name: Scholz, Tomáš
last_name: Scholz
- first_name: Marijana
full_name: Erk, Marijana
last_name: Erk
citation:
ama: Filipović Marijić V, Subirana MA, Schaumlöffel D, et al. First insight in element
localisation in different body parts of the acanthocephalan Dentitruncus truttae
using TEM and NanoSIMS. Science of The Total Environment. 2023;887. doi:10.1016/j.scitotenv.2023.164010
apa: Filipović Marijić, V., Subirana, M. A., Schaumlöffel, D., Barišić, J., Gontier,
E., Krasnici, N., … Erk, M. (2023). First insight in element localisation in different
body parts of the acanthocephalan Dentitruncus truttae using TEM and NanoSIMS.
Science of The Total Environment. Elsevier. https://doi.org/10.1016/j.scitotenv.2023.164010
chicago: Filipović Marijić, Vlatka, Maria Angels Subirana, Dirk Schaumlöffel, Josip
Barišić, Etienne Gontier, Nesrete Krasnici, Tatjana Mijošek, Jesús S. Hernández-Orts,
Tomáš Scholz, and Marijana Erk. “First Insight in Element Localisation in Different
Body Parts of the Acanthocephalan Dentitruncus Truttae Using TEM and NanoSIMS.”
Science of The Total Environment. Elsevier, 2023. https://doi.org/10.1016/j.scitotenv.2023.164010.
ieee: V. Filipović Marijić et al., “First insight in element localisation
in different body parts of the acanthocephalan Dentitruncus truttae using TEM
and NanoSIMS,” Science of The Total Environment, vol. 887. Elsevier, 2023.
ista: Filipović Marijić V, Subirana MA, Schaumlöffel D, Barišić J, Gontier E, Krasnici
N, Mijošek T, Hernández-Orts JS, Scholz T, Erk M. 2023. First insight in element
localisation in different body parts of the acanthocephalan Dentitruncus truttae
using TEM and NanoSIMS. Science of The Total Environment. 887, 164010.
mla: Filipović Marijić, Vlatka, et al. “First Insight in Element Localisation in
Different Body Parts of the Acanthocephalan Dentitruncus Truttae Using TEM and
NanoSIMS.” Science of The Total Environment, vol. 887, 164010, Elsevier,
2023, doi:10.1016/j.scitotenv.2023.164010.
short: V. Filipović Marijić, M.A. Subirana, D. Schaumlöffel, J. Barišić, E. Gontier,
N. Krasnici, T. Mijošek, J.S. Hernández-Orts, T. Scholz, M. Erk, Science of The
Total Environment 887 (2023).
date_created: 2024-01-10T10:43:08Z
date_published: 2023-08-20T00:00:00Z
date_updated: 2024-01-16T10:04:57Z
day: '20'
department:
- _id: LifeSc
doi: 10.1016/j.scitotenv.2023.164010
external_id:
isi:
- '001002645100001'
pmid:
- '37169189'
intvolume: ' 887'
isi: 1
keyword:
- Pollution
- Waste Management and Disposal
- Environmental Chemistry
- Environmental Engineering
language:
- iso: eng
month: '08'
oa_version: None
pmid: 1
publication: Science of The Total Environment
publication_identifier:
issn:
- 0048-9697
publication_status: published
publisher: Elsevier
quality_controlled: '1'
status: public
title: First insight in element localisation in different body parts of the acanthocephalan
Dentitruncus truttae using TEM and NanoSIMS
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 887
year: '2023'
...
---
_id: '12863'
abstract:
- lang: eng
text: In the present study, essential and nonessential metal content and biomarker
responses were investigated in the intestine of fish collected from the areas
polluted by mining. Our objective was to determine metal and biomarker levels
in tissue responsible for dietary intake, which is rarely studied in water pollution
research. The study was conducted in the Bregalnica River, reference location,
and in the Zletovska and Kriva Rivers (the Republic of North Macedonia), which
are directly influenced by the active mines Zletovo and Toranica, respectively.
Biological responses were analyzed in Vardar chub (Squalius vardarensis; Karaman,
1928), using for the first time intestinal cytosol as a potentially toxic cell
fraction, since metal sensitivity is mostly associated with cytosol. Cytosolic
metal levels were higher in fish under the influence of mining (Tl, Li, Cs, Mo,
Sr, Cd, Rb, and Cu in the Zletovska River and Cr, Pb, and Se in the Kriva River
compared to the Bregalnica River in both seasons). The same trend was evident
for total proteins, biomarkers of general stress, and metallothioneins, biomarkers
of metal exposure, indicating cellular disturbances in the intestine, the primary
site of dietary metal uptake. The association of cytosolic Cu and Cd at all locations
pointed to similar pathways and homeostasis of these metallothionein-binding metals.
Comparison with other indicator tissues showed that metal concentrations were
higher in the intestine of fish from mining-affected areas than in the liver and
gills. In general, these results indicated the importance of dietary metal pathways,
and cytosolic metal fraction in assessing pollution impacts in freshwater ecosystems.
acknowledgement: 'The authors are grateful to Dr. Nevenka Mikac for the opportunity
to perform metal measurements on HR ICP-MS. This research was funded by the Ministry
of Science, Education and Sport of the Republic of Croatia (projects No. 098–0982934-2721
and 098–1782739-2749). The sampling was carried out as a part of two Croatian-Macedonian
bilateral projects: “The assessment of availability and effects of metals on fish
in the rivers under the impact of mining activities” and “Bacterial and parasitical
communities of chub as indicators of the status of environment exposed to mining
activities.”'
article_processing_charge: No
article_type: original
author:
- first_name: Vlatka
full_name: Filipović Marijić, Vlatka
last_name: Filipović Marijić
- first_name: Nesrete
full_name: Krasnici, Nesrete
id: cb5852d4-287f-11ed-baf0-bc1dd2d5c745
last_name: Krasnici
- first_name: Damir
full_name: Valić, Damir
last_name: Valić
- first_name: Damir
full_name: Kapetanović, Damir
last_name: Kapetanović
- first_name: Irena
full_name: Vardić Smrzlić, Irena
last_name: Vardić Smrzlić
- first_name: Maja
full_name: Jordanova, Maja
last_name: Jordanova
- first_name: Katerina
full_name: Rebok, Katerina
last_name: Rebok
- first_name: Sheriban
full_name: Ramani, Sheriban
last_name: Ramani
- first_name: Vasil
full_name: Kostov, Vasil
last_name: Kostov
- first_name: Rodne
full_name: Nastova, Rodne
last_name: Nastova
- first_name: Zrinka
full_name: Dragun, Zrinka
last_name: Dragun
citation:
ama: Filipović Marijić V, Krasnici N, Valić D, et al. Pollution impact on metal
and biomarker responses in intestinal cytosol of freshwater fish. Environmental
Science and Pollution Research. 2023;30:63510-63521. doi:10.1007/s11356-023-26844-2
apa: Filipović Marijić, V., Krasnici, N., Valić, D., Kapetanović, D., Vardić Smrzlić,
I., Jordanova, M., … Dragun, Z. (2023). Pollution impact on metal and biomarker
responses in intestinal cytosol of freshwater fish. Environmental Science and
Pollution Research. Springer Nature. https://doi.org/10.1007/s11356-023-26844-2
chicago: Filipović Marijić, Vlatka, Nesrete Krasnici, Damir Valić, Damir Kapetanović,
Irena Vardić Smrzlić, Maja Jordanova, Katerina Rebok, et al. “Pollution Impact
on Metal and Biomarker Responses in Intestinal Cytosol of Freshwater Fish.” Environmental
Science and Pollution Research. Springer Nature, 2023. https://doi.org/10.1007/s11356-023-26844-2.
ieee: V. Filipović Marijić et al., “Pollution impact on metal and biomarker
responses in intestinal cytosol of freshwater fish,” Environmental Science
and Pollution Research, vol. 30. Springer Nature, pp. 63510–63521, 2023.
ista: Filipović Marijić V, Krasnici N, Valić D, Kapetanović D, Vardić Smrzlić I,
Jordanova M, Rebok K, Ramani S, Kostov V, Nastova R, Dragun Z. 2023. Pollution
impact on metal and biomarker responses in intestinal cytosol of freshwater fish.
Environmental Science and Pollution Research. 30, 63510–63521.
mla: Filipović Marijić, Vlatka, et al. “Pollution Impact on Metal and Biomarker
Responses in Intestinal Cytosol of Freshwater Fish.” Environmental Science
and Pollution Research, vol. 30, Springer Nature, 2023, pp. 63510–21, doi:10.1007/s11356-023-26844-2.
short: V. Filipović Marijić, N. Krasnici, D. Valić, D. Kapetanović, I. Vardić Smrzlić,
M. Jordanova, K. Rebok, S. Ramani, V. Kostov, R. Nastova, Z. Dragun, Environmental
Science and Pollution Research 30 (2023) 63510–63521.
date_created: 2023-04-23T22:01:03Z
date_published: 2023-05-01T00:00:00Z
date_updated: 2023-10-04T11:23:10Z
day: '01'
department:
- _id: LifeSc
doi: 10.1007/s11356-023-26844-2
external_id:
isi:
- '000970917900012'
pmid:
- '37055686'
intvolume: ' 30'
isi: 1
language:
- iso: eng
month: '05'
oa_version: None
page: 63510-63521
pmid: 1
publication: Environmental Science and Pollution Research
publication_identifier:
eissn:
- 1614-7499
issn:
- 0944-1344
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Pollution impact on metal and biomarker responses in intestinal cytosol of
freshwater fish
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 30
year: '2023'
...